RchiOBHm_Chr2g0103231

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006338
Physical Location & Seq
Reverse (-)
14805780 .. 14809044
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47759

Sequence Viewer

Length: 2019 bp
ATGGAGATAACAGAAGCAGCTGAGAAACCAAGCTCCAAAGGCCAAGCATGGTTCTGCACCACTGGGTTACCAAGCGACATTGTGGTGGAAGTTGAAGACATGTCCTTTCATCTTCACAAGTTTCCTCTCATGTCCAAAAGCCGAAAGCTTCACAACCTAATCACGGAGCAAGAGGCTAACCCATCACATCCCCAACAAGAGCTAGAAGAAGAGCCGGACGAAATTGAAGAAGTGCAGTGTCAAATAGTCCTCTCAGACTTCCCGGGCGGCTCCGAGACCTTCGAAATCGCCGCCAAATTCTGCTACGGCGTCAAAATCGACCTCAACTCGACCAATGCGGGACCTCTCCGCTGCGCCGGAGAGTATCTAGAAATGACAGAAGAGTACTCCGAAGACAACCTCATCTCAAAAACCGAAAGGTTCCTGTCTCAAGCCGTGTTCAAGAGCTTCAAAGAGTCCATCAAAGCTCTCAAATCCTGCGAGCGCGTCATGCCACTGGCAGAGACCCTAGGCATTACACAGAGGTGCATCGACTCCATCGCTAACAGAGCCACCAACGCCGATCCGAGCCTGTTCGGCTGGCCCGTCAATGAATTCGCCCCGAACCCAAGTTCATCCAAACACCAGATTCTATGGAATGGGATTGACACAGGTGGAAGAAGAAAAGGGATGTCTAGAGCAAACCATCCAGATTCTTGGCTCGAAGACTTGGTCCTCTTAAGCCTCCCTTTCTTCAAGCGATTGATTATGGCAATGAAAGACGGAAATCTCAGCCTGGAAATGGTGGAGACCTGTTTGATGTACTACGCCAAGAAATACATTCCCGGAATCTCAAGATCGACTCGAAAGTCCTCTTCTTCCACCACCACTTCTAGCTCCTCTTTAGCTTTGGAAAACCAACAGAGAGAACTATTGGAGACCATAATCTCAAACCTTCCATTGGAGAAAACACCAAGACCTCCAACCAATACAAGATTTCTATTTGGGCTGTTGAGAACAGCGAATATCTTAAACGCCTCGCAGGGATGTAAAGCCGCATTGGAGAAGAAGATTGGGTCGCAGCTCGAGCAAGCTAGCTTAGACGATCTGCTCATACCGAGCTATTCGTATCTGAATGAGACCCTATACGACGTCGATTGCGTGGAGAGGATTCTGGGGTATTTTTTGGACGGGTTGGAGCAGAGAAACAGAAACAGAGATGGAATTGAAGGTGGAGATGAAGACGAAGACCGTGATATAGAAGGTGCTAGATCGCCGGCGCTGATGCTTGTCGGGAAATTAACCGACGAGTATCTCTCCGAGATCGCATCGGATGCGAATTTGATGCCGGATAGATTCTACAACTTTGCCATGGCGCTGCCGGAGCAGGCGAGGCTGTTCGACGACGGGCTTTACAGAGCAGTTGATGTTTATCTAAAGGCTCATCCATGGATATCGGAACCGGAGCGGGAGAAGATCTGCGGGATACTAGACTCCCAGAAGCTGACACTCGAGGCGTGCACGCACGCCGCGCAGAACGAGCGGTTGCCGCTACGCGCCGTCGTGCAAGTCTTGTTCTTCGAGCAGCTTCAGCTCCGTCACGCGATCGCCGGTACCCTTATCGCCGGGGAGACCGCGCCGCCGGACTCCAGTCGGCCCTCGGTGTTGCGAAGAGAGGCGGAGACTGAGGAGGACGAGGAGCAGGAGGAGGAGGAGGAGGAGGAAGGCGGTGCTGTGGAGGTACACGCGGAGGAAGGAGGAGGCGGCACGTGGAGGTCGGCTGTGAGGGAGAATCAGGTGCTGCGGTTGGATATGGATAGCATGAGGACACGGGTGCACCAACTTGAGCGGGAGTGTTCTAACATGAAGAAGGTGATCGAAAAGATTGACAACAAATCGGGTCAACCCGATGGACGGGCCGGATGGAAAGGCTCGTTGACCCGGCGGTTCGGGTGCAAGTTCAAGACCCAAGTGTGCGATTCGCACGAGCCGACGGTCGTGGACGGGCGGAAAGGGCGGCGCCACCAGCATCAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

672

Amino Acids

75.6

Weight (kDa)

5.04

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 231 - 500 9.3e-86 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 847
AatII GACGTC 1 cut(s) 1134
Acc65I GGTACC 1 cut(s) 1592
