Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005841

Physical Location & Seq

Genomic Coordinates Reverse (-)

8793522 .. 8802382

Gene Structure 8861 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29351

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3636 bp

ATGAGTTTGAGCTGCAATTCTGTACCAGAGGCTGAGCCCATGGAACCAGAGACTCCAGATGAAATTCGCCGAGCTTCGCTGGACTTCGATCACTGGACCTCACTCATTTCGCAGATAGAGAATTCCCATCCTGATGATGTAGAGAAAATATGTTCAGTGTATGATTCTTTCTTGGCCGAGTTTCCGTTGTGCCATGGATATTGGAGGAGGTATGCTGAGCATAAGATGCGTTTGTGCTCCATTGACAAGGTTGTTGAGGTCTTTGAACGAGCTCTGCAAGCTGCAACCTACTGTGTTCCGCTGTGGGTGGACTACTGTGTTTTCAGTATGTCCTTTTTCGAAGATCCTTCCGATATTCGCAGACTATTTAAAAGAGGGATGTCCTTTGTTCGAAAGGACTATGGGTGCTATACTTTGTGGGACAAATACATTGAATTTGAGTACTCTCAGCAGGAATGGAGTTCACTGGCCCTGATTTACATCCAAGCTCTTAGATTCCCTACCAAGAAGTTGCATATTTATTATGCGAGTTTGAAGAAGTTGGCTACCTTGTTTGAAGAGGAGATGAAAAGCCAGAGTAATTCTACTGTGGATTTGCAGTCAGAAGCTTTATTTGATGGTGAAGTACCTAGGTTTTTTGGAGATGATGAAATTTCTCTTGTAGTCAAAGACCTTCTGGATCCAGCAATTGGCTCAGGAAGGTCTAAAGCACTGCAAAGATACATATGTATTGGGAAACAGTTGTATCACGAAGCATGCCAGCTTGATGAAAAGATTGGTACTTTTGAGATTAATATAAGGAGGTCTTACTTCCATGTAAAGGAGCTTGATGCGGGTCAGTTGGAGAATTGGCATCGCTATCTGGACTATGTTGAGATGCAAGGGGATTTTGATTGGGCAGTGAAGCTTTACGAAAGGTGCTTGATTCCCTGTGCTAATTACCCCGAGTTTTGGATGCGTTATGTGGATTTCGTGGAAATCAATGGAGGAAGAGAAATTGCAAACTATTCTCTTGACCGAGCAACCCAAATATTTGTGAAGAGAGTGTCGGTGATTCATCTTTTGAATGCCAAGTTTAAGGAGCAAATAGGAGATGTACTTGGTGCCCGTGCGGCTCTTCTTCAATTTAATGCAGAGTCAGATACACACTTCGTTAAGAATGTTATCTTAAAGGCTAATATGGAAAAACGTTTGGGAAATTTCACAGCAGCTTCCACTATATTCAGAGAAGCTCTAGCAATGGCTGCAGAGAAGAAACAGTCGCATACTCTTCCTATTCTATATGTTCATTTCTCTCGCCTTACATTCATGATGACTGATAGTGCAAATGCTGCACGAGATGTCTTGATAGATGGCATCAAACAATTGCCTCACTGCAAAGTACTTCTAGAGGAGTTGATAAAGTTTGCAATGATGAATGGAGGGAAGCAGCACATAAATGTGATAGACTCTGTTGTAACCCGTGCTATATCTCAGGAATCAGGAGTATGCAATGGTCTGAATGCAAAAGATGCTGAGGATATTTCAAGCTTATTTTTAGAGTTTGTTGACCTCTGTGGAACCATCCATGAAGTAAGAAAGGTGTGGACTCAGCATGTACGATTGTTCCCTTCTTCCATTAGGCCATCTTCCTTGGGTCAGCAGTCTGCCTTTAATGTTTATGATCAGCAAGCTACATTCACAAAGTCTTTGAAATTGCCTAGAGAAAAAGAGGAAACTTCTGCTGCTATGACTCAGCAGCCATCTAGAGATTGTACTTCTGACTCCATGATTGAGCTCCCGTTGCATGATGAAGAAATGTTATTGCCAGAAAATCATACTAAAGAGTGTGGCCAGGATCCCGCATACCAGCTATCAGACCAGACATTGCCATCAAAACAATTTGAAAATCTACAGGAAAGATTGCAGCTGACATCTCCGGAAGTTTCAAAAGAGCAGTCCCCAGCTAATGCACCAGAAGCAAATATATCCTCCACCGTGGTAGAAGTGAATCTGGTATCTCAGGAAGTATCTGACAAGGCCCCAGAAAATGCCCCTGTAGCAGAAGTGAAACAGGTATCCCGGGAAGTTTCTGAAGAGGCCACAGAAAATACCCATGTAGCAGAAGTGAAAAAGGTATCTTGGGAAGTTTCTGAGGAGCCCACAGAAAATATCCTTGTAGTAGAAGAGAAAAAAGTGTCTCAGGAAGTTTCTGAGGAGCCCAGAGAAAACATTCCTAAACCAAAGATGTCGTCTGTAGAATTAGGATTTCAAGTTGCTGAAGGAAATGATTCTATAGAATCTTCACAAGAAAACACCATGGGAAGCAATGTTGATCGAGAGTGTGACTATAAGAGTGAACAAGACCCAAAGCCACTGTCCTTGGAGAGTCTTTCTTTGAATTCCGAGGAAAATACAAATTTGGATTTAGTATCCTCCACTTCTCCCAAGTCTGAAGCTTCTCCAGAAACCTGTACTTCAAATGGAAAGAAGCTAGAGAGTGATTGCAACTCTGATCAAGGCTCTTATATGCACAGCCCACGAAATGATAGATCGTTAGAATCAGCTGGAAATGAATTAGTCAATGCTATGCCTATCCCGGTGTTTTCTCAGCCACCTGCAAATAGTTATGGAGACTGGAGTCAAATTAATCGTTCTGATAAAGTTGGTAAAGATTCCAAATTGGGCTTTCGGATGCAGTTACAAAAATCACATCCACAGCAGCATGTTCCTCCAAAGAAATACCCGCGAACTGAAGCAGGTCATCCAATGCCTGCAAGTCAAGGTCACCCCTCTATATTAACACCTTCACAAAACCAACAAATTCAGCAAGGCAGCCAGCAGGCCCAGCATCACAATCAGTATCAGGTAGCAAGTGGTCATGCTAATATAGCAGCACCTAATTCCGGGCCTATACCAAATGTACAAGCCGCCAATGATGCCTCTTCATCTCAGTCTCTATTGCCTGTCCAACCTGTTGCTCCACAGATGCCTCAGTCATCAGTATCAGGCAATGGGCAGTATGCGATGCAGAATCCTCAAGCATATAATCAGATGTGGCAATATTATTACTACCAGCAGCAGCAATCCCTTCTGCAGCCACAGCAGCATCATCCCCAGCAGCAGCAGCAGTTGTTATTGCAACAGTATCAGCAGCAGCAGCAACTTCAACAATATTATGTTCAGATGCAACAGCAACAACTTCTTTTCCAACAACAGCAGCAATTTCAACCAACACAGCAACAACTTCCTTTGCAACAACAGCAACAACTTCCTTTGCAACAACAACAGCAATCTCAAGTACTAAAGCAGCTTCCTTTGCAAGAGCAGCAGCAATTTCAGCAACTACAGCAACAACTTCAGCAATTTCAACAACTGCAGCAACAACTTCCTGTGCAACAACAACAACAATTTCCGTCGCAACAACAATACAATCCGCAATTGCAATTACAACAACAGCATCCATTCTATGTGCATAAGCATCAACTGCCACTCCAACAACAATCACATCTACAGGAACAACATCAAATCAATCAGTCATCAATTCAGCAGAACTACTCACACCATCAGGATCAAGCACAAAATTCAGATATTCATGGTGGGACGGTCATATCCTCCTTATCTCCTCACAGCCCAGTAGAACCTCCCCAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000243 GO:0000245 GO:0000375 GO:0000377 GO:0000395 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005685 GO:0006139 GO:0006376 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0022607 GO:0022613 GO:0022618 GO:0030532 GO:0032991 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0065003 GO:0071004 GO:0071010 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0120114 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1211

Amino Acids

138.29

Weight (kDa)

5.29

Isoelectric Point (pI)

68.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Suf	PF05843	20 - 146	4e-10	Suppressor of forked protein (Suf)
HAT_Syf1_CNRKL1_N	PF23233	20 - 161	5.4e-08	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N	PF23240	21 - 179	9.8e-48	PRP39 N-terminal HAT repeat
