RchiOBHm_Chr1g0333111

Galactose oxidase, central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002311
Physical Location & Seq
Reverse (-)
25016469 .. 25022327
5859 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56195

Sequence Viewer

Length: 1155 bp
ATGCATTACTGGGTTCGAGCTTCTTCGTCTGATTTCGCCGGAACTCTTCCCCAACCTCGCAGTGGTCACACTTCCGTCAACATTGGAAAATCCAAAGTGATCGTGTTCGGAGGCCTTGTAGAGAAGAGGTTTCTCAACGATATCGTCGTCTATGACATTGATAATAAACTATGGTATAAGCCAGAGTGCACTGGCGGCACTGATGGACAAGTGGGTCCAAGTCCTCGGGCATTTCACGTTGCTGTTGTGATTGATTGTCATATGTTCATCTTTGGTGGGCGTTGCGGTGGCAAGAGGTTAGGTGACTTTTGGGTCCTAGATACTGATATATGGCAATGGTCAGAGTTGACAAGCTTTGGTGACTTACCCTCAGCACGAGACTTTGCTGCAGCTTCAGCTATTGGAAATCAGAAAATTATTATGTATGGTGGCTGGGATGGTAAAAAGTGGTTGTCAGATGTGTATGTCTTGGACACAATATCACTAGAGTGGATGGAACTGTCAGTTACTGGATCACTGCCACCACCTAGATGTGGCCACACAGCTACTATGGTTGAAAAACGGTTGCTTGTCTATGGCGGCAGAGGAGGTGGAGGTCCTATCATGGGTGATTTATGGGCTTTGAAGGGTGTAATCGCAGAAGAAAATGAAACACCTGGATGGACCCAACTGAAGCTTCCAGGTCAAGCTCCTTCTGCACGTTGTGGCCATACCATCACATCTGGAGGACACTATCTGTTGCTATTTGGAGGCCATGGAACTGGTGGCTGGTTGAGTCGTTATGACATCTATTACAATGACTGCATCATCTTAGACAGGGTTTCTGCACAGTGGAAGCGGTTACCTACTGGCAATGAACCCCCTCCTGCTCGAGCATACCATTCACTGACATGCATTGGCTCACGTCATCTGTTATTTGGTGGCTTTGATGGCAAATCCACATTTGGTGATCTATGGTGGTTGGTTCCTGAAGAGGACCCTATTGCAAACAGGCTTCGTGCAACTTCACCAACCAATATTACCGAAAATAAGGATGTCATGATGGAAAATAATAATGTCCAATCTGAACTCAAGGTATTAGTGCTACTGCCTCTAATTATGCCGATCATTCAATATGATTTTGCCAACGGCCATACTGCCTTACTTCTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4

Protein Analysis

384

Amino Acids

42.33

Weight (kDa)

