Rroxscaffold_2G00148150
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003988
Physical Location & Seq
Forward (+)
85807867 .. 85810700
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148150.1

Sequence Viewer

Length: 1086 bp
ATGAGAGAGGCAGAGTATTGCAAAGTTGCATGCCGTGTAAAACTGGATGCTAATTCTGCGAAAAGTTTTAAGGAGAAGATCGATGGTGAATATCAAGTTAACATGATTCTTGATGATCTCCCTGTAGCAGTTGCACACAAAATGAGAAACTCTCAAGATGAGGGTCCATCCATGATATATAATCTTGGGTTTGTGGTTGGGATGAAGGGACATTATGCTGGAGAAAAAGAAGAAAGACATTTCATTAACAACCACCTGAGTTTTAAAGTCATGTATCATGAGGATCCAGAGACTGATTATGCTCACATTGTCGGTTTTGAGGTCACGCCGTTCAGTATTAATCACGAGTACAAGGAGTGGAATGGTGAGAAGACTAAGCTTGTCACATGCCAGCCGAGCACCAATACTTACCTTCATAGCCCACAAGGGATTTATACTGATGAAGAAATTGTTTTCACATATGATGTATCTTTTGAGTCCAGTGATATCAAGTGGGAATCGCGTTGGGACGCATACCTCTACCTAGGTGATGATCCAATTCATTGGTTTGCTATGGTCAATTCATTGATAACTGTGCTCTTCCTTTCCGGAGTGGTAGCCATGGTTGTGATGAGAACTCTTTACAAGGATATTACCAACTACAATCAGTTGGAGACCCAAGTTGAAGAAACAGGATGGAAACTGGTCCATGGAGATGTCTTTAGGCCACCCATCAATGCTAATATATTGTGTGTTTTTGTTGCGAATGGTCTCCAAGTCTTTGGTACGGTGCTCTTCACAATAACTCTTGCATTGCTGGGTTTCCTTTCACCTTGGAACCAAGGTGGGGTGATTAAAGCAATGGTTCTTTTGTGGATGTTCATGGGCTTATTTGGGGGATACTACTCGGCGCGCTTGTACAAGATGTTTAAAGGCATTCAGAGGAAGAAGATTGCAATAAAGACAGCCTTTATGTTCCCTGCCACATTTTTCGGAATATTTTTCATGTTGAATGTCTTCATTTGGGGGAGGAGTCTTTTGCTGCAGTGCCGTTTGGTACAATGTTTTCTCTGCTGCTCTTGTGGTTTGGGATATCAGTGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

361

Amino Acids

41.48

Weight (kDa)

