Rw5G047680

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005300
Physical Location & Seq
Forward (+)
82575120 .. 82578977
3858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047680.1

Sequence Viewer

Length: 1200 bp
ATGGCCAACTCCGACCACCACCACCACCACCACCACCACCACCACCACCACCTCCCCACCCCGACCCTCGACTCCGAGTCCTCCACGCTCCTCCACGCCCTCTCCGAGCACGGCGGCTACGCCTACGTCAGCATGGCCACACGCGCCGCCACCGGCGACGTCCGGGCCGCCGAGGCCGCTCGCGAGATGGCCTGGGAGCAGCTCCACTCCGGCCCCTGGCACTCCGTCCTCCCCGTCTGGCGCGACGCCTACGCAATGGCCTGCCTCCACGTCGCCAAGCTCCACTTCGCCGCCGCTGAGTTCACCGACGCCCTCCGCGCCCTCGACATGGGCCTCCTCATGGGCGGCCCGCTCTTCAAATCGGACCTTCACTCCGCCGTGACTAAAGTCTCCGCTAAATCCAGAGCCGCCGCCGCTAGGGTTTCCGAACAAAACGGCCCCAATTTGGACCGCCGTCTGGTCCGTGACGACCCCAAAGCAACGGAGATGCTTGGGGTTTTGCCTCCAAAATCACTATCTTGTAAAACGGTTGTGAAGAAGTCTGGTTTGTCTTTGGAAGGGTTTTTAAGAGAACATTTCTTACCGGGATTGCCGGTTATTTTGACCGATTGTATGGCACATTGGCCGGCGAGGACAAAGTGGAATGACATGGATTACCTGATAAGAGTTGCCGGTGACCGGACAGTGCCGGTTGAGGTTGGGAAAAATTACTTGTATCCGGAGTGGAAGCAGGAGCTTATTACGTTTTCGCAGTTTCTTGAGAGGATTCGGGGTAATGATTCTTCATCTGGTGCGCCGACATATCTTGCTCAGCACTCATTGTTTGATCAGATAAACGAGCTGCGTGAGGACATATGTATTCCCGACTATTGTTGTGCTGGTGGTGGGGAACTAAAGTCTCTAAATGCTTGGTTTGGTCCGGCTGGGACTGTAACCCCATTACACCATGATCCGCACCATAATGTGTTTGCACAGGTAGTTGGAAAAAAGTATATAAGGCTCTATCGTGCTTCATTGTCAGAGGAACTCTATCCACATACTGAAACCATGCTTTGCAACTCAAGTCAGGTTGATCTTGACAATGTAGATGAGAAAGAATTCCCGGAGGTGCAGGACTTGGAATTTCTTGACTGCATATTAGAGGAAGGTGACATGCTTTATATCCCACCAAAGTGGTGGCACTATGTGCGATGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000086 GO:0000278 GO:0003674 GO:0003682 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006807 GO:0006996 GO:0007049 GO:0007623 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008214 GO:0008276 GO:0008757 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016278 GO:0016279 GO:0016491 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016705 GO:0016706 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032259 GO:0032451 GO:0032452 GO:0034968 GO:0036211 GO:0042054 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0045893 GO:0045935 GO:0046975 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051213 GO:0051252 GO:0051254 GO:0051276 GO:0051864 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070544 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:0140110 GO:1901564 GO:1902680 GO:1903047 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_8

Protein Analysis

399

Amino Acids

45.02

Weight (kDa)

