Rw2G012650

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006338
Physical Location & Seq
Reverse (-)
13207251 .. 13210271
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012650.1

Sequence Viewer

Length: 2001 bp
ATGGAGATAACAGAAGCAGCTGAGAAACCAAGCTCCAAAGGCCAAGCATGGTTCTGCACCACTGGGTTACCAAGCGACATTGTGGTGGAAGTTGAAGACATGTCCTTTCATCTTCACAAGTTTCCTCTCATGTCGAAAAGCCGAAAGCTTCACAACCTAATCACAGAGCAAGAGGCTAACCCATCACATCCCCAACAAGAGCTAGAAGAAGAGCCGGACGAAATTGAAGAAGTGCAGTGTCAAATAGTCCTCTCAGACTTCCCGGGCGGCTCCGAGACCTTCGAAATCGCCGCCAAATTCTGCTACGGCGTCAAAATCGACCTCAACTCGACCAATGTGGGACCTCTCCGCTGCGCCGGAGAGTATCTAGAAATGACAGAAGAGTACTCCGAAGACAACCTCATCTCAAAAACCGAAAGGTTCCTGTCTCAAGCCGTGTTCAAGAGCTTCAAAGAGTCCATCAAAGCTCTCAAATCCTGCGAGCGCGTCATGCCACTCGCAGAGACCTTAGGCATTACACAGAGTTGCATCGACTCCATCGCTAACAGAGCCACCAACGCCGATCCTAGCCTGTTCGGCTGGCCGGTCAATGAAGTCGCCCCGAACCCAAGTTCATCCAAACACCAGATTCTGTGGAATGGGATTGACACAGGTGGAAGAAGAAAAGGGATGTCTAGAGCAAACCATCCAGATTCTTGGCTCGAAGACTTGGTCCTCTTAAGCCTCCCTTTCTTCAAGCGATTGATTATGGCAATGAAAGACGGAAATCTCAGCCTGGAAATGGTGGAGACCTGTTTGATGTACTACGCCAAGAAATACATTCCCGGAATCTCAAGATCGACTCGAAAGTCCTCTTCTTCCACCACCACTTCTAGCTCCTCTTTAGCTTTGGAAAACCAACAGAGAGAACTATTGGAGACCATAATCTCAAACCTTCCATTGGAGAAATCACCAAGACCTCCAACCAATACAAGATTTCTATTTGGGCTGTTGAGAACAGCGAATATCTTAAACGCCTCGCAGGGATGTAAAGCCGCATTGGAGAAGAAGATTGGGTCGCAGCTCGAGCAAGCTAGCTTAGACGATCTGCTCATACCGAGCTATTCGTATCTGAATGAGACCCTATACGACGTCGATTGCGTGGAGAGGATTCTGGGGTATTTTTTGGACGGGTTGGAGCAGAGAAACAGAAACAGAGATGGAATTGAAGGTGGAGATGAAGACGAAGACCGTGATATAGAAGGTGCTAGATCGCCGGCGCTGATGCTTGTCGGGAAATTAACCGACGAGTTTCTCTCCGAGATCGCATCGGATGCGAATTTGATGCCGGATAGATTCTACAACTTTGCCATGGCCCTGCCGGAGCAGGCGAGGCTGTTCGACGACGGGCTTTACAGAGCAGTTGATGTTTATCTAAAGGCTCATCCATGGATATCGGAACCGGAGCGGGAGAAGATCTGCGGGATACTAGACTCCCAGAAGCTCACACTGGAGGCGTGCACGCACGCCGCGCAGAACGAGCGGTTGCCGCTACGCGCCGTCGTGCAAGTCTTGTTCTTCGAGCAGCTTCAGCTCCGTCACGCGATCGCCGGTACCCTTATCGCCGGGGAGACCGCGCCGCCGGACTCCAGTCGGCCCTCGGTGTTGCGAAGAGAGGCGGAGACTGAGGAGGAGGAGGAGGAGGAAGGCGGTGCTGTGGAGGTACACGCGGAGGAAGGAGGAGGCGGCACGTGGAGGTCGGCTGTGAGGGAGAATCAGGTGCTGCGGTTGGATATGGATAGCATGAGGACGCGGGTGCACCAACTTGAGCGGGAGTGTTCCAACATGAAGAAGGTGATCGAAAAGATTGACAACAAATCGGGTCAACCCGATGGACGGGCCGGATGGAAAGGCTCGTTGACCCGGCGGTTCGGGTGCAAGTTCAAGACCCAAGTGTGCGATTCGCACGAGCCGACGGTCGTGGACGGGCGGAAGGGGCGGCGCCACCAGCATCAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

666

Amino Acids

74.72

Weight (kDa)

5.13

Isoelectric Point (pI)

56.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 231 - 500 4.2e-85 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 847
AatII GACGTC 1 cut(s) 1134