HAT_PRP39_C	PF23241	258 - 397	1.9e-41	PRP39 C-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005841)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46400 AT5G46400 AT5G46400
fragaria_vesca	FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350
malus_domestica	MD10G1267400.v1.1
prunus_persica	Prupe.4G074100_v2.0.a1 Prupe.4G074100_v2.0.a1 Prupe.4G074100_v2.0.a1
pyrus_communis	pycom10g22340
rosa_chinensis	RchiOBHm_Chr5g0012961
rosa_laevigata	RLG00000031972
rosa_multiflora	Rmu_sc0006885.1_g000007 Rmu_sc0010837.1_g000003
rosa_roxburghii	Rroxscaffold_1G00063630
rosa_rugosa	Rorug05G0005500
rosa_samantha	Rh5AG099900 Rh5BG096600 Rh5CG108800 Rh5DG095300
rosa_wichuraiana	Rw5G008720 Rw5G008730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2605
Acc36I	ACCTGC	2 cut(s)	2605, 2729
AccB1I	GGYRCC	1 cut(s)	1103
AccB7I	CCANNNNNTGG	1 cut(s)	689
AccII	CGCG	1 cut(s)	2728
AccIII	TCCGGA	1 cut(s)	1918
AciI	CCGC	7 cut(s)	299, 833, 1112, 1842, 2726, 2910, 3416
AclI	AACGTT	1 cut(s)	1189
AclWI	GGATC	6 cut(s)	338, 674, 687, 1832, 1845, 3558
AcoI	YGGCCR	2 cut(s)	174, 1831
AcsI	RAATTY	9 cut(s)	63, 121, 434, 651, 1198, 2380, 2398, 2802, 3562
AcuI	CTGAAG	5 cut(s)	2094, 2280, 2454, 2754, 3323
AfiI	CCNNNNNNNGG	4 cut(s)	689, 820, 951, 2885
AjnI	CCWGG	1 cut(s)	1833
Alw21I	GWGCWC	3 cut(s)	239, 274, 1779
Alw26I	GTCTC	4 cut(s)	44, 2184, 2607, 2940
AlwI	GGATC	6 cut(s)	338, 674, 687, 1832, 1845, 3558
AlwNI	CAGNNNCTG	1 cut(s)	32
Ama87I	CYCGRG	2 cut(s)	944, 2061
Aor13HI	TCCGGA	1 cut(s)	1918
AoxI	GGCC	8 cut(s)	174, 468, 1622, 1831, 2019, 2079, 2823, 2888
ApoI	RAATTY	9 cut(s)	63, 121, 434, 651, 1198, 2380, 2398, 2802, 3562
ArsI	GACNNNNNNTTYG	6 cut(s)	1922, 1954, 2216, 2248, 2342, 2374
AseI	ATTAAT	2 cut(s)	792, 2628
Asp700I	GAANNNNTTC	2 cut(s)	2211, 2269
AspA2I	CCTAGG	1 cut(s)	629
AspS9I	GGNCC	5 cut(s)	96, 469, 2020, 2824, 2888
AsuC2I	CCSGG	4 cut(s)	2062, 2063, 2579, 2887
AsuHPI	GGTGA	3 cut(s)	632, 1063, 2759
AsuII	TTCGAA	2 cut(s)	339, 391
AvaI	CYCGRG	2 cut(s)	944, 2061
AvaII	GGWCC	1 cut(s)	96
AvrII	CCTAGG	1 cut(s)	629
BaeGI	GKGCMC	1 cut(s)	1108
BaeI	ACNNNNGTAYC	4 cut(s)	712, 728, 745, 761
BalI	TGGCCA	1 cut(s)	1833
BamHI	GGATCC	2 cut(s)	679, 1837
BanI	GGYRCC	1 cut(s)	1103
BanII	GRGCYC	5 cut(s)	39, 274, 1779, 2142, 2202
BauI	CACGAG	1 cut(s)	1335
Bbv12I	GWGCWC	3 cut(s)	239, 274, 1779
