Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005841

Physical Location & Seq

Genomic Coordinates Forward (+)

85436995 .. 85444232

Gene Structure 7238 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00063630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3021 bp

ATGAGTTTGAGCTGCAATTCTGTACCAGAGGCTGAGCCCATGGAACCAGAGACTCCAGATGAAATTGGCCGAGCTTCGCTGGACTTCGATCACTGGACCTCACTCATTTCGCAGATAGAGAATTCCTATCCTGATGATATAGAGAAAATTTGCTCAGTGTATGATTCTTTTTTGGCCGAGTTTCCGTTGTGCCATGGATATTGGAGGAGGTATGCTGAGCATAAGATGCGTTTGTGCTCCATTGACAAGGTTGTTGAGGTCTTTGAACGAGCTCTGCAAGCTGCAACCTACTGTGTTCCGCTGTGGGTGGACTACTGTGTTTTCAGTATGTCCTTTTTCGAAGATCCTTCCGATATTCGCAGACTATTTAAAAGAGGGATGCCCTTTGTTCGAAAGGACTATGGGTGCTATACTTTGTGGGACAAATACATTGAATTTGAGTACTCTCAGCAGGAATGGAGTTCACTGGCCCTGATTTACATCCAAGCTCTTAGATTCCCTACCAAGAAGTTGCATATTTATTATGCGAGTTTGAAGAAGTTGGCTACCTTGTTTGAAGAGGAGATGAAAAGCCAGAGTAATTCTACTGTGGATTTGCAGTCAGAAGCTTTATTTGATGGTGAAGTCCCTAGGTTTTTTGGAGATGATGAAATTTCTCTTGTAGTCAAAGACCTTCTGGATCCAGCAATTGGCTCAGGAAGGTCTAAAGCACTGCAAAGATATATATGTATTGGGAAACAGTTGTATCACGAAGCATGCCAGCTTGATGAAAAGATTGGTACTTTTGAGATTAATATAAGGAGGTCTTACTTCCATGTAAAGGAGCTTGATGCGGGTCAGTTGGAGAATTGGCATCGCTATCTGGACTATGTTGAGATGCAAGGGGATTTTGATTGGGCAGTGAAGCTTTACGAGAGGTGCTTGATTCCCTGTGCTAATTACCCCGAGTTTTGGATGCGTTATGTGGATTTCGTGGAAATCAATGGAGGAAGAGAAATTGCAAACTATTCTCTTGACCGAGCAACCCAAATATTTGTGAAGAGAGTGTCGGTGATTCATCTTTTGAATGCCAAGTTTAAGGAGCAAATAGGAGATGTACTTGGTGCCCGTGCGGCTCTTCTTCAATGTAATGCAGAGTCAGATACACACTTCGTTAAGAATGTTATCTTAAAGGCTAATATGGAAAAACGTTTGGGAAATTTCACAGCAGCTTCCACTATATTCAGAGAAGCTCTAGCAATGGCTGCAGAGAAGAAACAGTCGCATACTCTTCCTATTCTATATGTTCATTTCTCTCGCCTTACATTCATGGAGTTGATAAAGTTTGCAATGATGAATGGAGGGAAGCAGCACATAAATGTGATAGACTCTGTTGTAACCCATGCTATATCTCAGGAATCAGGAGTATGCAATGGTCTGAATGCAAAGGATGCTGAGGATATTTCAAGCTTATTTTTAGAGTTTGTTGACCTCTGTGGAGCCATCCATGAAGTAAGAAAGGTGTGGACTCGGCATGTACGATTGTTCCCTTCTTCCATTAGGCCATCTTCCTTGGGTCAGCAGTCTGCCTTTAATGTTTATGATCAGCAAGCTACATTCACAAAGTCATTGAATTTGCCTAGAGAAAAAGATGAAACTTCTGCTGCTATGCCTCAGCAGCCATCTAGAGATTGTACTTCTGACTCCATGATTGAGCTCCCGTTGCATGATGAAGAAATGTTATTGCCAGAAAATCATACTAAAGAGTGTGGCCAGGATCCCGCATACCAGCTATCAGACCAGACATTGCCATCAAAACAATTTGAAAATCTACAGGAAAGATTGCAGCTAACATCTCCGGAAGTTTCAAGAGAGCAGTCCCCAGGTAATGCACCAGAAGCAAATATGTCCTCCACCATGGTAGAAGTGAATCTGGTATCTCAGGAAGTATCTGACAAGGCCCCCGAAAATGCCCCTGTAGCAGAAGTGAAACAGGTATTCTGGGAAGTTTCTGAGGAGCGCACAGAAAATATCCTTGTAGCAGAAGTGAAAAAGGTATCTCGGGAAGTTTCTGAGGAGCGCACAGAAAATATCCTTGTAGTAGAAGAGAAAAAAGTATCTCAGGAAGTTTCTGAGGAGGCCAGAGAAAACATCCCTAAACCAAAGATGTTGTCTGTAGAATTAGGATTTCAAGTTGCTGAAGGAAATGATTCTATAGAATCTTCACAAGAAAACACCATGGGAAGCAATGTTGATCGAGAGTGTGACTGTAAGAGTGAACAAGACCCAAAGCCACTGTCCTTGGAGAGTCTTTCTTTGAATTCCCAGGAAAATACAAATTTGGATTTAGTACCCTCCACTTCTCCCAAGTCTGAAGCTTCTCCAGAAACCTGTACTTCAAATGGAAAGAAGCTAGAGAGTGATCAAGACTCTTATATGCACAGCCCACGAAATGATAGATCATCAGAATCAGCTGGAAATGAATTAGTCAATGCTATGCCTATCCCGGTGTTTTCTCAGCCACCTGCAAATAGTTATGGAGACTGGAGTCAAATTAATCGTTCTGATAAAGTTGGTAAAGATTCCAAATTGGGCTTTCGGATGCAGTTACAAAGAAAATCACATCCACAGCAGCATGTTCCTCCACAGAAATACCCGCGAACTGAAGCAGGTCATCCAATGCCTGCAAGTCAAGGTCACCCCTCTATATTAGCACCTTCACAAAACCAACAAATTCAGCAAGGCAGCCAGCAGGCCCAGCATCACAATCAGTATCAGGTAGCAAGTGGTCATGCTAATATAGCAGCACCTAATTCCGGGCCTGTACCAAATGTACAAGCCGCCAATGATGCCTCTTCATCTCAGTCTCTATTGCCTGTCCAACCTGTTGCTCCACAGATGCCTCAGTCATCAGTATTAGGATCAAGCACAAAATTCAGATATTCATGGTGGGACGGTCATATCCTCCTTATCTCCTCACAGCCCAGTAGAACCTCCCCAGAAGTGAAGGCAGATGCCGTTTGCTTTTTATCTACTTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000243 GO:0000245 GO:0000375 GO:0000377 GO:0000395 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005685 GO:0006139 GO:0006376 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0022607 GO:0022613 GO:0022618 GO:0030532 GO:0032991 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0065003 GO:0071004 GO:0071010 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0120114 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1006

Amino Acids

113.6

Weight (kDa)

5.15

Isoelectric Point (pI)

62.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Suf	PF05843	20 - 108	1.3e-09	Suppressor of forked protein (Suf)
HAT_Syf1_CNRKL1_N	PF23233	21 - 127	1e-05	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N	PF23240	24 - 179	2.8e-47	PRP39 N-terminal HAT repeat
HAT_PRP39_C	PF23241	258 - 397	2.5e-41	PRP39 C-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005841)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46400 AT5G46400 AT5G46400
