Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0003147

Physical Location & Seq

Genomic Coordinates Forward (+)

58180621 .. 58188343

Gene Structure 7723 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014834

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGATTCCCTCTCAGTTGGTGTGGCATATTGCCATTGGGTGTTACTGGAGTTGACACCAAGTTCTGGATCCTGTGATCCTTTATGTTCGGTTGACGAAATGAGCTCAATTGATTTTGAGGCTTGTAATTTTGAGGCCAGTTACAAGCCAAAGACTTACTTGCTGAAAGCTATAGCAATATTTGCTGCAGCTGCAACAGGGACGGTGGCAATTAACCACTCATGGGTTGCTGCTAATCAGGATGTTGCCATGGCACTGCTATTTGGAATAGGATATGCAGGCATTATTTTTGAAGAGTCTCTGGCCTTCAACAAGAGTGGAGTAGGTTTGATAATGGCTGTATGCTTGTGGATAGTGAGAAGTATTAGTGCTCCTTCAACTGATATAGCTGTTGCAGAGTTACAATATGCATCTGCTGAAGTCAGTGAAATAGTGTTTTTCTTGCTTGGTGCAATGACAATTGTAGAGATAGTTGATTCTCATCAAGGATTTGAGCTGATCACTGACAATATAACTACTCGAAAGCCAAGAACACTCCTCTGGGTGGTTGGTTTTGTGACTTTCTTCCTTGGTTCAATTCTTGACAACCTGACGTGTACCATTGTCATGGTCTCTTTATTGCGAAAACTAGTGCCTCCCTCAGACTTCCGCAAGATTTTAGGAGGAGTTGTTGTCATAGCTGCAAATGCTGGTGGTACGTGGACTCCTATTGGTGATGTTACTACCACTATGTTATGGATACACGGTCAGATATCAACAGTGGAGACAATGAAGGTATTATTTATACCAGCAGTCGTTTCTGTTGCTGTTCCACTGGCACTTTTGTCCCTTACAAGTGAAGCAAGTGGTGATGGACCAGACACATCCAATGTTATGGCATCCAAACAGATGGCTCCCCAGGGACAGCTAATTGTTTTCTTTGTAGGCTTCGGAGCTTTGATTTTTGTTCCAGTTTTCAAGTCCCTAACTGGTTTGCCTCCTTACATGGGTATGCTGATGGGACTTGGAGTCCTTTGGATGCTGACTGATGCAATCCACTGTGGTGAATCGCAAAGACAAAGGTTGAAAGTACCTCAGGCTTTGTCACGGATTGACACTCAAGGAGCGCTTTTCTTCCTTGGAATTCTTTTATCTGTTAGCAGCTTGGAGGCGGCAGGAATCCTTCAGGAATTAGCAAACTACCTGGATGCTCATATTCCCAATATTGAACTGACTGCAATTTCAATAGGAATTGTCTCAGCAATTATAGACAATGTTCCACTGGTTGCTGCAGCCATGGGGATGTACGATGTTGCCTCTTTTCCCCAGGACTCTGAGTTTTGGCAGCTGGTTGCATATTGTGCTGGTACTGGTGGATCCATGCTAGTTATTGGTTCTGCAGCCGGAGTTGCTTTTATGGGGATGGAAAAGGTGGACTTCTTCTGGTACTTTCGCAAGGTGAGTGGATTTGCTTTTGCGGGTTATGCTGCTGGTATTGCAGCCTATTTAGCAGCTCAAAACCTCCACATCTCAATACCAACAACCGTAGCTCAGGGTCCTTTTCTTTCTGGATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006814 GO:0006820 GO:0006848 GO:0006950 GO:0006970 GO:0008028 GO:0008150 GO:0008509 GO:0008514 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009651 GO:0009941 GO:0015075 GO:0015291 GO:0015297 GO:0015318 GO:0015672 GO:0015711 GO:0015718 GO:0015849 GO:0022804 GO:0022857 GO:0030001 GO:0031967 GO:0031975 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0050833 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098656 GO:1901475 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CitMHS

Protein Analysis

519

Amino Acids

55.0

Weight (kDa)

4.71

Isoelectric Point (pI)

