RchiOBHm_Chr4g0431471

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001125
Physical Location & Seq
Reverse (-)
55695227 .. 55698963
3737 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40012

Sequence Viewer

Length: 1533 bp
ATGATCAAGCAGATACTTGGTAGGCTTCCAAGGAAGCCCTCGAAGTCAGCTGACAATCGTGAATTAGGGGGTGCATCTGCATCTTCTTTAAATGCTTCAATCAGTTCAAGAAAGTGTGACTTAGCAAGTGATGGGCCAATAAACGCAAACAGCATACCTGTTTTGGACCTCGATGGTGCTTCGCGTTTTGCAAATCGTGGTGGAAGCAAGCTTCCCCCAGTTGTAAATTCAAAGCTGAATGGTAGTTCTGTGTCTGCTTCTTATGAAGCCTTGCCGGCATTTAGGGATGTGCCTGGCTCTGAGAAGCAGATCTTGTTTATCAAGAAGCTGAATTTGTGTTGCACTGTGTTTGACTTCACTGACCCAGCAAAGCATCTGAAGGAAAAAGACATCAAACGGCAGACATTGCTTGAGCTGGTGGATCATGTTAGTTCTGCCAGTGGAAAATTTAATGAAACTATCATTCAAGAGGCTATAAAGATGGTGAGTGTGAATCTGTTCAGGTCACTCTCTCCACAACCCCGTGAGAACAAGGTTTTGGAATCTTTTGATATGGAGGATGATGAGCCTTTGATGGACCCTGCATGGCCACACTTACAAATTGTGTATGAATTTTTTCTCAGGTTTGTTGCATCTCCTGAGACAGATGCGAAGTTGGCTAAGAGGTACATTGATCAGTCGTTTATTCTCAAGTTGTTGGATCTCTTTGATTCTGAAGATCCTAGGGAGAGAGAGTATCTAAAAACAATTCTGCATCGAATCTATGGGAAGTTTATGGTGCATCGCCCATTCATAAGGAAAGGAATAAACAACATCTTCTTCCGTTTCATTTTTGAAACAGAAAAGCATAATGGGATTGCTGAGATGCTAGAGGTTTTGGGCAGCATAATCAATGGATTTGCTCTTCCACTTAAAGAAGAGCACAAACTCTTTCTTATTCGGGCTCTGATCCCCCTTCACAAACCTAAATGCTTATCAATGTTCCATCAGCAGTTGTCATACTGCATTACTCAGTTCGTAGAGAAAGATTGCAAACTTACAGATACTGTTATAAGAGGCTTGTTAAAGTATTGGCCGATCACTAGTAGTTCAAAGGAAGTCATGTTCTTAAGTGAGTTGGAGGAAATTTTAGAAGCTACCCAGCCTGCAGAATTTCAAAGATGTATGGTACCCCTCTTCCGCCAAATAGCCCGTTGCTTGAGCAGTTCACACTTTCAGGTGGCAGAGAGGGCCTTGTTTTTGTGGAACAATGAACATATTGAGAACTTAATCAAACAAAACCGTAGAGTCATACTGCCCATTATCTTTCCTGCTTTGGAGAAGAATGGCCGCAACCACTGGAACCAAGCTGTACACAGTTTGTCGCTGAACATCCGCAAAATCTTCTCTGATGCTGATCCTGAGCTATTTGAGGAGTGTTCGCTCAAATTTCAGGAAGATGAAGCAAAACTGGAGGAGATTAAAACAAAACAAGAAACCACCTGGAAGCGCTTAGAAGAGATTGCTGCTTCCAAAGCTGTCAGTGGGAAGTAG

Protein Analysis

510

Amino Acids

58.34

Weight (kDa)

