RchiOBHm_Chr2g0093381
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003988
Physical Location & Seq
Reverse (-)
6659914 .. 6662562
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46843

Sequence Viewer

Length: 1905 bp
ATGAGTAGCTTCTTCTTCTTCTTCGTCTTCGTTCTCCTCCTCTCTCTCTTGCCTTGTTCTCACTCATTCTATGCCACTGTACCCCGGAACAAGGGAGATACACTTGAAGTAGAGGTTAATCAGCTTTCATCAATCAGAACACAGCTTCCATATGACTACTATTTCCTCAGCTACTGCAAGCCTCCAGTAATTATCAACAGTTCATTCGGCCTTGGACAAGTTCTTGGGGGTGACCGCATCAAGAATTCGGTCTATTCTTTCAAGATGAGAGAGGCACAGTATTGCAAAGTTGCATGCCGTGTAAAACTGGATGCTAATTCTGCGAATAGTTTTAAGGACAAAATCGATGGCAACATGATTCTTGATGATCTCCCTGTTGCAGTTGCACTCAAAATGAGAAACTCTCGAGATGAGGGTCGATCAAAGATATATGAGCTTGGGTTCATGGTTGGGATTAAGGGACGTTATGCTGGAAACATTCTTGGTGCTTCTTCTTCACTGACACGGGAAAAAGAAGAAAGACATTTCATTCACAACGACCTGAGTTTTAAAGTCATGTATCATGAGGATCCAGAGACTGATTATACTCACATTGTCGGTTTTGAGGTCACACCGTACAGTATTAATCACGAGTACAAGGAGTGGAATGGTGAGAAGACTAAGCTTGTCACATGCCAGCCGAGCACCAATACTTACATTGAAGGTAGCAATAGTCCACAAGGGATTCATACTGATGAAGAAATTGTTTTCACATATGATGTATCTTTTGAGGAATCGCGTTGGGACTCATACCTCTACCTAGGTGATGATCCAATTCATTGGTTTGCTATGGTCAATTCATTGATAACTGTGCTCTTCCTTTCCGGAGTGGTAGCCATGGTTGTGATGAGAACTCTTTTCAAGGATATTACCAACTACAATCAGTTGGAGACCCAAGTTGAAGAAACAGGATGGAAACTGGTCCATGGAGATGTCTTCAGGCCACCCATCAATGCTAATATATTGTGTGTTTTTGTTGCGAATGGTGTCCAAGTCTTTGGTACAGTGCTTTTCACAATAACTCTTGCATTGCTGGGTTTCCTTTCACCTTCGAACCAAGGTGGGGAGATTAAAGCAATGGTTCTTTTGTGGTTGTTCATGGCCTTATTTGGGGGATACTACTCGGCGCACTTGTACAAGATGTTTAAAGGCATTCAGTGGAAGAAGATTGCATTAAAGACAGCCTTTATGTTCCCTGCTACATTTTTCGGAATATTTTTCATGTTGAATGTCTTCATTTGGTGGGAGGAGTCTTTTGCTGCAGTGCCGTTTGGTACAATGTTTTCAATGCTGCTCTTGTGGTTTGGGATATCAGTGCCATTAGAATTTGTAGGAAGTTACTTGGGGTTCAAACAGCAAGCAATTGAGGATCCAGTCAATACCAATGAAATCGCAAGACAAATACCAGTGCGAGCATGGTACAACATAAAGATGGTCTTGTCTGTGCTCATCGGAGGCATTCTACAGTTCGGAGTAGTGTTCGTTGAGTTCTTCTTTATGTGGACATCCATATGGATGAATAAGTTCTACTACTCTTTCGGGTTCCTCTTAATAGTCTTTGTCATCCTTCTAGTCACTTGTTGTGAGATAGTAATTCTACTCTGCTACTTCCAACTTCGCAGCGAAGATTACCATTGGCGGTGGAGAGCATACTTGACTGCCGGCTCCTCTTCTCTGTACATGTTTGCATACTCCATATTCCTTTTCTTCACCACGCTGTGGATTGAAAAGTTTGTTTCTGTCATCCTTTATTTTGGGTATATGTTAATTGCATCCTTTGCGTTCTTTGTCTTGACTGGGACAATCGGCTTCTATGCATGCTTCTGGTTTGTTAGGAAGATCTACTCCTCTGTCAAGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

634

Amino Acids

73.17

Weight (kDa)

6.29

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 50 - 591 6.6e-171 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1758
