RchiOBHm_Chr4g0418951

Guanylate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0013311
Physical Location & Seq
Forward (+)
44457221 .. 44460013
2793 bp
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UTR
Exon/CDS
Intron
PRQ38889

Sequence Viewer

Length: 1071 bp
ATGCCATCATTATTCGTCAACAAATCAAAAGATATTGACACTGTTTTCCTTTCTCCTCAAATTCTTGGTGCCACCAAATCCCCAAAACGGTCCAAAACTCTCAAAACTTCTAGCGAAATCCCACACTCCACTCCACACCTAACCACAGGAGCCCAAATTCAATGCTCTGCTTCTCCAACCTCATATAGTGCACTCTCAATGTTTCGCAGATTCCTCACCACCTCTCTCTCCTCCCGCACCAACCCCCCTCCTCTCCTCTTCCTCCTCTCCCCCAAATCCCCCAAAACCCCCAATTCCCTCTCCCCCAATCCAAACCCTAACCCCCCATTCCCCCGATTCCTCTCCCAAATGTCCGACGCCCGCCGCCCCACCTCCGCCTCCATCCCCGCCCTCAACAAAGCCGACCGCTCCGACCTCCTCCGCGCCCTCGAGTCCTCCCTCGGCTCCGCCTTCACCTCCGAGCCCCTCTGCCCCGCCCCAGCCCCCCTCATCATCGTCATCAGCGGCCCCAGCGGCGTCGGCAAAGACGCCGTGATTAAAAAGCTCCGGGATGTCAACCCTAACCTCCATTTCGTCGTCACGGCGACCAGCCGGCCCATCCGGCCCGGCGAGGTCCACGGTAAAGACTATTACTTTGTGAGCAAGGAAGAGTTTTTAAGTATGGTGCATAAAGATGAGCTTCTGGAATATGCATTGGTGTATGGAGACTATAAGGGGATTCCGAAACAGCAGATTAGGGATTACATGGGGAAAGGGCATGACATTGTCTTGAGAGTTGATATTCAGGGGGCTCAGACTTTGAGGAGGATTTTGGGGGATTCTGCTGTGTTTATATTCTTGATGGCCGAGAGTGAGGCTAAGCTTGTGGAGAGGTTGGTGGATAGGAAGACCGAGACGAAGGAGTCTTTGCTTGTGAGGATTGCGACGGCGAGGGAGGAGGTGAAGCATGTCAAGAGTTTTGATTATGTGGTGGTGAATGCCGAGGGGAGGCTGGACAATGCGGTGCAGTTGGTGGAGTCGATTGTTCGCGCCGAGAAGGCTAAGGTGCACCAGAGGAGAAGTGTGATTTAG

Protein Analysis

356

Amino Acids

39.31

Weight (kDa)

