Rh2BG082300
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003988
Physical Location & Seq
Reverse (-)
6388752 .. 6389920
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG082300.1

Sequence Viewer

Length: 855 bp
ATGCTCATCACGATCATATTTGGTCTGCTTGGTTTCCTCTCACCTTCGAACAGAGGTGGGTTGATCACAGCAATGGTTCTTTCGTGGATGTTCATGGGCTTATTTGGGGGATACTACTCGGCGCACTTGTACAAGATGTTCAAAGGCACGCAATGGAAGAAGATTGCATTAAAGACAGCCTTTATGTTTCCTGTTACATTGATTGGAATTTTCTTCATGTTGAATGCCTTGATTTGGGAGGAGAAATCCTGTGGTTCAGTGCCACTGGGGACAATGTTTACCCTGGTACTCTTGTGGTCTGGAATATCGGCGCCATTGGTTTTCTTAGGCAGCTACTTTGGCTTCAAAATGTCACCAATTGATGATCCTATCAAAACTAATGAAATTCCAAGACAGATTCCTGGGCGACCATGGCACAACATAAAGCGGCTCTTGTCTATGCTATTTGGAAGCATTCTACCATTTAATGTAGTGCTCACTGAGTTCCTCTCTATCTGGACGTCTATATGGCCAGACCAATACTCTTTCGGGTTCCTCTTCATGGCCTTTGTTATACTTCTAGTCACTTGTTCCGAGATGGCTATTCTACTGTGCTACTTCCAACTTCGCAGCGAAGATTACAATTGGTGGTGGAGAGCATACCTGACTATTGGCTCTTCTGGTCTGTACATGTTTTCATACTCCATATTCTATTTCTTCACCAACATGGAGATTGAAAAGTTTGTTTCTGTCATCCTTTATTTCGGGTACATGTTAATGGGATCCTTTGCATTCTTTGTCCTAACGGGGACAATAGGCTTCGGTGCGTGCTTCTGGTTTGTTAGGAAGATCTATTCTTCTGTGAAGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

284

Amino Acids

32.78

Weight (kDa)