Acc65I GGTACC 1 cut(s) 1592
AccB1I GGYRCC 2 cut(s) 1592, 1980
AccBSI CCGCTC 3 cut(s) 1447, 1522, 1810
AccII CGCG 7 cut(s) 486, 1511, 1536, 1583, 1616, 1709, 1792
AclWI GGATC 1 cut(s) 557
AcoI YGGCCR 1 cut(s) 581
AcsI RAATTY 2 cut(s) 296, 1318
AcuI CTGAAG 1 cut(s) 1553
AcvI CACGTG 1 cut(s) 1731
AcyI GRCGYC 3 cut(s) 309, 1131, 1981
AfaI GTAC 4 cut(s) 386, 803, 1594, 1704
AfiI CCNNNNNNNGG 3 cut(s) 781, 940, 1875
AflII CTTAAG 1 cut(s) 718
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 7 cut(s) 95, 227, 442, 451, 736, 1208, 1924
AjnI CCWGG 1 cut(s) 774
Alw21I GWGCWC 2 cut(s) 1502, 1800
Alw26I GTCTC 8 cut(s) 269, 432, 497, 782, 911, 1112, 1604, 1655
Alw44I GTGCAC 2 cut(s) 1498, 1796
AlwI GGATC 1 cut(s) 557
AlwNI CAGNNNCTG 2 cut(s) 631, 1762
Ama87I CYCGRG 2 cut(s) 262, 1064
AoxI GGCC 5 cut(s) 40, 581, 1353, 1634, 1878
ApaLI GTGCAC 2 cut(s) 1498, 1796
ApeKI GCWGC 5 cut(s) 17, 351, 1060, 1564, 1762
ApoI RAATTY 2 cut(s) 296, 1318
ArsI GACNNNNNNTTYG 2 cut(s) 696, 728
AsiSI GCGATCGC 1 cut(s) 1587
Asp718I GGTACC 1 cut(s) 1592
AspLEI GCGC 7 cut(s) 356, 486, 1261, 1513, 1538, 1618, 1983
AspS9I GGNCC 5 cut(s) 341, 712, 1354, 1635, 1878
AsuC2I CCSGG 5 cut(s) 263, 264, 825, 1606, 1903
AsuHPI GGTGA 2 cut(s) 942, 1846
AsuII TTCGAA 1 cut(s) 282
AsuNHI GCTAGC 1 cut(s) 1073
AvaI CYCGRG 2 cut(s) 262, 1064
AvaII GGWCC 2 cut(s) 341, 712
AxyI CCTNAGG 1 cut(s) 508
BaeGI GKGCMC 2 cut(s) 1502, 1800
BanI GGYRCC 2 cut(s) 1592, 1980
BauI CACGAG 1 cut(s) 1946
BbrPI CACGTG 1 cut(s) 1731
BbsI GAAGAC 5 cut(s) 102, 399, 711, 1227, 1233
Bbv12I GWGCWC 2 cut(s) 1502, 1800
BbvI GCAGC 5 cut(s) 29, 338, 1072, 1576, 1749
BccI CCATC 7 cut(s) 190, 467, 545, 693, 1193, 1865, 1878
BceAI ACGGC 3 cut(s) 322, 419, 1523
BcgI CGANNNNNNTGC 2 cut(s) 1306, 1340
BciT130I CCWGG 1 cut(s) 776
BciVI GTATCC 1 cut(s) 1458
BcnI CCSGG 5 cut(s) 263, 264, 825, 1606, 1903
BcoDI GTCTC 8 cut(s) 269, 432, 497, 782, 911, 1112, 1604, 1655
BfaI CTAG 8 cut(s) 203, 368, 567, 675, 873, 1074, 1248, 1469
BfoI RGCGCY 2 cut(s) 1262, 1984
BfrI CTTAAG 1 cut(s) 718
BfuI GTATCC 1 cut(s) 1458
BglI GCCNNNNNGGC 1 cut(s) 576
BglII AGATCT 1 cut(s) 1455
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1903
Bme18I GGWCC 2 cut(s) 341, 712
BmeT110I CYCGRG 2 cut(s) 262, 1064
BmgT120I GGNCC 5 cut(s) 341, 712, 1354, 1635, 1878
BmiI GGNNCC 6 cut(s) 271, 342, 422, 1440, 1594, 1982
BmrFI CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1903
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 5 cut(s) 537, 1254, 1303, 1314, 1316