BbvCI	CCTCAGC	1 cut(s)	1515
BccI	CCATC	8 cut(s)	135, 611, 1346, 1571, 1633, 1750, 1879, 3552
BciT130I	CCWGG	1 cut(s)	1835
BciVI	GTATCC	2 cut(s)	2068, 2422
BclI	TGATCA	2 cut(s)	1663, 2494
BcnI	CCSGG	4 cut(s)	2062, 2063, 2579, 2887
BcoDI	GTCTC	4 cut(s)	44, 2184, 2607, 2940
BfaI	CTAG	6 cut(s)	630, 1235, 1388, 1701, 1746, 2474
BfmI	CTRYAG	9 cut(s)	1245, 1892, 2037, 2235, 2274, 3074, 3326, 3356, 3491
BfuAI	ACCTGC	2 cut(s)	2605, 2729
BfuI	GTATCC	2 cut(s)	2068, 2422
BglI	GCCNNNNNGGC	1 cut(s)	1112
BlnI	CCTAGG	1 cut(s)	629
BlpI	GCTNAGC	2 cut(s)	33, 216
BmcAI	AGTACT	3 cut(s)	443, 1383, 3282
Bme1390I	CCNGG	5 cut(s)	1835, 2062, 2063, 2579, 2887
Bme18I	GGWCC	1 cut(s)	96
BmeT110I	CYCGRG	2 cut(s)	944, 2061
BmgT120I	GGNCC	5 cut(s)	96, 469, 2020, 2824, 2888
BmiI	GGNNCC	8 cut(s)	45, 681, 1105, 1561, 1839, 2022, 2139, 2199
BmrFI	CCNGG	5 cut(s)	1835, 2062, 2063, 2579, 2887
BmrI	ACTGGG	1 cut(s)	3608
BmuI	ACTGGG	1 cut(s)	3608
BoxI	GACNNNNGTC	1 cut(s)	2619
BpmI	CTGGAG	3 cut(s)	39, 2427, 2638
Bpu10I	CCTNAGC	2 cut(s)	694, 1515
Bpu1102I	GCTNAGC	2 cut(s)	33, 216
Bpu14I	TTCGAA	2 cut(s)	339, 391
BpuEI	CTTGAG	2 cut(s)	3003, 3261
BpuMI	CCSGG	4 cut(s)	2062, 2063, 2579, 2887
BsaBI	GATNNNNATC	1 cut(s)	479
BsaJI	CCNNGG	9 cut(s)	39, 193, 629, 1632, 1977, 2061, 2298, 2361, 2385
BsaWI	WCCGGW	1 cut(s)	1918
BsaXI	ACNNNNNCTCC	2 cut(s)	3456, 3486
Bsc4I	CCNNNNNNNGG	4 cut(s)	689, 820, 951, 2885
Bse1I	ACTGG	4 cut(s)	98, 471, 2621, 3614
Bse3DI	GCAATG	6 cut(s)	1245, 1416, 1498, 1865, 2314, 2998
Bse8I	GATNNNNATC	1 cut(s)	479
BseAI	TCCGGA	1 cut(s)	1918
BseBI	CCWGG	1 cut(s)	1835
BseDI	CCNNGG	9 cut(s)	39, 193, 629, 1632, 1977, 2061, 2298, 2361, 2385
BseJI	GATNNNNATC	1 cut(s)	479
BseLI	CCNNNNNNNGG	4 cut(s)	689, 820, 951, 2885
BseMI	GCAATG	6 cut(s)	1245, 1416, 1498, 1865, 2314, 2998
BseNI	ACTGG	4 cut(s)	98, 471, 2621, 3614
BseRI	GAGGAG	6 cut(s)	220, 575, 1406, 2150, 2210, 3594
BseSI	GKGCMC	1 cut(s)	1108
BseYI	CCCAGC	3 cut(s)	1942, 2826, 3096
BsgI	GTGCAG	1 cut(s)	1317
Bsh1236I	CGCG	1 cut(s)	2728
BshFI	GGCC	8 cut(s)	176, 470, 1624, 1833, 2021, 2081, 2825, 2890
BshNI	GGYRCC	1 cut(s)	1103
BsiHKAI	GWGCWC	3 cut(s)	239, 274, 1779
BsiHKCI	CYCGRG	2 cut(s)	944, 2061
BsiSI	CCGG	4 cut(s)	1919, 2062, 2579, 2886
BslFI	GGGAC	3 cut(s)	434, 1924, 3595
BslI	CCNNNNNNNGG	4 cut(s)	689, 820, 951, 2885
BsmAI	GTCTC	4 cut(s)	44, 2184, 2607, 2940
BsmFI	GGGAC	3 cut(s)	434, 1924, 3595