AccII CGCG 1 cut(s) 778
AccIII TCCGGA 1 cut(s) 863
AciI CCGC 2 cut(s) 235, 1678
AclWI GGATC 5 cut(s) 563, 576, 803, 1403, 1416
AcsI RAATTY 2 cut(s) 244, 1364
AcuI CTGAAG 1 cut(s) 961
AdeI CACNNNGTG 1 cut(s) 1758
AfaI GTAC 8 cut(s) 81, 617, 635, 1042, 1175, 1315, 1460, 1718
AfiI CCNNNNNNNGG 4 cut(s) 91, 1102, 1149, 1758
AflIII ACRYGT 1 cut(s) 1719
AgsI TTSAA 9 cut(s) 107, 262, 701, 901, 941, 1267, 1326, 1390, 1766
AloI GAACNNNNNNTCC 2 cut(s) 130, 162
AluBI AGCT 6 cut(s) 9, 124, 145, 171, 436, 664
AluI AGCT 6 cut(s) 9, 124, 145, 171, 436, 664
Alw21I GWGCWC 3 cut(s) 686, 855, 1488
Alw26I GTCTC 2 cut(s) 569, 923
AlwI GGATC 5 cut(s) 563, 576, 803, 1403, 1416
AlwNI CAGNNNCTG 2 cut(s) 174, 578
Ama87I CYCGRG 1 cut(s) 405
Aor13HI TCCGGA 1 cut(s) 863
AoxI GGCC 3 cut(s) 208, 980, 1140
ApeKI GCWGC 3 cut(s) 1298, 1330, 1659
ApoI RAATTY 2 cut(s) 244, 1364
AseI ATTAAT 1 cut(s) 624
Asp700I GAANNNNTTC 2 cut(s) 1271, 1562
AspA2I CCTAGG 1 cut(s) 799
AspLEI GCGC 1 cut(s) 1168
AspS9I GGNCC 1 cut(s) 961
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 5 cut(s) 242, 662, 815, 1077, 1741
AsuII TTCGAA 1 cut(s) 1091
AvaI CYCGRG 1 cut(s) 405
AvaII GGWCC 1 cut(s) 961
AvrII CCTAGG 1 cut(s) 799
BamHI GGATCC 2 cut(s) 568, 1408
BauI CACGAG 1 cut(s) 629
BbsI GAAGAC 4 cut(s) 19, 662, 967, 1264
Bbv12I GWGCWC 3 cut(s) 686, 855, 1488
BbvCI CCTCAGC 1 cut(s) 167
BbvI GCAGC 3 cut(s) 1285, 1317, 1671
BccI CCATC 4 cut(s) 341, 945, 995, 1465
BceAI ACGGC 2 cut(s) 282, 1291
BciVI GTATCC 1 cut(s) 1148
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 2 cut(s) 569, 923
BfaI CTAG 2 cut(s) 800, 1610
BfmI CTRYAG 2 cut(s) 1299, 1502
BfuI GTATCC 1 cut(s) 1148
BglII AGATCT 1 cut(s) 1878
BisI GCNGC 3 cut(s) 1299, 1331, 1660
BlnI CCTAGG 1 cut(s) 799
BlsI GCNGC 3 cut(s) 1300, 1332, 1661
Bme1390I CCNGG 1 cut(s) 85
Bme18I GGWCC 1 cut(s) 961
BmeT110I CYCGRG 1 cut(s) 405
BmgT120I GGNCC 1 cut(s) 961
BmiI GGNNCC 4 cut(s) 570, 1410, 1583, 1705
BmrFI CCNGG 1 cut(s) 85
BmrI ACTGGG 1 cut(s) 1845
BmsI GCATC 3 cut(s) 246, 301, 1820
BmuI ACTGGG 1 cut(s) 1845
BpiI GAAGAC 4 cut(s) 19, 662, 967, 1264
BplI GAGNNNNNCTC 2 cut(s) 388, 420
BpmI CTGGAG 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 167
Bpu14I TTCGAA 1 cut(s) 1091
BpuMI CCSGG 1 cut(s) 85
Bsa29I ATCGAT 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 923
BsaJI CCNNGG 6 cut(s) 83, 211, 799, 876, 964, 1096
BsaWI WCCGGW 1 cut(s) 863
