Rh2DG080300
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003988
Physical Location & Seq
Reverse (-)
6266150 .. 6268776
2627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG080300.1

Sequence Viewer

Length: 1932 bp
ATGATGAATAGCTTCTTCTTCTTCTTGGTCTTCGTCCTTCTCCTCTCTCTCTTGCCTTGGTCTCACTCATTCCATGGCACTTTACCCCGGAACAAGGTAACTTTGAAAAACCGGGTCTCAATGGGAGATACACTTGAAGTAAAGGTTAATCAGCTTTCATCAATCAGAACACAGCTTCCATATGACTACTATTTCCTCAGCTACTGCAAACCTCCAGTAATTATCAACAGTTCCTTCAGCCTTGGACAAGTTCTTGGAGGTGACCGCATCAAGAATTCGGTCTATTCTTTCAAGATGCGAGAGGCACAGTATTGCAAAGTTGCATGCCGTGTAAAACTGGATGCTAATTCTGCGAAAAGTTTTAAGGACAAGATCGATGGTGAATATCAAGTTAACATGATTCTTGATGATCTCCCTGTTGCAGTTGCACTCGAAATGAGAAACTCTCGAGATGAGGGTCGATCAAAGATATATAAGCTTGGGTTCATGGTTGGGATTAAGGGACGTTATGCTGGAGAAAAAGAAGAAAGACATTTCATTCACAACCACCTGAGTTTTAAAGTCATGTATCATGAGGATCCAGAGACTGATTATGCTCACATTGTCGGTTTTGAGGTCACGCCGTACAGTATTAATCACGAGTACAAGGAGTGGAATGGTGAGAAGACTAAGCTTGTCACATGCCAGCCGAGCACCAATACTTACATTGAAGGTAGCAATAGCCCACAAGGGATTCATACTGATGAAGAAATTGTTTTCACATATGATGTATCTTTTGAGTCCAGTGATATCAAGTGGGAATCGCGTTGGGACTCATACCTCTACCTAGGTGATGATCCAATTCATTGGTTTGCTATGGTCAATTCATTGATAACTGTGCTCTTCCTTTCCGGAGTGGTAGCCATTGTTGTGATGAGAACTCTTTTCAAGGATATTACCAACTACAATCAGTTGGAGACCCAAGTTGAAGAAACAGGATGGAAACTGGTCCATGGAGATGTCTTCAGGCCACCCATCAATGCTAATATATTGTGTGTTTTTGTTGCGAATGGTGTCCAAGTCTTTGGTACAGTGCTTTTCACAATAACTCTTGCATTGCTGGGTTTCCTTTCACCTTCGAACCAAGGTGGGGTGATTAAAGCAATGGTTCTTTTATGGATGTTCATGGCCTTATTTGGGGGATACTACTCGGCGCACTTGTACAAGATGTTTAAAGGCATTCAGTGGAAGAAGATTGCATTAAAGACAGCCTTTATGTTCCCTGCTACATTTTTCGGAATATTTTTCATGTTGAATGTCTTCATTTGGTGGGAGGAGTCTTTTGCTGTAGTGCCGTTTGGTACAATGTTTTCAATGCTGCTCTTGTGGTTTGGGATATCAGTGCCATTAGTATTTGTAGGCAGTTACTTGGGGTTCAAACAGCAAGCAATTGAGGATCCAGTCAATACCAATGAAATCGCAAGACAAATACCAGTGCGAGCATGGTACAACATAAAGATGGTCTTGTCTGTGCTCATCGGAGGCATTCTACAGTTCGGAGTAGTGTTCGTTGAGTTCTTCTTTATGTGGACATCCATATGGATGAATAAGTTCTACTACTCTTTCGGGTTCCTCTTAATAGTCTTTGTCATCCTTCTAGTCACTTGTTGTGAGATAGCAATTCTACTCTGCTACTTCCAACTTCGCAGCGAAGATTACCATTGGTGGTGGAGAGCATACTTGACTGCCGGCTCCTCTGCTCTGTACATGTTTGCATACTCCATTTTCCTTTTCTTCACCAGGCTGTGGATTGAAAAGTTTGTTTCTGTCATCCTTTATTTTGGGTACATGTTAATTGCATCCTTTGCGTTCTTTGTCTTGACTGGGACAATCGGCTTCTATGCATGCTTCTGGTTTGTTAGGAAGATCTACTCCTCTGTCAAGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

643

Amino Acids

74.56

Weight (kDa)

7.92

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 60 - 600 3.9e-187 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1785
AccII CGCG 1 cut(s) 805
AccIII TCCGGA 1 cut(s) 890
