RLG00000014321

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009673
Physical Location & Seq
Forward (+)
51298915 .. 51300346
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014321

Sequence Viewer

Length: 537 bp
ATGGAGAACATGAAGCAATTTGCTGGTCATAAGAGAGTTGGTGATATTGAAGTATTAGTGGACAACATCTTGATGAACCTGCCTGTGAAGAACATATGCCAATGCCAACTGGTCTCTAAATCATGGCAAACAATGATAGCTGATTCTGAGTTCAATCACATGGTATGCCAACGTAATTGCCCGGTTAGCGTATTTGTCAAATATGTTGATTTTCCCCAGACAGTATGCATCATTCATTACTCTAGTGATACTAGTTATTCTGTGGAAGAATTTGATCTCCCAAGTGGTTTCAAGGAAATTCTGGGCTCTTGCTATGGACTGATATGTCTAGGGGATGAAGAACAGCAGAAGCTATTGTTCTTGAATCCATTTACTAGGTGGATTCAAATCATACAAGAACTGGGAGATACCTCTGGAAACTATGGATTTGGATATGACCTGACTATTGAAGATTTCAAACTAGTCAAGCTGTTGGACAATTACTCTGCTCCTTATGACACAAGAAGACGAGACTTTTGGAAGAGTTCGACTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

179

Amino Acids

20.56

Weight (kDa)

4.9

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 19 - 52 2.1e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 324
Acc36I ACCTGC 1 cut(s) 87
AcsI RAATTY 2 cut(s) 269, 297
AgsI TTSAA 7 cut(s) 50, 154, 292, 364, 386, 449, 457
AhlI ACTAGT 2 cut(s) 251, 460
AluBI AGCT 3 cut(s) 140, 352, 469
AluI AGCT 3 cut(s) 140, 352, 469
Alw26I GTCTC 2 cut(s) 118, 504
ApoI RAATTY 2 cut(s) 269, 297
ArsI GACNNNNNNTTYG 2 cut(s) 498, 530
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 53
BanII GRGCYC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 511
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 2 cut(s) 118, 504
BcuI ACTAGT 2 cut(s) 251, 460
BfaI CTAG 5 cut(s) 243, 252, 329, 375, 461
BfuAI ACCTGC 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 182
BmrI ACTGGG 1 cut(s) 410
BmsI GCATC 1 cut(s) 237
BmuI ACTGGG 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 511
BpuMI CCSGG 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 118
Bse1I ACTGG 2 cut(s) 114, 405
Bse8I GATNNNNATC 1 cut(s) 386
BseGI GGATG 1 cut(s) 340
BseJI GATNNNNATC 1 cut(s) 386
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 2 cut(s) 114, 405
BsiSI CCGG 1 cut(s) 182
BsmAI GTCTC 2 cut(s) 118, 504
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 1 cut(s) 274
BspCNI CTCAG 1 cut(s) 139
BspMI ACCTGC 1 cut(s) 87
BspTNI GGTCTC 1 cut(s) 118
BsrI ACTGG 2 cut(s) 114, 405
BssMI GATC 1 cut(s) 274
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 515
BstDEI CTNAG 1 cut(s) 147
BstF5I GGATG 1 cut(s) 340
BstKTI GATC 1 cut(s) 277
BstMAI GTCTC 2 cut(s) 118, 504
BstMBI GATC 1 cut(s) 274
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 180
BstV2I GAAGAC 1 cut(s) 511
BtsCI GGATG 1 cut(s) 340
BveI ACCTGC 1 cut(s) 87
CviAII CATG 3 cut(s) 10, 123, 160
CviJI RGCY 4 cut(s) 140, 306, 352, 469
CviKI_1 RGCY 4 cut(s) 140, 306, 352, 469
DdeI CTNAG 1 cut(s) 147
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
DrdI GACNNNNNNGTC 1 cut(s) 324
DseDI GACNNNNNNGTC 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
Eco24I GRGCYC 1 cut(s) 308
Eco31I GGTCTC 1 cut(s) 118
EcoT22I ATGCAT 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 308
FaeI CATG 3 cut(s) 13, 126, 163
FatI CATG 3 cut(s) 9, 122, 159
FauNDI CATATG 1 cut(s) 95
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 308
FspBI CTAG 5 cut(s) 243, 252, 329, 375, 461
HapII CCGG 1 cut(s) 182
Hin1II CATG 3 cut(s) 13, 126, 163
HinfI GANTC 3 cut(s) 143, 364, 382
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 3 cut(s) 70, 361, 414
Hpy8I GTNNAC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 1 cut(s) 147
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 3 cut(s) 13, 126, 163
Kzo9I GATC 1 cut(s) 274
LmnI GCTCC 1 cut(s) 493
LweI GCATC 1 cut(s) 237
MaeI CTAG 5 cut(s) 243, 252, 329, 375, 461
MaeII ACGT 1 cut(s) 172
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MboII GAAGA 6 cut(s) 100, 278, 350, 461, 516, 532
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 5 cut(s) 17, 175, 269, 297, 478
MmeI TCCRAC 1 cut(s) 453
MnlI CCTC 1 cut(s) 421
Mph1103I ATGCAT 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 71
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 186
NciI CCSGG 1 cut(s) 182
NdeI CATATG 1 cut(s) 95
NdeII GATC 1 cut(s) 274
NlaIII CATG 3 cut(s) 13, 126, 163
NsiI ATGCAT 1 cut(s) 230
PfeI GAWTC 3 cut(s) 143, 364, 382
RseI CAYNNNNRTG 1 cut(s) 71
Sau3AI GATC 1 cut(s) 274
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 1 cut(s) 308
SetI ASST 9 cut(s) 81, 142, 175, 354, 380, 413, 441, 471, 536
SfaNI GCATC 1 cut(s) 237
SmiMI CAYNNNNRTG 1 cut(s) 71
SpeI ACTAGT 2 cut(s) 251, 460
Sse9I AATT 5 cut(s) 17, 175, 269, 297, 478
SspMI CTAG 5 cut(s) 243, 252, 329, 375, 461
StyD4I CCNGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 175
TaqI TCGA 1 cut(s) 527
TasI AATT 5 cut(s) 17, 175, 269, 297, 478
TfiI GAWTC 3 cut(s) 143, 364, 382
TspDTI ATGAA 4 cut(s) 26, 89, 224, 351
XapI RAATTY 2 cut(s) 269, 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 375
XspI CTAG 5 cut(s) 243, 252, 329, 375, 461
Zsp2I ATGCAT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.