fragaria_vesca	FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350 FvH4_3g06350
malus_domestica	MD10G1267400.v1.1
prunus_persica	Prupe.4G074100_v2.0.a1 Prupe.4G074100_v2.0.a1 Prupe.4G074100_v2.0.a1
pyrus_communis	pycom10g22340
rosa_chinensis	RchiOBHm_Chr5g0012961
rosa_laevigata	RLG00000031972
rosa_multiflora	Rmu_sc0006885.1_g000007 Rmu_sc0010837.1_g000003
rosa_roxburghii	Rroxscaffold_1G00063630
rosa_rugosa	Rorug05G0005500
rosa_samantha	Rh5AG099900 Rh5BG096600 Rh5CG108800 Rh5DG095300
rosa_wichuraiana	Rw5G008720 Rw5G008730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2512
Acc36I	ACCTGC	2 cut(s)	2512, 2639
AccB1I	GGYRCC	1 cut(s)	1103
AccB7I	CCANNNNNTGG	1 cut(s)	689
AccII	CGCG	1 cut(s)	2638
AccIII	TCCGGA	1 cut(s)	1837
AciI	CCGC	6 cut(s)	299, 833, 1112, 1761, 2636, 2820
AclI	AACGTT	1 cut(s)	1189
AclWI	GGATC	6 cut(s)	338, 674, 687, 1751, 1764, 2908
AcoI	YGGCCR	3 cut(s)	67, 174, 1750
AcuI	CTGAAG	3 cut(s)	2199, 2373, 2664
AfiI	CCNNNNNNNGG	4 cut(s)	689, 820, 951, 2795
AjnI	CCWGG	3 cut(s)	1752, 1861, 2304
Alw21I	GWGCWC	3 cut(s)	239, 274, 1698
Alw26I	GTCTC	3 cut(s)	44, 2514, 2850
AlwI	GGATC	6 cut(s)	338, 674, 687, 1751, 1764, 2908
AlwNI	CAGNNNCTG	1 cut(s)	32
Ama87I	CYCGRG	2 cut(s)	944, 2040
Aor13HI	TCCGGA	1 cut(s)	1837
AoxI	GGCC	9 cut(s)	67, 174, 468, 1541, 1750, 1938, 2118, 2733, 2798
ArsI	GACNNNNNNTTYG	4 cut(s)	2135, 2167, 2261, 2293
AseI	ATTAAT	2 cut(s)	792, 2535
Asp700I	GAANNNNTTC	1 cut(s)	2188
AspA2I	CCTAGG	1 cut(s)	629
AspLEI	GCGC	2 cut(s)	2001, 2061
AspS9I	GGNCC	5 cut(s)	96, 469, 1939, 2734, 2798
AsuC2I	CCSGG	2 cut(s)	2486, 2797
AsuHPI	GGTGA	3 cut(s)	632, 1063, 2669
AsuII	TTCGAA	2 cut(s)	339, 391
AvaI	CYCGRG	2 cut(s)	944, 2040
AvaII	GGWCC	1 cut(s)	96
AvrII	CCTAGG	1 cut(s)	629
BaeGI	GKGCMC	1 cut(s)	1108
BaeI	ACNNNNGTAYC	2 cut(s)	728, 761
BalI	TGGCCA	1 cut(s)	1752
BamHI	GGATCC	2 cut(s)	679, 1756
BanI	GGYRCC	1 cut(s)	1103
BanII	GRGCYC	3 cut(s)	39, 274, 1698
Bbv12I	GWGCWC	3 cut(s)	239, 274, 1698
BbvCI	CCTCAGC	2 cut(s)	1434, 1653
BccI	CCATC	5 cut(s)	611, 1490, 1552, 1669, 1798
BceAI	ACGGC	1 cut(s)	2981
BciT130I	CCWGG	3 cut(s)	1754, 1863, 2306
BclI	TGATCA	2 cut(s)	1582, 2401
BcnI	CCSGG	2 cut(s)	2486, 2797
BcoDI	GTCTC	3 cut(s)	44, 2514, 2850