6.03

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_KDM8_N PF24472 20 - 142 9.9e-63 KDM8-like, N-terminal ARM repeats
Cupin_8 PF13621 188 - 397 1.2e-43 Cupin-like domain
JmjC_2 PF08007 302 - 396 2.1e-09 JmjC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 162
AccBSI CCGCTC 2 cut(s) 179, 352
AccII CGCG 4 cut(s) 144, 183, 243, 318
AccIII TCCGGA 1 cut(s) 718
AclWI GGATC 1 cut(s) 944
AcoI YGGCCR 3 cut(s) 3, 135, 623
AcsI RAATTY 2 cut(s) 1097, 1121
AcyI GRCGYC 3 cut(s) 159, 246, 309
AdeI CACNNNGTG 1 cut(s) 1186
AfiI CCNNNNNNNGG 7 cut(s) 216, 328, 340, 417, 445, 457, 678
AgsI TTSAA 1 cut(s) 358
AhdI GACNNNNNGTC 1 cut(s) 76
AjiI CACGTC 1 cut(s) 271
AjnI CCWGG 2 cut(s) 191, 215
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AleI CACNNNNGTG 1 cut(s) 1171
AluBI AGCT 4 cut(s) 202, 280, 736, 841
AluI AGCT 4 cut(s) 202, 280, 736, 841
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 2 cut(s) 394, 903
AlwI GGATC 1 cut(s) 944
Aor13HI TCCGGA 1 cut(s) 718
ApeKI GCWGC 2 cut(s) 199, 841
ApoI RAATTY 2 cut(s) 1097, 1121
Asp700I GAANNNNTTC 1 cut(s) 1097
AspLEI GCGC 4 cut(s) 146, 243, 320, 796
AspS9I GGNCC 9 cut(s) 165, 212, 331, 347, 364, 437, 448, 460, 917
AsuC2I CCSGG 3 cut(s) 164, 585, 1103
AsuHPI GGTGA 3 cut(s) 295, 686, 1160
AvaII GGWCC 4 cut(s) 364, 448, 460, 917
BalI TGGCCA 2 cut(s) 5, 137
BarI GAAGNNNNNNTAC 2 cut(s) 766, 798
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 211, 828
BccI CCATC 2 cut(s) 181, 1185
BceAI ACGGC 4 cut(s) 127, 362, 438, 451
BciT130I CCWGG 2 cut(s) 193, 217
BciVI GTATCC 1 cut(s) 726
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 3 cut(s) 164, 585, 1103
BcoDI GTCTC 2 cut(s) 394, 903
BfaI CTAG 1 cut(s) 417
BfuI GTATCC 1 cut(s) 726
BglI GCCNNNNNGGC 1 cut(s) 173
BlpI GCTNAGC 1 cut(s) 810
Bme1390I CCNGG 5 cut(s) 164, 193, 217, 585, 1103
Bme18I GGWCC 4 cut(s) 364, 448, 460, 917
BmeRI GACNNNNNGTC 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 9 cut(s) 165, 212, 331, 347, 364, 437, 448, 460, 917
BmiI GGNNCC 2 cut(s) 214, 439
BmrFI CCNGG 5 cut(s) 164, 193, 217, 585, 1103
BmsI GCATC 1 cut(s) 477
BoxI GACNNNNGTC 2 cut(s) 386, 453
Bpu1102I GCTNAGC 1 cut(s) 810
BpuEI CTTGAG 2 cut(s) 779, 1045
BpuMI CCSGG 3 cut(s) 164, 585, 1103
BsaHI GRCGYC 3 cut(s) 159, 246, 309
BsaJI CCNNGG 3 cut(s) 171, 192, 215
BsaWI WCCGGW 2 cut(s) 678, 718
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 356, 386
Bsc4I CCNNNNNNNGG 7 cut(s) 216, 328, 340, 417, 445, 457, 678
Bse118I RCCGGY 5 cut(s) 152, 592, 625, 671, 688
Bse3DI GCAATG 1 cut(s) 261
BseAI TCCGGA 1 cut(s) 718
