Rroxscaffold_5G00332890

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012691
Physical Location & Seq
Forward (+)
258185 .. 262825
4641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00332890.1

Sequence Viewer

Length: 1041 bp
ATGGTGCTTTTTTGCCATCTCAAATTTCTGGTGGAGTTGGAGGAGTCCTACGAGATGCCAATGAACACCGCCGCCCTCTCCTCGGGCTCCCACGCCTTCAACGCCGAGAAGCTCCAGAACGCCATCGGCCTCCTCGAGTCCCGGCTCCGCTCCGAGGTCCTCTCTTGCCACTCCGACCTCCTCTCCCGGCTCTCCTCCCTCCGGCACGCCGACCACGCCCTCTCCACCCTCTCCAACCTCCACGCCACCTCCGAGCTCCTCCACCACACCCTCCGCGCCCTCCGCCTCTCCAAGAAGCTCCGCGACCTCGCCGCCGACCCCGAGAAGATCGATCTCGCCAAGGCCACCCAGTGCATTCCCCGTATCCACGGCTATCATCCAGTTCTAGATAAGGGCTTCGGGTATGTGCATTATGAAACTTTTGACACCCGCTTAATCCATGAATTACTGTCTCTTATGTTGCTCGATCTCTCCGTCATCGAAGTCGTCGAGGAGGAGCTCGGCCGGGTCGGAGAGACCGGCGACAAGTTGCGCGGCGAGGCCATGAAGGTTCGGATCATTGCTGCTCAAGCTCTCACAACTATGGCAATAAGATCTGGTGAGCCTTTTAGGCTACAAATCTATGAATTTTTACACACTATAGCAGAGGGTGGAGTGCAGTCTCAGTTTTCTGATATGCATCCCAGTAATGGAGAAGACCAGGGAGCTAATGGCACAGGCCTCGGAGTTCTAATCAGTCCCATGATAGAGGTTCTTGATGAAATGTATAGGGCACAAGATGATTTGATCAAGGAAATGCGCAACCATGATAATGCTAATAAGGAATGGACAGATGAAGAGCTTAAGAAACTCTATGAAACCCATGAAAGGTTGCTTGATCTTGTTTCTCTGTTTTGTTATGTTCCAAGAGCAAAATATCTGCCTTTGGGGCCAATAAGAGTAAGATACTGGCAAAGCCCTTTGGGTCTCCTTGATGTGAATGAAAACACAAATACAGCAACTAAGTGTGGAAGTGAACACTTAGAGCTAGGCCTGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

346

Amino Acids

38.92

Weight (kDa)