BfaI	CTAG	5 cut(s)	630, 1235, 1620, 1665, 2393
BfmI	CTRYAG	5 cut(s)	1245, 1811, 1956, 2154, 2193
BfuAI	ACCTGC	2 cut(s)	2512, 2639
BglI	GCCNNNNNGGC	1 cut(s)	1112
BlnI	CCTAGG	1 cut(s)	629
BlpI	GCTNAGC	2 cut(s)	33, 216
BmcAI	AGTACT	1 cut(s)	443
Bme1390I	CCNGG	5 cut(s)	1754, 1863, 2306, 2486, 2797
Bme18I	GGWCC	1 cut(s)	96
BmeT110I	CYCGRG	2 cut(s)	944, 2040
BmgT120I	GGNCC	5 cut(s)	96, 469, 1939, 2734, 2798
BmiI	GGNNCC	6 cut(s)	45, 681, 1105, 1480, 1758, 1941
BmrFI	CCNGG	5 cut(s)	1754, 1863, 2306, 2486, 2797
BmrI	ACTGGG	1 cut(s)	2958
BmuI	ACTGGG	1 cut(s)	2958
BoxI	GACNNNNGTC	1 cut(s)	2526
BpmI	CTGGAG	3 cut(s)	39, 2346, 2545
Bpu10I	CCTNAGC	3 cut(s)	694, 1434, 1653
Bpu1102I	GCTNAGC	2 cut(s)	33, 216
Bpu14I	TTCGAA	2 cut(s)	339, 391
BpuMI	CCSGG	2 cut(s)	2486, 2797
BsaBI	GATNNNNATC	1 cut(s)	479
BsaJI	CCNNGG	9 cut(s)	39, 193, 629, 1551, 1861, 1896, 2217, 2280, 2304
BsaWI	WCCGGW	1 cut(s)	1837
Bsc4I	CCNNNNNNNGG	4 cut(s)	689, 820, 951, 2795
Bse1I	ACTGG	4 cut(s)	98, 471, 2528, 2964
Bse3DI	GCAATG	5 cut(s)	1245, 1335, 1417, 1784, 2233
Bse8I	GATNNNNATC	1 cut(s)	479
BseAI	TCCGGA	1 cut(s)	1837
BseBI	CCWGG	3 cut(s)	1754, 1863, 2306
BseDI	CCNNGG	9 cut(s)	39, 193, 629, 1551, 1861, 1896, 2217, 2280, 2304
BseGI	GGATG	9 cut(s)	384, 480, 960, 1435, 1482, 2130, 2586, 2602, 2653
BseJI	GATNNNNATC	1 cut(s)	479
BseLI	CCNNNNNNNGG	4 cut(s)	689, 820, 951, 2795
BseMI	GCAATG	5 cut(s)	1245, 1335, 1417, 1784, 2233
BseNI	ACTGG	4 cut(s)	98, 471, 2528, 2964
BseRI	GAGGAG	6 cut(s)	220, 575, 2009, 2069, 2129, 2944
BseSI	GKGCMC	1 cut(s)	1108
BseYI	CCCAGC	1 cut(s)	2736
Bsh1236I	CGCG	1 cut(s)	2638
BshFI	GGCC	9 cut(s)	69, 176, 470, 1543, 1752, 1940, 2120, 2735, 2800
BshNI	GGYRCC	1 cut(s)	1103
BsiHKAI	GWGCWC	3 cut(s)	239, 274, 1698
BsiHKCI	CYCGRG	2 cut(s)	944, 2040
BsiSI	CCGG	3 cut(s)	1838, 2486, 2796
BslFI	GGGAC	4 cut(s)	434, 611, 1843, 2945
BslI	CCNNNNNNNGG	4 cut(s)	689, 820, 951, 2795
BsmAI	GTCTC	3 cut(s)	44, 2514, 2850
BsmFI	GGGAC	4 cut(s)	434, 611, 1843, 2945
BsmI	GAATGC	2 cut(s)	1072, 1426
BsnI	GGCC	9 cut(s)	69, 176, 470, 1543, 1752, 1940, 2120, 2735, 2800
BsoBI	CYCGRG	2 cut(s)	944, 2040
Bsp119I	TTCGAA	2 cut(s)	339, 391
Bsp1286I	GDGCHC	5 cut(s)	39, 239, 274, 1108, 1698
Bsp13I	TCCGGA	1 cut(s)	1837