AciI CCGC 1 cut(s) 265
AclWI GGATC 5 cut(s) 572, 585, 830, 1430, 1443
AcsI RAATTY 1 cut(s) 274
AcuI CTGAAG 2 cut(s) 220, 988
AfaI GTAC 8 cut(s) 626, 644, 1069, 1202, 1342, 1487, 1745, 1826
AfiI CCNNNNNNNGG 4 cut(s) 94, 1129, 1176, 1785
AflIII ACRYGT 2 cut(s) 1746, 1827
AjnI CCWGG 1 cut(s) 1778
AloI GAACNNNNNNTCC 2 cut(s) 160, 192
AluBI AGCT 6 cut(s) 12, 154, 175, 201, 478, 673
AluI AGCT 6 cut(s) 12, 154, 175, 201, 478, 673
Alw21I GWGCWC 3 cut(s) 695, 882, 1515
Alw26I GTCTC 4 cut(s) 66, 121, 578, 950
AlwI GGATC 5 cut(s) 572, 585, 830, 1430, 1443
AlwNI CAGNNNCTG 2 cut(s) 204, 587
Ama87I CYCGRG 1 cut(s) 447
Aor13HI TCCGGA 1 cut(s) 890
AoxI GGCC 2 cut(s) 1007, 1167
ApeKI GCWGC 2 cut(s) 1357, 1686
ApoI RAATTY 1 cut(s) 274
AseI ATTAAT 1 cut(s) 633
Asp700I GAANNNNTTC 3 cut(s) 11, 1298, 1589
AspA2I CCTAGG 1 cut(s) 826
AspLEI GCGC 1 cut(s) 1195
AspS9I GGNCC 1 cut(s) 988
AsuC2I CCSGG 2 cut(s) 88, 113
AsuHPI GGTGA 7 cut(s) 272, 392, 671, 842, 1104, 1144, 1768
AsuII TTCGAA 1 cut(s) 1118
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 1 cut(s) 988
AvrII CCTAGG 1 cut(s) 826
BamHI GGATCC 2 cut(s) 577, 1435
BauI CACGAG 1 cut(s) 638
BbsI GAAGAC 4 cut(s) 22, 671, 994, 1291
Bbv12I GWGCWC 3 cut(s) 695, 882, 1515
BbvCI CCTCAGC 1 cut(s) 197
BbvI GCAGC 2 cut(s) 1344, 1698
BccI CCATC 4 cut(s) 371, 972, 1022, 1492
BceAI ACGGC 3 cut(s) 312, 607, 1318
BciT130I CCWGG 1 cut(s) 1780
BciVI GTATCC 1 cut(s) 1175
BcnI CCSGG 2 cut(s) 88, 113
BcoDI GTCTC 4 cut(s) 66, 121, 578, 950
BfaI CTAG 2 cut(s) 827, 1637
BfmI CTRYAG 2 cut(s) 1326, 1529
BfuI GTATCC 1 cut(s) 1175
BglII AGATCT 1 cut(s) 1905
BisI GCNGC 2 cut(s) 1358, 1687
BlnI CCTAGG 1 cut(s) 826
BlsI GCNGC 2 cut(s) 1359, 1688
Bme1390I CCNGG 3 cut(s) 88, 113, 1780
Bme18I GGWCC 1 cut(s) 988
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 988
BmiI GGNNCC 4 cut(s) 579, 1437, 1610, 1732
BmrFI CCNGG 3 cut(s) 88, 113, 1780
BmrI ACTGGG 1 cut(s) 1872
BmsI GCATC 4 cut(s) 276, 285, 331, 1847
BmuI ACTGGG 1 cut(s) 1872
BpiI GAAGAC 4 cut(s) 22, 671, 994, 1291
BplI GAGNNNNNCTC 2 cut(s) 430, 462
BpmI CTGGAG 2 cut(s) 198, 534
Bpu10I CCTNAGC 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 1118
BpuMI CCSGG 2 cut(s) 88, 113
Bsa29I ATCGAT 1 cut(s) 375
BsaI GGTCTC 3 cut(s) 66, 121, 950
BsaJI CCNNGG 7 cut(s) 56, 73, 86, 241, 826, 991, 1123
BsaWI WCCGGW 1 cut(s) 890
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 1129, 1176, 1785
Bse118I RCCGGY 1 cut(s) 1727
Bse1I ACTGG 7 cut(s) 215, 342, 783, 990, 1439, 1472, 1867
