Rw2G006550
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003988
Physical Location & Seq
Forward (+)
6316507 .. 6320191
3685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006550.1

Sequence Viewer

Length: 1866 bp
ATGGCTTATCATGTTTTATTCCTACTAATGATCGCGCTGCATACAAGAGGAACTCGAGGTCGAGAAGATACACTGGAAGTAAAGGTTAATAAGCTATCATCCATCAAGACACTGCTTCCCTATGACTACTATTCCCTTGGTTACTGCAAGCCTCCAGTAATCACCAATAGTGCTTTAAGCCTTGGAGAAGTTCTTCGAGGTGACCGCATCAAGAATTCAATCTATACTCTTAAGATGAGAGAGGCAGAATACTGCAAAGTCGCATGCCGTGTGAAACTGGATGCTAATTCTGCCAAAAAGTTTAGGGAGAAAATCGATGATCAATATGTAGTTAATATGATTCTTGATAATCTTCCTGCTGCAATTACATTCGAAGCGAGTGACTGGAATGGGAATAAATCAAAGATACCTGTAACTGCACTCCCAGTTGGGCGGAAGGGATATGCCACTGAAGGCAGTGAGGAAAGACACTTCATTTACAATCACCTGAGATTCAACGTCATGTACCATGAGGATCCGGAGACCAAATTTTCTCGCATTGTTGGTTTTGAGATTACACCATACAGTGTTAATCATGAGTACAAGAAGTGGGAAAATGAGAAAACTGAGTTGGTCACCTGCACGACCAATTCTGACCTTTTAATTAGCAATTTACCGCAAGAGATTGATACTGATAAAGAAGTTGTCTTTACATATGACGTATTGTTCGAGTCCAGTGTTGTCAAGTGGGAATCACGGTGGGACTCATACCTCTCCAGGGGTACTGATAATCAAATCCATTGGTTATCTATGTTCAACTCATTGTGGACTCTGCTCCTTTCTGGAGTAATAGCCATGGTTGTGGTGCGAACTCTTTACAAGGATATCAACAGCTACAATCAGTTGGAGACCCAAGATGAGGCTCAGGAACTAACAGGATGGAAACTAGTCCATGCAGATGTTTTCAGGCCACCCCCCAACGCTAATTTATTGTGTGTTTGTGTTGGGAATGGTGTCCAATTCTTTGGGACGATGCTCATCACGATCATATTTGGTCTGCTTGGTTTCCTTTCACCTTCGAACAGAGGTGGGTTGATCACAGCAATGGTTCTTTCGTGGATGCTCATGGGCTTATTTGGGGGATACTACTCGGCGCACTTGTACAAGATGTTCAAAGGCACGCAATGGAAGAAGATTGCATTAAAGACAGCCATTGTGTTTCCTGTTACATTGATTGGAATTTTCTTCATGTTGAATGCCTTGATTTGGGAAGAGAAATCCTGTGGTTCAGTGCCACTGGGGACAATGTTTACCCTGGTACTCTTGTGGTCTGGAATATCAGCGCCATTGGTTTTCTTAGGCAGCTACTTTGGCTTCAAAATGTCACCAATTGATGATCCTATCAAAACTAATGAAATTCCAAGACAGATTCCTGAGCGACCATGGCACAACATAAAGCGGCTCTTGTCTATGCTATTTGGAAGCATTCTACCGTTTAATGTAGTGCTCACTGAGTTCCTCTCTATCTGGACGTCTATATGGCCAGACCAATACTCTTTCGGGTTCCTCTTCATGGCCTTTGTTATACTTCTAGTCACTTGTTCCGAGATGGCTATTCTACTGTGCTACTTCCAACTTCGCAGCGAAGATTACAATTGGTGGTGGAGAGCATACCTGACTATTGGCTCTTCTGGTCTGTACATGTTTTCATACTCCATATTCTATTTCTTCACCAACATGGAGATTGAAAAGTTTGTTTCTGTCATCCTTTATTTCGGGTACATGTTAATGGGATCCTTTGCATTCTTTGTCCTAACGGGGACAATAGGCTTCGGTGCGTGCTTCTGGTTTGTTAGGAAGATCTATTCTTCTGTGAAGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

621

Amino Acids

71.46

Weight (kDa)

7.53

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 40 - 578 2e-181 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 626
AatII GACGTC 1 cut(s) 1514
Acc36I ACCTGC 1 cut(s) 626
AccII CGCG 1 cut(s) 35
AccIII TCCGGA 1 cut(s) 517
AciI CCGC 4 cut(s) 205, 433, 656, 1438
