Rroxscaffold_2G00139850

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006338
Physical Location & Seq
Forward (+)
78149182 .. 78152112
2931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139850.1

Sequence Viewer

Length: 2022 bp
ATGGAGATAACAGAAGCAGCTGAGAAACCAAGCTCCAAAGGCCAAGCATGGTTCTGCACCACTGGGTTACCAAGCGACATTGTGGTGGAAGTTGAAGACATGTCCTTTCATCTTCACAAGTTTCCTCTCATGTCCAAAAGCCGAAAGCTTCACAACCTAATCACAGAGCAAGAGGCCAACCCATCACATCCCCAACAAGAGCTAGAAGAAGAGCCGGACGAAATTGAAGAAGTGCAGTGTCAAATAGTCCTCTCAGACTTCCCGGGCGGCTCCGAGACCTTCGAAATCGCCGCCAAATTCTGCTACGGCGTCAAAATCGACCTCAACTCGACCAATGTGGGACCTCTCCGCTGCGCCGGAGAGTATCTAGAAATGACAGAAGAGTATTCCGAAGACAACCTCATCTCAAAAACAGAAAGGTTCCTCACTCAAGCCGTGTTCAAGAGCTTCAAAGAGTCCATCAAAGCCCTCAAATCCTGCGAGCGCGTCGTGCCACTCGCAGAGACCCTAGGCATTACACAGAGGTGCATCGACTCAATTGCTAACAGAGCCACCAACGCCGATCCGAGCCTGTTCGGCTGGCCCGTCAATGAAGTCGCCCCGAACCCAAGTTCATCCAAACACCAGATTCTATGGAATGGGATTGACACAGGTGGAAGAAGAAAAGGGATGTCTAGAGCAAACCATCCTGATTCTTGGCTCGAAGACTTGGTCCTCTTAAGCCTCCCTTTCTTCAAGCGATTGATTATGGCAATGAAAGACGGAAATCTCAGCCTGGAAATGGTGGAGACCTGTTTGATGTACTACGCCAAGAAATACATTCCCGGAATCTCAAGATCGACTCGAAAGTCCTCTTCTTCCACCACCACTCCTAGCTCCTCTTTAGCTTTGGAAAACCAACAGAGAGAACTATTGGAGACTATAATCTCAAACCTTCCATTGGAGAAAACACCAAGACCTCCAACTAATACAAGATTTCTATTTGGGTTGTTGAGAACAGCGAATATCTTAAACGCCTCGCAGGGATGTAAAGCCGCATTGGAGAAGAAGATTGGTTCGCAGCTCGAGCAAGCTAGCTTAGACGATCTGCTCATACCGAGCTATTCGTATCTCAATGAAACCCTATACGACGTCGATTGCGTGGAGAGGATTCTGGGGTATTTTTTGGACGGGTTGGAGCAGAGAAACAGAAACAGAGATGGAATTGAAGGTGGAGATGAAGATGAAGACCGTGATATAGAAGGTGCTAGATCGCCGGCGCTGATGCTTGTCGGGAAATTAACCGACGAGTATCTCTCCGAGATCGCATCGGATGCGAATTTGAAGCCGGATAGATTCTACAACTTTGCCATGGCGCTGCCGGAGCAGGCGAGGCTGTTCGACGACGGGCTTTACAGAGCAGTTGATGTTTATCTAAAGGCTCATCCATGGATATCGGAACCGGAGCGAGAGAAGATCTGCGGGATACTAGACTCCCAGAAGCTCACACTCGAGGCGTGCACGCACGCCGCGCAGAACGAGCGATTGCCGCTACGCGCCGTCGTGCAAGTCTTGTTCTTCGAGCAGCTTCAGCTCCGTCACGCGATCGCCGGTACCCTTATCGCCGGGGAGACTGCGCCCGCGCCCCCTGACTCCAGTCGGCCCTCGGTGTTGCGAAGAGAGGCAGAGACTGAGGAGGACGAGGAGCAGGAGCAGGAGGAGGAGGAAGGCGGTGCCGTGGAGGTACACGCGGAGGAAGGAGGAGGCGGCACGTGGAGGTCGGCTGTGAGGGAGAATCAGGTGCTGCGGTTGGATATGGATAGCATGAGAACGCGGGTGCATCAACTTGAGCGGGAGTGTTCCAACATGAGGAAGGTGATCGAAAAGATTGACAACAAATCGGGTCAACCGGATGGACGGGCTGGATGGAAAGGCTCGTTGACCCGGCGGTTCGGTTGCAAGTTCAAGACCCAAGTGTGCGATTCGCACGAGCCGACGGTCGTGGACGGGCGGAAAGGGCGGCGCCACCAGCATCAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

673

Amino Acids

75.63

Weight (kDa)

5.11

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 231 - 500 3.2e-86 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 847
