Rh2AG671900

B3 domain-containing transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014501
Physical Location & Seq
Forward (+)
88488905 .. 88492950
4046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG671900.1

Sequence Viewer

Length: 978 bp
ATGATGATGGACCAAACCCACGCCGCCCTTCACAGCAAAACCGAGGCCTTTGCCTTGAGGGCAGGTGTTAACGAGCTCGGCTTTCTCACTCGTCATGATGATCAGGAGGTTGACAACAACAACAACAACGTCAACTCCTTTCACGGGTCGGATCGTCCCGGGTCGATCCGTGACCTTGTTCGTGCCGTCTCAACTTTTGGTGTGCAGAGGAAGAAACGGATGATGAGGCAGAGGCGAGCCACCACCATCAACTTTCAGCTCTCTTTTGCCACCCCCAGCTCTCACGTGCTCCCTTCCACTTCCCCACCTGCACGTGTAACTGATCCTGCAAGGTTGAGATTTCTTTTCCAAAAGGAACTTAAGAACAGTGATGTGAGCACTCTCAGAAGGGTGGTACTCCCTAAGAAAGCAGCTGAGGCTCATCTCCCAGCCCTGGAGTCTAAGGAAGGGATTTCTATCAGCATGGATGACATAGATGGTCTGCATGTTTGGAGCTTTAAGTACAGATTTTGGCCTAACAACAACAGCCGCATGTATGTGCTCGAGAATACTGGGGAATTCGTTAGCACACATGGCTTGCAACTTGGAGATTTCATCATGGTTTATCAAGATAGTGCAAACCACAATTATGTCATTCAGGCTAAAAAGGCCAACTCTGACCAAGATGATGATGATGAAGATGAAACTGTATATGGAGTGGAAGAAACTATGAAGAATGCTGTGAATGAGCTCATGATTTATGACTCGGTCCCTGATCAGGCTAACAAGTCTAGCTCGTTTTACATGCCGACAATGGATCATGATCATATGCAGGATGTTAATGCAGGCATGTCATTTGTGTATGACACCACTGCCTTCTCAAACGACTCTCTCCTTGATTTTTTGGGTGGATCCATGACCAACTATTCCAGATCAAATGGACGTCTGCAGGAAAATTTTGGGTCTGTTGAGAACCTGTCTCTTGATGACTTCTATTAA

Protein Analysis

325

Amino Acids

36.8

Weight (kDa)