BseBI CCWGG 2 cut(s) 193, 217
BseDI CCNNGG 3 cut(s) 171, 192, 215
BseLI CCNNNNNNNGG 7 cut(s) 216, 328, 340, 417, 445, 457, 678
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 288, 824
BseRI GAGGAG 2 cut(s) 80, 326
BseXI GCAGC 2 cut(s) 211, 828
BseYI CCCAGC 1 cut(s) 923
BsgI GTGCAG 1 cut(s) 1130
Bsh1236I CGCG 4 cut(s) 144, 183, 243, 318
BsiHKAI GWGCWC 1 cut(s) 111
BslFI GGGAC 1 cut(s) 940
BslI CCNNNNNNNGG 7 cut(s) 216, 328, 340, 417, 445, 457, 678
BsmAI GTCTC 2 cut(s) 394, 903
BsmFI GGGAC 1 cut(s) 940
Bsp1286I GDGCHC 1 cut(s) 111
Bsp13I TCCGGA 1 cut(s) 718
Bsp143I GATC 3 cut(s) 826, 949, 1072
Bsp1720I GCTNAGC 1 cut(s) 810
Bsp68I TCGCGA 1 cut(s) 183
BspCNI CTCAG 2 cut(s) 289, 823
BspEI TCCGGA 1 cut(s) 718
BspFNI CGCG 4 cut(s) 144, 183, 243, 318
BspLI GGNNCC 2 cut(s) 214, 439
BspPI GGATC 1 cut(s) 944
BspQI GCTCTTC 1 cut(s) 359
BsrBI CCGCTC 2 cut(s) 179, 352
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 5 cut(s) 152, 592, 625, 671, 688
BssAI RCCGGY 5 cut(s) 152, 592, 625, 671, 688
BssECI CCNNGG 3 cut(s) 171, 192, 215
BssMI GATC 3 cut(s) 826, 949, 1072
BssNI GRCGYC 3 cut(s) 159, 246, 309
Bst2UI CCWGG 2 cut(s) 193, 217
Bst4CI ACNGT 3 cut(s) 529, 685, 931
Bst6I CTCTTC 1 cut(s) 359
BstACI GRCGYC 3 cut(s) 159, 246, 309
BstAPI GCANNNNNTGC 1 cut(s) 1186
BstC8I GCNNGC 4 cut(s) 181, 262, 350, 627
BstDEI CTNAG 2 cut(s) 297, 810
BstEII GGTNACC 1 cut(s) 674
BstFNI CGCG 4 cut(s) 144, 183, 243, 318
BstHHI GCGC 4 cut(s) 146, 243, 320, 796
BstKTI GATC 3 cut(s) 829, 952, 1075
BstMAI GTCTC 2 cut(s) 394, 903
BstMBI GATC 3 cut(s) 826, 949, 1072
BstMWI GCNNNNNNNGC 6 cut(s) 143, 173, 176, 317, 413, 1186
BstNI CCWGG 2 cut(s) 193, 217
BstNSI RCATGY 1 cut(s) 1156
BstPAI GACNNNNGTC 2 cut(s) 386, 453
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 5 cut(s) 162, 191, 215, 583, 1101
BstUI CGCG 4 cut(s) 144, 183, 243, 318
BstV1I GCAGC 2 cut(s) 211, 828
BstXI CCANNNNNNTGG 2 cut(s) 1173, 1176
BsuI GTATCC 1 cut(s) 726
BtrI CACGTC 1 cut(s) 271
BtsIMutI CAGTG 1 cut(s) 690
BtuMI TCGCGA 1 cut(s) 183
Cac8I GCNNGC 4 cut(s) 181, 262, 350, 627
CfoI GCGC 4 cut(s) 146, 243, 320, 796
Cfr10I RCCGGY 5 cut(s) 152, 592, 625, 671, 688
Cfr13I GGNCC 9 cut(s) 165, 212, 331, 347, 364, 437, 448, 460, 917
CseI GACGC 2 cut(s) 254, 317
CviAII CATG 7 cut(s) 133, 328, 340, 649, 947, 1048, 1153
DdeI CTNAG 2 cut(s) 297, 810
DpnI GATC 3 cut(s) 828, 951, 1074
DpnII GATC 3 cut(s) 826, 949, 1072
DraIII CACNNNGTG 1 cut(s) 1186
DriI GACNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 3 cut(s) 3, 135, 623
Eam1104I CTCTTC 1 cut(s) 359
