RchiOBHm_Chr2g0093361
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003988
Physical Location & Seq
Reverse (-)
6656374 .. 6657334
961 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46841

Sequence Viewer

Length: 672 bp
ATGTTTCCTGTTACATTGATTGGAATTTTCTTCATGTTGAATGCCTTGATTTGGGAGGAGAAATCCTGTGGTTCAGTGCCACTGGGGACAATGTTTACCCTGGTACTCTTGTGGTCTGGAATATCGGCGCCATTGGTTTTCTTAGGCAGCTACTTTGGCTTCAAAATGTCACCAATTGATGATCCTATCAAAACTAATGAAATTCCAAGACAGATTCCTGAGCGACCATGGCACAACATAAAGCGGCTCTTGTCTATGCTATTTGGAAGCATTCTACCATTTAATGTAGTGCTCACTGAGTTCCTCTCTATCTGGACGTCTATATGGCCAGACCAATACTCTTTCAGGTTCCTCTTCATGGCCTTTGTTATACTTCTACTCACTTGTTCCGAGATGGCTATTCTACTCTGCTACTTCCAACTTCGCAGCGAAGATTACAATTGGTGGTGGAGAGCATACCTGACTATTGGCTCTTCTGGTCTGTACATGTTTTCATACTCCATATTCTATTTCTTCACCAACATGGAGATTGAAAAGTTTGTTTCTGTCATCCTTTATTTCGGGTACATGTTAATGGGATCCTTTGCATTCTTTGTCCTAACGGGGACAATAGGCTTCGGTGCGTGCTTCTGGTTTGTTAGGAAGATCTATTCTTCTGTGAAGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

223

Amino Acids

26.12

Weight (kDa)