5.27

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 116 - 213 1.3e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26790
fragaria_vesca FvH4_6g54170
malus_domestica MD09G1004700.v1.1 MD17G1008700.v1.1
prunus_persica Prupe.3G312100_v2.0.a1
pyrus_communis pycom17g00360
rosa_chinensis RchiOBHm_Chr2g0176341
rosa_laevigata RLG00000022405
rosa_multiflora Rmu_co8325273.1_g000001 Rmu_sc0027060.1_g000003
rosa_roxburghii Rroxscaffold_2G00076550
rosa_rugosa Rorug02G0593400
rosa_samantha Rh2AG671900 Rh2BG683400 Rh2CG646100 Rh2DG696900
rosa_wichuraiana Rw2G055100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 53, 316
AatII GACGTC 1 cut(s) 925
Acc36I ACCTGC 2 cut(s) 53, 316
AciI CCGC 2 cut(s) 24, 529
AclWI GGATC 6 cut(s) 159, 160, 317, 804, 885, 898
AcsI RAATTY 2 cut(s) 557, 934
AcvI CACGTG 2 cut(s) 286, 314
AcyI GRCGYC 1 cut(s) 922
AfaI GTAC 2 cut(s) 396, 503
AfiI CCNNNNNNNGG 3 cut(s) 144, 433, 757
AflII CTTAAG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 313
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 7 cut(s) 76, 259, 279, 413, 495, 730, 774
AluI AGCT 7 cut(s) 76, 259, 279, 413, 495, 730, 774
Alw21I GWGCWC 5 cut(s) 78, 291, 380, 543, 732
Alw26I GTCTC 2 cut(s) 193, 963
AlwI GGATC 6 cut(s) 159, 160, 317, 804, 885, 898
Ama87I CYCGRG 2 cut(s) 158, 542
AoxI GGCC 3 cut(s) 45, 512, 648
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 2 cut(s) 557, 934
AspS9I GGNCC 2 cut(s) 10, 748
AsuC2I CCSGG 2 cut(s) 159, 160
AvaI CYCGRG 2 cut(s) 158, 542
AvaII GGWCC 2 cut(s) 10, 748
BamHI GGATCC 1 cut(s) 890
BanII GRGCYC 2 cut(s) 78, 732
BbrPI CACGTG 2 cut(s) 286, 314
Bbv12I GWGCWC 5 cut(s) 78, 291, 380, 543, 732
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 1 cut(s) 422
BccI CCATC 2 cut(s) 254, 470
BceAI ACGGC 1 cut(s) 170
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BciT130I CCWGG 1 cut(s) 434
BclI TGATCA 3 cut(s) 100, 754, 802
BcnI CCSGG 2 cut(s) 159, 160
BcoDI GTCTC 2 cut(s) 193, 963
BfaI CTAG 1 cut(s) 771
BfmI CTRYAG 1 cut(s) 926
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 2 cut(s) 53, 316
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 3 cut(s) 24, 411, 529
BlsI GCNGC 3 cut(s) 25, 412, 530
Bme1390I CCNGG 3 cut(s) 159, 160, 434
Bme18I GGWCC 2 cut(s) 10, 748
BmeT110I CYCGRG 2 cut(s) 158, 542
BmgT120I GGNCC 2 cut(s) 10, 748
BmiI GGNNCC 2 cut(s) 750, 892
BmrFI CCNGG 3 cut(s) 159, 160, 434
BmrI ACTGGG 1 cut(s) 561
BmuI ACTGGG 1 cut(s) 561
BpmI CTGGAG 1 cut(s) 455
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 76
BpuMI CCSGG 2 cut(s) 159, 160
BsaAI YACGTR 2 cut(s) 286, 314
BsaBI GATNNNNATC 2 cut(s) 455, 801
BsaHI GRCGYC 1 cut(s) 922
BsaJI CCNNGG 3 cut(s) 42, 158, 432
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 433, 757
Bse1I ACTGG 1 cut(s) 556
Bse8I GATNNNNATC 2 cut(s) 455, 801
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 3 cut(s) 42, 158, 432
BseGI GGATG 3 cut(s) 225, 472, 820
BseJI GATNNNNATC 2 cut(s) 455, 801
BseLI CCNNNNNNNGG 3 cut(s) 144, 433, 757
BseMII CTCAG 2 cut(s) 397, 405
BseNI ACTGG 1 cut(s) 556
BseXI GCAGC 1 cut(s) 422
BseYI CCCAGC 2 cut(s) 275, 427
BsgI GTGCAG 2 cut(s) 224, 294
BshFI GGCC 3 cut(s) 47, 514, 650
BsiHKAI GWGCWC 5 cut(s) 78, 291, 380, 543, 732
BsiHKCI CYCGRG 2 cut(s) 158, 542
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 2 cut(s) 141, 734
BslI CCNNNNNNNGG 3 cut(s) 144, 433, 757
BsmAI GTCTC 2 cut(s) 193, 963
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 2 cut(s) 141, 734
