RchiOBHm_Chr1g0333981

Galactose oxidase, central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002311
Physical Location & Seq
Forward (+)
26100206 .. 26105537
5332 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56275

Sequence Viewer

Length: 1020 bp
ATGCATTACCGGGTTCGAGCTTCTTCGTCTGATTTCGCCGGAACTCTTCCCCAACCTCGCAGTGATCGTGTAGAGAAGAGGTTTCTCAACGATATCGTCGTCTATGACATTGATAATAAACTATGGTATAAGCCAGAGTGCACTGGCGGCACTGATGGACAAGTGGGTCCAAGTCCTCGGGCATTTCACGTTGCTGTTGTGATTGATTGTCATATGTTCATCTTTGGTGGGCGTTGCGGTGGCAAGAGGTTAGGTGACTTTTGGGTCCTAGATACTGATATATGGCAATGGTCAGAGTTGACAAGCTTTGGTGACTTACCCTCAGCACGCGACTTTGCTGCAGCTTCAGCTATTGGAAATCAGAAAATTATTATGTATGGTGGCTGGGATGGTAAAAAGTGGTTGTCAGATGTGTACGTCTTGGACACAATATCACTAGAGTGGATGGAACTGTCAGTTACTCGATCACTGCCACCACCTAGATGCGGCCACACAGCTACTATGGTTGAAAAACGGTTGCTTGTCTATGGCGGCACAGGAGGTGGAGGTCCTATCATGGGTGATTTATGGGCTTTGAAGGGTGTAATCGCAGAAGTTTCCCTTGGACTTTTCAAGATCAGTTTCGTTGAAGAAAATGAAACACCTGGATGGACCCAACTGAAGCTTCCAGGTCAAGCTCCTTCTGCACGTTGTGGCCATACCATCACATCTGGAGGACACTATCTGTTGCTATTTGGAGGCCATGGAACTGGTGGCTGGTTGAGTCGTTATGACATCTATTACAATGACTGCATCATCTTAGACAGGGTTTCTGCACAGTGGAAGCGGTTACCTACTGGCAATGAACCCCCTCCTGCTCGAGCATACCATTCACTGACATGCATTGGCTCACGTTATCTGGTATTTGGTGGCTTTGATGGCAAATCCACATTTGGTGATCTATGGTGGTTGGTTCCTGAAGAGGGACCCTATTGCAAACAGGCTTCGTGCAACTTCACCAACCAATATTACCGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4

Protein Analysis

339

Amino Acids

37.7

Weight (kDa)

