RchiOBHm_Chr3g0480201

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009219
Physical Location & Seq
Forward (+)
25973362 .. 25977661
4300 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44523

Sequence Viewer

Length: 1152 bp
ATGGGCTCGACCTCTATGACCACTACTGACTCAGGAGGCAGCTCCTCCTCCCCACCCAACAACTCCTCCACCGAGGAGTCCCTCAACGGCCTCAAATTCGGCCGCAAAATCTACTTTGAGGACGCTGCTCTCGGACCCAACCCCCCCAAATCCGGGTCAGGGTCTGCCGGCTCTTCCTCCTCCGCGGCCACGCCGCCCAAGAAGCAGAGGAGCGGTGGTGGTGCTGGGTTGGTCCACCAGGGCCACCCTCCCAGGTGCCAGGTCGAGGGCTGCAACGTAGATCTGAGTGGTGTTAAAGCTTACTATTCCAGGCACAAAGTGTGTGCCATGCACTCCAAGACCCCTACTGTTATTGTTGCCGGCCTTGAACAGAGGTTTTGCCAGCAGTGTAGCAGATTTCATCAGCTTCCTGAATTTGACCAAGGGAAACGTAGTTGCCGTAGACGTCTGGCTGGTCATAATGAGCGTAGGAGAAAGCCACAACCTGGATCCATGATGTCTACGCGTTTTGGCCGTCTCTCTTCGTCAATTTATGAAAACACCAGCCGAGTTGGAAGCTTTCTGATGGACTTCACCGCATACCCAAGGGCTGGGAGAGATGAATGGGCAACTGCAAGAACTTGTGAGCGGCCACCTGGAAATCAAACTGTCACTGCCACCGGAAAGTTTCTTCAACATCCATGGCAGAGCAACCCTGAGAATACTCGATCTGATCTTTACCTGCAAGGGTCAGCCGGTGGTACCAGTTATCCTGGTCCTGGAATTCCTCCGGGAGAATGCGTCTCAGGAGTCACCGACTCGAGCTGTGCTCTCTCTCTTCTGTCAAATCAACCATGGGACTCTAGAAACCGCGGTGTTGGGACAAATGCCTTCATAAACACCCAAGGGGCACCCGTGGCTCAATCAATAGCTAATGTTGGCACCATCAATCCATTTCCAACCACATCATGGGGTTTCAAGGGAAATGAGGCTGGTAGCAGTTCGCATCAGATGCTTCCTGATCTGGGTCTTGGTCAAATTTCGCAGCCTCATAACAGTCATTACTCTAGTGAGCTCGAGTTGTCTCAACAGGGTAGGAGGCAGCAAAATATGGAACTTGGAAACTCCAGGGGCTACGACTCCTCTAGTCAACACATGCATTGGTCTCTTTAA

Protein Analysis

383

Amino Acids

41.13

Weight (kDa)

9.34

Isoelectric Point (pI)

