Rroxscaffold_5G00362040

Guanylate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013311
Physical Location & Seq
Forward (+)
42878542 .. 42879264
723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362040.1

Sequence Viewer

Length: 723 bp
ATGTCCGACGCCCGCCGCCCCACCTCCTCCCCCATCCCCGCCCTCGACAAAGCCGACCGCTCCGACCTCCTCCGCGCCCTCGAATCCTCCCTCGGCTCCGCCTTCACCTCCGAGCCCCTCTACCCCGCCCCTTCCCCCCTCATCATCGTCATCAGCGGCCCCAGCGGCGTCGGCAAAGACGCCGTGATAAAAAAGCTCCGGGATGTCAACCCTAACCTCCATTTCGTCGTCACGGCGACCAGCCGGCCCATCCGGCCCGGCGAGGTCCACGGTAAAGACTATTACTTTGTGAGCAAGGAAGAGTTTTTGAGTATGGTGCATAAAGATGAGCTTCTGGAATATGCATTGGTGTATGGGGATTATAAGGGGATTCCGAAACAGCAGATTAGGGATTACATGGGGAAAGGGCATGACATTGTGTTGAGAGTTGATATTCAGGGGGCTCAGACTTTGAGGAGAATTCTTGGGGATTCGGCTGTGTTTATTTTTTTGATGGCTGAGAGTGAGGCTAAGCTTGTGGAGAGGTTGGTGGATAGGAAGACCGAGACGAAGGAGTCTTTGCTTGTGAGGATTGCTACGGCGAGGGAGGAGGTGAAGCATGTCAAGAGTTTTGATTATGTGGTGGTGAATGCCGAGGGGAGGCTGGACAATGCGGTGCAGTTAGTGCAGTCGATTATTTGCGCCGAGAAGGCCAAGGTTCACCAGAGGAGAAGTGTGATTTAG

Protein Analysis

240

Amino Acids

26.75

Weight (kDa)