9.08

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 2 - 241 2.8e-84 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 310
AciI CCGC 1 cut(s) 427
AclWI GGATC 3 cut(s) 359, 756, 769
AcoI YGGCCR 1 cut(s) 509
AcsI RAATTY 2 cut(s) 207, 384
AcyI GRCGYC 2 cut(s) 311, 500
AfaI GTAC 4 cut(s) 131, 288, 668, 749
AfiI CCNNNNNNNGG 2 cut(s) 234, 541
AflIII ACRYGT 2 cut(s) 669, 750
AgsI TTSAA 4 cut(s) 142, 223, 346, 716
AjnI CCWGG 2 cut(s) 282, 400
AluBI AGCT 1 cut(s) 333
AluI AGCT 1 cut(s) 333
Alw21I GWGCWC 1 cut(s) 477
AlwI GGATC 3 cut(s) 359, 756, 769
AoxI GGCC 2 cut(s) 509, 543
ApeKI GCWGC 2 cut(s) 330, 609
ApoI RAATTY 2 cut(s) 207, 384
AspLEI GCGC 2 cut(s) 124, 313
AsuHPI GGTGA 3 cut(s) 33, 345, 691
AsuII TTCGAA 1 cut(s) 47
BaeI ACNNNNGTAYC 2 cut(s) 278, 311
BalI TGGCCA 1 cut(s) 511
BamHI GGATCC 1 cut(s) 761
BanI GGYRCC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 477
BbvI GCAGC 2 cut(s) 342, 621
BccI CCATC 1 cut(s) 571
BciT130I CCWGG 2 cut(s) 284, 402
BciVI GTATCC 1 cut(s) 104
BclI TGATCA 1 cut(s) 63
BfaI CTAG 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 314
BfuI GTATCC 1 cut(s) 104
BglII AGATCT 1 cut(s) 828
BisI GCNGC 3 cut(s) 331, 428, 610
BlsI GCNGC 3 cut(s) 332, 429, 611
Bme1390I CCNGG 2 cut(s) 284, 402
BmiI GGNNCC 3 cut(s) 312, 533, 763
BmrFI CCNGG 2 cut(s) 284, 402
BmrI ACTGGG 1 cut(s) 275
BmuI ACTGGG 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 473, 505
Bpu14I TTCGAA 1 cut(s) 47
BsaHI GRCGYC 2 cut(s) 311, 500
BsaJI CCNNGG 3 cut(s) 282, 401, 410
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 541
Bse1I ACTGG 1 cut(s) 270
Bse3DI GCAATG 2 cut(s) 78, 158
BseBI CCWGG 2 cut(s) 284, 402
BseDI CCNNGG 3 cut(s) 282, 401, 410
BseGI GGATG 2 cut(s) 93, 732
BseLI CCNNNNNNNGG 2 cut(s) 234, 541
BseMI GCAATG 2 cut(s) 78, 158
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 2 cut(s) 342, 621
BshFI GGCC 2 cut(s) 511, 545
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 477
BslFI GGGAC 2 cut(s) 283, 802
BslI CCNNNNNNNGG 2 cut(s) 234, 541
BsmFI GGGAC 2 cut(s) 283, 802
BsmI GAATGC 3 cut(s) 229, 453, 770
BsnI GGCC 2 cut(s) 511, 545
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 477
Bsp1407I TGTACA 2 cut(s) 129, 666
Bsp143I GATC 5 cut(s) 12, 63, 364, 761, 828
Bsp19I CCATGG 1 cut(s) 410
BspACI CCGC 1 cut(s) 427
BspANI GGCC 2 cut(s) 511, 545
BspCNI CTCAG 1 cut(s) 472
BspLI GGNNCC 3 cut(s) 312, 533, 763
BspPI GGATC 3 cut(s) 359, 756, 769
BspQI GCTCTTC 1 cut(s) 661
BspT104I TTCGAA 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 78, 158
BsrGI TGTACA 2 cut(s) 129, 666
BsrI ACTGG 1 cut(s) 270
BssECI CCNNGG 3 cut(s) 282, 401, 410
BssMI GATC 5 cut(s) 12, 63, 364, 761, 828
BssNI GRCGYC 2 cut(s) 311, 500
BssT1I CCWWGG 1 cut(s) 410
Bst2UI CCWGG 2 cut(s) 284, 402