57.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 86 - 159 1e-33 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 448
Acc36I ACCTGC 1 cut(s) 729
Acc65I GGTACC 1 cut(s) 740
AccB1I GGYRCC 4 cut(s) 255, 740, 889, 920
AccB7I CCANNNNNTGG 3 cut(s) 485, 590, 948
AccBSI CCGCTC 2 cut(s) 213, 628
AccI GTMKAC 2 cut(s) 442, 500
AccII CGCG 3 cut(s) 185, 505, 852
AciI CCGC 9 cut(s) 103, 183, 185, 194, 213, 576, 628, 850, 852
AclWI GGATC 2 cut(s) 483, 496
AcoI YGGCCR 4 cut(s) 100, 186, 511, 629
AcsI RAATTY 4 cut(s) 95, 413, 762, 1017
AcyI GRCGYC 1 cut(s) 445
AdeI CACNNNGTG 1 cut(s) 319
AfaI GTAC 1 cut(s) 742
AfiI CCNNNNNNNGG 9 cut(s) 152, 153, 159, 265, 485, 590, 758, 948, 1004
AflIII ACRYGT 1 cut(s) 503
AgsI TTSAA 3 cut(s) 368, 674, 958
AjnI CCWGG 9 cut(s) 237, 251, 258, 308, 484, 634, 751, 757, 1106
AluBI AGCT 7 cut(s) 42, 299, 406, 558, 804, 911, 1054
AluI AGCT 7 cut(s) 42, 299, 406, 558, 804, 911, 1054
Alw21I GWGCWC 2 cut(s) 811, 1056
Alw26I GTCTC 3 cut(s) 521, 787, 1068
AlwI GGATC 2 cut(s) 483, 496
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 2 cut(s) 799, 1055
AoxI GGCC 7 cut(s) 88, 100, 186, 241, 361, 511, 629
ApeKI GCWGC 5 cut(s) 39, 125, 270, 1024, 1081
ApoI RAATTY 4 cut(s) 95, 413, 762, 1017
Asp718I GGTACC 1 cut(s) 740
AspS9I GGNCC 4 cut(s) 134, 232, 241, 755
AsuC2I CCSGG 2 cut(s) 154, 771
AsuHPI GGTGA 2 cut(s) 565, 784
AvaI CYCGRG 2 cut(s) 799, 1055
AvaII GGWCC 3 cut(s) 134, 232, 755
BaeGI GKGCMC 1 cut(s) 892
BamHI GGATCC 1 cut(s) 488
BanI GGYRCC 4 cut(s) 255, 740, 889, 920
BanII GRGCYC 2 cut(s) 8, 1056
Bbv12I GWGCWC 2 cut(s) 811, 1056
BbvI GCAGC 5 cut(s) 51, 112, 257, 1036, 1093
BccI CCATC 2 cut(s) 559, 932
BceAI ACGGC 3 cut(s) 103, 423, 498
BciT130I CCWGG 9 cut(s) 239, 253, 260, 310, 486, 636, 753, 759, 1108
BcnI CCSGG 2 cut(s) 154, 771
BcoDI GTCTC 3 cut(s) 521, 787, 1068
BfaI CTAG 3 cut(s) 843, 1047, 1125
BfuAI ACCTGC 1 cut(s) 729
BglII AGATCT 1 cut(s) 280
BisI GCNGC 9 cut(s) 40, 103, 126, 186, 194, 271, 629, 1025, 1082
BlsI GCNGC 9 cut(s) 41, 104, 127, 187, 195, 272, 630, 1026, 1083
Bme18I GGWCC 3 cut(s) 134, 232, 755
BmeT110I CYCGRG 2 cut(s) 799, 1055
BmgT120I GGNCC 4 cut(s) 134, 232, 241, 755
BmiI GGNNCC 6 cut(s) 136, 257, 490, 742, 891, 922
BmsI GCATC 2 cut(s) 981, 994
BplI GAGNNNNNCTC 2 cut(s) 793, 825
BpmI CTGGAG 1 cut(s) 1090
BpuMI CCSGG 2 cut(s) 154, 771
BsaHI GRCGYC 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 659
BsaXI ACNNNNNCTCC 6 cut(s) 50, 80, 202, 232, 765, 795