34.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CitMHS	PF03600	93 - 434	3.4e-25	Citrate transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0003147)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49810 AT3G19490
fragaria_vesca	FvH4_2g05320 FvH4_2g05320 FvH4_2g05320 FvH4_2g05320 FvH4_2g05320 FvH4_2g05320 FvH4_2g05320
malus_domestica	MD05G1088200.v1.1
prunus_persica	Prupe.8G134200_v2.0.a1 Prupe.8G134200_v2.0.a1 Prupe.8G134200_v2.0.a1 Prupe.8G134200_v2.0.a1
pyrus_communis	pycom05g08760 pycom10g08240
rosa_chinensis	RchiOBHm_Chr6g0255461 RchiOBHm_Chr6g0255491 RchiOBHm_Chr6g0255511 RchiOBHm_Chr6g0255521
rosa_laevigata	RLG00000014834 RLG00000014835
rosa_multiflora	Rmu_sc0002360.1_g000008
rosa_roxburghii	Rroxscaffold_7G00209940 Rroxscaffold_7G00209960
rosa_rugosa	Rorug05G0559000 Rorug05G0559100 Rorug05G0559100
rosa_samantha	Rh6AG076700 Rh6AG077100 Rh6AG077200 Rh6BG069500 Rh6BG069800 Rh6CG068500 Rh6DG065300
rosa_wichuraiana	Rw6G006660 Rw6G006680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	65
AciI	CCGC	3 cut(s)	649, 1151, 1457
AclWI	GGATC	6 cut(s)	63, 71, 76, 1350, 1363, 1545
AcsI	RAATTY	1 cut(s)	1122
AcuI	CTGAAG	2 cut(s)	438, 1148
AfaI	GTAC	6 cut(s)	598, 697, 1071, 1286, 1348, 1427
AfeI	AGCGCT	1 cut(s)	1107
AfiI	CCNNNNNNNGG	4 cut(s)	65, 223, 544, 986
AflIII	ACRYGT	1 cut(s)	593
AgsI	TTSAA	8 cut(s)	293, 310, 378, 576, 958, 1066, 1208, 1224
AhdI	GACNNNNNGTC	1 cut(s)	1007
AhlI	ACTAGT	1 cut(s)	628
AjiI	CACGTC	1 cut(s)	594
AjnI	CCWGG	3 cut(s)	897, 1182, 1305
AleI	CACNNNNGTG	1 cut(s)	1041
Alw21I	GWGCWC	2 cut(s)	107, 373
Alw26I	GTCTC	4 cut(s)	303, 616, 758, 1240
AlwI	GGATC	6 cut(s)	63, 71, 76, 1350, 1363, 1545
Aor51HI	AGCGCT	1 cut(s)	1107
AoxI	GGCC	2 cut(s)	135, 303
ApoI	RAATTY	1 cut(s)	1122
AspLEI	GCGC	1 cut(s)	1108
AspS9I	GGNCC	2 cut(s)	854, 1535
AsuHPI	GGTGA	4 cut(s)	725, 860, 1055, 1450
AvaII	GGWCC	2 cut(s)	854, 1535
AxyI	CCTNAGG	1 cut(s)	1074
BamHI	GGATCC	3 cut(s)	68, 1355, 1550
BanII	GRGCYC	1 cut(s)	107
Bbv12I	GWGCWC	2 cut(s)	107, 373
BccI	CCATC	4 cut(s)	845, 883, 991, 1396
BciT130I	CCWGG	3 cut(s)	899, 1184, 1307
BciVI	GTATCC	1 cut(s)	732
BclI	TGATCA	1 cut(s)	498
BcoDI	GTCTC	4 cut(s)	303, 616, 758, 1240
BcuI	ACTAGT	1 cut(s)	628
BfaI	CTAG	2 cut(s)	629, 1364
BfmI	CTRYAG	4 cut(s)	171, 186, 1269, 1377
