RLG00000007839

Guanylate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013311
Physical Location & Seq
Reverse (-)
21770507 .. 21772620
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007839

Sequence Viewer

Length: 813 bp
ATGTCCGACGCCCGCCGCCCCACCTCCGCCTCCATCCCCGCCCTCGACAAAGCCGACCGCTCCGACCTCCTCCGCGCCCTCGAATCCTCCCTCGGCTCCGCCTTCACCTCCGAGCCCCTCTGCCCCGCCCCTTCCCCCCTCATCATCGTCATCAGCGGCCCCAGCGGCGTCGGCAAAGACGCCGTGATAAAAAAGCTCCGGGATGTCAACCCTAACCTCCATTTCGTCGTCACGGCGACCAGCCGGCCCATCCGGCCCGGCGAGGTCGACGGTAAAGACTATTACTTTGTGAGCAAGGAAGAGTTTTTGAGTATGGCTCATAAAGATGAGCTTCTGGAATATGCATTGGTGTATGGGGACTATAAGGGGATTCCGAAACAGCAGATTAGGGATTACATGGGGAAAGGGCATGACATTGTGTTGAGAGTTGATATTCAGGGTGCTCAGACATTGAGGAGGATTTTGGGGGATTCTGCTGTGTTTATATTCTTGATGGCTGAGAGTGAGGCTAAGCTGGTGGAGAGGTTGGTGGATAGGAAGACCGAGACAAAGGAGTCTTTGCTTGCTGGACAATGCGGTGCAGTTAGTGCAGTCGATTATTTGCGCCGAGAAGGCCAAGGTTCACCAGAGGAGAAGTGTGATTTAGCTGCGGAGTTGGCAAGAGGTGGAAATTGTAATCCAGCTGCATATGTAACTTGTCGCGTGATTAAAGTGTGTTGGGGCAAGCAACACACCGGTGGTGATGGAAGCATGATTATCAGCCGCCTGCTTCAAAATTTTGTCTGCAAGTTCTGCACTCAAGATATAAAGTAG

Protein Analysis

271

Amino Acids

29.54

Weight (kDa)