AatII GACGTC 1 cut(s) 1134
Acc65I GGTACC 1 cut(s) 1592
AccB1I GGYRCC 3 cut(s) 1592, 1712, 2001
AccBSI CCGCTC 1 cut(s) 1831
AccII CGCG 7 cut(s) 486, 1511, 1536, 1583, 1622, 1730, 1813
AclWI GGATC 1 cut(s) 557
AcsI RAATTY 2 cut(s) 296, 1318
AcuI CTGAAG 1 cut(s) 1553
AcvI CACGTG 1 cut(s) 1752
AcyI GRCGYC 3 cut(s) 309, 1131, 2002
AfaI GTAC 3 cut(s) 803, 1594, 1725
AfiI CCNNNNNNNGG 3 cut(s) 781, 940, 1848
AflII CTTAAG 1 cut(s) 718
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 8 cut(s) 95, 227, 442, 451, 736, 1208, 1324, 1945
AjnI CCWGG 1 cut(s) 774
AleI CACNNNNGTG 1 cut(s) 523
Alw21I GWGCWC 1 cut(s) 1502
Alw26I GTCTC 6 cut(s) 269, 497, 782, 911, 1604, 1661
Alw44I GTGCAC 1 cut(s) 1498
AlwI GGATC 1 cut(s) 557
AlwNI CAGNNNCTG 2 cut(s) 1670, 1783
Ama87I CYCGRG 3 cut(s) 262, 1064, 1490
AoxI GGCC 4 cut(s) 40, 174, 581, 1640
ApaLI GTGCAC 1 cut(s) 1498
ApeKI GCWGC 6 cut(s) 17, 351, 1060, 1357, 1564, 1783
ApoI RAATTY 2 cut(s) 296, 1318
ArsI GACNNNNNNTTYG 2 cut(s) 696, 728
AsiSI GCGATCGC 1 cut(s) 1587
Asp718I GGTACC 1 cut(s) 1592
AspA2I CCTAGG 1 cut(s) 508
AspLEI GCGC 9 cut(s) 356, 486, 1261, 1357, 1513, 1538, 1618, 1624, 2004
AspS9I GGNCC 4 cut(s) 341, 582, 712, 1641
AsuC2I CCSGG 5 cut(s) 263, 264, 825, 1606, 1924
AsuHPI GGTGA 1 cut(s) 1867
AsuII TTCGAA 1 cut(s) 282
AsuNHI GCTAGC 1 cut(s) 1073
AvaI CYCGRG 3 cut(s) 262, 1064, 1490
AvaII GGWCC 2 cut(s) 341, 712
AvrII CCTAGG 1 cut(s) 508
BaeGI GKGCMC 1 cut(s) 1502
BanI GGYRCC 3 cut(s) 1592, 1712, 2001
BauI CACGAG 1 cut(s) 1967
BbrPI CACGTG 1 cut(s) 1752
BbsI GAAGAC 4 cut(s) 102, 399, 711, 1233
Bbv12I GWGCWC 1 cut(s) 1502
BbvI GCAGC 6 cut(s) 29, 338, 1072, 1344, 1576, 1770
BccI CCATC 6 cut(s) 190, 467, 693, 1193, 1886, 1899
BceAI ACGGC 4 cut(s) 322, 419, 1523, 1700
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 1 cut(s) 776
BciVI GTATCC 1 cut(s) 1458
BcnI CCSGG 5 cut(s) 263, 264, 825, 1606, 1924
BcoDI GTCTC 6 cut(s) 269, 497, 782, 911, 1604, 1661
BfaI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
BfoI RGCGCY 3 cut(s) 1262, 1358, 2005
BfrI CTTAAG 1 cut(s) 718
BfuI GTATCC 1 cut(s) 1458
BglI GCCNNNNNGGC 1 cut(s) 576
BglII AGATCT 1 cut(s) 1455
BlnI CCTAGG 1 cut(s) 508
Bme1390I CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1924
Bme18I GGWCC 2 cut(s) 341, 712
BmeT110I CYCGRG 3 cut(s) 262, 1064, 1490
BmgT120I GGNCC 4 cut(s) 341, 582, 712, 1641
BmiI GGNNCC 7 cut(s) 271, 342, 422, 1440, 1594, 1714, 2003
BmrFI CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1924