Eam1105I GACNNNNNGTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 359
EciI GGCGGA 1 cut(s) 364
Eco47I GGWCC 4 cut(s) 364, 448, 460, 917
Eco91I GGTNACC 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 674
EcoRI GAATTC 1 cut(s) 1097
EcoRII CCWGG 2 cut(s) 191, 215
FaeI CATG 7 cut(s) 136, 331, 343, 652, 950, 1051, 1156
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 940
FatI CATG 7 cut(s) 132, 327, 339, 648, 946, 1047, 1152
FauI CCCGC 1 cut(s) 357
FauNDI CATATG 1 cut(s) 854
FbaI TGATCA 1 cut(s) 826
FspBI CTAG 1 cut(s) 417
GlaI GCGC 4 cut(s) 145, 242, 319, 795
GsaI CCCAGC 1 cut(s) 927
HgaI GACGC 2 cut(s) 254, 317
HhaI GCGC 4 cut(s) 146, 243, 320, 796
Hin1I GRCGYC 3 cut(s) 159, 246, 309
Hin1II CATG 7 cut(s) 136, 331, 343, 652, 950, 1051, 1156
Hin6I GCGC 4 cut(s) 144, 241, 318, 794
HinP1I GCGC 4 cut(s) 144, 241, 318, 794
HinfI GANTC 4 cut(s) 71, 77, 766, 779
HphI GGTGA 3 cut(s) 295, 686, 1160
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 7 cut(s) 13, 76, 106, 364, 427, 831, 1021
Hpy188III TCNNGA 7 cut(s) 182, 402, 719, 758, 863, 1076, 1127
Hpy8I GTNNAC 1 cut(s) 303
Hpy99I CGWCG 4 cut(s) 161, 248, 275, 311
HpyAV CCTTC 3 cut(s) 377, 551, 1139
HpyCH4III ACNGT 3 cut(s) 529, 685, 931
HpyCH4IV ACGT 4 cut(s) 126, 159, 270, 743
HpyCH4V TGCA 4 cut(s) 971, 1056, 1111, 1134
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 173, 176, 317, 413, 1186
HpyF3I CTNAG 2 cut(s) 297, 810
HpySE526I ACGT 4 cut(s) 126, 159, 270, 743
Hsp92I GRCGYC 3 cut(s) 159, 246, 309
Hsp92II CATG 7 cut(s) 136, 331, 343, 652, 950, 1051, 1156
HspAI GCGC 4 cut(s) 144, 241, 318, 794
Kpn2I TCCGGA 1 cut(s) 718
KroI GCCGGC 1 cut(s) 625
KroNI GCCGGC 1 cut(s) 627
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 3 cut(s) 826, 949, 1072
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 5 cut(s) 93, 196, 207, 285, 733
Lsp1109I GCAGC 2 cut(s) 211, 828
LweI GCATC 1 cut(s) 477
MaeI CTAG 1 cut(s) 417
MaeII ACGT 4 cut(s) 126, 159, 270, 743
MaeIII GTNAC 5 cut(s) 379, 464, 674, 931, 1148
MalI GATC 3 cut(s) 828, 951, 1074
MbiI CCGCTC 2 cut(s) 179, 352
MboI GATC 3 cut(s) 826, 949, 1072
MboII GAAGA 3 cut(s) 346, 547, 774
MhlI GDGCHC 1 cut(s) 111
MlsI TGGCCA 2 cut(s) 5, 137
MluCI AATT 4 cut(s) 442, 706, 1097, 1121
MluNI TGGCCA 2 cut(s) 5, 137
MlyI GAGTC 2 cut(s) 65, 86
MmeI TCCRAC 2 cut(s) 36, 961
Mox20I TGGCCA 2 cut(s) 5, 137
MroI TCCGGA 1 cut(s) 718
MroNI GCCGGC 1 cut(s) 625
MroXI GAANNNNTTC 1 cut(s) 1097
MscI TGGCCA 2 cut(s) 5, 137
MseI TTAA 1 cut(s) 566
MslI CAYNNNNRTG 2 cut(s) 960, 1171
Msp20I TGGCCA 2 cut(s) 5, 137