6.57

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 1 - 180 4.6e-61 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 127
AciI CCGC 1 cut(s) 244
AclWI GGATC 3 cut(s) 176, 573, 586
AcoI YGGCCR 1 cut(s) 326
AcsI RAATTY 2 cut(s) 24, 201
AcyI GRCGYC 2 cut(s) 128, 317
AfaI GTAC 3 cut(s) 105, 485, 566
AfiI CCNNNNNNNGG 2 cut(s) 51, 358
AflIII ACRYGT 2 cut(s) 486, 567
AgsI TTSAA 3 cut(s) 40, 163, 533
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 1 cut(s) 150
AluI AGCT 1 cut(s) 150
Alw21I GWGCWC 1 cut(s) 294
AlwI GGATC 3 cut(s) 176, 573, 586
AoxI GGCC 2 cut(s) 326, 360
ApeKI GCWGC 2 cut(s) 147, 426
ApoI RAATTY 2 cut(s) 24, 201
AspLEI GCGC 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 162, 508
BaeI ACNNNNGTAYC 2 cut(s) 95, 128
BalI TGGCCA 1 cut(s) 328
BamHI GGATCC 1 cut(s) 578
BanI GGYRCC 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 2 cut(s) 159, 438
BccI CCATC 1 cut(s) 388
BciT130I CCWGG 1 cut(s) 101
BfoI RGCGCY 1 cut(s) 131
BglII AGATCT 1 cut(s) 645
BisI GCNGC 3 cut(s) 148, 245, 427
BlsI GCNGC 3 cut(s) 149, 246, 428
Bme1390I CCNGG 1 cut(s) 101
BmiI GGNNCC 3 cut(s) 129, 350, 580
BmrFI CCNGG 1 cut(s) 101
BmrI ACTGGG 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 92
BplI GAGNNNNNCTC 2 cut(s) 290, 322
Bpu10I CCTNAGC 1 cut(s) 219
BsaHI GRCGYC 2 cut(s) 128, 317
BsaJI CCNNGG 2 cut(s) 99, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 358
Bse1I ACTGG 1 cut(s) 87
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 2 cut(s) 99, 227
BseGI GGATG 1 cut(s) 549
BseLI CCNNNNNNNGG 2 cut(s) 51, 358
BseMII CTCAG 2 cut(s) 210, 288
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 159, 438
BshFI GGCC 2 cut(s) 328, 362
BshNI GGYRCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 294
BslFI GGGAC 2 cut(s) 100, 619
BslI CCNNNNNNNGG 2 cut(s) 51, 358
BsmFI GGGAC 2 cut(s) 100, 619
BsmI GAATGC 3 cut(s) 46, 270, 587
BsnI GGCC 2 cut(s) 328, 362
Bsp1286I GDGCHC 1 cut(s) 294
Bsp1407I TGTACA 1 cut(s) 483
Bsp143I GATC 3 cut(s) 181, 578, 645
Bsp19I CCATGG 1 cut(s) 227
BspACI CCGC 1 cut(s) 244
BspANI GGCC 2 cut(s) 328, 362
BspCNI CTCAG 2 cut(s) 211, 289
BspLI GGNNCC 3 cut(s) 129, 350, 580
BspPI GGATC 3 cut(s) 176, 573, 586
BspQI GCTCTTC 1 cut(s) 478
BspT107I GGYRCC 1 cut(s) 127
BsrGI TGTACA 1 cut(s) 483
BsrI ACTGG 1 cut(s) 87
BssECI CCNNGG 2 cut(s) 99, 227
BssMI GATC 3 cut(s) 181, 578, 645
BssNI GRCGYC 2 cut(s) 128, 317
BssT1I CCWWGG 1 cut(s) 227
Bst2UI CCWGG 1 cut(s) 101
Bst6I CTCTTC 2 cut(s) 359, 478
BstACI GRCGYC 2 cut(s) 128, 317
BstAUI TGTACA 1 cut(s) 483
BstC8I GCNNGC 1 cut(s) 625
BstDEI CTNAG 3 cut(s) 142, 219, 297
BstDSI CCRYGG 1 cut(s) 227
BstF5I GGATG 1 cut(s) 549
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 3 cut(s) 184, 581, 648
BstMBI GATC 3 cut(s) 181, 578, 645
BstMWI GCNNNNNNNGC 2 cut(s) 156, 229
BstNI CCWGG 1 cut(s) 101
BstNSI RCATGY 2 cut(s) 490, 571
BstSCI CCNGG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 159, 438
BstX2I RGATCY 2 cut(s) 578, 645
BstYI RGATCY 2 cut(s) 578, 645
BsuRI GGCC 2 cut(s) 328, 362
BtgI CCRYGG 1 cut(s) 227
BtsCI GGATG 1 cut(s) 549
BtsIMutI CAGTG 3 cut(s) 80, 81, 294
Cac8I GCNNGC 1 cut(s) 625
CfoI GCGC 1 cut(s) 130
Csp6I GTAC 3 cut(s) 104, 484, 565
CviAII CATG 6 cut(s) 34, 228, 358, 487, 523, 568
CviJI RGCY 8 cut(s) 150, 159, 247, 328, 362, 398, 471, 615
CviKI_1 RGCY 8 cut(s) 150, 159, 247, 328, 362, 398, 471, 615
CviQI GTAC 3 cut(s) 104, 484, 565
DdeI CTNAG 3 cut(s) 142, 219, 297
DinI GGCGCC 1 cut(s) 129
DpnI GATC 3 cut(s) 183, 580, 647
DpnII GATC 3 cut(s) 181, 578, 645
EaeI YGGCCR 1 cut(s) 326
Eam1104I CTCTTC 2 cut(s) 359, 478
EarI CTCTTC 2 cut(s) 359, 478