BmtI GCTAGC 1 cut(s) 1077
BmuI ACTGGG 1 cut(s) 72
BoxI GACNNNNGTC 1 cut(s) 1629
BpiI GAAGAC 5 cut(s) 102, 399, 711, 1227, 1233
BplI GAGNNNNNCTC 2 cut(s) 1280, 1312
BpmI CTGGAG 2 cut(s) 1511, 1612
Bpu14I TTCGAA 1 cut(s) 282
BpuEI CTTGAG 3 cut(s) 414, 817, 1826
BpuMI CCSGG 5 cut(s) 263, 264, 825, 1606, 1903
BsaAI YACGTR 1 cut(s) 1731
BsaBI GATNNNNATC 1 cut(s) 1410
BsaHI GRCGYC 3 cut(s) 309, 1131, 1981
BsaI GGTCTC 6 cut(s) 269, 497, 782, 911, 1112, 1604
BsaJI CCNNGG 5 cut(s) 262, 1350, 1427, 1605, 1638
BsaWI WCCGGW 1 cut(s) 1441
Bsc4I CCNNNNNNNGG 3 cut(s) 781, 940, 1875
Bse118I RCCGGY 3 cut(s) 583, 1255, 1589
Bse1I ACTGG 3 cut(s) 67, 1494, 1629
Bse21I CCTNAGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 759
Bse8I GATNNNNATC 1 cut(s) 1410
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 5 cut(s) 262, 1350, 1427, 1605, 1638
BseGI GGATG 8 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1889
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 3 cut(s) 781, 940, 1875
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 4 cut(s) 12, 267, 784, 1656
BseNI ACTGG 3 cut(s) 67, 1494, 1629
BseRI GAGGAG 7 cut(s) 868, 1682, 1685, 1688, 1691, 1694, 1734
BseSI GKGCMC 2 cut(s) 1502, 1800
BseXI GCAGC 5 cut(s) 29, 338, 1072, 1576, 1749
BsgI GTGCAG 2 cut(s) 40, 254
Bsh1236I CGCG 7 cut(s) 486, 1511, 1536, 1583, 1616, 1709, 1792
Bsh1285I CGRYCG 2 cut(s) 1587, 1959
BshFI GGCC 5 cut(s) 42, 583, 1355, 1636, 1880
BshNI GGYRCC 2 cut(s) 1592, 1980
BsiEI CGRYCG 2 cut(s) 1587, 1959
BsiHKAI GWGCWC 2 cut(s) 1502, 1800
BsiHKCI CYCGRG 2 cut(s) 262, 1064
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 3 cut(s) 781, 940, 1875
BsmAI GTCTC 8 cut(s) 269, 432, 497, 782, 911, 1112, 1604, 1655
BsmFI GGGAC 1 cut(s) 354
BsnI GGCC 5 cut(s) 42, 583, 1355, 1636, 1880
Bso31I GGTCTC 6 cut(s) 269, 497, 782, 911, 1112, 1604
BsoBI CYCGRG 2 cut(s) 262, 1064
Bsp119I TTCGAA 1 cut(s) 282
Bsp1286I GDGCHC 2 cut(s) 1502, 1800
Bsp143I GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1836
Bsp19I CCATGG 2 cut(s) 1350, 1427
BspANI GGCC 5 cut(s) 42, 583, 1355, 1636, 1880
BspCNI CTCAG 4 cut(s) 13, 266, 783, 1657
BspFNI CGCG 7 cut(s) 486, 1511, 1536, 1583, 1616, 1709, 1792
BspLI GGNNCC 6 cut(s) 271, 342, 422, 1440, 1594, 1982
BspOI GCTAGC 1 cut(s) 1077
BspPI GGATC 1 cut(s) 557
BspQI GCTCTTC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 282
BspT107I GGYRCC 2 cut(s) 1592, 1980
BspTI CTTAAG 1 cut(s) 718
BspTNI GGTCTC 6 cut(s) 269, 497, 782, 911, 1112, 1604
BsrBI CCGCTC 3 cut(s) 1447, 1522, 1810
