RchiOBHm_Chr6g0263701

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009673
Physical Location & Seq
Reverse (-)
18574733 .. 18577649
2917 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23650

Sequence Viewer

Length: 1164 bp
ATGGAGAACATGAAGCAATTTGCTGGTCATACGAGAGTTGGTGATATTGAAGTATTAGTGGACAACATCTTGATGAACCTGCCTGTGAAGAACATATGCCAATGCCAACTGGTCTCTAAATCATGGCAAACAATGATAGCTGATTCTGAGTTCAATCACATGGTATGCCAACGTAATTGCCCGGTTAGCATATTTGTCAAATATGTTGATTTTCCCCAGACAGTATGCATCATTCATTACTCTAGTGATACTAGTTATTCTGTGGAAGAATTTGATCTCCCAAGTGGTTTCAAGGAAATTCTGGGCTCTTGCTATGGACTGATATGTCTAGGGGATGAAGAACAGCAGAAGCTATTGTTCTTGAATCCATTTACTAGGTGGATTCAAATCATACAAGAACTGGGAGATACCTCTGGAAACTATGGATTTGGATATGACCTGACTATTGAAGATTTCAAACTAGTCAAGCTGTTGGACAATTACTCTGCTCCTTATGACACAAGAAGAGTAATGATCTATTCAATGAAAGATCATTGTTGGAGAGAGAAATCATATGAAAGTTGGCAATTTAAATTGGACCCTTCAGCTGCCAATTTCGTCGCTGATAAACTATATTGGAGAGGGTTTTTATTGAAAACTCCACAACAACAAGTAGTAGAAGAAGATGTGCTGATCACTTTCAATATCTGCAGCGAGACTTTTGGAAGAGTTCGACTACCTGAAGAACTTTGTGGGCGGTATGTAACCTTTGGAGTGATCGAGAACAAGCTATCTGCTTGCCTCTCTGCTCAAGACATCTCCATATGGTTCTTGAATGACGATCAGTTTCAAAGAAGCATCACTTTGGCCAACTACAAAGCAAATCTTCATTGCTTAGTGCGGCCATTATATCTACGAGGCAATACCATCTTTCTTCTTCGAGCCAAAAGGAAGATTACCAAATACAACATCACTGAAGACTCTAAAGTTCAGATTTTGGGGTTTAACAAGACACCCGTTAGCCTTCAAGCTTTGGGTTTCGGTCTGATGTCGGAACCTAAAGCGAGAATGGAAGCCATTGGTTTTGTTCAAAGCTTATTTCCACTAGTATGCCAAAGGAATGGAAAACATGATGATGAGCCAAGTAAAGAAATTTCAAAAAGAAGAAAACTGTGTCCCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

387

Amino Acids

44.66

Weight (kDa)