MspA1I CMGCKG 1 cut(s) 296
MspR9I CCNGG 5 cut(s) 164, 193, 217, 585, 1103
MvaI CCWGG 2 cut(s) 193, 217
MvnI CGCG 4 cut(s) 144, 183, 243, 318
MwoI GCNNNNNNNGC 6 cut(s) 143, 173, 176, 317, 413, 1186
NaeI GCCGGC 1 cut(s) 627
NciI CCSGG 3 cut(s) 164, 585, 1103
NdeI CATATG 1 cut(s) 854
NdeII GATC 3 cut(s) 826, 949, 1072
NgoMIV GCCGGC 1 cut(s) 625
NlaIII CATG 7 cut(s) 136, 331, 343, 652, 950, 1051, 1156
NlaIV GGNNCC 2 cut(s) 214, 439
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 4 cut(s) 379, 464, 674, 1148
NruI TCGCGA 1 cut(s) 183
NspI RCATGY 1 cut(s) 1156
OliI CACNNNNGTG 1 cut(s) 1171
PciSI GCTCTTC 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdiI GCCGGC 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 1097
PfeI GAWTC 2 cut(s) 766, 779
PfoI TCCNGGA 1 cut(s) 1101
PleI GAGTC 2 cut(s) 65, 85
PpsI GAGTC 2 cut(s) 65, 85
PshAI GACNNNNGTC 2 cut(s) 386, 453
Psp6I CCWGG 2 cut(s) 191, 215
PspEI GGTNACC 1 cut(s) 674
PspFI CCCAGC 1 cut(s) 923
PspGI CCWGG 2 cut(s) 191, 215
PspN4I GGNNCC 2 cut(s) 214, 439
PspPI GGNCC 9 cut(s) 165, 212, 331, 347, 364, 437, 448, 460, 917
PsrI GAACNNNNNNTAC 2 cut(s) 564, 596
RruI TCGCGA 1 cut(s) 183
RseI CAYNNNNRTG 2 cut(s) 960, 1171
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 1 cut(s) 566
Sau3AI GATC 3 cut(s) 826, 949, 1072
Sau96I GGNCC 9 cut(s) 165, 212, 331, 347, 364, 437, 448, 460, 917
SchI GAGTC 2 cut(s) 65, 86
ScrFI CCNGG 5 cut(s) 164, 193, 217, 585, 1103
SduI GDGCHC 1 cut(s) 111
SfaNI GCATC 1 cut(s) 477
SfiI GGCCNNNNNGGCC 1 cut(s) 173
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 4 cut(s) 364, 448, 460, 917
SmiMI CAYNNNNRTG 2 cut(s) 960, 1171
SmlI CTYRAG 2 cut(s) 758, 1060
SmoI CTYRAG 2 cut(s) 758, 1060
Sse9I AATT 4 cut(s) 442, 706, 1097, 1121
SspMI CTAG 1 cut(s) 417
StyD4I CCNGG 5 cut(s) 162, 191, 215, 583, 1101
TaaI ACNGT 3 cut(s) 529, 685, 931
TaiI ACGT 4 cut(s) 129, 162, 273, 746
TaqI TCGA 2 cut(s) 69, 324
TaqII GACCGA 1 cut(s) 620
TasI AATT 4 cut(s) 442, 706, 1097, 1121
TfiI GAWTC 2 cut(s) 766, 779
Tru1I TTAA 1 cut(s) 566
Tru9I TTAA 1 cut(s) 566
TscAI CASTG 1 cut(s) 690
TseFI GTSAC 4 cut(s) 379, 464, 674, 1148
TseI GCWGC 2 cut(s) 199, 841
Tsp45I GTSAC 4 cut(s) 379, 464, 674, 1148
TspDTI ATGAA 2 cut(s) 774, 1002
TspGWI ACGGA 3 cut(s) 214, 452, 497
TspRI CASTG 1 cut(s) 690
VpaK11BI GGWCC 4 cut(s) 364, 448, 460, 917
XapI RAATTY 2 cut(s) 1097, 1121
XceI RCATGY 1 cut(s) 1156
XcmI CCANNNNNNNNNTGG 1 cut(s) 1173
XmnI GAANNNNTTC 1 cut(s) 1097
XspI CTAG 1 cut(s) 417
ZraI GACGTC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.