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 3 cut(s) 47, 514, 650
BsoBI CYCGRG 2 cut(s) 158, 542
Bsp1286I GDGCHC 5 cut(s) 78, 291, 380, 543, 732
Bsp143I GATC 9 cut(s) 100, 151, 165, 322, 754, 796, 802, 890, 911
BspACI CCGC 2 cut(s) 24, 529
BspANI GGCC 3 cut(s) 47, 514, 650
BspCNI CTCAG 2 cut(s) 396, 406
BspHI TCATGA 3 cut(s) 94, 732, 799
BspLI GGNNCC 2 cut(s) 750, 892
BspMAI CTGCAG 1 cut(s) 930
BspMI ACCTGC 2 cut(s) 53, 316
BspPI GGATC 6 cut(s) 159, 160, 317, 804, 885, 898
BspTI CTTAAG 1 cut(s) 359
BsrI ACTGG 1 cut(s) 556
BssECI CCNNGG 3 cut(s) 42, 158, 432
BssMI GATC 9 cut(s) 100, 151, 165, 322, 754, 796, 802, 890, 911
BssNI GRCGYC 1 cut(s) 922
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 2 cut(s) 368, 688
BstACI GRCGYC 1 cut(s) 922
BstAFI CTTAAG 1 cut(s) 359
BstBAI YACGTR 2 cut(s) 286, 314
BstC8I GCNNGC 3 cut(s) 237, 578, 826
BstDEI CTNAG 4 cut(s) 383, 402, 414, 441
BstF5I GGATG 3 cut(s) 225, 472, 820
BstKTI GATC 9 cut(s) 103, 154, 168, 325, 757, 799, 805, 893, 914
BstMAI GTCTC 2 cut(s) 193, 963
BstMBI GATC 9 cut(s) 100, 151, 165, 322, 754, 796, 802, 890, 911
BstMWI GCNNNNNNNGC 4 cut(s) 59, 416, 573, 647
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 4 cut(s) 488, 535, 787, 832
BstSCI CCNGG 3 cut(s) 157, 158, 432
BstSFI CTRYAG 1 cut(s) 926
BstV1I GCAGC 1 cut(s) 422
BstX2I RGATCY 1 cut(s) 890
BstYI RGATCY 1 cut(s) 890
BsuRI GGCC 3 cut(s) 47, 514, 650
BtsCI GGATG 3 cut(s) 225, 472, 820
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 2 cut(s) 373, 849
BveI ACCTGC 2 cut(s) 53, 316
Cac8I GCNNGC 3 cut(s) 237, 578, 826
CciI TCATGA 3 cut(s) 94, 732, 799
Cfr13I GGNCC 2 cut(s) 10, 748
Cfr9I CCCGGG 1 cut(s) 158
Csp6I GTAC 2 cut(s) 395, 502
CviQI GTAC 2 cut(s) 395, 502
DdeI CTNAG 4 cut(s) 383, 402, 414, 441
DpnI GATC 9 cut(s) 102, 153, 167, 324, 756, 798, 804, 892, 913
DpnII GATC 9 cut(s) 100, 151, 165, 322, 754, 796, 802, 890, 911
Ecl136II GAGCTC 2 cut(s) 76, 730
Eco147I AGGCCT 1 cut(s) 47
Eco24I GRGCYC 2 cut(s) 78, 732
Eco47I GGWCC 2 cut(s) 10, 748
Eco53kI GAGCTC 2 cut(s) 76, 730
Eco72I CACGTG 2 cut(s) 286, 314
Eco88I CYCGRG 2 cut(s) 158, 542
EcoICRI GAGCTC 2 cut(s) 76, 730
EcoRI GAATTC 1 cut(s) 557
EcoRII CCWGG 1 cut(s) 432
EcoT38I GRGCYC 2 cut(s) 78, 732
Esp3I CGTCTC 1 cut(s) 193
FaqI GGGAC 2 cut(s) 141, 734
FauNDI CATATG 1 cut(s) 807
FbaI TGATCA 3 cut(s) 100, 754, 802
Fnu4HI GCNGC 3 cut(s) 24, 411, 529
FokI GGATG 3 cut(s) 232, 479, 827
FriOI GRGCYC 2 cut(s) 78, 732
Fsp4HI GCNGC 3 cut(s) 24, 411, 529
FspBI CTAG 1 cut(s) 771
GluI GCNGC 3 cut(s) 24, 411, 529
GsaI CCCAGC 2 cut(s) 279, 431
GsuI CTGGAG 1 cut(s) 455
HaeIII GGCC 3 cut(s) 47, 514, 650
HapII CCGG 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 922
HincII GTYRAC 3 cut(s) 70, 112, 133
HindII GTYRAC 3 cut(s) 70, 112, 133
HinfI GANTC 3 cut(s) 437, 743, 866
HpaI GTTAAC 1 cut(s) 70
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 3 cut(s) 70, 112, 133
Hpy188I TCNGA 3 cut(s) 151, 386, 658
Hpy188III TCNNGA 8 cut(s) 95, 104, 544, 608, 733, 800, 909, 962
Hpy8I GTNNAC 3 cut(s) 70, 112, 133
HpyAV CCTTC 5 cut(s) 38, 303, 381, 440, 865
HpyCH4III ACNGT 2 cut(s) 368, 688
HpyCH4IV ACGT 4 cut(s) 129, 285, 313, 922
HpyCH4V TGCA 9 cut(s) 205, 311, 329, 484, 580, 617, 811, 824, 928
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 416, 573, 647
HpyF3I CTNAG 4 cut(s) 383, 402, 414, 441
HpySE526I ACGT 4 cut(s) 129, 285, 313, 922
Hsp92I GRCGYC 1 cut(s) 922
Ksp22I TGATCA 3 cut(s) 100, 754, 802
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 9 cut(s) 100, 151, 165, 322, 754, 796, 802, 890, 911
