RLG00000022405

B3 domain-containing transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014501
Physical Location & Seq
Forward (+)
83142875 .. 83145911
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022405

Sequence Viewer

Length: 993 bp
ATGATGATGGACCAAACCCACGCCGCCCTTCACAGCAAAACCGAGGCCTTTGCCTTGAGGGCAAGTGTTAACGAGCTCGGCTTTCTCACTCGTCATGATGATCAGGAGGTTGACAACAACAGCAAGAAGAAGCACGACAACAACGTCAACTCCTTTCACGGGTCGGATCGTCCCGGGTCGATCCGTGACCTTGTTCGTGCCGTCTCAACTTTTGGTGTGCAGAGGAAGAAACGGATGATGAGGCAGAGGCGAGCCACCACCATCAACTTTCAGCTCTCTTTTGCCACCCCTAGCTCTCACGTGCTCCCTTCCACTTCCCCACCTGCACGTGTAACTGATCCCACAAGGTTGAGATTTCTTTTCCAAAAGGAACTTAAGAACAGTGATGTGAGCACTCTCAGAAGGGTGGTACTCCCTAAGAAAGCTGCTGAGGCTCATCTCCCAGCCCTGGAGTCTAAGGAAGGGATTTCTATCAGCATGGATGACATAGATGGTCTGCATGTTTGGAGCTTTAAGTACAGATTTTGGCCTAACAACAACAGCCGCATGTATGTGCTCGAGAATACTGGGGAATTCGTTAGCACACATGGCTTGCAACTTGGAGATTTCATCATGGTTTATCAAGATAGTGCAAACCACAATTATGTCATTCAGGCTAAAAAGGCCAACTCTGACCAAGATGATGATGAAGAAGATGATACTGTATATGGAGTGGAAGAAACTATGAAAAATGCTGTGGATGAGCTCATGATTTATGACTCGGTCCCTGATCAGGCTAACAAGTCTAGCTCGTTTTACATGCCGACAATGGATCATGATCATATGCAGGATGTTAATGCAGGCATGTCATTTGTGTATGACACCACTGCCTTCTCAAACGACTCTCTCCTTGATTTTTTGGGTGGATCCATGACCAACTATTCCAGATCAAATGGACGTCTACAGGAAAATTTTGGGTCTGTTGAGAACCTGTCTCTTGATGACTTCTATTAA

Protein Analysis

331

Amino Acids

37.47

Weight (kDa)