8.24

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 91 - 502 8.4e-190 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1052
Acc65I GGTACC 1 cut(s) 1168
AccB1I GGYRCC 1 cut(s) 1168
AccII CGCG 1 cut(s) 184
AciI CCGC 3 cut(s) 1180, 1330, 1375
AclWI GGATC 5 cut(s) 429, 708, 713, 943, 1391
AcoI YGGCCR 3 cut(s) 587, 1073, 1327
AcsI RAATTY 7 cut(s) 226, 331, 446, 611, 1125, 1151, 1427
AcuI CTGAAG 2 cut(s) 398, 735
AfaI GTAC 3 cut(s) 668, 1170, 1353
AfeI AGCGCT 1 cut(s) 1490
AflII CTTAAG 1 cut(s) 1108
AgsI TTSAA 7 cut(s) 99, 108, 231, 467, 836, 1092, 1157
AhlI ACTAGT 1 cut(s) 1082
AjnI CCWGG 2 cut(s) 292, 1481
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
AloI GAACNNNNNNTCC 2 cut(s) 1088, 1120
AluBI AGCT 9 cut(s) 50, 211, 235, 328, 415, 1136, 1349, 1405, 1517
AluI AGCT 9 cut(s) 50, 211, 235, 328, 415, 1136, 1349, 1405, 1517
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 1 cut(s) 635
AlwI GGATC 5 cut(s) 429, 708, 713, 943, 1391
AlwNI CAGNNNCTG 1 cut(s) 1046
Aor51HI AGCGCT 1 cut(s) 1490
AoxI GGCC 5 cut(s) 134, 587, 1073, 1230, 1327
ApeKI GCWGC 2 cut(s) 882, 1505
ApoI RAATTY 7 cut(s) 226, 331, 446, 611, 1125, 1151, 1427
Asp700I GAANNNNTTC 2 cut(s) 497, 615
Asp718I GGTACC 1 cut(s) 1168
AspA2I CCTAGG 1 cut(s) 722
AspLEI GCGC 1 cut(s) 1491
AspS9I GGNCC 4 cut(s) 134, 166, 577, 1230
AsuHPI GGTGA 1 cut(s) 496
AvaII GGWCC 2 cut(s) 166, 577
AvrII CCTAGG 1 cut(s) 722
BalI TGGCCA 1 cut(s) 589
BanI GGYRCC 1 cut(s) 1168
BanII GRGCYC 1 cut(s) 946
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 2 cut(s) 894, 1492
BccI CCATC 5 cut(s) 125, 167, 475, 568, 993
BceAI ACGGC 1 cut(s) 413
BciT130I CCWGG 2 cut(s) 294, 1483
BclI TGATCA 2 cut(s) 3, 673
BcoDI GTCTC 1 cut(s) 635
BcuI ACTAGT 1 cut(s) 1082
BfaI CTAG 3 cut(s) 723, 869, 1083
BfmI CTRYAG 1 cut(s) 1146
BfoI RGCGCY 1 cut(s) 1492
BfrI CTTAAG 1 cut(s) 1108
BglI GCCNNNNNGGC 1 cut(s) 275
BglII AGATCT 1 cut(s) 309
BisI GCNGC 3 cut(s) 883, 1330, 1506
BlnI CCTAGG 1 cut(s) 722
BlsI GCNGC 3 cut(s) 884, 1331, 1507
Bme1390I CCNGG 2 cut(s) 294, 1483
Bme18I GGWCC 2 cut(s) 166, 577
BmgT120I GGNCC 4 cut(s) 134, 166, 577, 1230
BmiI GGNNCC 3 cut(s) 579, 1170, 1343
BmrFI CCNGG 2 cut(s) 294, 1483
BmrI ACTGGG 1 cut(s) 212
BmsI GCATC 9 cut(s) 83, 89, 382, 637, 641, 763, 790, 855, 1381
BmuI ACTGGG 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 1472
Bpu10I CCTNAGC 1 cut(s) 1401
BpuEI CTTGAG 3 cut(s) 431, 674, 1219
BsaBI GATNNNNATC 1 cut(s) 1395
BsaJI CCNNGG 2 cut(s) 29, 722
BsaXI ACNNNNNCTCC 2 cut(s) 719, 749
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 4 cut(s) 218, 438, 1343, 1455
Bse3DI GCAATG 1 cut(s) 404
Bse8I GATNNNNATC 1 cut(s) 1395
BseBI CCWGG 2 cut(s) 294, 1483