7.06

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 18 - 148 3.2e-25 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 25 - 178 1.7e-42 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 27 - 120 3.2e-09 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 59 - 103 1e-09 Kelch motif
Kelch_HCF PF13854 80 - 129 2.2e-06 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 114 - 171 1.9e-06 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 115 - 193 8.9e-15 FBX42, beta-propeller domain
Kelch_HCF PF13854 121 - 178 1.1e-06 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 125 - 192 1.3e-07 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 213 - 319 7.7e-21 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 214 - 320 1.2e-16 FBX42, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 165, 263
AccII CGCG 1 cut(s) 330
AciI CCGC 5 cut(s) 147, 237, 486, 531, 826
AcoI YGGCCR 2 cut(s) 487, 694
AcuI CTGAAG 3 cut(s) 330, 680, 978
AdeI CACNNNGTG 1 cut(s) 692
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 3 cut(s) 485, 557, 962
AgsI TTSAA 4 cut(s) 509, 577, 613, 629
AjnI CCWGG 2 cut(s) 643, 667
AjuI GAANNNNNNNTTGG 4 cut(s) 585, 617, 648, 680
AleI CACNNNNGTG 1 cut(s) 439
AluBI AGCT 7 cut(s) 20, 306, 344, 350, 497, 664, 677
AluI AGCT 7 cut(s) 20, 306, 344, 350, 497, 664, 677
Alw21I GWGCWC 1 cut(s) 143
Alw44I GTGCAC 1 cut(s) 139
Ama87I CYCGRG 2 cut(s) 177, 858
AoxI GGCC 3 cut(s) 487, 694, 739
ApaLI GTGCAC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 338, 341
AspS9I GGNCC 5 cut(s) 167, 265, 548, 651, 965
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 5 cut(s) 266, 323, 572, 947, 988
AvaI CYCGRG 2 cut(s) 177, 858
AvaII GGWCC 5 cut(s) 167, 265, 548, 651, 965
BaeGI GKGCMC 1 cut(s) 143
BalI TGGCCA 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 143
BbvCI CCTCAGC 1 cut(s) 322
BbvI GCAGC 2 cut(s) 325, 353
BccI CCATC 6 cut(s) 149, 383, 439, 642, 710, 911
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BciT130I CCWGG 2 cut(s) 645, 669
BcnI CCSGG 1 cut(s) 11
BfaI CTAG 3 cut(s) 269, 437, 480
BfmI CTRYAG 1 cut(s) 339
BisI GCNGC 5 cut(s) 148, 339, 342, 487, 532
BlsI GCNGC 5 cut(s) 149, 340, 343, 488, 533
Bme1390I CCNGG 3 cut(s) 11, 645, 669
Bme18I GGWCC 5 cut(s) 167, 265, 548, 651, 965
BmeT110I CYCGRG 2 cut(s) 177, 858
BmgT120I GGNCC 5 cut(s) 167, 265, 548, 651, 965
BmiI GGNNCC 6 cut(s) 168, 266, 653, 954, 966, 967
BmrFI CCNGG 3 cut(s) 11, 645, 669
BmsI GCATC 2 cut(s) 473, 801
BpmI CTGGAG 1 cut(s) 732
Bpu10I CCTNAGC 1 cut(s) 322
BpuMI CCSGG 1 cut(s) 11
BsaJI CCNNGG 3 cut(s) 176, 601, 742
Bsc4I CCNNNNNNNGG 3 cut(s) 485, 557, 962
Bse1I ACTGG 3 cut(s) 148, 754, 841
Bse3DI GCAATG 2 cut(s) 293, 847
BseBI CCWGG 2 cut(s) 645, 669
BseDI CCNNGG 3 cut(s) 176, 601, 742
BseGI GGATG 3 cut(s) 394, 450, 653
BseLI CCNNNNNNNGG 3 cut(s) 485, 557, 962
BseMI GCAATG 2 cut(s) 293, 847
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 3 cut(s) 148, 754, 841
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 2 cut(s) 325, 353
BseYI CCCAGC 1 cut(s) 384
BsgI GTGCAG 2 cut(s) 669, 798
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 3 cut(s) 489, 696, 741
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 2 cut(s) 177, 858
BsiSI CCGG 2 cut(s) 10, 39
BslFI GGGAC 1 cut(s) 978
BslI CCNNNNNNNGG 3 cut(s) 485, 557, 962
BsmFI GGGAC 1 cut(s) 978