BsmI	GAATGC	2 cut(s)	1072, 1507
BsnI	GGCC	8 cut(s)	176, 470, 1624, 1833, 2021, 2081, 2825, 2890
BsoBI	CYCGRG	2 cut(s)	944, 2061
Bsp119I	TTCGAA	2 cut(s)	339, 391
Bsp1286I	GDGCHC	7 cut(s)	39, 239, 274, 1108, 1779, 2142, 2202
Bsp13I	TCCGGA	1 cut(s)	1918
Bsp1407I	TGTACA	1 cut(s)	2902
Bsp143I	GATC	9 cut(s)	88, 343, 679, 1663, 1837, 2314, 2494, 2531, 3550
Bsp1720I	GCTNAGC	2 cut(s)	33, 216
Bsp19I	CCATGG	3 cut(s)	39, 193, 2298
BspACI	CCGC	7 cut(s)	299, 833, 1112, 1842, 2726, 2910, 3416
BspANI	GGCC	8 cut(s)	176, 470, 1624, 1833, 2021, 2081, 2825, 2890
BspEI	TCCGGA	1 cut(s)	1918
BspFNI	CGCG	1 cut(s)	2728
BspHI	TCATGA	1 cut(s)	1308
BspLI	GGNNCC	8 cut(s)	45, 681, 1105, 1561, 1839, 2022, 2139, 2199
BspMAI	CTGCAG	3 cut(s)	1249, 3078, 3360
BspMI	ACCTGC	2 cut(s)	2605, 2729
BspPI	GGATC	6 cut(s)	338, 674, 687, 1832, 1845, 3558
BspQI	GCTCTTC	1 cut(s)	1122
BspT104I	TTCGAA	2 cut(s)	339, 391
BspT107I	GGYRCC	1 cut(s)	1103
BsrDI	GCAATG	6 cut(s)	1245, 1416, 1498, 1865, 2314, 2998
BsrGI	TGTACA	1 cut(s)	2902
BsrI	ACTGG	4 cut(s)	98, 471, 2621, 3614
BssECI	CCNNGG	9 cut(s)	39, 193, 629, 1632, 1977, 2061, 2298, 2361, 2385
BssMI	GATC	9 cut(s)	88, 343, 679, 1663, 1837, 2314, 2494, 2531, 3550
BssSI	CACGAG	1 cut(s)	1335
BssT1I	CCWWGG	6 cut(s)	39, 193, 629, 1632, 2298, 2361
Bst2BI	CACGAG	1 cut(s)	1335
Bst2UI	CCWGG	1 cut(s)	1835
Bst4CI	ACNGT	9 cut(s)	293, 317, 589, 741, 1260, 1978, 2358, 3126, 3586
Bst6I	CTCTTC	8 cut(s)	552, 985, 1034, 1122, 1275, 2070, 2160, 2929
BstAPI	GCANNNNNTGC	5 cut(s)	226, 1244, 1331, 1511, 3466
BstAUI	TGTACA	1 cut(s)	2902
BstBI	TTCGAA	2 cut(s)	339, 391
BstC8I	GCNNGC	7 cut(s)	279, 757, 761, 1671, 2754, 2819, 2823
BstDSI	CCRYGG	4 cut(s)	39, 193, 1977, 2298
BstEII	GGTNACC	1 cut(s)	2765
BstFNI	CGCG	1 cut(s)	2728
BstKTI	GATC	9 cut(s)	91, 346, 682, 1666, 1840, 2317, 2497, 2534, 3553
BstMAI	GTCTC	4 cut(s)	44, 2184, 2607, 2940
BstMBI	GATC	9 cut(s)	88, 343, 679, 1663, 1837, 2314, 2494, 2531, 3550
BstNI	CCWGG	1 cut(s)	1835
BstNSI	RCATGY	3 cut(s)	759, 1598, 2708
BstPAI	GACNNNNGTC	1 cut(s)	2619
BstPI	GGTNACC	1 cut(s)	2765
BstSCI	CCNGG	5 cut(s)	1833, 2060, 2061, 2577, 2885
BstSFI	CTRYAG	9 cut(s)	1245, 1892, 2037, 2235, 2274, 3074, 3326, 3356, 3491
BstSLI	GKGCMC	1 cut(s)	1108
BstUI	CGCG	1 cut(s)	2728
BstX2I	RGATCY	3 cut(s)	343, 679, 1837
BstYI	RGATCY	3 cut(s)	343, 679, 1837
BsuI	GTATCC	2 cut(s)	2068, 2422
