Rh5CG521900

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009673
Physical Location & Seq
Reverse (-)
73208638 .. 73211627
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG521900.1

Sequence Viewer

Length: 1269 bp
ATGAAGAGGAAGCACTGTGAATGTTGCCATGACATATGCGATAGTGAAAGTTGTTGCACTGGCTGTAGCTCCAAGAGAAGACTGGAAAGAAAACTTGGAGACTGTAATGACCTGCCACCAAAGGCTGAACTTGGACATAGCAACGATTGTTTAGAGAGGATTCTTGTAGGGCAGCCACTGAGGATGTTAGCTCAATACCAGATGGTATCAAGACAGTGGAAGGCTCTCATTGGCCAACCAACATTTCAACAAATCCTTGCTGAAAAATCTGAGGCAAACAGCTTGTTTGTGAGTTATTTGAAGACTGATAAGGTAGGAATTGTAAGGCTGATTCACAACCGATATTCAGTGACCAATTATAGCCTCCCTCCATCTAGGTCTGCACTTCCCCTTAAGAATGTGTTAGGGTCATGCTACGGCTTGGTATGCTTGGGAAATACCAGCCGAGGAGGATACAGAGCTGAGGAGGATATACTCCTTCTCAACCCCTGCACAAAAAAATTGAGATTAATCAAGGATACAGTACATTGTGGACTGGAGAGGGGGCACTATGGCCTCGGTTTTGATAGAAAAGCACAAGATTTCAAAATTGCGAAAGTGTGCACTCCGCTGGTTAGGCACTCCAAGAAGAAGCTTACTCCAAAGAGGCAAGCAGAGATGGTGCATATTATAATCTACTCATGGAAAGATTCTTGCTGGCGTGATACTTATTTTGAAGATTGGGGTTTCAATTTGGTTGATTACCCGGGCATGTTTCTTGACGGCAAAATCTACTGGCCAGGGGAGGTTTGGGAGATGCAAGGGATATTTCTCAGACCACAGGGTCACCAAAACTCCTTGGTAAGCTTTGATGTGTCATCTGAAACGCTTGGCTGCATAAACCTGCCAGACGACCTCCAGTCTGAATATTGCAGCTTTGGAGTTGTTGCTGGAACATTGACTGCATGCGTCTGCGGAGAAAAAACCGAAGTTTGGCAATACACAGAGAATAATTTCAGAAAGAGGGCAAGTTTATCGACTTACAGAAATCATATGTCCTCTCACCTCCAGCCTCTATATCAGCTAGGGAATCTCATGGTTCTACTTCACAATGGCAGGAAAGTTACTCTATATGATATGTCTGACCACTCCAAGCTACATGCTTTGGGTCTTGACAAGTGGGTTCATCAAGGTGCTGACAATGGAAGAGGGCCAAGAATGGAAGCAGTGCCATTGGTTGAAAGCTTGTTTTCGCCAACCTTTGAGACAGAAAAGGCAAATTGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

422

Amino Acids

47.97

Weight (kDa)

