RchiOBHm_Chr4g0384821

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012691
Physical Location & Seq
Forward (+)
202224 .. 204473
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35837

Sequence Viewer

Length: 738 bp
ATGCCCTGCTCGTTCATTCAAAGAACTTGTCTTGTTGGAGCTGGTCCTGATGGGAAACACACTGCATTAGAAGCGGTTACCATAGTTCTCGATTTACCACCACCACAACCTGGGTCAATGTTGGGCCTTACATCAGTTGGCAGGGTATCTGCTTCTGATCCAAAGGCAGCCCTGGCCTTACAGAGATTAGTACAAGAAGCAGTATGGTTCCTTGGAGAAAATGCAAATTATGCTGCTTCTGAATATGCTTGGGAATCTGCAACCCCTCCTGGTACCGCATTGATGATGCTAGATACAGATAAAATGGTTGCTGCTGCCAGTTCCCGCAACCCTACTCTAGCTGGTGCATTGACTAGGCTACAGAGGTGTGCATTCAGTGGCAGCTGGGAGGTTCGGATCATTGCTGCTCAAGCTCTCACAACTATGGCAATAAGAGCTGGTGAGCCTTTTAGGCTACAAATCTATCAATTTTTACACACTATAGCAGAGGGCGGAGTGCAGTCTCAGTTTTCTGAGATGCATCCCAGTAATGGAGAAGACCAGGGAGCTAGTGGCACAGGCCTCGGAGTTCTAATCAGTCCCATGATAGAGGTTCTTGATGAAATGTATAGGGCACAAGATGATTTGATCAAGGAAATGCGCAACCATGATAATGCTAATAAGGAATGGAGTCCAAAACAGGATACGACGTATTCAGACCGTATCCAAAACAGGATCCGCGTATCCACACATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

245

Amino Acids

26.59

Weight (kDa)