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 3 cut(s) 583, 1255, 1589
BsrI ACTGG 3 cut(s) 67, 1494, 1629
BssAI RCCGGY 3 cut(s) 583, 1255, 1589
BssECI CCNNGG 5 cut(s) 262, 1350, 1427, 1605, 1638
BssMI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1836
BssNI GRCGYC 3 cut(s) 309, 1131, 1981
BssSI CACGAG 1 cut(s) 1946
BssT1I CCWWGG 2 cut(s) 1350, 1427
Bst2BI CACGAG 1 cut(s) 1946
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 2 cut(s) 1232, 1957
Bst6I CTCTTC 4 cut(s) 204, 375, 859, 1645
BstACI GRCGYC 3 cut(s) 309, 1131, 1981
BstAFI CTTAAG 1 cut(s) 718
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBAI YACGTR 1 cut(s) 1731
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 9 cut(s) 482, 581, 1071, 1075, 1257, 1368, 1498, 1502, 1506
BstDEI CTNAG 6 cut(s) 21, 253, 508, 770, 1078, 1665
BstDSI CCRYGG 2 cut(s) 1350, 1427
BstEII GGTNACC 1 cut(s) 66
BstF5I GGATG 8 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1889
BstFNI CGCG 7 cut(s) 486, 1511, 1536, 1583, 1616, 1709, 1792
BstH2I RGCGCY 2 cut(s) 1262, 1984
BstHHI GCGC 7 cut(s) 356, 486, 1261, 1513, 1538, 1618, 1983
BstKTI GATC 8 cut(s) 565, 839, 1087, 1253, 1305, 1458, 1587, 1839
BstMAI GTCTC 8 cut(s) 269, 432, 497, 782, 911, 1112, 1604, 1655
BstMBI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1836
BstMCI CGRYCG 2 cut(s) 1587, 1959
BstNI CCWGG 1 cut(s) 776
BstNSI RCATGY 1 cut(s) 103
BstPAI GACNNNNGTC 1 cut(s) 1629
BstPI GGTNACC 1 cut(s) 66
BstSCI CCNGG 6 cut(s) 261, 262, 774, 823, 1604, 1901
BstSLI GKGCMC 2 cut(s) 1502, 1800
BstUI CGCG 7 cut(s) 486, 1511, 1536, 1583, 1616, 1709, 1792
BstV1I GCAGC 5 cut(s) 29, 338, 1072, 1576, 1749
BstV2I GAAGAC 5 cut(s) 102, 399, 711, 1227, 1233
BstX2I RGATCY 1 cut(s) 1455
BstXI CCANNNNNNTGG 1 cut(s) 696
BstYI RGATCY 1 cut(s) 1455
Bsu36I CCTNAGG 1 cut(s) 508
BsuI GTATCC 1 cut(s) 1458
BsuRI GGCC 5 cut(s) 42, 583, 1355, 1636, 1880
BtgI CCRYGG 2 cut(s) 1350, 1427
BtgZI GCGATG 1 cut(s) 523
BtsCI GGATG 8 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1889
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 3 cut(s) 60, 242, 1487
Cac8I GCNNGC 9 cut(s) 482, 581, 1071, 1075, 1257, 1368, 1498, 1502, 1506
CaiI CAGNNNCTG 2 cut(s) 631, 1762
CfoI GCGC 7 cut(s) 356, 486, 1261, 1513, 1538, 1618, 1983
Cfr10I RCCGGY 3 cut(s) 583, 1255, 1589
Cfr13I GGNCC 5 cut(s) 341, 712, 1354, 1635, 1878
Cfr9I CCCGGG 1 cut(s) 262
CseI GACGC 3 cut(s) 298, 475, 1798
Csp6I GTAC 4 cut(s) 385, 802, 1593, 1703
CviAII CATG 8 cut(s) 48, 100, 130, 490, 1351, 1428, 1783, 1825