9.71

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Guanylate_kin PF00625 165 - 344 4.9e-39 Guanylate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013311)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06200
fragaria_vesca FvH4_4g14220
malus_domestica MD13G1180900.v1.1 MD16G1181600.v1.1
prunus_persica Prupe.1G149000_v2.0.a1 Prupe.1G149000_v2.0.a1
pyrus_communis pycom13g15610 pycom16g15240
rosa_chinensis RchiOBHm_Chr4g0418951
rosa_laevigata RLG00000007839
rosa_multiflora Rmu_sc0000935.1_g000061
rosa_roxburghii Rroxscaffold_5G00362040
rosa_rugosa Rorug04G0155400 Rorug04G0155500
rosa_samantha Rh4BG212800 Rh4CG229400 Rh4DG216100
rosa_wichuraiana Rw4G018580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 901
AccB1I GGYRCC 1 cut(s) 68
AccBSI CCGCTC 1 cut(s) 408
AccII CGCG 2 cut(s) 423, 1029
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 2 cut(s) 60, 156
AcyI GRCGYC 3 cut(s) 357, 516, 528
AfiI CCNNNNNNNGG 2 cut(s) 87, 987
AgsI TTSAA 1 cut(s) 161
AluBI AGCT 3 cut(s) 544, 679, 862
AluI AGCT 3 cut(s) 544, 679, 862
Alw21I GWGCWC 2 cut(s) 193, 1050
Alw26I GTCTC 2 cut(s) 699, 887
Alw44I GTGCAC 2 cut(s) 189, 1046
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 4 cut(s) 505, 593, 602, 843
ApaLI GTGCAC 2 cut(s) 189, 1046
ApoI RAATTY 2 cut(s) 60, 156
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
AspLEI GCGC 2 cut(s) 425, 1031
AspS9I GGNCC 5 cut(s) 90, 506, 594, 603, 613
AsuC2I CCSGG 2 cut(s) 548, 606
AsuHPI GGTGA 4 cut(s) 208, 445, 952, 985
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 2 cut(s) 90, 613
BaeGI GKGCMC 2 cut(s) 193, 1050
BanI GGYRCC 1 cut(s) 68
BanII GRGCYC 3 cut(s) 154, 465, 793
BbsI GAAGAC 1 cut(s) 893
Bbv12I GWGCWC 2 cut(s) 193, 1050
BccI CCATC 4 cut(s) 13, 389, 605, 835
BceAI ACGGC 3 cut(s) 515, 597, 942
BcnI CCSGG 2 cut(s) 548, 606
BcoDI GTCTC 2 cut(s) 699, 887
BfaI CTAG 1 cut(s) 111
BglI GCCNNNNNGGC 3 cut(s) 513, 601, 1037
BisI GCNGC 3 cut(s) 364, 505, 514
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 3 cut(s) 365, 506, 515
Bme1390I CCNGG 2 cut(s) 548, 606
Bme18I GGWCC 2 cut(s) 90, 613
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 5 cut(s) 90, 506, 594, 603, 613
BmiI GGNNCC 4 cut(s) 70, 151, 445, 508
BmrFI CCNGG 2 cut(s) 548, 606
BpiI GAAGAC 1 cut(s) 893
Bpu10I CCTNAGC 1 cut(s) 1041
Bpu1102I GCTNAGC 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 790
BpuMI CCSGG 2 cut(s) 548, 606
BsaHI GRCGYC 3 cut(s) 357, 516, 528
BsaJI CCNNGG 3 cut(s) 439, 616, 981
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 987
Bse118I RCCGGY 1 cut(s) 591
BseDI CCNNGG 3 cut(s) 439, 616, 981
BseGI GGATG 3 cut(s) 381, 556, 597
BseLI CCNNNNNNNGG 2 cut(s) 87, 987
BseMII CTCAG 1 cut(s) 806
BseRI GAGGAG 9 cut(s) 45, 220, 240, 245, 254, 407, 817, 950, 1069
BseSI GKGCMC 2 cut(s) 193, 1050
BseYI CCCAGC 2 cut(s) 478, 509
BsgI GTGCAG 1 cut(s) 1025
Bsh1236I CGCG 2 cut(s) 423, 1029
Bsh1285I CGRYCG 1 cut(s) 406
BshFI GGCC 4 cut(s) 507, 595, 604, 845
BshNI GGYRCC 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 406