Bsp1407I	TGTACA	1 cut(s)	2812
Bsp143I	GATC	9 cut(s)	88, 343, 679, 1582, 1756, 2233, 2401, 2438, 2900
Bsp1720I	GCTNAGC	2 cut(s)	33, 216
Bsp19I	CCATGG	4 cut(s)	39, 193, 1896, 2217
BspACI	CCGC	6 cut(s)	299, 833, 1112, 1761, 2636, 2820
BspANI	GGCC	9 cut(s)	69, 176, 470, 1543, 1752, 1940, 2120, 2735, 2800
BspEI	TCCGGA	1 cut(s)	1837
BspFNI	CGCG	1 cut(s)	2638
BspLI	GGNNCC	6 cut(s)	45, 681, 1105, 1480, 1758, 1941
BspMAI	CTGCAG	1 cut(s)	1249
BspMI	ACCTGC	2 cut(s)	2512, 2639
BspPI	GGATC	6 cut(s)	338, 674, 687, 1751, 1764, 2908
BspQI	GCTCTTC	1 cut(s)	1122
BspT104I	TTCGAA	2 cut(s)	339, 391
BspT107I	GGYRCC	1 cut(s)	1103
BsrDI	GCAATG	5 cut(s)	1245, 1335, 1417, 1784, 2233
BsrGI	TGTACA	1 cut(s)	2812
BsrI	ACTGG	4 cut(s)	98, 471, 2528, 2964
BssECI	CCNNGG	9 cut(s)	39, 193, 629, 1551, 1861, 1896, 2217, 2280, 2304
BssMI	GATC	9 cut(s)	88, 343, 679, 1582, 1756, 2233, 2401, 2438, 2900
BssT1I	CCWWGG	7 cut(s)	39, 193, 629, 1551, 1896, 2217, 2280
Bst2UI	CCWGG	3 cut(s)	1754, 1863, 2306
Bst4CI	ACNGT	8 cut(s)	293, 317, 589, 741, 1260, 2249, 2277, 2936
Bst6I	CTCTTC	7 cut(s)	552, 985, 1034, 1122, 1275, 2079, 2839
BstAPI	GCANNNNNTGC	3 cut(s)	226, 1244, 1430
BstAUI	TGTACA	1 cut(s)	2812
BstBI	TTCGAA	2 cut(s)	339, 391
BstC8I	GCNNGC	7 cut(s)	279, 757, 761, 1590, 2664, 2729, 2733
BstDSI	CCRYGG	4 cut(s)	39, 193, 1896, 2217
BstEII	GGTNACC	1 cut(s)	2675
BstF5I	GGATG	9 cut(s)	384, 480, 960, 1435, 1482, 2130, 2586, 2602, 2653
BstFNI	CGCG	1 cut(s)	2638
BstHHI	GCGC	2 cut(s)	2001, 2061
BstKTI	GATC	9 cut(s)	91, 346, 682, 1585, 1759, 2236, 2404, 2441, 2903
BstMAI	GTCTC	3 cut(s)	44, 2514, 2850
BstMBI	GATC	9 cut(s)	88, 343, 679, 1582, 1756, 2233, 2401, 2438, 2900
BstNI	CCWGG	3 cut(s)	1754, 1863, 2306
BstNSI	RCATGY	3 cut(s)	759, 1517, 2618
BstPAI	GACNNNNGTC	1 cut(s)	2526
BstPI	GGTNACC	1 cut(s)	2675
BstSCI	CCNGG	5 cut(s)	1752, 1861, 2304, 2484, 2795
BstSFI	CTRYAG	5 cut(s)	1245, 1811, 1956, 2154, 2193
BstSLI	GKGCMC	1 cut(s)	1108
BstUI	CGCG	1 cut(s)	2638
BstX2I	RGATCY	3 cut(s)	343, 679, 1756
BstYI	RGATCY	3 cut(s)	343, 679, 1756
BsuRI	GGCC	9 cut(s)	69, 176, 470, 1543, 1752, 1940, 2120, 2735, 2800
BtgI	CCRYGG	4 cut(s)	39, 193, 1896, 2217
BtgZI	GCGATG	1 cut(s)	839