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 1102, 1149, 1758
Bse118I RCCGGY 1 cut(s) 1700
Bse1I ACTGG 6 cut(s) 185, 312, 963, 1412, 1445, 1840
Bse3DI GCAATG 2 cut(s) 1067, 1122
BseAI TCCGGA 1 cut(s) 863
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 6 cut(s) 83, 211, 799, 876, 964, 1096
BseGI GGATG 7 cut(s) 316, 956, 1544, 1560, 1602, 1782, 1811
BseLI CCNNNNNNNGG 4 cut(s) 91, 1102, 1149, 1758
BseMI GCAATG 2 cut(s) 1067, 1122
BseMII CTCAG 2 cut(s) 181, 533
BseNI ACTGG 6 cut(s) 185, 312, 963, 1412, 1445, 1840
BseRI GAGGAG 5 cut(s) 26, 29, 1301, 1696, 1876
BseXI GCAGC 3 cut(s) 1285, 1317, 1671
BseYI CCCAGC 1 cut(s) 1072
Bsh1236I CGCG 1 cut(s) 778
BshFI GGCC 3 cut(s) 210, 982, 1142
BshVI ATCGAT 1 cut(s) 345
BsiHKAI GWGCWC 3 cut(s) 686, 855, 1488
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 3 cut(s) 85, 864, 1701
BslFI GGGAC 3 cut(s) 474, 797, 1852
BslI CCNNNNNNNGG 4 cut(s) 91, 1102, 1149, 1758
BsmAI GTCTC 2 cut(s) 569, 923
BsmFI GGGAC 3 cut(s) 474, 797, 1852
BsmI GAATGC 2 cut(s) 1191, 1497
BsnI GGCC 3 cut(s) 210, 982, 1142
Bso31I GGTCTC 1 cut(s) 923
BsoBI CYCGRG 1 cut(s) 405
Bsp119I TTCGAA 1 cut(s) 1091
Bsp1286I GDGCHC 3 cut(s) 686, 855, 1488
Bsp13I TCCGGA 1 cut(s) 863
Bsp1407I TGTACA 2 cut(s) 1173, 1716
Bsp143I GATC 6 cut(s) 367, 419, 568, 808, 1408, 1878
Bsp19I CCATGG 2 cut(s) 876, 964
BspACI CCGC 2 cut(s) 235, 1678
BspANI GGCC 3 cut(s) 210, 982, 1142
BspCNI CTCAG 2 cut(s) 180, 534
BspDI ATCGAT 1 cut(s) 345
BspEI TCCGGA 1 cut(s) 863
BspFNI CGCG 1 cut(s) 778
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 4 cut(s) 570, 1410, 1583, 1705
BspMAI CTGCAG 1 cut(s) 1303
BspPI GGATC 5 cut(s) 563, 576, 803, 1403, 1416
BspQI GCTCTTC 1 cut(s) 860
BspT104I TTCGAA 1 cut(s) 1091
BspTNI GGTCTC 1 cut(s) 923
BsrDI GCAATG 2 cut(s) 1067, 1122
BsrFI RCCGGY 1 cut(s) 1700
BsrGI TGTACA 2 cut(s) 1173, 1716
BsrI ACTGG 6 cut(s) 185, 312, 963, 1412, 1445, 1840
BssAI RCCGGY 1 cut(s) 1700
BssECI CCNNGG 6 cut(s) 83, 211, 799, 876, 964, 1096
BssMI GATC 6 cut(s) 367, 419, 568, 808, 1408, 1878
BssSI CACGAG 1 cut(s) 629
BssT1I CCWWGG 5 cut(s) 211, 799, 876, 964, 1096
Bst2BI CACGAG 1 cut(s) 629
Bst4CI ACNGT 8 cut(s) 79, 200, 279, 615, 620, 850, 1045, 1506
Bst6I CTCTTC 2 cut(s) 860, 1714
BstAPI GCANNNNNTGC 1 cut(s) 1817
BstAUI TGTACA 2 cut(s) 1173, 1716
BstBI TTCGAA 1 cut(s) 1091
BstC8I GCNNGC 7 cut(s) 179, 295, 677, 1398, 1452, 1702, 1858
