Rh2AG081300
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003988
Physical Location & Seq
Reverse (-)
6813339 .. 6815129
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG081300.1

Sequence Viewer

Length: 1005 bp
ATGGGCAGCCTCTTCTTCTTCATCCTTCTACTTTCACTTGTTCCTCGGTCTCACTCTTTCTATGGCGATGTAGCTCGGGATTTTAAGAACAAGGAAGATACACTGGAAGTAAAGGTTAATAAGCTATCATCCATCAAGACACTGCTTCCCTATGACTACTATTCCCTTGGTTACTGCAAGCCTCCAGTAATCACCAATAGTGCTTTAAGCCTTGGAGAAGTTCTTCGAGGTGACCGCATCAAGAATTCAATCTACACTCTTAAGATGAGAGAGGCAGAATACTGCAAAGTCGCATGCCGTGTGAAACTGGATGCTAATTCTGCCAAAAAGTTTAGGGAGAAAATCGATGATCAATATGTAGTTAATATGATTCTTGATAATCTTCCTGCTGCAATTACATTCGAAGCGAGTGACTGGAATGGGAATAAATCAAAGATACCTGTAACTGCACTCCCAGTTGGGCGGAAGGGATATGCCACTGAAGGCAGTGAGGAAAGACACTTCATTTACAATCACCTGAGATTCAACGTCATGTACCATGAGGATCCGGAGACCAAATTTTCTCGCATTGTTGGTTTTGAGATTACACCATACAGTGTTAATCATGAGTACAAGAAGTGGGAAAATGAGAAAACTGAGTTGGTCACCTGCACGACCAATTCTGACCTTTTAATTAGCAATTTACCGCAAGAGATTGATACTGATAAAGAAGTTGTCTTTACATATGACGTATTGTTCGAGTCCAGTGATGTCAAGTGGGAATCGCGGTGGGACTCATACCTCTCCAGGGGTACTGATAATCAAATCCATTGGTTATCTATGTTCAACTCATTGTGGACTCTGCTCCTTCTTTCTGGAGTAATAGCCATGGTTGTGGTGCGAACTCTTTACAGGGATATCAACAGCTACAATCAGTTGGAGACCCAAGATGAGGCTCAGGAACTAACAGGATGGAAACTAGTCCATGCAGATGTTTTCAGGCCACCCCCCCCCCCCCAACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

334

Amino Acids

38.67

Weight (kDa)