Bsc4I CCNNNNNNNGG 9 cut(s) 152, 153, 159, 265, 485, 590, 758, 948, 1004
Bse118I RCCGGY 3 cut(s) 167, 359, 734
Bse1I ACTGG 1 cut(s) 744
BseBI CCWGG 9 cut(s) 239, 253, 260, 310, 486, 636, 753, 759, 1108
BseGI GGATG 1 cut(s) 676
BseLI CCNNNNNNNGG 9 cut(s) 152, 153, 159, 265, 485, 590, 758, 948, 1004
BseMII CTCAG 4 cut(s) 45, 275, 687, 798
BseNI ACTGG 1 cut(s) 744
BseRI GAGGAG 7 cut(s) 34, 37, 55, 89, 169, 223, 1111
BseSI GKGCMC 1 cut(s) 892
BseX3I CGGCCG 1 cut(s) 100
BseXI GCAGC 5 cut(s) 51, 112, 257, 1036, 1093
BseYI CCCAGC 2 cut(s) 224, 590
Bsh1236I CGCG 3 cut(s) 185, 505, 852
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 7 cut(s) 90, 102, 188, 243, 363, 513, 631
BshNI GGYRCC 4 cut(s) 255, 740, 889, 920
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 2 cut(s) 811, 1056
BsiHKCI CYCGRG 2 cut(s) 799, 1055
BsiSI CCGG 6 cut(s) 153, 168, 360, 660, 735, 770
BslFI GGGAC 3 cut(s) 64, 851, 874
BslI CCNNNNNNNGG 9 cut(s) 152, 153, 159, 265, 485, 590, 758, 948, 1004
BsmAI GTCTC 3 cut(s) 521, 787, 1068
BsmBI CGTCTC 2 cut(s) 521, 787
BsmFI GGGAC 3 cut(s) 64, 851, 874
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 7 cut(s) 90, 102, 188, 243, 363, 513, 631
BsoBI CYCGRG 2 cut(s) 799, 1055
Bsp1286I GDGCHC 4 cut(s) 8, 811, 892, 1056
Bsp143I GATC 5 cut(s) 280, 488, 707, 712, 1000
Bsp19I CCATGG 2 cut(s) 680, 833
BspACI CCGC 9 cut(s) 103, 183, 185, 194, 213, 576, 628, 850, 852
BspANI GGCC 7 cut(s) 90, 102, 188, 243, 363, 513, 631
BspCNI CTCAG 4 cut(s) 44, 276, 688, 797
BspFNI CGCG 3 cut(s) 185, 505, 852
BspLI GGNNCC 6 cut(s) 136, 257, 490, 742, 891, 922
BspMI ACCTGC 1 cut(s) 729
BspPI GGATC 2 cut(s) 483, 496
BspQI GCTCTTC 1 cut(s) 178
BspT107I GGYRCC 4 cut(s) 255, 740, 889, 920
BsrBI CCGCTC 2 cut(s) 213, 628
BsrFI RCCGGY 3 cut(s) 167, 359, 734
BsrI ACTGG 1 cut(s) 744
BssAI RCCGGY 3 cut(s) 167, 359, 734
BssMI GATC 5 cut(s) 280, 488, 707, 712, 1000
BssNI GRCGYC 1 cut(s) 445
BssT1I CCWWGG 5 cut(s) 421, 584, 680, 833, 883
Bst2UI CCWGG 9 cut(s) 239, 253, 260, 310, 486, 636, 753, 759, 1108
Bst4CI ACNGT 3 cut(s) 349, 649, 1037
Bst6I CTCTTC 3 cut(s) 178, 526, 822
BstACI GRCGYC 1 cut(s) 445
BstAPI GCANNNNNTGC 1 cut(s) 991
BstC8I GCNNGC 3 cut(s) 169, 361, 383
BstDEI CTNAG 4 cut(s) 31, 284, 696, 784
BstDSI CCRYGG 5 cut(s) 183, 680, 833, 850, 894
BstF5I GGATG 1 cut(s) 676
BstFNI CGCG 3 cut(s) 185, 505, 852
BstKTI GATC 5 cut(s) 283, 491, 710, 715, 1003
BstMAI GTCTC 3 cut(s) 521, 787, 1068
