RchiOBHm_Chr2g0093371
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003988
Physical Location & Seq
Reverse (-)
6657336 .. 6659341
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46842

Sequence Viewer

Length: 1296 bp
ATGGGCATCCTCTTCTTCTTCATCCTTCTACTTTCACTTGTTCCTCGGTCTCACTCTTTCTATGGCGATGTAGCTCGGGATTTTAAGAACAAGGAAGATACACTGGAAGTAAAGGTTAATAAGCTATCATCCATCAAGACACTGCTTCCCTATGACTACTATTCCCTTGGTTACTGCAAGCCTCCAGTAATCACCAATAGTGCCTTAAGCCTTGGAGAAGTTCTTCGAGGTGACCGCATCAAGAATTCAATCTATACTCTTAAGATGAGAGAGGCAGAATACTGCAAAGTCGCATGCCGTGTGAAACTGGATGCTAATTCTGCCAAAAAGTTTAGGGAGAAAATCGATGATCAATATGTAATTAATATGATTCTTGATAATCTTCCTGCTGCAATTACATTCGAAGCGAGTGACTGGAATGGGAATAAATCAAAGATACCTGTAACTGCACTCCCAGTTGGGCGGAAGGGATATGCCACAGAAGTGAGTTTTGTTCATCTTCTAATGTTATTTGATTTGAAGAATTGTTTTTGCTTCTTGTTAACATCTAAATCGCTGGCACAGGGCAGTGAGGAAAGACACTTCATTTACAATCACCTGAGATTCAACGTCATGTACCATGAGGATCCGAAGACCAAATTTTCTCGCATTGTTGGTTTTGAGATTACACCATACAGTGTTAATCATGAGTACAAGAAGTGGGAAAATGAGAAAACTGAGTTGGTCACCTGCACGACCAATTCTGACCTTTTAATTAGCAATTTACCGCAAGAGATTGATACTGATAAAGAAGTTGTCTTTACATATGACGTATTGTTCGAGTCCAGTGATGTCAAGTGGGAATCGCGGTGGGACTCATACCTCTCCAGGGGTACTGATAATCAAATCCATTGGTTATCTATGTTCAACTCATTGTGGACTCTGCTCCTTCTTTCTGGAGTAATAGCCATGGTTGTGGTGCGAACTCTTTACAAGGATATCAACAGCTACAATCAGTTGGAGACCCAAGATGAGGCTCAGGAACTAACAGGATGGAAACTAGTCCATGCAGATGTTTTCAGGCCACCCCCCAACGCTAATTTATTGTGTGTTTGTGTTGGGAATGGTGTCCAATTCTTTGGGACGATGCTCATCACGATCATATCTGGTCTGCTTGGTTTCCTTTCACCTTCGAACAGAGGTGGGTTGATCACAGCAATGGTTCTTTCGTGGATGTTCATGGGCTTATTTGGGGATACTACTCGGCGCACTTGTACAAGATGTTCAAAGGCACGCAATGGAAGAAGATTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

431

Amino Acids

49.34

Weight (kDa)