LmnI GCTCC 2 cut(s) 294, 492
Lsp1109I GCAGC 1 cut(s) 422
MaeI CTAG 1 cut(s) 771
MaeII ACGT 4 cut(s) 129, 285, 313, 922
MaeIII GTNAC 2 cut(s) 170, 316
MalI GATC 9 cut(s) 102, 153, 167, 324, 756, 798, 804, 892, 913
MboI GATC 9 cut(s) 100, 151, 165, 322, 754, 796, 802, 890, 911
MboII GAAGA 4 cut(s) 223, 689, 713, 724
MflI RGATCY 1 cut(s) 890
MhlI GDGCHC 5 cut(s) 78, 291, 380, 543, 732
MluCI AATT 3 cut(s) 557, 625, 934
MlyI GAGTC 3 cut(s) 446, 737, 860
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 7 cut(s) 37, 51, 100, 201, 219, 225, 409
MseI TTAA 5 cut(s) 69, 360, 498, 819, 976
MslI CAYNNNNRTG 2 cut(s) 536, 627
MspA1I CMGCKG 1 cut(s) 413
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 3 cut(s) 159, 160, 434
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 1 cut(s) 434
MwoI GCNNNNNNNGC 4 cut(s) 59, 416, 573, 647
NciI CCSGG 2 cut(s) 159, 160
NdeI CATATG 1 cut(s) 807
NdeII GATC 9 cut(s) 100, 151, 165, 322, 754, 796, 802, 890, 911
NlaIV GGNNCC 2 cut(s) 750, 892
NmeAIII GCCGAG 1 cut(s) 57
NmuCI GTSAC 1 cut(s) 170
NspI RCATGY 4 cut(s) 488, 535, 787, 832
PaeR7I CTCGAG 1 cut(s) 542
PagI TCATGA 3 cut(s) 94, 732, 799
PaqCI CACCTGC 2 cut(s) 53, 316
PceI AGGCCT 1 cut(s) 47
PctI GAATGC 1 cut(s) 721
PflFI GACNNNGTC 1 cut(s) 746
PkrI GCNGC 3 cut(s) 25, 412, 530
PleI GAGTC 3 cut(s) 445, 737, 860
PmaCI CACGTG 2 cut(s) 286, 314
PmlI CACGTG 2 cut(s) 286, 314
PpsI GAGTC 3 cut(s) 445, 737, 860
Ppu21I YACGTR 2 cut(s) 286, 314
Psp124BI GAGCTC 2 cut(s) 78, 732
Psp6I CCWGG 1 cut(s) 432
PspCI CACGTG 2 cut(s) 286, 314
PspFI CCCAGC 2 cut(s) 275, 427
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 2 cut(s) 750, 892
PspPI GGNCC 2 cut(s) 10, 748
PstI CTGCAG 1 cut(s) 930
PsuI RGATCY 1 cut(s) 890
PsyI GACNNNGTC 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 2 cut(s) 396, 503
RsaNI GTAC 2 cut(s) 395, 502
RseI CAYNNNNRTG 2 cut(s) 536, 627
SacI GAGCTC 2 cut(s) 78, 732
SaqAI TTAA 5 cut(s) 69, 360, 498, 819, 976
SatI GCNGC 3 cut(s) 24, 411, 529
Sau3AI GATC 9 cut(s) 100, 151, 165, 322, 754, 796, 802, 890, 911
Sau96I GGNCC 2 cut(s) 10, 748
SchI GAGTC 3 cut(s) 446, 737, 860
ScrFI CCNGG 3 cut(s) 159, 160, 434
SduI GDGCHC 5 cut(s) 78, 291, 380, 543, 732
SfcI CTRYAG 1 cut(s) 926
Sfr274I CTCGAG 1 cut(s) 542
SinI GGWCC 2 cut(s) 10, 748
SlaI CTCGAG 1 cut(s) 542
SmaI CCCGGG 1 cut(s) 160
SmiMI CAYNNNNRTG 2 cut(s) 536, 627
SmlI CTYRAG 3 cut(s) 55, 359, 542
SmoI CTYRAG 3 cut(s) 55, 359, 542
Sse9I AATT 3 cut(s) 557, 625, 934
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 2 cut(s) 24, 529
SspMI CTAG 1 cut(s) 771
SstI GAGCTC 2 cut(s) 78, 732
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 157, 158, 432
TaaI ACNGT 2 cut(s) 368, 688
TaiI ACGT 4 cut(s) 132, 288, 316, 925
TaqI TCGA 2 cut(s) 164, 543
TaqII GACCGA 1 cut(s) 736
TasI AATT 3 cut(s) 557, 625, 934
TatI WGTACW 1 cut(s) 501
TauI GCSGC 2 cut(s) 26, 531
Tru1I TTAA 5 cut(s) 69, 360, 498, 819, 976
Tru9I TTAA 5 cut(s) 69, 360, 498, 819, 976
TscAI CASTG 2 cut(s) 373, 856
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 4 cut(s) 583, 690, 696, 725
TspGWI ACGGA 2 cut(s) 158, 232
TspMI CCCGGG 1 cut(s) 158
TspRI CASTG 2 cut(s) 373, 856
Tth111I GACNNNGTC 1 cut(s) 746
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 2 cut(s) 10, 748
XapI RAATTY 2 cut(s) 557, 934
XceI RCATGY 4 cut(s) 488, 535, 787, 832
XhoI CTCGAG 1 cut(s) 542
XmaI CCCGGG 1 cut(s) 158
XspI CTAG 1 cut(s) 771
ZraI GACGTC 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.