6.61

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 19 - 52 6.8e-06 F-box domain
FBA_3 PF08268 87 - 275 1.5e-08 F-box associated beta propeller domain
FBA_1 PF07734 103 - 269 1.5e-12 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 324
Acc36I ACCTGC 1 cut(s) 87
AciI CCGC 2 cut(s) 736, 880
AcoI YGGCCR 2 cut(s) 846, 881
AcsI RAATTY 3 cut(s) 269, 297, 1131
AcuI CTGAAG 3 cut(s) 567, 741, 975
AhlI ACTAGT 3 cut(s) 251, 460, 1084
AluBI AGCT 7 cut(s) 140, 352, 469, 587, 769, 1010, 1074
AluI AGCT 7 cut(s) 140, 352, 469, 587, 769, 1010, 1074
Alw26I GTCTC 2 cut(s) 118, 689
AoxI GGCC 2 cut(s) 846, 881
ApeKI GCWGC 2 cut(s) 587, 690
ApoI RAATTY 3 cut(s) 269, 297, 1131
AspS9I GGNCC 1 cut(s) 577
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 53
AvaII GGWCC 1 cut(s) 577
BalI TGGCCA 1 cut(s) 848
BanII GRGCYC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 963
BbvI GCAGC 2 cut(s) 574, 702
BccI CCATC 1 cut(s) 914
BclI TGATCA 1 cut(s) 672
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 2 cut(s) 118, 689
BcuI ACTAGT 3 cut(s) 251, 460, 1084
BfaI CTAG 6 cut(s) 243, 252, 329, 375, 461, 1085
BfmI CTRYAG 1 cut(s) 688
BfuAI ACCTGC 1 cut(s) 87
BisI GCNGC 3 cut(s) 588, 691, 881
BlsI GCNGC 3 cut(s) 589, 692, 882
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 577
BmgT120I GGNCC 1 cut(s) 577
BmiI GGNNCC 2 cut(s) 579, 1035
BmrFI CCNGG 1 cut(s) 182
BmrI ACTGGG 1 cut(s) 410
BmsI GCATC 2 cut(s) 237, 846
BmuI ACTGGG 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 963
BpuEI CTTGAG 1 cut(s) 774
BpuMI CCSGG 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 118
Bse1I ACTGG 2 cut(s) 114, 405
Bse3DI GCAATG 1 cut(s) 868
Bse8I GATNNNNATC 1 cut(s) 386
BseGI GGATG 1 cut(s) 340
BseJI GATNNNNATC 1 cut(s) 386
BseMI GCAATG 1 cut(s) 868
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 2 cut(s) 114, 405
BseXI GCAGC 2 cut(s) 574, 702
BshFI GGCC 2 cut(s) 848, 883
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 1140
BsmAI GTCTC 2 cut(s) 118, 689
BsmFI GGGAC 1 cut(s) 1140
BsnI GGCC 2 cut(s) 848, 883
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 6 cut(s) 274, 513, 529, 672, 756, 820
BspACI CCGC 2 cut(s) 736, 880
BspANI GGCC 2 cut(s) 848, 883
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 579, 1035
BspMAI CTGCAG 1 cut(s) 692
BspMI ACCTGC 1 cut(s) 87
BspTNI GGTCTC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 868
BsrI ACTGG 2 cut(s) 114, 405
BssMI GATC 6 cut(s) 274, 513, 529, 672, 756, 820
Bst4CI ACNGT 2 cut(s) 223, 1152
Bst6I CTCTTC 2 cut(s) 499, 700
BstC8I GCNNGC 1 cut(s) 778
BstDEI CTNAG 2 cut(s) 147, 874
BstF5I GGATG 1 cut(s) 340
BstKTI GATC 6 cut(s) 277, 516, 532, 675, 759, 823
BstMAI GTCTC 2 cut(s) 118, 689
BstMBI GATC 6 cut(s) 274, 513, 529, 672, 756, 820
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 688
BstV1I GCAGC 2 cut(s) 574, 702
BstV2I GAAGAC 1 cut(s) 963
BstXI CCANNNNNNTGG 1 cut(s) 1100
BsuRI GGCC 2 cut(s) 848, 883
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 951
BveI ACCTGC 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 778
Cfr13I GGNCC 1 cut(s) 577
CviAII CATG 4 cut(s) 10, 123, 160, 1109
DdeI CTNAG 2 cut(s) 147, 874
DpnI GATC 6 cut(s) 276, 515, 531, 674, 758, 822
DpnII GATC 6 cut(s) 274, 513, 529, 672, 756, 820
DraI TTTAAA 1 cut(s) 571
DrdI GACNNNNNNGTC 1 cut(s) 324
DseDI GACNNNNNNGTC 1 cut(s) 324
EaeI YGGCCR 2 cut(s) 846, 881
Eam1104I CTCTTC 2 cut(s) 499, 700
EarI CTCTTC 2 cut(s) 499, 700
Eco24I GRGCYC 1 cut(s) 308
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 577
Eco57I CTGAAG 3 cut(s) 567, 741, 975
EcoT22I ATGCAT 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 308
FaeI CATG 4 cut(s) 13, 126, 163, 1112