8.39

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 50 - 411 4.1e-99 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 737
Acc36I ACCTGC 1 cut(s) 737
AccII CGCG 1 cut(s) 847
AciI CCGC 4 cut(s) 235, 463, 767, 847
AclWI GGATC 2 cut(s) 620, 633
AcsI RAATTY 2 cut(s) 244, 638
AfaI GTAC 4 cut(s) 617, 692, 874, 1255
AfiI CCNNNNNNNGG 2 cut(s) 868, 1012
AflII CTTAAG 2 cut(s) 205, 260
AgsI TTSAA 5 cut(s) 249, 520, 607, 907, 1266
AhlI ACTAGT 1 cut(s) 1039
AjnI CCWGG 1 cut(s) 866
AjuI GAANNNNNNNTTGG 2 cut(s) 704, 736
AleI CACNNNNGTG 1 cut(s) 482
AluBI AGCT 3 cut(s) 74, 124, 987
AluI AGCT 3 cut(s) 74, 124, 987
Alw26I GTCTC 2 cut(s) 54, 995
AlwI GGATC 2 cut(s) 620, 633
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 1 cut(s) 1061
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 2 cut(s) 244, 638
ArsI GACNNNNNNTTYG 2 cut(s) 800, 832
AseI ATTAAT 1 cut(s) 363
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 1248
AsuHPI GGTGA 5 cut(s) 184, 242, 587, 718, 1158
AsuII TTCGAA 2 cut(s) 402, 1172
AvaI CYCGRG 1 cut(s) 75
BamHI GGATCC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 638
BbvI GCAGC 1 cut(s) 376
BccI CCATC 2 cut(s) 140, 1026
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 1 cut(s) 868
BciVI GTATCC 1 cut(s) 1228
BclI TGATCA 2 cut(s) 349, 1188
BcoDI GTCTC 2 cut(s) 54, 995
BcuI ACTAGT 1 cut(s) 1039
BfaI CTAG 1 cut(s) 1040
BfrI CTTAAG 2 cut(s) 205, 260
BfuAI ACCTGC 1 cut(s) 737
BfuI GTATCC 1 cut(s) 1228
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
Bme1390I CCNGG 1 cut(s) 868
BmeT110I CYCGRG 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 1 cut(s) 868
BmrI ACTGGG 1 cut(s) 449
BmsI GCATC 4 cut(s) 15, 246, 301, 1116
BmuI ACTGGG 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 638
BpmI CTGGAG 3 cut(s) 168, 850, 957
Bpu10I CCTNAGC 1 cut(s) 1017
Bpu14I TTCGAA 2 cut(s) 402, 1172
Bsa29I ATCGAT 1 cut(s) 345
BsaBI GATNNNNATC 1 cut(s) 1130
BsaI GGTCTC 2 cut(s) 54, 995
BsaJI CCNNGG 5 cut(s) 44, 166, 211, 867, 948
Bsc4I CCNNNNNNNGG 2 cut(s) 868, 1012
Bse1I ACTGG 6 cut(s) 108, 185, 312, 419, 455, 825
Bse3DI GCAATG 2 cut(s) 1203, 1282
Bse8I GATNNNNATC 1 cut(s) 1130
BseBI CCWGG 1 cut(s) 868
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 5 cut(s) 44, 166, 211, 867, 948
BseGI GGATG 6 cut(s) 6, 21, 128, 316, 1037, 1218
BseJI GATNNNNATC 1 cut(s) 1130
BseLI CCNNNNNNNGG 2 cut(s) 868, 1012
BseMI GCAATG 2 cut(s) 1203, 1282
BseMII CTCAG 3 cut(s) 590, 708, 1031
BseNI ACTGG 6 cut(s) 108, 185, 312, 419, 455, 825
BseXI GCAGC 1 cut(s) 376
BsgI GTGCAG 2 cut(s) 432, 715
Bsh1236I CGCG 1 cut(s) 847
BshFI GGCC 1 cut(s) 1063
BshVI ATCGAT 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 75
BslFI GGGAC 2 cut(s) 866, 1135
BslI CCNNNNNNNGG 2 cut(s) 868, 1012
BsmAI GTCTC 2 cut(s) 54, 995
BsmFI GGGAC 2 cut(s) 866, 1135
BsnI GGCC 1 cut(s) 1063
Bso31I GGTCTC 2 cut(s) 54, 995
BsoBI CYCGRG 1 cut(s) 75
Bsp119I TTCGAA 2 cut(s) 402, 1172
Bsp1407I TGTACA 1 cut(s) 1253
Bsp143I GATC 4 cut(s) 349, 625, 1137, 1188
Bsp19I CCATGG 1 cut(s) 948
BspACI CCGC 4 cut(s) 235, 463, 767, 847
BspANI GGCC 1 cut(s) 1063
BspCNI CTCAG 3 cut(s) 591, 709, 1030
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 1 cut(s) 847
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 1 cut(s) 627
BspMI ACCTGC 1 cut(s) 737
BspPI GGATC 2 cut(s) 620, 633
BspT104I TTCGAA 2 cut(s) 402, 1172