BsuRI	GGCC	8 cut(s)	176, 470, 1624, 1833, 2021, 2081, 2825, 2890
BtgI	CCRYGG	4 cut(s)	39, 193, 1977, 2298
BtgZI	GCGATG	2 cut(s)	839, 3020
BtsI	GCAGTG	3 cut(s)	710, 906, 1372
BtsIMutI	CAGTG	7 cut(s)	91, 162, 464, 710, 906, 1372, 2354
BveI	ACCTGC	2 cut(s)	2605, 2729
Cac8I	GCNNGC	7 cut(s)	279, 757, 761, 1671, 2754, 2819, 2823
CaiI	CAGNNNCTG	1 cut(s)	32
CciI	TCATGA	1 cut(s)	1308
Cfr13I	GGNCC	5 cut(s)	96, 469, 2020, 2824, 2888
Cfr9I	CCCGGG	1 cut(s)	2061
CspCI	CAANNNNNGTGG	2 cut(s)	2343, 2378
DpnI	GATC	9 cut(s)	90, 345, 681, 1665, 1839, 2316, 2496, 2533, 3552
DpnII	GATC	9 cut(s)	88, 343, 679, 1663, 1837, 2314, 2494, 2531, 3550
DraI	TTTAAA	1 cut(s)	370
EaeI	YGGCCR	2 cut(s)	174, 1831
Eam1104I	CTCTTC	8 cut(s)	552, 985, 1034, 1122, 1275, 2070, 2160, 2929
EarI	CTCTTC	8 cut(s)	552, 985, 1034, 1122, 1275, 2070, 2160, 2929
Ecl136II	GAGCTC	2 cut(s)	272, 1777
Eco130I	CCWWGG	6 cut(s)	39, 193, 629, 1632, 2298, 2361
Eco24I	GRGCYC	5 cut(s)	39, 274, 1779, 2142, 2202
Eco47I	GGWCC	1 cut(s)	96
Eco53kI	GAGCTC	2 cut(s)	272, 1777
Eco57I	CTGAAG	5 cut(s)	2094, 2280, 2454, 2754, 3323
Eco88I	CYCGRG	2 cut(s)	944, 2061
Eco91I	GGTNACC	1 cut(s)	2765
EcoICRI	GAGCTC	2 cut(s)	272, 1777
EcoO109I	RGGNCCY	1 cut(s)	2020
EcoO65I	GGTNACC	1 cut(s)	2765
EcoRI	GAATTC	2 cut(s)	121, 2380
EcoRII	CCWGG	1 cut(s)	1833
EcoT14I	CCWWGG	6 cut(s)	39, 193, 629, 1632, 2298, 2361
EcoT38I	GRGCYC	5 cut(s)	39, 274, 1779, 2142, 2202
ErhI	CCWWGG	6 cut(s)	39, 193, 629, 1632, 2298, 2361
FalI	AAGNNNNNCTT	2 cut(s)	790, 822
FaqI	GGGAC	3 cut(s)	434, 1924, 3595
FauI	CCCGC	3 cut(s)	826, 1849, 2733
FauNDI	CATATG	1 cut(s)	725
FbaI	TGATCA	2 cut(s)	1663, 2494
FriOI	GRGCYC	5 cut(s)	39, 274, 1779, 2142, 2202
FspBI	CTAG	6 cut(s)	630, 1235, 1388, 1701, 1746, 2474
GsaI	CCCAGC	3 cut(s)	1946, 2830, 3100
GsuI	CTGGAG	3 cut(s)	39, 2427, 2638
HaeIII	GGCC	8 cut(s)	176, 470, 1624, 1833, 2021, 2081, 2825, 2890
HapII	CCGG	4 cut(s)	1919, 2062, 2579, 2886
HincII	GTYRAC	1 cut(s)	1549
HindII	GTYRAC	1 cut(s)	1549
HindIII	AAGCTT	4 cut(s)	606, 905, 1528, 2436
HpaII	CCGG	4 cut(s)	1919, 2062, 2579, 2886
HphI	GGTGA	3 cut(s)	632, 1063, 2759
Hpy166II	GTNNAC	5 cut(s)	310, 464, 1549, 1587, 2339
Hpy8I	GTNNAC	5 cut(s)	310, 464, 1549, 1587, 2339
Hpy99I	CGWCG	1 cut(s)	3400
HpyAV	CCTTC	7 cut(s)	357, 683, 693, 1620, 2255, 2796, 3080
HpyCH4III	ACNGT	9 cut(s)	293, 317, 589, 741, 1260, 1978, 2358, 3126, 3586