AccB1I GGYRCC 2 cut(s) 1592, 1998
AccBSI CCGCTC 3 cut(s) 1447, 1522, 1828
AccII CGCG 6 cut(s) 486, 1511, 1536, 1583, 1616, 1727
AclWI GGATC 1 cut(s) 557
AcsI RAATTY 3 cut(s) 296, 593, 1318
AcuI CTGAAG 1 cut(s) 1553
AcvI CACGTG 1 cut(s) 1749
AcyI GRCGYC 3 cut(s) 309, 1131, 1999
AfaI GTAC 4 cut(s) 386, 803, 1594, 1722
AfiI CCNNNNNNNGG 4 cut(s) 163, 781, 940, 1893
AflII CTTAAG 1 cut(s) 718
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 7 cut(s) 95, 227, 442, 451, 736, 1208, 1942
AjnI CCWGG 1 cut(s) 774
AleI CACNNNNGTG 1 cut(s) 523
Alw21I GWGCWC 2 cut(s) 1502, 1818
Alw26I GTCTC 8 cut(s) 269, 432, 497, 782, 911, 1112, 1604, 1655
Alw44I GTGCAC 2 cut(s) 1498, 1814
AlwI GGATC 1 cut(s) 557
AlwNI CAGNNNCTG 2 cut(s) 1483, 1780
Ama87I CYCGRG 3 cut(s) 262, 1064, 1490
AoxI GGCC 4 cut(s) 40, 581, 1634, 1896
ApaLI GTGCAC 2 cut(s) 1498, 1814
ApeKI GCWGC 6 cut(s) 17, 351, 1060, 1357, 1564, 1780
ApoI RAATTY 3 cut(s) 296, 593, 1318
ArsI GACNNNNNNTTYG 2 cut(s) 696, 728
AsiSI GCGATCGC 1 cut(s) 1587
Asp718I GGTACC 1 cut(s) 1592
AspA2I CCTAGG 1 cut(s) 508
AspLEI GCGC 8 cut(s) 356, 486, 1261, 1357, 1513, 1538, 1618, 2001
AspS9I GGNCC 5 cut(s) 341, 582, 712, 1635, 1896
AsuC2I CCSGG 5 cut(s) 263, 264, 825, 1606, 1921
AsuHPI GGTGA 1 cut(s) 1864
AsuII TTCGAA 1 cut(s) 282
AsuNHI GCTAGC 1 cut(s) 1073
AvaI CYCGRG 3 cut(s) 262, 1064, 1490
AvaII GGWCC 2 cut(s) 341, 712
AvrII CCTAGG 1 cut(s) 508
BaeGI GKGCMC 2 cut(s) 1502, 1818
BanI GGYRCC 2 cut(s) 1592, 1998
BauI CACGAG 1 cut(s) 1964
BbrPI CACGTG 1 cut(s) 1749
BbsI GAAGAC 5 cut(s) 102, 399, 711, 1227, 1233
Bbv12I GWGCWC 2 cut(s) 1502, 1818
BbvI GCAGC 6 cut(s) 29, 338, 1072, 1344, 1576, 1767
BccI CCATC 7 cut(s) 190, 467, 545, 693, 1193, 1883, 1896
BceAI ACGGC 3 cut(s) 322, 419, 1523
BcgI CGANNNNNNTGC 2 cut(s) 1306, 1340
BciT130I CCWGG 1 cut(s) 776
BciVI GTATCC 1 cut(s) 1458
BcnI CCSGG 5 cut(s) 263, 264, 825, 1606, 1921
BcoDI GTCTC 8 cut(s) 269, 432, 497, 782, 911, 1112, 1604, 1655
BfaI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
BfoI RGCGCY 3 cut(s) 1262, 1358, 2002
BfrI CTTAAG 1 cut(s) 718
BfuI GTATCC 1 cut(s) 1458
BglI GCCNNNNNGGC 1 cut(s) 576
BglII AGATCT 1 cut(s) 1455
BlnI CCTAGG 1 cut(s) 508
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1921
Bme18I GGWCC 2 cut(s) 341, 712
BmeT110I CYCGRG 3 cut(s) 262, 1064, 1490
BmgT120I GGNCC 5 cut(s) 341, 582, 712, 1635, 1896
BmiI GGNNCC 6 cut(s) 271, 342, 422, 1440, 1594, 2000
BmrFI CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1921
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 5 cut(s) 537, 1254, 1303, 1314, 1316