CviQI GTAC 4 cut(s) 385, 802, 1593, 1703
DdeI CTNAG 6 cut(s) 21, 253, 508, 770, 1078, 1665
DinI GGCGCC 1 cut(s) 1982
DpnI GATC 8 cut(s) 564, 838, 1086, 1252, 1304, 1457, 1586, 1838
DpnII GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1836
DrdI GACNNNNNNGTC 1 cut(s) 847
DseDI GACNNNNNNGTC 1 cut(s) 847
EaeI YGGCCR 1 cut(s) 581
Eam1104I CTCTTC 4 cut(s) 204, 375, 859, 1645
EarI CTCTTC 4 cut(s) 204, 375, 859, 1645
EciI GGCGGA 2 cut(s) 1673, 1984
Eco130I CCWWGG 2 cut(s) 1350, 1427
Eco31I GGTCTC 6 cut(s) 269, 497, 782, 911, 1112, 1604
Eco32I GATATC 1 cut(s) 1434
Eco47I GGWCC 2 cut(s) 341, 712
Eco57I CTGAAG 1 cut(s) 1553
Eco72I CACGTG 1 cut(s) 1731
Eco81I CCTNAGG 1 cut(s) 508
Eco88I CYCGRG 2 cut(s) 262, 1064
Eco91I GGTNACC 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 774
EcoRV GATATC 1 cut(s) 1434
EcoT14I CCWWGG 2 cut(s) 1350, 1427
EgeI GGCGCC 1 cut(s) 1982
EheI GGCGCC 1 cut(s) 1982
ErhI CCWWGG 2 cut(s) 1350, 1427
FaeI CATG 8 cut(s) 51, 103, 133, 493, 1354, 1431, 1786, 1828
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FaqI GGGAC 1 cut(s) 354
FatI CATG 8 cut(s) 47, 99, 129, 489, 1350, 1427, 1782, 1824
FauI CCCGC 4 cut(s) 1440, 1454, 1785, 1803
FokI GGATG 8 cut(s) 174, 601, 672, 682, 1038, 1325, 1410, 1896
FspBI CTAG 8 cut(s) 203, 368, 567, 675, 873, 1074, 1248, 1469
GlaI GCGC 7 cut(s) 355, 485, 1260, 1512, 1537, 1617, 1982
GsuI CTGGAG 2 cut(s) 1511, 1612
HaeII RGCGCY 2 cut(s) 1262, 1984
HaeIII GGCC 5 cut(s) 42, 583, 1355, 1636, 1880
HgaI GACGC 3 cut(s) 298, 475, 1798
HhaI GCGC 7 cut(s) 356, 486, 1261, 1513, 1538, 1618, 1983
Hin1I GRCGYC 3 cut(s) 309, 1131, 1981
Hin1II CATG 8 cut(s) 51, 103, 133, 493, 1354, 1431, 1786, 1828
Hin6I GCGC 7 cut(s) 354, 484, 1259, 1511, 1536, 1616, 1981
HinP1I GCGC 7 cut(s) 354, 484, 1259, 1511, 1536, 1616, 1981
HincII GTYRAC 2 cut(s) 1865, 1899
HindII GTYRAC 2 cut(s) 1865, 1899
HindIII AAGCTT 1 cut(s) 146
HphI GGTGA 2 cut(s) 942, 1846
Hpy166II GTNNAC 6 cut(s) 1500, 1705, 1798, 1865, 1899, 1963
Hpy188I TCNGA 7 cut(s) 256, 274, 391, 1113, 1300, 1312, 1438
Hpy188III TCNNGA 7 cut(s) 368, 442, 675, 689, 834, 1273, 1924
Hpy8I GTNNAC 6 cut(s) 1500, 1705, 1798, 1865, 1899, 1963
Hpy99I CGWCG 7 cut(s) 1133, 1136, 1289, 1385, 1388, 1544, 1957
HpyAV CCTTC 8 cut(s) 289, 944, 1202, 1235, 1679, 1709, 1825, 1966
HpyCH4III ACNGT 2 cut(s) 1232, 1957
HpyCH4IV ACGT 2 cut(s) 1131, 1730
HpyCH4V TGCA 7 cut(s) 57, 235, 528, 1500, 1546, 1798, 1917
HpyF3I CTNAG 6 cut(s) 21, 253, 508, 770, 1078, 1665
HpySE526I ACGT 2 cut(s) 1131, 1730