BstMBI GATC 5 cut(s) 280, 488, 707, 712, 1000
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 3 cut(s) 202, 896, 991
BstNI CCWGG 9 cut(s) 239, 253, 260, 310, 486, 636, 753, 759, 1108
BstNSI RCATGY 1 cut(s) 1138
BstSLI GKGCMC 1 cut(s) 892
BstUI CGCG 3 cut(s) 185, 505, 852
BstV1I GCAGC 5 cut(s) 51, 112, 257, 1036, 1093
BstX2I RGATCY 2 cut(s) 280, 488
BstYI RGATCY 2 cut(s) 280, 488
BstZI CGGCCG 1 cut(s) 100
BsuRI GGCC 7 cut(s) 90, 102, 188, 243, 363, 513, 631
BtgI CCRYGG 5 cut(s) 183, 680, 833, 850, 894
BtsCI GGATG 1 cut(s) 676
BtsI GCAGTG 2 cut(s) 392, 651
BtsIMutI CAGTG 2 cut(s) 392, 651
BveI ACCTGC 1 cut(s) 729
Cac8I GCNNGC 3 cut(s) 169, 361, 383
CaiI CAGNNNCTG 1 cut(s) 164
Cfr10I RCCGGY 3 cut(s) 167, 359, 734
Cfr13I GGNCC 4 cut(s) 134, 232, 241, 755
Cfr42I CCGCGG 2 cut(s) 186, 853
CseI GACGC 2 cut(s) 131, 769
Csp6I GTAC 1 cut(s) 741
CviAII CATG 6 cut(s) 328, 493, 681, 834, 948, 1135
CviQI GTAC 1 cut(s) 741
DdeI CTNAG 4 cut(s) 31, 284, 696, 784
DpnI GATC 5 cut(s) 282, 490, 709, 714, 1002
DpnII GATC 5 cut(s) 280, 488, 707, 712, 1000
DraIII CACNNNGTG 1 cut(s) 319
EaeI YGGCCR 4 cut(s) 100, 186, 511, 629
EagI CGGCCG 1 cut(s) 100
Eam1104I CTCTTC 3 cut(s) 178, 526, 822
EarI CTCTTC 3 cut(s) 178, 526, 822
Ecl136II GAGCTC 1 cut(s) 1054
EclXI CGGCCG 1 cut(s) 100
Eco130I CCWWGG 5 cut(s) 421, 584, 680, 833, 883
Eco24I GRGCYC 2 cut(s) 8, 1056
Eco47I GGWCC 3 cut(s) 134, 232, 755
Eco52I CGGCCG 1 cut(s) 100
Eco53kI GAGCTC 1 cut(s) 1054
Eco88I CYCGRG 2 cut(s) 799, 1055
EcoICRI GAGCTC 1 cut(s) 1054
EcoRI GAATTC 1 cut(s) 762
EcoRII CCWGG 9 cut(s) 237, 251, 258, 308, 484, 634, 751, 757, 1106
EcoT14I CCWWGG 5 cut(s) 421, 584, 680, 833, 883
EcoT22I ATGCAT 1 cut(s) 1140
EcoT38I GRGCYC 2 cut(s) 8, 1056
ErhI CCWWGG 5 cut(s) 421, 584, 680, 833, 883
Esp3I CGTCTC 2 cut(s) 521, 787
FaeI CATG 6 cut(s) 331, 496, 684, 837, 951, 1138
FaqI GGGAC 3 cut(s) 64, 851, 874
FatI CATG 6 cut(s) 327, 492, 680, 833, 947, 1134
FblI GTMKAC 2 cut(s) 442, 500
Fnu4HI GCNGC 9 cut(s) 40, 103, 126, 186, 194, 271, 629, 1025, 1082
FokI GGATG 1 cut(s) 663
FriOI GRGCYC 2 cut(s) 8, 1056
Fsp4HI GCNGC 9 cut(s) 40, 103, 126, 186, 194, 271, 629, 1025, 1082
FspBI CTAG 3 cut(s) 843, 1047, 1125
GluI GCNGC 9 cut(s) 40, 103, 126, 186, 194, 271, 629, 1025, 1082
GsaI CCCAGC 2 cut(s) 228, 594
GsuI CTGGAG 1 cut(s) 1090
HaeIII GGCC 7 cut(s) 90, 102, 188, 243, 363, 513, 631
HapII CCGG 6 cut(s) 153, 168, 360, 660, 735, 770
HgaI GACGC 2 cut(s) 131, 769