5.44

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 121 - 218 1.4e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26790
fragaria_vesca FvH4_6g54170
malus_domestica MD09G1004700.v1.1 MD17G1008700.v1.1
prunus_persica Prupe.3G312100_v2.0.a1
pyrus_communis pycom17g00360
rosa_chinensis RchiOBHm_Chr2g0176341
rosa_laevigata RLG00000022405
rosa_multiflora Rmu_co8325273.1_g000001 Rmu_sc0027060.1_g000003
rosa_roxburghii Rroxscaffold_2G00076550
rosa_rugosa Rorug02G0593400
rosa_samantha Rh2AG671900 Rh2BG683400 Rh2CG646100 Rh2DG696900
rosa_wichuraiana Rw2G055100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 331
AasI GACNNNNNNGTC 1 cut(s) 143
AatII GACGTC 1 cut(s) 940
Acc36I ACCTGC 1 cut(s) 331
AccI GTMKAC 1 cut(s) 940
AciI CCGC 2 cut(s) 24, 544
AclWI GGATC 6 cut(s) 174, 175, 332, 819, 900, 913
AcsI RAATTY 2 cut(s) 572, 949
AcvI CACGTG 2 cut(s) 301, 329
AcyI GRCGYC 1 cut(s) 937
AfaI GTAC 2 cut(s) 411, 518
AfiI CCNNNNNNNGG 3 cut(s) 159, 448, 772
AflII CTTAAG 1 cut(s) 374
AflIII ACRYGT 1 cut(s) 328
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 7 cut(s) 76, 274, 294, 425, 510, 745, 789
AluI AGCT 7 cut(s) 76, 274, 294, 425, 510, 745, 789
Alw21I GWGCWC 5 cut(s) 78, 306, 395, 558, 747
Alw26I GTCTC 2 cut(s) 208, 978
AlwI GGATC 6 cut(s) 174, 175, 332, 819, 900, 913
Ama87I CYCGRG 2 cut(s) 173, 557
AoxI GGCC 3 cut(s) 45, 527, 663
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 2 cut(s) 572, 949
AspS9I GGNCC 2 cut(s) 10, 763
AsuC2I CCSGG 2 cut(s) 174, 175
AvaI CYCGRG 2 cut(s) 173, 557
AvaII GGWCC 2 cut(s) 10, 763
BamHI GGATCC 1 cut(s) 905
BanII GRGCYC 2 cut(s) 78, 747
BbrPI CACGTG 2 cut(s) 301, 329
Bbv12I GWGCWC 5 cut(s) 78, 306, 395, 558, 747
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 269, 485
BceAI ACGGC 1 cut(s) 185
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BciT130I CCWGG 1 cut(s) 449
BclI TGATCA 3 cut(s) 100, 769, 817
BcnI CCSGG 2 cut(s) 174, 175
BcoDI GTCTC 2 cut(s) 208, 978
BfaI CTAG 2 cut(s) 291, 786
BfmI CTRYAG 1 cut(s) 941
BfrI CTTAAG 1 cut(s) 374
BfuAI ACCTGC 1 cut(s) 331
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 3 cut(s) 24, 426, 544
BlsI GCNGC 3 cut(s) 25, 427, 545
Bme1390I CCNGG 3 cut(s) 174, 175, 449
Bme18I GGWCC 2 cut(s) 10, 763
BmeT110I CYCGRG 2 cut(s) 173, 557
BmgT120I GGNCC 2 cut(s) 10, 763
BmiI GGNNCC 2 cut(s) 765, 907
BmrFI CCNGG 3 cut(s) 174, 175, 449
BmrI ACTGGG 1 cut(s) 576
BmuI ACTGGG 1 cut(s) 576
BpmI CTGGAG 1 cut(s) 470
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 76
BpuMI CCSGG 2 cut(s) 174, 175
BsaAI YACGTR 2 cut(s) 301, 329
BsaBI GATNNNNATC 2 cut(s) 470, 816
BsaHI GRCGYC 1 cut(s) 937
BsaJI CCNNGG 3 cut(s) 42, 173, 447
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 448, 772
Bse1I ACTGG 1 cut(s) 571
Bse8I GATNNNNATC 2 cut(s) 470, 816
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 3 cut(s) 42, 173, 447
BseGI GGATG 4 cut(s) 240, 487, 745, 835
BseJI GATNNNNATC 2 cut(s) 470, 816
BseLI CCNNNNNNNGG 3 cut(s) 159, 448, 772
BseMII CTCAG 2 cut(s) 412, 420
BseNI ACTGG 1 cut(s) 571
BseXI GCAGC 1 cut(s) 412
BseYI CCCAGC 1 cut(s) 442
BsgI GTGCAG 2 cut(s) 239, 309
BshFI GGCC 3 cut(s) 47, 529, 665
BsiHKAI GWGCWC 5 cut(s) 78, 306, 395, 558, 747
BsiHKCI CYCGRG 2 cut(s) 173, 557
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 2 cut(s) 156, 749
BslI CCNNNNNNNGG 3 cut(s) 159, 448, 772
BsmAI GTCTC 2 cut(s) 208, 978
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 2 cut(s) 156, 749
BsnI GGCC 3 cut(s) 47, 529, 665