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 5 cut(s) 537, 1254, 1303, 1316, 1828
BmtI GCTAGC 1 cut(s) 1077
BmuI ACTGGG 1 cut(s) 72
BoxI GACNNNNGTC 1 cut(s) 1635
BpiI GAAGAC 4 cut(s) 102, 399, 711, 1233
BplI GAGNNNNNCTC 2 cut(s) 1280, 1312
BpmI CTGGAG 1 cut(s) 1618
Bpu14I TTCGAA 1 cut(s) 282
BpuEI CTTGAG 3 cut(s) 414, 817, 1847
BpuMI CCSGG 5 cut(s) 263, 264, 825, 1606, 1924
BsaAI YACGTR 1 cut(s) 1752
BsaBI GATNNNNATC 1 cut(s) 1410
BsaHI GRCGYC 3 cut(s) 309, 1131, 2002
BsaI GGTCTC 3 cut(s) 269, 497, 782
BsaJI CCNNGG 7 cut(s) 262, 508, 1350, 1427, 1605, 1644, 1716
BsaWI WCCGGW 2 cut(s) 1441, 1888
BsaXI ACNNNNNCTCC 2 cut(s) 853, 883
Bsc4I CCNNNNNNNGG 3 cut(s) 781, 940, 1848
Bse118I RCCGGY 2 cut(s) 1255, 1589
Bse1I ACTGG 2 cut(s) 67, 1635
Bse3DI GCAATG 1 cut(s) 759
Bse8I GATNNNNATC 1 cut(s) 1410
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 7 cut(s) 262, 508, 1350, 1427, 1605, 1644, 1716
BseGI GGATG 9 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1897, 1910
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 3 cut(s) 781, 940, 1848
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 4 cut(s) 12, 267, 784, 1662
BseNI ACTGG 2 cut(s) 67, 1635
BseRI GAGGAG 6 cut(s) 868, 1688, 1697, 1712, 1715, 1755
BseSI GKGCMC 1 cut(s) 1502
BseXI GCAGC 6 cut(s) 29, 338, 1072, 1344, 1576, 1770
BsgI GTGCAG 2 cut(s) 40, 254
Bsh1236I CGCG 7 cut(s) 486, 1511, 1536, 1583, 1622, 1730, 1813
Bsh1285I CGRYCG 2 cut(s) 1587, 1980
BshFI GGCC 4 cut(s) 42, 176, 583, 1642
BshNI GGYRCC 3 cut(s) 1592, 1712, 2001
BsiEI CGRYCG 2 cut(s) 1587, 1980
BsiHKAI GWGCWC 1 cut(s) 1502
BsiHKCI CYCGRG 3 cut(s) 262, 1064, 1490
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 3 cut(s) 781, 940, 1848
BsmAI GTCTC 6 cut(s) 269, 497, 782, 911, 1604, 1661
BsmFI GGGAC 1 cut(s) 354
BsnI GGCC 4 cut(s) 42, 176, 583, 1642
Bso31I GGTCTC 3 cut(s) 269, 497, 782
BsoBI CYCGRG 3 cut(s) 262, 1064, 1490
Bsp119I TTCGAA 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 1502
Bsp143I GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1857
Bsp19I CCATGG 2 cut(s) 1350, 1427
BspANI GGCC 4 cut(s) 42, 176, 583, 1642
BspCNI CTCAG 4 cut(s) 13, 266, 783, 1663
BspFNI CGCG 7 cut(s) 486, 1511, 1536, 1583, 1622, 1730, 1813
BspLI GGNNCC 7 cut(s) 271, 342, 422, 1440, 1594, 1714, 2003
BspOI GCTAGC 1 cut(s) 1077
BspPI GGATC 1 cut(s) 557
BspQI GCTCTTC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 282
BspT107I GGYRCC 3 cut(s) 1592, 1712, 2001
