RchiOBHm_Chr5g0078281

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005300
Physical Location & Seq
Forward (+)
84196507 .. 84197322
816 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35281

Sequence Viewer

Length: 618 bp
ATGAGACTCCAGAGTCCCCAAAAACCTGCTCTCACCTTCTTCCACAGCGAAGTCAAAAACCATCTGACCTGTGGAAGCCAAAATCTCGCTCTTCGACTCTTCACTTCAGCTTCAGTGAGAAAACCACCTGCTACTGTTCGCTTGGGTCTCCTTCCATCTCTTCTGCAGGTTCAATTTTTCACTCTCCCCTCCAATCCCCTCAGTCAAAATGCTAAACAGAAAAATACATTATTCGGGACAAATATTCACACCGTGGCAGCACGGCAGCATGGCCTCACGCGCCGCCGCCGGGAGATGGCCTGGGAGCAGCTCCACTCCGGCCCCTGGCACTCCGTCCTCCCCGTCTGGCGCGACGCCGACGCAATGGCCTGCCTCCACGTCGCCAAGCTCCACTTCGCCGCAGCTGAGTTCACCGACGCGCTCCGCGCCCTCGACATGGGCCTCCTCATGGGCGGCCCGATTTTTAAATCGGACCTCCACTCCGCCATGACTAAAGTCCCCGCTAAATCCAGAGCCGCCGCTAGGGTTCCCGAACAAAACGGCCCCAATTTGGACCGCCGGTTGGTCCGTGACGACCTCAAAGCAACGGAGATGCTTGAATTTTGCCTCCAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000086 GO:0000278 GO:0003674 GO:0003682 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006807 GO:0006996 GO:0007049 GO:0007623 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008214 GO:0008276 GO:0008757 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016278 GO:0016279 GO:0016491 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016705 GO:0016706 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032259 GO:0032451 GO:0032452 GO:0034968 GO:0036211 GO:0042054 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0045893 GO:0045935 GO:0046975 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051213 GO:0051252 GO:0051254 GO:0051276 GO:0051864 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070544 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:0140110 GO:1901564 GO:1902680 GO:1903047 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_8

Protein Analysis

205

Amino Acids

22.91

Weight (kDa)

