Rorug05G0459800

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005300
Physical Location & Seq
Reverse (-)
63307838 .. 63310113
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0459800.1

Sequence Viewer

Length: 822 bp
ATGACTAGCCAGTTATCTGATTTGACCAATATTCAAGATCTCATCTTGAAAAAGCTTGTGAGTTATCAGAAGGAAGACCTGGAAGATGTAGCTGCTCCCAAACTTCAGAACCTCACGGTTGAGTGCAATGACCTGAAAGGAGCTGAACCAAATGCACCGACCAGCCCAATCGCCAGCCCTCTCGACCCAGTTAAAAGAATTATAGATGGCTTCAAGCACTTCAAGACCAGCTACTTCGACACAAATCCAGCTTTGTTTGATGAGCTTGCCCAAGGACAAAGCCCCGAGTTTATGGTATTTGCATGCTCGGATTCTCGAGTGTGTCCTTCACATGTTCTTCACTTCCAACCTGGGGAGGCCTTCATCGTTCGCAACATTGCTAACATGGTTCCTGCTTTTGATCAGCTAAAACATGCAGGAGTTGGAGCAACCATAGAATATGCTGTCAAAGAACTCGGGGTGGCAAATATTTTGGTAATGGGACACAGTAATTGTGGTGGAATAAAGAGGCTAATGTCTTATCCGGAGGATGGCTCTGAGCCCTTCGACTTCATAGATGAATGGGTGAAAATGAGTTTACCAGCCAAGGCTGAGGTTATTGCAGAAGCTGGGAGTGCAGATTTCCATGAACAATGTGAAAGATGTGCAAGGGAATCAGTAAACTTGTCACTGGCAAACCTACTTACCTACCCCTTTGTTCAAAAGGCACACTTGGATAAAAAACTAGCACTTCGGGGTGGATACTATGACTTTGTCAATGGAATTTTCGAGCTATGGGAGCTCAAATCCCACATTTCAAACCCCATCATCGTACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000086 GO:0000278 GO:0003674 GO:0003682 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006807 GO:0006996 GO:0007049 GO:0007623 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008214 GO:0008276 GO:0008757 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016278 GO:0016279 GO:0016491 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016705 GO:0016706 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032259 GO:0032451 GO:0032452 GO:0034968 GO:0036211 GO:0042054 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0045893 GO:0045935 GO:0046975 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051213 GO:0051252 GO:0051254 GO:0051276 GO:0051864 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070544 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:0140110 GO:1901564 GO:1902680 GO:1903047 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_8

Protein Analysis

273

Amino Acids

30.23

Weight (kDa)