BspTI CTTAAG 1 cut(s) 718
BspTNI GGTCTC 3 cut(s) 269, 497, 782
BsrBI CCGCTC 1 cut(s) 1831
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 2 cut(s) 1255, 1589
BsrI ACTGG 2 cut(s) 67, 1635
BssAI RCCGGY 2 cut(s) 1255, 1589
BssECI CCNNGG 7 cut(s) 262, 508, 1350, 1427, 1605, 1644, 1716
BssMI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1857
BssNI GRCGYC 3 cut(s) 309, 1131, 2002
BssSI CACGAG 1 cut(s) 1967
BssT1I CCWWGG 3 cut(s) 508, 1350, 1427
Bst2BI CACGAG 1 cut(s) 1967
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 2 cut(s) 1232, 1978
Bst6I CTCTTC 4 cut(s) 204, 375, 859, 1651
BstACI GRCGYC 3 cut(s) 309, 1131, 2002
BstAFI CTTAAG 1 cut(s) 718
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBAI YACGTR 1 cut(s) 1752
BstBI TTCGAA 1 cut(s) 282
BstDEI CTNAG 5 cut(s) 21, 253, 770, 1078, 1671
BstDSI CCRYGG 3 cut(s) 1350, 1427, 1716
BstEII GGTNACC 1 cut(s) 66
BstF5I GGATG 9 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1897, 1910
BstFNI CGCG 7 cut(s) 486, 1511, 1536, 1583, 1622, 1730, 1813
BstH2I RGCGCY 3 cut(s) 1262, 1358, 2005
BstHHI GCGC 9 cut(s) 356, 486, 1261, 1357, 1513, 1538, 1618, 1624, 2004
BstKTI GATC 8 cut(s) 565, 839, 1087, 1253, 1305, 1458, 1587, 1860
BstMAI GTCTC 6 cut(s) 269, 497, 782, 911, 1604, 1661
BstMBI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1857
BstMCI CGRYCG 2 cut(s) 1587, 1980
BstNI CCWGG 1 cut(s) 776
BstNSI RCATGY 1 cut(s) 103
BstPAI GACNNNNGTC 1 cut(s) 1635
BstPI GGTNACC 1 cut(s) 66
BstSCI CCNGG 6 cut(s) 261, 262, 774, 823, 1604, 1922
BstSLI GKGCMC 1 cut(s) 1502
BstUI CGCG 7 cut(s) 486, 1511, 1536, 1583, 1622, 1730, 1813
BstV1I GCAGC 6 cut(s) 29, 338, 1072, 1344, 1576, 1770
BstV2I GAAGAC 4 cut(s) 102, 399, 711, 1233
BstX2I RGATCY 1 cut(s) 1455
BstYI RGATCY 1 cut(s) 1455
BsuI GTATCC 1 cut(s) 1458
BsuRI GGCC 4 cut(s) 42, 176, 583, 1642
BtgI CCRYGG 3 cut(s) 1350, 1427, 1716
BtsCI GGATG 9 cut(s) 187, 614, 675, 685, 1031, 1318, 1423, 1897, 1910
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 2 cut(s) 60, 242
CaiI CAGNNNCTG 2 cut(s) 1670, 1783
CfoI GCGC 9 cut(s) 356, 486, 1261, 1357, 1513, 1538, 1618, 1624, 2004
Cfr10I RCCGGY 2 cut(s) 1255, 1589
Cfr13I GGNCC 4 cut(s) 341, 582, 712, 1641
Cfr9I CCCGGG 1 cut(s) 262
CseI GACGC 2 cut(s) 298, 475
Csp6I GTAC 3 cut(s) 802, 1593, 1724
CviAII CATG 7 cut(s) 48, 100, 130, 1351, 1428, 1804, 1846
CviQI GTAC 3 cut(s) 802, 1593, 1724
DdeI CTNAG 5 cut(s) 21, 253, 770, 1078, 1671
DinI GGCGCC 1 cut(s) 2003
DpnI GATC 8 cut(s) 564, 838, 1086, 1252, 1304, 1457, 1586, 1859