8.49

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 116 - 301 2.8e-08 F-box associated beta propeller domain
FBA_1 PF07734 133 - 355 3.6e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
AasI GACNNNNNNGTC 1 cut(s) 822
Acc36I ACCTGC 2 cut(s) 120, 891
AciI CCGC 2 cut(s) 608, 954
AcoI YGGCCR 2 cut(s) 232, 776
AfaI GTAC 1 cut(s) 525
AfiI CCNNNNNNNGG 1 cut(s) 972
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 6 cut(s) 248, 301, 586, 716, 730, 1220
AhdI GACNNNNNGTC 1 cut(s) 898
AjnI CCWGG 1 cut(s) 778
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 228, 260
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 2 cut(s) 93, 1238
Alw44I GTGCAC 1 cut(s) 601
AlwNI CAGNNNCTG 1 cut(s) 178
Ama87I CYCGRG 1 cut(s) 745
AoxI GGCC 4 cut(s) 232, 553, 776, 1190
ApaLI GTGCAC 1 cut(s) 601
ApeKI GCWGC 3 cut(s) 172, 873, 912
AseI ATTAAT 1 cut(s) 509
Asp700I GAANNNNTTC 1 cut(s) 992
AspS9I GGNCC 1 cut(s) 1190
AsuC2I CCSGG 2 cut(s) 746, 747
AsuHPI GGTGA 2 cut(s) 818, 1034
AvaI CYCGRG 1 cut(s) 745
BaeGI GKGCMC 2 cut(s) 549, 605
BalI TGGCCA 2 cut(s) 234, 778
BbsI GAAGAC 2 cut(s) 85, 308
Bbv12I GWGCWC 1 cut(s) 605
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 3 cut(s) 184, 860, 924
BccI CCATC 3 cut(s) 196, 379, 652
BceAI ACGGC 2 cut(s) 433, 778
BcgI CGANNNNNNTGC 2 cut(s) 996, 1030
BciT130I CCWGG 1 cut(s) 780
BciVI GTATCC 2 cut(s) 446, 511
BcnI CCSGG 2 cut(s) 746, 747
BcoDI GTCTC 2 cut(s) 93, 1238
BfaI CTAG 2 cut(s) 375, 1064
BfmI CTRYAG 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 392
BfuAI ACCTGC 2 cut(s) 120, 891
BfuI GTATCC 2 cut(s) 446, 511
BisI GCNGC 3 cut(s) 173, 874, 913
BlsI GCNGC 3 cut(s) 174, 875, 914
Bme1390I CCNGG 3 cut(s) 746, 747, 780
BmeRI GACNNNNNGTC 1 cut(s) 898
BmeT110I CYCGRG 1 cut(s) 745
BmgT120I GGNCC 1 cut(s) 1190
BmrFI CCNGG 3 cut(s) 746, 747, 780
BmsI GCATC 1 cut(s) 786
BpiI GAAGAC 2 cut(s) 85, 308
BpmI CTGGAG 3 cut(s) 557, 881, 1031
Bpu10I CCTNAGC 1 cut(s) 462
BpuMI CCSGG 2 cut(s) 746, 747
BsaJI CCNNGG 5 cut(s) 445, 556, 745, 779, 837
BsaXI ACNNNNNCTCC 2 cut(s) 818, 848
Bsc4I CCNNNNNNNGG 1 cut(s) 972
Bse1I ACTGG 5 cut(s) 64, 87, 540, 779, 898
BseBI CCWGG 1 cut(s) 780
BseDI CCNNGG 5 cut(s) 445, 556, 745, 779, 837
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 972
BseMII CTCAG 4 cut(s) 170, 261, 453, 826
BseNI ACTGG 5 cut(s) 64, 87, 540, 779, 898
BseRI GAGGAG 2 cut(s) 462, 479
BseSI GKGCMC 2 cut(s) 549, 605
BseXI GCAGC 3 cut(s) 184, 860, 924
BsgI GTGCAG 2 cut(s) 366, 475
BshFI GGCC 4 cut(s) 234, 555, 778, 1192
BsiHKAI GWGCWC 1 cut(s) 605
BsiHKCI CYCGRG 1 cut(s) 745
BsiSI CCGG 1 cut(s) 746
BslI CCNNNNNNNGG 1 cut(s) 972
BsmAI GTCTC 2 cut(s) 93, 1238
BsnI GGCC 4 cut(s) 234, 555, 778, 1192
BsoBI CYCGRG 1 cut(s) 745
Bsp1286I GDGCHC 2 cut(s) 549, 605
BspACI CCGC 2 cut(s) 608, 954