BsnI GGCC 3 cut(s) 489, 696, 741
BsoBI CYCGRG 2 cut(s) 177, 858
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 4 cut(s) 64, 464, 615, 937
Bsp19I CCATGG 1 cut(s) 742
BspACI CCGC 5 cut(s) 147, 237, 486, 531, 826
BspANI GGCC 3 cut(s) 489, 696, 741
BspCNI CTCAG 1 cut(s) 335
BspFNI CGCG 1 cut(s) 330
BspLI GGNNCC 6 cut(s) 168, 266, 653, 954, 966, 967
BspMAI CTGCAG 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 293, 847
BsrI ACTGG 3 cut(s) 148, 754, 841
BssECI CCNNGG 3 cut(s) 176, 601, 742
BssMI GATC 4 cut(s) 64, 464, 615, 937
BssT1I CCWWGG 2 cut(s) 601, 742
Bst2UI CCWGG 2 cut(s) 645, 669
Bst4CI ACNGT 3 cut(s) 453, 516, 819
Bst6I CTCTTC 3 cut(s) 51, 71, 954
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 2 cut(s) 322, 799
BstDSI CCRYGG 1 cut(s) 742
BstEII GGTNACC 1 cut(s) 828
BstF5I GGATG 3 cut(s) 394, 450, 653
BstFNI CGCG 1 cut(s) 330
BstKTI GATC 4 cut(s) 67, 467, 618, 940
BstMBI GATC 4 cut(s) 64, 464, 615, 937
BstMWI GCNNNNNNNGC 4 cut(s) 147, 347, 683, 918
BstNI CCWGG 2 cut(s) 645, 669
BstNSI RCATGY 1 cut(s) 882
BstPI GGTNACC 1 cut(s) 828
BstSCI CCNGG 3 cut(s) 9, 643, 667
BstSFI CTRYAG 1 cut(s) 339
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 325, 353
BstXI CCANNNNNNTGG 1 cut(s) 749
BsuRI GGCC 3 cut(s) 489, 696, 741
BtgI CCRYGG 1 cut(s) 742
BtsCI GGATG 3 cut(s) 394, 450, 653
BtsI GCAGTG 2 cut(s) 67, 467
BtsIMutI CAGTG 6 cut(s) 67, 141, 150, 467, 824, 872
Cac8I GCNNGC 1 cut(s) 328
Cfr13I GGNCC 5 cut(s) 167, 265, 548, 651, 965
Csp6I GTAC 1 cut(s) 415
CviAII CATG 3 cut(s) 556, 743, 879
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 2 cut(s) 322, 799
DpnI GATC 4 cut(s) 66, 466, 617, 939
DpnII GATC 4 cut(s) 64, 464, 615, 937
DraIII CACNNNGTG 1 cut(s) 692
DrdI GACNNNNNNGTC 2 cut(s) 165, 263
DseDI GACNNNNNNGTC 2 cut(s) 165, 263
EaeI YGGCCR 2 cut(s) 487, 694
Eam1104I CTCTTC 3 cut(s) 51, 71, 954
EarI CTCTTC 3 cut(s) 51, 71, 954
Eco130I CCWWGG 2 cut(s) 601, 742
Eco32I GATATC 1 cut(s) 94
Eco47I GGWCC 5 cut(s) 167, 265, 548, 651, 965
Eco57I CTGAAG 3 cut(s) 330, 680, 978
Eco88I CYCGRG 2 cut(s) 177, 858
Eco91I GGTNACC 1 cut(s) 828
EcoO109I RGGNCCY 3 cut(s) 265, 548, 965
EcoO65I GGTNACC 1 cut(s) 828
EcoRII CCWGG 2 cut(s) 643, 667
EcoRV GATATC 1 cut(s) 94
EcoT14I CCWWGG 2 cut(s) 601, 742
EcoT22I ATGCAT 2 cut(s) 6, 884
ErhI CCWWGG 2 cut(s) 601, 742
FaeI CATG 3 cut(s) 559, 746, 882
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 1 cut(s) 978
FatI CATG 3 cut(s) 555, 742, 878
FauNDI CATATG 1 cut(s) 213
Fnu4HI GCNGC 5 cut(s) 148, 339, 342, 487, 532
FokI GGATG 3 cut(s) 401, 457, 660
Fsp4HI GCNGC 5 cut(s) 148, 339, 342, 487, 532
FspBI CTAG 3 cut(s) 269, 437, 480
GluI GCNGC 5 cut(s) 148, 339, 342, 487, 532
GsaI CCCAGC 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 3 cut(s) 489, 696, 741
HapII CCGG 2 cut(s) 10, 39
Hin1II CATG 3 cut(s) 559, 746, 882
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 2 cut(s) 304, 662
HinfI GANTC 1 cut(s) 763
HpaII CCGG 2 cut(s) 10, 39
HphI GGTGA 5 cut(s) 266, 323, 572, 947, 988
Hpy166II GTNNAC 3 cut(s) 141, 300, 415
Hpy188I TCNGA 4 cut(s) 31, 295, 363, 409
Hpy188III TCNNGA 3 cut(s) 613, 711, 956
Hpy8I GTNNAC 3 cut(s) 141, 300, 415
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 571, 690
HpyCH4III ACNGT 3 cut(s) 453, 516, 819
HpyCH4IV ACGT 4 cut(s) 189, 417, 688, 892