HpyCH4IV	ACGT	1 cut(s)	1189
HpySE526I	ACGT	1 cut(s)	1189
Kpn2I	TCCGGA	1 cut(s)	1918
Ksp22I	TGATCA	2 cut(s)	1663, 2494
Kzo9I	GATC	9 cut(s)	88, 343, 679, 1663, 1837, 2314, 2494, 2531, 3550
LguI	GCTCTTC	1 cut(s)	1122
LmnI	GCTCC	7 cut(s)	242, 823, 1081, 1782, 2137, 2197, 2965
MaeI	CTAG	6 cut(s)	630, 1235, 1388, 1701, 1746, 2474
MaeII	ACGT	1 cut(s)	1189
MaeIII	GTNAC	4 cut(s)	1456, 2324, 2679, 2765
MalI	GATC	9 cut(s)	90, 345, 681, 1665, 1839, 2316, 2496, 2533, 3552
MboI	GATC	9 cut(s)	88, 343, 679, 1663, 1837, 2314, 2494, 2531, 3550
MfeI	CAATTG	3 cut(s)	687, 1364, 3419
MflI	RGATCY	3 cut(s)	343, 679, 1837
MhlI	GDGCHC	7 cut(s)	39, 239, 274, 1108, 1779, 2142, 2202
MlsI	TGGCCA	1 cut(s)	1833
MluNI	TGGCCA	1 cut(s)	1833
MlyI	GAGTC	8 cut(s)	46, 1145, 1442, 1582, 1726, 1757, 2377, 2629
MmeI	TCCRAC	4 cut(s)	822, 2974, 3214, 3499
Mox20I	TGGCCA	1 cut(s)	1833
MroI	TCCGGA	1 cut(s)	1918
MroXI	GAANNNNTTC	2 cut(s)	2211, 2269
MscI	TGGCCA	1 cut(s)	1833
MseI	TTAA	9 cut(s)	369, 792, 1077, 1128, 1155, 1169, 1653, 2628, 2780
MslI	CAYNNNNRTG	3 cut(s)	132, 1437, 1439
Msp20I	TGGCCA	1 cut(s)	1833
MspA1I	CMGCKG	3 cut(s)	301, 1909, 2546
MspI	CCGG	4 cut(s)	1919, 2062, 2579, 2886
MspR9I	CCNGG	5 cut(s)	1835, 2062, 2063, 2579, 2887
MunI	CAATTG	3 cut(s)	687, 1364, 3419
Mva1269I	GAATGC	2 cut(s)	1072, 1507
MvaI	CCWGG	1 cut(s)	1835
MvnI	CGCG	1 cut(s)	2728
NciI	CCSGG	4 cut(s)	2062, 2063, 2579, 2887
NcoI	CCATGG	3 cut(s)	39, 193, 2298
NdeI	CATATG	1 cut(s)	725
NdeII	GATC	9 cut(s)	88, 343, 679, 1663, 1837, 2314, 2494, 2531, 3550
NlaIV	GGNNCC	8 cut(s)	45, 681, 1105, 1561, 1839, 2022, 2139, 2199
NmeAIII	GCCGAG	2 cut(s)	95, 202
NmuCI	GTSAC	2 cut(s)	2324, 2765
NspI	RCATGY	3 cut(s)	759, 1598, 2708
NspV	TTCGAA	2 cut(s)	339, 391
PaeI	GCATGC	1 cut(s)	759
PagI	TCATGA	1 cut(s)	1308
PaqCI	CACCTGC	1 cut(s)	2605
PciSI	GCTCTTC	1 cut(s)	1122
PctI	GAATGC	2 cut(s)	1072, 1507
PdmI	GAANNNNTTC	2 cut(s)	2211, 2269
PflMI	CCANNNNNTGG	1 cut(s)	689
PleI	GAGTC	8 cut(s)	46, 1144, 1442, 1582, 1726, 1757, 2376, 2628
PpsI	GAGTC	8 cut(s)	46, 1144, 1442, 1582, 1726, 1757, 2376, 2628
PshAI	GACNNNNGTC	1 cut(s)	2619
PshBI	ATTAAT	2 cut(s)	792, 2628
Psp124BI	GAGCTC	2 cut(s)	274, 1779
Psp1406I	AACGTT	1 cut(s)	1189
Psp6I	CCWGG	1 cut(s)	1833
PspEI	GGTNACC	1 cut(s)	2765
PspFI	CCCAGC	3 cut(s)	1942, 2826, 3096
PspGI	CCWGG	1 cut(s)	1833
PspN4I	GGNNCC	8 cut(s)	45, 681, 1105, 1561, 1839, 2022, 2139, 2199