5.57

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 800
AccBSI CCGCTC 1 cut(s) 150
AccII CGCG 3 cut(s) 276, 303, 534
AciI CCGC 9 cut(s) 69, 72, 148, 274, 283, 301, 312, 430, 534
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 2 cut(s) 23, 626
AdeI CACNNNGTG 1 cut(s) 351
AfiI CCNNNNNNNGG 5 cut(s) 82, 154, 201, 689, 867
AflII CTTAAG 1 cut(s) 842
AgsI TTSAA 1 cut(s) 100
AjnI CCWGG 2 cut(s) 699, 1032
AluBI AGCT 8 cut(s) 112, 256, 298, 499, 572, 707, 841, 1027
AluI AGCT 8 cut(s) 112, 256, 298, 499, 572, 707, 841, 1027
Alw21I GWGCWC 2 cut(s) 258, 501
Alw26I GTCTC 4 cut(s) 456, 509, 666, 971
AlwI GGATC 1 cut(s) 563
Ama87I CYCGRG 3 cut(s) 82, 134, 320
AoxI GGCC 7 cut(s) 127, 342, 502, 540, 718, 929, 1030
ApeKI GCWGC 1 cut(s) 563
ApoI RAATTY 2 cut(s) 23, 626
AspLEI GCGC 3 cut(s) 278, 534, 801
AspS9I GGNCC 2 cut(s) 157, 929
AsuC2I CCSGG 3 cut(s) 142, 187, 506
AsuHPI GGTGA 1 cut(s) 611
AvaI CYCGRG 3 cut(s) 82, 134, 320
AvaII GGWCC 1 cut(s) 157
BaeGI GKGCMC 1 cut(s) 775
BanII GRGCYC 3 cut(s) 89, 258, 501
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 2 cut(s) 258, 501
BbvI GCAGC 1 cut(s) 550
BccI CCATC 2 cut(s) 24, 131
BceAI ACGGC 1 cut(s) 385
BcgI CGANNNNNNTGC 2 cut(s) 703, 737
BciT130I CCWGG 2 cut(s) 701, 1034
BciVI GTATCC 1 cut(s) 374
BclI TGATCA 1 cut(s) 786
BcnI CCSGG 3 cut(s) 142, 187, 506
BcoDI GTCTC 4 cut(s) 456, 509, 666, 971
BfaI CTAG 2 cut(s) 386, 1028
BfmI CTRYAG 1 cut(s) 639
BfrI CTTAAG 1 cut(s) 842
BfuI GTATCC 1 cut(s) 374
BglI GCCNNNNNGGC 2 cut(s) 610, 928
BglII AGATCT 1 cut(s) 593
BisI GCNGC 4 cut(s) 72, 312, 535, 564
BlsI GCNGC 4 cut(s) 73, 313, 536, 565
Bme1390I CCNGG 5 cut(s) 142, 187, 506, 701, 1034
Bme18I GGWCC 1 cut(s) 157
BmeT110I CYCGRG 3 cut(s) 82, 134, 320
BmgT120I GGNCC 2 cut(s) 157, 929
BmiI GGNNCC 3 cut(s) 88, 146, 930
BmrFI CCNGG 5 cut(s) 142, 187, 506, 701, 1034
BmrI ACTGGG 2 cut(s) 343, 678
BmsI GCATC 2 cut(s) 45, 688
BmuI ACTGGG 2 cut(s) 343, 678
BpiI GAAGAC 1 cut(s) 702
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BpmI CTGGAG 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 552
BpuMI CCSGG 3 cut(s) 142, 187, 506
Bsa29I ATCGAT 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 678
BsaI GGTCTC 2 cut(s) 509, 971
BsaJI CCNNGG 7 cut(s) 81, 153, 339, 367, 700, 721, 1033
BsaXI ACNNNNNCTCC 6 cut(s) 167, 197, 206, 233, 236, 263
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 154, 201, 689, 867
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 4 cut(s) 349, 380, 684, 953
Bse3DI GCAATG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 678
BseBI CCWGG 2 cut(s) 701, 1034
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 7 cut(s) 81, 153, 339, 367, 700, 721, 1033
BseGI GGATG 2 cut(s) 376, 679
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 5 cut(s) 82, 154, 201, 689, 867
