Rw1G010260

Galactose oxidase, central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002311
Physical Location & Seq
Forward (+)
22968495 .. 22975875
7381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010260.1

Sequence Viewer

Length: 1362 bp
ATGCATTACTGGGTTCGAGCTTGTTCGTCTGATTTCGCCGGAACTCTTCCCCAACCTCGCAGTGGTCACACTTCCGTCAACATTGGAAAATCCAAAGTGATCGTGTTCGGAGGCCTTGTAGAGAAGAGGTTTCTCAACGATATCGTCGTCTATGACATTGATAATAAACTATGGTATAAGCCAGAGTGCACTGGCGGCACTGATGGACAAGTGGGTCCAAGTCCTCGGGCATTTCACGTTGCTGTTGTGATTGATTGTCATATGTTCATCTTTGGTGGGCGTTGCGGTGGCAAGAGGTTAGGTGACTTTTGGGTCCTAGATACTGATATATGGCAATGGTCAGAGTTGACAAGCTTTGGTGACTTACCCTCAGCACGCGACTTTGCTGCAGCTTCAGCTATTGGAAATCAGAAAATTATTATGTATGGTGGCTGGGATGGTAAAAAGTGGTTGTCAGATGTGTACGTCTTGGACACAATATCACTAGAGTGGATGGAACTGTCAGTTACTGGATCACTGCCACCACCTAGATGTGGCCACACAGCTACTATGGTTGAAAAACGGTTGCTTGTCTATGGCGGCAGAGGAGGTGGAGGTCCTATCATGGGTGATTTATGGGCTTTGAAGGGTGTAATCGCAGAAGAAAATGAAACACCTGGATGGACCCAACTGAAGCTTCCAGGTCAAGCTCCTTCTGCACGTTGTGGCCATACCATCACATCTGGAGGACACTATCTGTTGCTATTTGGAGGCCATGGAACTGGTGGCTGGTTGAGTCGTTATGACATCTATTACAATGACTGCATCATCTTAGACAGGGTTTCTGCACAGTGGAAGCGGTTACCTACTGGCAATGAACCCCCTCCTGCTCGAGCATACCATTCACTGACATGCATTGGCTCACGTTATCTGTTATTTGGTGGCTTTGATGGCAAATCCACATTTGGTGATCTATGGTGGTTGGTTCCTGAAGAGGACCCTATTGCAAACAGGCTTCGTGCAACTTCACCAACCAATATTACCGAAAATAAGGATGTCATGATGGAAAATAATAATGTCCAATCTGAACTCAAGGAAAGCGAAACAGAAGAATCTGCTGTCTCAGAATTGCGAAAAAGATTAGGAATATCAGTTTCTCTCCCTAGTAATGGGGTTCCTGTTGTGGATGAGTTAGAAGACAGAGAACTTATTGAACTAGCTTCAAGTTTGGTTGGAGAAAGAGATGCTACTCGTGAACATATTTCACATATTCAGGCACTTCGTGACCACTGGAGGAATTGTACTGCAAGGTTCATACCACTTAAAGAGCTTGGGCCTTTGCTTCGGGACTACCAACGCCAAATTACTCGTCGTCACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4

Protein Analysis

453

Amino Acids

50.42

Weight (kDa)