BspANI GGCC 4 cut(s) 234, 555, 778, 1192
BspCNI CTCAG 4 cut(s) 171, 262, 454, 825
BspMI ACCTGC 2 cut(s) 120, 891
BspTI CTTAAG 1 cut(s) 392
BsrI ACTGG 5 cut(s) 64, 87, 540, 779, 898
BssECI CCNNGG 5 cut(s) 445, 556, 745, 779, 837
BssT1I CCWWGG 1 cut(s) 837
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 4 cut(s) 17, 104, 216, 523
Bst6I CTCTTC 1 cut(s) 1180
BstAFI CTTAAG 1 cut(s) 392
BstC8I GCNNGC 3 cut(s) 651, 698, 946
BstDEI CTNAG 4 cut(s) 179, 270, 462, 812
BstEII GGTNACC 1 cut(s) 824
BstF5I GGATG 1 cut(s) 189
BstMAI GTCTC 2 cut(s) 93, 1238
BstMWI GCNNNNNNNGC 2 cut(s) 426, 616
BstNI CCWGG 1 cut(s) 780
BstNSI RCATGY 3 cut(s) 754, 948, 1142
BstPI GGTNACC 1 cut(s) 824
BstSCI CCNGG 3 cut(s) 744, 745, 778
BstSFI CTRYAG 1 cut(s) 64
BstSLI GKGCMC 2 cut(s) 549, 605
BstV1I GCAGC 3 cut(s) 184, 860, 924
BstV2I GAAGAC 2 cut(s) 85, 308
BsuI GTATCC 2 cut(s) 446, 511
BsuRI GGCC 4 cut(s) 234, 555, 778, 1192
BtsCI GGATG 1 cut(s) 189
BtsI GCAGTG 1 cut(s) 1212
BtsIMutI CAGTG 6 cut(s) 13, 57, 176, 221, 354, 1212
BveI ACCTGC 2 cut(s) 120, 891
Cac8I GCNNGC 3 cut(s) 651, 698, 946
CaiI CAGNNNCTG 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 1190
Cfr9I CCCGGG 1 cut(s) 745
CseI GACGC 1 cut(s) 937
Csp6I GTAC 1 cut(s) 524
CviAII CATG 7 cut(s) 29, 411, 681, 751, 945, 1075, 1139
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 4 cut(s) 179, 270, 462, 812
DrdI GACNNNNNNGTC 1 cut(s) 822
DriI GACNNNNNGTC 1 cut(s) 898
DseDI GACNNNNNNGTC 1 cut(s) 822
EaeI YGGCCR 2 cut(s) 232, 776
Eam1104I CTCTTC 1 cut(s) 1180
Eam1105I GACNNNNNGTC 1 cut(s) 898
EarI CTCTTC 1 cut(s) 1180
Eco130I CCWWGG 1 cut(s) 837
Eco88I CYCGRG 1 cut(s) 745
Eco91I GGTNACC 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 824
EcoRII CCWGG 1 cut(s) 778
EcoT14I CCWWGG 1 cut(s) 837
ErhI CCWWGG 1 cut(s) 837
FaeI CATG 7 cut(s) 32, 414, 684, 754, 948, 1078, 1142
FatI CATG 7 cut(s) 28, 410, 680, 750, 944, 1074, 1138
FauNDI CATATG 2 cut(s) 35, 1032
Fnu4HI GCNGC 3 cut(s) 173, 874, 913
FokI GGATG 1 cut(s) 196
Fsp4HI GCNGC 3 cut(s) 173, 874, 913
FspBI CTAG 2 cut(s) 375, 1064
GluI GCNGC 3 cut(s) 173, 874, 913
GsuI CTGGAG 3 cut(s) 557, 881, 1031
HaeIII GGCC 4 cut(s) 234, 555, 778, 1192
HapII CCGG 1 cut(s) 746
HgaI GACGC 1 cut(s) 937
Hin1II CATG 7 cut(s) 32, 414, 684, 754, 948, 1078, 1142
HindIII AAGCTT 3 cut(s) 632, 844, 1222
HinfI GANTC 4 cut(s) 160, 331, 689, 1069
HpaII CCGG 1 cut(s) 746
HphI GGTGA 2 cut(s) 818, 1034
Hpy166II GTNNAC 2 cut(s) 533, 603
Hpy188I TCNGA 6 cut(s) 271, 815, 862, 904, 998, 1123
Hpy188III TCNNGA 3 cut(s) 210, 758, 1151
Hpy8I GTNNAC 2 cut(s) 533, 603
HpyAV CCTTC 2 cut(s) 214, 488
HpyCH4III ACNGT 4 cut(s) 17, 104, 216, 523
HpyCH4V TGCA 9 cut(s) 57, 383, 492, 603, 664, 799, 876, 912, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 426, 616
HpyF3I CTNAG 4 cut(s) 179, 270, 462, 812