Bst4CI ACNGT 1 cut(s) 591
Bst6I CTCTTC 2 cut(s) 542, 661
BstACI GRCGYC 2 cut(s) 311, 500
BstAUI TGTACA 2 cut(s) 129, 666
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 2 cut(s) 149, 808
BstDEI CTNAG 2 cut(s) 325, 480
BstDSI CCRYGG 1 cut(s) 410
BstF5I GGATG 2 cut(s) 93, 732
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 2 cut(s) 124, 313
BstKTI GATC 5 cut(s) 15, 66, 367, 764, 831
BstMBI GATC 5 cut(s) 12, 63, 364, 761, 828
BstMWI GCNNNNNNNGC 2 cut(s) 339, 412
BstNI CCWGG 2 cut(s) 284, 402
BstNSI RCATGY 2 cut(s) 673, 754
BstSCI CCNGG 2 cut(s) 282, 400
BstV1I GCAGC 2 cut(s) 342, 621
BstX2I RGATCY 2 cut(s) 761, 828
BstYI RGATCY 2 cut(s) 761, 828
BsuI GTATCC 1 cut(s) 104
BsuRI GGCC 2 cut(s) 511, 545
BtgI CCRYGG 1 cut(s) 410
BtsCI GGATG 2 cut(s) 93, 732
BtsIMutI CAGTG 3 cut(s) 263, 264, 477
Cac8I GCNNGC 2 cut(s) 149, 808
CfoI GCGC 2 cut(s) 124, 313
Csp6I GTAC 4 cut(s) 130, 287, 667, 748
CviAII CATG 7 cut(s) 94, 217, 411, 541, 670, 706, 751
CviQI GTAC 4 cut(s) 130, 287, 667, 748
DdeI CTNAG 2 cut(s) 325, 480
DinI GGCGCC 1 cut(s) 312
DpnI GATC 5 cut(s) 14, 65, 366, 763, 830
DpnII GATC 5 cut(s) 12, 63, 364, 761, 828
EaeI YGGCCR 1 cut(s) 509
Eam1104I CTCTTC 2 cut(s) 542, 661
EarI CTCTTC 2 cut(s) 542, 661
Eco130I CCWWGG 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 282, 400
EcoT14I CCWWGG 1 cut(s) 410
EgeI GGCGCC 1 cut(s) 312
EheI GGCGCC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 410
FaeI CATG 7 cut(s) 97, 220, 414, 544, 673, 709, 754
FalI AAGNNNNNCTT 4 cut(s) 164, 196, 416, 448
FaqI GGGAC 2 cut(s) 283, 802
FatI CATG 7 cut(s) 93, 216, 410, 540, 669, 705, 750
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 331, 428, 610
FokI GGATG 2 cut(s) 100, 719
Fsp4HI GCNGC 3 cut(s) 331, 428, 610
FspBI CTAG 1 cut(s) 560
GlaI GCGC 2 cut(s) 123, 312
GluI GCNGC 3 cut(s) 331, 428, 610
HaeII RGCGCY 1 cut(s) 314
HaeIII GGCC 2 cut(s) 511, 545
HhaI GCGC 2 cut(s) 124, 313
Hin1I GRCGYC 2 cut(s) 311, 500
Hin1II CATG 7 cut(s) 97, 220, 414, 544, 673, 709, 754
Hin6I GCGC 2 cut(s) 122, 311
HinP1I GCGC 2 cut(s) 122, 311
HinfI GANTC 1 cut(s) 397
HphI GGTGA 3 cut(s) 33, 345, 691
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 1 cut(s) 574
Hpy188III TCNNGA 3 cut(s) 10, 300, 496
Hpy8I GTNNAC 1 cut(s) 279
HpyAV CCTTC 1 cut(s) 54
HpyCH4III ACNGT 1 cut(s) 591
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 2 cut(s) 167, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 339, 412
HpyF3I CTNAG 2 cut(s) 325, 480
HpySE526I ACGT 1 cut(s) 500
Hsp92I GRCGYC 2 cut(s) 311, 500
Hsp92II CATG 7 cut(s) 97, 220, 414, 544, 673, 709, 754
HspAI GCGC 2 cut(s) 122, 311
KasI GGCGCC 1 cut(s) 310
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 5 cut(s) 12, 63, 364, 761, 828
LguI GCTCTTC 1 cut(s) 661
Lsp1109I GCAGC 2 cut(s) 342, 621