BsoBI CYCGRG 2 cut(s) 173, 557
Bsp1286I GDGCHC 5 cut(s) 78, 306, 395, 558, 747
Bsp143I GATC 9 cut(s) 100, 166, 180, 337, 769, 811, 817, 905, 926
BspACI CCGC 2 cut(s) 24, 544
BspANI GGCC 3 cut(s) 47, 529, 665
BspCNI CTCAG 2 cut(s) 411, 421
BspHI TCATGA 3 cut(s) 94, 747, 814
BspLI GGNNCC 2 cut(s) 765, 907
BspMI ACCTGC 1 cut(s) 331
BspPI GGATC 6 cut(s) 174, 175, 332, 819, 900, 913
BspTI CTTAAG 1 cut(s) 374
BsrI ACTGG 1 cut(s) 571
BssECI CCNNGG 3 cut(s) 42, 173, 447
BssMI GATC 9 cut(s) 100, 166, 180, 337, 769, 811, 817, 905, 926
BssNI GRCGYC 1 cut(s) 937
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 2 cut(s) 383, 703
BstACI GRCGYC 1 cut(s) 937
BstAFI CTTAAG 1 cut(s) 374
BstBAI YACGTR 2 cut(s) 301, 329
BstC8I GCNNGC 3 cut(s) 252, 593, 841
BstDEI CTNAG 4 cut(s) 398, 417, 429, 456
BstF5I GGATG 4 cut(s) 240, 487, 745, 835
BstKTI GATC 9 cut(s) 103, 169, 183, 340, 772, 814, 820, 908, 929
BstMAI GTCTC 2 cut(s) 208, 978
BstMBI GATC 9 cut(s) 100, 166, 180, 337, 769, 811, 817, 905, 926
BstMWI GCNNNNNNNGC 4 cut(s) 59, 431, 588, 662
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 4 cut(s) 503, 550, 802, 847
BstSCI CCNGG 3 cut(s) 172, 173, 447
BstSFI CTRYAG 1 cut(s) 941
BstV1I GCAGC 1 cut(s) 412
BstX2I RGATCY 1 cut(s) 905
BstYI RGATCY 1 cut(s) 905
BsuRI GGCC 3 cut(s) 47, 529, 665
BtsCI GGATG 4 cut(s) 240, 487, 745, 835
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 2 cut(s) 388, 864
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 3 cut(s) 252, 593, 841
CciI TCATGA 3 cut(s) 94, 747, 814
Cfr13I GGNCC 2 cut(s) 10, 763
Cfr9I CCCGGG 1 cut(s) 173
Csp6I GTAC 2 cut(s) 410, 517
CviQI GTAC 2 cut(s) 410, 517
DdeI CTNAG 4 cut(s) 398, 417, 429, 456
DpnI GATC 9 cut(s) 102, 168, 182, 339, 771, 813, 819, 907, 928
DpnII GATC 9 cut(s) 100, 166, 180, 337, 769, 811, 817, 905, 926
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
Ecl136II GAGCTC 2 cut(s) 76, 745
Eco147I AGGCCT 1 cut(s) 47
Eco24I GRGCYC 2 cut(s) 78, 747
Eco47I GGWCC 2 cut(s) 10, 763
Eco53kI GAGCTC 2 cut(s) 76, 745
Eco72I CACGTG 2 cut(s) 301, 329
Eco88I CYCGRG 2 cut(s) 173, 557
EcoICRI GAGCTC 2 cut(s) 76, 745
EcoRI GAATTC 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 447
EcoT38I GRGCYC 2 cut(s) 78, 747
Esp3I CGTCTC 1 cut(s) 208
FaqI GGGAC 2 cut(s) 156, 749
FauNDI CATATG 1 cut(s) 822
FbaI TGATCA 3 cut(s) 100, 769, 817
FblI GTMKAC 1 cut(s) 940
Fnu4HI GCNGC 3 cut(s) 24, 426, 544
FokI GGATG 4 cut(s) 247, 494, 752, 842
FriOI GRGCYC 2 cut(s) 78, 747
Fsp4HI GCNGC 3 cut(s) 24, 426, 544
FspBI CTAG 2 cut(s) 291, 786
GluI GCNGC 3 cut(s) 24, 426, 544
GsaI CCCAGC 1 cut(s) 446
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 3 cut(s) 47, 529, 665
HapII CCGG 1 cut(s) 174
Hin1I GRCGYC 1 cut(s) 937
HincII GTYRAC 3 cut(s) 70, 112, 148
HindII GTYRAC 3 cut(s) 70, 112, 148
HinfI GANTC 3 cut(s) 452, 758, 881
HpaI GTTAAC 1 cut(s) 70
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 4 cut(s) 70, 112, 148, 941
Hpy188I TCNGA 3 cut(s) 166, 401, 673
Hpy188III TCNNGA 8 cut(s) 95, 104, 559, 623, 748, 815, 924, 977
Hpy8I GTNNAC 4 cut(s) 70, 112, 148, 941
HpyAV CCTTC 5 cut(s) 38, 318, 396, 455, 880
HpyCH4III ACNGT 2 cut(s) 383, 703
HpyCH4IV ACGT 4 cut(s) 144, 300, 328, 937
HpyCH4V TGCA 7 cut(s) 220, 326, 499, 595, 632, 826, 839
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 431, 588, 662
HpyF3I CTNAG 4 cut(s) 398, 417, 429, 456
HpySE526I ACGT 4 cut(s) 144, 300, 328, 937
Hsp92I GRCGYC 1 cut(s) 937
Ksp22I TGATCA 3 cut(s) 100, 769, 817