BfoI	RGCGCY	1 cut(s)	1109
BfuI	GTATCC	1 cut(s)	732
Bme1390I	CCNGG	3 cut(s)	899, 1184, 1307
Bme18I	GGWCC	2 cut(s)	854, 1535
BmeRI	GACNNNNNGTC	1 cut(s)	1007
BmgBI	CACGTC	1 cut(s)	594
BmgT120I	GGNCC	2 cut(s)	854, 1535
BmiI	GGNNCC	5 cut(s)	70, 894, 1357, 1536, 1552
BmrFI	CCNGG	3 cut(s)	899, 1184, 1307
BmsI	GCATC	5 cut(s)	419, 887, 1008, 1018, 1177
BpmI	CTGGAG	1 cut(s)	68
Bpu10I	CCTNAGC	1 cut(s)	1530
BpuEI	CTTGAG	1 cut(s)	1083
BsaAI	YACGTR	1 cut(s)	699
BsaBI	GATNNNNATC	1 cut(s)	480
BsaI	GGTCTC	1 cut(s)	616
BsaJI	CCNNGG	7 cut(s)	249, 568, 897, 898, 1117, 1275, 1305
BsaXI	ACNNNNNCTCC	6 cut(s)	654, 657, 684, 687, 688, 718
Bsc4I	CCNNNNNNNGG	4 cut(s)	65, 223, 544, 986
Bse1I	ACTGG	7 cut(s)	51, 138, 819, 950, 973, 1266, 1354
Bse21I	CCTNAGG	1 cut(s)	1074
Bse3DI	GCAATG	1 cut(s)	459
Bse8I	GATNNNNATC	1 cut(s)	480
BseBI	CCWGG	3 cut(s)	899, 1184, 1307
BseDI	CCNNGG	7 cut(s)	249, 568, 897, 898, 1117, 1275, 1305
BseGI	GGATG	7 cut(s)	247, 863, 878, 1023, 1192, 1287, 1407
BseJI	GATNNNNATC	1 cut(s)	480
BseLI	CCNNNNNNNGG	4 cut(s)	65, 223, 544, 986
BseMI	GCAATG	1 cut(s)	459
BseMII	CTCAG	6 cut(s)	27, 654, 1088, 1251, 1305, 1544
BseNI	ACTGG	7 cut(s)	51, 138, 819, 950, 973, 1266, 1354
BseRI	GAGGAG	2 cut(s)	527, 678
BshFI	GGCC	2 cut(s)	137, 305
BsiHKAI	GWGCWC	2 cut(s)	107, 373
BsiSI	CCGG	1 cut(s)	1383
BslFI	GGGAC	5 cut(s)	214, 811, 915, 946, 1014
BslI	CCNNNNNNNGG	4 cut(s)	65, 223, 544, 986
BsmAI	GTCTC	4 cut(s)	303, 616, 758, 1240
BsmFI	GGGAC	5 cut(s)	214, 811, 915, 946, 1014
BsnI	GGCC	2 cut(s)	137, 305
Bso31I	GGTCTC	1 cut(s)	616
Bsp1286I	GDGCHC	2 cut(s)	107, 373
Bsp143I	GATC	5 cut(s)	68, 76, 498, 1355, 1550
Bsp19I	CCATGG	2 cut(s)	249, 1275
BspACI	CCGC	3 cut(s)	649, 1151, 1457
BspANI	GGCC	2 cut(s)	137, 305
BspCNI	CTCAG	6 cut(s)	26, 653, 1087, 1250, 1306, 1543
BspLI	GGNNCC	5 cut(s)	70, 894, 1357, 1536, 1552
BspMAI	CTGCAG	3 cut(s)	190, 1273, 1381
BspPI	GGATC	6 cut(s)	63, 71, 76, 1350, 1363, 1545
BspTNI	GGTCTC	1 cut(s)	616
BsrDI	GCAATG	1 cut(s)	459
BsrI	ACTGG	7 cut(s)	51, 138, 819, 950, 973, 1266, 1354
BssECI	CCNNGG	7 cut(s)	249, 568, 897, 898, 1117, 1275, 1305
BssMI	GATC	5 cut(s)	68, 76, 498, 1355, 1550
BssT1I	CCWWGG	4 cut(s)	249, 568, 1117, 1275
Bst2UI	CCWGG	3 cut(s)	899, 1184, 1307
Bst4CI	ACNGT	5 cut(s)	205, 746, 760, 1040, 1525
