Rw2G006530
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003988
Physical Location & Seq
Forward (+)
6298240 .. 6300863
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006530.1

Sequence Viewer

Length: 1902 bp
ATGAGTAGCTTCTTCTTCTTCTTGGTCTTCGTCCTTCTCCTCTCTCTCTTGCCTTGGTCTCACTCATTCCATGGCACTTTACCCCGAAACAAGGGAGATACACTTGAAGTAAAGGTTAATCAGCTTTCATCAATCAGAACACAGCTTCCATATGACTACTATTTCCTCAGCTACTGCAAACCTCCAGTAATTATCAACAGTTCCTTCAGCCTTGGACAAGTTCTTGGAGGTGACCGCATCAAGAATTCGGTCTATTCTTTCAAGATGCGAGAGGCACAGTATTGCAAAGTTGCATGCCGTGTAAAACTGGATGCTAATTCTGCGAAAAGTTTTAAGGACAAGATCGATGGTGAATATCAAGTTAACATGATTCTTGATGATCTCCCTGTTGCAGTTGCACTCGAAATGAGAAACTCTCGAGATGAGGGTCGATCAAAGATATATAAGCTTGGGTTCATGGTTGGGATTAAGGGACGTTATGCTGGAGAAAAAGAAGAAAGACATTTCATTCACAACCACCTGAGTTTTAAAGTCATGTATCATGAGGATCCAGAGACTGATTATGCTCACATTGTCGGTTTTGAGGTCACGCCGTACAGTATTAATCACGAGTACAAGGAATGGAATGGTGAGAAGACTAAGCTTGTCACATGCCAGCCGAGCACCAATACTTACATTGAAGGTAGCAATGGCCCACAAGGGATTCATACTGATGAAGAAATTGTTTTCACATATGATGTATCTTTTGAGTCCAGTGATATCAAGTGGGAATCGCGTTGGGACTCATACCTCTACCTAGGTGATGATCCAATTCATTGGTTTGCTATGGTCAATTCATTGATAACTGTGCTCTTCCTTTCCGGAGTGGTAGCCATTGTTGTGATGAGAACTCTTTTCAAGGATATTACCAACTACAATCAGTTGGAGAATCAAGTTGAAGAAACAGGATGGAAACTGGTCCATGGAGATGTCTTCAGGCCACCCATCAATGCTAATATATTGTGTGTTTTTGTTGCGAATGGTGTCCAAGTCTTTGGTACAGTGCTTTTCACAATAACTCTTGCATTGCTGGGTTTCCTTTCACCTTCGAACCAAGGTGGGGTGATTAAAGCAATGGTTCTTTTATGGATGTTCATGGCCTTATTTGGGGGATACTACTCGGCGCACTTGTACAAGATGTTTAAAGGCATTCAGTGGAAGAAGATTGCATTAAAGACAGCCTTTATGTTCCCTGCTACATTTTTCGGAATATTTTTCATGTTGAATGTCTTCATTTGGTGGGAGGAGTCTTTTGCTGTAGTGCCGTTTGGTACAATGTTTTCAATGCTGCTCTTGTGGTTTGGGATATCAGTGCCATTAGTATTTGTAGGCAGTTACTTGGGGTTCAAACAGCAAGCAATTGAGGATCCAGTCAATACCAATGAAATCGCAAGACAAATACCAGTGCGAGCATGGTACAACATAAAGATGGTCTTGTCTGTGCTCATCGGAGGCATTCTACAGTTCGGAGTAGTGTTCGTTGAGATCTTCTTTATGTGGACATCCATATGGATGAATAAGTTCTACTACTCTTTCGGGTTCCTCTTAATAGTCTTTGTCATCCTTCTAGTCACTTGTTGTGAGATAGCAATTCTACTCTGCTACTTCCAACTTCGCAGCGAAGATTACCATTGGTGGTGGAGAGCATACTTGACTGCCGGCTCCTCTGCTCTGTACATGTTTGCATACTCCATTTTCCTTTTCTTCACCAGGCTGTGGATTGAAAAGTTTGTTTCTGTCATCCTTTATTTTGGGTACATGTTAATTGCATCCTTTGCGTTCTTTGTCTTGACTGGGACAATCGGCTTCTATGCATGCTTCTGGTTTGTTAGGAAGATCTACTCCTCTGTCAAGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

633

Amino Acids

73.32

Weight (kDa)

7.18

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 50 - 590 1.4e-187 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1755
AccII CGCG 1 cut(s) 775
AccIII TCCGGA 1 cut(s) 860
AciI CCGC 1 cut(s) 235