6.13

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 11 - 194 1.5e-52 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 12 - 164 1.6e-30 FBX42, beta-propeller domain
Kelch_HCF PF13854 12 - 93 3.4e-11 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 72 - 137 3.1e-06 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 75 - 119 1.5e-09 Kelch motif
Kelch_HCF PF13854 96 - 145 3.7e-06 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 131 - 209 2.8e-15 FBX42, beta-propeller domain
Kelch_HCF PF13854 136 - 194 8.8e-08 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 141 - 264 6.9e-11 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 204 - 322 2.2e-16 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 217 - 323 3.7e-20 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 213, 311
AccII CGCG 1 cut(s) 378
AciI CCGC 4 cut(s) 195, 285, 579, 838
AclWI GGATC 1 cut(s) 520
AcoI YGGCCR 2 cut(s) 535, 706
AcuI CTGAAG 3 cut(s) 378, 692, 990
AdeI CACNNNGTG 2 cut(s) 704, 1262
AfaI GTAC 2 cut(s) 464, 1282
AfiI CCNNNNNNNGG 4 cut(s) 62, 533, 605, 1148
AgsI TTSAA 4 cut(s) 557, 625, 1193, 1203
AjnI CCWGG 2 cut(s) 655, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AleI CACNNNNGTG 1 cut(s) 487
AluBI AGCT 9 cut(s) 20, 354, 392, 398, 545, 676, 689, 1199, 1309
AluI AGCT 9 cut(s) 20, 354, 392, 398, 545, 676, 689, 1199, 1309
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 1 cut(s) 1105
Alw44I GTGCAC 1 cut(s) 187
AlwI GGATC 1 cut(s) 520
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 2 cut(s) 225, 870
AoxI GGCC 5 cut(s) 112, 535, 706, 751, 1313
ApaLI GTGCAC 1 cut(s) 187
ApeKI GCWGC 2 cut(s) 386, 389
ArsI GACNNNNNNTTYG 1 cut(s) 1333
AspS9I GGNCC 6 cut(s) 215, 313, 596, 663, 976, 1313
AsuHPI GGTGA 6 cut(s) 314, 371, 620, 959, 999, 1346
AvaI CYCGRG 2 cut(s) 225, 870
AvaII GGWCC 5 cut(s) 215, 313, 596, 663, 976
BaeGI GKGCMC 1 cut(s) 191
BalI TGGCCA 2 cut(s) 537, 708
BauI CACGAG 1 cut(s) 1230
BbsI GAAGAC 1 cut(s) 1182
Bbv12I GWGCWC 1 cut(s) 191
BbvCI CCTCAGC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 373, 401
BccI CCATC 7 cut(s) 197, 431, 487, 654, 722, 923, 1036
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 2 cut(s) 657, 681
BcoDI GTCTC 1 cut(s) 1105
BfaI CTAG 5 cut(s) 317, 485, 528, 1143, 1196
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 4 cut(s) 196, 387, 390, 580
BlsI GCNGC 4 cut(s) 197, 388, 391, 581
Bme1390I CCNGG 2 cut(s) 657, 681
Bme18I GGWCC 5 cut(s) 215, 313, 596, 663, 976
BmeT110I CYCGRG 2 cut(s) 225, 870
BmgT120I GGNCC 6 cut(s) 215, 313, 596, 663, 976, 1313
BmiI GGNNCC 6 cut(s) 216, 314, 665, 966, 978, 1155
BmrFI CCNGG 2 cut(s) 657, 681
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 2 cut(s) 813, 1213
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 1182
BpmI CTGGAG 2 cut(s) 744, 1291
Bpu10I CCTNAGC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 1055