Hin1I GRCGYC 1 cut(s) 445
Hin1II CATG 6 cut(s) 331, 496, 684, 837, 951, 1138
HincII GTYRAC 1 cut(s) 1130
HindII GTYRAC 1 cut(s) 1130
HindIII AAGCTT 2 cut(s) 297, 556
HinfI GANTC 6 cut(s) 29, 77, 789, 797, 839, 1118
HpaII CCGG 6 cut(s) 153, 168, 360, 660, 735, 770
HphI GGTGA 2 cut(s) 565, 784
Hpy166II GTNNAC 4 cut(s) 235, 443, 501, 1130
Hpy188I TCNGA 5 cut(s) 134, 285, 564, 712, 990
Hpy188III TCNNGA 5 cut(s) 33, 410, 786, 843, 998
Hpy8I GTNNAC 4 cut(s) 235, 443, 501, 1130
HpyAV CCTTC 1 cut(s) 880
HpyCH4III ACNGT 3 cut(s) 349, 649, 1037
HpyCH4IV ACGT 3 cut(s) 276, 430, 445
HpyCH4V TGCA 5 cut(s) 273, 331, 614, 724, 1138
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 896, 991
HpyF3I CTNAG 4 cut(s) 31, 284, 696, 784
HpySE526I ACGT 3 cut(s) 276, 430, 445
Hsp92I GRCGYC 1 cut(s) 445
Hsp92II CATG 6 cut(s) 331, 496, 684, 837, 951, 1138
KpnI GGTACC 1 cut(s) 744
KroI GCCGGC 2 cut(s) 167, 359
KroNI GCCGGC 2 cut(s) 169, 361
KspI CCGCGG 2 cut(s) 186, 853
Kzo9I GATC 5 cut(s) 280, 488, 707, 712, 1000
LguI GCTCTTC 1 cut(s) 178
LmnI GCTCC 2 cut(s) 47, 210
Lsp1109I GCAGC 5 cut(s) 51, 112, 257, 1036, 1093
LweI GCATC 2 cut(s) 981, 994
MaeI CTAG 3 cut(s) 843, 1047, 1125
MaeII ACGT 3 cut(s) 276, 430, 445
MaeIII GTNAC 2 cut(s) 649, 790
MalI GATC 5 cut(s) 282, 490, 709, 714, 1002
MbiI CCGCTC 2 cut(s) 213, 628
MboI GATC 5 cut(s) 280, 488, 707, 712, 1000
MboII GAAGA 4 cut(s) 165, 513, 662, 809
MflI RGATCY 2 cut(s) 280, 488
MhlI GDGCHC 4 cut(s) 8, 811, 892, 1056
MluCI AATT 5 cut(s) 95, 413, 528, 762, 1017
MluI ACGCGT 1 cut(s) 503
MlyI GAGTC 6 cut(s) 23, 86, 791, 798, 833, 1112
MmeI TCCRAC 2 cut(s) 532, 962
Mph1103I ATGCAT 1 cut(s) 1140
MroNI GCCGGC 2 cut(s) 167, 359
MseI TTAA 2 cut(s) 294, 1150
MspA1I CMGCKG 2 cut(s) 185, 852
MspI CCGG 6 cut(s) 153, 168, 360, 660, 735, 770
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 9 cut(s) 239, 253, 260, 310, 486, 636, 753, 759, 1108
MvnI CGCG 3 cut(s) 185, 505, 852
MwoI GCNNNNNNNGC 3 cut(s) 202, 896, 991
NaeI GCCGGC 2 cut(s) 169, 361
NciI CCSGG 2 cut(s) 154, 771
NcoI CCATGG 2 cut(s) 680, 833
NdeII GATC 5 cut(s) 280, 488, 707, 712, 1000
NgoMIV GCCGGC 2 cut(s) 167, 359
NlaIII CATG 6 cut(s) 331, 496, 684, 837, 951, 1138
NlaIV GGNNCC 6 cut(s) 136, 257, 490, 742, 891, 922
NmeAIII GCCGAG 1 cut(s) 572
NmuCI GTSAC 2 cut(s) 649, 790
NsiI ATGCAT 1 cut(s) 1140
NspI RCATGY 1 cut(s) 1138
PaeR7I CTCGAG 2 cut(s) 799, 1055
PciSI GCTCTTC 1 cut(s) 178
PcsI WCGNNNNNNNCGW 1 cut(s) 436