AclWI GGATC 5 cut(s) 542, 555, 800, 1400, 1413
AcsI RAATTY 1 cut(s) 244
AcuI CTGAAG 2 cut(s) 190, 958
AfaI GTAC 8 cut(s) 596, 614, 1039, 1172, 1312, 1457, 1715, 1796
AfiI CCNNNNNNNGG 4 cut(s) 91, 1099, 1146, 1755
AflIII ACRYGT 2 cut(s) 1716, 1797
AgsI TTSAA 9 cut(s) 107, 262, 680, 898, 938, 1264, 1323, 1387, 1763
AjnI CCWGG 1 cut(s) 1748
AloI GAACNNNNNNTCC 2 cut(s) 130, 162
AluBI AGCT 6 cut(s) 9, 124, 145, 171, 448, 643
AluI AGCT 6 cut(s) 9, 124, 145, 171, 448, 643
Alw21I GWGCWC 3 cut(s) 665, 852, 1485
Alw26I GTCTC 2 cut(s) 63, 548
AlwI GGATC 5 cut(s) 542, 555, 800, 1400, 1413
AlwNI CAGNNNCTG 2 cut(s) 174, 557
Ama87I CYCGRG 1 cut(s) 417
Aor13HI TCCGGA 1 cut(s) 860
AoxI GGCC 3 cut(s) 691, 977, 1137
ApeKI GCWGC 2 cut(s) 1327, 1656
ApoI RAATTY 1 cut(s) 244
AseI ATTAAT 1 cut(s) 603
Asp700I GAANNNNTTC 2 cut(s) 1268, 1559
AspA2I CCTAGG 1 cut(s) 796
AspLEI GCGC 1 cut(s) 1165
AspS9I GGNCC 2 cut(s) 692, 958
AsuHPI GGTGA 7 cut(s) 242, 362, 641, 812, 1074, 1114, 1738
AsuII TTCGAA 1 cut(s) 1088
AvaI CYCGRG 1 cut(s) 417
AvaII GGWCC 1 cut(s) 958
AvrII CCTAGG 1 cut(s) 796
BamHI GGATCC 2 cut(s) 547, 1405
BauI CACGAG 1 cut(s) 608
BbsI GAAGAC 4 cut(s) 19, 641, 964, 1261
Bbv12I GWGCWC 3 cut(s) 665, 852, 1485
BbvCI CCTCAGC 1 cut(s) 167
BbvI GCAGC 2 cut(s) 1314, 1668
BccI CCATC 4 cut(s) 341, 942, 992, 1462
BceAI ACGGC 3 cut(s) 282, 577, 1288
BciT130I CCWGG 1 cut(s) 1750
BciVI GTATCC 1 cut(s) 1145
BcoDI GTCTC 2 cut(s) 63, 548
BfaI CTAG 2 cut(s) 797, 1607
BfmI CTRYAG 2 cut(s) 1296, 1499
BfuI GTATCC 1 cut(s) 1145
BglII AGATCT 2 cut(s) 1524, 1875
BisI GCNGC 2 cut(s) 1328, 1657
BlnI CCTAGG 1 cut(s) 796
BlsI GCNGC 2 cut(s) 1329, 1658
Bme1390I CCNGG 1 cut(s) 1750
Bme18I GGWCC 1 cut(s) 958
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 2 cut(s) 692, 958
BmiI GGNNCC 4 cut(s) 549, 1407, 1580, 1702
BmrFI CCNGG 1 cut(s) 1750
BmrI ACTGGG 1 cut(s) 1842
BmsI GCATC 4 cut(s) 246, 255, 301, 1817
BmuI ACTGGG 1 cut(s) 1842
BpiI GAAGAC 4 cut(s) 19, 641, 964, 1261
BplI GAGNNNNNCTC 2 cut(s) 400, 432
BpmI CTGGAG 2 cut(s) 168, 504
Bpu10I CCTNAGC 1 cut(s) 167
Bpu14I TTCGAA 1 cut(s) 1088
Bsa29I ATCGAT 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 6 cut(s) 53, 70, 211, 796, 961, 1093
BsaWI WCCGGW 1 cut(s) 860
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 1099, 1146, 1755
Bse118I RCCGGY 1 cut(s) 1697
Bse1I ACTGG 7 cut(s) 185, 312, 753, 960, 1409, 1442, 1837
Bse3DI GCAATG 3 cut(s) 694, 1064, 1119
BseAI TCCGGA 1 cut(s) 860
