RchiOBHm_Chr2g0118391

NDH shuttles electrons from NAD(P)H plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004593
Physical Location & Seq
Forward (+)
30710250 .. 30711401
1152 bp
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UTR
Exon/CDS
Intron
PRQ49121

Sequence Viewer

Length: 885 bp
ATGATCGATTCAACCTCTGATCAAAAAGATATACCTTGGTTATATTTCATCTCTTCAACAAGTTTAGTAATGAGCATAACGGCCCTATTATTCCGATGGAGAGAAGAAGCTACGATTAGCTTTTCGGGAAATTTTCAAACGAACAATTTCAACGAAATCTTTCAATTTCTTATTTTACTATGTTCAACTCTACGTATTCCTCTATCCGTAGAGTACATTGAATGTACAGAAATGGCTATTATAGAGTTTCTGTTATTCGTATTAACAGCTACTCTGGGAGGAATGTTTTTATGCAGTGCTAACGATTTAATAACTATCTTTGTAGCTCCAGAATGTTTCAGTTTATGCTCCTACCTATTATCTGGATATACCAAGAAAGATGTACGGTCTAATGAGGCTACTACGAAGTATTTACTCATGGGTGGGGCAAGCTCTTCTATTTTGGTTCATGGTTTCTCTTGGCTATATGGTTCATCTAGGGGGGAGATCGAGCTTCAAGAAATAGTGAATGGTCTTATCAATACACAAATGTATAACTCCCTAGGAATTTCAATTGCGCTTATATTCATCACTGTAGGAATTGGGTTCAAGCTTTCCCTAGCCCCTTCTCGTCAATGGACTCCTGACGTATATGAAGGAATGCTACATAGTTGGTTCTCATCCTTCAGAGACTACGAGTGTAATAGGAGCATCCGTCGACAAAAGGATCACCCTAAGATGATCATCTCATGGCTATTGAGAACGAATCAAATCAGATGGTTCTATTTCTCAATCTTTCTGACTTGCTCCTACGGAACCGAGGTCGAAAAGATTGAGAAAAATCAGTCATTCACAACCATTGATGAAGGATTCCTCGAAAATTTAAGGATTAGTAATCCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

294

Amino Acids

33.73

Weight (kDa)