BmtI GCTAGC 1 cut(s) 1077
BmuI ACTGGG 1 cut(s) 72
BoxI GACNNNNGTC 1 cut(s) 1629
BpiI GAAGAC 5 cut(s) 102, 399, 711, 1227, 1233
BplI GAGNNNNNCTC 2 cut(s) 1280, 1312
BpmI CTGGAG 1 cut(s) 1612
Bpu14I TTCGAA 1 cut(s) 282
BpuEI CTTGAG 3 cut(s) 414, 817, 1844
BpuMI CCSGG 5 cut(s) 263, 264, 825, 1606, 1921
BsaAI YACGTR 1 cut(s) 1749
BsaBI GATNNNNATC 1 cut(s) 1410
BsaHI GRCGYC 3 cut(s) 309, 1131, 1999
BsaI GGTCTC 6 cut(s) 269, 497, 782, 911, 1112, 1604
BsaJI CCNNGG 6 cut(s) 262, 508, 1350, 1427, 1605, 1638
BsaWI WCCGGW 1 cut(s) 1441
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 781, 940, 1893
Bse118I RCCGGY 2 cut(s) 1255, 1589
Bse1I ACTGG 3 cut(s) 67, 501, 1629
Bse3DI GCAATG 1 cut(s) 759
Bse8I GATNNNNATC 1 cut(s) 1410
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 6 cut(s) 262, 508, 1350, 1427, 1605, 1638
BseGI GGATG 8 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1907
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 4 cut(s) 163, 781, 940, 1893
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 4 cut(s) 12, 267, 784, 1656
BseNI ACTGG 3 cut(s) 67, 501, 1629
BseRI GAGGAG 9 cut(s) 868, 1682, 1691, 1700, 1703, 1706, 1709, 1712, 1752
BseSI GKGCMC 2 cut(s) 1502, 1818
BseXI GCAGC 6 cut(s) 29, 338, 1072, 1344, 1576, 1767
BsgI GTGCAG 2 cut(s) 40, 254
Bsh1236I CGCG 6 cut(s) 486, 1511, 1536, 1583, 1616, 1727
Bsh1285I CGRYCG 2 cut(s) 1587, 1977
BshFI GGCC 4 cut(s) 42, 583, 1636, 1898
BshNI GGYRCC 2 cut(s) 1592, 1998
BsiEI CGRYCG 2 cut(s) 1587, 1977
BsiHKAI GWGCWC 2 cut(s) 1502, 1818
BsiHKCI CYCGRG 3 cut(s) 262, 1064, 1490
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 4 cut(s) 163, 781, 940, 1893
BsmAI GTCTC 8 cut(s) 269, 432, 497, 782, 911, 1112, 1604, 1655
BsmFI GGGAC 1 cut(s) 354
BsnI GGCC 4 cut(s) 42, 583, 1636, 1898
Bso31I GGTCTC 6 cut(s) 269, 497, 782, 911, 1112, 1604
BsoBI CYCGRG 3 cut(s) 262, 1064, 1490
Bsp119I TTCGAA 1 cut(s) 282
Bsp1286I GDGCHC 2 cut(s) 1502, 1818
Bsp143I GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1854
Bsp19I CCATGG 2 cut(s) 1350, 1427
BspANI GGCC 4 cut(s) 42, 583, 1636, 1898
BspCNI CTCAG 4 cut(s) 13, 266, 783, 1657
BspFNI CGCG 6 cut(s) 486, 1511, 1536, 1583, 1616, 1727
BspLI GGNNCC 6 cut(s) 271, 342, 422, 1440, 1594, 2000
BspOI GCTAGC 1 cut(s) 1077
BspPI GGATC 1 cut(s) 557
BspQI GCTCTTC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 282
BspT107I GGYRCC 2 cut(s) 1592, 1998
BspTI CTTAAG 1 cut(s) 718
BspTNI GGTCTC 6 cut(s) 269, 497, 782, 911, 1112, 1604
BsrBI CCGCTC 3 cut(s) 1447, 1522, 1828