6.26

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 10 - 194 7.1e-53 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 11 - 164 1.1e-30 FBX42, beta-propeller domain
Kelch_HCF PF13854 12 - 93 2.4e-11 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 72 - 137 2.3e-06 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 75 - 119 1.2e-09 Kelch motif
Kelch_HCF PF13854 90 - 145 1.9e-07 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 130 - 185 8.7e-06 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 137 - 209 1.3e-14 FBX42, beta-propeller domain
Kelch_HCF PF13854 137 - 194 9e-08 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 141 - 264 5.1e-11 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 217 - 323 7.4e-20 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 218 - 321 9.7e-16 FBX42, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 213, 311
AciI CCGC 4 cut(s) 195, 285, 579, 838
AclWI GGATC 1 cut(s) 520
AcoI YGGCCR 3 cut(s) 535, 706, 1129
AcuI CTGAAG 3 cut(s) 378, 692, 990
AdeI CACNNNGTG 1 cut(s) 704
AfiI CCNNNNNNNGG 3 cut(s) 62, 533, 605
AgsI TTSAA 3 cut(s) 557, 625, 1112
AjiI CACGTC 1 cut(s) 905
AjnI CCWGG 2 cut(s) 655, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AleI CACNNNNGTG 1 cut(s) 487
AluBI AGCT 7 cut(s) 20, 354, 392, 398, 545, 676, 689
AluI AGCT 7 cut(s) 20, 354, 392, 398, 545, 676, 689
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 1 cut(s) 372
Alw44I GTGCAC 1 cut(s) 187
AlwI GGATC 1 cut(s) 520
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 2 cut(s) 225, 870
AoxI GGCC 5 cut(s) 112, 535, 706, 751, 1129
ApaLI GTGCAC 1 cut(s) 187
ApeKI GCWGC 2 cut(s) 386, 389
AspS9I GGNCC 5 cut(s) 215, 313, 596, 663, 976
AsuHPI GGTGA 5 cut(s) 314, 371, 620, 959, 999
AvaI CYCGRG 2 cut(s) 225, 870
AvaII GGWCC 5 cut(s) 215, 313, 596, 663, 976
BaeGI GKGCMC 1 cut(s) 191
BalI TGGCCA 2 cut(s) 537, 708
BauI CACGAG 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 191
BbvCI CCTCAGC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 373, 401
BccI CCATC 7 cut(s) 197, 431, 487, 654, 722, 923, 1036
BceAI ACGGC 1 cut(s) 1144
BciT130I CCWGG 2 cut(s) 657, 681
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 3 cut(s) 317, 485, 528
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 4 cut(s) 196, 387, 390, 580
BlsI GCNGC 4 cut(s) 197, 388, 391, 581
Bme1390I CCNGG 2 cut(s) 657, 681
Bme18I GGWCC 5 cut(s) 215, 313, 596, 663, 976
BmeT110I CYCGRG 2 cut(s) 225, 870
BmgBI CACGTC 1 cut(s) 905
BmgT120I GGNCC 5 cut(s) 215, 313, 596, 663, 976
BmiI GGNNCC 5 cut(s) 216, 314, 665, 966, 978
BmrFI CCNGG 2 cut(s) 657, 681
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 1 cut(s) 813
BmuI ACTGGG 1 cut(s) 19
BpmI CTGGAG 1 cut(s) 744
Bpu10I CCTNAGC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 1055
BsaJI CCNNGG 2 cut(s) 224, 754
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 533, 605
Bse1I ACTGG 5 cut(s) 14, 196, 514, 766, 853
Bse3DI GCAATG 2 cut(s) 341, 859
BseBI CCWGG 2 cut(s) 657, 681
BseDI CCNNGG 2 cut(s) 224, 754
BseGI GGATG 4 cut(s) 442, 498, 665, 1039
BseLI CCNNNNNNNGG 3 cut(s) 62, 533, 605
BseMI GCAATG 2 cut(s) 341, 859
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 5 cut(s) 14, 196, 514, 766, 853
BseRI GAGGAG 1 cut(s) 600
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 2 cut(s) 373, 401
BseYI CCCAGC 1 cut(s) 432
BsgI GTGCAG 2 cut(s) 681, 810
BshFI GGCC 5 cut(s) 114, 537, 708, 753, 1131
BsiHKAI GWGCWC 1 cut(s) 191
BsiHKCI CYCGRG 2 cut(s) 225, 870