6.26

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 1 - 338 4.8e-103 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 502, 892
AccIII TCCGGA 1 cut(s) 587
AclWI GGATC 3 cut(s) 278, 291, 527
AfaI GTAC 4 cut(s) 350, 766, 899, 1038
AfiI CCNNNNNNNGG 1 cut(s) 826
AgsI TTSAA 2 cut(s) 665, 991
AluBI AGCT 1 cut(s) 379
AluI AGCT 1 cut(s) 379
Alw21I GWGCWC 3 cut(s) 401, 579, 774
Alw26I GTCTC 3 cut(s) 284, 647, 755
AlwI GGATC 3 cut(s) 278, 291, 527
AlwNI CAGNNNCTG 1 cut(s) 293
Aor13HI TCCGGA 1 cut(s) 587
AoxI GGCC 1 cut(s) 704
ApeKI GCWGC 2 cut(s) 1021, 1053
AseI ATTAAT 1 cut(s) 339
Asp700I GAANNNNTTC 1 cut(s) 995
AspA2I CCTAGG 1 cut(s) 523
AspLEI GCGC 2 cut(s) 892, 894
AspS9I GGNCC 2 cut(s) 164, 685
AsuHPI GGTGA 5 cut(s) 98, 377, 539, 801, 841
AvaII GGWCC 2 cut(s) 164, 685
AvrII CCTAGG 1 cut(s) 523
BamHI GGATCC 1 cut(s) 283
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 2 cut(s) 377, 988
Bbv12I GWGCWC 3 cut(s) 401, 579, 774
BbvI GCAGC 2 cut(s) 1008, 1040
BccI CCATC 4 cut(s) 77, 175, 669, 719
BceAI ACGGC 3 cut(s) 18, 313, 1014
BciVI GTATCC 1 cut(s) 872
BcoDI GTCTC 3 cut(s) 284, 647, 755
BfaI CTAG 1 cut(s) 524
BfmI CTRYAG 2 cut(s) 123, 1022
BfuI GTATCC 1 cut(s) 872
BisI GCNGC 2 cut(s) 1022, 1054
BlnI CCTAGG 1 cut(s) 523
BlsI GCNGC 2 cut(s) 1023, 1055
Bme18I GGWCC 2 cut(s) 164, 685
BmgT120I GGNCC 2 cut(s) 164, 685
BmiI GGNNCC 3 cut(s) 165, 285, 818
BmsI GCATC 1 cut(s) 37
BpiI GAAGAC 2 cut(s) 377, 988
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 81
BsaI GGTCTC 2 cut(s) 647, 755
BsaJI CCNNGG 5 cut(s) 523, 600, 688, 812, 820
BsaWI WCCGGW 1 cut(s) 587
Bsc4I CCNNNNNNNGG 1 cut(s) 826
Bse1I ACTGG 3 cut(s) 48, 480, 687
Bse3DI GCAATG 2 cut(s) 791, 846
BseAI TCCGGA 1 cut(s) 587
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 5 cut(s) 523, 600, 688, 812, 820
BseGI GGATG 5 cut(s) 52, 167, 207, 680, 861
BseLI CCNNNNNNNGG 1 cut(s) 826
BseMI GCAATG 2 cut(s) 791, 846
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 3 cut(s) 48, 480, 687
BsePI GCGCGC 1 cut(s) 890
BseRI GAGGAG 1 cut(s) 1024
BseXI GCAGC 2 cut(s) 1008, 1040
BseYI CCCAGC 1 cut(s) 796
Bsh1236I CGCG 2 cut(s) 502, 892
BshFI GGCC 1 cut(s) 706
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 3 cut(s) 401, 579, 774
BsiSI CCGG 1 cut(s) 588
BslFI GGGAC 2 cut(s) 222, 521
BslI CCNNNNNNNGG 1 cut(s) 826
BsmAI GTCTC 3 cut(s) 284, 647, 755
BsmFI GGGAC 2 cut(s) 222, 521
BsmI GAATGC 1 cut(s) 915
BsnI GGCC 1 cut(s) 706
Bso31I GGTCTC 2 cut(s) 647, 755
Bsp1286I GDGCHC 3 cut(s) 401, 579, 774
Bsp13I TCCGGA 1 cut(s) 587
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 4 cut(s) 78, 115, 283, 532
Bsp19I CCATGG 2 cut(s) 600, 688
BspANI GGCC 1 cut(s) 706
BspCNI CTCAG 1 cut(s) 249
BspDI ATCGAT 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 587
BspFNI CGCG 2 cut(s) 502, 892
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 165, 285, 818
BspMAI CTGCAG 1 cut(s) 1026
BspPI GGATC 3 cut(s) 278, 291, 527
BspQI GCTCTTC 2 cut(s) 584, 779
BspTNI GGTCTC 2 cut(s) 647, 755
BsrDI GCAATG 2 cut(s) 791, 846
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 3 cut(s) 48, 480, 687