Bse3DI GCAATG 2 cut(s) 1094, 1149
BseAI TCCGGA 1 cut(s) 890
BseBI CCWGG 1 cut(s) 1780
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 7 cut(s) 56, 73, 86, 241, 826, 991, 1123
BseGI GGATG 8 cut(s) 346, 983, 1164, 1571, 1587, 1629, 1809, 1838
BseLI CCNNNNNNNGG 4 cut(s) 94, 1129, 1176, 1785
BseMI GCAATG 2 cut(s) 1094, 1149
BseMII CTCAG 2 cut(s) 211, 542
BseNI ACTGG 7 cut(s) 215, 342, 783, 990, 1439, 1472, 1867
BseRI GAGGAG 4 cut(s) 32, 1328, 1723, 1903
BseXI GCAGC 2 cut(s) 1344, 1698
BseYI CCCAGC 1 cut(s) 1099
Bsh1236I CGCG 1 cut(s) 805
BshFI GGCC 2 cut(s) 1009, 1169
BshVI ATCGAT 1 cut(s) 375
BsiHKAI GWGCWC 3 cut(s) 695, 882, 1515
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 4 cut(s) 88, 112, 891, 1728
BslFI GGGAC 3 cut(s) 516, 824, 1879
BslI CCNNNNNNNGG 4 cut(s) 94, 1129, 1176, 1785
BsmAI GTCTC 4 cut(s) 66, 121, 578, 950
BsmFI GGGAC 3 cut(s) 516, 824, 1879
BsmI GAATGC 2 cut(s) 1218, 1524
BsnI GGCC 2 cut(s) 1009, 1169
Bso31I GGTCTC 3 cut(s) 66, 121, 950
BsoBI CYCGRG 1 cut(s) 447
Bsp119I TTCGAA 1 cut(s) 1118
Bsp1286I GDGCHC 3 cut(s) 695, 882, 1515
Bsp13I TCCGGA 1 cut(s) 890
Bsp1407I TGTACA 2 cut(s) 1200, 1743
Bsp143I GATC 7 cut(s) 372, 409, 461, 577, 835, 1435, 1905
Bsp19I CCATGG 2 cut(s) 73, 991
BspACI CCGC 1 cut(s) 265
BspANI GGCC 2 cut(s) 1009, 1169
BspCNI CTCAG 2 cut(s) 210, 543
BspDI ATCGAT 1 cut(s) 375
BspEI TCCGGA 1 cut(s) 890
BspFNI CGCG 1 cut(s) 805
BspHI TCATGA 1 cut(s) 571
BspLI GGNNCC 4 cut(s) 579, 1437, 1610, 1732
BspPI GGATC 5 cut(s) 572, 585, 830, 1430, 1443
BspQI GCTCTTC 1 cut(s) 887
BspT104I TTCGAA 1 cut(s) 1118
BspTNI GGTCTC 3 cut(s) 66, 121, 950
BsrDI GCAATG 2 cut(s) 1094, 1149
BsrFI RCCGGY 1 cut(s) 1727
BsrGI TGTACA 2 cut(s) 1200, 1743
BsrI ACTGG 7 cut(s) 215, 342, 783, 990, 1439, 1472, 1867
BssAI RCCGGY 1 cut(s) 1727
BssECI CCNNGG 7 cut(s) 56, 73, 86, 241, 826, 991, 1123
BssMI GATC 7 cut(s) 372, 409, 461, 577, 835, 1435, 1905
BssSI CACGAG 1 cut(s) 638
BssT1I CCWWGG 6 cut(s) 56, 73, 241, 826, 991, 1123
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 1 cut(s) 1780
Bst4CI ACNGT 6 cut(s) 230, 309, 629, 877, 1072, 1533
Bst6I CTCTTC 1 cut(s) 887
BstAPI GCANNNNNTGC 1 cut(s) 1844
BstAUI TGTACA 2 cut(s) 1200, 1743
BstBI TTCGAA 1 cut(s) 1118
BstC8I GCNNGC 6 cut(s) 325, 686, 1425, 1479, 1729, 1885
BstDEI CTNAG 3 cut(s) 197, 551, 669
BstDSI CCRYGG 2 cut(s) 73, 991
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 8 cut(s) 346, 983, 1164, 1571, 1587, 1629, 1809, 1838