5.88

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 50 - 330 1.2e-79 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 656
Acc36I ACCTGC 1 cut(s) 656
AccII CGCG 1 cut(s) 766
AccIII TCCGGA 1 cut(s) 547
AciI CCGC 4 cut(s) 235, 463, 686, 766
AclWI GGATC 2 cut(s) 539, 552
AcsI RAATTY 2 cut(s) 244, 557
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 3 cut(s) 536, 611, 793
AfiI CCNNNNNNNGG 2 cut(s) 787, 931
AflII CTTAAG 1 cut(s) 260
AgsI TTSAA 3 cut(s) 249, 526, 826
AhlI ACTAGT 1 cut(s) 958
AjnI CCWGG 1 cut(s) 785
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
AluBI AGCT 3 cut(s) 74, 124, 906
AluI AGCT 3 cut(s) 74, 124, 906
Alw26I GTCTC 3 cut(s) 54, 545, 914
AlwI GGATC 2 cut(s) 539, 552
Ama87I CYCGRG 1 cut(s) 75
Aor13HI TCCGGA 1 cut(s) 547
AoxI GGCC 1 cut(s) 980
ApeKI GCWGC 2 cut(s) 6, 389
ApoI RAATTY 2 cut(s) 244, 557
ArsI GACNNNNNNTTYG 2 cut(s) 719, 751
Asp700I GAANNNNTTC 1 cut(s) 222
AsuHPI GGTGA 4 cut(s) 184, 242, 506, 637
AsuII TTCGAA 1 cut(s) 402
AvaI CYCGRG 1 cut(s) 75
BamHI GGATCC 1 cut(s) 544
BbvI GCAGC 2 cut(s) 18, 376
BccI CCATC 2 cut(s) 140, 945
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 1 cut(s) 787
BclI TGATCA 1 cut(s) 349
BcoDI GTCTC 3 cut(s) 54, 545, 914
BcuI ACTAGT 1 cut(s) 958
BfaI CTAG 1 cut(s) 959
BfrI CTTAAG 1 cut(s) 260
BfuAI ACCTGC 1 cut(s) 656
BisI GCNGC 2 cut(s) 7, 390
BlsI GCNGC 2 cut(s) 8, 391
Bme1390I CCNGG 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 546
BmrFI CCNGG 1 cut(s) 787
BmrI ACTGGG 1 cut(s) 449
BmsI GCATC 2 cut(s) 246, 301
BmuI ACTGGG 1 cut(s) 449
BpmI CTGGAG 3 cut(s) 168, 769, 876
Bpu10I CCTNAGC 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 402
Bsa29I ATCGAT 1 cut(s) 345
BsaI GGTCTC 3 cut(s) 54, 545, 914
BsaJI CCNNGG 5 cut(s) 44, 166, 211, 786, 867
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 2 cut(s) 787, 931
Bse1I ACTGG 6 cut(s) 108, 185, 312, 419, 455, 744
BseAI TCCGGA 1 cut(s) 547
BseBI CCWGG 1 cut(s) 787
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 5 cut(s) 44, 166, 211, 786, 867
BseGI GGATG 4 cut(s) 21, 128, 316, 956
BseLI CCNNNNNNNGG 2 cut(s) 787, 931
BseMII CTCAG 3 cut(s) 509, 627, 950
BseNI ACTGG 6 cut(s) 108, 185, 312, 419, 455, 744
BseXI GCAGC 2 cut(s) 18, 376
BsgI GTGCAG 2 cut(s) 432, 634
Bsh1236I CGCG 1 cut(s) 766
BshFI GGCC 1 cut(s) 982
BshVI ATCGAT 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 1 cut(s) 548
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 2 cut(s) 787, 931
BsmAI GTCTC 3 cut(s) 54, 545, 914
BsmFI GGGAC 1 cut(s) 785
BsnI GGCC 1 cut(s) 982
Bso31I GGTCTC 3 cut(s) 54, 545, 914
BsoBI CYCGRG 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 402
Bsp13I TCCGGA 1 cut(s) 547
Bsp143I GATC 2 cut(s) 349, 544
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 4 cut(s) 235, 463, 686, 766
BspANI GGCC 1 cut(s) 982
BspCNI CTCAG 3 cut(s) 510, 628, 949
BspDI ATCGAT 1 cut(s) 345
BspEI TCCGGA 1 cut(s) 547
BspFNI CGCG 1 cut(s) 766
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 1 cut(s) 546
BspMI ACCTGC 1 cut(s) 656
BspPI GGATC 2 cut(s) 539, 552
BspT104I TTCGAA 1 cut(s) 402