BseDI CCNNGG 2 cut(s) 29, 722
BseGI GGATG 3 cut(s) 292, 565, 1371
BseJI GATNNNNATC 1 cut(s) 1395
BseMI GCAATG 1 cut(s) 404
BseMII CTCAG 6 cut(s) 291, 630, 634, 852, 1025, 1392
BseNI ACTGG 4 cut(s) 218, 438, 1343, 1455
BseRI GAGGAG 2 cut(s) 1427, 1469
BseXI GCAGC 2 cut(s) 894, 1492
BseYI CCCAGC 2 cut(s) 364, 1140
Bsh1236I CGCG 1 cut(s) 184
BshFI GGCC 5 cut(s) 136, 589, 1075, 1232, 1329
BshNI GGYRCC 1 cut(s) 1168
BsiHKAI GWGCWC 1 cut(s) 924
BsiSI CCGG 1 cut(s) 275
BsmAI GTCTC 1 cut(s) 635
BsnI GGCC 5 cut(s) 136, 589, 1075, 1232, 1329
Bsp1286I GDGCHC 2 cut(s) 924, 946
Bsp1407I TGTACA 1 cut(s) 1351
Bsp143I GATC 9 cut(s) 3, 309, 421, 673, 700, 718, 948, 1077, 1396
BspACI CCGC 3 cut(s) 1180, 1330, 1375
BspANI GGCC 5 cut(s) 136, 589, 1075, 1232, 1329
BspCNI CTCAG 6 cut(s) 292, 631, 633, 853, 1024, 1393
BspFNI CGCG 1 cut(s) 184
BspLI GGNNCC 3 cut(s) 579, 1170, 1343
BspMAI CTGCAG 1 cut(s) 1150
BspPI GGATC 5 cut(s) 429, 708, 713, 943, 1391
BspQI GCTCTTC 2 cut(s) 909, 912
BspT107I GGYRCC 1 cut(s) 1168
BspTI CTTAAG 1 cut(s) 1108
BsrDI GCAATG 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 1351
BsrI ACTGG 4 cut(s) 218, 438, 1343, 1455
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 2 cut(s) 29, 722
BssMI GATC 9 cut(s) 3, 309, 421, 673, 700, 718, 948, 1077, 1396
BssT1I CCWWGG 2 cut(s) 29, 722
Bst2UI CCWGG 2 cut(s) 294, 1483
Bst4CI ACNGT 4 cut(s) 346, 1048, 1283, 1358
Bst6I CTCTTC 4 cut(s) 909, 912, 1181, 1491
BstAFI CTTAAG 1 cut(s) 1108
BstAPI GCANNNNNTGC 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 1351
BstC8I GCNNGC 3 cut(s) 209, 276, 1146
BstDEI CTNAG 9 cut(s) 121, 300, 620, 639, 660, 861, 1011, 1401, 1492
BstF5I GGATG 3 cut(s) 292, 565, 1371
BstFNI CGCG 1 cut(s) 184
BstH2I RGCGCY 1 cut(s) 1492
BstHHI GCGC 1 cut(s) 1491
BstKTI GATC 9 cut(s) 6, 312, 424, 676, 703, 721, 951, 1080, 1399
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 9 cut(s) 3, 309, 421, 673, 700, 718, 948, 1077, 1396
BstMWI GCNNNNNNNGC 5 cut(s) 275, 406, 656, 1229, 1514
BstNI CCWGG 2 cut(s) 294, 1483
BstSCI CCNGG 2 cut(s) 292, 1481
BstSFI CTRYAG 1 cut(s) 1146
BstUI CGCG 1 cut(s) 184
BstV1I GCAGC 2 cut(s) 894, 1492
BstX2I RGATCY 3 cut(s) 309, 700, 718
BstYI RGATCY 3 cut(s) 309, 700, 718
BsuRI GGCC 5 cut(s) 136, 589, 1075, 1232, 1329
BtgZI GCGATG 1 cut(s) 767
BtsCI GGATG 3 cut(s) 292, 565, 1371
BtsIMutI CAGTG 5 cut(s) 342, 357, 445, 1336, 1528
Cac8I GCNNGC 3 cut(s) 209, 276, 1146
CaiI CAGNNNCTG 1 cut(s) 1046
CfoI GCGC 1 cut(s) 1491
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 4 cut(s) 134, 166, 577, 1230
Csp6I GTAC 3 cut(s) 667, 1169, 1352
CviAII CATG 3 cut(s) 425, 585, 1102
CviQI GTAC 3 cut(s) 667, 1169, 1352