BstFNI CGCG 1 cut(s) 805
BstHHI GCGC 1 cut(s) 1195
BstKTI GATC 7 cut(s) 375, 412, 464, 580, 838, 1438, 1908
BstMAI GTCTC 4 cut(s) 66, 121, 578, 950
BstMBI GATC 7 cut(s) 372, 409, 461, 577, 835, 1435, 1905
BstMWI GCNNNNNNNGC 3 cut(s) 350, 690, 1844
BstNI CCWGG 1 cut(s) 1780
BstNSI RCATGY 5 cut(s) 327, 684, 1750, 1831, 1887
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 86, 111, 1778
BstSFI CTRYAG 2 cut(s) 1326, 1529
BstUI CGCG 1 cut(s) 805
BstV1I GCAGC 2 cut(s) 1344, 1698
BstV2I GAAGAC 4 cut(s) 22, 671, 994, 1291
BstX2I RGATCY 3 cut(s) 577, 1435, 1905
BstXI CCANNNNNNTGG 2 cut(s) 846, 1064
BstYI RGATCY 3 cut(s) 577, 1435, 1905
Bsu15I ATCGAT 1 cut(s) 375
BsuI GTATCC 1 cut(s) 1175
BsuRI GGCC 2 cut(s) 1009, 1169
BsuTUI ATCGAT 1 cut(s) 375
BtgI CCRYGG 2 cut(s) 73, 991
BtsCI GGATG 8 cut(s) 346, 983, 1164, 1571, 1587, 1629, 1809, 1838
BtsIMutI CAGTG 5 cut(s) 790, 1077, 1229, 1386, 1479
Cac8I GCNNGC 6 cut(s) 325, 686, 1425, 1479, 1729, 1885
CaiI CAGNNNCTG 2 cut(s) 204, 587
CciI TCATGA 1 cut(s) 571
CfoI GCGC 1 cut(s) 1195
Cfr10I RCCGGY 1 cut(s) 1727
Cfr13I GGNCC 1 cut(s) 988
ClaI ATCGAT 1 cut(s) 375
Csp6I GTAC 8 cut(s) 625, 643, 1068, 1201, 1341, 1486, 1744, 1825
CviQI GTAC 8 cut(s) 625, 643, 1068, 1201, 1341, 1486, 1744, 1825
DdeI CTNAG 3 cut(s) 197, 551, 669
DpnI GATC 7 cut(s) 374, 411, 463, 579, 837, 1437, 1907
DpnII GATC 7 cut(s) 372, 409, 461, 577, 835, 1435, 1905
DraI TTTAAA 2 cut(s) 559, 1213
Eam1104I CTCTTC 1 cut(s) 887
EarI CTCTTC 1 cut(s) 887
Eco130I CCWWGG 6 cut(s) 56, 73, 241, 826, 991, 1123
Eco31I GGTCTC 3 cut(s) 66, 121, 950
Eco32I GATATC 2 cut(s) 790, 1377
Eco47I GGWCC 1 cut(s) 988
Eco57I CTGAAG 2 cut(s) 220, 988
Eco88I CYCGRG 1 cut(s) 447
Eco91I GGTNACC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 1778
EcoRV GATATC 2 cut(s) 790, 1377
EcoT14I CCWWGG 6 cut(s) 56, 73, 241, 826, 991, 1123
EcoT22I ATGCAT 1 cut(s) 1885
ErhI CCWWGG 6 cut(s) 56, 73, 241, 826, 991, 1123
FalI AAGNNNNNCTT 4 cut(s) 1235, 1267, 1487, 1519
FaqI GGGAC 3 cut(s) 516, 824, 1879
FauNDI CATATG 3 cut(s) 181, 763, 1577
Fnu4HI GCNGC 2 cut(s) 1358, 1687
FokI GGATG 8 cut(s) 353, 990, 1171, 1558, 1594, 1616, 1796, 1825
Fsp4HI GCNGC 2 cut(s) 1358, 1687
FspBI CTAG 2 cut(s) 827, 1637
GlaI GCGC 1 cut(s) 1194
GluI GCNGC 2 cut(s) 1358, 1687
GsaI CCCAGC 1 cut(s) 1103
GsuI CTGGAG 2 cut(s) 198, 534
HaeIII GGCC 2 cut(s) 1009, 1169
HapII CCGG 4 cut(s) 88, 112, 891, 1728
HhaI GCGC 1 cut(s) 1195
Hin6I GCGC 1 cut(s) 1193