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 3 cut(s) 62, 533, 605
BsmAI GTCTC 1 cut(s) 372
BsnI GGCC 5 cut(s) 114, 537, 708, 753, 1131
BsoBI CYCGRG 2 cut(s) 225, 870
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 4 cut(s) 99, 512, 949, 1104
Bsp19I CCATGG 1 cut(s) 754
BspACI CCGC 4 cut(s) 195, 285, 579, 838
BspANI GGCC 5 cut(s) 114, 537, 708, 753, 1131
BspCNI CTCAG 1 cut(s) 383
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 5 cut(s) 216, 314, 665, 966, 978
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 1 cut(s) 520
BsrDI GCAATG 2 cut(s) 341, 859
BsrI ACTGG 5 cut(s) 14, 196, 514, 766, 853
BssECI CCNNGG 2 cut(s) 224, 754
BssMI GATC 4 cut(s) 99, 512, 949, 1104
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 754
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 2 cut(s) 657, 681
Bst4CI ACNGT 4 cut(s) 501, 564, 831, 1152
Bst6I CTCTTC 3 cut(s) 51, 119, 966
BstDEI CTNAG 2 cut(s) 370, 811
BstDSI CCRYGG 1 cut(s) 754
BstEII GGTNACC 1 cut(s) 840
BstF5I GGATG 4 cut(s) 442, 498, 665, 1039
BstKTI GATC 4 cut(s) 102, 515, 952, 1107
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 4 cut(s) 99, 512, 949, 1104
BstMWI GCNNNNNNNGC 4 cut(s) 195, 395, 695, 930
BstNI CCWGG 2 cut(s) 657, 681
BstNSI RCATGY 1 cut(s) 894
BstPI GGTNACC 1 cut(s) 840
BstSCI CCNGG 2 cut(s) 655, 679
BstSFI CTRYAG 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 373, 401
BstXI CCANNNNNNTGG 1 cut(s) 761
BsuRI GGCC 5 cut(s) 114, 537, 708, 753, 1131
BtgI CCRYGG 1 cut(s) 754
BtrI CACGTC 1 cut(s) 905
BtsCI GGATG 4 cut(s) 442, 498, 665, 1039
BtsI GCAGTG 2 cut(s) 67, 515
BtsIMutI CAGTG 6 cut(s) 67, 189, 198, 515, 836, 884
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 1038
Cfr13I GGNCC 5 cut(s) 215, 313, 596, 663, 976
CviAII CATG 4 cut(s) 604, 755, 891, 1039
DdeI CTNAG 2 cut(s) 370, 811
DpnI GATC 4 cut(s) 101, 514, 951, 1106
DpnII GATC 4 cut(s) 99, 512, 949, 1104
DraIII CACNNNGTG 1 cut(s) 704
DrdI GACNNNNNNGTC 2 cut(s) 213, 311
DseDI GACNNNNNNGTC 2 cut(s) 213, 311
EaeI YGGCCR 3 cut(s) 535, 706, 1129
Eam1104I CTCTTC 3 cut(s) 51, 119, 966
EarI CTCTTC 3 cut(s) 51, 119, 966
Eco130I CCWWGG 1 cut(s) 754
Eco147I AGGCCT 1 cut(s) 114
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 5 cut(s) 215, 313, 596, 663, 976
Eco57I CTGAAG 3 cut(s) 378, 692, 990
Eco88I CYCGRG 2 cut(s) 225, 870
Eco91I GGTNACC 1 cut(s) 840
EcoO109I RGGNCCY 3 cut(s) 313, 596, 976
EcoO65I GGTNACC 1 cut(s) 840
EcoRII CCWGG 2 cut(s) 655, 679
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 754
EcoT22I ATGCAT 2 cut(s) 6, 896
ErhI CCWWGG 1 cut(s) 754
FaeI CATG 4 cut(s) 607, 758, 894, 1042
FatI CATG 4 cut(s) 603, 754, 890, 1038
FauNDI CATATG 1 cut(s) 261
Fnu4HI GCNGC 4 cut(s) 196, 387, 390, 580
FokI GGATG 4 cut(s) 449, 505, 672, 1046
Fsp4HI GCNGC 4 cut(s) 196, 387, 390, 580
FspBI CTAG 3 cut(s) 317, 485, 528
GluI GCNGC 4 cut(s) 196, 387, 390, 580
GsaI CCCAGC 1 cut(s) 436
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 5 cut(s) 114, 537, 708, 753, 1131
HapII CCGG 1 cut(s) 39
Hin1II CATG 4 cut(s) 607, 758, 894, 1042
HincII GTYRAC 2 cut(s) 79, 348
HindII GTYRAC 2 cut(s) 79, 348
HindIII AAGCTT 2 cut(s) 352, 674
HinfI GANTC 1 cut(s) 775
HpaII CCGG 1 cut(s) 39
HphI GGTGA 5 cut(s) 314, 371, 620, 959, 999
Hpy166II GTNNAC 3 cut(s) 79, 189, 348
Hpy188I TCNGA 6 cut(s) 31, 110, 343, 411, 457, 1066
Hpy188III TCNNGA 3 cut(s) 723, 968, 1039
Hpy8I GTNNAC 3 cut(s) 79, 189, 348
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 2 cut(s) 619, 702
HpyCH4III ACNGT 4 cut(s) 501, 564, 831, 1152