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 2 cut(s) 1255, 1589
BsrI ACTGG 3 cut(s) 67, 501, 1629
BssAI RCCGGY 2 cut(s) 1255, 1589
BssECI CCNNGG 6 cut(s) 262, 508, 1350, 1427, 1605, 1638
BssMI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1854
BssNI GRCGYC 3 cut(s) 309, 1131, 1999
BssSI CACGAG 1 cut(s) 1964
BssT1I CCWWGG 3 cut(s) 508, 1350, 1427
Bst2BI CACGAG 1 cut(s) 1964
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 2 cut(s) 1232, 1975
Bst6I CTCTTC 4 cut(s) 204, 375, 859, 1645
BstACI GRCGYC 3 cut(s) 309, 1131, 1999
BstAFI CTTAAG 1 cut(s) 718
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBAI YACGTR 1 cut(s) 1749
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 9 cut(s) 482, 581, 1071, 1075, 1257, 1368, 1498, 1502, 1506
BstDEI CTNAG 5 cut(s) 21, 253, 770, 1078, 1665
BstDSI CCRYGG 2 cut(s) 1350, 1427
BstEII GGTNACC 1 cut(s) 66
BstF5I GGATG 8 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1907
BstFNI CGCG 6 cut(s) 486, 1511, 1536, 1583, 1616, 1727
BstH2I RGCGCY 3 cut(s) 1262, 1358, 2002
BstHHI GCGC 8 cut(s) 356, 486, 1261, 1357, 1513, 1538, 1618, 2001
BstKTI GATC 8 cut(s) 565, 839, 1087, 1253, 1305, 1458, 1587, 1857
BstMAI GTCTC 8 cut(s) 269, 432, 497, 782, 911, 1112, 1604, 1655
BstMBI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1854
BstMCI CGRYCG 2 cut(s) 1587, 1977
BstNI CCWGG 1 cut(s) 776
BstNSI RCATGY 1 cut(s) 103
BstPAI GACNNNNGTC 1 cut(s) 1629
BstPI GGTNACC 1 cut(s) 66
BstSCI CCNGG 6 cut(s) 261, 262, 774, 823, 1604, 1919
BstSLI GKGCMC 2 cut(s) 1502, 1818
BstUI CGCG 6 cut(s) 486, 1511, 1536, 1583, 1616, 1727
BstV1I GCAGC 6 cut(s) 29, 338, 1072, 1344, 1576, 1767
BstV2I GAAGAC 5 cut(s) 102, 399, 711, 1227, 1233
BstX2I RGATCY 1 cut(s) 1455
BstXI CCANNNNNNTGG 1 cut(s) 696
BstYI RGATCY 1 cut(s) 1455
BsuI GTATCC 1 cut(s) 1458
BsuRI GGCC 4 cut(s) 42, 583, 1636, 1898
BtgI CCRYGG 2 cut(s) 1350, 1427
BtgZI GCGATG 1 cut(s) 523
BtsCI GGATG 8 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1907
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 3 cut(s) 60, 242, 494
Cac8I GCNNGC 9 cut(s) 482, 581, 1071, 1075, 1257, 1368, 1498, 1502, 1506
CaiI CAGNNNCTG 2 cut(s) 1483, 1780
CfoI GCGC 8 cut(s) 356, 486, 1261, 1357, 1513, 1538, 1618, 2001
Cfr10I RCCGGY 2 cut(s) 1255, 1589
Cfr13I GGNCC 5 cut(s) 341, 582, 712, 1635, 1896
Cfr9I CCCGGG 1 cut(s) 262
CseI GACGC 2 cut(s) 298, 475
Csp6I GTAC 4 cut(s) 385, 802, 1593, 1721
CviAII CATG 8 cut(s) 48, 100, 130, 490, 1351, 1428, 1801, 1843
CviQI GTAC 4 cut(s) 385, 802, 1593, 1721
DdeI CTNAG 5 cut(s) 21, 253, 770, 1078, 1665