Hsp92II CATG 7 cut(s) 32, 414, 684, 754, 948, 1078, 1142
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 3 cut(s) 184, 860, 924
LweI GCATC 1 cut(s) 786
MaeI CTAG 2 cut(s) 375, 1064
MaeIII GTNAC 3 cut(s) 349, 824, 1102
MboII GAAGA 6 cut(s) 16, 90, 313, 640, 728, 1197
MhlI GDGCHC 2 cut(s) 549, 605
MlsI TGGCCA 2 cut(s) 234, 778
MluCI AATT 7 cut(s) 318, 355, 500, 588, 730, 991, 1258
MluNI TGGCCA 2 cut(s) 234, 778
Mox20I TGGCCA 2 cut(s) 234, 778
MroXI GAANNNNTTC 1 cut(s) 992
MscI TGGCCA 2 cut(s) 234, 778
MseI TTAA 2 cut(s) 393, 509
MslI CAYNNNNRTG 1 cut(s) 1170
Msp20I TGGCCA 2 cut(s) 234, 778
MspA1I CMGCKG 1 cut(s) 610
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 1 cut(s) 746
MspR9I CCNGG 3 cut(s) 746, 747, 780
MvaI CCWGG 1 cut(s) 780
MwoI GCNNNNNNNGC 2 cut(s) 426, 616
NciI CCSGG 2 cut(s) 746, 747
NdeI CATATG 2 cut(s) 35, 1032
NlaIII CATG 7 cut(s) 32, 414, 684, 754, 948, 1078, 1142
NmeAIII GCCGAG 1 cut(s) 470
NmuCI GTSAC 2 cut(s) 349, 824
NspI RCATGY 3 cut(s) 754, 948, 1142
PaeI GCATGC 1 cut(s) 948
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 4 cut(s) 160, 331, 689, 1069
PkrI GCNGC 3 cut(s) 174, 875, 914
PshBI ATTAAT 1 cut(s) 509
PsiI TTATAA 1 cut(s) 671
Psp6I CCWGG 1 cut(s) 778
PspEI GGTNACC 1 cut(s) 824
PspGI CCWGG 1 cut(s) 778
PspPI GGNCC 1 cut(s) 1190
PstNI CAGNNNCTG 1 cut(s) 178
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
RseI CAYNNNNRTG 1 cut(s) 1170
SaqAI TTAA 2 cut(s) 393, 509
SatI GCNGC 3 cut(s) 173, 874, 913
Sau96I GGNCC 1 cut(s) 1190
ScrFI CCNGG 3 cut(s) 746, 747, 780
SduI GDGCHC 2 cut(s) 549, 605
SfaNI GCATC 1 cut(s) 786
SfcI CTRYAG 1 cut(s) 64
SmaI CCCGGG 1 cut(s) 747
SmiMI CAYNNNNRTG 1 cut(s) 1170
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SphI GCATGC 1 cut(s) 948
Sse9I AATT 7 cut(s) 318, 355, 500, 588, 730, 991, 1258
SsiI CCGC 2 cut(s) 608, 954
SspI AATATT 1 cut(s) 908
SspMI CTAG 2 cut(s) 375, 1064
StyD4I CCNGG 3 cut(s) 744, 745, 778
StyI CCWWGG 1 cut(s) 837
TaaI ACNGT 4 cut(s) 17, 104, 216, 523
TaqI TCGA 1 cut(s) 1016
TasI AATT 7 cut(s) 318, 355, 500, 588, 730, 991, 1258
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 4 cut(s) 160, 331, 689, 1069
Tru1I TTAA 2 cut(s) 393, 509
Tru9I TTAA 2 cut(s) 393, 509
TscAI CASTG 6 cut(s) 20, 64, 183, 221, 354, 1212
TseFI GTSAC 2 cut(s) 349, 824
TseI GCWGC 3 cut(s) 172, 873, 912
Tsp45I GTSAC 2 cut(s) 349, 824
TspDTI ATGAA 2 cut(s) 17, 1154
TspMI CCCGGG 1 cut(s) 745
TspRI CASTG 6 cut(s) 20, 64, 183, 221, 354, 1212
Vha464I CTTAAG 1 cut(s) 392
VneI GTGCAC 1 cut(s) 601
VspI ATTAAT 1 cut(s) 509
XceI RCATGY 3 cut(s) 754, 948, 1142
XcmI CCANNNNNNNNNTGG 2 cut(s) 79, 786
XmaI CCCGGG 1 cut(s) 745
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 2 cut(s) 375, 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.