DpnII GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1857
DrdI GACNNNNNNGTC 1 cut(s) 847
DseDI GACNNNNNNGTC 1 cut(s) 847
Eam1104I CTCTTC 4 cut(s) 204, 375, 859, 1651
EarI CTCTTC 4 cut(s) 204, 375, 859, 1651
EciI GGCGGA 1 cut(s) 2005
Eco130I CCWWGG 3 cut(s) 508, 1350, 1427
Eco31I GGTCTC 3 cut(s) 269, 497, 782
Eco32I GATATC 1 cut(s) 1434
Eco47I GGWCC 2 cut(s) 341, 712
Eco57I CTGAAG 1 cut(s) 1553
Eco72I CACGTG 1 cut(s) 1752
Eco88I CYCGRG 3 cut(s) 262, 1064, 1490
Eco91I GGTNACC 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 774
EcoRV GATATC 1 cut(s) 1434
EcoT14I CCWWGG 3 cut(s) 508, 1350, 1427
EgeI GGCGCC 1 cut(s) 2003
EheI GGCGCC 1 cut(s) 2003
ErhI CCWWGG 3 cut(s) 508, 1350, 1427
FaeI CATG 7 cut(s) 51, 103, 133, 1354, 1431, 1807, 1849
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FaqI GGGAC 1 cut(s) 354
FatI CATG 7 cut(s) 47, 99, 129, 1350, 1427, 1803, 1845
FauI CCCGC 4 cut(s) 1454, 1627, 1806, 1824
FokI GGATG 9 cut(s) 174, 601, 672, 682, 1038, 1325, 1410, 1904, 1917
FspBI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
GlaI GCGC 9 cut(s) 355, 485, 1260, 1356, 1512, 1537, 1617, 1623, 2003
GsuI CTGGAG 1 cut(s) 1618
HaeII RGCGCY 3 cut(s) 1262, 1358, 2005
HaeIII GGCC 4 cut(s) 42, 176, 583, 1642
HgaI GACGC 2 cut(s) 298, 475
HhaI GCGC 9 cut(s) 356, 486, 1261, 1357, 1513, 1538, 1618, 1624, 2004
Hin1I GRCGYC 3 cut(s) 309, 1131, 2002
Hin1II CATG 7 cut(s) 51, 103, 133, 1354, 1431, 1807, 1849
Hin6I GCGC 9 cut(s) 354, 484, 1259, 1355, 1511, 1536, 1616, 1622, 2002
HinP1I GCGC 9 cut(s) 354, 484, 1259, 1355, 1511, 1536, 1616, 1622, 2002
HincII GTYRAC 2 cut(s) 1886, 1920
HindII GTYRAC 2 cut(s) 1886, 1920
HindIII AAGCTT 1 cut(s) 146
HphI GGTGA 1 cut(s) 1867
Hpy166II GTNNAC 5 cut(s) 1500, 1726, 1886, 1920, 1984
Hpy188I TCNGA 7 cut(s) 256, 274, 391, 567, 1300, 1312, 1438
Hpy188III TCNNGA 7 cut(s) 368, 442, 675, 689, 834, 1273, 1945
Hpy8I GTNNAC 5 cut(s) 1500, 1726, 1886, 1920, 1984
Hpy99I CGWCG 8 cut(s) 491, 1133, 1136, 1289, 1385, 1388, 1544, 1978
HpyAV CCTTC 7 cut(s) 289, 944, 1202, 1235, 1700, 1730, 1846
HpyCH4III ACNGT 2 cut(s) 1232, 1978
HpyCH4IV ACGT 2 cut(s) 1131, 1751
HpyCH4V TGCA 7 cut(s) 57, 235, 528, 1500, 1546, 1819, 1938
HpyF3I CTNAG 5 cut(s) 21, 253, 770, 1078, 1671
HpySE526I ACGT 2 cut(s) 1131, 1751
Hsp92I GRCGYC 3 cut(s) 309, 1131, 2002
Hsp92II CATG 7 cut(s) 51, 103, 133, 1354, 1431, 1807, 1849
HspAI GCGC 9 cut(s) 354, 484, 1259, 1355, 1511, 1536, 1616, 1622, 2002