BspTI CTTAAG 2 cut(s) 205, 260
BspTNI GGTCTC 2 cut(s) 54, 995
BsrDI GCAATG 2 cut(s) 1203, 1282
BsrGI TGTACA 1 cut(s) 1253
BsrI ACTGG 6 cut(s) 108, 185, 312, 419, 455, 825
BssECI CCNNGG 5 cut(s) 44, 166, 211, 867, 948
BssMI GATC 4 cut(s) 349, 625, 1137, 1188
BssT1I CCWWGG 3 cut(s) 166, 211, 948
Bst2UI CCWGG 1 cut(s) 868
Bst4CI ACNGT 1 cut(s) 677
Bst6I CTCTTC 1 cut(s) 17
BstAFI CTTAAG 2 cut(s) 205, 260
BstAUI TGTACA 1 cut(s) 1253
BstBI TTCGAA 2 cut(s) 402, 1172
BstC8I GCNNGC 4 cut(s) 179, 295, 558, 1273
BstDEI CTNAG 3 cut(s) 599, 717, 1017
BstDSI CCRYGG 1 cut(s) 948
BstEII GGTNACC 2 cut(s) 230, 724
BstF5I GGATG 6 cut(s) 6, 21, 128, 316, 1037, 1218
BstFNI CGCG 1 cut(s) 847
BstHHI GCGC 1 cut(s) 1248
BstKTI GATC 4 cut(s) 352, 628, 1140, 1191
BstMAI GTCTC 2 cut(s) 54, 995
BstMBI GATC 4 cut(s) 349, 625, 1137, 1188
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNI CCWGG 1 cut(s) 868
BstNSI RCATGY 1 cut(s) 297
BstPI GGTNACC 2 cut(s) 230, 724
BstSCI CCNGG 1 cut(s) 866
BstUI CGCG 1 cut(s) 847
BstV1I GCAGC 1 cut(s) 376
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 2 cut(s) 955, 1118
BstYI RGATCY 1 cut(s) 625
Bsu15I ATCGAT 1 cut(s) 345
BsuI GTATCC 1 cut(s) 1228
BsuRI GGCC 1 cut(s) 1063
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 1 cut(s) 948
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 6 cut(s) 6, 21, 128, 316, 1037, 1218
BtsI GCAGTG 2 cut(s) 140, 574
BtsIMutI CAGTG 5 cut(s) 101, 140, 574, 682, 832
BveI ACCTGC 1 cut(s) 737
Cac8I GCNNGC 4 cut(s) 179, 295, 558, 1273
CciI TCATGA 1 cut(s) 685
CfoI GCGC 1 cut(s) 1248
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 4 cut(s) 616, 691, 873, 1254
CviAII CATG 7 cut(s) 294, 613, 620, 686, 949, 1046, 1219
CviJI RGCY 9 cut(s) 74, 124, 181, 210, 947, 987, 1016, 1063, 1224
CviKI_1 RGCY 9 cut(s) 74, 124, 181, 210, 947, 987, 1016, 1063, 1224
CviQI GTAC 4 cut(s) 616, 691, 873, 1254
DdeI CTNAG 3 cut(s) 599, 717, 1017
DpnI GATC 4 cut(s) 351, 627, 1139, 1190
DpnII GATC 4 cut(s) 349, 625, 1137, 1188
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
EciI GGCGGA 1 cut(s) 478
Eco130I CCWWGG 3 cut(s) 166, 211, 948
Eco31I GGTCTC 2 cut(s) 54, 995
Eco32I GATATC 1 cut(s) 979
Eco88I CYCGRG 1 cut(s) 75
Eco91I GGTNACC 2 cut(s) 230, 724
EcoO65I GGTNACC 2 cut(s) 230, 724
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 866
EcoRV GATATC 1 cut(s) 979
EcoT14I CCWWGG 3 cut(s) 166, 211, 948
ErhI CCWWGG 3 cut(s) 166, 211, 948
FaeI CATG 7 cut(s) 297, 616, 623, 689, 952, 1049, 1222
FaqI GGGAC 2 cut(s) 866, 1135
FatI CATG 7 cut(s) 293, 612, 619, 685, 948, 1045, 1218
FauNDI CATATG 1 cut(s) 805
FbaI TGATCA 2 cut(s) 349, 1188
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 5 cut(s) 8, 115, 323, 1044, 1225
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 1 cut(s) 1040
GlaI GCGC 1 cut(s) 1247
GluI GCNGC 1 cut(s) 390
GsuI CTGGAG 3 cut(s) 168, 850, 957
HaeIII GGCC 1 cut(s) 1063
HhaI GCGC 1 cut(s) 1248
Hin1II CATG 7 cut(s) 297, 616, 623, 689, 952, 1049, 1222
Hin6I GCGC 1 cut(s) 1246
HinP1I GCGC 1 cut(s) 1246
HincII GTYRAC 1 cut(s) 543
HindII GTYRAC 1 cut(s) 543
HinfI GANTC 6 cut(s) 370, 603, 821, 842, 854, 919
HpaI GTTAAC 1 cut(s) 543
HphI GGTGA 5 cut(s) 184, 242, 587, 718, 1158
Hpy166II GTNNAC 2 cut(s) 543, 918
Hpy188I TCNGA 2 cut(s) 630, 745
Hpy188III TCNNGA 8 cut(s) 77, 136, 241, 374, 686, 936, 1019, 1135
Hpy8I GTNNAC 2 cut(s) 543, 918