HpyCH4IV ACGT 3 cut(s) 237, 700, 904
HpyCH4V TGCA 9 cut(s) 4, 189, 389, 698, 804, 827, 894, 986, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 195, 395, 695, 930
HpyF3I CTNAG 2 cut(s) 370, 811
HpySE526I ACGT 3 cut(s) 237, 700, 904
Hsp92II CATG 4 cut(s) 607, 758, 894, 1042
Kzo9I GATC 4 cut(s) 99, 512, 949, 1104
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 2 cut(s) 373, 401
LweI GCATC 1 cut(s) 813
MaeI CTAG 3 cut(s) 317, 485, 528
MaeII ACGT 3 cut(s) 237, 700, 904
MaeIII GTNAC 5 cut(s) 65, 302, 359, 505, 840
MalI GATC 4 cut(s) 101, 514, 951, 1106
MboI GATC 4 cut(s) 99, 512, 949, 1104
MboII GAAGA 5 cut(s) 15, 38, 136, 653, 983
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 2 cut(s) 537, 708
MluCI AATT 2 cut(s) 414, 1095
MluNI TGGCCA 2 cut(s) 537, 708
MlyI GAGTC 1 cut(s) 784
Mox20I TGGCCA 2 cut(s) 537, 708
Mph1103I ATGCAT 2 cut(s) 6, 896
MscI TGGCCA 2 cut(s) 537, 708
MslI CAYNNNNRTG 4 cut(s) 487, 529, 658, 889
Msp20I TGGCCA 2 cut(s) 537, 708
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 2 cut(s) 657, 681
MvaI CCWGG 2 cut(s) 657, 681
MwoI GCNNNNNNNGC 4 cut(s) 195, 395, 695, 930
NcoI CCATGG 1 cut(s) 754
NdeI CATATG 1 cut(s) 261
NdeII GATC 4 cut(s) 99, 512, 949, 1104
NlaIII CATG 4 cut(s) 607, 758, 894, 1042
NlaIV GGNNCC 5 cut(s) 216, 314, 665, 966, 978
NmuCI GTSAC 3 cut(s) 65, 302, 359
NsiI ATGCAT 2 cut(s) 6, 896
NspI RCATGY 1 cut(s) 894
OliI CACNNNNGTG 1 cut(s) 487
PaeR7I CTCGAG 1 cut(s) 870
PagI TCATGA 1 cut(s) 1038
PceI AGGCCT 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PkrI GCNGC 4 cut(s) 197, 388, 391, 581
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
PpuMI RGGWCCY 3 cut(s) 313, 596, 976
Psp5II RGGWCCY 3 cut(s) 313, 596, 976
Psp6I CCWGG 2 cut(s) 655, 679
PspEI GGTNACC 1 cut(s) 840
PspFI CCCAGC 1 cut(s) 432
PspGI CCWGG 2 cut(s) 655, 679
PspN4I GGNNCC 5 cut(s) 216, 314, 665, 966, 978
PspPI GGNCC 5 cut(s) 215, 313, 596, 663, 976
PspPPI RGGWCCY 3 cut(s) 313, 596, 976
PspXI VCTCGAGB 1 cut(s) 870
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 509
RseI CAYNNNNRTG 4 cut(s) 487, 529, 658, 889
SatI GCNGC 4 cut(s) 196, 387, 390, 580
Sau3AI GATC 4 cut(s) 99, 512, 949, 1104
Sau96I GGNCC 5 cut(s) 215, 313, 596, 663, 976
SchI GAGTC 1 cut(s) 784
ScrFI CCNGG 2 cut(s) 657, 681
SduI GDGCHC 1 cut(s) 191
SfaNI GCATC 1 cut(s) 813
SfcI CTRYAG 1 cut(s) 387
Sfr274I CTCGAG 1 cut(s) 870
SinI GGWCC 5 cut(s) 215, 313, 596, 663, 976
SlaI CTCGAG 1 cut(s) 870
SmiMI CAYNNNNRTG 4 cut(s) 487, 529, 658, 889
SmlI CTYRAG 2 cut(s) 870, 1070
SmoI CTYRAG 2 cut(s) 870, 1070
Sse9I AATT 2 cut(s) 414, 1095
SseBI AGGCCT 1 cut(s) 114
SsiI CCGC 4 cut(s) 195, 285, 579, 838
SspI AATATT 1 cut(s) 1018
SspMI CTAG 3 cut(s) 317, 485, 528
StuI AGGCCT 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 655, 679
StyI CCWWGG 1 cut(s) 754
TaaI ACNGT 4 cut(s) 501, 564, 831, 1152
TaiI ACGT 3 cut(s) 240, 703, 907
TaqI TCGA 2 cut(s) 16, 871
TasI AATT 2 cut(s) 414, 1095
TauI GCSGC 2 cut(s) 198, 582
TscAI CASTG 6 cut(s) 67, 196, 205, 522, 836, 891
TseFI GTSAC 3 cut(s) 65, 302, 359
TseI GCWGC 2 cut(s) 386, 389
Tsp45I GTSAC 3 cut(s) 65, 302, 359
TspDTI ATGAA 3 cut(s) 256, 663, 870
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 6 cut(s) 67, 196, 205, 522, 836, 891
VneI GTGCAC 1 cut(s) 187
VpaK11BI GGWCC 5 cut(s) 215, 313, 596, 663, 976
XceI RCATGY 1 cut(s) 894
XcmI CCANNNNNNNNNTGG 2 cut(s) 59, 761
XhoI CTCGAG 1 cut(s) 870
XspI CTAG 3 cut(s) 317, 485, 528
Zsp2I ATGCAT 2 cut(s) 6, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.