KasI GGCGCC 1 cut(s) 2001
KpnI GGTACC 1 cut(s) 1596
KroI GCCGGC 1 cut(s) 1255
KroNI GCCGGC 1 cut(s) 1257
Kzo9I GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1857
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 9 cut(s) 38, 275, 881, 1177, 1363, 1444, 1578, 1684, 1690
Lsp1109I GCAGC 6 cut(s) 29, 338, 1072, 1344, 1576, 1770
LweI GCATC 5 cut(s) 537, 1254, 1303, 1316, 1828
MaeI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
MaeII ACGT 2 cut(s) 1131, 1751
MaeIII GTNAC 2 cut(s) 66, 1577
MalI GATC 8 cut(s) 564, 838, 1086, 1252, 1304, 1457, 1586, 1859
MbiI CCGCTC 1 cut(s) 1831
MboI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1857
MfeI CAATTG 1 cut(s) 537
MflI RGATCY 1 cut(s) 1455
MhlI GDGCHC 1 cut(s) 1502
MluCI AATT 6 cut(s) 222, 296, 537, 1203, 1277, 1318
Mly113I GGCGCC 1 cut(s) 2002
MlyI GAGTC 5 cut(s) 464, 527, 835, 1466, 1625
MmeI TCCRAC 4 cut(s) 986, 1155, 1770, 1866
MreI CGCCGGCG 1 cut(s) 1255
MroNI GCCGGC 1 cut(s) 1255
MseI TTAA 3 cut(s) 719, 1010, 1280
MslI CAYNNNNRTG 2 cut(s) 83, 523
MspA1I CMGCKG 2 cut(s) 20, 351
MspCI CTTAAG 1 cut(s) 718
MspR9I CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1924
MunI CAATTG 1 cut(s) 537
MvaI CCWGG 1 cut(s) 776
MvnI CGCG 7 cut(s) 486, 1511, 1536, 1583, 1622, 1730, 1813
NaeI GCCGGC 1 cut(s) 1257
NarI GGCGCC 1 cut(s) 2002
NciI CCSGG 5 cut(s) 263, 264, 825, 1606, 1924
NcoI CCATGG 2 cut(s) 1350, 1427
NdeII GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1857
NgoMIV GCCGGC 1 cut(s) 1255
NheI GCTAGC 1 cut(s) 1073
NlaIII CATG 7 cut(s) 51, 103, 133, 1354, 1431, 1807, 1849
NlaIV GGNNCC 7 cut(s) 271, 342, 422, 1440, 1594, 1714, 2003
NmuCI GTSAC 1 cut(s) 1577
NspI RCATGY 1 cut(s) 103
NspV TTCGAA 1 cut(s) 282
OliI CACNNNNGTG 1 cut(s) 523
PaeR7I CTCGAG 2 cut(s) 1064, 1490
PciI ACATGT 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 3 cut(s) 582, 1137, 1970
PdiI GCCGGC 1 cut(s) 1257
PfeI GAWTC 7 cut(s) 628, 692, 828, 1150, 1335, 1774, 1961
PflFI GACNNNGTC 1 cut(s) 710
PfoI TCCNGGA 1 cut(s) 823
Ple19I CGATCG 1 cut(s) 1587
PleI GAGTC 5 cut(s) 463, 527, 835, 1466, 1625
PluTI GGCGCC 1 cut(s) 2005
PmaCI CACGTG 1 cut(s) 1752
PmlI CACGTG 1 cut(s) 1752
PpsI GAGTC 5 cut(s) 463, 527, 835, 1466, 1625
Ppu21I YACGTR 1 cut(s) 1752
PpuMI RGGWCCY 1 cut(s) 341
PscI ACATGT 1 cut(s) 99
PshAI GACNNNNGTC 1 cut(s) 1635
Psp5II RGGWCCY 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 774
PspCI CACGTG 1 cut(s) 1752
PspEI GGTNACC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 774