BsiHKAI GWGCWC 2 cut(s) 193, 1050
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 4 cut(s) 547, 592, 601, 606
BslI CCNNNNNNNGG 2 cut(s) 87, 987
BsmAI GTCTC 2 cut(s) 699, 887
BsmBI CGTCTC 1 cut(s) 887
BsmI GAATGC 1 cut(s) 982
BsnI GGCC 4 cut(s) 507, 595, 604, 845
BsoBI CYCGRG 1 cut(s) 428
Bsp1286I GDGCHC 5 cut(s) 154, 193, 465, 793, 1050
Bsp1720I GCTNAGC 1 cut(s) 858
BspANI GGCC 4 cut(s) 507, 595, 604, 845
BspCNI CTCAG 1 cut(s) 805
BspFNI CGCG 2 cut(s) 423, 1029
BspLI GGNNCC 4 cut(s) 70, 151, 445, 508
BspT107I GGYRCC 1 cut(s) 68
BsrBI CCGCTC 1 cut(s) 408
BsrFI RCCGGY 1 cut(s) 591
BssAI RCCGGY 1 cut(s) 591
BssECI CCNNGG 3 cut(s) 439, 616, 981
BssNI GRCGYC 3 cut(s) 357, 516, 528
Bst4CI ACNGT 3 cut(s) 43, 90, 620
Bst6I CTCTTC 2 cut(s) 263, 642
BstACI GRCGYC 3 cut(s) 357, 516, 528
BstC8I GCNNGC 2 cut(s) 361, 593
BstDEI CTNAG 3 cut(s) 792, 858, 1041
BstDSI CCRYGG 1 cut(s) 616
BstF5I GGATG 3 cut(s) 381, 556, 597
BstFNI CGCG 2 cut(s) 423, 1029
BstHHI GCGC 2 cut(s) 425, 1031
BstMAI GTCTC 2 cut(s) 699, 887
BstMCI CGRYCG 1 cut(s) 406
BstMWI GCNNNNNNNGC 5 cut(s) 510, 513, 519, 601, 1037
BstNSI RCATGY 1 cut(s) 950
BstSCI CCNGG 2 cut(s) 546, 604
BstSLI GKGCMC 2 cut(s) 193, 1050
BstUI CGCG 2 cut(s) 423, 1029
BstV2I GAAGAC 1 cut(s) 893
BsuRI GGCC 4 cut(s) 507, 595, 604, 845
BtgI CCRYGG 1 cut(s) 616
BtsCI GGATG 3 cut(s) 381, 556, 597
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 361, 593
CfoI GCGC 2 cut(s) 425, 1031
Cfr10I RCCGGY 1 cut(s) 591
Cfr13I GGNCC 5 cut(s) 90, 506, 594, 603, 613
CseI GACGC 3 cut(s) 365, 505, 536
CviAII CATG 3 cut(s) 745, 758, 947
DdeI CTNAG 3 cut(s) 792, 858, 1041
DrdI GACNNNNNNGTC 1 cut(s) 901
DseDI GACNNNNNNGTC 1 cut(s) 901
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 2 cut(s) 263, 642
EarI CTCTTC 2 cut(s) 263, 642
EciI GGCGGA 2 cut(s) 364, 436
Eco24I GRGCYC 3 cut(s) 154, 465, 793
Eco47I GGWCC 2 cut(s) 90, 613
Eco88I CYCGRG 1 cut(s) 428
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 3 cut(s) 154, 465, 793
Esp3I CGTCTC 1 cut(s) 887
FaeI CATG 3 cut(s) 748, 761, 950
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FatI CATG 3 cut(s) 744, 757, 946
FauI CCCGC 4 cut(s) 242, 368, 394, 481
Fnu4HI GCNGC 3 cut(s) 364, 505, 514
FokI GGATG 3 cut(s) 368, 563, 584
FriOI GRGCYC 3 cut(s) 154, 465, 793
Fsp4HI GCNGC 3 cut(s) 364, 505, 514
FspBI CTAG 1 cut(s) 111
GlaI GCGC 2 cut(s) 424, 1030
GluI GCNGC 3 cut(s) 364, 505, 514
GsaI CCCAGC 2 cut(s) 482, 513
HaeIII GGCC 4 cut(s) 507, 595, 604, 845
HapII CCGG 4 cut(s) 547, 592, 601, 606
HgaI GACGC 3 cut(s) 365, 505, 536
HhaI GCGC 2 cut(s) 425, 1031
Hin1I GRCGYC 3 cut(s) 357, 516, 528
Hin1II CATG 3 cut(s) 748, 761, 950
Hin6I GCGC 2 cut(s) 423, 1029
HinP1I GCGC 2 cut(s) 423, 1029
HincII GTYRAC 2 cut(s) 19, 556
HindII GTYRAC 2 cut(s) 19, 556
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 7 cut(s) 210, 336, 431, 718, 818, 902, 1016
HpaII CCGG 4 cut(s) 547, 592, 601, 606
HphI GGTGA 4 cut(s) 208, 445, 952, 985
Hpy166II GTNNAC 5 cut(s) 19, 191, 556, 616, 1048
Hpy188I TCNGA 5 cut(s) 355, 412, 460, 723, 795