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 1 cut(s) 677
BseNI ACTGG 4 cut(s) 349, 380, 684, 953
BseRI GAGGAG 8 cut(s) 56, 70, 122, 170, 184, 248, 506, 509
BseSI GKGCMC 1 cut(s) 775
BseX3I CGGCCG 1 cut(s) 502
BseXI GCAGC 1 cut(s) 550
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 3 cut(s) 276, 303, 534
Bsh1285I CGRYCG 1 cut(s) 505
BshFI GGCC 7 cut(s) 129, 344, 504, 542, 720, 931, 1032
BshVI ATCGAT 1 cut(s) 330
BsiEI CGRYCG 1 cut(s) 505
BsiHKAI GWGCWC 2 cut(s) 258, 501
BsiHKCI CYCGRG 3 cut(s) 82, 134, 320
BsiSI CCGG 5 cut(s) 142, 187, 202, 505, 519
BslFI GGGAC 2 cut(s) 124, 723
BslI CCNNNNNNNGG 5 cut(s) 82, 154, 201, 689, 867
BsmAI GTCTC 4 cut(s) 456, 509, 666, 971
BsmFI GGGAC 2 cut(s) 124, 723
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 7 cut(s) 129, 344, 504, 542, 720, 931, 1032
Bso31I GGTCTC 2 cut(s) 509, 971
BsoBI CYCGRG 3 cut(s) 82, 134, 320
Bsp1286I GDGCHC 4 cut(s) 89, 258, 501, 775
Bsp143I GATC 7 cut(s) 327, 331, 466, 555, 593, 786, 877
BspACI CCGC 9 cut(s) 69, 72, 148, 274, 283, 301, 312, 430, 534
BspANI GGCC 7 cut(s) 129, 344, 504, 542, 720, 931, 1032
BspCNI CTCAG 1 cut(s) 676
BspDI ATCGAT 1 cut(s) 330
BspFNI CGCG 3 cut(s) 276, 303, 534
BspLI GGNNCC 3 cut(s) 88, 146, 930
BspPI GGATC 1 cut(s) 563
BspQI GCTCTTC 1 cut(s) 831
BspTI CTTAAG 1 cut(s) 842
BspTNI GGTCTC 2 cut(s) 509, 971
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 4 cut(s) 349, 380, 684, 953
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 7 cut(s) 81, 153, 339, 367, 700, 721, 1033
BssMI GATC 7 cut(s) 327, 331, 466, 555, 593, 786, 877
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 2 cut(s) 701, 1034
Bst4CI ACNGT 1 cut(s) 450
Bst6I CTCTTC 1 cut(s) 831
BstAFI CTTAAG 1 cut(s) 842
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 3 cut(s) 663, 1002, 1021
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 2 cut(s) 376, 679
BstFNI CGCG 3 cut(s) 276, 303, 534
BstHHI GCGC 3 cut(s) 278, 534, 801
BstKTI GATC 7 cut(s) 330, 334, 469, 558, 596, 789, 880
BstMAI GTCTC 4 cut(s) 456, 509, 666, 971
BstMBI GATC 7 cut(s) 327, 331, 466, 555, 593, 786, 877
BstMCI CGRYCG 1 cut(s) 505
BstMWI GCNNNNNNNGC 6 cut(s) 101, 215, 282, 569, 610, 928
BstNI CCWGG 2 cut(s) 701, 1034
BstSCI CCNGG 5 cut(s) 140, 185, 504, 699, 1032
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 775
BstUI CGCG 3 cut(s) 276, 303, 534
BstV1I GCAGC 1 cut(s) 550
BstV2I GAAGAC 1 cut(s) 702
BstX2I RGATCY 1 cut(s) 593
BstYI RGATCY 1 cut(s) 593
BstZI CGGCCG 1 cut(s) 502
Bsu15I ATCGAT 1 cut(s) 330
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 7 cut(s) 129, 344, 504, 542, 720, 931, 1032
BsuTUI ATCGAT 1 cut(s) 330
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 2 cut(s) 376, 679
BtsIMutI CAGTG 1 cut(s) 356
Cac8I GCNNGC 1 cut(s) 207
CfoI GCGC 3 cut(s) 278, 534, 801