5.21

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndh2_N PF19530 1 - 71 3e-32 NAD(P)H-quinone oxidoreductase subunit 2 N-terminal
Proton_antipo_M PF00361 100 - 213 1.6e-27 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 697
AclWI GGATC 1 cut(s) 714
AcsI RAATTY 3 cut(s) 130, 546, 859
AcuI CTGAAG 1 cut(s) 649
AfaI GTAC 3 cut(s) 215, 226, 384
AluBI AGCT 7 cut(s) 110, 120, 269, 326, 432, 493, 592
AluI AGCT 7 cut(s) 110, 120, 269, 326, 432, 493, 592
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 1 cut(s) 714
AoxI GGCC 1 cut(s) 81
ApoI RAATTY 3 cut(s) 130, 546, 859
Asp700I GAANNNNTTC 2 cut(s) 146, 159
AspA2I CCTAGG 1 cut(s) 541
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 701
AvrII CCTAGG 1 cut(s) 541
BarI GAAGNNNNNNTAC 2 cut(s) 627, 659
BccI CCATC 2 cut(s) 90, 750
BceAI ACGGC 1 cut(s) 96
BclI TGATCA 2 cut(s) 19, 720
BcoDI GTCTC 1 cut(s) 663
BfaI CTAG 3 cut(s) 477, 542, 599
BfmI CTRYAG 1 cut(s) 573
BlnI CCTAGG 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 796
BmsI GCATC 1 cut(s) 699
BpmI CTGGAG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 194
BsaBI GATNNNNATC 1 cut(s) 722
BsaJI CCNNGG 3 cut(s) 35, 541, 798
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bse8I GATNNNNATC 1 cut(s) 722
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 35, 541, 798
BseGI GGATG 2 cut(s) 659, 690
BseJI GATNNNNATC 1 cut(s) 722
BshFI GGCC 1 cut(s) 83
BshVI ATCGAT 1 cut(s) 6
BsmAI GTCTC 1 cut(s) 663
BsmI GAATGC 1 cut(s) 645
BsnI GGCC 1 cut(s) 83
Bsp1407I TGTACA 1 cut(s) 224
Bsp143I GATC 5 cut(s) 3, 19, 486, 706, 720
BspANI GGCC 1 cut(s) 83
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 1 cut(s) 796
BspPI GGATC 1 cut(s) 714
BspQI GCTCTTC 1 cut(s) 439
BsrGI TGTACA 1 cut(s) 224
BssECI CCNNGG 3 cut(s) 35, 541, 798
BssMI GATC 5 cut(s) 3, 19, 486, 706, 720
BssT1I CCWWGG 2 cut(s) 35, 541
Bst4CI ACNGT 2 cut(s) 387, 574
Bst6I CTCTTC 2 cut(s) 58, 439
BstAUI TGTACA 1 cut(s) 224
BstBAI YACGTR 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 1 cut(s) 714
BstF5I GGATG 2 cut(s) 659, 690
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 5 cut(s) 6, 22, 489, 709, 723
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 5 cut(s) 3, 19, 486, 706, 720
BstSFI CTRYAG 1 cut(s) 573
BstSNI TACGTA 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 1 cut(s) 83
BsuTUI ATCGAT 1 cut(s) 6
BtsCI GGATG 2 cut(s) 659, 690
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 301, 570
Cac8I GCNNGC 1 cut(s) 430
CfoI GCGC 1 cut(s) 559
Cfr13I GGNCC 1 cut(s) 82
ClaI ATCGAT 1 cut(s) 6
Csp6I GTAC 3 cut(s) 214, 225, 383
CviAII CATG 3 cut(s) 418, 449, 729
CviQI GTAC 3 cut(s) 214, 225, 383
DdeI CTNAG 1 cut(s) 714
DpnI GATC 5 cut(s) 5, 21, 488, 708, 722
DpnII GATC 5 cut(s) 3, 19, 486, 706, 720
Eam1104I CTCTTC 2 cut(s) 58, 439
EarI CTCTTC 2 cut(s) 58, 439
Eco105I TACGTA 1 cut(s) 194
Eco130I CCWWGG 2 cut(s) 35, 541
Eco57I CTGAAG 1 cut(s) 649
EcoT14I CCWWGG 2 cut(s) 35, 541
ErhI CCWWGG 2 cut(s) 35, 541
FaeI CATG 3 cut(s) 421, 452, 732
FatI CATG 3 cut(s) 417, 448, 728