BtsCI	GGATG	9 cut(s)	384, 480, 960, 1435, 1482, 2130, 2586, 2602, 2653
BtsI	GCAGTG	2 cut(s)	710, 906
BtsIMutI	CAGTG	6 cut(s)	91, 162, 464, 710, 906, 2273
BveI	ACCTGC	2 cut(s)	2512, 2639
Cac8I	GCNNGC	7 cut(s)	279, 757, 761, 1590, 2664, 2729, 2733
CaiI	CAGNNNCTG	1 cut(s)	32
CfoI	GCGC	2 cut(s)	2001, 2061
Cfr13I	GGNCC	5 cut(s)	96, 469, 1939, 2734, 2798
CspCI	CAANNNNNGTGG	2 cut(s)	2262, 2297
DpnI	GATC	9 cut(s)	90, 345, 681, 1584, 1758, 2235, 2403, 2440, 2902
DpnII	GATC	9 cut(s)	88, 343, 679, 1582, 1756, 2233, 2401, 2438, 2900
DraI	TTTAAA	1 cut(s)	370
EaeI	YGGCCR	3 cut(s)	67, 174, 1750
Eam1104I	CTCTTC	7 cut(s)	552, 985, 1034, 1122, 1275, 2079, 2839
EarI	CTCTTC	7 cut(s)	552, 985, 1034, 1122, 1275, 2079, 2839
Ecl136II	GAGCTC	2 cut(s)	272, 1696
Eco130I	CCWWGG	7 cut(s)	39, 193, 629, 1551, 1896, 2217, 2280
Eco24I	GRGCYC	3 cut(s)	39, 274, 1698
Eco47I	GGWCC	1 cut(s)	96
Eco53kI	GAGCTC	2 cut(s)	272, 1696
Eco57I	CTGAAG	3 cut(s)	2199, 2373, 2664
Eco88I	CYCGRG	2 cut(s)	944, 2040
Eco91I	GGTNACC	1 cut(s)	2675
EcoICRI	GAGCTC	2 cut(s)	272, 1696
EcoO109I	RGGNCCY	1 cut(s)	1939
EcoO65I	GGTNACC	1 cut(s)	2675
EcoRI	GAATTC	2 cut(s)	121, 2299
EcoRII	CCWGG	3 cut(s)	1752, 1861, 2304
EcoT14I	CCWWGG	7 cut(s)	39, 193, 629, 1551, 1896, 2217, 2280
EcoT38I	GRGCYC	3 cut(s)	39, 274, 1698
ErhI	CCWWGG	7 cut(s)	39, 193, 629, 1551, 1896, 2217, 2280
FalI	AAGNNNNNCTT	2 cut(s)	790, 822
FaqI	GGGAC	4 cut(s)	434, 611, 1843, 2945
FauI	CCCGC	3 cut(s)	826, 1768, 2643
FbaI	TGATCA	2 cut(s)	1582, 2401
FokI	GGATG	9 cut(s)	391, 467, 967, 1442, 1469, 2117, 2589, 2593, 2640
FriOI	GRGCYC	3 cut(s)	39, 274, 1698
FspBI	CTAG	5 cut(s)	630, 1235, 1620, 1665, 2393
GlaI	GCGC	2 cut(s)	2000, 2060
GsaI	CCCAGC	1 cut(s)	2740
GsuI	CTGGAG	3 cut(s)	39, 2346, 2545
HaeIII	GGCC	9 cut(s)	69, 176, 470, 1543, 1752, 1940, 2120, 2735, 2800
HapII	CCGG	3 cut(s)	1838, 2486, 2796
HhaI	GCGC	2 cut(s)	2001, 2061
Hin6I	GCGC	2 cut(s)	1999, 2059
HinP1I	GCGC	2 cut(s)	1999, 2059
HincII	GTYRAC	1 cut(s)	1468
HindII	GTYRAC	1 cut(s)	1468
HindIII	AAGCTT	4 cut(s)	606, 905, 1447, 2355
HpaII	CCGG	3 cut(s)	1838, 2486, 2796
HphI	GGTGA	3 cut(s)	632, 1063, 2669
Hpy166II	GTNNAC	5 cut(s)	310, 464, 1468, 1506, 2258
Hpy8I	GTNNAC	5 cut(s)	310, 464, 1468, 1506, 2258