BseBI CCWGG 1 cut(s) 1750
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 6 cut(s) 53, 70, 211, 796, 961, 1093
BseGI GGATG 8 cut(s) 316, 953, 1134, 1541, 1557, 1599, 1779, 1808
BseLI CCNNNNNNNGG 4 cut(s) 91, 1099, 1146, 1755
BseMI GCAATG 3 cut(s) 694, 1064, 1119
BseMII CTCAG 2 cut(s) 181, 512
BseNI ACTGG 7 cut(s) 185, 312, 753, 960, 1409, 1442, 1837
BseRI GAGGAG 4 cut(s) 29, 1298, 1693, 1873
BseXI GCAGC 2 cut(s) 1314, 1668
BseYI CCCAGC 1 cut(s) 1069
Bsh1236I CGCG 1 cut(s) 775
BshFI GGCC 3 cut(s) 693, 979, 1139
BshVI ATCGAT 1 cut(s) 345
BsiHKAI GWGCWC 3 cut(s) 665, 852, 1485
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 2 cut(s) 861, 1698
BslFI GGGAC 3 cut(s) 486, 794, 1849
BslI CCNNNNNNNGG 4 cut(s) 91, 1099, 1146, 1755
BsmAI GTCTC 2 cut(s) 63, 548
BsmFI GGGAC 3 cut(s) 486, 794, 1849
BsmI GAATGC 2 cut(s) 1188, 1494
BsnI GGCC 3 cut(s) 693, 979, 1139
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 1 cut(s) 417
Bsp119I TTCGAA 1 cut(s) 1088
Bsp1286I GDGCHC 3 cut(s) 665, 852, 1485
Bsp13I TCCGGA 1 cut(s) 860
Bsp1407I TGTACA 2 cut(s) 1170, 1713
Bsp143I GATC 8 cut(s) 342, 379, 431, 547, 805, 1405, 1524, 1875
Bsp19I CCATGG 2 cut(s) 70, 961
BspACI CCGC 1 cut(s) 235
BspANI GGCC 3 cut(s) 693, 979, 1139
BspCNI CTCAG 2 cut(s) 180, 513
BspDI ATCGAT 1 cut(s) 345
BspEI TCCGGA 1 cut(s) 860
BspFNI CGCG 1 cut(s) 775
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 4 cut(s) 549, 1407, 1580, 1702
BspPI GGATC 5 cut(s) 542, 555, 800, 1400, 1413
BspQI GCTCTTC 1 cut(s) 857
BspT104I TTCGAA 1 cut(s) 1088
BspTNI GGTCTC 1 cut(s) 63
BsrDI GCAATG 3 cut(s) 694, 1064, 1119
BsrFI RCCGGY 1 cut(s) 1697
BsrGI TGTACA 2 cut(s) 1170, 1713
BsrI ACTGG 7 cut(s) 185, 312, 753, 960, 1409, 1442, 1837
BssAI RCCGGY 1 cut(s) 1697
BssECI CCNNGG 6 cut(s) 53, 70, 211, 796, 961, 1093
BssMI GATC 8 cut(s) 342, 379, 431, 547, 805, 1405, 1524, 1875
BssSI CACGAG 1 cut(s) 608
BssT1I CCWWGG 6 cut(s) 53, 70, 211, 796, 961, 1093
Bst2BI CACGAG 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 1750
Bst4CI ACNGT 6 cut(s) 200, 279, 599, 847, 1042, 1503
Bst6I CTCTTC 1 cut(s) 857
BstAPI GCANNNNNTGC 1 cut(s) 1814
BstAUI TGTACA 2 cut(s) 1170, 1713
BstBI TTCGAA 1 cut(s) 1088
BstC8I GCNNGC 6 cut(s) 295, 656, 1395, 1449, 1699, 1855
BstDEI CTNAG 3 cut(s) 167, 521, 639
BstDSI CCRYGG 2 cut(s) 70, 961
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 8 cut(s) 316, 953, 1134, 1541, 1557, 1599, 1779, 1808