BssECI CCNNGG 5 cut(s) 523, 600, 688, 812, 820
BssHII GCGCGC 1 cut(s) 890
BssMI GATC 4 cut(s) 78, 115, 283, 532
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 5 cut(s) 523, 600, 688, 812, 820
Bst2BI CACGAG 1 cut(s) 344
Bst4CI ACNGT 2 cut(s) 574, 769
Bst6I CTCTTC 2 cut(s) 584, 779
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 3 cut(s) 31, 392, 892
BstDEI CTNAG 2 cut(s) 257, 375
BstDSI CCRYGG 2 cut(s) 600, 688
BstF5I GGATG 5 cut(s) 52, 167, 207, 680, 861
BstFNI CGCG 2 cut(s) 502, 892
BstHHI GCGC 2 cut(s) 892, 894
BstKTI GATC 4 cut(s) 81, 118, 286, 535
BstMAI GTCTC 3 cut(s) 284, 647, 755
BstMBI GATC 4 cut(s) 78, 115, 283, 532
BstMWI GCNNNNNNNGC 2 cut(s) 56, 396
BstNSI RCATGY 2 cut(s) 33, 390
BstSFI CTRYAG 2 cut(s) 123, 1022
BstUI CGCG 2 cut(s) 502, 892
BstV1I GCAGC 2 cut(s) 1008, 1040
BstV2I GAAGAC 2 cut(s) 377, 988
BstX2I RGATCY 1 cut(s) 283
BstXI CCANNNNNNTGG 2 cut(s) 543, 761
BstYI RGATCY 1 cut(s) 283
Bsu15I ATCGAT 1 cut(s) 81
BsuI GTATCC 1 cut(s) 872
BsuRI GGCC 1 cut(s) 706
BsuTUI ATCGAT 1 cut(s) 81
BtgI CCRYGG 2 cut(s) 600, 688
BtsCI GGATG 5 cut(s) 52, 167, 207, 680, 861
BtsI GCAGTG 1 cut(s) 1031
BtsIMutI CAGTG 3 cut(s) 487, 1031, 1082
Cac8I GCNNGC 3 cut(s) 31, 392, 892
CaiI CAGNNNCTG 1 cut(s) 293
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 892, 894
Cfr13I GGNCC 2 cut(s) 164, 685
ClaI ATCGAT 1 cut(s) 81
CseI GACGC 1 cut(s) 518
Csp6I GTAC 4 cut(s) 349, 765, 898, 1037
CviJI RGCY 7 cut(s) 379, 394, 420, 599, 706, 867, 947
CviKI_1 RGCY 7 cut(s) 379, 394, 420, 599, 706, 867, 947
CviQI GTAC 4 cut(s) 349, 765, 898, 1037
DdeI CTNAG 2 cut(s) 257, 375
DpnI GATC 4 cut(s) 80, 117, 285, 534
DpnII GATC 4 cut(s) 78, 115, 283, 532
DraI TTTAAA 2 cut(s) 265, 910
Eam1104I CTCTTC 2 cut(s) 584, 779
EarI CTCTTC 2 cut(s) 584, 779
Eco130I CCWWGG 5 cut(s) 523, 600, 688, 812, 820
Eco31I GGTCTC 2 cut(s) 647, 755
Eco32I GATATC 2 cut(s) 487, 1073
Eco47I GGWCC 2 cut(s) 164, 685
EcoRV GATATC 2 cut(s) 487, 1073
EcoT14I CCWWGG 5 cut(s) 523, 600, 688, 812, 820
ErhI CCWWGG 5 cut(s) 523, 600, 688, 812, 820
FalI AAGNNNNNCTT 2 cut(s) 932, 964
FaqI GGGAC 2 cut(s) 222, 521
FauNDI CATATG 1 cut(s) 460
Fnu4HI GCNGC 2 cut(s) 1022, 1054
FokI GGATG 5 cut(s) 59, 154, 214, 687, 868
Fsp4HI GCNGC 2 cut(s) 1022, 1054
FspBI CTAG 1 cut(s) 524
GlaI GCGC 2 cut(s) 891, 893
GluI GCNGC 2 cut(s) 1022, 1054
GsaI CCCAGC 1 cut(s) 800
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 1 cut(s) 706
HapII CCGG 1 cut(s) 588
HgaI GACGC 1 cut(s) 518
HhaI GCGC 2 cut(s) 892, 894
Hin6I GCGC 2 cut(s) 890, 892
HinP1I GCGC 2 cut(s) 890, 892
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 4 cut(s) 106, 476, 497, 1012
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 1 cut(s) 588
HphI GGTGA 5 cut(s) 98, 377, 539, 801, 841
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 2 cut(s) 921, 974
Hpy188III TCNNGA 6 cut(s) 110, 155, 278, 287, 344, 588
Hpy8I GTNNAC 1 cut(s) 100
HpyAV CCTTC 2 cut(s) 199, 422
HpyCH4III ACNGT 2 cut(s) 574, 769
HpyCH4V TGCA 6 cut(s) 21, 29, 134, 791, 935, 1024
