Rh4AG002000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0012691
Physical Location & Seq
Reverse (-)
321321 .. 324851
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG002000.1

Sequence Viewer

Length: 801 bp
ATGCATCAACGCCCGGGACACAACTCGCGTCGGCGGTGTCATTGCCGGGAGAGGAGAAAGGGGCGAACCGAAAGGGAGTCTGATAGCCAATTTTATGATTTCAAAATATCTGATGTAAGGAATGAGAATGTGAGAGAGCAGAGGGCTGCAAGGAAGACAATTCAGAGGAGGGAGAGGAGAACCGAAGGGGTATCTGTGAGGCAGCATCTTGAAGATGGAATGGCTCAAATGAGGAAGGAGCAGAATGAGAGGAGTAAACAGAGGGCCGCAAGGAGGGGAAGGGATGCAAGGACTAGAATTGAGAATCAGCTGGCATACGAATCAAGACCAAAACGGAGGGATACTCAGAGGGATTCAGAAAAGAAGGAACTAGGCTTGGTTCGCCAGCAAGAAATGAATGCAAGGCGCAGGGAGGACCTTTTAAATGAGAGGCGAGCACTCACACGGGCAGACATCAGGATGGAATTACAGAGGGAGCGCCTGAAAGATCAGAGAATAAAATGGCATAAGGACTTGGATGACCTAACGGATGAGAGACAGCAGCTCCGGAAACGAAAGGCTTCTTTCAAGAAACTGTGGTGGATTTCCATAGTGGTGGACGGAGTAATTTTAACTTGCTTGGATTTATACATGGAAGACGCAGGTGGCATACGCTATTGGACCTCTTTTGTTGCTTACCTGATACTTTTTTCACTATGGTGGGGTATAATATCCAAGAGACAACAGAAGCTAGAAGGGCCTCCCCAGGCAATTGAGTGTGGAGCTGGATTAATGAAGGGTCTTTGTGTGATCGCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

266

Amino Acids

32.0

Weight (kDa)

10.45

Isoelectric Point (pI)