BstDEI CTNAG 3 cut(s) 167, 542, 660
BstDSI CCRYGG 2 cut(s) 876, 964
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 7 cut(s) 316, 956, 1544, 1560, 1602, 1782, 1811
BstFNI CGCG 1 cut(s) 778
BstHHI GCGC 1 cut(s) 1168
BstKTI GATC 6 cut(s) 370, 422, 571, 811, 1411, 1881
BstMAI GTCTC 2 cut(s) 569, 923
BstMBI GATC 6 cut(s) 367, 419, 568, 808, 1408, 1878
BstMWI GCNNNNNNNGC 3 cut(s) 320, 681, 1817
BstNSI RCATGY 4 cut(s) 297, 675, 1723, 1860
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 2 cut(s) 1299, 1502
BstUI CGCG 1 cut(s) 778
BstV1I GCAGC 3 cut(s) 1285, 1317, 1671
BstV2I GAAGAC 4 cut(s) 19, 662, 967, 1264
BstX2I RGATCY 3 cut(s) 568, 1408, 1878
BstXI CCANNNNNNTGG 2 cut(s) 819, 1037
BstYI RGATCY 3 cut(s) 568, 1408, 1878
Bsu15I ATCGAT 1 cut(s) 345
BsuI GTATCC 1 cut(s) 1148
BsuRI GGCC 3 cut(s) 210, 982, 1142
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 2 cut(s) 876, 964
BtsCI GGATG 7 cut(s) 316, 956, 1544, 1560, 1602, 1782, 1811
BtsI GCAGTG 1 cut(s) 1308
BtsIMutI CAGTG 7 cut(s) 75, 497, 1050, 1202, 1308, 1359, 1452
Cac8I GCNNGC 7 cut(s) 179, 295, 677, 1398, 1452, 1702, 1858
CaiI CAGNNNCTG 2 cut(s) 174, 578
CciI TCATGA 1 cut(s) 562
CfoI GCGC 1 cut(s) 1168
Cfr10I RCCGGY 1 cut(s) 1700
Cfr13I GGNCC 1 cut(s) 961
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 8 cut(s) 80, 616, 634, 1041, 1174, 1314, 1459, 1717
CviQI GTAC 8 cut(s) 80, 616, 634, 1041, 1174, 1314, 1459, 1717
DdeI CTNAG 3 cut(s) 167, 542, 660
DpnI GATC 6 cut(s) 369, 421, 570, 810, 1410, 1880
DpnII GATC 6 cut(s) 367, 419, 568, 808, 1408, 1878
DraI TTTAAA 2 cut(s) 550, 1186
DraIII CACNNNGTG 1 cut(s) 1758
Eam1104I CTCTTC 2 cut(s) 860, 1714
EarI CTCTTC 2 cut(s) 860, 1714
Eco130I CCWWGG 5 cut(s) 211, 799, 876, 964, 1096
Eco31I GGTCTC 1 cut(s) 923
Eco32I GATATC 1 cut(s) 1350
Eco47I GGWCC 1 cut(s) 961
Eco57I CTGAAG 1 cut(s) 961
Eco88I CYCGRG 1 cut(s) 405
Eco91I GGTNACC 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 244
EcoRV GATATC 1 cut(s) 1350
EcoT14I CCWWGG 5 cut(s) 211, 799, 876, 964, 1096
EcoT22I ATGCAT 1 cut(s) 1858
ErhI CCWWGG 5 cut(s) 211, 799, 876, 964, 1096
FalI AAGNNNNNCTT 4 cut(s) 1208, 1240, 1460, 1492
FaqI GGGAC 3 cut(s) 474, 797, 1852
FauNDI CATATG 3 cut(s) 151, 754, 1550
Fnu4HI GCNGC 3 cut(s) 1299, 1331, 1660
FokI GGATG 7 cut(s) 323, 963, 1531, 1567, 1589, 1769, 1798
Fsp4HI GCNGC 3 cut(s) 1299, 1331, 1660