DinI GGCGCC 1 cut(s) 2000
DpnI GATC 8 cut(s) 564, 838, 1086, 1252, 1304, 1457, 1586, 1856
DpnII GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1854
DrdI GACNNNNNNGTC 1 cut(s) 847
DseDI GACNNNNNNGTC 1 cut(s) 847
Eam1104I CTCTTC 4 cut(s) 204, 375, 859, 1645
EarI CTCTTC 4 cut(s) 204, 375, 859, 1645
EciI GGCGGA 2 cut(s) 1673, 2002
Eco130I CCWWGG 3 cut(s) 508, 1350, 1427
Eco31I GGTCTC 6 cut(s) 269, 497, 782, 911, 1112, 1604
Eco32I GATATC 1 cut(s) 1434
Eco47I GGWCC 2 cut(s) 341, 712
Eco57I CTGAAG 1 cut(s) 1553
Eco72I CACGTG 1 cut(s) 1749
Eco88I CYCGRG 3 cut(s) 262, 1064, 1490
Eco91I GGTNACC 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 66
EcoRI GAATTC 1 cut(s) 593
EcoRII CCWGG 1 cut(s) 774
EcoRV GATATC 1 cut(s) 1434
EcoT14I CCWWGG 3 cut(s) 508, 1350, 1427
EgeI GGCGCC 1 cut(s) 2000
EheI GGCGCC 1 cut(s) 2000
ErhI CCWWGG 3 cut(s) 508, 1350, 1427
FaeI CATG 8 cut(s) 51, 103, 133, 493, 1354, 1431, 1804, 1846
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FaqI GGGAC 1 cut(s) 354
FatI CATG 8 cut(s) 47, 99, 129, 489, 1350, 1427, 1800, 1842
FauI CCCGC 4 cut(s) 331, 1440, 1454, 1821
FokI GGATG 8 cut(s) 174, 601, 672, 682, 1038, 1325, 1410, 1914
FspBI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
GlaI GCGC 8 cut(s) 355, 485, 1260, 1356, 1512, 1537, 1617, 2000
GsuI CTGGAG 1 cut(s) 1612
HaeII RGCGCY 3 cut(s) 1262, 1358, 2002
HaeIII GGCC 4 cut(s) 42, 583, 1636, 1898
HgaI GACGC 2 cut(s) 298, 475
HhaI GCGC 8 cut(s) 356, 486, 1261, 1357, 1513, 1538, 1618, 2001
Hin1I GRCGYC 3 cut(s) 309, 1131, 1999
Hin1II CATG 8 cut(s) 51, 103, 133, 493, 1354, 1431, 1804, 1846
Hin6I GCGC 8 cut(s) 354, 484, 1259, 1355, 1511, 1536, 1616, 1999
HinP1I GCGC 8 cut(s) 354, 484, 1259, 1355, 1511, 1536, 1616, 1999
HincII GTYRAC 2 cut(s) 1883, 1917
HindII GTYRAC 2 cut(s) 1883, 1917
HindIII AAGCTT 1 cut(s) 146
HphI GGTGA 1 cut(s) 1864
Hpy166II GTNNAC 6 cut(s) 1500, 1723, 1816, 1883, 1917, 1981
Hpy188I TCNGA 8 cut(s) 256, 274, 391, 567, 1113, 1300, 1312, 1438
Hpy188III TCNNGA 7 cut(s) 368, 442, 675, 689, 834, 1273, 1942
Hpy8I GTNNAC 6 cut(s) 1500, 1723, 1816, 1883, 1917, 1981
Hpy99I CGWCG 7 cut(s) 1133, 1136, 1289, 1385, 1388, 1544, 1975
HpyAV CCTTC 7 cut(s) 289, 944, 1202, 1235, 1697, 1727, 1843
HpyCH4III ACNGT 2 cut(s) 1232, 1975
HpyCH4IV ACGT 2 cut(s) 1131, 1748
HpyCH4V TGCA 7 cut(s) 57, 235, 528, 1500, 1546, 1816, 1935
HpyF3I CTNAG 5 cut(s) 21, 253, 770, 1078, 1665
HpySE526I ACGT 2 cut(s) 1131, 1748
Hsp92I GRCGYC 3 cut(s) 309, 1131, 1999
Hsp92II CATG 8 cut(s) 51, 103, 133, 493, 1354, 1431, 1804, 1846