PspPI	GGNCC	5 cut(s)	96, 469, 2020, 2824, 2888
PstI	CTGCAG	3 cut(s)	1249, 3078, 3360
PstNI	CAGNNNCTG	1 cut(s)	32
PsuI	RGATCY	3 cut(s)	343, 679, 1837
PvuII	CAGCTG	2 cut(s)	1909, 2546
RseI	CAYNNNNRTG	3 cut(s)	132, 1437, 1439
SacI	GAGCTC	2 cut(s)	274, 1779
SapI	GCTCTTC	1 cut(s)	1122
SaqAI	TTAA	9 cut(s)	369, 792, 1077, 1128, 1155, 1169, 1653, 2628, 2780
Sau3AI	GATC	9 cut(s)	88, 343, 679, 1663, 1837, 2314, 2494, 2531, 3550
Sau96I	GGNCC	5 cut(s)	96, 469, 2020, 2824, 2888
ScaI	AGTACT	3 cut(s)	443, 1383, 3282
SchI	GAGTC	8 cut(s)	46, 1145, 1442, 1582, 1726, 1757, 2377, 2629
ScrFI	CCNGG	5 cut(s)	1835, 2062, 2063, 2579, 2887
SduI	GDGCHC	7 cut(s)	39, 239, 274, 1108, 1779, 2142, 2202
SfcI	CTRYAG	9 cut(s)	1245, 1892, 2037, 2235, 2274, 3074, 3326, 3356, 3491
SfuI	TTCGAA	2 cut(s)	339, 391
SinI	GGWCC	1 cut(s)	96
SmaI	CCCGGG	1 cut(s)	2063
SmiMI	CAYNNNNRTG	3 cut(s)	132, 1437, 1439
SmlI	CTYRAG	2 cut(s)	3018, 3276
SmoI	CTYRAG	2 cut(s)	3018, 3276
SphI	GCATGC	1 cut(s)	759
SsiI	CCGC	7 cut(s)	299, 833, 1112, 1842, 2726, 2910, 3416
SspI	AATATT	3 cut(s)	1032, 3044, 3155
SspMI	CTAG	6 cut(s)	630, 1235, 1388, 1701, 1746, 2474
SstI	GAGCTC	2 cut(s)	274, 1779
StyD4I	CCNGG	5 cut(s)	1833, 2060, 2061, 2577, 2885
StyI	CCWWGG	6 cut(s)	39, 193, 629, 1632, 2298, 2361
TaaI	ACNGT	9 cut(s)	293, 317, 589, 741, 1260, 1978, 2358, 3126, 3586
TaiI	ACGT	1 cut(s)	1192
TaqI	TCGA	4 cut(s)	87, 339, 391, 2317
TaqII	GACCGA	1 cut(s)	1032
TatI	WGTACW	7 cut(s)	441, 1096, 1381, 1754, 2453, 2902, 3280
TauI	GCSGC	2 cut(s)	1115, 2912
Tru1I	TTAA	9 cut(s)	369, 792, 1077, 1128, 1155, 1169, 1653, 2628, 2780
Tru9I	TTAA	9 cut(s)	369, 792, 1077, 1128, 1155, 1169, 1653, 2628, 2780
TscAI	CASTG	7 cut(s)	98, 162, 471, 717, 906, 1379, 2361
TseFI	GTSAC	2 cut(s)	2324, 2765
Tsp45I	GTSAC	2 cut(s)	2324, 2765
TspGWI	ACGGA	2 cut(s)	174, 3384
TspMI	CCCGGG	1 cut(s)	2061
TspRI	CASTG	7 cut(s)	98, 162, 471, 717, 906, 1379, 2361
Van91I	CCANNNNNTGG	1 cut(s)	689
VpaK11BI	GGWCC	1 cut(s)	96
VspI	ATTAAT	2 cut(s)	792, 2628
XapI	RAATTY	9 cut(s)	63, 121, 434, 651, 1198, 2380, 2398, 2802, 3562
XbaI	TCTAGA	2 cut(s)	1387, 1745
XceI	RCATGY	3 cut(s)	759, 1598, 2708
XmaI	CCCGGG	1 cut(s)	2061
XmaJI	CCTAGG	1 cut(s)	629
XmnI	GAANNNNTTC	2 cut(s)	2211, 2269
XspI	CTAG	6 cut(s)	630, 1235, 1388, 1701, 1746, 2474
ZrmI	AGTACT	3 cut(s)	443, 1383, 3282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.