5.1

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 98 - 254 1e-41 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 523
AcsI RAATTY 1 cut(s) 762
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 1 cut(s) 813
AfiI CCNNNNNNNGG 2 cut(s) 352, 530
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 6 cut(s) 35, 49, 214, 223, 701, 798
AjnI CCWGG 2 cut(s) 78, 349
Alw21I GWGCWC 1 cut(s) 783
AlwNI CAGNNNCTG 1 cut(s) 608
Ama87I CYCGRG 3 cut(s) 284, 315, 455
Aor13HI TCCGGA 1 cut(s) 523
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 1 cut(s) 762
AsuHPI GGTGA 1 cut(s) 577
AvaI CYCGRG 3 cut(s) 284, 315, 455
BanII GRGCYC 2 cut(s) 543, 783
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 783
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 79
BccI CCATC 3 cut(s) 200, 524, 812
BciT130I CCWGG 2 cut(s) 80, 351
BciVI GTATCC 1 cut(s) 734
BclI TGATCA 1 cut(s) 400
BfaI CTAG 2 cut(s) 6, 725
BfuI GTATCC 1 cut(s) 734
BglII AGATCT 1 cut(s) 37
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme1390I CCNGG 2 cut(s) 80, 351
BmeT110I CYCGRG 3 cut(s) 284, 315, 455
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 2 cut(s) 80, 351
BmrI ACTGGG 1 cut(s) 182
BmuI ACTGGG 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 518, 550
Bpu10I CCTNAGC 1 cut(s) 591
BsaJI CCNNGG 3 cut(s) 271, 350, 585
BsaWI WCCGGW 1 cut(s) 523
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 530
Bse1I ACTGG 3 cut(s) 10, 188, 675
Bse3DI GCAATG 2 cut(s) 133, 375
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 2 cut(s) 80, 351
BseDI CCNNGG 3 cut(s) 271, 350, 585
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 2 cut(s) 352, 530
BseMI GCAATG 2 cut(s) 133, 375
BseMII CTCAG 2 cut(s) 528, 582
BseNI ACTGG 3 cut(s) 10, 188, 675
BseXI GCAGC 1 cut(s) 79
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 783
BsiHKCI CYCGRG 3 cut(s) 284, 315, 455
BsiSI CCGG 1 cut(s) 524
BslFI GGGAC 1 cut(s) 495
BslI CCNNNNNNNGG 2 cut(s) 352, 530
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 1 cut(s) 359
BsoBI CYCGRG 3 cut(s) 284, 315, 455
Bsp1286I GDGCHC 2 cut(s) 543, 783
Bsp13I TCCGGA 1 cut(s) 523
Bsp143I GATC 2 cut(s) 37, 400
BspANI GGCC 1 cut(s) 359
BspCNI CTCAG 2 cut(s) 529, 583
BspEI TCCGGA 1 cut(s) 523
BspLI GGNNCC 1 cut(s) 390
BsrDI GCAATG 2 cut(s) 133, 375
BsrI ACTGG 3 cut(s) 10, 188, 675
BssECI CCNNGG 3 cut(s) 271, 350, 585
BssMI GATC 2 cut(s) 37, 400
BssT1I CCWWGG 2 cut(s) 271, 585
Bst2UI CCWGG 2 cut(s) 80, 351
Bst4CI ACNGT 2 cut(s) 118, 488
BstC8I GCNNGC 3 cut(s) 175, 267, 304
BstDEI CTNAG 2 cut(s) 537, 591
BstF5I GGATG 1 cut(s) 535
BstKTI GATC 2 cut(s) 40, 403
BstMBI GATC 2 cut(s) 37, 400
BstMWI GCNNNNNNNGC 2 cut(s) 614, 778
BstNI CCWGG 2 cut(s) 80, 351
BstNSI RCATGY 3 cut(s) 306, 335, 416
BstSCI CCNGG 2 cut(s) 78, 349
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuI GTATCC 1 cut(s) 734
BsuRI GGCC 1 cut(s) 359
BtsCI GGATG 1 cut(s) 535
BtsIMutI CAGTG 1 cut(s) 668
Cac8I GCNNGC 3 cut(s) 175, 267, 304
CaiI CAGNNNCTG 1 cut(s) 608
Csp6I GTAC 1 cut(s) 812
CviAII CATG 5 cut(s) 303, 332, 385, 413, 626
CviQI GTAC 1 cut(s) 812
DdeI CTNAG 2 cut(s) 537, 591
DpnI GATC 2 cut(s) 39, 402
DpnII GATC 2 cut(s) 37, 400
Ecl136II GAGCTC 1 cut(s) 781
Eco130I CCWWGG 2 cut(s) 271, 585
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 2 cut(s) 543, 783
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 89
Eco88I CYCGRG 3 cut(s) 284, 315, 455
EcoICRI GAGCTC 1 cut(s) 781
EcoRII CCWGG 2 cut(s) 78, 349