AclWI GGATC 5 cut(s) 509, 522, 1370, 1767, 1780
AcoI YGGCCR 1 cut(s) 1520
AcsI RAATTY 4 cut(s) 214, 527, 1218, 1395
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 1511
AfaI GTAC 7 cut(s) 506, 581, 763, 1142, 1299, 1679, 1760
AfiI CCNNNNNNNGG 4 cut(s) 757, 898, 1245, 1552
AflII CTTAAG 1 cut(s) 230
AflIII ACRYGT 2 cut(s) 1680, 1761
AgsI TTSAA 7 cut(s) 219, 496, 796, 1153, 1234, 1357, 1727
AhlI ACTAGT 1 cut(s) 925
AjnI CCWGG 2 cut(s) 755, 1293
AjuI GAANNNNNNNTTGG 2 cut(s) 593, 625
AluBI AGCT 3 cut(s) 94, 873, 1344
AluI AGCT 3 cut(s) 94, 873, 1344
Alw21I GWGCWC 1 cut(s) 1488
Alw26I GTCTC 2 cut(s) 515, 881
AlwI GGATC 5 cut(s) 509, 522, 1370, 1767, 1780
Ama87I CYCGRG 1 cut(s) 54
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 3 cut(s) 947, 1520, 1554
ApeKI GCWGC 4 cut(s) 37, 359, 1341, 1620
ApoI RAATTY 4 cut(s) 214, 527, 1218, 1395
ArsI GACNNNNNNTTYG 2 cut(s) 689, 721
Asp700I GAANNNNTTC 1 cut(s) 192
AspLEI GCGC 3 cut(s) 37, 1135, 1324
AsuHPI GGTGA 7 cut(s) 154, 212, 476, 607, 1044, 1356, 1702
AsuII TTCGAA 2 cut(s) 372, 1058
AvaI CYCGRG 1 cut(s) 54
BaeI ACNNNNGTAYC 2 cut(s) 1289, 1322
BalI TGGCCA 1 cut(s) 1522
BamHI GGATCC 2 cut(s) 514, 1772
Bbv12I GWGCWC 1 cut(s) 1488
BbvI GCAGC 4 cut(s) 24, 346, 1353, 1632
BccI CCATC 3 cut(s) 110, 912, 1582
BceAI ACGGC 1 cut(s) 252
BciT130I CCWGG 2 cut(s) 757, 1295
BciVI GTATCC 1 cut(s) 1115
BclI TGATCA 2 cut(s) 319, 1074
BcoDI GTCTC 2 cut(s) 515, 881
BcuI ACTAGT 1 cut(s) 925
BfaI CTAG 2 cut(s) 926, 1571
BfoI RGCGCY 1 cut(s) 1325
BfrI CTTAAG 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 626
BfuI GTATCC 1 cut(s) 1115
BglII AGATCT 1 cut(s) 1839
BisI GCNGC 5 cut(s) 38, 360, 1342, 1439, 1621
BlsI GCNGC 5 cut(s) 39, 361, 1343, 1440, 1622
Bme1390I CCNGG 2 cut(s) 757, 1295
BmeT110I CYCGRG 1 cut(s) 54
BmiI GGNNCC 3 cut(s) 516, 1544, 1774
BmrFI CCNGG 2 cut(s) 757, 1295
BmrI ACTGGG 2 cut(s) 419, 1286
BmsI GCATC 4 cut(s) 216, 271, 1002, 1089
BmuI ACTGGG 2 cut(s) 419, 1286
BplI GAGNNNNNCTC 2 cut(s) 1484, 1516
BpmI CTGGAG 3 cut(s) 138, 739, 843
Bpu10I CCTNAGC 2 cut(s) 903, 1413
Bpu14I TTCGAA 2 cut(s) 372, 1058
Bsa29I ATCGAT 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 1016
BsaHI GRCGYC 1 cut(s) 1511
BsaI GGTCTC 2 cut(s) 515, 881
BsaJI CCNNGG 6 cut(s) 136, 181, 756, 834, 1293, 1421
BsaWI WCCGGW 1 cut(s) 517
Bsc4I CCNNNNNNNGG 4 cut(s) 757, 898, 1245, 1552
Bse1I ACTGG 7 cut(s) 78, 155, 282, 389, 425, 714, 1281
Bse3DI GCAATG 2 cut(s) 1089, 1169