Bst6I	CTCTTC	1 cut(s)	288
BstAPI	GCANNNNNTGC	2 cut(s)	182, 1340
BstBAI	YACGTR	1 cut(s)	699
BstC8I	GCNNGC	1 cut(s)	280
BstDEI	CTNAG	6 cut(s)	13, 640, 1074, 1237, 1314, 1530
BstDSI	CCRYGG	2 cut(s)	249, 1275
BstF5I	GGATG	7 cut(s)	247, 863, 878, 1023, 1192, 1287, 1407
BstH2I	RGCGCY	1 cut(s)	1109
BstHHI	GCGC	1 cut(s)	1108
BstKTI	GATC	5 cut(s)	71, 79, 501, 1358, 1553
BstMAI	GTCTC	4 cut(s)	303, 616, 758, 1240
BstMBI	GATC	5 cut(s)	68, 76, 498, 1355, 1550
BstMWI	GCNNNNNNNGC	8 cut(s)	182, 191, 686, 1340, 1388, 1463, 1475, 1487
BstNI	CCWGG	3 cut(s)	899, 1184, 1307
BstSCI	CCNGG	3 cut(s)	897, 1182, 1305
BstSFI	CTRYAG	4 cut(s)	171, 186, 1269, 1377
BstX2I	RGATCY	3 cut(s)	68, 1355, 1550
BstXI	CCANNNNNNTGG	3 cut(s)	607, 874, 889
BstYI	RGATCY	3 cut(s)	68, 1355, 1550
Bsu36I	CCTNAGG	1 cut(s)	1074
BsuI	GTATCC	1 cut(s)	732
BsuRI	GGCC	2 cut(s)	137, 305
BtgI	CCRYGG	2 cut(s)	249, 1275
BtrI	CACGTC	1 cut(s)	594
BtsCI	GGATG	7 cut(s)	247, 863, 878, 1023, 1192, 1287, 1407
BtsI	GCAGTG	1 cut(s)	254
BtsIMutI	CAGTG	7 cut(s)	254, 430, 501, 765, 812, 1036, 1259
Cac8I	GCNNGC	1 cut(s)	280
CfoI	GCGC	1 cut(s)	1108
Cfr13I	GGNCC	2 cut(s)	854, 1535
Csp6I	GTAC	6 cut(s)	597, 696, 1070, 1285, 1347, 1426
CspCI	CAANNNNNGTGG	4 cut(s)	298, 333, 1423, 1458
CviAII	CATG	6 cut(s)	222, 250, 607, 985, 1276, 1360
CviQI	GTAC	6 cut(s)	597, 696, 1070, 1285, 1347, 1426
DdeI	CTNAG	6 cut(s)	13, 640, 1074, 1237, 1314, 1530
DpnI	GATC	5 cut(s)	70, 78, 500, 1357, 1552
DpnII	GATC	5 cut(s)	68, 76, 498, 1355, 1550
DriI	GACNNNNNGTC	1 cut(s)	1007
Eam1104I	CTCTTC	1 cut(s)	288
Eam1105I	GACNNNNNGTC	1 cut(s)	1007
EarI	CTCTTC	1 cut(s)	288
Ecl136II	GAGCTC	1 cut(s)	105
Eco130I	CCWWGG	4 cut(s)	249, 568, 1117, 1275
Eco24I	GRGCYC	1 cut(s)	107
Eco31I	GGTCTC	1 cut(s)	616
Eco32I	GATATC	1 cut(s)	753
Eco47I	GGWCC	2 cut(s)	854, 1535
Eco47III	AGCGCT	1 cut(s)	1107
Eco53kI	GAGCTC	1 cut(s)	105
Eco57I	CTGAAG	2 cut(s)	438, 1148
Eco81I	CCTNAGG	1 cut(s)	1074
EcoICRI	GAGCTC	1 cut(s)	105
EcoO109I	RGGNCCY	1 cut(s)	1535
EcoRI	GAATTC	1 cut(s)	1122
EcoRII	CCWGG	3 cut(s)	897, 1182, 1305
EcoRV	GATATC	1 cut(s)	753
EcoT14I	CCWWGG	4 cut(s)	249, 568, 1117, 1275
EcoT22I	ATGCAT	1 cut(s)	412
EcoT38I	GRGCYC	1 cut(s)	107
ErhI	CCWWGG	4 cut(s)	249, 568, 1117, 1275
FaeI	CATG	6 cut(s)	225, 253, 610, 988, 1279, 1363