HpyAV	CCTTC	7 cut(s)	357, 683, 693, 1539, 2174, 2706, 2980
HpyCH4III	ACNGT	8 cut(s)	293, 317, 589, 741, 1260, 2249, 2277, 2936
HpyCH4IV	ACGT	1 cut(s)	1189
HpySE526I	ACGT	1 cut(s)	1189
HspAI	GCGC	2 cut(s)	1999, 2059
Kpn2I	TCCGGA	1 cut(s)	1837
Ksp22I	TGATCA	2 cut(s)	1582, 2401
Kzo9I	GATC	9 cut(s)	88, 343, 679, 1582, 1756, 2233, 2401, 2438, 2900
LguI	GCTCTTC	1 cut(s)	1122
LmnI	GCTCC	8 cut(s)	242, 823, 1081, 1478, 1701, 1996, 2056, 2875
MaeI	CTAG	5 cut(s)	630, 1235, 1620, 1665, 2393
MaeII	ACGT	1 cut(s)	1189
MaeIII	GTNAC	4 cut(s)	1375, 2243, 2586, 2675
MalI	GATC	9 cut(s)	90, 345, 681, 1584, 1758, 2235, 2403, 2440, 2902
MboI	GATC	9 cut(s)	88, 343, 679, 1582, 1756, 2233, 2401, 2438, 2900
MfeI	CAATTG	1 cut(s)	687
MflI	RGATCY	3 cut(s)	343, 679, 1756
MhlI	GDGCHC	5 cut(s)	39, 239, 274, 1108, 1698
MlsI	TGGCCA	1 cut(s)	1752
MluNI	TGGCCA	1 cut(s)	1752
MlyI	GAGTC	8 cut(s)	46, 1145, 1361, 1501, 1676, 2296, 2402, 2536
MmeI	TCCRAC	2 cut(s)	822, 2884
Mox20I	TGGCCA	1 cut(s)	1752
MroI	TCCGGA	1 cut(s)	1837
MroXI	GAANNNNTTC	1 cut(s)	2188
MscI	TGGCCA	1 cut(s)	1752
MseI	TTAA	7 cut(s)	369, 792, 1077, 1155, 1169, 1572, 2535
MslI	CAYNNNNRTG	2 cut(s)	1356, 1358
Msp20I	TGGCCA	1 cut(s)	1752
MspA1I	CMGCKG	2 cut(s)	301, 2453
MspI	CCGG	3 cut(s)	1838, 2486, 2796
MspR9I	CCNGG	5 cut(s)	1754, 1863, 2306, 2486, 2797
MunI	CAATTG	1 cut(s)	687
Mva1269I	GAATGC	2 cut(s)	1072, 1426
MvaI	CCWGG	3 cut(s)	1754, 1863, 2306
MvnI	CGCG	1 cut(s)	2638
NciI	CCSGG	2 cut(s)	2486, 2797
NcoI	CCATGG	4 cut(s)	39, 193, 1896, 2217
NdeII	GATC	9 cut(s)	88, 343, 679, 1582, 1756, 2233, 2401, 2438, 2900
NlaIV	GGNNCC	6 cut(s)	45, 681, 1105, 1480, 1758, 1941
NmeAIII	GCCGAG	3 cut(s)	95, 202, 1489
NmuCI	GTSAC	2 cut(s)	2243, 2675
NspI	RCATGY	3 cut(s)	759, 1517, 2618
NspV	TTCGAA	2 cut(s)	339, 391
PaeI	GCATGC	1 cut(s)	759
PaqCI	CACCTGC	1 cut(s)	2512
PciSI	GCTCTTC	1 cut(s)	1122
PcsI	WCGNNNNNNNCGW	1 cut(s)	1516
PctI	GAATGC	2 cut(s)	1072, 1426
PdmI	GAANNNNTTC	1 cut(s)	2188
PflMI	CCANNNNNTGG	1 cut(s)	689
PleI	GAGTC	8 cut(s)	46, 1144, 1361, 1501, 1676, 2295, 2402, 2535
PpsI	GAGTC	8 cut(s)	46, 1144, 1361, 1501, 1676, 2295, 2402, 2535
PshAI	GACNNNNGTC	1 cut(s)	2526
PshBI	ATTAAT	2 cut(s)	792, 2535