8.84

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Guanylate_kin PF00625 49 - 228 1.4e-39 Guanylate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013311)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06200
fragaria_vesca FvH4_4g14220
malus_domestica MD13G1180900.v1.1 MD16G1181600.v1.1
prunus_persica Prupe.1G149000_v2.0.a1 Prupe.1G149000_v2.0.a1
pyrus_communis pycom13g15610 pycom16g15240
rosa_chinensis RchiOBHm_Chr4g0418951
rosa_laevigata RLG00000007839
rosa_multiflora Rmu_sc0000935.1_g000061
rosa_roxburghii Rroxscaffold_5G00362040
rosa_rugosa Rorug04G0155400 Rorug04G0155500
rosa_samantha Rh4BG212800 Rh4CG229400 Rh4DG216100
rosa_wichuraiana Rw4G018580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 363
AasI GACNNNNNNGTC 1 cut(s) 553
AccBSI CCGCTC 1 cut(s) 60
AccII CGCG 1 cut(s) 75
AcsI RAATTY 1 cut(s) 459
AcyI GRCGYC 3 cut(s) 9, 168, 180
AfiI CCNNNNNNNGG 1 cut(s) 639
AluBI AGCT 3 cut(s) 196, 331, 514
AluI AGCT 3 cut(s) 196, 331, 514
Alw26I GTCTC 1 cut(s) 539
AoxI GGCC 4 cut(s) 157, 245, 254, 690
ApoI RAATTY 1 cut(s) 459
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AspLEI GCGC 2 cut(s) 77, 683
AspS9I GGNCC 4 cut(s) 158, 246, 255, 265
AsuC2I CCSGG 2 cut(s) 200, 258
AsuHPI GGTGA 4 cut(s) 97, 604, 637, 692
AvaII GGWCC 1 cut(s) 265
BanII GRGCYC 2 cut(s) 117, 445
BbsI GAAGAC 1 cut(s) 545
BccI CCATC 3 cut(s) 41, 257, 487
BceAI ACGGC 3 cut(s) 167, 249, 594
BcnI CCSGG 2 cut(s) 200, 258
BcoDI GTCTC 1 cut(s) 539
BglI GCCNNNNNGGC 3 cut(s) 165, 253, 689
BisI GCNGC 3 cut(s) 16, 157, 166
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 3 cut(s) 17, 158, 167
Bme1390I CCNGG 2 cut(s) 200, 258
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 4 cut(s) 158, 246, 255, 265
BmiI GGNNCC 2 cut(s) 97, 160
BmrFI CCNGG 2 cut(s) 200, 258
BpiI GAAGAC 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 510
BpuMI CCSGG 2 cut(s) 200, 258
BsaHI GRCGYC 3 cut(s) 9, 168, 180
BsaJI CCNNGG 4 cut(s) 91, 268, 633, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 639
Bse118I RCCGGY 1 cut(s) 243
BseDI CCNNGG 4 cut(s) 91, 268, 633, 693
BseGI GGATG 3 cut(s) 33, 208, 249
BseLI CCNNNNNNNGG 1 cut(s) 639
BseMII CTCAG 2 cut(s) 458, 489
BseRI GAGGAG 5 cut(s) 16, 59, 469, 602, 721
BseYI CCCAGC 1 cut(s) 161
BsgI GTGCAG 2 cut(s) 677, 686
Bsh1236I CGCG 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 4 cut(s) 159, 247, 256, 692
BsiEI CGRYCG 1 cut(s) 58
BsiSI CCGG 4 cut(s) 199, 244, 253, 258
BslI CCNNNNNNNGG 1 cut(s) 639
BsmAI GTCTC 1 cut(s) 539
BsmBI CGTCTC 1 cut(s) 539
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 4 cut(s) 159, 247, 256, 692
Bsp1286I GDGCHC 2 cut(s) 117, 445
Bsp1720I GCTNAGC 1 cut(s) 510
BspANI GGCC 4 cut(s) 159, 247, 256, 692
BspCNI CTCAG 2 cut(s) 457, 490
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 97, 160
BsrBI CCGCTC 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 243
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 4 cut(s) 91, 268, 633, 693
BssNI GRCGYC 3 cut(s) 9, 168, 180
BssT1I CCWWGG 1 cut(s) 693
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 294
BstACI GRCGYC 3 cut(s) 9, 168, 180
BstAPI GCANNNNNTGC 1 cut(s) 664
BstC8I GCNNGC 2 cut(s) 13, 245
BstDEI CTNAG 3 cut(s) 444, 498, 510
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 3 cut(s) 33, 208, 249
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 2 cut(s) 77, 683
BstMAI GTCTC 1 cut(s) 539
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 6 cut(s) 162, 165, 171, 253, 664, 689
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 2 cut(s) 198, 256
BstUI CGCG 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 545
BsuRI GGCC 4 cut(s) 159, 247, 256, 692
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 3 cut(s) 33, 208, 249
Cac8I GCNNGC 2 cut(s) 13, 245
CfoI GCGC 2 cut(s) 77, 683
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 4 cut(s) 158, 246, 255, 265