HpyAV CCTTC 4 cut(s) 35, 460, 938, 1179
HpyCH4III ACNGT 1 cut(s) 677
HpyCH4IV ACGT 2 cut(s) 609, 810
HpyCH4V TGCA 7 cut(s) 177, 285, 392, 449, 732, 1049, 1291
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 3 cut(s) 599, 717, 1017
HpySE526I ACGT 2 cut(s) 609, 810
Hsp92II CATG 7 cut(s) 297, 616, 623, 689, 952, 1049, 1222
HspAI GCGC 1 cut(s) 1246
Ksp22I TGATCA 2 cut(s) 349, 1188
KspAI GTTAAC 1 cut(s) 543
Kzo9I GATC 4 cut(s) 349, 625, 1137, 1188
LmnI GCTCC 1 cut(s) 930
Lsp1109I GCAGC 1 cut(s) 376
LweI GCATC 4 cut(s) 15, 246, 301, 1116
MaeI CTAG 1 cut(s) 1040
MaeII ACGT 2 cut(s) 609, 810
MaeIII GTNAC 5 cut(s) 170, 230, 410, 442, 724
MalI GATC 4 cut(s) 351, 627, 1139, 1190
MboI GATC 4 cut(s) 349, 625, 1137, 1188
MflI RGATCY 1 cut(s) 625
MlyI GAGTC 3 cut(s) 830, 848, 913
MmeI TCCRAC 1 cut(s) 978
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 9 cut(s) 84, 117, 206, 261, 363, 542, 681, 752, 1294
MslI CAYNNNNRTG 4 cut(s) 482, 953, 1050, 1196
MspCI CTTAAG 2 cut(s) 205, 260
MspR9I CCNGG 1 cut(s) 868
MvaI CCWGG 1 cut(s) 868
MvnI CGCG 1 cut(s) 847
MwoI GCNNNNNNNGC 1 cut(s) 320
NcoI CCATGG 1 cut(s) 948
NdeI CATATG 1 cut(s) 805
NdeII GATC 4 cut(s) 349, 625, 1137, 1188
NlaIII CATG 7 cut(s) 297, 616, 623, 689, 952, 1049, 1222
NlaIV GGNNCC 1 cut(s) 627
NmeAIII GCCGAG 1 cut(s) 1222
NmuCI GTSAC 3 cut(s) 230, 410, 724
NspI RCATGY 1 cut(s) 297
NspV TTCGAA 2 cut(s) 402, 1172
OliI CACNNNNGTG 1 cut(s) 482
PaeI GCATGC 1 cut(s) 297
PagI TCATGA 1 cut(s) 685
PaqCI CACCTGC 1 cut(s) 737
PcsI WCGNNNNNNNCGW 1 cut(s) 816
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 3 cut(s) 370, 603, 842
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 3 cut(s) 829, 848, 913
PpsI GAGTC 3 cut(s) 829, 848, 913
PshBI ATTAAT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 866
PspEI GGTNACC 2 cut(s) 230, 724
PspGI CCWGG 1 cut(s) 866
PspN4I GGNNCC 1 cut(s) 627
PsrI GAACNNNNNNTAC 2 cut(s) 1246, 1278
PsuI RGATCY 1 cut(s) 625
RsaI GTAC 4 cut(s) 617, 692, 874, 1255
RsaNI GTAC 4 cut(s) 616, 691, 873, 1254
RseI CAYNNNNRTG 4 cut(s) 482, 953, 1050, 1196
SaqAI TTAA 9 cut(s) 84, 117, 206, 261, 363, 542, 681, 752, 1294
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 4 cut(s) 349, 625, 1137, 1188
SchI GAGTC 3 cut(s) 830, 848, 913
ScrFI CCNGG 1 cut(s) 868
SfaNI GCATC 4 cut(s) 15, 246, 301, 1116
SfuI TTCGAA 2 cut(s) 402, 1172
SmiMI CAYNNNNRTG 4 cut(s) 482, 953, 1050, 1196
SmlI CTYRAG 2 cut(s) 205, 260
SmoI CTYRAG 2 cut(s) 205, 260
SpeI ACTAGT 1 cut(s) 1039
SphI GCATGC 1 cut(s) 297
SsiI CCGC 4 cut(s) 235, 463, 767, 847
SspMI CTAG 1 cut(s) 1040
StyD4I CCNGG 1 cut(s) 866
StyI CCWWGG 3 cut(s) 166, 211, 948
TaaI ACNGT 1 cut(s) 677
TaiI ACGT 2 cut(s) 612, 813
TaqI TCGA 5 cut(s) 226, 345, 402, 819, 1172
TaqII GACCGA 1 cut(s) 36
TatI WGTACW 2 cut(s) 690, 1253
TfiI GAWTC 3 cut(s) 370, 603, 842
Tru1I TTAA 9 cut(s) 84, 117, 206, 261, 363, 542, 681, 752, 1294
Tru9I TTAA 9 cut(s) 84, 117, 206, 261, 363, 542, 681, 752, 1294
TscAI CASTG 5 cut(s) 108, 147, 574, 682, 832
TseFI GTSAC 3 cut(s) 230, 410, 724
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 3 cut(s) 230, 410, 724
TspDTI ATGAA 4 cut(s) 10, 485, 574, 1207
TspRI CASTG 5 cut(s) 108, 147, 574, 682, 832
Vha464I CTTAAG 2 cut(s) 205, 260
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 2 cut(s) 244, 638
XceI RCATGY 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.