7.53

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Guanylate_kin PF00625 49 - 188 7.9e-31 Guanylate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013311)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06200
fragaria_vesca FvH4_4g14220
malus_domestica MD13G1180900.v1.1 MD16G1181600.v1.1
prunus_persica Prupe.1G149000_v2.0.a1 Prupe.1G149000_v2.0.a1
pyrus_communis pycom13g15610 pycom16g15240
rosa_chinensis RchiOBHm_Chr4g0418951
rosa_laevigata RLG00000007839
rosa_multiflora Rmu_sc0000935.1_g000061
rosa_roxburghii Rroxscaffold_5G00362040
rosa_rugosa Rorug04G0155400 Rorug04G0155500
rosa_samantha Rh4BG212800 Rh4CG229400 Rh4DG216100
rosa_wichuraiana Rw4G018580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 553
AccBSI CCGCTC 1 cut(s) 60
AccI GTMKAC 1 cut(s) 267
AccII CGCG 2 cut(s) 75, 702
AcsI RAATTY 1 cut(s) 775
AcyI GRCGYC 3 cut(s) 9, 168, 180
AgeI ACCGGT 1 cut(s) 734
AgsI TTSAA 1 cut(s) 773
AleI CACNNNNGTG 1 cut(s) 735
AluBI AGCT 5 cut(s) 196, 331, 514, 647, 683
AluI AGCT 5 cut(s) 196, 331, 514, 647, 683
Alw21I GWGCWC 1 cut(s) 445
Alw26I GTCTC 1 cut(s) 539
AoxI GGCC 4 cut(s) 157, 245, 254, 613
ApeKI GCWGC 2 cut(s) 647, 683
ApoI RAATTY 1 cut(s) 775
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AsiGI ACCGGT 1 cut(s) 734
AspLEI GCGC 2 cut(s) 77, 606
AspS9I GGNCC 3 cut(s) 158, 246, 255
AsuC2I CCSGG 2 cut(s) 200, 258
AsuHPI GGTGA 3 cut(s) 97, 615, 752
BanII GRGCYC 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 2 cut(s) 634, 670
BccI CCATC 4 cut(s) 41, 257, 487, 737
BceAI ACGGC 2 cut(s) 167, 249
BcnI CCSGG 2 cut(s) 200, 258
BcoDI GTCTC 1 cut(s) 539
BglI GCCNNNNNGGC 3 cut(s) 165, 253, 612
BisI GCNGC 6 cut(s) 16, 157, 166, 648, 684, 763
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 6 cut(s) 17, 158, 167, 649, 685, 764
Bme1390I CCNGG 2 cut(s) 200, 258
BmgT120I GGNCC 3 cut(s) 158, 246, 255
BmiI GGNNCC 2 cut(s) 97, 160
BmrFI CCNGG 2 cut(s) 200, 258
BpiI GAAGAC 1 cut(s) 545
BplI GAGNNNNNCTC 2 cut(s) 301, 333
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 783
BpuMI CCSGG 2 cut(s) 200, 258
BsaHI GRCGYC 3 cut(s) 9, 168, 180
BsaJI CCNNGG 2 cut(s) 91, 616
BsaWI WCCGGW 1 cut(s) 734
Bse118I RCCGGY 2 cut(s) 243, 734
BseDI CCNNGG 2 cut(s) 91, 616
BseGI GGATG 3 cut(s) 33, 208, 249
BseMII CTCAG 2 cut(s) 458, 489
BseRI GAGGAG 3 cut(s) 59, 469, 644
BseXI GCAGC 2 cut(s) 634, 670
BseYI CCCAGC 1 cut(s) 161
BsgI GTGCAG 3 cut(s) 600, 609, 778
Bsh1236I CGCG 2 cut(s) 75, 702
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 4 cut(s) 159, 247, 256, 615
BshTI ACCGGT 1 cut(s) 734
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 445
BsiSI CCGG 5 cut(s) 199, 244, 253, 258, 735
BslFI GGGAC 1 cut(s) 371
BsmAI GTCTC 1 cut(s) 539
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 4 cut(s) 159, 247, 256, 615
Bsp1286I GDGCHC 2 cut(s) 117, 445
Bsp1720I GCTNAGC 1 cut(s) 510
BspANI GGCC 4 cut(s) 159, 247, 256, 615
BspCNI CTCAG 2 cut(s) 457, 490
BspFNI CGCG 2 cut(s) 75, 702
BspLI GGNNCC 2 cut(s) 97, 160
BsrBI CCGCTC 1 cut(s) 60
BsrFI RCCGGY 2 cut(s) 243, 734
BssAI RCCGGY 2 cut(s) 243, 734
BssECI CCNNGG 2 cut(s) 91, 616
BssNI GRCGYC 3 cut(s) 9, 168, 180
BssT1I CCWWGG 1 cut(s) 616
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 294
BstACI GRCGYC 3 cut(s) 9, 168, 180
BstAPI GCANNNNNTGC 2 cut(s) 587, 792
BstC8I GCNNGC 5 cut(s) 13, 245, 564, 725, 767
BstDEI CTNAG 3 cut(s) 444, 498, 510
BstF5I GGATG 3 cut(s) 33, 208, 249
BstFNI CGCG 2 cut(s) 75, 702
BstHHI GCGC 2 cut(s) 77, 606
BstMAI GTCTC 1 cut(s) 539
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 8 cut(s) 162, 165, 171, 253, 587, 612, 656, 792
BstSCI CCNGG 2 cut(s) 198, 256
BstUI CGCG 2 cut(s) 75, 702
BstV1I GCAGC 2 cut(s) 634, 670
BstV2I GAAGAC 1 cut(s) 545
BsuRI GGCC 4 cut(s) 159, 247, 256, 615
BtsCI GGATG 3 cut(s) 33, 208, 249
Cac8I GCNNGC 5 cut(s) 13, 245, 564, 725, 767
CfoI GCGC 2 cut(s) 77, 606
Cfr10I RCCGGY 2 cut(s) 243, 734
Cfr13I GGNCC 3 cut(s) 158, 246, 255
CseI GACGC 3 cut(s) 17, 157, 188
CspAI ACCGGT 1 cut(s) 734
CviAII CATG 3 cut(s) 397, 410, 751
DdeI CTNAG 3 cut(s) 444, 498, 510