BsaJI CCNNGG 2 cut(s) 224, 754
BsaXI ACNNNNNCTCC 4 cut(s) 102, 132, 1264, 1294
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 533, 605, 1148
Bse1I ACTGG 6 cut(s) 14, 196, 514, 766, 853, 1274
Bse3DI GCAATG 2 cut(s) 341, 859
BseBI CCWGG 2 cut(s) 657, 681
BseDI CCNNGG 2 cut(s) 224, 754
BseGI GGATG 5 cut(s) 442, 498, 665, 1039, 1171
BseLI CCNNNNNNNGG 4 cut(s) 62, 533, 605, 1148
BseMI GCAATG 2 cut(s) 341, 859
BseMII CTCAG 2 cut(s) 384, 1116
BseNI ACTGG 6 cut(s) 14, 196, 514, 766, 853, 1274
BseRI GAGGAG 1 cut(s) 600
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 2 cut(s) 373, 401
BseYI CCCAGC 1 cut(s) 432
BsgI GTGCAG 2 cut(s) 681, 810
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 5 cut(s) 114, 537, 708, 753, 1315
BsiHKAI GWGCWC 1 cut(s) 191
BsiHKCI CYCGRG 2 cut(s) 225, 870
BsiSI CCGG 1 cut(s) 39
BslFI GGGAC 1 cut(s) 1340
BslI CCNNNNNNNGG 4 cut(s) 62, 533, 605, 1148
BsmAI GTCTC 1 cut(s) 1105
BsmFI GGGAC 1 cut(s) 1340
BsnI GGCC 5 cut(s) 114, 537, 708, 753, 1315
BsoBI CYCGRG 2 cut(s) 225, 870
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 3 cut(s) 99, 512, 949
Bsp19I CCATGG 1 cut(s) 754
BspACI CCGC 4 cut(s) 195, 285, 579, 838
BspANI GGCC 5 cut(s) 114, 537, 708, 753, 1315
BspCNI CTCAG 2 cut(s) 383, 1115
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 6 cut(s) 216, 314, 665, 966, 978, 1155
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 1 cut(s) 520
BsrDI GCAATG 2 cut(s) 341, 859
BsrI ACTGG 6 cut(s) 14, 196, 514, 766, 853, 1274
BssECI CCNNGG 2 cut(s) 224, 754
BssMI GATC 3 cut(s) 99, 512, 949
BssSI CACGAG 1 cut(s) 1230
BssT1I CCWWGG 1 cut(s) 754
Bst2BI CACGAG 1 cut(s) 1230
Bst2UI CCWGG 2 cut(s) 657, 681
Bst4CI ACNGT 3 cut(s) 501, 564, 831
Bst6I CTCTTC 3 cut(s) 51, 119, 966
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 3 cut(s) 370, 811, 1102
BstDSI CCRYGG 1 cut(s) 754
BstEII GGTNACC 1 cut(s) 840
BstF5I GGATG 5 cut(s) 442, 498, 665, 1039, 1171
BstFNI CGCG 1 cut(s) 378
BstKTI GATC 3 cut(s) 102, 515, 952
BstMAI GTCTC 1 cut(s) 1105
BstMBI GATC 3 cut(s) 99, 512, 949
BstMWI GCNNNNNNNGC 4 cut(s) 195, 395, 695, 930
BstNI CCWGG 2 cut(s) 657, 681
BstNSI RCATGY 1 cut(s) 894
BstPI GGTNACC 1 cut(s) 840
BstSCI CCNGG 2 cut(s) 655, 679
BstSFI CTRYAG 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 191
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 2 cut(s) 373, 401
BstV2I GAAGAC 1 cut(s) 1182
BstXI CCANNNNNNTGG 1 cut(s) 761
BsuRI GGCC 5 cut(s) 114, 537, 708, 753, 1315
BtgI CCRYGG 1 cut(s) 754
BtsCI GGATG 5 cut(s) 442, 498, 665, 1039, 1171
BtsI GCAGTG 2 cut(s) 67, 515
BtsIMutI CAGTG 7 cut(s) 67, 189, 198, 515, 836, 884, 1267
Cac8I GCNNGC 1 cut(s) 376
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 1038
Cfr13I GGNCC 6 cut(s) 215, 313, 596, 663, 976, 1313
Csp6I GTAC 2 cut(s) 463, 1281
CviAII CATG 4 cut(s) 604, 755, 891, 1039