FbaI TGATCA 2 cut(s) 19, 720
FblI GTMKAC 1 cut(s) 697
FokI GGATG 2 cut(s) 646, 677
FspBI CTAG 3 cut(s) 477, 542, 599
GlaI GCGC 1 cut(s) 558
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 1 cut(s) 83
HhaI GCGC 1 cut(s) 559
Hin1II CATG 3 cut(s) 421, 452, 732
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 1 cut(s) 698
HindII GTYRAC 1 cut(s) 698
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 4 cut(s) 8, 619, 745, 849
HphI GGTGA 1 cut(s) 701
Hpy166II GTNNAC 1 cut(s) 698
Hpy188I TCNGA 5 cut(s) 19, 95, 668, 755, 780
Hpy188III TCNNGA 5 cut(s) 126, 329, 363, 497, 623
Hpy8I GTNNAC 1 cut(s) 698
Hpy99I CGWCG 1 cut(s) 699
HpyAV CCTTC 4 cut(s) 615, 629, 673, 839
HpyCH4III ACNGT 2 cut(s) 387, 574
HpyCH4IV ACGT 2 cut(s) 193, 627
HpyCH4V TGCA 1 cut(s) 294
HpyF3I CTNAG 1 cut(s) 714
HpySE526I ACGT 2 cut(s) 193, 627
Hsp92II CATG 3 cut(s) 421, 452, 732
HspAI GCGC 1 cut(s) 557
Ksp22I TGATCA 2 cut(s) 19, 720
Kzo9I GATC 5 cut(s) 3, 19, 486, 706, 720
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 4 cut(s) 331, 353, 687, 791
LpnPI CCDG 4 cut(s) 260, 342, 348, 636
LweI GCATC 1 cut(s) 699
MaeI CTAG 3 cut(s) 477, 542, 599
MaeII ACGT 2 cut(s) 193, 627
MalI GATC 5 cut(s) 5, 21, 488, 708, 722
MboI GATC 5 cut(s) 3, 19, 486, 706, 720
MboII GAAGA 3 cut(s) 45, 116, 426
MfeI CAATTG 1 cut(s) 552
MluCI AATT 7 cut(s) 130, 145, 164, 546, 552, 579, 859
MlyI GAGTC 1 cut(s) 613
MnlI CCTC 6 cut(s) 25, 210, 272, 388, 793, 863
MroXI GAANNNNTTC 2 cut(s) 146, 159
MseI TTAA 3 cut(s) 263, 308, 863
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 645
NdeII GATC 5 cut(s) 3, 19, 486, 706, 720
NlaIII CATG 3 cut(s) 421, 452, 732
NlaIV GGNNCC 1 cut(s) 796
PciSI GCTCTTC 1 cut(s) 439
PctI GAATGC 1 cut(s) 645
PdmI GAANNNNTTC 2 cut(s) 146, 159
PfeI GAWTC 3 cut(s) 8, 745, 849
PleI GAGTC 1 cut(s) 613
PpsI GAGTC 1 cut(s) 613
Ppu21I YACGTR 1 cut(s) 194
PspN4I GGNNCC 1 cut(s) 796
PspPI GGNCC 1 cut(s) 82
RsaI GTAC 3 cut(s) 215, 226, 384
RsaNI GTAC 3 cut(s) 214, 225, 383
SalI GTCGAC 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 3 cut(s) 263, 308, 863
Sau3AI GATC 5 cut(s) 3, 19, 486, 706, 720
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 1 cut(s) 613
SfaNI GCATC 1 cut(s) 699
SfcI CTRYAG 1 cut(s) 573
SnaBI TACGTA 1 cut(s) 194
Sse9I AATT 7 cut(s) 130, 145, 164, 546, 552, 579, 859
SspMI CTAG 3 cut(s) 477, 542, 599
StyI CCWWGG 2 cut(s) 35, 541
TaaI ACNGT 2 cut(s) 387, 574
TaiI ACGT 2 cut(s) 196, 630
TaqI TCGA 5 cut(s) 6, 489, 697, 804, 855
TasI AATT 7 cut(s) 130, 145, 164, 546, 552, 579, 859
TatI WGTACW 2 cut(s) 213, 224
TfiI GAWTC 3 cut(s) 8, 745, 849
Tru1I TTAA 3 cut(s) 263, 308, 863
Tru9I TTAA 3 cut(s) 263, 308, 863
TscAI CASTG 2 cut(s) 301, 577
TspDTI ATGAA 6 cut(s) 37, 437, 462, 556, 648, 858
TspGWI ACGGA 3 cut(s) 196, 683, 807
TspRI CASTG 2 cut(s) 301, 577
XapI RAATTY 3 cut(s) 130, 546, 859
XmaJI CCTAGG 1 cut(s) 541
XmiI GTMKAC 1 cut(s) 697
XmnI GAANNNNTTC 2 cut(s) 146, 159
XspI CTAG 3 cut(s) 477, 542, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.