69.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 632
Acc36I ACCTGC 1 cut(s) 632
AccII CGCG 1 cut(s) 28
AccIII TCCGGA 1 cut(s) 546
AciI CCGC 2 cut(s) 34, 267
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 3 cut(s) 103, 212, 568
AjnI CCWGG 1 cut(s) 744
AleI CACNNNNGTG 1 cut(s) 697
AluBI AGCT 4 cut(s) 310, 544, 730, 764
AluI AGCT 4 cut(s) 310, 544, 730, 764
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 2 cut(s) 529, 712
Ama87I CYCGRG 1 cut(s) 13
Aor13HI TCCGGA 1 cut(s) 546
AoxI GGCC 2 cut(s) 264, 737
ApeKI GCWGC 3 cut(s) 146, 202, 541
AseI ATTAAT 1 cut(s) 770
Asp700I GAANNNNTTC 1 cut(s) 559
AspLEI GCGC 2 cut(s) 408, 480
AspS9I GGNCC 4 cut(s) 264, 415, 660, 737
AsuC2I CCSGG 3 cut(s) 14, 15, 47
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 2 cut(s) 415, 660
BbsI GAAGAC 2 cut(s) 161, 642
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 3 cut(s) 133, 214, 553
BccI CCATC 2 cut(s) 209, 454
BciT130I CCWGG 1 cut(s) 746
BciVI GTATCC 1 cut(s) 334
BcnI CCSGG 3 cut(s) 14, 15, 47
BcoDI GTCTC 2 cut(s) 529, 712
BfaI CTAG 3 cut(s) 294, 371, 731
BfoI RGCGCY 1 cut(s) 481
BfuAI ACCTGC 1 cut(s) 632
BfuI GTATCC 1 cut(s) 334
BisI GCNGC 4 cut(s) 147, 203, 267, 542
BlsI GCNGC 4 cut(s) 148, 204, 268, 543
Bme1390I CCNGG 4 cut(s) 14, 15, 47, 746
Bme18I GGWCC 2 cut(s) 415, 660
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 4 cut(s) 264, 415, 660, 737
BmrFI CCNGG 4 cut(s) 14, 15, 47, 746
BmsI GCATC 3 cut(s) 13, 214, 274
BpiI GAAGAC 2 cut(s) 161, 642
BplI GAGNNNNNCTC 2 cut(s) 328, 360
BpuMI CCSGG 3 cut(s) 14, 15, 47
BsaJI CCNNGG 2 cut(s) 13, 744
BsaWI WCCGGW 1 cut(s) 546
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse3DI GCAATG 1 cut(s) 40
BseAI TCCGGA 1 cut(s) 546
BseBI CCWGG 1 cut(s) 746
BseDI CCNNGG 2 cut(s) 13, 744
BseGI GGATG 4 cut(s) 289, 465, 523, 535
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 1 cut(s) 359
BseRI GAGGAG 4 cut(s) 67, 181, 190, 265
BseXI GCAGC 3 cut(s) 133, 214, 553
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 266, 739
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 3 cut(s) 14, 46, 547
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 273
BsmAI GTCTC 2 cut(s) 529, 712
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 2 cut(s) 266, 739
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 439
Bsp13I TCCGGA 1 cut(s) 546
Bsp143I GATC 2 cut(s) 487, 789
BspACI CCGC 2 cut(s) 34, 267
BspANI GGCC 2 cut(s) 266, 739
BspCNI CTCAG 1 cut(s) 358
BspEI TCCGGA 1 cut(s) 546
BspFNI CGCG 1 cut(s) 28
BspMI ACCTGC 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 13, 744
BssMI GATC 2 cut(s) 487, 789
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 1 cut(s) 576
BstC8I GCNNGC 3 cut(s) 312, 386, 435
BstDEI CTNAG 1 cut(s) 345
BstF5I GGATG 4 cut(s) 289, 465, 523, 535
BstFNI CGCG 1 cut(s) 28
BstH2I RGCGCY 1 cut(s) 481
BstHHI GCGC 2 cut(s) 408, 480
BstKTI GATC 2 cut(s) 490, 792
BstMAI GTCTC 2 cut(s) 529, 712
BstMBI GATC 2 cut(s) 487, 789
BstMWI GCNNNNNNNGC 2 cut(s) 381, 736
BstNI CCWGG 1 cut(s) 746
BstSCI CCNGG 4 cut(s) 12, 13, 45, 744
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 3 cut(s) 133, 214, 553
BstV2I GAAGAC 2 cut(s) 161, 642
BstXI CCANNNNNNTGG 1 cut(s) 595
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 2 cut(s) 266, 739
BtsCI GGATG 4 cut(s) 289, 465, 523, 535
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 3 cut(s) 312, 386, 435
CfoI GCGC 2 cut(s) 408, 480
Cfr13I GGNCC 4 cut(s) 264, 415, 660, 737
Cfr9I CCCGGG 1 cut(s) 13
CseI GACGC 2 cut(s) 17, 647
CviAII CATG 1 cut(s) 631
DdeI CTNAG 1 cut(s) 345
DpnI GATC 2 cut(s) 489, 791
DpnII GATC 2 cut(s) 487, 789
DraI TTTAAA 1 cut(s) 423
Eco47I GGWCC 2 cut(s) 415, 660
Eco88I CYCGRG 1 cut(s) 13
EcoO109I RGGNCCY 2 cut(s) 415, 737
EcoRII CCWGG 1 cut(s) 744