Bse8I GATNNNNATC 1 cut(s) 1016
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 2 cut(s) 757, 1295
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 6 cut(s) 136, 181, 756, 834, 1293, 1421
BseGI GGATG 5 cut(s) 98, 286, 923, 1104, 1743
BseJI GATNNNNATC 1 cut(s) 1016
BseLI CCNNNNNNNGG 4 cut(s) 757, 898, 1245, 1552
BseMI GCAATG 2 cut(s) 1089, 1169
BseMII CTCAG 5 cut(s) 479, 597, 917, 1404, 1482
BseNI ACTGG 7 cut(s) 78, 155, 282, 389, 425, 714, 1281
BseXI GCAGC 4 cut(s) 24, 346, 1353, 1632
BsgI GTGCAG 2 cut(s) 402, 604
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 3 cut(s) 949, 1522, 1556
BshVI ATCGAT 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 1488
BsiHKCI CYCGRG 1 cut(s) 54
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 4 cut(s) 755, 1021, 1294, 1813
BslI CCNNNNNNNGG 4 cut(s) 757, 898, 1245, 1552
BsmAI GTCTC 2 cut(s) 515, 881
BsmFI GGGAC 4 cut(s) 755, 1021, 1294, 1813
BsmI GAATGC 3 cut(s) 1240, 1464, 1781
BsnI GGCC 3 cut(s) 949, 1522, 1556
Bso31I GGTCTC 2 cut(s) 515, 881
BsoBI CYCGRG 1 cut(s) 54
Bsp119I TTCGAA 2 cut(s) 372, 1058
Bsp1286I GDGCHC 1 cut(s) 1488
Bsp13I TCCGGA 1 cut(s) 517
Bsp1407I TGTACA 2 cut(s) 1140, 1677
Bsp143I GATC 8 cut(s) 30, 319, 514, 1023, 1074, 1375, 1772, 1839
Bsp19I CCATGG 2 cut(s) 834, 1421
BspACI CCGC 4 cut(s) 205, 433, 656, 1438
BspANI GGCC 3 cut(s) 949, 1522, 1556
BspCNI CTCAG 5 cut(s) 480, 598, 916, 1405, 1483
BspDI ATCGAT 1 cut(s) 315
BspEI TCCGGA 1 cut(s) 517
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 516, 1544, 1774
BspMI ACCTGC 1 cut(s) 626
BspPI GGATC 5 cut(s) 509, 522, 1370, 1767, 1780
BspQI GCTCTTC 1 cut(s) 1672
BspT104I TTCGAA 2 cut(s) 372, 1058
BspTI CTTAAG 1 cut(s) 230
BspTNI GGTCTC 2 cut(s) 515, 881
BsrDI GCAATG 2 cut(s) 1089, 1169
BsrGI TGTACA 2 cut(s) 1140, 1677
BsrI ACTGG 7 cut(s) 78, 155, 282, 389, 425, 714, 1281
BssECI CCNNGG 6 cut(s) 136, 181, 756, 834, 1293, 1421
BssMI GATC 8 cut(s) 30, 319, 514, 1023, 1074, 1375, 1772, 1839
BssNI GRCGYC 1 cut(s) 1511
BssT1I CCWWGG 4 cut(s) 136, 181, 834, 1421
Bst2UI CCWGG 2 cut(s) 757, 1295
Bst4CI ACNGT 4 cut(s) 566, 738, 1473, 1602
Bst6I CTCTTC 3 cut(s) 1245, 1553, 1672
BstACI GRCGYC 1 cut(s) 1511
BstAFI CTTAAG 1 cut(s) 230
BstAUI TGTACA 2 cut(s) 1140, 1677
BstBI TTCGAA 2 cut(s) 372, 1058
BstC8I GCNNGC 4 cut(s) 149, 265, 1160, 1819
BstDEI CTNAG 6 cut(s) 488, 606, 903, 1336, 1413, 1491
BstDSI CCRYGG 2 cut(s) 834, 1421
BstEII GGTNACC 2 cut(s) 200, 613
BstF5I GGATG 5 cut(s) 98, 286, 923, 1104, 1743
