Rh2BG082500
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003988
Physical Location & Seq
Reverse (-)
6391250 .. 6391751
502 bp
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UTR
Exon/CDS
Intron
Rh2BG082500.1

Sequence Viewer

Length: 315 bp
ATGGGCAGCCTCTTCTTCTTCATCCTTCTACTTTCACTTGTTCCTCGGTCTCACTCTTTCTATGGCGATGTAGCTCGGGATTTTAAGAACAAGGAAGATACACTGGAAGTAAAGGTTAATAAGCTATCATCCATCAAGACACTGCTTCCCTATGACTACTATTCCCTTGGTTACTGCAAGCCTCCAGTAATCACCAATAGTGCTTTAAGCCTTGGAGAAGTTCTTCGAGGTGACCGCATCAAGAATTCAATCTATACTGTGAGTGTGCACTTCCTAACCGGACGCTTGGTTTCCAAGCTTGTGACTTGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

104

Amino Acids

11.71

Weight (kDa)

9.24

Isoelectric Point (pI)

21.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 50 - 89 5.7e-07 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 235
AcsI RAATTY 1 cut(s) 244
AgsI TTSAA 1 cut(s) 249
AluBI AGCT 3 cut(s) 74, 124, 298
AluI AGCT 3 cut(s) 74, 124, 298
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 54
Alw44I GTGCAC 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 75
ApaLI GTGCAC 1 cut(s) 266
ApeKI GCWGC 1 cut(s) 6
ApoI RAATTY 1 cut(s) 244
Asp700I GAANNNNTTC 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 184, 242
AvaI CYCGRG 1 cut(s) 75
BaeGI GKGCMC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 18
BccI CCATC 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 54
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
BmeT110I CYCGRG 1 cut(s) 75
BmsI GCATC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 44, 166, 211
BsaWI WCCGGW 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 108, 185
BseDI CCNNGG 3 cut(s) 44, 166, 211
BseGI GGATG 2 cut(s) 21, 128
BseNI ACTGG 2 cut(s) 108, 185
BseSI GKGCMC 1 cut(s) 270
BseXI GCAGC 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 270
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 1 cut(s) 279
BsmAI GTCTC 1 cut(s) 54
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 270
BspACI CCGC 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 54
BsrI ACTGG 2 cut(s) 108, 185
BssECI CCNNGG 3 cut(s) 44, 166, 211
BssT1I CCWWGG 2 cut(s) 166, 211
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 179
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 2 cut(s) 21, 128
BstMAI GTCTC 1 cut(s) 54
BstPI GGTNACC 1 cut(s) 230
BstSLI GKGCMC 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 18
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 2 cut(s) 21, 128
BtsI GCAGTG 1 cut(s) 140
BtsIMutI CAGTG 2 cut(s) 101, 140
Cac8I GCNNGC 1 cut(s) 179
CseI GACGC 1 cut(s) 291
CviJI RGCY 6 cut(s) 9, 74, 124, 181, 210, 298
CviKI_1 RGCY 6 cut(s) 9, 74, 124, 181, 210, 298
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 166, 211
Eco31I GGTCTC 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 75
Eco91I GGTNACC 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 166, 211
ErhI CCWWGG 2 cut(s) 166, 211
FaiI YATR 3 cut(s) 63, 153, 255
Fnu4HI GCNGC 1 cut(s) 7
FokI GGATG 2 cut(s) 8, 115
Fsp4HI GCNGC 1 cut(s) 7
GluI GCNGC 1 cut(s) 7
GsuI CTGGAG 1 cut(s) 168
HapII CCGG 1 cut(s) 279
HgaI GACGC 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 296
HpaII CCGG 1 cut(s) 279
HphI GGTGA 2 cut(s) 184, 242
Hpy166II GTNNAC 1 cut(s) 268
Hpy188III TCNNGA 3 cut(s) 77, 136, 241
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 2 cut(s) 177, 268
LpnPI CCDG 3 cut(s) 89, 198, 292
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 246
MaeIII GTNAC 3 cut(s) 170, 230, 301
MboII GAAGA 5 cut(s) 4, 7, 10, 107, 215
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 1 cut(s) 244
MnlI CCTC 4 cut(s) 20, 54, 192, 221
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 4 cut(s) 84, 117, 206, 313
MspI CCGG 1 cut(s) 279
NmuCI GTSAC 2 cut(s) 230, 301
PdmI GAANNNNTTC 1 cut(s) 222
PkrI GCNGC 1 cut(s) 8
PspEI GGTNACC 1 cut(s) 230
SaqAI TTAA 4 cut(s) 84, 117, 206, 313
SatI GCNGC 1 cut(s) 7
SduI GDGCHC 1 cut(s) 270
SetI ASST 5 cut(s) 76, 117, 126, 232, 300
SfaNI GCATC 1 cut(s) 246
Sse9I AATT 1 cut(s) 244
SsiI CCGC 1 cut(s) 235
StyI CCWWGG 2 cut(s) 166, 211
TaaI ACNGT 1 cut(s) 259
TaqI TCGA 1 cut(s) 226
TaqII GACCGA 1 cut(s) 36
TasI AATT 1 cut(s) 244
Tru1I TTAA 4 cut(s) 84, 117, 206, 313
Tru9I TTAA 4 cut(s) 84, 117, 206, 313
TscAI CASTG 2 cut(s) 108, 147
TseFI GTSAC 2 cut(s) 230, 301
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 2 cut(s) 230, 301
TspDTI ATGAA 1 cut(s) 10
TspRI CASTG 2 cut(s) 108, 147
VneI GTGCAC 1 cut(s) 266
XapI RAATTY 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.