PspN4I GGNNCC 7 cut(s) 271, 342, 422, 1440, 1594, 1714, 2003
PspPI GGNCC 4 cut(s) 341, 582, 712, 1641
PspPPI RGGWCCY 1 cut(s) 341
PspXI VCTCGAGB 2 cut(s) 1064, 1490
PstNI CAGNNNCTG 2 cut(s) 1670, 1783
PsuI RGATCY 1 cut(s) 1455
PsyI GACNNNGTC 1 cut(s) 710
PvuI CGATCG 1 cut(s) 1587
PvuII CAGCTG 1 cut(s) 20
RgaI GCGATCGC 1 cut(s) 1587
RsaI GTAC 3 cut(s) 803, 1594, 1725
RsaNI GTAC 3 cut(s) 802, 1593, 1724
RseI CAYNNNNRTG 2 cut(s) 83, 523
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 3 cut(s) 719, 1010, 1280
Sau3AI GATC 8 cut(s) 562, 836, 1084, 1250, 1302, 1455, 1584, 1857
Sau96I GGNCC 4 cut(s) 341, 582, 712, 1641
SchI GAGTC 5 cut(s) 464, 527, 835, 1466, 1625
ScrFI CCNGG 6 cut(s) 263, 264, 776, 825, 1606, 1924
SduI GDGCHC 1 cut(s) 1502
SfaAI GCGATCGC 1 cut(s) 1587
SfaNI GCATC 5 cut(s) 537, 1254, 1303, 1316, 1828
SfoI GGCGCC 1 cut(s) 2003
Sfr274I CTCGAG 2 cut(s) 1064, 1490
SfuI TTCGAA 1 cut(s) 282
SgfI GCGATCGC 1 cut(s) 1587
SgrAI CRCCGGYG 1 cut(s) 1255
SinI GGWCC 2 cut(s) 341, 712
SlaI CTCGAG 2 cut(s) 1064, 1490
SmaI CCCGGG 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 83, 523
SmlI CTYRAG 6 cut(s) 429, 718, 832, 1064, 1490, 1826
SmoI CTYRAG 6 cut(s) 429, 718, 832, 1064, 1490, 1826
Sse9I AATT 6 cut(s) 222, 296, 537, 1203, 1277, 1318
SspDI GGCGCC 1 cut(s) 2001
SspMI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
StyD4I CCNGG 6 cut(s) 261, 262, 774, 823, 1604, 1922
StyI CCWWGG 3 cut(s) 508, 1350, 1427
TaaI ACNGT 2 cut(s) 1232, 1978
TaiI ACGT 2 cut(s) 1134, 1754
TasI AATT 6 cut(s) 222, 296, 537, 1203, 1277, 1318
TatI WGTACW 1 cut(s) 801
TauI GCSGC 7 cut(s) 270, 293, 1037, 1511, 1531, 1749, 2002
TfiI GAWTC 7 cut(s) 628, 692, 828, 1150, 1335, 1774, 1961
Tru1I TTAA 3 cut(s) 719, 1010, 1280
Tru9I TTAA 3 cut(s) 719, 1010, 1280
TscAI CASTG 2 cut(s) 67, 242
TseFI GTSAC 1 cut(s) 1577
TseI GCWGC 6 cut(s) 17, 351, 1060, 1357, 1564, 1783
Tsp45I GTSAC 1 cut(s) 1577
TspDTI ATGAA 7 cut(s) 98, 603, 606, 770, 1131, 1233, 1239
TspGWI ACGGA 2 cut(s) 777, 1565
TspMI CCCGGG 1 cut(s) 262
TspRI CASTG 2 cut(s) 67, 242
Tth111I GACNNNGTC 1 cut(s) 710
Vha464I CTTAAG 1 cut(s) 718
VneI GTGCAC 1 cut(s) 1498
VpaK11BI GGWCC 2 cut(s) 341, 712
XapI RAATTY 2 cut(s) 296, 1318
XbaI TCTAGA 2 cut(s) 367, 674
XceI RCATGY 1 cut(s) 103
XhoI CTCGAG 2 cut(s) 1064, 1490
XmaI CCCGGG 1 cut(s) 262
XmaJI CCTAGG 1 cut(s) 508
XspI CTAG 8 cut(s) 203, 368, 509, 675, 873, 1074, 1248, 1469
ZraI GACGTC 1 cut(s) 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.