PctI GAATGC 1 cut(s) 782
PdiI GCCGGC 2 cut(s) 169, 361
PflMI CCANNNNNTGG 3 cut(s) 485, 590, 948
PfoI TCCNGGA 2 cut(s) 757, 769
PkrI GCNGC 9 cut(s) 41, 104, 127, 187, 195, 272, 630, 1026, 1083
PleI GAGTC 6 cut(s) 23, 85, 791, 797, 833, 1112
PpsI GAGTC 6 cut(s) 23, 85, 791, 797, 833, 1112
Psp124BI GAGCTC 1 cut(s) 1056
Psp6I CCWGG 9 cut(s) 237, 251, 258, 308, 484, 634, 751, 757, 1106
PspFI CCCAGC 2 cut(s) 224, 590
PspGI CCWGG 9 cut(s) 237, 251, 258, 308, 484, 634, 751, 757, 1106
PspN4I GGNNCC 6 cut(s) 136, 257, 490, 742, 891, 922
PspPI GGNCC 4 cut(s) 134, 232, 241, 755
PspXI VCTCGAGB 2 cut(s) 799, 1055
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 2 cut(s) 280, 488
RsaI GTAC 1 cut(s) 742
RsaNI GTAC 1 cut(s) 741
SacI GAGCTC 1 cut(s) 1056
SacII CCGCGG 2 cut(s) 186, 853
SapI GCTCTTC 1 cut(s) 178
SaqAI TTAA 2 cut(s) 294, 1150
SatI GCNGC 9 cut(s) 40, 103, 126, 186, 194, 271, 629, 1025, 1082
Sau3AI GATC 5 cut(s) 280, 488, 707, 712, 1000
Sau96I GGNCC 4 cut(s) 134, 232, 241, 755
SchI GAGTC 6 cut(s) 23, 86, 791, 798, 833, 1112
SduI GDGCHC 4 cut(s) 8, 811, 892, 1056
SfaNI GCATC 2 cut(s) 981, 994
Sfr274I CTCGAG 2 cut(s) 799, 1055
Sfr303I CCGCGG 2 cut(s) 186, 853
SgrBI CCGCGG 2 cut(s) 186, 853
SinI GGWCC 3 cut(s) 134, 232, 755
SlaI CTCGAG 2 cut(s) 799, 1055
SmlI CTYRAG 2 cut(s) 799, 1055
SmoI CTYRAG 2 cut(s) 799, 1055
Sse9I AATT 5 cut(s) 95, 413, 528, 762, 1017
SsiI CCGC 9 cut(s) 103, 183, 185, 194, 213, 576, 628, 850, 852
SspMI CTAG 3 cut(s) 843, 1047, 1125
SstI GAGCTC 1 cut(s) 1056
StyI CCWWGG 5 cut(s) 421, 584, 680, 833, 883
TaaI ACNGT 3 cut(s) 349, 649, 1037
TaiI ACGT 3 cut(s) 279, 433, 448
TaqI TCGA 5 cut(s) 8, 264, 706, 800, 1056
TasI AATT 5 cut(s) 95, 413, 528, 762, 1017
TauI GCSGC 4 cut(s) 105, 188, 196, 631
Tru1I TTAA 2 cut(s) 294, 1150
Tru9I TTAA 2 cut(s) 294, 1150
TscAI CASTG 2 cut(s) 392, 658
TseFI GTSAC 2 cut(s) 649, 790
TseI GCWGC 5 cut(s) 39, 125, 270, 1024, 1081
Tsp45I GTSAC 2 cut(s) 649, 790
TspDTI ATGAA 4 cut(s) 389, 549, 615, 862
TspRI CASTG 2 cut(s) 392, 658
Van91I CCANNNNNTGG 3 cut(s) 485, 590, 948
VpaK11BI GGWCC 3 cut(s) 134, 232, 755
XapI RAATTY 4 cut(s) 95, 413, 762, 1017
XbaI TCTAGA 1 cut(s) 842
XceI RCATGY 1 cut(s) 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 945
XhoI CTCGAG 2 cut(s) 799, 1055
XmiI GTMKAC 2 cut(s) 442, 500
XspI CTAG 3 cut(s) 843, 1047, 1125
ZraI GACGTC 1 cut(s) 446
Zsp2I ATGCAT 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.