CviQI GTAC 2 cut(s) 463, 1281
DdeI CTNAG 3 cut(s) 370, 811, 1102
DpnI GATC 3 cut(s) 101, 514, 951
DpnII GATC 3 cut(s) 99, 512, 949
DraIII CACNNNGTG 2 cut(s) 704, 1262
DrdI GACNNNNNNGTC 2 cut(s) 213, 311
DseDI GACNNNNNNGTC 2 cut(s) 213, 311
EaeI YGGCCR 2 cut(s) 535, 706
Eam1104I CTCTTC 3 cut(s) 51, 119, 966
EarI CTCTTC 3 cut(s) 51, 119, 966
Eco130I CCWWGG 1 cut(s) 754
Eco147I AGGCCT 1 cut(s) 114
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 5 cut(s) 215, 313, 596, 663, 976
Eco57I CTGAAG 3 cut(s) 378, 692, 990
Eco88I CYCGRG 2 cut(s) 225, 870
Eco91I GGTNACC 1 cut(s) 840
EcoO109I RGGNCCY 3 cut(s) 313, 596, 976
EcoO65I GGTNACC 1 cut(s) 840
EcoRII CCWGG 2 cut(s) 655, 679
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 754
EcoT22I ATGCAT 2 cut(s) 6, 896
ErhI CCWWGG 1 cut(s) 754
FaeI CATG 4 cut(s) 607, 758, 894, 1042
FaqI GGGAC 1 cut(s) 1340
FatI CATG 4 cut(s) 603, 754, 890, 1038
FauNDI CATATG 1 cut(s) 261
Fnu4HI GCNGC 4 cut(s) 196, 387, 390, 580
FokI GGATG 5 cut(s) 449, 505, 672, 1046, 1178
Fsp4HI GCNGC 4 cut(s) 196, 387, 390, 580
FspBI CTAG 5 cut(s) 317, 485, 528, 1143, 1196
GluI GCNGC 4 cut(s) 196, 387, 390, 580
GsaI CCCAGC 1 cut(s) 436
GsuI CTGGAG 2 cut(s) 744, 1291
HaeIII GGCC 5 cut(s) 114, 537, 708, 753, 1315
HapII CCGG 1 cut(s) 39
Hin1II CATG 4 cut(s) 607, 758, 894, 1042
HincII GTYRAC 2 cut(s) 79, 348
HindII GTYRAC 2 cut(s) 79, 348
HindIII AAGCTT 2 cut(s) 352, 674
HinfI GANTC 2 cut(s) 775, 1091
HpaII CCGG 1 cut(s) 39
HphI GGTGA 6 cut(s) 314, 371, 620, 959, 999, 1346
Hpy166II GTNNAC 5 cut(s) 79, 189, 348, 463, 1235
Hpy188I TCNGA 7 cut(s) 31, 110, 343, 411, 457, 1066, 1105
Hpy188III TCNNGA 6 cut(s) 723, 968, 1039, 1232, 1262, 1325
Hpy8I GTNNAC 5 cut(s) 79, 189, 348, 463, 1235
Hpy99I CGWCG 2 cut(s) 149, 1353
HpyAV CCTTC 2 cut(s) 619, 702
HpyCH4III ACNGT 3 cut(s) 501, 564, 831
HpyCH4IV ACGT 4 cut(s) 237, 465, 700, 904
HpyF10VI GCNNNNNNNGC 4 cut(s) 195, 395, 695, 930
HpyF3I CTNAG 3 cut(s) 370, 811, 1102
HpySE526I ACGT 4 cut(s) 237, 465, 700, 904
Hsp92II CATG 4 cut(s) 607, 758, 894, 1042
Kzo9I GATC 3 cut(s) 99, 512, 949
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 2 cut(s) 373, 401
LweI GCATC 2 cut(s) 813, 1213
MaeI CTAG 5 cut(s) 317, 485, 528, 1143, 1196
MaeII ACGT 4 cut(s) 237, 465, 700, 904
MaeIII GTNAC 7 cut(s) 65, 302, 359, 505, 840, 1262, 1352
MalI GATC 3 cut(s) 101, 514, 951
MboI GATC 3 cut(s) 99, 512, 949
MboII GAAGA 6 cut(s) 38, 136, 653, 983, 1100, 1187
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 2 cut(s) 537, 708
MluCI AATT 4 cut(s) 414, 1106, 1276, 1341
MluNI TGGCCA 2 cut(s) 537, 708
MlyI GAGTC 1 cut(s) 784
MmeI TCCRAC 1 cut(s) 1192
Mox20I TGGCCA 2 cut(s) 537, 708
Mph1103I ATGCAT 2 cut(s) 6, 896
MscI TGGCCA 2 cut(s) 537, 708
MseI TTAA 1 cut(s) 1302
MslI CAYNNNNRTG 4 cut(s) 487, 529, 658, 889
Msp20I TGGCCA 2 cut(s) 537, 708
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 2 cut(s) 657, 681