BstFNI CGCG 1 cut(s) 775
BstHHI GCGC 1 cut(s) 1165
BstKTI GATC 8 cut(s) 345, 382, 434, 550, 808, 1408, 1527, 1878
BstMAI GTCTC 2 cut(s) 63, 548
BstMBI GATC 8 cut(s) 342, 379, 431, 547, 805, 1405, 1524, 1875
BstMWI GCNNNNNNNGC 3 cut(s) 320, 660, 1814
BstNI CCWGG 1 cut(s) 1750
BstNSI RCATGY 5 cut(s) 297, 654, 1720, 1801, 1857
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 1748
BstSFI CTRYAG 2 cut(s) 1296, 1499
BstUI CGCG 1 cut(s) 775
BstV1I GCAGC 2 cut(s) 1314, 1668
BstV2I GAAGAC 4 cut(s) 19, 641, 964, 1261
BstX2I RGATCY 4 cut(s) 547, 1405, 1524, 1875
BstXI CCANNNNNNTGG 2 cut(s) 816, 1034
BstYI RGATCY 4 cut(s) 547, 1405, 1524, 1875
Bsu15I ATCGAT 1 cut(s) 345
BsuI GTATCC 1 cut(s) 1145
BsuRI GGCC 3 cut(s) 693, 979, 1139
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 2 cut(s) 70, 961
BtsCI GGATG 8 cut(s) 316, 953, 1134, 1541, 1557, 1599, 1779, 1808
BtsIMutI CAGTG 5 cut(s) 760, 1047, 1199, 1356, 1449
Cac8I GCNNGC 6 cut(s) 295, 656, 1395, 1449, 1699, 1855
CaiI CAGNNNCTG 2 cut(s) 174, 557
CciI TCATGA 1 cut(s) 541
CfoI GCGC 1 cut(s) 1165
Cfr10I RCCGGY 1 cut(s) 1697
Cfr13I GGNCC 2 cut(s) 692, 958
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 8 cut(s) 595, 613, 1038, 1171, 1311, 1456, 1714, 1795
CviQI GTAC 8 cut(s) 595, 613, 1038, 1171, 1311, 1456, 1714, 1795
DdeI CTNAG 3 cut(s) 167, 521, 639
DpnI GATC 8 cut(s) 344, 381, 433, 549, 807, 1407, 1526, 1877
DpnII GATC 8 cut(s) 342, 379, 431, 547, 805, 1405, 1524, 1875
DraI TTTAAA 2 cut(s) 529, 1183
Eam1104I CTCTTC 1 cut(s) 857
EarI CTCTTC 1 cut(s) 857
Eco130I CCWWGG 6 cut(s) 53, 70, 211, 796, 961, 1093
Eco31I GGTCTC 1 cut(s) 63
Eco32I GATATC 2 cut(s) 760, 1347
Eco47I GGWCC 1 cut(s) 958
Eco57I CTGAAG 2 cut(s) 190, 958
Eco88I CYCGRG 1 cut(s) 417
Eco91I GGTNACC 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 1748
EcoRV GATATC 2 cut(s) 760, 1347
EcoT14I CCWWGG 6 cut(s) 53, 70, 211, 796, 961, 1093
EcoT22I ATGCAT 1 cut(s) 1855
ErhI CCWWGG 6 cut(s) 53, 70, 211, 796, 961, 1093
FalI AAGNNNNNCTT 4 cut(s) 1205, 1237, 1457, 1489
FaqI GGGAC 3 cut(s) 486, 794, 1849
FauNDI CATATG 3 cut(s) 151, 733, 1547
Fnu4HI GCNGC 2 cut(s) 1328, 1657
FokI GGATG 8 cut(s) 323, 960, 1141, 1528, 1564, 1586, 1766, 1795
Fsp4HI GCNGC 2 cut(s) 1328, 1657
FspBI CTAG 2 cut(s) 797, 1607
GlaI GCGC 1 cut(s) 1164
GluI GCNGC 2 cut(s) 1328, 1657
GsaI CCCAGC 1 cut(s) 1073
GsuI CTGGAG 2 cut(s) 168, 504
HaeIII GGCC 3 cut(s) 693, 979, 1139
HapII CCGG 2 cut(s) 861, 1698