HinP1I GCGC 1 cut(s) 1193
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 2 cut(s) 476, 671
HinfI GANTC 6 cut(s) 400, 733, 779, 800, 812, 1316
HpaI GTTAAC 1 cut(s) 394
HpaII CCGG 4 cut(s) 88, 112, 891, 1728
HphI GGTGA 7 cut(s) 272, 392, 671, 842, 1104, 1144, 1768
Hpy166II GTNNAC 2 cut(s) 394, 1569
Hpy188I TCNGA 4 cut(s) 167, 1277, 1520, 1538
Hpy8I GTNNAC 2 cut(s) 394, 1569
HpyAV CCTTC 5 cut(s) 47, 244, 704, 1125, 1643
HpyCH4III ACNGT 6 cut(s) 230, 309, 629, 877, 1072, 1533
HpyCH4IV ACGT 1 cut(s) 505
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 690, 1844
HpyF3I CTNAG 3 cut(s) 197, 551, 669
HpySE526I ACGT 1 cut(s) 505
HspAI GCGC 1 cut(s) 1193
Kpn2I TCCGGA 1 cut(s) 890
KroI GCCGGC 1 cut(s) 1727
KroNI GCCGGC 1 cut(s) 1729
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 7 cut(s) 372, 409, 461, 577, 835, 1435, 1905
LguI GCTCTTC 1 cut(s) 887
LmnI GCTCC 1 cut(s) 1736
Lsp1109I GCAGC 2 cut(s) 1344, 1698
LweI GCATC 4 cut(s) 276, 285, 331, 1847
MaeI CTAG 2 cut(s) 827, 1637
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 6 cut(s) 97, 260, 616, 676, 1403, 1639
MalI GATC 7 cut(s) 374, 411, 463, 579, 837, 1437, 1907
MboI GATC 7 cut(s) 372, 409, 461, 577, 835, 1435, 1905
MfeI CAATTG 1 cut(s) 1428
MflI RGATCY 3 cut(s) 577, 1435, 1905
MhlI GDGCHC 3 cut(s) 695, 882, 1515
MluCI AATT 9 cut(s) 219, 274, 346, 750, 840, 862, 1428, 1659, 1833
MlyI GAGTC 3 cut(s) 788, 806, 1325
MmeI TCCRAC 2 cut(s) 933, 1702
Mph1103I ATGCAT 1 cut(s) 1885
MroI TCCGGA 1 cut(s) 890
MroNI GCCGGC 1 cut(s) 1727
MroXI GAANNNNTTC 3 cut(s) 11, 1298, 1589
MslI CAYNNNNRTG 6 cut(s) 741, 908, 996, 1496, 1576, 1580
MspI CCGG 4 cut(s) 88, 112, 891, 1728
MspR9I CCNGG 3 cut(s) 88, 113, 1780
MunI CAATTG 1 cut(s) 1428
Mva1269I GAATGC 2 cut(s) 1218, 1524
MvaI CCWGG 1 cut(s) 1780
MvnI CGCG 1 cut(s) 805
MwoI GCNNNNNNNGC 3 cut(s) 350, 690, 1844
NaeI GCCGGC 1 cut(s) 1729
NciI CCSGG 2 cut(s) 88, 113
NcoI CCATGG 2 cut(s) 73, 991
NdeI CATATG 3 cut(s) 181, 763, 1577
NdeII GATC 7 cut(s) 372, 409, 461, 577, 835, 1435, 1905
NgoMIV GCCGGC 1 cut(s) 1727
NlaIV GGNNCC 4 cut(s) 579, 1437, 1610, 1732
NmeAIII GCCGAG 2 cut(s) 714, 1169
NmuCI GTSAC 4 cut(s) 260, 616, 676, 1639
NsiI ATGCAT 1 cut(s) 1885
NspI RCATGY 5 cut(s) 327, 684, 1750, 1831, 1887
NspV TTCGAA 1 cut(s) 1118
PaeI GCATGC 2 cut(s) 327, 1887
PaeR7I CTCGAG 1 cut(s) 447
PagI TCATGA 1 cut(s) 571
PciI ACATGT 2 cut(s) 1746, 1827
PciSI GCTCTTC 1 cut(s) 887
PctI GAATGC 2 cut(s) 1218, 1524