Hpy188III TCNNGA 4 cut(s) 683, 769, 838, 952
Hpy8I GTNNAC 5 cut(s) 19, 191, 556, 616, 1048
Hpy99I CGWCG 4 cut(s) 359, 521, 578, 928
HpyAV CCTTC 3 cut(s) 460, 892, 1030
HpyCH4III ACNGT 3 cut(s) 43, 90, 620
HpyCH4V TGCA 5 cut(s) 191, 667, 692, 1006, 1048
HpyF10VI GCNNNNNNNGC 5 cut(s) 510, 513, 519, 601, 1037
HpyF3I CTNAG 3 cut(s) 792, 858, 1041
Hsp92I GRCGYC 3 cut(s) 357, 516, 528
Hsp92II CATG 3 cut(s) 748, 761, 950
HspAI GCGC 2 cut(s) 423, 1029
KroI GCCGGC 1 cut(s) 591
KroNI GCCGGC 1 cut(s) 593
LmnI GCTCC 4 cut(s) 149, 413, 449, 549
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 577
MbiI CCGCTC 1 cut(s) 408
MboII GAAGA 3 cut(s) 250, 659, 898
MhlI GDGCHC 5 cut(s) 154, 193, 465, 793, 1050
MluCI AATT 3 cut(s) 60, 156, 292
MlyI GAGTC 3 cut(s) 440, 911, 1025
MmeI TCCRAC 3 cut(s) 200, 378, 435
Mph1103I ATGCAT 1 cut(s) 694
MroNI GCCGGC 1 cut(s) 591
MseI TTAA 2 cut(s) 537, 656
MslI CAYNNNNRTG 1 cut(s) 672
MspA1I CMGCKG 2 cut(s) 504, 513
MspI CCGG 4 cut(s) 547, 592, 601, 606
MspR9I CCNGG 2 cut(s) 548, 606
Mva1269I GAATGC 1 cut(s) 982
MvnI CGCG 2 cut(s) 423, 1029
MwoI GCNNNNNNNGC 5 cut(s) 510, 513, 519, 601, 1037
NaeI GCCGGC 1 cut(s) 593
NciI CCSGG 2 cut(s) 548, 606
NgoMIV GCCGGC 1 cut(s) 591
NlaIII CATG 3 cut(s) 748, 761, 950
NlaIV GGNNCC 4 cut(s) 70, 151, 445, 508
NmeAIII GCCGAG 4 cut(s) 420, 871, 1006, 1057
NmuCI GTSAC 1 cut(s) 577
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 1 cut(s) 950
PaeR7I CTCGAG 1 cut(s) 428
PctI GAATGC 1 cut(s) 982
PdiI GCCGGC 1 cut(s) 593
PfeI GAWTC 4 cut(s) 210, 336, 718, 818
PflFI GACNNNGTC 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 546
PkrI GCNGC 3 cut(s) 365, 506, 515
PleI GAGTC 3 cut(s) 439, 910, 1024
PpsI GAGTC 3 cut(s) 439, 910, 1024
PspFI CCCAGC 2 cut(s) 478, 509
PspN4I GGNNCC 4 cut(s) 70, 151, 445, 508
PspPI GGNCC 5 cut(s) 90, 506, 594, 603, 613
PspXI VCTCGAGB 1 cut(s) 428
PsyI GACNNNGTC 1 cut(s) 764
RseI CAYNNNNRTG 1 cut(s) 672
SaqAI TTAA 2 cut(s) 537, 656
SatI GCNGC 3 cut(s) 364, 505, 514
Sau96I GGNCC 5 cut(s) 90, 506, 594, 603, 613
SchI GAGTC 3 cut(s) 440, 911, 1025
ScrFI CCNGG 2 cut(s) 548, 606
SduI GDGCHC 5 cut(s) 154, 193, 465, 793, 1050
SfiI GGCCNNNNNGGCC 1 cut(s) 601
Sfr274I CTCGAG 1 cut(s) 428
SinI GGWCC 2 cut(s) 90, 613
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 672
SmlI CTYRAG 2 cut(s) 428, 769
SmoI CTYRAG 2 cut(s) 428, 769
Sse9I AATT 3 cut(s) 60, 156, 292
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 546, 604
TaaI ACNGT 3 cut(s) 43, 90, 620
TaqI TCGA 2 cut(s) 429, 1019
TaqII GACCGA 1 cut(s) 905
TasI AATT 3 cut(s) 60, 156, 292
TauI GCSGC 3 cut(s) 366, 507, 516
TfiI GAWTC 4 cut(s) 210, 336, 718, 818
Tru1I TTAA 2 cut(s) 537, 656
Tru9I TTAA 2 cut(s) 537, 656
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 577
Tsp45I GTSAC 1 cut(s) 577
TspRI CASTG 1 cut(s) 46
Tth111I GACNNNGTC 1 cut(s) 764
VneI GTGCAC 2 cut(s) 189, 1046
VpaK11BI GGWCC 2 cut(s) 90, 613
XapI RAATTY 2 cut(s) 60, 156
XceI RCATGY 1 cut(s) 950
XhoI CTCGAG 1 cut(s) 428
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.