HpyCH4V TGCA 9 cut(s) 4, 141, 341, 686, 792, 815, 882, 975, 990
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 347, 683, 918
HpyF3I CTNAG 2 cut(s) 322, 799
HpySE526I ACGT 4 cut(s) 189, 417, 688, 892
Hsp92II CATG 3 cut(s) 559, 746, 882
KflI GGGWCCC 1 cut(s) 965
Kzo9I GATC 4 cut(s) 64, 464, 615, 937
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 2 cut(s) 325, 353
LweI GCATC 2 cut(s) 473, 801
MaeI CTAG 3 cut(s) 269, 437, 480
MaeII ACGT 4 cut(s) 189, 417, 688, 892
MaeIII GTNAC 4 cut(s) 254, 311, 457, 828
MalI GATC 4 cut(s) 66, 466, 617, 939
MboI GATC 4 cut(s) 64, 464, 615, 937
MboII GAAGA 5 cut(s) 15, 38, 88, 641, 971
MhlI GDGCHC 1 cut(s) 143
MlsI TGGCCA 1 cut(s) 696
MluCI AATT 1 cut(s) 366
MluNI TGGCCA 1 cut(s) 696
MlyI GAGTC 1 cut(s) 772
Mox20I TGGCCA 1 cut(s) 696
Mph1103I ATGCAT 2 cut(s) 6, 884
MscI TGGCCA 1 cut(s) 696
MslI CAYNNNNRTG 4 cut(s) 439, 481, 646, 877
Msp20I TGGCCA 1 cut(s) 696
MspI CCGG 2 cut(s) 10, 39
MspR9I CCNGG 3 cut(s) 11, 645, 669
MvaI CCWGG 2 cut(s) 645, 669
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 4 cut(s) 147, 347, 683, 918
NciI CCSGG 1 cut(s) 11
NcoI CCATGG 1 cut(s) 742
NdeI CATATG 1 cut(s) 213
NdeII GATC 4 cut(s) 64, 464, 615, 937
NlaIII CATG 3 cut(s) 559, 746, 882
NlaIV GGNNCC 6 cut(s) 168, 266, 653, 954, 966, 967
NmuCI GTSAC 2 cut(s) 254, 311
NsiI ATGCAT 2 cut(s) 6, 884
NspI RCATGY 1 cut(s) 882
OliI CACNNNNGTG 1 cut(s) 439
PaeR7I CTCGAG 1 cut(s) 858
PcsI WCGNNNNNNNCGW 2 cut(s) 64, 96
PkrI GCNGC 5 cut(s) 149, 340, 343, 488, 533
PleI GAGTC 1 cut(s) 771
PpsI GAGTC 1 cut(s) 771
PpuMI RGGWCCY 3 cut(s) 265, 548, 965
Psp5II RGGWCCY 3 cut(s) 265, 548, 965
Psp6I CCWGG 2 cut(s) 643, 667
PspEI GGTNACC 1 cut(s) 828
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 2 cut(s) 643, 667
PspN4I GGNNCC 6 cut(s) 168, 266, 653, 954, 966, 967
PspPI GGNCC 5 cut(s) 167, 265, 548, 651, 965
PspPPI RGGWCCY 3 cut(s) 265, 548, 965
PspXI VCTCGAGB 1 cut(s) 858
PstI CTGCAG 1 cut(s) 343
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 4 cut(s) 439, 481, 646, 877
SatI GCNGC 5 cut(s) 148, 339, 342, 487, 532
Sau3AI GATC 4 cut(s) 64, 464, 615, 937
Sau96I GGNCC 5 cut(s) 167, 265, 548, 651, 965
SchI GAGTC 1 cut(s) 772
ScrFI CCNGG 3 cut(s) 11, 645, 669
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 2 cut(s) 473, 801
SfcI CTRYAG 1 cut(s) 339
Sfr274I CTCGAG 1 cut(s) 858
SinI GGWCC 5 cut(s) 167, 265, 548, 651, 965
SlaI CTCGAG 1 cut(s) 858
SmiMI CAYNNNNRTG 4 cut(s) 439, 481, 646, 877
SmlI CTYRAG 1 cut(s) 858
SmoI CTYRAG 1 cut(s) 858
Sse9I AATT 1 cut(s) 366
SsiI CCGC 5 cut(s) 147, 237, 486, 531, 826
SspI AATATT 1 cut(s) 1007
SspMI CTAG 3 cut(s) 269, 437, 480
StyD4I CCNGG 3 cut(s) 9, 643, 667
StyI CCWWGG 2 cut(s) 601, 742
TaaI ACNGT 3 cut(s) 453, 516, 819
TaiI ACGT 4 cut(s) 192, 420, 691, 895
TaqI TCGA 3 cut(s) 16, 463, 859
TasI AATT 1 cut(s) 366
TauI GCSGC 3 cut(s) 150, 489, 534
TscAI CASTG 6 cut(s) 67, 148, 157, 474, 824, 879
TseFI GTSAC 2 cut(s) 254, 311
TseI GCWGC 2 cut(s) 338, 341
Tsp45I GTSAC 2 cut(s) 254, 311
TspDTI ATGAA 3 cut(s) 208, 651, 858
TspRI CASTG 6 cut(s) 67, 148, 157, 474, 824, 879
VneI GTGCAC 1 cut(s) 139
VpaK11BI GGWCC 5 cut(s) 167, 265, 548, 651, 965
XceI RCATGY 1 cut(s) 882
XcmI CCANNNNNNNNNTGG 1 cut(s) 749
XhoI CTCGAG 1 cut(s) 858
XspI CTAG 3 cut(s) 269, 437, 480
Zsp2I ATGCAT 2 cut(s) 6, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.