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 396
HpyF3I CTNAG 2 cut(s) 257, 375
HspAI GCGC 2 cut(s) 890, 892
Kpn2I TCCGGA 1 cut(s) 587
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 4 cut(s) 78, 115, 283, 532
LguI GCTCTTC 2 cut(s) 584, 779
Lsp1109I GCAGC 2 cut(s) 1008, 1040
LweI GCATC 1 cut(s) 37
MaeI CTAG 1 cut(s) 524
MaeIII GTNAC 2 cut(s) 322, 382
MalI GATC 4 cut(s) 80, 117, 285, 534
MboI GATC 4 cut(s) 78, 115, 283, 532
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 3 cut(s) 401, 579, 774
MluCI AATT 4 cut(s) 52, 447, 537, 559
MlyI GAGTC 2 cut(s) 485, 1021
MmeI TCCRAC 1 cut(s) 630
MnlI CCTC 6 cut(s) 154, 274, 313, 527, 915, 1002
MroI TCCGGA 1 cut(s) 587
MroXI GAANNNNTTC 1 cut(s) 995
MseI TTAA 7 cut(s) 69, 99, 246, 264, 339, 834, 909
MslI CAYNNNNRTG 2 cut(s) 605, 693
MspI CCGG 1 cut(s) 588
Mva1269I GAATGC 1 cut(s) 915
MvnI CGCG 2 cut(s) 502, 892
MwoI GCNNNNNNNGC 2 cut(s) 56, 396
NcoI CCATGG 2 cut(s) 600, 688
NdeI CATATG 1 cut(s) 460
NdeII GATC 4 cut(s) 78, 115, 283, 532
NlaIV GGNNCC 3 cut(s) 165, 285, 818
NmeAIII GCCGAG 2 cut(s) 420, 866
NmuCI GTSAC 2 cut(s) 322, 382
NspI RCATGY 2 cut(s) 33, 390
PaeI GCATGC 1 cut(s) 33
PagI TCATGA 1 cut(s) 277
PauI GCGCGC 1 cut(s) 890
PciSI GCTCTTC 2 cut(s) 584, 779
PctI GAATGC 1 cut(s) 915
PdmI GAANNNNTTC 1 cut(s) 995
PfeI GAWTC 2 cut(s) 106, 497
PkrI GCNGC 2 cut(s) 1023, 1055
PleI GAGTC 2 cut(s) 484, 1020
PpsI GAGTC 2 cut(s) 484, 1020
PshBI ATTAAT 1 cut(s) 339
PspFI CCCAGC 1 cut(s) 796
PspN4I GGNNCC 3 cut(s) 165, 285, 818
PspPI GGNCC 2 cut(s) 164, 685
PstI CTGCAG 1 cut(s) 1026
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 1 cut(s) 283
PteI GCGCGC 1 cut(s) 890
RsaI GTAC 4 cut(s) 350, 766, 899, 1038
RsaNI GTAC 4 cut(s) 349, 765, 898, 1037
RseI CAYNNNNRTG 2 cut(s) 605, 693
SapI GCTCTTC 2 cut(s) 584, 779
SaqAI TTAA 7 cut(s) 69, 99, 246, 264, 339, 834, 909
SatI GCNGC 2 cut(s) 1022, 1054
Sau3AI GATC 4 cut(s) 78, 115, 283, 532
Sau96I GGNCC 2 cut(s) 164, 685
SchI GAGTC 2 cut(s) 485, 1021
SduI GDGCHC 3 cut(s) 401, 579, 774
SetI ASST 9 cut(s) 258, 324, 381, 414, 519, 525, 529, 814, 826
SfaNI GCATC 1 cut(s) 37
SfcI CTRYAG 2 cut(s) 123, 1022
SinI GGWCC 2 cut(s) 164, 685
SmiMI CAYNNNNRTG 2 cut(s) 605, 693
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
SphI GCATGC 1 cut(s) 33
Sse9I AATT 4 cut(s) 52, 447, 537, 559
SspI AATATT 1 cut(s) 978
SspMI CTAG 1 cut(s) 524
StyI CCWWGG 5 cut(s) 523, 600, 688, 812, 820
TaaI ACNGT 2 cut(s) 574, 769
TaqI TCGA 1 cut(s) 81
TasI AATT 4 cut(s) 52, 447, 537, 559
TatI WGTACW 2 cut(s) 348, 897
TfiI GAWTC 2 cut(s) 106, 497
Tru1I TTAA 7 cut(s) 69, 99, 246, 264, 339, 834, 909
Tru9I TTAA 7 cut(s) 69, 99, 246, 264, 339, 834, 909
TscAI CASTG 3 cut(s) 487, 1031, 1082
TseFI GTSAC 2 cut(s) 322, 382
TseI GCWGC 2 cut(s) 1021, 1053
Tsp45I GTSAC 2 cut(s) 322, 382
TspDTI ATGAA 9 cut(s) 218, 232, 404, 456, 530, 552, 850, 973, 988
TspRI CASTG 3 cut(s) 487, 1031, 1082
VpaK11BI GGWCC 2 cut(s) 164, 685
VspI ATTAAT 1 cut(s) 339
XceI RCATGY 2 cut(s) 33, 390
XmaJI CCTAGG 1 cut(s) 523
XmnI GAANNNNTTC 1 cut(s) 995
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.