BspTI CTTAAG 1 cut(s) 260
BspTNI GGTCTC 3 cut(s) 54, 545, 914
BsrI ACTGG 6 cut(s) 108, 185, 312, 419, 455, 744
BssECI CCNNGG 5 cut(s) 44, 166, 211, 786, 867
BssMI GATC 2 cut(s) 349, 544
BssT1I CCWWGG 3 cut(s) 166, 211, 867
Bst2UI CCWGG 1 cut(s) 787
Bst4CI ACNGT 1 cut(s) 596
Bst6I CTCTTC 1 cut(s) 17
BstAFI CTTAAG 1 cut(s) 260
BstBI TTCGAA 1 cut(s) 402
BstC8I GCNNGC 2 cut(s) 179, 295
BstDEI CTNAG 3 cut(s) 518, 636, 936
BstDSI CCRYGG 1 cut(s) 867
BstEII GGTNACC 2 cut(s) 230, 643
BstF5I GGATG 4 cut(s) 21, 128, 316, 956
BstFNI CGCG 1 cut(s) 766
BstKTI GATC 2 cut(s) 352, 547
BstMAI GTCTC 3 cut(s) 54, 545, 914
BstMBI GATC 2 cut(s) 349, 544
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNI CCWGG 1 cut(s) 787
BstNSI RCATGY 1 cut(s) 297
BstPI GGTNACC 2 cut(s) 230, 643
BstSCI CCNGG 1 cut(s) 785
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 2 cut(s) 18, 376
BstX2I RGATCY 1 cut(s) 544
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 1 cut(s) 544
Bsu15I ATCGAT 1 cut(s) 345
BsuRI GGCC 1 cut(s) 982
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 1 cut(s) 867
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 4 cut(s) 21, 128, 316, 956
BtsI GCAGTG 2 cut(s) 140, 493
BtsIMutI CAGTG 6 cut(s) 101, 140, 477, 493, 601, 751
BveI ACCTGC 1 cut(s) 656
Cac8I GCNNGC 2 cut(s) 179, 295
CciI TCATGA 1 cut(s) 604
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 3 cut(s) 535, 610, 792
CviAII CATG 6 cut(s) 294, 532, 539, 605, 868, 965
CviJI RGCY 9 cut(s) 9, 74, 124, 181, 210, 866, 906, 935, 982
CviKI_1 RGCY 9 cut(s) 9, 74, 124, 181, 210, 866, 906, 935, 982
CviQI GTAC 3 cut(s) 535, 610, 792
DdeI CTNAG 3 cut(s) 518, 636, 936
DpnI GATC 2 cut(s) 351, 546
DpnII GATC 2 cut(s) 349, 544
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
EciI GGCGGA 1 cut(s) 478
Eco130I CCWWGG 3 cut(s) 166, 211, 867
Eco31I GGTCTC 3 cut(s) 54, 545, 914
Eco32I GATATC 1 cut(s) 898
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 75
Eco91I GGTNACC 2 cut(s) 230, 643
EcoO65I GGTNACC 2 cut(s) 230, 643
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 785
EcoRV GATATC 1 cut(s) 898
EcoT14I CCWWGG 3 cut(s) 166, 211, 867
ErhI CCWWGG 3 cut(s) 166, 211, 867
FaeI CATG 6 cut(s) 297, 535, 542, 608, 871, 968
FaqI GGGAC 1 cut(s) 785
FatI CATG 6 cut(s) 293, 531, 538, 604, 867, 964
FauNDI CATATG 1 cut(s) 724
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 7, 390
FokI GGATG 4 cut(s) 8, 115, 323, 963
Fsp4HI GCNGC 2 cut(s) 7, 390
FspBI CTAG 1 cut(s) 959
GluI GCNGC 2 cut(s) 7, 390
GsuI CTGGAG 3 cut(s) 168, 769, 876
HaeIII GGCC 1 cut(s) 982
HapII CCGG 1 cut(s) 548
Hin1II CATG 6 cut(s) 297, 535, 542, 608, 871, 968
HinfI GANTC 6 cut(s) 370, 522, 740, 761, 773, 838
HpaII CCGG 1 cut(s) 548
HphI GGTGA 4 cut(s) 184, 242, 506, 637
Hpy166II GTNNAC 1 cut(s) 837
Hpy188I TCNGA 1 cut(s) 664
Hpy188III TCNNGA 8 cut(s) 77, 136, 241, 374, 548, 605, 855, 938
Hpy8I GTNNAC 1 cut(s) 837
HpyAV CCTTC 4 cut(s) 35, 460, 476, 857
HpyCH4III ACNGT 1 cut(s) 596
HpyCH4IV ACGT 2 cut(s) 528, 729
HpyCH4V TGCA 6 cut(s) 177, 285, 392, 449, 651, 968