HhaI GCGC 1 cut(s) 1165
Hin6I GCGC 1 cut(s) 1163
HinP1I GCGC 1 cut(s) 1163
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 446, 641
HinfI GANTC 7 cut(s) 370, 703, 749, 770, 782, 928, 1286
HpaI GTTAAC 1 cut(s) 364
HpaII CCGG 2 cut(s) 861, 1698
HphI GGTGA 7 cut(s) 242, 362, 641, 812, 1074, 1114, 1738
Hpy166II GTNNAC 2 cut(s) 364, 1539
Hpy188I TCNGA 4 cut(s) 137, 1247, 1490, 1508
Hpy8I GTNNAC 2 cut(s) 364, 1539
HpyAV CCTTC 5 cut(s) 44, 214, 674, 1095, 1613
HpyCH4III ACNGT 6 cut(s) 200, 279, 599, 847, 1042, 1503
HpyCH4IV ACGT 1 cut(s) 475
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 660, 1814
HpyF3I CTNAG 3 cut(s) 167, 521, 639
HpySE526I ACGT 1 cut(s) 475
HspAI GCGC 1 cut(s) 1163
Kpn2I TCCGGA 1 cut(s) 860
KroI GCCGGC 1 cut(s) 1697
KroNI GCCGGC 1 cut(s) 1699
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 8 cut(s) 342, 379, 431, 547, 805, 1405, 1524, 1875
LguI GCTCTTC 1 cut(s) 857
LmnI GCTCC 1 cut(s) 1706
Lsp1109I GCAGC 2 cut(s) 1314, 1668
LweI GCATC 4 cut(s) 246, 255, 301, 1817
MaeI CTAG 2 cut(s) 797, 1607
MaeII ACGT 1 cut(s) 475
MaeIII GTNAC 5 cut(s) 230, 586, 646, 1373, 1609
MalI GATC 8 cut(s) 344, 381, 433, 549, 807, 1407, 1526, 1877
MboI GATC 8 cut(s) 342, 379, 431, 547, 805, 1405, 1524, 1875
MfeI CAATTG 1 cut(s) 1398
MflI RGATCY 4 cut(s) 547, 1405, 1524, 1875
MhlI GDGCHC 3 cut(s) 665, 852, 1485
MluCI AATT 9 cut(s) 189, 244, 316, 720, 810, 832, 1398, 1629, 1803
MlyI GAGTC 3 cut(s) 758, 776, 1295
MmeI TCCRAC 2 cut(s) 903, 1672
Mph1103I ATGCAT 1 cut(s) 1855
MroI TCCGGA 1 cut(s) 860
MroNI GCCGGC 1 cut(s) 1697
MroXI GAANNNNTTC 2 cut(s) 1268, 1559
MslI CAYNNNNRTG 6 cut(s) 711, 878, 966, 1466, 1546, 1550
MspI CCGG 2 cut(s) 861, 1698
MspR9I CCNGG 1 cut(s) 1750
MunI CAATTG 1 cut(s) 1398
Mva1269I GAATGC 2 cut(s) 1188, 1494
MvaI CCWGG 1 cut(s) 1750
MvnI CGCG 1 cut(s) 775
MwoI GCNNNNNNNGC 3 cut(s) 320, 660, 1814
NaeI GCCGGC 1 cut(s) 1699
NcoI CCATGG 2 cut(s) 70, 961
NdeI CATATG 3 cut(s) 151, 733, 1547
NdeII GATC 8 cut(s) 342, 379, 431, 547, 805, 1405, 1524, 1875
NgoMIV GCCGGC 1 cut(s) 1697
NlaIV GGNNCC 4 cut(s) 549, 1407, 1580, 1702
NmeAIII GCCGAG 2 cut(s) 684, 1139
NmuCI GTSAC 4 cut(s) 230, 586, 646, 1609
NsiI ATGCAT 1 cut(s) 1855
NspI RCATGY 5 cut(s) 297, 654, 1720, 1801, 1857
NspV TTCGAA 1 cut(s) 1088
PaeI GCATGC 2 cut(s) 297, 1857
PaeR7I CTCGAG 1 cut(s) 417
PagI TCATGA 1 cut(s) 541
PciI ACATGT 2 cut(s) 1716, 1797
PciSI GCTCTTC 1 cut(s) 857
PctI GAATGC 2 cut(s) 1188, 1494