Psp124BI	GAGCTC	2 cut(s)	274, 1698
Psp1406I	AACGTT	1 cut(s)	1189
Psp6I	CCWGG	3 cut(s)	1752, 1861, 2304
PspEI	GGTNACC	1 cut(s)	2675
PspFI	CCCAGC	1 cut(s)	2736
PspGI	CCWGG	3 cut(s)	1752, 1861, 2304
PspN4I	GGNNCC	6 cut(s)	45, 681, 1105, 1480, 1758, 1941
PspPI	GGNCC	5 cut(s)	96, 469, 1939, 2734, 2798
PstI	CTGCAG	1 cut(s)	1249
PstNI	CAGNNNCTG	1 cut(s)	32
PsuI	RGATCY	3 cut(s)	343, 679, 1756
PvuII	CAGCTG	1 cut(s)	2453
RseI	CAYNNNNRTG	2 cut(s)	1356, 1358
SacI	GAGCTC	2 cut(s)	274, 1698
SapI	GCTCTTC	1 cut(s)	1122
SaqAI	TTAA	7 cut(s)	369, 792, 1077, 1155, 1169, 1572, 2535
Sau3AI	GATC	9 cut(s)	88, 343, 679, 1582, 1756, 2233, 2401, 2438, 2900
Sau96I	GGNCC	5 cut(s)	96, 469, 1939, 2734, 2798
ScaI	AGTACT	1 cut(s)	443
SchI	GAGTC	8 cut(s)	46, 1145, 1361, 1501, 1676, 2296, 2402, 2536
ScrFI	CCNGG	5 cut(s)	1754, 1863, 2306, 2486, 2797
SduI	GDGCHC	5 cut(s)	39, 239, 274, 1108, 1698
SfcI	CTRYAG	5 cut(s)	1245, 1811, 1956, 2154, 2193
SfuI	TTCGAA	2 cut(s)	339, 391
SinI	GGWCC	1 cut(s)	96
SmiMI	CAYNNNNRTG	2 cut(s)	1356, 1358
SphI	GCATGC	1 cut(s)	759
SsiI	CCGC	6 cut(s)	299, 833, 1112, 1761, 2636, 2820
SspI	AATATT	1 cut(s)	1032
SspMI	CTAG	5 cut(s)	630, 1235, 1620, 1665, 2393
SstI	GAGCTC	2 cut(s)	274, 1698
StyD4I	CCNGG	5 cut(s)	1752, 1861, 2304, 2484, 2795
StyI	CCWWGG	7 cut(s)	39, 193, 629, 1551, 1896, 2217, 2280
TaaI	ACNGT	8 cut(s)	293, 317, 589, 741, 1260, 2249, 2277, 2936
TaiI	ACGT	1 cut(s)	1192
TaqI	TCGA	4 cut(s)	87, 339, 391, 2236
TaqII	GACCGA	1 cut(s)	1032
TatI	WGTACW	5 cut(s)	441, 1096, 1673, 2372, 2812
TauI	GCSGC	2 cut(s)	1115, 2822
Tru1I	TTAA	7 cut(s)	369, 792, 1077, 1155, 1169, 1572, 2535
Tru9I	TTAA	7 cut(s)	369, 792, 1077, 1155, 1169, 1572, 2535
TscAI	CASTG	6 cut(s)	98, 162, 471, 717, 906, 2280
TseFI	GTSAC	2 cut(s)	2243, 2675
Tsp45I	GTSAC	2 cut(s)	2243, 2675
TspGWI	ACGGA	1 cut(s)	174
TspRI	CASTG	6 cut(s)	98, 162, 471, 717, 906, 2280
Van91I	CCANNNNNTGG	1 cut(s)	689
VpaK11BI	GGWCC	1 cut(s)	96
VspI	ATTAAT	2 cut(s)	792, 2535
XbaI	TCTAGA	1 cut(s)	1664
XceI	RCATGY	3 cut(s)	759, 1517, 2618
XmaJI	CCTAGG	1 cut(s)	629
XmnI	GAANNNNTTC	1 cut(s)	2188
XspI	CTAG	5 cut(s)	630, 1235, 1620, 1665, 2393
ZrmI	AGTACT	1 cut(s)	443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.