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 9 cut(s) 100, 166, 180, 337, 769, 811, 817, 905, 926
LmnI GCTCC 2 cut(s) 309, 507
Lsp1109I GCAGC 1 cut(s) 412
MaeI CTAG 2 cut(s) 291, 786
MaeII ACGT 4 cut(s) 144, 300, 328, 937
MaeIII GTNAC 2 cut(s) 185, 331
MalI GATC 9 cut(s) 102, 168, 182, 339, 771, 813, 819, 907, 928
MboI GATC 9 cut(s) 100, 166, 180, 337, 769, 811, 817, 905, 926
MboII GAAGA 5 cut(s) 139, 238, 701, 704, 728
MflI RGATCY 1 cut(s) 905
MhlI GDGCHC 5 cut(s) 78, 306, 395, 558, 747
MluCI AATT 3 cut(s) 572, 640, 949
MlyI GAGTC 3 cut(s) 461, 752, 875
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 7 cut(s) 37, 51, 100, 216, 234, 240, 424
MseI TTAA 5 cut(s) 69, 375, 513, 834, 991
MslI CAYNNNNRTG 2 cut(s) 551, 642
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 3 cut(s) 174, 175, 449
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 4 cut(s) 59, 431, 588, 662
NciI CCSGG 2 cut(s) 174, 175
NdeI CATATG 1 cut(s) 822
NdeII GATC 9 cut(s) 100, 166, 180, 337, 769, 811, 817, 905, 926
NlaIV GGNNCC 2 cut(s) 765, 907
NmeAIII GCCGAG 1 cut(s) 57
NmuCI GTSAC 1 cut(s) 185
NspI RCATGY 4 cut(s) 503, 550, 802, 847
PaeR7I CTCGAG 1 cut(s) 557
PagI TCATGA 3 cut(s) 94, 747, 814
PaqCI CACCTGC 1 cut(s) 331
PceI AGGCCT 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PflFI GACNNNGTC 1 cut(s) 761
PkrI GCNGC 3 cut(s) 25, 427, 545
PleI GAGTC 3 cut(s) 460, 752, 875
PmaCI CACGTG 2 cut(s) 301, 329
PmlI CACGTG 2 cut(s) 301, 329
PpsI GAGTC 3 cut(s) 460, 752, 875
Ppu21I YACGTR 2 cut(s) 301, 329
Psp124BI GAGCTC 2 cut(s) 78, 747
Psp6I CCWGG 1 cut(s) 447
PspCI CACGTG 2 cut(s) 301, 329
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 2 cut(s) 765, 907
PspPI GGNCC 2 cut(s) 10, 763
PsuI RGATCY 1 cut(s) 905
PsyI GACNNNGTC 1 cut(s) 761
RsaI GTAC 2 cut(s) 411, 518
RsaNI GTAC 2 cut(s) 410, 517
RseI CAYNNNNRTG 2 cut(s) 551, 642
SacI GAGCTC 2 cut(s) 78, 747
SaqAI TTAA 5 cut(s) 69, 375, 513, 834, 991
SatI GCNGC 3 cut(s) 24, 426, 544
Sau3AI GATC 9 cut(s) 100, 166, 180, 337, 769, 811, 817, 905, 926
Sau96I GGNCC 2 cut(s) 10, 763
SchI GAGTC 3 cut(s) 461, 752, 875
ScrFI CCNGG 3 cut(s) 174, 175, 449
SduI GDGCHC 5 cut(s) 78, 306, 395, 558, 747
SfcI CTRYAG 1 cut(s) 941
Sfr274I CTCGAG 1 cut(s) 557
SinI GGWCC 2 cut(s) 10, 763
SlaI CTCGAG 1 cut(s) 557
SmaI CCCGGG 1 cut(s) 175
SmiMI CAYNNNNRTG 2 cut(s) 551, 642
SmlI CTYRAG 3 cut(s) 55, 374, 557
SmoI CTYRAG 3 cut(s) 55, 374, 557
Sse9I AATT 3 cut(s) 572, 640, 949
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 2 cut(s) 24, 544
SspMI CTAG 2 cut(s) 291, 786
SstI GAGCTC 2 cut(s) 78, 747
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 172, 173, 447
TaaI ACNGT 2 cut(s) 383, 703
TaiI ACGT 4 cut(s) 147, 303, 331, 940
TaqI TCGA 2 cut(s) 179, 558
TaqII GACCGA 1 cut(s) 751
TasI AATT 3 cut(s) 572, 640, 949
TatI WGTACW 1 cut(s) 516
TauI GCSGC 2 cut(s) 26, 546
Tru1I TTAA 5 cut(s) 69, 375, 513, 834, 991
Tru9I TTAA 5 cut(s) 69, 375, 513, 834, 991
TscAI CASTG 2 cut(s) 388, 871
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 3 cut(s) 598, 702, 740
TspGWI ACGGA 2 cut(s) 173, 247
TspMI CCCGGG 1 cut(s) 173
TspRI CASTG 2 cut(s) 388, 871
Tth111I GACNNNGTC 1 cut(s) 761
Vha464I CTTAAG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 10, 763
XapI RAATTY 2 cut(s) 572, 949
XceI RCATGY 4 cut(s) 503, 550, 802, 847
XhoI CTCGAG 1 cut(s) 557
XmaI CCCGGG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 940
XspI CTAG 2 cut(s) 291, 786
ZraI GACGTC 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.