PdiI GCCGGC 1 cut(s) 1699
PdmI GAANNNNTTC 2 cut(s) 1268, 1559
PfeI GAWTC 4 cut(s) 370, 703, 770, 928
PflMI CCANNNNNTGG 1 cut(s) 1755
PkrI GCNGC 2 cut(s) 1329, 1658
PleI GAGTC 3 cut(s) 757, 776, 1294
PpsI GAGTC 3 cut(s) 757, 776, 1294
PscI ACATGT 2 cut(s) 1716, 1797
PshBI ATTAAT 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 1748
PspEI GGTNACC 1 cut(s) 230
PspFI CCCAGC 1 cut(s) 1069
PspGI CCWGG 1 cut(s) 1748
PspN4I GGNNCC 4 cut(s) 549, 1407, 1580, 1702
PspPI GGNCC 2 cut(s) 692, 958
PstNI CAGNNNCTG 2 cut(s) 174, 557
PsuI RGATCY 4 cut(s) 547, 1405, 1524, 1875
RsaI GTAC 8 cut(s) 596, 614, 1039, 1172, 1312, 1457, 1715, 1796
RsaNI GTAC 8 cut(s) 595, 613, 1038, 1171, 1311, 1456, 1714, 1795
RseI CAYNNNNRTG 6 cut(s) 711, 878, 966, 1466, 1546, 1550
SapI GCTCTTC 1 cut(s) 857
SatI GCNGC 2 cut(s) 1328, 1657
Sau3AI GATC 8 cut(s) 342, 379, 431, 547, 805, 1405, 1524, 1875
Sau96I GGNCC 2 cut(s) 692, 958
SchI GAGTC 3 cut(s) 758, 776, 1295
ScrFI CCNGG 1 cut(s) 1750
SduI GDGCHC 3 cut(s) 665, 852, 1485
SfaNI GCATC 4 cut(s) 246, 255, 301, 1817
SfcI CTRYAG 2 cut(s) 1296, 1499
Sfr274I CTCGAG 1 cut(s) 417
SfuI TTCGAA 1 cut(s) 1088
SinI GGWCC 1 cut(s) 958
SlaI CTCGAG 1 cut(s) 417
SmiMI CAYNNNNRTG 6 cut(s) 711, 878, 966, 1466, 1546, 1550
SmlI CTYRAG 1 cut(s) 417
SmoI CTYRAG 1 cut(s) 417
SphI GCATGC 2 cut(s) 297, 1857
Sse9I AATT 9 cut(s) 189, 244, 316, 720, 810, 832, 1398, 1629, 1803
SsiI CCGC 1 cut(s) 235
SspI AATATT 1 cut(s) 1251
SspMI CTAG 2 cut(s) 797, 1607
StyD4I CCNGG 1 cut(s) 1748
StyI CCWWGG 6 cut(s) 53, 70, 211, 796, 961, 1093
TaaI ACNGT 6 cut(s) 200, 279, 599, 847, 1042, 1503
TaiI ACGT 1 cut(s) 478
TaqI TCGA 5 cut(s) 345, 402, 418, 430, 1088
TaqII GACCGA 1 cut(s) 238
TasI AATT 9 cut(s) 189, 244, 316, 720, 810, 832, 1398, 1629, 1803
TatI WGTACW 3 cut(s) 612, 1170, 1713
TfiI GAWTC 4 cut(s) 370, 703, 770, 928
TscAI CASTG 5 cut(s) 760, 1047, 1199, 1356, 1449
TseFI GTSAC 4 cut(s) 230, 586, 646, 1609
TseI GCWGC 2 cut(s) 1327, 1656
Tsp45I GTSAC 4 cut(s) 230, 586, 646, 1609
TspRI CASTG 5 cut(s) 760, 1047, 1199, 1356, 1449
Van91I CCANNNNNTGG 1 cut(s) 1755
VpaK11BI GGWCC 1 cut(s) 958
VspI ATTAAT 1 cut(s) 603
XapI RAATTY 1 cut(s) 244
XceI RCATGY 5 cut(s) 297, 654, 1720, 1801, 1857
XcmI CCANNNNNNNNNTGG 1 cut(s) 1449
XhoI CTCGAG 1 cut(s) 417
XmaJI CCTAGG 1 cut(s) 796
XmnI GAANNNNTTC 2 cut(s) 1268, 1559
XspI CTAG 2 cut(s) 797, 1607
Zsp2I ATGCAT 1 cut(s) 1855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.