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 2 cut(s) 157, 929
ClaI ATCGAT 1 cut(s) 330
CviAII CATG 5 cut(s) 440, 544, 742, 806, 863
DdeI CTNAG 3 cut(s) 663, 1002, 1021
DpnI GATC 7 cut(s) 329, 333, 468, 557, 595, 788, 879
DpnII GATC 7 cut(s) 327, 331, 466, 555, 593, 786, 877
DraIII CACNNNGTG 1 cut(s) 351
EaeI YGGCCR 1 cut(s) 502
EagI CGGCCG 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
EciI GGCGGA 1 cut(s) 272
Ecl136II GAGCTC 2 cut(s) 256, 499
EclXI CGGCCG 1 cut(s) 502
Eco130I CCWWGG 1 cut(s) 339
Eco147I AGGCCT 2 cut(s) 720, 1032
Eco24I GRGCYC 3 cut(s) 89, 258, 501
Eco31I GGTCTC 2 cut(s) 509, 971
Eco47I GGWCC 1 cut(s) 157
Eco52I CGGCCG 1 cut(s) 502
Eco53kI GAGCTC 2 cut(s) 256, 499
Eco88I CYCGRG 3 cut(s) 82, 134, 320
EcoICRI GAGCTC 2 cut(s) 256, 499
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 699, 1032
EcoT14I CCWWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 681
EcoT38I GRGCYC 3 cut(s) 89, 258, 501
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 5 cut(s) 443, 547, 745, 809, 866
FaqI GGGAC 2 cut(s) 124, 723
FatI CATG 5 cut(s) 439, 543, 741, 805, 862
FauI CCCGC 1 cut(s) 437
FbaI TGATCA 1 cut(s) 786
Fnu4HI GCNGC 4 cut(s) 72, 312, 535, 564
FokI GGATG 2 cut(s) 363, 666
FriOI GRGCYC 3 cut(s) 89, 258, 501
Fsp4HI GCNGC 4 cut(s) 72, 312, 535, 564
FspBI CTAG 2 cut(s) 386, 1028
FspI TGCGCA 1 cut(s) 800
GlaI GCGC 3 cut(s) 277, 533, 800
GluI GCNGC 4 cut(s) 72, 312, 535, 564
GsuI CTGGAG 1 cut(s) 98
HaeIII GGCC 7 cut(s) 129, 344, 504, 542, 720, 931, 1032
HapII CCGG 5 cut(s) 142, 187, 202, 505, 519
HhaI GCGC 3 cut(s) 278, 534, 801
Hin1II CATG 5 cut(s) 443, 547, 745, 809, 866
Hin6I GCGC 3 cut(s) 276, 532, 799
HinP1I GCGC 3 cut(s) 276, 532, 799
HinfI GANTC 2 cut(s) 44, 137
HpaII CCGG 5 cut(s) 142, 187, 202, 505, 519
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 1 cut(s) 1016
Hpy188I TCNGA 7 cut(s) 154, 175, 253, 512, 555, 673, 725
Hpy188III TCNNGA 3 cut(s) 115, 386, 755
Hpy8I GTNNAC 1 cut(s) 1016
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 2 cut(s) 106, 541
HpyCH4III ACNGT 1 cut(s) 450
HpyCH4V TGCA 4 cut(s) 354, 409, 658, 679
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 215, 282, 569, 610, 928
HpyF3I CTNAG 3 cut(s) 663, 1002, 1021
Hsp92II CATG 5 cut(s) 443, 547, 745, 809, 866
HspAI GCGC 3 cut(s) 276, 532, 799
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 7 cut(s) 327, 331, 466, 555, 593, 786, 877
LguI GCTCTTC 1 cut(s) 831
LmnI GCTCC 8 cut(s) 92, 117, 150, 155, 261, 303, 496, 704
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 2 cut(s) 45, 688
MaeI CTAG 2 cut(s) 386, 1028
MalI GATC 7 cut(s) 329, 333, 468, 557, 595, 788, 879
MbiI CCGCTC 1 cut(s) 150
MboI GATC 7 cut(s) 327, 331, 466, 555, 593, 786, 877
MboII GAAGA 3 cut(s) 337, 707, 848
MflI RGATCY 1 cut(s) 593