MvaI CCWGG 2 cut(s) 657, 681
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 4 cut(s) 195, 395, 695, 930
NcoI CCATGG 1 cut(s) 754
NdeI CATATG 1 cut(s) 261
NdeII GATC 3 cut(s) 99, 512, 949
NlaIII CATG 4 cut(s) 607, 758, 894, 1042
NlaIV GGNNCC 6 cut(s) 216, 314, 665, 966, 978, 1155
NmuCI GTSAC 5 cut(s) 65, 302, 359, 1262, 1352
NsiI ATGCAT 2 cut(s) 6, 896
NspI RCATGY 1 cut(s) 894
OliI CACNNNNGTG 1 cut(s) 487
PaeR7I CTCGAG 1 cut(s) 870
PagI TCATGA 1 cut(s) 1038
PceI AGGCCT 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PfeI GAWTC 1 cut(s) 1091
PkrI GCNGC 4 cut(s) 197, 388, 391, 581
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
PpuMI RGGWCCY 3 cut(s) 313, 596, 976
Psp5II RGGWCCY 3 cut(s) 313, 596, 976
Psp6I CCWGG 2 cut(s) 655, 679
PspEI GGTNACC 1 cut(s) 840
PspFI CCCAGC 1 cut(s) 432
PspGI CCWGG 2 cut(s) 655, 679
PspN4I GGNNCC 6 cut(s) 216, 314, 665, 966, 978, 1155
PspPI GGNCC 6 cut(s) 215, 313, 596, 663, 976, 1313
PspPPI RGGWCCY 3 cut(s) 313, 596, 976
PspXI VCTCGAGB 1 cut(s) 870
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 509
RsaI GTAC 2 cut(s) 464, 1282
RsaNI GTAC 2 cut(s) 463, 1281
RseI CAYNNNNRTG 4 cut(s) 487, 529, 658, 889
SaqAI TTAA 1 cut(s) 1302
SatI GCNGC 4 cut(s) 196, 387, 390, 580
Sau3AI GATC 3 cut(s) 99, 512, 949
Sau96I GGNCC 6 cut(s) 215, 313, 596, 663, 976, 1313
SchI GAGTC 1 cut(s) 784
ScrFI CCNGG 2 cut(s) 657, 681
SduI GDGCHC 1 cut(s) 191
SfaNI GCATC 2 cut(s) 813, 1213
SfcI CTRYAG 1 cut(s) 387
Sfr274I CTCGAG 1 cut(s) 870
SinI GGWCC 5 cut(s) 215, 313, 596, 663, 976
SlaI CTCGAG 1 cut(s) 870
SmiMI CAYNNNNRTG 4 cut(s) 487, 529, 658, 889
SmlI CTYRAG 2 cut(s) 870, 1070
SmoI CTYRAG 2 cut(s) 870, 1070
Sse9I AATT 4 cut(s) 414, 1106, 1276, 1341
SseBI AGGCCT 1 cut(s) 114
SsiI CCGC 4 cut(s) 195, 285, 579, 838
SspI AATATT 1 cut(s) 1018
SspMI CTAG 5 cut(s) 317, 485, 528, 1143, 1196
StuI AGGCCT 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 655, 679
StyI CCWWGG 1 cut(s) 754
TaaI ACNGT 3 cut(s) 501, 564, 831
TaiI ACGT 4 cut(s) 240, 468, 703, 907
TaqI TCGA 2 cut(s) 16, 871
TasI AATT 4 cut(s) 414, 1106, 1276, 1341
TatI WGTACW 1 cut(s) 1280
TauI GCSGC 2 cut(s) 198, 582
TfiI GAWTC 1 cut(s) 1091
Tru1I TTAA 1 cut(s) 1302
Tru9I TTAA 1 cut(s) 1302
TscAI CASTG 7 cut(s) 67, 196, 205, 522, 836, 891, 1274
TseFI GTSAC 5 cut(s) 65, 302, 359, 1262, 1352
TseI GCWGC 2 cut(s) 386, 389
Tsp45I GTSAC 5 cut(s) 65, 302, 359, 1262, 1352
TspDTI ATGAA 4 cut(s) 256, 663, 870, 1282
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 7 cut(s) 67, 196, 205, 522, 836, 891, 1274
VneI GTGCAC 1 cut(s) 187
VpaK11BI GGWCC 5 cut(s) 215, 313, 596, 663, 976
XceI RCATGY 1 cut(s) 894
XcmI CCANNNNNNNNNTGG 2 cut(s) 59, 761
XhoI CTCGAG 1 cut(s) 870
XspI CTAG 5 cut(s) 317, 485, 528, 1143, 1196
Zsp2I ATGCAT 2 cut(s) 6, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.