Hsp92I GRCGYC 3 cut(s) 309, 1131, 1981
Hsp92II CATG 8 cut(s) 51, 103, 133, 493, 1354, 1431, 1786, 1828
HspAI GCGC 7 cut(s) 354, 484, 1259, 1511, 1536, 1616, 1981
KasI GGCGCC 1 cut(s) 1980
KpnI GGTACC 1 cut(s) 1596
KroI GCCGGC 1 cut(s) 1255
KroNI GCCGGC 1 cut(s) 1257
Kzo9I GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1836
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 7 cut(s) 38, 275, 881, 1177, 1363, 1444, 1578
Lsp1109I GCAGC 5 cut(s) 29, 338, 1072, 1576, 1749
LweI GCATC 5 cut(s) 537, 1254, 1303, 1314, 1316
MaeI CTAG 8 cut(s) 203, 368, 567, 675, 873, 1074, 1248, 1469
MaeII ACGT 2 cut(s) 1131, 1730
MaeIII GTNAC 2 cut(s) 66, 1577
MalI GATC 8 cut(s) 564, 838, 1086, 1252, 1304, 1457, 1586, 1838
MbiI CCGCTC 3 cut(s) 1447, 1522, 1810
MboI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1836
MflI RGATCY 1 cut(s) 1455
MhlI GDGCHC 2 cut(s) 1502, 1800
MluCI AATT 5 cut(s) 222, 296, 1203, 1277, 1318
Mly113I GGCGCC 1 cut(s) 1981
MlyI GAGTC 5 cut(s) 464, 527, 835, 1466, 1619
MmeI TCCRAC 4 cut(s) 986, 1155, 1749, 1845
MreI CGCCGGCG 1 cut(s) 1255
MroNI GCCGGC 1 cut(s) 1255
MseI TTAA 3 cut(s) 719, 1010, 1280
MslI CAYNNNNRTG 1 cut(s) 83
MspA1I CMGCKG 2 cut(s) 20, 351
MspCI CTTAAG 1 cut(s) 718
MspR9I CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1903
MvaI CCWGG 1 cut(s) 776
MvnI CGCG 7 cut(s) 486, 1511, 1536, 1583, 1616, 1709, 1792
NaeI GCCGGC 1 cut(s) 1257
NarI GGCGCC 1 cut(s) 1981
NciI CCSGG 5 cut(s) 263, 264, 825, 1606, 1903
NcoI CCATGG 2 cut(s) 1350, 1427
NdeII GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1836
NgoMIV GCCGGC 1 cut(s) 1255
NheI GCTAGC 1 cut(s) 1073
NlaIII CATG 8 cut(s) 51, 103, 133, 493, 1354, 1431, 1786, 1828
NlaIV GGNNCC 6 cut(s) 271, 342, 422, 1440, 1594, 1982
NmuCI GTSAC 1 cut(s) 1577
NspI RCATGY 1 cut(s) 103
NspV TTCGAA 1 cut(s) 282
PaeR7I CTCGAG 1 cut(s) 1064
PciI ACATGT 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 2 cut(s) 1137, 1949
PdiI GCCGGC 1 cut(s) 1257
PfeI GAWTC 7 cut(s) 628, 692, 828, 1150, 1335, 1753, 1940
PflFI GACNNNGTC 1 cut(s) 710
PfoI TCCNGGA 1 cut(s) 823
Ple19I CGATCG 1 cut(s) 1587
PleI GAGTC 5 cut(s) 463, 527, 835, 1466, 1619
PluTI GGCGCC 1 cut(s) 1984
PmaCI CACGTG 1 cut(s) 1731
PmlI CACGTG 1 cut(s) 1731
PpsI GAGTC 5 cut(s) 463, 527, 835, 1466, 1619
Ppu21I YACGTR 1 cut(s) 1731
PpuMI RGGWCCY 1 cut(s) 341
PscI ACATGT 1 cut(s) 99
PshAI GACNNNNGTC 1 cut(s) 1629
Psp5II RGGWCCY 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 774
PspCI CACGTG 1 cut(s) 1731
PspEI GGTNACC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 774