FspBI CTAG 2 cut(s) 800, 1610
GlaI GCGC 1 cut(s) 1167
GluI GCNGC 3 cut(s) 1299, 1331, 1660
GsaI CCCAGC 1 cut(s) 1076
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 3 cut(s) 210, 982, 1142
HapII CCGG 3 cut(s) 85, 864, 1701
HhaI GCGC 1 cut(s) 1168
Hin6I GCGC 1 cut(s) 1166
HinP1I GCGC 1 cut(s) 1166
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 5 cut(s) 358, 724, 773, 785, 1289
HpaII CCGG 3 cut(s) 85, 864, 1701
HphI GGTGA 5 cut(s) 242, 662, 815, 1077, 1741
Hpy166II GTNNAC 2 cut(s) 716, 1542
Hpy188I TCNGA 4 cut(s) 137, 1250, 1493, 1511
Hpy8I GTNNAC 2 cut(s) 716, 1542
HpyAV CCTTC 3 cut(s) 695, 1098, 1616
HpyCH4III ACNGT 8 cut(s) 79, 200, 279, 615, 620, 850, 1045, 1506
HpyCH4IV ACGT 1 cut(s) 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 681, 1817
HpyF3I CTNAG 3 cut(s) 167, 542, 660
HpySE526I ACGT 1 cut(s) 463
HspAI GCGC 1 cut(s) 1166
Kpn2I TCCGGA 1 cut(s) 863
KroI GCCGGC 1 cut(s) 1700
KroNI GCCGGC 1 cut(s) 1702
Kzo9I GATC 6 cut(s) 367, 419, 568, 808, 1408, 1878
LguI GCTCTTC 1 cut(s) 860
LmnI GCTCC 1 cut(s) 1709
Lsp1109I GCAGC 3 cut(s) 1285, 1317, 1671
LweI GCATC 3 cut(s) 246, 301, 1820
MaeI CTAG 2 cut(s) 800, 1610
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 5 cut(s) 230, 607, 667, 1376, 1612
MalI GATC 6 cut(s) 369, 421, 570, 810, 1410, 1880
MboI GATC 6 cut(s) 367, 419, 568, 808, 1408, 1878
MfeI CAATTG 1 cut(s) 1401
MflI RGATCY 3 cut(s) 568, 1408, 1878
MhlI GDGCHC 3 cut(s) 686, 855, 1488
MlyI GAGTC 2 cut(s) 779, 1298
MmeI TCCRAC 2 cut(s) 906, 1675
Mph1103I ATGCAT 1 cut(s) 1858
MroI TCCGGA 1 cut(s) 863
MroNI GCCGGC 1 cut(s) 1700
MroXI GAANNNNTTC 2 cut(s) 1271, 1562
MslI CAYNNNNRTG 6 cut(s) 732, 881, 969, 1469, 1549, 1553
MspI CCGG 3 cut(s) 85, 864, 1701
MspR9I CCNGG 1 cut(s) 85
MunI CAATTG 1 cut(s) 1401
Mva1269I GAATGC 2 cut(s) 1191, 1497
MvnI CGCG 1 cut(s) 778
MwoI GCNNNNNNNGC 3 cut(s) 320, 681, 1817
NaeI GCCGGC 1 cut(s) 1702
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 2 cut(s) 876, 964
NdeI CATATG 3 cut(s) 151, 754, 1550
NdeII GATC 6 cut(s) 367, 419, 568, 808, 1408, 1878
NgoMIV GCCGGC 1 cut(s) 1700
NlaIV GGNNCC 4 cut(s) 570, 1410, 1583, 1705
NmeAIII GCCGAG 2 cut(s) 705, 1142
NmuCI GTSAC 4 cut(s) 230, 607, 667, 1612
NsiI ATGCAT 1 cut(s) 1858
NspI RCATGY 4 cut(s) 297, 675, 1723, 1860
NspV TTCGAA 1 cut(s) 1091
PaeI GCATGC 2 cut(s) 297, 1860
PaeR7I CTCGAG 1 cut(s) 405
PagI TCATGA 1 cut(s) 562