MhlI GDGCHC 4 cut(s) 89, 258, 501, 775
MluCI AATT 3 cut(s) 23, 443, 626
MlyI GAGTC 2 cut(s) 53, 146
MmeI TCCRAC 4 cut(s) 18, 198, 258, 490
Mph1103I ATGCAT 1 cut(s) 681
MseI TTAA 2 cut(s) 434, 843
MslI CAYNNNNRTG 2 cut(s) 581, 810
MspCI CTTAAG 1 cut(s) 842
MspI CCGG 5 cut(s) 142, 187, 202, 505, 519
MspR9I CCNGG 5 cut(s) 142, 187, 506, 701, 1034
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 2 cut(s) 701, 1034
MvnI CGCG 3 cut(s) 276, 303, 534
MwoI GCNNNNNNNGC 6 cut(s) 101, 215, 282, 569, 610, 928
NciI CCSGG 3 cut(s) 142, 187, 506
NdeII GATC 7 cut(s) 327, 331, 466, 555, 593, 786, 877
NlaIII CATG 5 cut(s) 443, 547, 745, 809, 866
NlaIV GGNNCC 3 cut(s) 88, 146, 930
NmeAIII GCCGAG 2 cut(s) 130, 480
NsbI TGCGCA 1 cut(s) 800
NsiI ATGCAT 1 cut(s) 681
PaeR7I CTCGAG 1 cut(s) 134
PceI AGGCCT 2 cut(s) 720, 1032
PciSI GCTCTTC 1 cut(s) 831
PcsI WCGNNNNNNNCGW 4 cut(s) 132, 249, 471, 486
PctI GAATGC 1 cut(s) 354
PkrI GCNGC 4 cut(s) 73, 313, 536, 565
PleI GAGTC 2 cut(s) 52, 145
PpsI GAGTC 2 cut(s) 52, 145
PpuMI RGGWCCY 1 cut(s) 157
Psp124BI GAGCTC 2 cut(s) 258, 501
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 2 cut(s) 699, 1032
PspGI CCWGG 2 cut(s) 699, 1032
PspN4I GGNNCC 3 cut(s) 88, 146, 930
PspPI GGNCC 2 cut(s) 157, 929
PspPPI RGGWCCY 1 cut(s) 157
PspXI VCTCGAGB 1 cut(s) 134
PsuI RGATCY 1 cut(s) 593
RseI CAYNNNNRTG 2 cut(s) 581, 810
SacI GAGCTC 2 cut(s) 258, 501
SapI GCTCTTC 1 cut(s) 831
SaqAI TTAA 2 cut(s) 434, 843
SatI GCNGC 4 cut(s) 72, 312, 535, 564
Sau3AI GATC 7 cut(s) 327, 331, 466, 555, 593, 786, 877
Sau96I GGNCC 2 cut(s) 157, 929
SchI GAGTC 2 cut(s) 53, 146
ScrFI CCNGG 5 cut(s) 142, 187, 506, 701, 1034
SduI GDGCHC 4 cut(s) 89, 258, 501, 775
SfaNI GCATC 2 cut(s) 45, 688
SfcI CTRYAG 1 cut(s) 639
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 157
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 581, 810
SmlI CTYRAG 3 cut(s) 134, 567, 842
SmoI CTYRAG 3 cut(s) 134, 567, 842
Sse9I AATT 3 cut(s) 23, 443, 626
SseBI AGGCCT 2 cut(s) 720, 1032
SsiI CCGC 9 cut(s) 69, 72, 148, 274, 283, 301, 312, 430, 534
SspMI CTAG 2 cut(s) 386, 1028
SstI GAGCTC 2 cut(s) 258, 501
StuI AGGCCT 2 cut(s) 720, 1032
StyD4I CCNGG 5 cut(s) 140, 185, 504, 699, 1032
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 1 cut(s) 450
TaqI TCGA 5 cut(s) 135, 330, 465, 480, 489
TasI AATT 3 cut(s) 23, 443, 626
TauI GCSGC 3 cut(s) 74, 314, 537
Tru1I TTAA 2 cut(s) 434, 843
Tru9I TTAA 2 cut(s) 434, 843
TscAI CASTG 1 cut(s) 356
TseI GCWGC 1 cut(s) 563
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 1 cut(s) 356
Vha464I CTTAAG 1 cut(s) 842
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 2 cut(s) 23, 626
XbaI TCTAGA 1 cut(s) 385
XcmI CCANNNNNNNNNTGG 1 cut(s) 707
XhoI CTCGAG 1 cut(s) 134
XspI CTAG 2 cut(s) 386, 1028
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.