HspAI GCGC 8 cut(s) 354, 484, 1259, 1355, 1511, 1536, 1616, 1999
KasI GGCGCC 1 cut(s) 1998
KpnI GGTACC 1 cut(s) 1596
KroI GCCGGC 1 cut(s) 1255
KroNI GCCGGC 1 cut(s) 1257
Kzo9I GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1854
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 9 cut(s) 38, 166, 275, 881, 1177, 1363, 1444, 1578, 1678
Lsp1109I GCAGC 6 cut(s) 29, 338, 1072, 1344, 1576, 1767
LweI GCATC 5 cut(s) 537, 1254, 1303, 1314, 1316
MaeI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
MaeII ACGT 2 cut(s) 1131, 1748
MaeIII GTNAC 2 cut(s) 66, 1577
MalI GATC 8 cut(s) 564, 838, 1086, 1252, 1304, 1457, 1586, 1856
MbiI CCGCTC 3 cut(s) 1447, 1522, 1828
MboI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1854
MflI RGATCY 1 cut(s) 1455
MhlI GDGCHC 2 cut(s) 1502, 1818
MluCI AATT 6 cut(s) 222, 296, 593, 1203, 1277, 1318
Mly113I GGCGCC 1 cut(s) 1999
MlyI GAGTC 5 cut(s) 464, 527, 835, 1466, 1619
MmeI TCCRAC 3 cut(s) 986, 1155, 1767
MreI CGCCGGCG 1 cut(s) 1255
MroNI GCCGGC 1 cut(s) 1255
MseI TTAA 3 cut(s) 719, 1010, 1280
MslI CAYNNNNRTG 2 cut(s) 83, 523
MspA1I CMGCKG 2 cut(s) 20, 351
MspCI CTTAAG 1 cut(s) 718
MspR9I CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1921
MvaI CCWGG 1 cut(s) 776
MvnI CGCG 6 cut(s) 486, 1511, 1536, 1583, 1616, 1727
NaeI GCCGGC 1 cut(s) 1257
NarI GGCGCC 1 cut(s) 1999
NciI CCSGG 5 cut(s) 263, 264, 825, 1606, 1921
NcoI CCATGG 2 cut(s) 1350, 1427
NdeII GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1854
NgoMIV GCCGGC 1 cut(s) 1255
NheI GCTAGC 1 cut(s) 1073
NlaIII CATG 8 cut(s) 51, 103, 133, 493, 1354, 1431, 1804, 1846
NlaIV GGNNCC 6 cut(s) 271, 342, 422, 1440, 1594, 2000
NmuCI GTSAC 1 cut(s) 1577
NspI RCATGY 1 cut(s) 103
NspV TTCGAA 1 cut(s) 282
OliI CACNNNNGTG 1 cut(s) 523
PaeR7I CTCGAG 2 cut(s) 1064, 1490
PciI ACATGT 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 3 cut(s) 582, 1137, 1967
PdiI GCCGGC 1 cut(s) 1257
PfeI GAWTC 7 cut(s) 628, 692, 828, 1150, 1335, 1771, 1958
PflFI GACNNNGTC 1 cut(s) 710
PfoI TCCNGGA 1 cut(s) 823
Ple19I CGATCG 1 cut(s) 1587
PleI GAGTC 5 cut(s) 463, 527, 835, 1466, 1619
PluTI GGCGCC 1 cut(s) 2002
PmaCI CACGTG 1 cut(s) 1749
PmlI CACGTG 1 cut(s) 1749
PpsI GAGTC 5 cut(s) 463, 527, 835, 1466, 1619
Ppu21I YACGTR 1 cut(s) 1749
PpuMI RGGWCCY 1 cut(s) 341
PscI ACATGT 1 cut(s) 99
PshAI GACNNNNGTC 1 cut(s) 1629
Psp5II RGGWCCY 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 774
PspCI CACGTG 1 cut(s) 1749
PspEI GGTNACC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 774
PspN4I GGNNCC 6 cut(s) 271, 342, 422, 1440, 1594, 2000