PciI ACATGT 1 cut(s) 1719
PciSI GCTCTTC 1 cut(s) 860
PctI GAATGC 2 cut(s) 1191, 1497
PdiI GCCGGC 1 cut(s) 1702
PdmI GAANNNNTTC 2 cut(s) 1271, 1562
PfeI GAWTC 3 cut(s) 358, 724, 773
PflMI CCANNNNNTGG 1 cut(s) 1758
PkrI GCNGC 3 cut(s) 1300, 1332, 1661
PleI GAGTC 2 cut(s) 779, 1297
PpsI GAGTC 2 cut(s) 779, 1297
PscI ACATGT 1 cut(s) 1719
PshBI ATTAAT 1 cut(s) 624
PspEI GGTNACC 1 cut(s) 230
PspFI CCCAGC 1 cut(s) 1072
PspN4I GGNNCC 4 cut(s) 570, 1410, 1583, 1705
PspPI GGNCC 1 cut(s) 961
PstI CTGCAG 1 cut(s) 1303
PstNI CAGNNNCTG 2 cut(s) 174, 578
PsuI RGATCY 3 cut(s) 568, 1408, 1878
RsaI GTAC 8 cut(s) 81, 617, 635, 1042, 1175, 1315, 1460, 1718
RsaNI GTAC 8 cut(s) 80, 616, 634, 1041, 1174, 1314, 1459, 1717
RseI CAYNNNNRTG 6 cut(s) 732, 881, 969, 1469, 1549, 1553
SapI GCTCTTC 1 cut(s) 860
SatI GCNGC 3 cut(s) 1299, 1331, 1660
Sau3AI GATC 6 cut(s) 367, 419, 568, 808, 1408, 1878
Sau96I GGNCC 1 cut(s) 961
SchI GAGTC 2 cut(s) 779, 1298
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 3 cut(s) 686, 855, 1488
SfaNI GCATC 3 cut(s) 246, 301, 1820
SfcI CTRYAG 2 cut(s) 1299, 1502
Sfr274I CTCGAG 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 1091
SinI GGWCC 1 cut(s) 961
SlaI CTCGAG 1 cut(s) 405
SmiMI CAYNNNNRTG 6 cut(s) 732, 881, 969, 1469, 1549, 1553
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SphI GCATGC 2 cut(s) 297, 1860
SsiI CCGC 2 cut(s) 235, 1678
SspI AATATT 1 cut(s) 1254
SspMI CTAG 2 cut(s) 800, 1610
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 5 cut(s) 211, 799, 876, 964, 1096
TaaI ACNGT 8 cut(s) 79, 200, 279, 615, 620, 850, 1045, 1506
TaiI ACGT 1 cut(s) 466
TaqI TCGA 4 cut(s) 345, 406, 418, 1091
TaqII GACCGA 1 cut(s) 238
TatI WGTACW 3 cut(s) 633, 1173, 1716
TfiI GAWTC 3 cut(s) 358, 724, 773
TscAI CASTG 7 cut(s) 82, 504, 1050, 1202, 1308, 1359, 1452
TseFI GTSAC 4 cut(s) 230, 607, 667, 1612
TseI GCWGC 3 cut(s) 1298, 1330, 1659
Tsp45I GTSAC 4 cut(s) 230, 607, 667, 1612
TspRI CASTG 7 cut(s) 82, 504, 1050, 1202, 1308, 1359, 1452
Van91I CCANNNNNTGG 1 cut(s) 1758
VpaK11BI GGWCC 1 cut(s) 961
VspI ATTAAT 1 cut(s) 624
XapI RAATTY 2 cut(s) 244, 1364
XceI RCATGY 4 cut(s) 297, 675, 1723, 1860
XcmI CCANNNNNNNNNTGG 1 cut(s) 1452
XhoI CTCGAG 1 cut(s) 405
XmaJI CCTAGG 1 cut(s) 799
XmnI GAANNNNTTC 2 cut(s) 1271, 1562
XspI CTAG 2 cut(s) 800, 1610
Zsp2I ATGCAT 1 cut(s) 1858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.