5.38

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 641
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 110
AccII CGCG 1 cut(s) 720
AciI CCGC 5 cut(s) 74, 276, 325, 492, 718
AclWI GGATC 4 cut(s) 152, 404, 709, 722
AfaI GTAC 2 cut(s) 192, 274
AfiI CCNNNNNNNGG 2 cut(s) 110, 530
AgsI TTSAA 1 cut(s) 20
AjnI CCWGG 4 cut(s) 109, 171, 268, 540
AluBI AGCT 6 cut(s) 41, 341, 384, 413, 437, 548
AluI AGCT 6 cut(s) 41, 341, 384, 413, 437, 548
Alw26I GTCTC 1 cut(s) 507
AlwI GGATC 4 cut(s) 152, 404, 709, 722
AoxI GGCC 3 cut(s) 124, 174, 559
ApeKI GCWGC 6 cut(s) 167, 233, 311, 314, 381, 404
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
Asp718I GGTACC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 642
AspS9I GGNCC 2 cut(s) 44, 124
AsuHPI GGTGA 1 cut(s) 452
AvaII GGWCC 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 616
BamHI GGATCC 1 cut(s) 714
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 543
BbvI GCAGC 6 cut(s) 179, 220, 298, 301, 391, 393
BccI CCATC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 4 cut(s) 111, 173, 270, 542
BciVI GTATCC 3 cut(s) 676, 713, 733
BclI TGATCA 1 cut(s) 627
BcoDI GTCTC 1 cut(s) 507
BfaI CTAG 4 cut(s) 290, 338, 354, 549
BfmI CTRYAG 2 cut(s) 359, 480
BfuI GTATCC 3 cut(s) 676, 713, 733
BglI GCCNNNNNGGC 1 cut(s) 451
BisI GCNGC 6 cut(s) 168, 234, 312, 315, 382, 405
BlsI GCNGC 6 cut(s) 169, 235, 313, 316, 383, 406
Bme1390I CCNGG 4 cut(s) 111, 173, 270, 542
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 44, 124
BmiI GGNNCC 3 cut(s) 209, 274, 716
BmrFI CCNGG 4 cut(s) 111, 173, 270, 542
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 3 cut(s) 276, 507, 529
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 393
BsaJI CCNNGG 5 cut(s) 110, 171, 211, 541, 562
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 530
Bse1I ACTGG 2 cut(s) 318, 525
Bse3DI GCAATG 1 cut(s) 399
BseBI CCWGG 4 cut(s) 111, 173, 270, 542
BseDI CCNNGG 5 cut(s) 110, 171, 211, 541, 562
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 2 cut(s) 110, 530
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 504, 518
BseNI ACTGG 2 cut(s) 318, 525
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 6 cut(s) 179, 220, 298, 301, 391, 393
BseYI CCCAGC 1 cut(s) 384
BsgI GTGCAG 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 720
BshFI GGCC 3 cut(s) 126, 176, 561
BshNI GGYRCC 1 cut(s) 272
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 110, 530
BsmAI GTCTC 1 cut(s) 507
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 3 cut(s) 126, 176, 561
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 4 cut(s) 157, 396, 627, 714
BspACI CCGC 5 cut(s) 74, 276, 325, 492, 718
BspANI GGCC 3 cut(s) 126, 176, 561
BspCNI CTCAG 2 cut(s) 505, 517
BspFNI CGCG 1 cut(s) 720
BspLI GGNNCC 3 cut(s) 209, 274, 716
BspPI GGATC 4 cut(s) 152, 404, 709, 722
BspT107I GGYRCC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 2 cut(s) 318, 525
BssECI CCNNGG 5 cut(s) 110, 171, 211, 541, 562
BssMI GATC 4 cut(s) 157, 396, 627, 714
BssT1I CCWWGG 1 cut(s) 211
Bst2UI CCWGG 4 cut(s) 111, 173, 270, 542
Bst4CI ACNGT 1 cut(s) 701
BstAPI GCANNNNNTGC 1 cut(s) 230
BstDEI CTNAG 2 cut(s) 504, 513
BstEII GGTNACC 1 cut(s) 76
BstF5I GGATG 1 cut(s) 520
BstFNI CGCG 1 cut(s) 720
BstHHI GCGC 1 cut(s) 642
BstKTI GATC 4 cut(s) 160, 399, 630, 717
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 4 cut(s) 157, 396, 627, 714
BstMWI GCNNNNNNNGC 6 cut(s) 71, 173, 230, 410, 434, 451
BstNI CCWGG 4 cut(s) 111, 173, 270, 542
BstPI GGTNACC 1 cut(s) 76
BstSCI CCNGG 4 cut(s) 109, 171, 268, 540
BstSFI CTRYAG 2 cut(s) 359, 480
BstSLI GKGCMC 1 cut(s) 616
BstUI CGCG 1 cut(s) 720
BstV1I GCAGC 6 cut(s) 179, 220, 298, 301, 391, 393
BstV2I GAAGAC 1 cut(s) 543
BstX2I RGATCY 1 cut(s) 714
BstYI RGATCY 1 cut(s) 714
BsuI GTATCC 3 cut(s) 676, 713, 733
BsuRI GGCC 3 cut(s) 126, 176, 561
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 60, 382
CfoI GCGC 1 cut(s) 642
Cfr13I GGNCC 2 cut(s) 44, 124
Csp6I GTAC 2 cut(s) 191, 273
CviAII CATG 2 cut(s) 583, 647
CviQI GTAC 2 cut(s) 191, 273
DdeI CTNAG 2 cut(s) 504, 513
DpnI GATC 4 cut(s) 159, 398, 629, 716
DpnII GATC 4 cut(s) 157, 396, 627, 714