CseI GACGC 3 cut(s) 17, 157, 188
CviAII CATG 3 cut(s) 397, 410, 599
DdeI CTNAG 3 cut(s) 444, 498, 510
DrdI GACNNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 553
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 693
Eco24I GRGCYC 2 cut(s) 117, 445
Eco47I GGWCC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 459
EcoT14I CCWWGG 1 cut(s) 693
EcoT22I ATGCAT 1 cut(s) 346
EcoT38I GRGCYC 2 cut(s) 117, 445
ErhI CCWWGG 1 cut(s) 693
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 3 cut(s) 400, 413, 602
FaiI YATR 9 cut(s) 314, 321, 342, 354, 363, 398, 411, 600, 618
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FatI CATG 3 cut(s) 396, 409, 598
FauI CCCGC 3 cut(s) 20, 46, 133
Fnu4HI GCNGC 3 cut(s) 16, 157, 166
FokI GGATG 3 cut(s) 20, 215, 236
FriOI GRGCYC 2 cut(s) 117, 445
Fsp4HI GCNGC 3 cut(s) 16, 157, 166
GlaI GCGC 2 cut(s) 76, 682
GluI GCNGC 3 cut(s) 16, 157, 166
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 4 cut(s) 159, 247, 256, 692
HapII CCGG 4 cut(s) 199, 244, 253, 258
HgaI GACGC 3 cut(s) 17, 157, 188
HhaI GCGC 2 cut(s) 77, 683
Hin1I GRCGYC 3 cut(s) 9, 168, 180
Hin1II CATG 3 cut(s) 400, 413, 602
Hin6I GCGC 2 cut(s) 75, 681
HinP1I GCGC 2 cut(s) 75, 681
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 4 cut(s) 83, 370, 470, 554
HpaII CCGG 4 cut(s) 199, 244, 253, 258
HphI GGTGA 4 cut(s) 97, 604, 637, 692
Hpy166II GTNNAC 3 cut(s) 208, 268, 700
Hpy188I TCNGA 5 cut(s) 7, 64, 112, 375, 447
Hpy188III TCNNGA 2 cut(s) 335, 604
Hpy8I GTNNAC 3 cut(s) 208, 268, 700
Hpy99I CGWCG 3 cut(s) 11, 173, 230
HpyAV CCTTC 4 cut(s) 112, 141, 544, 682
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4V TGCA 4 cut(s) 319, 344, 658, 667
HpyF10VI GCNNNNNNNGC 6 cut(s) 162, 165, 171, 253, 664, 689
HpyF3I CTNAG 3 cut(s) 444, 498, 510
Hsp92I GRCGYC 3 cut(s) 9, 168, 180
Hsp92II CATG 3 cut(s) 400, 413, 602
HspAI GCGC 2 cut(s) 75, 681
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
LmnI GCTCC 3 cut(s) 65, 101, 201
MaeIII GTNAC 1 cut(s) 229
MbiI CCGCTC 1 cut(s) 60
MboII GAAGA 2 cut(s) 311, 550
MhlI GDGCHC 2 cut(s) 117, 445
MluCI AATT 1 cut(s) 459
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 2 cut(s) 30, 87
Mph1103I ATGCAT 1 cut(s) 346
MroNI GCCGGC 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 324
MspA1I CMGCKG 2 cut(s) 156, 165
MspI CCGG 4 cut(s) 199, 244, 253, 258
MspR9I CCNGG 2 cut(s) 200, 258
Mva1269I GAATGC 1 cut(s) 634
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 6 cut(s) 162, 165, 171, 253, 664, 689
NaeI GCCGGC 1 cut(s) 245
NciI CCSGG 2 cut(s) 200, 258
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 3 cut(s) 400, 413, 602
NlaIV GGNNCC 2 cut(s) 97, 160
NmeAIII GCCGAG 3 cut(s) 72, 658, 709
NmuCI GTSAC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 1 cut(s) 602
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 1 cut(s) 634
PdiI GCCGGC 1 cut(s) 245
PfeI GAWTC 3 cut(s) 83, 370, 470
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 3 cut(s) 17, 158, 167
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
PsiI TTATAA 1 cut(s) 363
PspFI CCCAGC 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 97, 160
PspPI GGNCC 4 cut(s) 158, 246, 255, 265
RseI CAYNNNNRTG 1 cut(s) 324
SatI GCNGC 3 cut(s) 16, 157, 166
Sau96I GGNCC 4 cut(s) 158, 246, 255, 265
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 2 cut(s) 200, 258
SduI GDGCHC 2 cut(s) 117, 445
SfiI GGCCNNNNNGGCC 1 cut(s) 253
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 324
Sse9I AATT 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 198, 256
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 1 cut(s) 272
TaqI TCGA 3 cut(s) 45, 81, 671
TaqII GACCGA 1 cut(s) 557
TasI AATT 1 cut(s) 459
TauI GCSGC 3 cut(s) 18, 159, 168
TfiI GAWTC 3 cut(s) 83, 370, 470
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 1 cut(s) 459
XceI RCATGY 1 cut(s) 602
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.