RLG00000014325

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009673
Physical Location & Seq
Forward (+)
51371050 .. 51372401
1352 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014325

Sequence Viewer

Length: 528 bp
ATGAAGCAATTTGCTGGTCATAAGAGAGTTGGTGATATTGAAGTATTAGTGGACAACATCTTGATGAACCTGCCTGTGAAGAACATATGCCAATGCCAACTGGTCTCTAAATCATGGCAAACAATGATAGCTGATTCTGAGTTCAATCACATGGTATGCCAACGTAATTGCCCGGTTAGCGTATTTGTCAAATATGTTGATTTTCCCCAGACAGTATGCATCATTCATTACTCTAGTGATACTAGTTATTCTGTGGAAGAATTTGATCTCCCAAGTGGTTTCAAGGAAATTCTGGGCTCTTGCTATGGACTGATATGTCTAGGGGATGAAGAACAGCAGAAGCTATTGTTCTTGAATCCATTTACTAGGTGGATTCAAATCATACAAGAACTGGGAGATACCTCTGGAAACTATGGATTTGGATATGACCTGACTATTGAAGATTTCAAACTAGTCAAGCTGTTGGACAATTACTCTGCTCCTTATGACACAAGAAGACGAGACTTTTGGAAGAGTTCGACTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

176

Amino Acids

20.18

Weight (kDa)

5.0

Isoelectric Point (pI)

56.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 16 - 49 2.1e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 315
Acc36I ACCTGC 1 cut(s) 78
AcsI RAATTY 2 cut(s) 260, 288
AgsI TTSAA 7 cut(s) 41, 145, 283, 355, 377, 440, 448
AhlI ACTAGT 2 cut(s) 242, 451
AluBI AGCT 3 cut(s) 131, 343, 460
AluI AGCT 3 cut(s) 131, 343, 460
Alw26I GTCTC 2 cut(s) 109, 495
ApoI RAATTY 2 cut(s) 260, 288
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 44
BanII GRGCYC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 502
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 2 cut(s) 109, 495
BcuI ACTAGT 2 cut(s) 242, 451
BfaI CTAG 5 cut(s) 234, 243, 320, 366, 452
BfuAI ACCTGC 1 cut(s) 78
Bme1390I CCNGG 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 502
BpuMI CCSGG 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 109
Bse1I ACTGG 2 cut(s) 105, 396
Bse8I GATNNNNATC 1 cut(s) 377
BseGI GGATG 1 cut(s) 331
BseJI GATNNNNATC 1 cut(s) 377
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 2 cut(s) 105, 396
BsiSI CCGG 1 cut(s) 173
BsmAI GTCTC 2 cut(s) 109, 495
Bso31I GGTCTC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 1 cut(s) 265
BspCNI CTCAG 1 cut(s) 130
BspMI ACCTGC 1 cut(s) 78
BspTNI GGTCTC 1 cut(s) 109
BsrI ACTGG 2 cut(s) 105, 396
BssMI GATC 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 506
BstDEI CTNAG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 2 cut(s) 109, 495
BstMBI GATC 1 cut(s) 265
BstMWI GCNNNNNNNGC 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 171
BstV2I GAAGAC 1 cut(s) 502
BtsCI GGATG 1 cut(s) 331
BveI ACCTGC 1 cut(s) 78
CviAII CATG 2 cut(s) 114, 151
CviJI RGCY 4 cut(s) 131, 297, 343, 460
CviKI_1 RGCY 4 cut(s) 131, 297, 343, 460
DdeI CTNAG 1 cut(s) 138
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
DrdI GACNNNNNNGTC 1 cut(s) 315
DseDI GACNNNNNNGTC 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
Eco24I GRGCYC 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 299
FaeI CATG 2 cut(s) 117, 154
FatI CATG 2 cut(s) 113, 150
FauNDI CATATG 1 cut(s) 86
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 1 cut(s) 299
FspBI CTAG 5 cut(s) 234, 243, 320, 366, 452
HapII CCGG 1 cut(s) 173
Hin1II CATG 2 cut(s) 117, 154
HinfI GANTC 3 cut(s) 134, 355, 373
HpaII CCGG 1 cut(s) 173
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 3 cut(s) 61, 352, 405
Hpy8I GTNNAC 1 cut(s) 52
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 1 cut(s) 177
HpyF3I CTNAG 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 2 cut(s) 117, 154
Kzo9I GATC 1 cut(s) 265
LmnI GCTCC 1 cut(s) 484
LpnPI CCDG 9 cut(s) 83, 86, 87, 186, 221, 278, 377, 390, 443
LweI GCATC 1 cut(s) 228
MaeI CTAG 5 cut(s) 234, 243, 320, 366, 452
MaeII ACGT 1 cut(s) 163
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 6 cut(s) 91, 269, 341, 452, 507, 523
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 5 cut(s) 8, 166, 260, 288, 469
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 1 cut(s) 412
Mph1103I ATGCAT 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 62
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 173
MwoI GCNNNNNNNGC 1 cut(s) 177
NciI CCSGG 1 cut(s) 173
NdeI CATATG 1 cut(s) 86
NdeII GATC 1 cut(s) 265
NlaIII CATG 2 cut(s) 117, 154
NsiI ATGCAT 1 cut(s) 221
PfeI GAWTC 3 cut(s) 134, 355, 373
RseI CAYNNNNRTG 1 cut(s) 62
Sau3AI GATC 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 1 cut(s) 299
SetI ASST 9 cut(s) 72, 133, 166, 345, 371, 404, 432, 462, 527
SfaNI GCATC 1 cut(s) 228
SmiMI CAYNNNNRTG 1 cut(s) 62
SpeI ACTAGT 2 cut(s) 242, 451
Sse9I AATT 5 cut(s) 8, 166, 260, 288, 469
SspMI CTAG 5 cut(s) 234, 243, 320, 366, 452
StyD4I CCNGG 1 cut(s) 171
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 166
TaqI TCGA 1 cut(s) 518
TasI AATT 5 cut(s) 8, 166, 260, 288, 469
TfiI GAWTC 3 cut(s) 134, 355, 373
TspDTI ATGAA 4 cut(s) 17, 80, 215, 342
XapI RAATTY 2 cut(s) 260, 288
XcmI CCANNNNNNNNNTGG 1 cut(s) 366
XspI CTAG 5 cut(s) 234, 243, 320, 366, 452
Zsp2I ATGCAT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.