BstFNI CGCG 1 cut(s) 35
BstH2I RGCGCY 1 cut(s) 1325
BstHHI GCGC 3 cut(s) 37, 1135, 1324
BstKTI GATC 8 cut(s) 33, 322, 517, 1026, 1077, 1378, 1775, 1842
BstMAI GTCTC 2 cut(s) 515, 881
BstMBI GATC 8 cut(s) 30, 319, 514, 1023, 1074, 1375, 1772, 1839
BstMWI GCNNNNNNNGC 3 cut(s) 290, 1350, 1423
BstNI CCWGG 2 cut(s) 757, 1295
BstNSI RCATGY 3 cut(s) 267, 1684, 1765
BstPI GGTNACC 2 cut(s) 200, 613
BstSCI CCNGG 2 cut(s) 755, 1293
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 4 cut(s) 24, 346, 1353, 1632
BstX2I RGATCY 3 cut(s) 514, 1772, 1839
BstXI CCANNNNNNTGG 2 cut(s) 841, 1004
BstYI RGATCY 3 cut(s) 514, 1772, 1839
Bsu15I ATCGAT 1 cut(s) 315
BsuI GTATCC 1 cut(s) 1115
BsuRI GGCC 3 cut(s) 949, 1522, 1556
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 2 cut(s) 834, 1421
BtsCI GGATG 5 cut(s) 98, 286, 923, 1104, 1743
BtsI GCAGTG 2 cut(s) 110, 463
BtsIMutI CAGTG 9 cut(s) 71, 110, 447, 463, 571, 721, 1274, 1275, 1488
BveI ACCTGC 1 cut(s) 626
Cac8I GCNNGC 4 cut(s) 149, 265, 1160, 1819
CciI TCATGA 1 cut(s) 574
CfoI GCGC 3 cut(s) 37, 1135, 1324
ClaI ATCGAT 1 cut(s) 315
Csp6I GTAC 7 cut(s) 505, 580, 762, 1141, 1298, 1678, 1759
CviQI GTAC 7 cut(s) 505, 580, 762, 1141, 1298, 1678, 1759
DdeI CTNAG 6 cut(s) 488, 606, 903, 1336, 1413, 1491
DpnI GATC 8 cut(s) 32, 321, 516, 1025, 1076, 1377, 1774, 1841
DpnII GATC 8 cut(s) 30, 319, 514, 1023, 1074, 1375, 1772, 1839
EaeI YGGCCR 1 cut(s) 1520
Eam1104I CTCTTC 3 cut(s) 1245, 1553, 1672
EarI CTCTTC 3 cut(s) 1245, 1553, 1672
EciI GGCGGA 1 cut(s) 448
Eco130I CCWWGG 4 cut(s) 136, 181, 834, 1421
Eco31I GGTCTC 2 cut(s) 515, 881
Eco32I GATATC 1 cut(s) 865
Eco57I CTGAAG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 54
Eco91I GGTNACC 2 cut(s) 200, 613
EcoO65I GGTNACC 2 cut(s) 200, 613
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 755, 1293
EcoRV GATATC 1 cut(s) 865
EcoT14I CCWWGG 4 cut(s) 136, 181, 834, 1421
ErhI CCWWGG 4 cut(s) 136, 181, 834, 1421
FalI AAGNNNNNCTT 2 cut(s) 1427, 1459
FaqI GGGAC 4 cut(s) 755, 1021, 1294, 1813
FauNDI CATATG 1 cut(s) 694
FbaI TGATCA 2 cut(s) 319, 1074
Fnu4HI GCNGC 5 cut(s) 38, 360, 1342, 1439, 1621
FokI GGATG 5 cut(s) 85, 293, 930, 1111, 1730
Fsp4HI GCNGC 5 cut(s) 38, 360, 1342, 1439, 1621
FspBI CTAG 2 cut(s) 926, 1571
GlaI GCGC 3 cut(s) 36, 1134, 1323
GluI GCNGC 5 cut(s) 38, 360, 1342, 1439, 1621
GsuI CTGGAG 3 cut(s) 138, 739, 843
HaeII RGCGCY 1 cut(s) 1325
HaeIII GGCC 3 cut(s) 949, 1522, 1556
HapII CCGG 1 cut(s) 518