FalI	AAGNNNNNCTT	6 cut(s)	143, 175, 1092, 1124, 1400, 1432
FaqI	GGGAC	5 cut(s)	214, 811, 915, 946, 1014
FatI	CATG	6 cut(s)	221, 249, 606, 984, 1275, 1359
FauI	CCCGC	1 cut(s)	1450
FbaI	TGATCA	1 cut(s)	498
FokI	GGATG	7 cut(s)	254, 850, 865, 1030, 1199, 1294, 1414
FriOI	GRGCYC	1 cut(s)	107
FspBI	CTAG	2 cut(s)	629, 1364
GlaI	GCGC	1 cut(s)	1107
GsuI	CTGGAG	1 cut(s)	68
HaeII	RGCGCY	1 cut(s)	1109
HaeIII	GGCC	2 cut(s)	137, 305
HapII	CCGG	1 cut(s)	1383
HhaI	GCGC	1 cut(s)	1108
Hin1II	CATG	6 cut(s)	225, 253, 610, 988, 1279, 1363
Hin6I	GCGC	1 cut(s)	1106
HinP1I	GCGC	1 cut(s)	1106
HincII	GTYRAC	2 cut(s)	54, 94
HindII	GTYRAC	2 cut(s)	54, 94
HinfI	GANTC	8 cut(s)	5, 296, 476, 703, 1008, 1046, 1158, 1310
HpaII	CCGG	1 cut(s)	1383
HphI	GGTGA	4 cut(s)	725, 860, 1055, 1450
Hpy166II	GTNNAC	5 cut(s)	54, 94, 597, 702, 1414
Hpy188I	TCNGA	4 cut(s)	643, 750, 932, 1315
Hpy188III	TCNNGA	6 cut(s)	66, 239, 581, 1166, 1548, 1554
Hpy8I	GTNNAC	5 cut(s)	54, 94, 597, 702, 1414
HpyAV	CCTTC	4 cut(s)	316, 384, 766, 1172
HpyCH4III	ACNGT	5 cut(s)	205, 746, 760, 1040, 1525
HpyCH4IV	ACGT	2 cut(s)	593, 698
HpyF10VI	GCNNNNNNNGC	8 cut(s)	182, 191, 686, 1340, 1388, 1463, 1475, 1487
HpyF3I	CTNAG	6 cut(s)	13, 640, 1074, 1237, 1314, 1530
HpySE526I	ACGT	2 cut(s)	593, 698
Hsp92II	CATG	6 cut(s)	225, 253, 610, 988, 1279, 1363
HspAI	GCGC	1 cut(s)	1106
Ksp22I	TGATCA	1 cut(s)	498
Kzo9I	GATC	5 cut(s)	68, 76, 498, 1355, 1550
LmnI	GCTCC	4 cut(s)	376, 898, 932, 1103
LweI	GCATC	5 cut(s)	419, 887, 1008, 1018, 1177
MaeI	CTAG	2 cut(s)	629, 1364
MaeII	ACGT	2 cut(s)	593, 698
MaeIII	GTNAC	6 cut(s)	42, 140, 399, 556, 718, 1083
MalI	GATC	5 cut(s)	70, 78, 500, 1357, 1552
MboI	GATC	5 cut(s)	68, 76, 498, 1355, 1550
MboII	GAAGA	4 cut(s)	305, 556, 1105, 1411
MfeI	CAATTG	2 cut(s)	108, 459
MflI	RGATCY	3 cut(s)	68, 1355, 1550
MhlI	GDGCHC	2 cut(s)	107, 373
MlyI	GAGTC	4 cut(s)	305, 697, 1017, 1304
Mph1103I	ATGCAT	1 cut(s)	412
MseI	TTAA	1 cut(s)	213
MslI	CAYNNNNRTG	4 cut(s)	605, 989, 1041, 1280
MspA1I	CMGCKG	2 cut(s)	191, 1327
MspI	CCGG	1 cut(s)	1383
MspR9I	CCNGG	3 cut(s)	899, 1184, 1307
MunI	CAATTG	2 cut(s)	108, 459
MvaI	CCWGG	3 cut(s)	899, 1184, 1307
MwoI	GCNNNNNNNGC	8 cut(s)	182, 191, 686, 1340, 1388, 1463, 1475, 1487
NcoI	CCATGG	2 cut(s)	249, 1275
NdeII	GATC	5 cut(s)	68, 76, 498, 1355, 1550