PspPI GGNCC 5 cut(s) 341, 582, 712, 1635, 1896
PspPPI RGGWCCY 1 cut(s) 341
PspXI VCTCGAGB 2 cut(s) 1064, 1490
PstNI CAGNNNCTG 2 cut(s) 1483, 1780
PsuI RGATCY 1 cut(s) 1455
PsyI GACNNNGTC 1 cut(s) 710
PvuI CGATCG 1 cut(s) 1587
PvuII CAGCTG 1 cut(s) 20
RgaI GCGATCGC 1 cut(s) 1587
RsaI GTAC 4 cut(s) 386, 803, 1594, 1722
RsaNI GTAC 4 cut(s) 385, 802, 1593, 1721
RseI CAYNNNNRTG 2 cut(s) 83, 523
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 3 cut(s) 719, 1010, 1280
Sau3AI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1854
Sau96I GGNCC 5 cut(s) 341, 582, 712, 1635, 1896
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 5 cut(s) 464, 527, 835, 1466, 1619
ScrFI CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1921
SduI GDGCHC 2 cut(s) 1502, 1818
SfaAI GCGATCGC 1 cut(s) 1587
SfaNI GCATC 5 cut(s) 537, 1254, 1303, 1314, 1316
SfoI GGCGCC 1 cut(s) 2000
Sfr274I CTCGAG 2 cut(s) 1064, 1490
SfuI TTCGAA 1 cut(s) 282
SgfI GCGATCGC 1 cut(s) 1587
SgrAI CRCCGGYG 1 cut(s) 1255
SinI GGWCC 2 cut(s) 341, 712
SlaI CTCGAG 2 cut(s) 1064, 1490
SmaI CCCGGG 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 83, 523
SmlI CTYRAG 6 cut(s) 429, 718, 832, 1064, 1490, 1823
SmoI CTYRAG 6 cut(s) 429, 718, 832, 1064, 1490, 1823
Sse9I AATT 6 cut(s) 222, 296, 593, 1203, 1277, 1318
SspDI GGCGCC 1 cut(s) 1998
SspMI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
StyD4I CCNGG 6 cut(s) 261, 262, 774, 823, 1604, 1919
StyI CCWWGG 3 cut(s) 508, 1350, 1427
TaaI ACNGT 2 cut(s) 1232, 1975
TaiI ACGT 2 cut(s) 1134, 1751
TasI AATT 6 cut(s) 222, 296, 593, 1203, 1277, 1318
TatI WGTACW 2 cut(s) 384, 801
TauI GCSGC 8 cut(s) 270, 293, 1037, 1511, 1531, 1621, 1746, 1999
TfiI GAWTC 7 cut(s) 628, 692, 828, 1150, 1335, 1771, 1958
Tru1I TTAA 3 cut(s) 719, 1010, 1280
Tru9I TTAA 3 cut(s) 719, 1010, 1280
TscAI CASTG 3 cut(s) 67, 242, 501
TseFI GTSAC 1 cut(s) 1577
TseI GCWGC 6 cut(s) 17, 351, 1060, 1357, 1564, 1780
Tsp45I GTSAC 1 cut(s) 1577
TspDTI ATGAA 6 cut(s) 98, 603, 606, 770, 1233, 1859
TspGWI ACGGA 3 cut(s) 179, 777, 1565
TspMI CCCGGG 1 cut(s) 262
TspRI CASTG 3 cut(s) 67, 242, 501
Tth111I GACNNNGTC 1 cut(s) 710
Vha464I CTTAAG 1 cut(s) 718
VneI GTGCAC 2 cut(s) 1498, 1814
VpaK11BI GGWCC 2 cut(s) 341, 712
XapI RAATTY 3 cut(s) 296, 593, 1318
XbaI TCTAGA 2 cut(s) 367, 674
XceI RCATGY 1 cut(s) 103
XhoI CTCGAG 2 cut(s) 1064, 1490
XmaI CCCGGG 1 cut(s) 262
XmaJI CCTAGG 1 cut(s) 508
XspI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
ZraI GACGTC 1 cut(s) 1132
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.