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 1 cut(s) 634
FaiI YATR 9 cut(s) 96, 316, 507, 590, 628, 632, 650, 697, 707
FaqI GGGAC 1 cut(s) 30
FatI CATG 1 cut(s) 630
Fnu4HI GCNGC 4 cut(s) 147, 203, 267, 542
FokI GGATG 4 cut(s) 296, 472, 530, 542
Fsp4HI GCNGC 4 cut(s) 147, 203, 267, 542
FspBI CTAG 3 cut(s) 294, 371, 731
GlaI GCGC 2 cut(s) 407, 479
GluI GCNGC 4 cut(s) 147, 203, 267, 542
HaeII RGCGCY 1 cut(s) 481
HaeIII GGCC 2 cut(s) 266, 739
HapII CCGG 3 cut(s) 14, 46, 547
HgaI GACGC 2 cut(s) 17, 647
HhaI GCGC 2 cut(s) 408, 480
Hin1II CATG 1 cut(s) 634
Hin6I GCGC 2 cut(s) 406, 478
HinP1I GCGC 2 cut(s) 406, 478
HinfI GANTC 4 cut(s) 77, 304, 320, 353
HpaII CCGG 3 cut(s) 14, 46, 547
Hpy166II GTNNAC 2 cut(s) 257, 598
Hpy188I TCNGA 6 cut(s) 82, 112, 165, 348, 358, 492
Hpy188III TCNNGA 5 cut(s) 209, 324, 457, 547, 568
Hpy8I GTNNAC 2 cut(s) 257, 598
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 6 cut(s) 179, 229, 273, 358, 728, 769
HpyCH4III ACNGT 1 cut(s) 576
HpyCH4V TGCA 4 cut(s) 4, 149, 287, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 381, 736
HpyF3I CTNAG 1 cut(s) 345
Hsp92II CATG 1 cut(s) 634
HspAI GCGC 2 cut(s) 406, 478
Kpn2I TCCGGA 1 cut(s) 546
Kzo9I GATC 2 cut(s) 487, 789
LmnI GCTCC 4 cut(s) 238, 475, 549, 761
Lsp1109I GCAGC 3 cut(s) 133, 214, 553
LweI GCATC 3 cut(s) 13, 214, 274
MaeI CTAG 3 cut(s) 294, 371, 731
MalI GATC 2 cut(s) 489, 791
MboI GATC 2 cut(s) 487, 789
MboII GAAGA 3 cut(s) 166, 224, 647
MfeI CAATTG 1 cut(s) 750
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 6 cut(s) 89, 159, 297, 464, 606, 750
MlyI GAGTC 1 cut(s) 86
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 559
MseI TTAA 3 cut(s) 422, 611, 770
MslI CAYNNNNRTG 3 cut(s) 458, 593, 697
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 3 cut(s) 14, 46, 547
MspR9I CCNGG 4 cut(s) 14, 15, 47, 746
MunI CAATTG 1 cut(s) 750
Mva1269I GAATGC 1 cut(s) 403
MvaI CCWGG 1 cut(s) 746
MvnI CGCG 1 cut(s) 28
MwoI GCNNNNNNNGC 2 cut(s) 381, 736
NciI CCSGG 3 cut(s) 14, 15, 47
NdeII GATC 2 cut(s) 487, 789
NlaIII CATG 1 cut(s) 634
NsiI ATGCAT 1 cut(s) 6
OliI CACNNNNGTG 1 cut(s) 697
PaqCI CACCTGC 1 cut(s) 632
PctI GAATGC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 559
PfeI GAWTC 3 cut(s) 304, 320, 353
PkrI GCNGC 4 cut(s) 148, 204, 268, 543
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PpuMI RGGWCCY 1 cut(s) 415
PshBI ATTAAT 1 cut(s) 770
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 744
PspGI CCWGG 1 cut(s) 744
PspPI GGNCC 4 cut(s) 264, 415, 660, 737
PspPPI RGGWCCY 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 310
RseI CAYNNNNRTG 3 cut(s) 458, 593, 697
SaqAI TTAA 3 cut(s) 422, 611, 770
SatI GCNGC 4 cut(s) 147, 203, 267, 542
Sau3AI GATC 2 cut(s) 487, 789
Sau96I GGNCC 4 cut(s) 264, 415, 660, 737
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 4 cut(s) 14, 15, 47, 746
SduI GDGCHC 1 cut(s) 439
SetI ASST 9 cut(s) 312, 420, 525, 546, 646, 665, 681, 732, 766
SfaNI GCATC 3 cut(s) 13, 214, 274
SinI GGWCC 2 cut(s) 415, 660
SmaI CCCGGG 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 458, 593, 697
Sse9I AATT 6 cut(s) 89, 159, 297, 464, 606, 750
SsiI CCGC 2 cut(s) 34, 267
SspMI CTAG 3 cut(s) 294, 371, 731
StyD4I CCNGG 4 cut(s) 12, 13, 45, 744
TaaI ACNGT 1 cut(s) 576
TasI AATT 6 cut(s) 89, 159, 297, 464, 606, 750
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 3 cut(s) 304, 320, 353
Tru1I TTAA 3 cut(s) 422, 611, 770
Tru9I TTAA 3 cut(s) 422, 611, 770
TseI GCWGC 3 cut(s) 146, 202, 541
TspDTI ATGAA 2 cut(s) 410, 788
TspGWI ACGGA 3 cut(s) 349, 542, 615
TspMI CCCGGG 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 415, 660
VspI ATTAAT 1 cut(s) 770
XmaI CCCGGG 1 cut(s) 13
XmnI GAANNNNTTC 1 cut(s) 559
XspI CTAG 3 cut(s) 294, 371, 731
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.