10.33

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_KDM8_N PF24472 95 - 177 1.8e-39 KDM8-like, N-terminal ARM repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 136
AasI GACNNNNNNGTC 1 cut(s) 12
Acc36I ACCTGC 3 cut(s) 34, 136, 157
AccII CGCG 4 cut(s) 280, 351, 419, 426
AcsI RAATTY 1 cut(s) 599
AcuI CTGAAG 2 cut(s) 90, 96
AcyI GRCGYC 1 cut(s) 354
AdeI CACNNNGTG 1 cut(s) 253
AfiI CCNNNNNNNGG 8 cut(s) 289, 295, 324, 436, 448, 522, 550, 562
AgsI TTSAA 2 cut(s) 173, 599
AjiI CACGTC 1 cut(s) 379
AjnI CCWGG 2 cut(s) 299, 323
AjuI GAANNNNNNNTTGG 2 cut(s) 377, 409
AluBI AGCT 4 cut(s) 110, 310, 388, 404
AluI AGCT 4 cut(s) 110, 310, 388, 404
Alw26I GTCTC 1 cut(s) 152
AoxI GGCC 7 cut(s) 271, 297, 319, 366, 439, 454, 541
ApeKI GCWGC 4 cut(s) 257, 265, 307, 401
ApoI RAATTY 1 cut(s) 599
AspLEI GCGC 4 cut(s) 282, 351, 421, 428
AspS9I GGNCC 7 cut(s) 320, 439, 455, 472, 542, 553, 565
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 2 cut(s) 25, 403
AvaII GGWCC 3 cut(s) 472, 553, 565
BbvI GCAGC 4 cut(s) 269, 277, 319, 413
BccI CCATC 3 cut(s) 69, 163, 289
BceAI ACGGC 2 cut(s) 278, 556
BciT130I CCWGG 2 cut(s) 301, 325
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 164
BfuAI ACCTGC 3 cut(s) 34, 136, 157
Bme1390I CCNGG 3 cut(s) 290, 301, 325
Bme18I GGWCC 3 cut(s) 472, 553, 565
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 7 cut(s) 320, 439, 455, 472, 542, 553, 565
BmiI GGNNCC 3 cut(s) 322, 528, 544
BmrFI CCNGG 3 cut(s) 290, 301, 325
BmsI GCATC 1 cut(s) 582
BoxI GACNNNNGTC 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 252, 300, 323
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 8 cut(s) 289, 295, 324, 436, 448, 522, 550, 562
Bse118I RCCGGY 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 2 cut(s) 301, 325
BseDI CCNNGG 3 cut(s) 252, 300, 323
BseLI CCNNNNNNNGG 8 cut(s) 289, 295, 324, 436, 448, 522, 550, 562
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 214, 396
BseRI GAGGAG 1 cut(s) 434
BseXI GCAGC 4 cut(s) 269, 277, 319, 413
Bsh1236I CGCG 4 cut(s) 280, 351, 419, 426
BshFI GGCC 7 cut(s) 273, 299, 321, 368, 441, 456, 543
BsiSI CCGG 3 cut(s) 289, 318, 559
BslFI GGGAC 2 cut(s) 250, 482
BslI CCNNNNNNNGG 8 cut(s) 289, 295, 324, 436, 448, 522, 550, 562
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 250, 482
BsnI GGCC 7 cut(s) 273, 299, 321, 368, 441, 456, 543
Bso31I GGTCTC 1 cut(s) 152
BspANI GGCC 7 cut(s) 273, 299, 321, 368, 441, 456, 543
BspCNI CTCAG 2 cut(s) 213, 397
BspFNI CGCG 4 cut(s) 280, 351, 419, 426
BspLI GGNNCC 3 cut(s) 322, 528, 544
BspMAI CTGCAG 1 cut(s) 168
BspMI ACCTGC 3 cut(s) 34, 136, 157
BspQI GCTCTTC 1 cut(s) 96
BspTNI GGTCTC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 558
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 3 cut(s) 252, 300, 323
BssNI GRCGYC 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 301, 325
Bst4CI ACNGT 2 cut(s) 136, 253
Bst6I CTCTTC 3 cut(s) 96, 104, 165
BstACI GRCGYC 1 cut(s) 354
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 2 cut(s) 200, 405
BstDSI CCRYGG 1 cut(s) 252
BstFNI CGCG 4 cut(s) 280, 351, 419, 426
BstHHI GCGC 4 cut(s) 282, 351, 421, 428
BstMAI GTCTC 1 cut(s) 152
BstMWI GCNNNNNNNGC 2 cut(s) 279, 425
BstNI CCWGG 2 cut(s) 301, 325
BstPAI GACNNNNGTC 1 cut(s) 494
BstSCI CCNGG 3 cut(s) 288, 299, 323
BstSFI CTRYAG 1 cut(s) 164
BstUI CGCG 4 cut(s) 280, 351, 419, 426
BstV1I GCAGC 4 cut(s) 269, 277, 319, 413
BsuRI GGCC 7 cut(s) 273, 299, 321, 368, 441, 456, 543
BtgI CCRYGG 1 cut(s) 252
BtrI CACGTC 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 120
BveI ACCTGC 3 cut(s) 34, 136, 157
Cac8I GCNNGC 1 cut(s) 370
CfoI GCGC 4 cut(s) 282, 351, 421, 428
Cfr10I RCCGGY 1 cut(s) 558