MaeI CTAG 1 cut(s) 560
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 3 cut(s) 193, 351, 562
MalI GATC 5 cut(s) 14, 65, 366, 763, 830
MboI GATC 5 cut(s) 12, 63, 364, 761, 828
MboII GAAGA 9 cut(s) 169, 172, 205, 529, 626, 648, 688, 828, 838
MfeI CAATTG 2 cut(s) 357, 622
MflI RGATCY 2 cut(s) 761, 828
MhlI GDGCHC 1 cut(s) 477
MlsI TGGCCA 1 cut(s) 511
MluCI AATT 4 cut(s) 207, 357, 384, 622
MluNI TGGCCA 1 cut(s) 511
Mly113I GGCGCC 1 cut(s) 311
MmeI TCCRAC 1 cut(s) 625
MnlI CCTC 5 cut(s) 47, 47, 232, 497, 545
Mox20I TGGCCA 1 cut(s) 511
MscI TGGCCA 1 cut(s) 511
MseI TTAA 3 cut(s) 170, 465, 755
MslI CAYNNNNRTG 3 cut(s) 71, 704, 755
Msp20I TGGCCA 1 cut(s) 511
MspR9I CCNGG 2 cut(s) 284, 402
MunI CAATTG 2 cut(s) 357, 622
Mva1269I GAATGC 3 cut(s) 229, 453, 770
MvaI CCWGG 2 cut(s) 284, 402
MwoI GCNNNNNNNGC 2 cut(s) 339, 412
NarI GGCGCC 1 cut(s) 311
NcoI CCATGG 1 cut(s) 410
NdeII GATC 5 cut(s) 12, 63, 364, 761, 828
NlaIII CATG 7 cut(s) 97, 220, 414, 544, 673, 709, 754
NlaIV GGNNCC 3 cut(s) 312, 533, 763
NmeAIII GCCGAG 1 cut(s) 98
NmuCI GTSAC 2 cut(s) 351, 562
NspI RCATGY 2 cut(s) 673, 754
NspV TTCGAA 1 cut(s) 47
PciI ACATGT 2 cut(s) 669, 750
PciSI GCTCTTC 1 cut(s) 661
PctI GAATGC 3 cut(s) 229, 453, 770
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 3 cut(s) 332, 429, 611
PluTI GGCGCC 1 cut(s) 314
PscI ACATGT 2 cut(s) 669, 750
Psp6I CCWGG 2 cut(s) 282, 400
PspGI CCWGG 2 cut(s) 282, 400
PspN4I GGNNCC 3 cut(s) 312, 533, 763
PsrI GAACNNNNNNTAC 2 cut(s) 122, 154
PsuI RGATCY 2 cut(s) 761, 828
RsaI GTAC 4 cut(s) 131, 288, 668, 749
RsaNI GTAC 4 cut(s) 130, 287, 667, 748
RseI CAYNNNNRTG 3 cut(s) 71, 704, 755
SapI GCTCTTC 1 cut(s) 661
SaqAI TTAA 3 cut(s) 170, 465, 755
SatI GCNGC 3 cut(s) 331, 428, 610
Sau3AI GATC 5 cut(s) 12, 63, 364, 761, 828
ScrFI CCNGG 2 cut(s) 284, 402
SduI GDGCHC 1 cut(s) 477
SetI ASST 5 cut(s) 46, 58, 335, 503, 645
SfoI GGCGCC 1 cut(s) 312
SfuI TTCGAA 1 cut(s) 47
SmiMI CAYNNNNRTG 3 cut(s) 71, 704, 755
Sse9I AATT 4 cut(s) 207, 357, 384, 622
SsiI CCGC 1 cut(s) 427
SspDI GGCGCC 1 cut(s) 310
SspMI CTAG 1 cut(s) 560
StyD4I CCNGG 2 cut(s) 282, 400
StyI CCWWGG 1 cut(s) 410
TaaI ACNGT 1 cut(s) 591
TaiI ACGT 1 cut(s) 503
TaqI TCGA 1 cut(s) 47
TasI AATT 4 cut(s) 207, 357, 384, 622
TatI WGTACW 2 cut(s) 129, 666
TauI GCSGC 1 cut(s) 430
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 3 cut(s) 170, 465, 755
Tru9I TTAA 3 cut(s) 170, 465, 755
TscAI CASTG 3 cut(s) 264, 270, 484
TseFI GTSAC 2 cut(s) 351, 562
TseI GCWGC 2 cut(s) 330, 609
Tsp45I GTSAC 2 cut(s) 351, 562
TspDTI ATGAA 5 cut(s) 82, 205, 396, 529, 666
TspRI CASTG 3 cut(s) 264, 270, 484
XapI RAATTY 2 cut(s) 207, 384
XceI RCATGY 2 cut(s) 673, 754
XspI CTAG 1 cut(s) 560
ZraI GACGTC 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.