DdeI CTNAG 9 cut(s) 121, 300, 620, 639, 660, 861, 1011, 1401, 1492
DpnI GATC 9 cut(s) 5, 311, 423, 675, 702, 720, 950, 1079, 1398
DpnII GATC 9 cut(s) 3, 309, 421, 673, 700, 718, 948, 1077, 1396
DraI TTTAAA 1 cut(s) 90
EaeI YGGCCR 3 cut(s) 587, 1073, 1327
Eam1104I CTCTTC 4 cut(s) 909, 912, 1181, 1491
EarI CTCTTC 4 cut(s) 909, 912, 1181, 1491
EciI GGCGGA 1 cut(s) 1169
Eco130I CCWWGG 2 cut(s) 29, 722
Eco24I GRGCYC 1 cut(s) 946
Eco47I GGWCC 2 cut(s) 166, 577
Eco47III AGCGCT 1 cut(s) 1490
Eco57I CTGAAG 2 cut(s) 398, 735
EcoO109I RGGNCCY 1 cut(s) 1230
EcoRII CCWGG 2 cut(s) 292, 1481
EcoT14I CCWWGG 2 cut(s) 29, 722
EcoT38I GRGCYC 1 cut(s) 946
ErhI CCWWGG 2 cut(s) 29, 722
FaeI CATG 3 cut(s) 428, 588, 1105
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 296, 328
FatI CATG 3 cut(s) 424, 584, 1101
FbaI TGATCA 2 cut(s) 3, 673
Fnu4HI GCNGC 3 cut(s) 883, 1330, 1506
FokI GGATG 3 cut(s) 299, 572, 1358
FriOI GRGCYC 1 cut(s) 946
Fsp4HI GCNGC 3 cut(s) 883, 1330, 1506
FspBI CTAG 3 cut(s) 723, 869, 1083
GlaI GCGC 1 cut(s) 1490
GluI GCNGC 3 cut(s) 883, 1330, 1506
GsaI CCCAGC 2 cut(s) 368, 1144
GsuI CTGGAG 1 cut(s) 1472
HaeII RGCGCY 1 cut(s) 1492
HaeIII GGCC 5 cut(s) 136, 589, 1075, 1232, 1329
HapII CCGG 1 cut(s) 275
HhaI GCGC 1 cut(s) 1491
Hin1II CATG 3 cut(s) 428, 588, 1105
Hin6I GCGC 1 cut(s) 1489
HinP1I GCGC 1 cut(s) 1489
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 5 cut(s) 493, 542, 710, 759, 1287
HpaII CCGG 1 cut(s) 275
HphI GGTGA 1 cut(s) 496
Hpy166II GTNNAC 2 cut(s) 1208, 1354
Hpy188I TCNGA 5 cut(s) 301, 378, 715, 948, 1390
Hpy188III TCNNGA 7 cut(s) 59, 108, 322, 467, 638, 1400, 1433
Hpy8I GTNNAC 2 cut(s) 1208, 1354
HpyAV CCTTC 2 cut(s) 373, 965
HpyCH4III ACNGT 4 cut(s) 346, 1048, 1283, 1358
HpyF10VI GCNNNNNNNGC 5 cut(s) 275, 406, 656, 1229, 1514
HpyF3I CTNAG 9 cut(s) 121, 300, 620, 639, 660, 861, 1011, 1401, 1492
Hsp92II CATG 3 cut(s) 428, 588, 1105
HspAI GCGC 1 cut(s) 1489
KpnI GGTACC 1 cut(s) 1172
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Ksp22I TGATCA 2 cut(s) 3, 673
Kzo9I GATC 9 cut(s) 3, 309, 421, 673, 700, 718, 948, 1077, 1396
LguI GCTCTTC 2 cut(s) 909, 912
Lsp1109I GCAGC 2 cut(s) 894, 1492
LweI GCATC 9 cut(s) 83, 89, 382, 637, 641, 763, 790, 855, 1381
MaeI CTAG 3 cut(s) 723, 869, 1083
MaeIII GTNAC 2 cut(s) 116, 504
MalI GATC 9 cut(s) 5, 311, 423, 675, 702, 720, 950, 1079, 1398
MboI GATC 9 cut(s) 3, 309, 421, 673, 700, 718, 948, 1077, 1396
MflI RGATCY 3 cut(s) 309, 700, 718
MhlI GDGCHC 2 cut(s) 924, 946
MlsI TGGCCA 1 cut(s) 589
MluNI TGGCCA 1 cut(s) 589
MlyI GAGTC 1 cut(s) 1296
MmeI TCCRAC 2 cut(s) 678, 1098
Mox20I TGGCCA 1 cut(s) 589
MroNI GCCGGC 1 cut(s) 274
MroXI GAANNNNTTC 2 cut(s) 497, 615