HhaI GCGC 3 cut(s) 37, 1135, 1324
Hin1I GRCGYC 1 cut(s) 1511
Hin6I GCGC 3 cut(s) 35, 1133, 1322
HinP1I GCGC 3 cut(s) 35, 1133, 1322
HinfI GANTC 7 cut(s) 340, 492, 710, 731, 743, 808, 1408
HpaII CCGG 1 cut(s) 518
HphI GGTGA 7 cut(s) 154, 212, 476, 607, 1044, 1356, 1702
Hpy166II GTNNAC 2 cut(s) 807, 1290
Hpy188I TCNGA 2 cut(s) 634, 1585
Hpy8I GTNNAC 2 cut(s) 807, 1290
HpyAV CCTTC 3 cut(s) 430, 446, 1065
HpyCH4III ACNGT 4 cut(s) 566, 738, 1473, 1602
HpyCH4IV ACGT 3 cut(s) 498, 699, 1511
HpyCH4V TGCA 9 cut(s) 40, 147, 255, 362, 419, 621, 935, 1178, 1781
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 1350, 1423
HpyF3I CTNAG 6 cut(s) 488, 606, 903, 1336, 1413, 1491
HpySE526I ACGT 3 cut(s) 498, 699, 1511
Hsp92I GRCGYC 1 cut(s) 1511
HspAI GCGC 3 cut(s) 35, 1133, 1322
Kpn2I TCCGGA 1 cut(s) 517
Ksp22I TGATCA 2 cut(s) 319, 1074
Kzo9I GATC 8 cut(s) 30, 319, 514, 1023, 1074, 1375, 1772, 1839
LguI GCTCTTC 1 cut(s) 1672
LmnI GCTCC 1 cut(s) 819
Lsp1109I GCAGC 4 cut(s) 24, 346, 1353, 1632
LweI GCATC 4 cut(s) 216, 271, 1002, 1089
MaeI CTAG 2 cut(s) 926, 1571
MaeII ACGT 3 cut(s) 498, 699, 1511
MaeIII GTNAC 8 cut(s) 140, 200, 380, 412, 613, 1204, 1362, 1573
MalI GATC 8 cut(s) 32, 321, 516, 1025, 1076, 1377, 1774, 1841
MboI GATC 8 cut(s) 30, 319, 514, 1023, 1074, 1375, 1772, 1839
MfeI CAATTG 2 cut(s) 1368, 1633
MflI RGATCY 3 cut(s) 514, 1772, 1839
MhlI GDGCHC 1 cut(s) 1488
MlsI TGGCCA 1 cut(s) 1522
MluNI TGGCCA 1 cut(s) 1522
MlyI GAGTC 3 cut(s) 719, 737, 802
MmeI TCCRAC 2 cut(s) 864, 1636
Mox20I TGGCCA 1 cut(s) 1522
MroI TCCGGA 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 192
MscI TGGCCA 1 cut(s) 1522
MseI TTAA 9 cut(s) 87, 176, 231, 333, 570, 641, 1181, 1476, 1766
MslI CAYNNNNRTG 5 cut(s) 839, 936, 1082, 1715, 1766
Msp20I TGGCCA 1 cut(s) 1522
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 2 cut(s) 757, 1295
MunI CAATTG 2 cut(s) 1368, 1633
Mva1269I GAATGC 3 cut(s) 1240, 1464, 1781
MvaI CCWGG 2 cut(s) 757, 1295
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 3 cut(s) 290, 1350, 1423
NcoI CCATGG 2 cut(s) 834, 1421
NdeI CATATG 1 cut(s) 694
NdeII GATC 8 cut(s) 30, 319, 514, 1023, 1074, 1375, 1772, 1839
NlaIV GGNNCC 3 cut(s) 516, 1544, 1774
NmeAIII GCCGAG 1 cut(s) 1109
NmuCI GTSAC 5 cut(s) 200, 380, 613, 1362, 1573
NspI RCATGY 3 cut(s) 267, 1684, 1765
NspV TTCGAA 2 cut(s) 372, 1058
PaeI GCATGC 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 54
PagI TCATGA 1 cut(s) 574
PaqCI CACCTGC 1 cut(s) 626