Eco130I CCWWGG 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 99
EcoT14I CCWWGG 1 cut(s) 227
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 6 cut(s) 37, 231, 361, 490, 526, 571
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 2 cut(s) 100, 619
FatI CATG 6 cut(s) 33, 227, 357, 486, 522, 567
Fnu4HI GCNGC 3 cut(s) 148, 245, 427
FokI GGATG 1 cut(s) 536
Fsp4HI GCNGC 3 cut(s) 148, 245, 427
GlaI GCGC 1 cut(s) 129
GluI GCNGC 3 cut(s) 148, 245, 427
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 328, 362
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 2 cut(s) 128, 317
Hin1II CATG 6 cut(s) 37, 231, 361, 490, 526, 571
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 1 cut(s) 214
HphI GGTGA 2 cut(s) 162, 508
Hpy166II GTNNAC 1 cut(s) 96
Hpy188I TCNGA 1 cut(s) 391
Hpy188III TCNNGA 3 cut(s) 117, 218, 313
Hpy8I GTNNAC 1 cut(s) 96
HpyCH4IV ACGT 1 cut(s) 317
HpyCH4V TGCA 1 cut(s) 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 229
HpyF3I CTNAG 3 cut(s) 142, 219, 297
HpySE526I ACGT 1 cut(s) 317
Hsp92I GRCGYC 2 cut(s) 128, 317
Hsp92II CATG 6 cut(s) 37, 231, 361, 490, 526, 571
HspAI GCGC 1 cut(s) 128
KasI GGCGCC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 181, 578, 645
LguI GCTCTTC 1 cut(s) 478
Lsp1109I GCAGC 2 cut(s) 159, 438
MaeII ACGT 1 cut(s) 317
MaeIII GTNAC 2 cut(s) 10, 168
MalI GATC 3 cut(s) 183, 580, 647
MboI GATC 3 cut(s) 181, 578, 645
MboII GAAGA 7 cut(s) 22, 346, 443, 465, 505, 645, 655
MfeI CAATTG 2 cut(s) 174, 439
MflI RGATCY 2 cut(s) 578, 645
MhlI GDGCHC 1 cut(s) 294
MlsI TGGCCA 1 cut(s) 328
MluCI AATT 4 cut(s) 24, 174, 201, 439
MluNI TGGCCA 1 cut(s) 328
Mly113I GGCGCC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 442
MnlI CCTC 3 cut(s) 49, 314, 362
Mox20I TGGCCA 1 cut(s) 328
MscI TGGCCA 1 cut(s) 328
MseI TTAA 2 cut(s) 282, 572
MslI CAYNNNNRTG 2 cut(s) 521, 572
Msp20I TGGCCA 1 cut(s) 328
MspR9I CCNGG 1 cut(s) 101
MunI CAATTG 2 cut(s) 174, 439
Mva1269I GAATGC 3 cut(s) 46, 270, 587
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 156, 229
NarI GGCGCC 1 cut(s) 128
NcoI CCATGG 1 cut(s) 227
NdeII GATC 3 cut(s) 181, 578, 645
NlaIII CATG 6 cut(s) 37, 231, 361, 490, 526, 571
NlaIV GGNNCC 3 cut(s) 129, 350, 580
NmuCI GTSAC 1 cut(s) 168
NspI RCATGY 2 cut(s) 490, 571
PciI ACATGT 2 cut(s) 486, 567
PciSI GCTCTTC 1 cut(s) 478
PctI GAATGC 3 cut(s) 46, 270, 587
PfeI GAWTC 1 cut(s) 214
PkrI GCNGC 3 cut(s) 149, 246, 428
PluTI GGCGCC 1 cut(s) 131
PscI ACATGT 2 cut(s) 486, 567
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 3 cut(s) 129, 350, 580
PsuI RGATCY 2 cut(s) 578, 645
RsaI GTAC 3 cut(s) 105, 485, 566
RsaNI GTAC 3 cut(s) 104, 484, 565
RseI CAYNNNNRTG 2 cut(s) 521, 572
SapI GCTCTTC 1 cut(s) 478
SaqAI TTAA 2 cut(s) 282, 572
SatI GCNGC 3 cut(s) 148, 245, 427
Sau3AI GATC 3 cut(s) 181, 578, 645
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 1 cut(s) 294
SetI ASST 4 cut(s) 152, 320, 350, 462
SfoI GGCGCC 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 521, 572
Sse9I AATT 4 cut(s) 24, 174, 201, 439
SsiI CCGC 1 cut(s) 244
SspDI GGCGCC 1 cut(s) 127
StyD4I CCNGG 1 cut(s) 99
StyI CCWWGG 1 cut(s) 227
TaiI ACGT 1 cut(s) 320
TasI AATT 4 cut(s) 24, 174, 201, 439
TatI WGTACW 1 cut(s) 483
TauI GCSGC 1 cut(s) 247
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 2 cut(s) 282, 572
Tru9I TTAA 2 cut(s) 282, 572
TscAI CASTG 3 cut(s) 81, 87, 301
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 2 cut(s) 147, 426
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 4 cut(s) 22, 213, 346, 483
TspRI CASTG 3 cut(s) 81, 87, 301
XapI RAATTY 2 cut(s) 24, 201
XceI RCATGY 2 cut(s) 490, 571
ZraI GACGTC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.