Cfr13I GGNCC 7 cut(s) 320, 439, 455, 472, 542, 553, 565
CseI GACGC 3 cut(s) 362, 368, 425
CviAII CATG 4 cut(s) 269, 436, 448, 487
DdeI CTNAG 2 cut(s) 200, 405
DraI TTTAAA 1 cut(s) 466
DraIII CACNNNGTG 1 cut(s) 253
DrdI GACNNNNNNGTC 1 cut(s) 12
DseDI GACNNNNNNGTC 1 cut(s) 12
Eam1104I CTCTTC 3 cut(s) 96, 104, 165
EarI CTCTTC 3 cut(s) 96, 104, 165
EciI GGCGGA 1 cut(s) 472
Eco31I GGTCTC 1 cut(s) 152
Eco47I GGWCC 3 cut(s) 472, 553, 565
Eco57I CTGAAG 2 cut(s) 90, 96
EcoRII CCWGG 2 cut(s) 299, 323
FaeI CATG 4 cut(s) 272, 439, 451, 490
FaiI YATR 4 cut(s) 270, 437, 449, 488
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 2 cut(s) 250, 482
FatI CATG 4 cut(s) 268, 435, 447, 486
FauI CCCGC 1 cut(s) 508
FspBI CTAG 1 cut(s) 522
GlaI GCGC 4 cut(s) 281, 350, 420, 427
HaeIII GGCC 7 cut(s) 273, 299, 321, 368, 441, 456, 543
HapII CCGG 3 cut(s) 289, 318, 559
HgaI GACGC 3 cut(s) 362, 368, 425
HhaI GCGC 4 cut(s) 282, 351, 421, 428
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 4 cut(s) 272, 439, 451, 490
Hin6I GCGC 4 cut(s) 280, 349, 419, 426
HinP1I GCGC 4 cut(s) 280, 349, 419, 426
HinfI GANTC 3 cut(s) 6, 13, 96
HpaII CCGG 3 cut(s) 289, 318, 559
HphI GGTGA 2 cut(s) 25, 403
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 2 cut(s) 66, 472
Hpy188III TCNNGA 4 cut(s) 10, 235, 510, 530
Hpy8I GTNNAC 1 cut(s) 411
Hpy99I CGWCG 4 cut(s) 356, 362, 383, 419
HpyAV CCTTC 2 cut(s) 46, 161
HpyCH4III ACNGT 2 cut(s) 136, 253
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 1 cut(s) 166
HpyF10VI GCNNNNNNNGC 2 cut(s) 279, 425
HpyF3I CTNAG 2 cut(s) 200, 405
HpySE526I ACGT 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 4 cut(s) 272, 439, 451, 490
HspAI GCGC 4 cut(s) 280, 349, 419, 426
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 4 cut(s) 304, 315, 393, 426
Lsp1109I GCAGC 4 cut(s) 269, 277, 319, 413
LweI GCATC 1 cut(s) 582
MaeI CTAG 1 cut(s) 522
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 569
MboII GAAGA 4 cut(s) 31, 83, 91, 152
MluCI AATT 4 cut(s) 173, 547, 599, 613
MlyI GAGTC 2 cut(s) 22, 90
MseI TTAA 1 cut(s) 465
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 3 cut(s) 289, 318, 559
MspR9I CCNGG 3 cut(s) 290, 301, 325
MvaI CCWGG 2 cut(s) 301, 325
MvnI CGCG 4 cut(s) 280, 351, 419, 426
MwoI GCNNNNNNNGC 2 cut(s) 279, 425
NciI CCSGG 1 cut(s) 290
NlaIII CATG 4 cut(s) 272, 439, 451, 490
NlaIV GGNNCC 3 cut(s) 322, 528, 544
NmuCI GTSAC 1 cut(s) 569
PaqCI CACCTGC 1 cut(s) 136
PciSI GCTCTTC 1 cut(s) 96
PleI GAGTC 2 cut(s) 21, 90
PpsI GAGTC 2 cut(s) 21, 90
PshAI GACNNNNGTC 1 cut(s) 494
Psp6I CCWGG 2 cut(s) 299, 323
PspGI CCWGG 2 cut(s) 299, 323
PspN4I GGNNCC 3 cut(s) 322, 528, 544
PspPI GGNCC 7 cut(s) 320, 439, 455, 472, 542, 553, 565
PstI CTGCAG 1 cut(s) 168
PvuII CAGCTG 1 cut(s) 404
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 465
Sau96I GGNCC 7 cut(s) 320, 439, 455, 472, 542, 553, 565
SchI GAGTC 2 cut(s) 22, 90
ScrFI CCNGG 3 cut(s) 290, 301, 325
SfaNI GCATC 1 cut(s) 582
SfcI CTRYAG 1 cut(s) 164
SinI GGWCC 3 cut(s) 472, 553, 565
Sse9I AATT 4 cut(s) 173, 547, 599, 613
SspI AATATT 1 cut(s) 244
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 3 cut(s) 288, 299, 323
TaaI ACNGT 2 cut(s) 136, 253
TaiI ACGT 1 cut(s) 381
TaqI TCGA 2 cut(s) 94, 432
TasI AATT 4 cut(s) 173, 547, 599, 613
TauI GCSGC 6 cut(s) 285, 288, 401, 456, 518, 521
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 1 cut(s) 569
TseI GCWGC 4 cut(s) 257, 265, 307, 401
Tsp45I GTSAC 1 cut(s) 569
TspGWI ACGGA 3 cut(s) 322, 557, 602
TspRI CASTG 1 cut(s) 120
VpaK11BI GGWCC 3 cut(s) 472, 553, 565
XapI RAATTY 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 68
XspI CTAG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.