EcoT14I CCWWGG 2 cut(s) 271, 585
EcoT38I GRGCYC 2 cut(s) 543, 783
ErhI CCWWGG 2 cut(s) 271, 585
FaeI CATG 5 cut(s) 306, 335, 388, 416, 629
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FaqI GGGAC 1 cut(s) 495
FatI CATG 5 cut(s) 302, 331, 384, 412, 625
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 1 cut(s) 93
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 2 cut(s) 543, 783
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 2 cut(s) 6, 725
GluI GCNGC 1 cut(s) 93
GsaI CCCAGC 1 cut(s) 612
HaeIII GGCC 1 cut(s) 359
HapII CCGG 1 cut(s) 524
Hin1II CATG 5 cut(s) 306, 335, 388, 416, 629
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 2 cut(s) 311, 653
HpaII CCGG 1 cut(s) 524
HphI GGTGA 1 cut(s) 577
Hpy166II GTNNAC 2 cut(s) 578, 661
Hpy188I TCNGA 5 cut(s) 19, 69, 108, 310, 538
Hpy188III TCNNGA 7 cut(s) 35, 46, 182, 223, 315, 524, 819
Hpy8I GTNNAC 2 cut(s) 578, 661
HpyAV CCTTC 4 cut(s) 64, 336, 370, 553
HpyCH4III ACNGT 2 cut(s) 118, 488
HpyCH4V TGCA 7 cut(s) 126, 155, 302, 416, 602, 617, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 614, 778
HpyF3I CTNAG 2 cut(s) 537, 591
Hsp92II CATG 5 cut(s) 306, 335, 388, 416, 629
Kpn2I TCCGGA 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 2 cut(s) 37, 400
LmnI GCTCC 4 cut(s) 100, 140, 425, 778
Lsp1109I GCAGC 1 cut(s) 79
MaeI CTAG 2 cut(s) 6, 725
MaeIII GTNAC 1 cut(s) 666
MalI GATC 2 cut(s) 39, 402
MboI GATC 2 cut(s) 37, 400
MboII GAAGA 3 cut(s) 86, 95, 329
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 543, 783
MluCI AATT 3 cut(s) 198, 490, 762
MmeI TCCRAC 2 cut(s) 370, 403
MnlI CCTC 6 cut(s) 122, 189, 349, 501, 520, 586
MroI TCCGGA 1 cut(s) 523
MseI TTAA 1 cut(s) 192
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 2 cut(s) 80, 351
MvaI CCWGG 2 cut(s) 80, 351
MwoI GCNNNNNNNGC 2 cut(s) 614, 778
NdeII GATC 2 cut(s) 37, 400
NlaIII CATG 5 cut(s) 306, 335, 388, 416, 629
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 666
NspI RCATGY 3 cut(s) 306, 335, 416
PaeI GCATGC 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 315
PceI AGGCCT 1 cut(s) 359
PciI ACATGT 1 cut(s) 331
PfeI GAWTC 2 cut(s) 311, 653
PflFI GACNNNGTC 1 cut(s) 752
PkrI GCNGC 1 cut(s) 94
PscI ACATGT 1 cut(s) 331
Psp124BI GAGCTC 1 cut(s) 783
Psp6I CCWGG 2 cut(s) 78, 349
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 2 cut(s) 78, 349
PspN4I GGNNCC 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 752
RsaI GTAC 1 cut(s) 813
RsaNI GTAC 1 cut(s) 812
SacI GAGCTC 1 cut(s) 783
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 2 cut(s) 37, 400
ScrFI CCNGG 2 cut(s) 80, 351
SduI GDGCHC 2 cut(s) 543, 783
Sfr274I CTCGAG 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
SphI GCATGC 1 cut(s) 306
Sse9I AATT 3 cut(s) 198, 490, 762
SseBI AGGCCT 1 cut(s) 359
SspI AATATT 2 cut(s) 31, 469
SspMI CTAG 2 cut(s) 6, 725
SstI GAGCTC 1 cut(s) 783
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 78, 349
StyI CCWWGG 2 cut(s) 271, 585
TaaI ACNGT 2 cut(s) 118, 488
TaqI TCGA 5 cut(s) 183, 237, 316, 546, 768
TasI AATT 3 cut(s) 198, 490, 762
TfiI GAWTC 2 cut(s) 311, 653
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 1 cut(s) 666
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 666
TspDTI ATGAA 4 cut(s) 352, 541, 573, 642
TspRI CASTG 1 cut(s) 675
Tth111I GACNNNGTC 1 cut(s) 752
XapI RAATTY 1 cut(s) 762
XceI RCATGY 3 cut(s) 306, 335, 416
XhoI CTCGAG 1 cut(s) 315
XspI CTAG 2 cut(s) 6, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.