DrdI GACNNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 553
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
EciI GGCGGA 2 cut(s) 16, 88
Eco130I CCWWGG 1 cut(s) 616
Eco24I GRGCYC 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 616
EcoT22I ATGCAT 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 616
FaeI CATG 3 cut(s) 400, 413, 754
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 1 cut(s) 371
FatI CATG 3 cut(s) 396, 409, 750
FauI CCCGC 3 cut(s) 20, 46, 133
FauNDI CATATG 1 cut(s) 688
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 6 cut(s) 16, 157, 166, 648, 684, 763
FokI GGATG 3 cut(s) 20, 215, 236
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 6 cut(s) 16, 157, 166, 648, 684, 763
GlaI GCGC 2 cut(s) 76, 605
GluI GCNGC 6 cut(s) 16, 157, 166, 648, 684, 763
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 4 cut(s) 159, 247, 256, 615
HapII CCGG 5 cut(s) 199, 244, 253, 258, 735
HgaI GACGC 3 cut(s) 17, 157, 188
HhaI GCGC 2 cut(s) 77, 606
Hin1I GRCGYC 3 cut(s) 9, 168, 180
Hin1II CATG 3 cut(s) 400, 413, 754
Hin6I GCGC 2 cut(s) 75, 604
HinP1I GCGC 2 cut(s) 75, 604
HincII GTYRAC 2 cut(s) 208, 268
HindII GTYRAC 2 cut(s) 208, 268
HinfI GANTC 4 cut(s) 83, 370, 470, 554
HpaII CCGG 5 cut(s) 199, 244, 253, 258, 735
HphI GGTGA 3 cut(s) 97, 615, 752
Hpy166II GTNNAC 3 cut(s) 208, 268, 623
Hpy188I TCNGA 5 cut(s) 7, 64, 112, 375, 447
Hpy188III TCNNGA 3 cut(s) 335, 490, 800
Hpy8I GTNNAC 3 cut(s) 208, 268, 623
Hpy99I CGWCG 4 cut(s) 11, 173, 230, 272
HpyAV CCTTC 3 cut(s) 112, 141, 605
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4V TGCA 6 cut(s) 344, 581, 590, 686, 786, 795
HpyF10VI GCNNNNNNNGC 8 cut(s) 162, 165, 171, 253, 587, 612, 656, 792
HpyF3I CTNAG 3 cut(s) 444, 498, 510
Hsp92I GRCGYC 3 cut(s) 9, 168, 180
Hsp92II CATG 3 cut(s) 400, 413, 754
HspAI GCGC 2 cut(s) 75, 604
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
LmnI GCTCC 3 cut(s) 65, 101, 201
Lsp1109I GCAGC 2 cut(s) 634, 670
MaeIII GTNAC 2 cut(s) 229, 691
MbiI CCGCTC 1 cut(s) 60
MboII GAAGA 2 cut(s) 311, 550
MhlI GDGCHC 2 cut(s) 117, 445
MluCI AATT 2 cut(s) 670, 775
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 2 cut(s) 30, 87
Mph1103I ATGCAT 1 cut(s) 346
MroNI GCCGGC 1 cut(s) 243
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 2 cut(s) 324, 735
MspA1I CMGCKG 3 cut(s) 156, 165, 683
MspI CCGG 5 cut(s) 199, 244, 253, 258, 735
MspR9I CCNGG 2 cut(s) 200, 258
MvnI CGCG 2 cut(s) 75, 702
MwoI GCNNNNNNNGC 8 cut(s) 162, 165, 171, 253, 587, 612, 656, 792
NaeI GCCGGC 1 cut(s) 245
NciI CCSGG 2 cut(s) 200, 258
NdeI CATATG 1 cut(s) 688
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 3 cut(s) 400, 413, 754
NlaIV GGNNCC 2 cut(s) 97, 160
NmeAIII GCCGAG 2 cut(s) 72, 632
NmuCI GTSAC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 735
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 245
PfeI GAWTC 3 cut(s) 83, 370, 470
PfoI TCCNGGA 1 cut(s) 198
PinAI ACCGGT 1 cut(s) 734
PkrI GCNGC 6 cut(s) 17, 158, 167, 649, 685, 764
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
PspFI CCCAGC 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 97, 160
PspPI GGNCC 3 cut(s) 158, 246, 255
PvuII CAGCTG 1 cut(s) 683
RseI CAYNNNNRTG 2 cut(s) 324, 735
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 6 cut(s) 16, 157, 166, 648, 684, 763
Sau96I GGNCC 3 cut(s) 158, 246, 255
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 2 cut(s) 200, 258
SduI GDGCHC 2 cut(s) 117, 445
SfiI GGCCNNNNNGGCC 1 cut(s) 253
SgrAI CRCCGGYG 1 cut(s) 734
SmiMI CAYNNNNRTG 2 cut(s) 324, 735
SmlI CTYRAG 1 cut(s) 798
SmoI CTYRAG 1 cut(s) 798
Sse9I AATT 2 cut(s) 670, 775
StyD4I CCNGG 2 cut(s) 198, 256
StyI CCWWGG 1 cut(s) 616
TaaI ACNGT 1 cut(s) 272
TaqI TCGA 4 cut(s) 45, 81, 267, 594
TaqII GACCGA 1 cut(s) 557
TasI AATT 2 cut(s) 670, 775
TauI GCSGC 4 cut(s) 18, 159, 168, 765
TfiI GAWTC 3 cut(s) 83, 370, 470
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 2 cut(s) 647, 683
Tsp45I GTSAC 1 cut(s) 229
XapI RAATTY 1 cut(s) 775
XmiI GTMKAC 1 cut(s) 267
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.