MscI TGGCCA 1 cut(s) 589
MseI TTAA 7 cut(s) 89, 450, 912, 1064, 1109, 1268, 1461
Msp20I TGGCCA 1 cut(s) 589
MspA1I CMGCKG 1 cut(s) 50
MspCI CTTAAG 1 cut(s) 1108
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 294, 1483
MvaI CCWGG 2 cut(s) 294, 1483
MvnI CGCG 1 cut(s) 184
MwoI GCNNNNNNNGC 5 cut(s) 275, 406, 656, 1229, 1514
NaeI GCCGGC 1 cut(s) 276
NdeII GATC 9 cut(s) 3, 309, 421, 673, 700, 718, 948, 1077, 1396
NgoMIV GCCGGC 1 cut(s) 274
NlaIII CATG 3 cut(s) 428, 588, 1105
NlaIV GGNNCC 3 cut(s) 579, 1170, 1343
NmuCI GTSAC 2 cut(s) 116, 504
PciSI GCTCTTC 2 cut(s) 909, 912
PdiI GCCGGC 1 cut(s) 276
PdmI GAANNNNTTC 2 cut(s) 497, 615
PfeI GAWTC 4 cut(s) 493, 542, 710, 759
PkrI GCNGC 3 cut(s) 884, 1331, 1507
PleI GAGTC 1 cut(s) 1295
PpsI GAGTC 1 cut(s) 1295
PsiI TTATAA 1 cut(s) 1052
Psp6I CCWGG 2 cut(s) 292, 1481
PspFI CCCAGC 2 cut(s) 364, 1140
PspGI CCWGG 2 cut(s) 292, 1481
PspN4I GGNNCC 3 cut(s) 579, 1170, 1343
PspPI GGNCC 4 cut(s) 134, 166, 577, 1230
PstI CTGCAG 1 cut(s) 1150
PstNI CAGNNNCTG 1 cut(s) 1046
PsuI RGATCY 3 cut(s) 309, 700, 718
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 3 cut(s) 668, 1170, 1353
RsaNI GTAC 3 cut(s) 667, 1169, 1352
SapI GCTCTTC 2 cut(s) 909, 912
SaqAI TTAA 7 cut(s) 89, 450, 912, 1064, 1109, 1268, 1461
SatI GCNGC 3 cut(s) 883, 1330, 1506
Sau3AI GATC 9 cut(s) 3, 309, 421, 673, 700, 718, 948, 1077, 1396
Sau96I GGNCC 4 cut(s) 134, 166, 577, 1230
SchI GAGTC 1 cut(s) 1296
ScrFI CCNGG 2 cut(s) 294, 1483
SduI GDGCHC 2 cut(s) 924, 946
SfaNI GCATC 9 cut(s) 83, 89, 382, 637, 641, 763, 790, 855, 1381
SfcI CTRYAG 1 cut(s) 1146
SinI GGWCC 2 cut(s) 166, 577
SmlI CTYRAG 4 cut(s) 410, 689, 1108, 1198
SmoI CTYRAG 4 cut(s) 410, 689, 1108, 1198
SpeI ACTAGT 1 cut(s) 1082
SsiI CCGC 3 cut(s) 1180, 1330, 1375
SspMI CTAG 3 cut(s) 723, 869, 1083
StyD4I CCNGG 2 cut(s) 292, 1481
StyI CCWWGG 2 cut(s) 29, 722
TaaI ACNGT 4 cut(s) 346, 1048, 1283, 1358
TaqI TCGA 3 cut(s) 41, 171, 757
TatI WGTACW 1 cut(s) 1351
TauI GCSGC 1 cut(s) 1332
TfiI GAWTC 4 cut(s) 493, 542, 710, 759
Tru1I TTAA 7 cut(s) 89, 450, 912, 1064, 1109, 1268, 1461
Tru9I TTAA 7 cut(s) 89, 450, 912, 1064, 1109, 1268, 1461
TscAI CASTG 5 cut(s) 349, 364, 445, 1343, 1528
TseFI GTSAC 2 cut(s) 116, 504
TseI GCWGC 2 cut(s) 882, 1505
Tsp45I GTSAC 2 cut(s) 116, 504
TspDTI ATGAA 7 cut(s) 279, 468, 624, 781, 817, 1266, 1455
TspGWI ACGGA 1 cut(s) 812
TspRI CASTG 5 cut(s) 349, 364, 445, 1343, 1528
Vha464I CTTAAG 1 cut(s) 1108
VpaK11BI GGWCC 2 cut(s) 166, 577
XapI RAATTY 7 cut(s) 226, 331, 446, 611, 1125, 1151, 1427
XmaJI CCTAGG 1 cut(s) 722
XmnI GAANNNNTTC 2 cut(s) 497, 615
XspI CTAG 3 cut(s) 723, 869, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.