PspN4I GGNNCC 6 cut(s) 271, 342, 422, 1440, 1594, 1982
PspPI GGNCC 5 cut(s) 341, 712, 1354, 1635, 1878
PspPPI RGGWCCY 1 cut(s) 341
PspXI VCTCGAGB 1 cut(s) 1064
PstNI CAGNNNCTG 2 cut(s) 631, 1762
PsuI RGATCY 1 cut(s) 1455
PsyI GACNNNGTC 1 cut(s) 710
PvuI CGATCG 1 cut(s) 1587
PvuII CAGCTG 1 cut(s) 20
RgaI GCGATCGC 1 cut(s) 1587
RsaI GTAC 4 cut(s) 386, 803, 1594, 1704
RsaNI GTAC 4 cut(s) 385, 802, 1593, 1703
RseI CAYNNNNRTG 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 3 cut(s) 719, 1010, 1280
Sau3AI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1836
Sau96I GGNCC 5 cut(s) 341, 712, 1354, 1635, 1878
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 5 cut(s) 464, 527, 835, 1466, 1619
ScrFI CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1903
SduI GDGCHC 2 cut(s) 1502, 1800
SfaAI GCGATCGC 1 cut(s) 1587
SfaNI GCATC 5 cut(s) 537, 1254, 1303, 1314, 1316
SfoI GGCGCC 1 cut(s) 1982
Sfr274I CTCGAG 1 cut(s) 1064
SfuI TTCGAA 1 cut(s) 282
SgfI GCGATCGC 1 cut(s) 1587
SgrAI CRCCGGYG 1 cut(s) 1255
SinI GGWCC 2 cut(s) 341, 712
SlaI CTCGAG 1 cut(s) 1064
SmaI CCCGGG 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 5 cut(s) 429, 718, 832, 1064, 1805
SmoI CTYRAG 5 cut(s) 429, 718, 832, 1064, 1805
Sse9I AATT 5 cut(s) 222, 296, 1203, 1277, 1318
SspDI GGCGCC 1 cut(s) 1980
SspMI CTAG 8 cut(s) 203, 368, 567, 675, 873, 1074, 1248, 1469
StyD4I CCNGG 6 cut(s) 261, 262, 774, 823, 1604, 1901
StyI CCWWGG 2 cut(s) 1350, 1427
TaaI ACNGT 2 cut(s) 1232, 1957
TaiI ACGT 2 cut(s) 1134, 1733
TasI AATT 5 cut(s) 222, 296, 1203, 1277, 1318
TatI WGTACW 2 cut(s) 384, 801
TauI GCSGC 8 cut(s) 270, 293, 1037, 1511, 1531, 1621, 1728, 1981
TfiI GAWTC 7 cut(s) 628, 692, 828, 1150, 1335, 1753, 1940
Tru1I TTAA 3 cut(s) 719, 1010, 1280
Tru9I TTAA 3 cut(s) 719, 1010, 1280
TscAI CASTG 3 cut(s) 67, 242, 1494
TseFI GTSAC 1 cut(s) 1577
TseI GCWGC 5 cut(s) 17, 351, 1060, 1564, 1762
Tsp45I GTSAC 1 cut(s) 1577
TspDTI ATGAA 6 cut(s) 98, 603, 606, 770, 1233, 1841
TspGWI ACGGA 2 cut(s) 777, 1565
TspMI CCCGGG 1 cut(s) 262
TspRI CASTG 3 cut(s) 67, 242, 1494
Tth111I GACNNNGTC 1 cut(s) 710
Vha464I CTTAAG 1 cut(s) 718
VneI GTGCAC 2 cut(s) 1498, 1796
VpaK11BI GGWCC 2 cut(s) 341, 712
XapI RAATTY 2 cut(s) 296, 1318
XbaI TCTAGA 2 cut(s) 367, 674
XceI RCATGY 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 1064
XmaI CCCGGG 1 cut(s) 262
XspI CTAG 8 cut(s) 203, 368, 567, 675, 873, 1074, 1248, 1469
ZraI GACGTC 1 cut(s) 1132
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.