PdiI GCCGGC 1 cut(s) 1729
PdmI GAANNNNTTC 3 cut(s) 11, 1298, 1589
PfeI GAWTC 3 cut(s) 400, 733, 800
PflMI CCANNNNNTGG 1 cut(s) 1785
PkrI GCNGC 2 cut(s) 1359, 1688
PleI GAGTC 3 cut(s) 787, 806, 1324
PpsI GAGTC 3 cut(s) 787, 806, 1324
PscI ACATGT 2 cut(s) 1746, 1827
PshBI ATTAAT 1 cut(s) 633
Psp6I CCWGG 1 cut(s) 1778
PspEI GGTNACC 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 1099
PspGI CCWGG 1 cut(s) 1778
PspN4I GGNNCC 4 cut(s) 579, 1437, 1610, 1732
PspPI GGNCC 1 cut(s) 988
PstNI CAGNNNCTG 2 cut(s) 204, 587
PsuI RGATCY 3 cut(s) 577, 1435, 1905
RsaI GTAC 8 cut(s) 626, 644, 1069, 1202, 1342, 1487, 1745, 1826
RsaNI GTAC 8 cut(s) 625, 643, 1068, 1201, 1341, 1486, 1744, 1825
RseI CAYNNNNRTG 6 cut(s) 741, 908, 996, 1496, 1576, 1580
SapI GCTCTTC 1 cut(s) 887
SatI GCNGC 2 cut(s) 1358, 1687
Sau3AI GATC 7 cut(s) 372, 409, 461, 577, 835, 1435, 1905
Sau96I GGNCC 1 cut(s) 988
SchI GAGTC 3 cut(s) 788, 806, 1325
ScrFI CCNGG 3 cut(s) 88, 113, 1780
SduI GDGCHC 3 cut(s) 695, 882, 1515
SfaNI GCATC 4 cut(s) 276, 285, 331, 1847
SfcI CTRYAG 2 cut(s) 1326, 1529
Sfr274I CTCGAG 1 cut(s) 447
SfuI TTCGAA 1 cut(s) 1118
SinI GGWCC 1 cut(s) 988
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 6 cut(s) 741, 908, 996, 1496, 1576, 1580
SmlI CTYRAG 1 cut(s) 447
SmoI CTYRAG 1 cut(s) 447
SphI GCATGC 2 cut(s) 327, 1887
Sse9I AATT 9 cut(s) 219, 274, 346, 750, 840, 862, 1428, 1659, 1833
SsiI CCGC 1 cut(s) 265
SspI AATATT 1 cut(s) 1281
SspMI CTAG 2 cut(s) 827, 1637
StyD4I CCNGG 3 cut(s) 86, 111, 1778
StyI CCWWGG 6 cut(s) 56, 73, 241, 826, 991, 1123
TaaI ACNGT 6 cut(s) 230, 309, 629, 877, 1072, 1533
TaiI ACGT 1 cut(s) 508
TaqI TCGA 5 cut(s) 375, 432, 448, 460, 1118
TaqII GACCGA 1 cut(s) 268
TasI AATT 9 cut(s) 219, 274, 346, 750, 840, 862, 1428, 1659, 1833
TatI WGTACW 3 cut(s) 642, 1200, 1743
TfiI GAWTC 3 cut(s) 400, 733, 800
TscAI CASTG 5 cut(s) 790, 1077, 1229, 1386, 1479
TseFI GTSAC 4 cut(s) 260, 616, 676, 1639
TseI GCWGC 2 cut(s) 1357, 1686
Tsp45I GTSAC 4 cut(s) 260, 616, 676, 1639
TspRI CASTG 5 cut(s) 790, 1077, 1229, 1386, 1479
Van91I CCANNNNNTGG 1 cut(s) 1785
VpaK11BI GGWCC 1 cut(s) 988
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 1 cut(s) 274
XceI RCATGY 5 cut(s) 327, 684, 1750, 1831, 1887
XcmI CCANNNNNNNNNTGG 1 cut(s) 1479
XhoI CTCGAG 1 cut(s) 447
XmaJI CCTAGG 1 cut(s) 826
XmnI GAANNNNTTC 3 cut(s) 11, 1298, 1589
XspI CTAG 2 cut(s) 827, 1637
Zsp2I ATGCAT 1 cut(s) 1885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.