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 3 cut(s) 518, 636, 936
HpySE526I ACGT 2 cut(s) 528, 729
Hsp92II CATG 6 cut(s) 297, 535, 542, 608, 871, 968
Kpn2I TCCGGA 1 cut(s) 547
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 349, 544
LmnI GCTCC 1 cut(s) 849
Lsp1109I GCAGC 2 cut(s) 18, 376
LweI GCATC 2 cut(s) 246, 301
MaeI CTAG 1 cut(s) 959
MaeII ACGT 2 cut(s) 528, 729
MaeIII GTNAC 5 cut(s) 170, 230, 410, 442, 643
MalI GATC 2 cut(s) 351, 546
MboI GATC 2 cut(s) 349, 544
MboII GAAGA 6 cut(s) 4, 7, 10, 107, 215, 374
MflI RGATCY 1 cut(s) 544
MluCI AATT 7 cut(s) 244, 316, 393, 557, 658, 672, 679
MlyI GAGTC 3 cut(s) 749, 767, 832
MmeI TCCRAC 1 cut(s) 897
MnlI CCTC 9 cut(s) 20, 54, 192, 221, 265, 484, 535, 791, 925
MroI TCCGGA 1 cut(s) 547
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 7 cut(s) 84, 117, 206, 261, 363, 600, 671
MslI CAYNNNNRTG 2 cut(s) 872, 969
MspCI CTTAAG 1 cut(s) 260
MspI CCGG 1 cut(s) 548
MspR9I CCNGG 1 cut(s) 787
MvaI CCWGG 1 cut(s) 787
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 1 cut(s) 320
NcoI CCATGG 1 cut(s) 867
NdeI CATATG 1 cut(s) 724
NdeII GATC 2 cut(s) 349, 544
NlaIII CATG 6 cut(s) 297, 535, 542, 608, 871, 968
NlaIV GGNNCC 1 cut(s) 546
NmuCI GTSAC 3 cut(s) 230, 410, 643
NspI RCATGY 1 cut(s) 297
NspV TTCGAA 1 cut(s) 402
PaeI GCATGC 1 cut(s) 297
PagI TCATGA 1 cut(s) 604
PaqCI CACCTGC 1 cut(s) 656
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 3 cut(s) 370, 522, 761
PkrI GCNGC 2 cut(s) 8, 391
PleI GAGTC 3 cut(s) 748, 767, 832
PpsI GAGTC 3 cut(s) 748, 767, 832
Psp6I CCWGG 1 cut(s) 785
PspEI GGTNACC 2 cut(s) 230, 643
PspGI CCWGG 1 cut(s) 785
PspN4I GGNNCC 1 cut(s) 546
PsuI RGATCY 1 cut(s) 544
RsaI GTAC 3 cut(s) 536, 611, 793
RsaNI GTAC 3 cut(s) 535, 610, 792
RseI CAYNNNNRTG 2 cut(s) 872, 969
SaqAI TTAA 7 cut(s) 84, 117, 206, 261, 363, 600, 671
SatI GCNGC 2 cut(s) 7, 390
Sau3AI GATC 2 cut(s) 349, 544
SchI GAGTC 3 cut(s) 749, 767, 832
ScrFI CCNGG 1 cut(s) 787
SfaNI GCATC 2 cut(s) 246, 301
SfuI TTCGAA 1 cut(s) 402
SmiMI CAYNNNNRTG 2 cut(s) 872, 969
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SpeI ACTAGT 1 cut(s) 958
SphI GCATGC 1 cut(s) 297
Sse9I AATT 7 cut(s) 244, 316, 393, 557, 658, 672, 679
SsiI CCGC 4 cut(s) 235, 463, 686, 766
SspMI CTAG 1 cut(s) 959
StyD4I CCNGG 1 cut(s) 785
StyI CCWWGG 3 cut(s) 166, 211, 867
TaaI ACNGT 1 cut(s) 596
TaiI ACGT 2 cut(s) 531, 732
TaqI TCGA 4 cut(s) 226, 345, 402, 738
TaqII GACCGA 1 cut(s) 36
TasI AATT 7 cut(s) 244, 316, 393, 557, 658, 672, 679
TatI WGTACW 1 cut(s) 609
TfiI GAWTC 3 cut(s) 370, 522, 761
Tru1I TTAA 7 cut(s) 84, 117, 206, 261, 363, 600, 671
Tru9I TTAA 7 cut(s) 84, 117, 206, 261, 363, 600, 671
TscAI CASTG 6 cut(s) 108, 147, 484, 493, 601, 751
TseFI GTSAC 3 cut(s) 230, 410, 643
TseI GCWGC 2 cut(s) 6, 389
Tsp45I GTSAC 3 cut(s) 230, 410, 643
TspDTI ATGAA 2 cut(s) 10, 493
TspRI CASTG 6 cut(s) 108, 147, 484, 493, 601, 751
Vha464I CTTAAG 1 cut(s) 260
XapI RAATTY 2 cut(s) 244, 557
XceI RCATGY 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 1 cut(s) 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.