EciI GGCGGA 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 211
Eco147I AGGCCT 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 76
EcoO65I GGTNACC 1 cut(s) 76
EcoRII CCWGG 4 cut(s) 109, 171, 268, 540
EcoT14I CCWWGG 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 211
FaeI CATG 2 cut(s) 586, 650
FaqI GGGAC 1 cut(s) 564
FatI CATG 2 cut(s) 582, 646
FauI CCCGC 1 cut(s) 332
FbaI TGATCA 1 cut(s) 627
Fnu4HI GCNGC 6 cut(s) 168, 234, 312, 315, 382, 405
FokI GGATG 1 cut(s) 507
Fsp4HI GCNGC 6 cut(s) 168, 234, 312, 315, 382, 405
FspBI CTAG 4 cut(s) 290, 338, 354, 549
FspI TGCGCA 1 cut(s) 641
GlaI GCGC 1 cut(s) 641
GluI GCNGC 6 cut(s) 168, 234, 312, 315, 382, 405
GsaI CCCAGC 1 cut(s) 388
HaeIII GGCC 3 cut(s) 126, 176, 561
HhaI GCGC 1 cut(s) 642
Hin1II CATG 2 cut(s) 586, 650
Hin6I GCGC 1 cut(s) 640
HinP1I GCGC 1 cut(s) 640
HinfI GANTC 2 cut(s) 254, 670
HphI GGTGA 1 cut(s) 452
Hpy188I TCNGA 7 cut(s) 157, 241, 396, 514, 566, 697, 737
Hpy188III TCNNGA 3 cut(s) 47, 89, 596
Hpy99I CGWCG 1 cut(s) 691
HpyCH4III ACNGT 1 cut(s) 701
HpyCH4IV ACGT 1 cut(s) 689
HpyCH4V TGCA 7 cut(s) 65, 224, 260, 347, 371, 499, 520
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 173, 230, 410, 434, 451
HpyF3I CTNAG 2 cut(s) 504, 513
HpySE526I ACGT 1 cut(s) 689
Hsp92II CATG 2 cut(s) 586, 650
HspAI GCGC 1 cut(s) 640
KpnI GGTACC 1 cut(s) 276
Ksp22I TGATCA 1 cut(s) 627
Kzo9I GATC 4 cut(s) 157, 396, 627, 714
LmnI GCTCC 2 cut(s) 38, 545
Lsp1109I GCAGC 6 cut(s) 179, 220, 298, 301, 391, 393
LweI GCATC 3 cut(s) 276, 507, 529
MaeI CTAG 4 cut(s) 290, 338, 354, 549
MaeII ACGT 1 cut(s) 689
MaeIII GTNAC 1 cut(s) 76
MalI GATC 4 cut(s) 159, 398, 629, 716
MboI GATC 4 cut(s) 157, 396, 627, 714
MboII GAAGA 1 cut(s) 548
MflI RGATCY 1 cut(s) 714
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 2 cut(s) 226, 467
MlyI GAGTC 1 cut(s) 679
MmeI TCCRAC 1 cut(s) 16
MnlI CCTC 6 cut(s) 276, 357, 382, 481, 572, 583
Mph1103I ATGCAT 1 cut(s) 522
MslI CAYNNNNRTG 2 cut(s) 422, 651
MspA1I CMGCKG 1 cut(s) 384
MspR9I CCNGG 4 cut(s) 111, 173, 270, 542
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 4 cut(s) 111, 173, 270, 542
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 6 cut(s) 71, 173, 230, 410, 434, 451
NdeII GATC 4 cut(s) 157, 396, 627, 714
NlaIII CATG 2 cut(s) 586, 650
NlaIV GGNNCC 3 cut(s) 209, 274, 716
NsbI TGCGCA 1 cut(s) 641
NsiI ATGCAT 1 cut(s) 522
PceI AGGCCT 1 cut(s) 561
PctI GAATGC 1 cut(s) 371
PfeI GAWTC 1 cut(s) 254
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 6 cut(s) 169, 235, 313, 316, 383, 406
PleI GAGTC 1 cut(s) 678
PpsI GAGTC 1 cut(s) 678
Psp6I CCWGG 4 cut(s) 109, 171, 268, 540
PspEI GGTNACC 1 cut(s) 76
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 4 cut(s) 109, 171, 268, 540
PspN4I GGNNCC 3 cut(s) 209, 274, 716
PspPI GGNCC 2 cut(s) 44, 124
PsuI RGATCY 1 cut(s) 714
PvuII CAGCTG 1 cut(s) 384
RsaI GTAC 2 cut(s) 192, 274
RsaNI GTAC 2 cut(s) 191, 273
RseI CAYNNNNRTG 2 cut(s) 422, 651
SatI GCNGC 6 cut(s) 168, 234, 312, 315, 382, 405
Sau3AI GATC 4 cut(s) 157, 396, 627, 714
Sau96I GGNCC 2 cut(s) 44, 124
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 4 cut(s) 111, 173, 270, 542
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 3 cut(s) 276, 507, 529
SfcI CTRYAG 2 cut(s) 359, 480
SinI GGWCC 1 cut(s) 44
SmiMI CAYNNNNRTG 2 cut(s) 422, 651
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
Sse9I AATT 2 cut(s) 226, 467
SseBI AGGCCT 1 cut(s) 561
SsiI CCGC 5 cut(s) 74, 276, 325, 492, 718
SspMI CTAG 4 cut(s) 290, 338, 354, 549
StuI AGGCCT 1 cut(s) 561
StyD4I CCNGG 4 cut(s) 109, 171, 268, 540
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 1 cut(s) 701
TaiI ACGT 1 cut(s) 692
TaqI TCGA 1 cut(s) 90
TasI AATT 2 cut(s) 226, 467
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 1 cut(s) 254
TscAI CASTG 2 cut(s) 67, 382
TseI GCWGC 6 cut(s) 167, 233, 311, 314, 381, 404
TspDTI ATGAA 2 cut(s) 4, 615
TspRI CASTG 2 cut(s) 67, 382
Van91I CCANNNNNTGG 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 44
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XspI CTAG 4 cut(s) 290, 338, 354, 549
Zsp2I ATGCAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.