NlaIII	CATG	6 cut(s)	225, 253, 610, 988, 1279, 1363
NlaIV	GGNNCC	5 cut(s)	70, 894, 1357, 1536, 1552
NmuCI	GTSAC	2 cut(s)	556, 1083
NsiI	ATGCAT	1 cut(s)	412
OliI	CACNNNNGTG	1 cut(s)	1041
PasI	CCCWGGG	1 cut(s)	898
PfeI	GAWTC	4 cut(s)	5, 476, 1046, 1158
PflMI	CCANNNNNTGG	1 cut(s)	65
PleI	GAGTC	4 cut(s)	304, 697, 1016, 1304
PpsI	GAGTC	4 cut(s)	304, 697, 1016, 1304
Ppu21I	YACGTR	1 cut(s)	699
PpuMI	RGGWCCY	1 cut(s)	1535
Psp124BI	GAGCTC	1 cut(s)	107
Psp5II	RGGWCCY	1 cut(s)	1535
Psp6I	CCWGG	3 cut(s)	897, 1182, 1305
PspGI	CCWGG	3 cut(s)	897, 1182, 1305
PspN4I	GGNNCC	5 cut(s)	70, 894, 1357, 1536, 1552
PspPI	GGNCC	2 cut(s)	854, 1535
PspPPI	RGGWCCY	1 cut(s)	1535
PstI	CTGCAG	3 cut(s)	190, 1273, 1381
PsuI	RGATCY	3 cut(s)	68, 1355, 1550
PvuII	CAGCTG	2 cut(s)	191, 1327
RsaI	GTAC	6 cut(s)	598, 697, 1071, 1286, 1348, 1427
RsaNI	GTAC	6 cut(s)	597, 696, 1070, 1285, 1347, 1426
RseI	CAYNNNNRTG	4 cut(s)	605, 989, 1041, 1280
SacI	GAGCTC	1 cut(s)	107
SaqAI	TTAA	1 cut(s)	213
Sau3AI	GATC	5 cut(s)	68, 76, 498, 1355, 1550
Sau96I	GGNCC	2 cut(s)	854, 1535
SchI	GAGTC	4 cut(s)	305, 697, 1017, 1304
ScrFI	CCNGG	3 cut(s)	899, 1184, 1307
SduI	GDGCHC	2 cut(s)	107, 373
SfaNI	GCATC	5 cut(s)	419, 887, 1008, 1018, 1177
SfcI	CTRYAG	4 cut(s)	171, 186, 1269, 1377
SinI	GGWCC	2 cut(s)	854, 1535
SmiMI	CAYNNNNRTG	4 cut(s)	605, 989, 1041, 1280
SmlI	CTYRAG	1 cut(s)	1098
SmoI	CTYRAG	1 cut(s)	1098
SpeI	ACTAGT	1 cut(s)	628
SsiI	CCGC	3 cut(s)	649, 1151, 1457
SspI	AATATT	2 cut(s)	180, 1204
SspMI	CTAG	2 cut(s)	629, 1364
SstI	GAGCTC	1 cut(s)	107
StyD4I	CCNGG	3 cut(s)	897, 1182, 1305
StyI	CCWWGG	4 cut(s)	249, 568, 1117, 1275
TaaI	ACNGT	5 cut(s)	205, 746, 760, 1040, 1525
TaiI	ACGT	2 cut(s)	596, 701
TaqI	TCGA	1 cut(s)	520
TauI	GCSGC	1 cut(s)	1154
TfiI	GAWTC	4 cut(s)	5, 476, 1046, 1158
Tru1I	TTAA	1 cut(s)	213
Tru9I	TTAA	1 cut(s)	213
TscAI	CASTG	7 cut(s)	261, 430, 508, 765, 819, 1043, 1266
TseFI	GTSAC	2 cut(s)	556, 1083
Tsp45I	GTSAC	2 cut(s)	556, 1083
TspDTI	ATGAA	1 cut(s)	785
TspGWI	ACGGA	1 cut(s)	1102
TspRI	CASTG	7 cut(s)	261, 430, 508, 765, 819, 1043, 1266
Van91I	CCANNNNNTGG	1 cut(s)	65
VpaK11BI	GGWCC	2 cut(s)	854, 1535
XapI	RAATTY	1 cut(s)	1122
XspI	CTAG	2 cut(s)	629, 1364
Zsp2I	ATGCAT	1 cut(s)	412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.