PciI ACATGT 2 cut(s) 1680, 1761
PciSI GCTCTTC 1 cut(s) 1672
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PctI GAATGC 3 cut(s) 1240, 1464, 1781
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 4 cut(s) 340, 492, 731, 1408
PkrI GCNGC 5 cut(s) 39, 361, 1343, 1440, 1622
PleI GAGTC 3 cut(s) 718, 737, 802
PpsI GAGTC 3 cut(s) 718, 737, 802
PscI ACATGT 2 cut(s) 1680, 1761
Psp6I CCWGG 2 cut(s) 755, 1293
PspEI GGTNACC 2 cut(s) 200, 613
PspGI CCWGG 2 cut(s) 755, 1293
PspN4I GGNNCC 3 cut(s) 516, 1544, 1774
PspXI VCTCGAGB 1 cut(s) 54
PsrI GAACNNNNNNTAC 2 cut(s) 1133, 1165
PsuI RGATCY 3 cut(s) 514, 1772, 1839
RsaI GTAC 7 cut(s) 506, 581, 763, 1142, 1299, 1679, 1760
RsaNI GTAC 7 cut(s) 505, 580, 762, 1141, 1298, 1678, 1759
RseI CAYNNNNRTG 5 cut(s) 839, 936, 1082, 1715, 1766
SapI GCTCTTC 1 cut(s) 1672
SaqAI TTAA 9 cut(s) 87, 176, 231, 333, 570, 641, 1181, 1476, 1766
SatI GCNGC 5 cut(s) 38, 360, 1342, 1439, 1621
Sau3AI GATC 8 cut(s) 30, 319, 514, 1023, 1074, 1375, 1772, 1839
SchI GAGTC 3 cut(s) 719, 737, 802
ScrFI CCNGG 2 cut(s) 757, 1295
SduI GDGCHC 1 cut(s) 1488
SfaNI GCATC 4 cut(s) 216, 271, 1002, 1089
Sfr274I CTCGAG 1 cut(s) 54
SfuI TTCGAA 2 cut(s) 372, 1058
SlaI CTCGAG 1 cut(s) 54
SmiMI CAYNNNNRTG 5 cut(s) 839, 936, 1082, 1715, 1766
SmlI CTYRAG 2 cut(s) 54, 230
SmoI CTYRAG 2 cut(s) 54, 230
SpeI ACTAGT 1 cut(s) 925
SphI GCATGC 1 cut(s) 267
SsiI CCGC 4 cut(s) 205, 433, 656, 1438
SspMI CTAG 2 cut(s) 926, 1571
StyD4I CCNGG 2 cut(s) 755, 1293
StyI CCWWGG 4 cut(s) 136, 181, 834, 1421
TaaI ACNGT 4 cut(s) 566, 738, 1473, 1602
TaiI ACGT 3 cut(s) 501, 702, 1514
TaqI TCGA 7 cut(s) 55, 61, 196, 315, 372, 708, 1058
TatI WGTACW 3 cut(s) 579, 1140, 1677
TauI GCSGC 1 cut(s) 1441
TfiI GAWTC 4 cut(s) 340, 492, 731, 1408
Tru1I TTAA 9 cut(s) 87, 176, 231, 333, 570, 641, 1181, 1476, 1766
Tru9I TTAA 9 cut(s) 87, 176, 231, 333, 570, 641, 1181, 1476, 1766
TscAI CASTG 9 cut(s) 78, 117, 454, 463, 571, 721, 1275, 1281, 1495
TseFI GTSAC 5 cut(s) 200, 380, 613, 1362, 1573
TseI GCWGC 4 cut(s) 37, 359, 1341, 1620
Tsp45I GTSAC 5 cut(s) 200, 380, 613, 1362, 1573
TspDTI ATGAA 5 cut(s) 463, 1216, 1407, 1540, 1677
TspRI CASTG 9 cut(s) 78, 117, 454, 463, 571, 721, 1275, 1281, 1495
Vha464I CTTAAG 1 cut(s) 230
XapI RAATTY 4 cut(s) 214, 527, 1218, 1395
XceI RCATGY 3 cut(s) 267, 1684, 1765
XhoI CTCGAG 1 cut(s) 54
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 2 cut(s) 926, 1571
ZraI GACGTC 1 cut(s) 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.