FalI AAGNNNNNCTT 4 cut(s) 826, 858, 849, 881
FaqI GGGAC 1 cut(s) 1140
FatI CATG 4 cut(s) 9, 122, 159, 1108
FauNDI CATATG 3 cut(s) 95, 553, 803
FbaI TGATCA 1 cut(s) 672
Fnu4HI GCNGC 3 cut(s) 588, 691, 881
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 3 cut(s) 588, 691, 881
FspBI CTAG 6 cut(s) 243, 252, 329, 375, 461, 1085
GluI GCNGC 3 cut(s) 588, 691, 881
HaeIII GGCC 2 cut(s) 848, 883
HapII CCGG 1 cut(s) 182
Hin1II CATG 4 cut(s) 13, 126, 163, 1112
HindIII AAGCTT 2 cut(s) 1008, 1072
HinfI GANTC 4 cut(s) 143, 364, 382, 959
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 4 cut(s) 148, 972, 1026, 1033
Hpy188III TCNNGA 6 cut(s) 70, 361, 414, 760, 791, 811
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 2 cut(s) 591, 1013
HpyCH4III ACNGT 2 cut(s) 223, 1152
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 2 cut(s) 228, 690
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 2 cut(s) 147, 874
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 4 cut(s) 13, 126, 163, 1112
Ksp22I TGATCA 1 cut(s) 672
Kzo9I GATC 6 cut(s) 274, 513, 529, 672, 756, 820
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 2 cut(s) 574, 702
LweI GCATC 2 cut(s) 237, 846
MaeI CTAG 6 cut(s) 243, 252, 329, 375, 461, 1085
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 1 cut(s) 742
MalI GATC 6 cut(s) 276, 515, 531, 674, 758, 822
MboI GATC 6 cut(s) 274, 513, 529, 672, 756, 820
MhlI GDGCHC 1 cut(s) 308
MlsI TGGCCA 1 cut(s) 848
MluCI AATT 9 cut(s) 17, 175, 269, 297, 478, 566, 572, 592, 1131
MluNI TGGCCA 1 cut(s) 848
MlyI GAGTC 1 cut(s) 953
MmeI TCCRAC 3 cut(s) 453, 518, 1011
MnlI CCTC 4 cut(s) 421, 614, 791, 890
Mox20I TGGCCA 1 cut(s) 848
Mph1103I ATGCAT 1 cut(s) 230
MscI TGGCCA 1 cut(s) 848
MseI TTAA 2 cut(s) 570, 984
MslI CAYNNNNRTG 3 cut(s) 71, 1087, 1113
Msp20I TGGCCA 1 cut(s) 848
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 186
NciI CCSGG 1 cut(s) 182
NdeI CATATG 3 cut(s) 95, 553, 803
NdeII GATC 6 cut(s) 274, 513, 529, 672, 756, 820
NlaIII CATG 4 cut(s) 13, 126, 163, 1112
NlaIV GGNNCC 2 cut(s) 579, 1035
NsiI ATGCAT 1 cut(s) 230
PfeI GAWTC 3 cut(s) 143, 364, 382
PkrI GCNGC 3 cut(s) 589, 692, 882
PleI GAGTC 1 cut(s) 953
PpsI GAGTC 1 cut(s) 953
PspN4I GGNNCC 2 cut(s) 579, 1035
PspPI GGNCC 1 cut(s) 577
PstI CTGCAG 1 cut(s) 692
PvuII CAGCTG 1 cut(s) 587
RseI CAYNNNNRTG 3 cut(s) 71, 1087, 1113
SaqAI TTAA 2 cut(s) 570, 984
SatI GCNGC 3 cut(s) 588, 691, 881
Sau3AI GATC 6 cut(s) 274, 513, 529, 672, 756, 820
Sau96I GGNCC 1 cut(s) 577
SchI GAGTC 1 cut(s) 953
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 1 cut(s) 308
SfaNI GCATC 2 cut(s) 237, 846
SfcI CTRYAG 1 cut(s) 688
SinI GGWCC 1 cut(s) 577
SmiI ATTTAAAT 1 cut(s) 571
SmiMI CAYNNNNRTG 3 cut(s) 71, 1087, 1113
SmlI CTYRAG 1 cut(s) 789
SmoI CTYRAG 1 cut(s) 789
SpeI ACTAGT 3 cut(s) 251, 460, 1084
Sse9I AATT 9 cut(s) 17, 175, 269, 297, 478, 566, 572, 592, 1131
SsiI CCGC 2 cut(s) 736, 880
SspMI CTAG 6 cut(s) 243, 252, 329, 375, 461, 1085
StyD4I CCNGG 1 cut(s) 180
SwaI ATTTAAAT 1 cut(s) 571
TaaI ACNGT 2 cut(s) 223, 1152
TaiI ACGT 1 cut(s) 175
TaqI TCGA 3 cut(s) 712, 759, 919
TaqII GACCGA 1 cut(s) 1010
TasI AATT 9 cut(s) 17, 175, 269, 297, 478, 566, 572, 592, 1131
TauI GCSGC 1 cut(s) 883
TfiI GAWTC 3 cut(s) 143, 364, 382
Tru1I TTAA 2 cut(s) 570, 984
Tru9I TTAA 2 cut(s) 570, 984
TscAI CASTG 1 cut(s) 958
TseI GCWGC 2 cut(s) 587, 690
TspDTI ATGAA 7 cut(s) 26, 89, 224, 351, 539, 570, 857
TspRI CASTG 1 cut(s) 958
VpaK11BI GGWCC 1 cut(s) 577
XapI RAATTY 3 cut(s) 269, 297, 1131
XcmI CCANNNNNNNNNTGG 1 cut(s) 375
XspI CTAG 6 cut(s) 243, 252, 329, 375, 461, 1085
Zsp2I ATGCAT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.