RLG00000028443
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012646
Physical Location & Seq
Reverse (-)
22924970 .. 22926751
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028443

Sequence Viewer

Length: 1524 bp
ATGTCGATTTCATCTTCTTCTTATTCAGGTGGGTTTCTCACGTCAAAGCCCTCAAATTCGATGGTGGCCTCCTCGTCTTCTTCTGGGACTGTGATGCCAACGCTGCCGTTAGGGTTTCTAAACCAATACAACCCAGATGAACTCTACTTCTTCATGCCTTCACTGAAGAAATTGAGCCCTAATGGTGGTTCAAGGATCAACCGAACAATTGGCAGTGGTAGTTGGACTCAGAAGGAGCCAATCAAGCTTGTGTGTGACGATGACGACGAGGGCAGATCAATTGGGGTGAAGAGAAAGCTTAGATATCACAACCGAGGGTCAAATCAACATGTGCAGTGGTGTTTGCATGAGTATACTAGTGTTGAGGTTGCTAATTCATGTGCAATTTTGCAGCTCCGGAAAAACCAATACAAATCACTATGTGGTATTTGTGTGGAAGACTTCATGCAGCCTTCACCATTAGGTATGAAGATTTTATTCCGCCCCACGGACTCCCAATTGATTAGGTACTTTCTTGGTTTTGATATTGCCCCAGATTTTATTGTTTATGATTGTGATTTGTTTGGTCAGGAAGAGCCTTGGGAAATTTGGGACAGGTATTCAGTGGTTAAAAACCACTCGGATCTCTTGTACTTTTTCACATCGTCTATGAAGAAACTGGGCTCCAAGGGGAGGGTTTGTAATTTTGGTCGAAGAGTTGGGTCTGGTACATGGAACCAAAAGGAGGCTGCCAAATTGGTGTATGATGATGAAGGCGAGGCCGATCAAGAAAGAAAGCCGATTGGGAGAAAGAGGAAACTTAGATATGTTAACCAAGGCTCTGTGCATCATGGGGTGTGGTATTTGGAAGAGTACACAAGCCTTGTTGATGAGGGGGCAATTTTGCTTCTTCGAAAAAACGAGCGGTACAAAGCACCGGCTTCATCAATGTTGTCAGGAATTCCAAGTGAAAAGAAGAGAAAACACAGAGAAATTGATCGATTGGAGGAGAAGAAGCCTAGAAAGAAAAAAGTTTCAAGACAACTAGTAATGCAGGACTCGGATTTACTTAATAGGCAAAGCCAAACTGCTACCAGTCCCACACTGCCTATTCAACAACAAGAACCTGACAATGGTGATGATAATTTCGTGTTCTTCAAGGATATTGATATTGGAACATGGGAGTTTCCTGCTCTGGATGATTTTGTCATGGCTTGTCCTCCTCCACTTACGGAATATGACTTGGCTTCTCCTTCTCCTCCTCTTGAGGACTGTGATCAACAAGTAATAGTAGCAGCCAACATTGATGATAATCATAATCTCATGGGATCTTGTAATGATGATGATATTGCAACATGGAGTTTTCCTATTGTGGATAGTTCATTGACTTCTCTTCTTCAAGGAAAATGTGATGAACAAGCATTAGCATTAGTCCACAGTGATGATGATTTACATTTCATGAAAGAGGATACTGGAGCATGGGGTCCTGCTTTTGTGGATGATTTCTTGATGGCTCTTCTTGTGGATAGTGATGAACAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

508

Amino Acids

57.28

Weight (kDa)

5.19

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 42 - 119 7.4e-10 No apical meristem (NAM) protein
NAM PF02365 159 - 286 6.1e-13 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 904
AccI GTMKAC 1 cut(s) 353
AccIII TCCGGA 1 cut(s) 396
AciI CCGC 2 cut(s) 481, 904
AclWI GGATC 3 cut(s) 203, 630, 1315
AcsI RAATTY 3 cut(s) 55, 585, 939
AcuI CTGAAG 1 cut(s) 185
AdeI CACNNNGTG 1 cut(s) 422
AfaI GTAC 5 cut(s) 509, 632, 709, 854, 908
AfiI CCNNNNNNNGG 5 cut(s) 185, 487, 672, 724, 1112
AflIII ACRYGT 1 cut(s) 328
AgsI TTSAA 5 cut(s) 192, 1017, 1094, 1138, 1379
AhlI ACTAGT 2 cut(s) 356, 1024
AjiI CACGTC 1 cut(s) 42
AluBI AGCT 3 cut(s) 247, 298, 394
AluI AGCT 3 cut(s) 247, 298, 394
AlwI GGATC 3 cut(s) 203, 630, 1315
Aor13HI TCCGGA 1 cut(s) 396
AoxI GGCC 2 cut(s) 66, 759
ApeKI GCWGC 5 cut(s) 103, 391, 448, 728, 1274
ApoI RAATTY 3 cut(s) 55, 585, 939
AspS9I GGNCC 1 cut(s) 1463
AsuHPI GGTGA 3 cut(s) 298, 447, 1127
AsuII TTCGAA 1 cut(s) 892
AvaII GGWCC 1 cut(s) 1463
BanII GRGCYC 2 cut(s) 179, 665
BbsI GAAGAC 2 cut(s) 69, 444
BbvI GCAGC 5 cut(s) 90, 403, 460, 715, 1286
BccI CCATC 2 cut(s) 55, 1483
BceAI ACGGC 1 cut(s) 91
BciVI GTATCC 1 cut(s) 1441
BclI TGATCA 1 cut(s) 1255
BcuI ACTAGT 2 cut(s) 356, 1024
BfaI CTAG 3 cut(s) 357, 999, 1025
BfuI GTATCC 1 cut(s) 1441
BisI GCNGC 5 cut(s) 104, 392, 449, 729, 1275
BlsI GCNGC 5 cut(s) 105, 393, 450, 730, 1276
Bme18I GGWCC 1 cut(s) 1463
BmgBI CACGTC 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 1463
BmiI GGNNCC 4 cut(s) 237, 664, 716, 1464
BmrI ACTGGG 1 cut(s) 668
BmsI GCATC 2 cut(s) 84, 835
BmuI ACTGGG 1 cut(s) 668
BpiI GAAGAC 2 cut(s) 69, 444
BpmI CTGGAG 1 cut(s) 1473
Bpu14I TTCGAA 1 cut(s) 892
BpuEI CTTGAG 1 cut(s) 1265
Bsa29I ATCGAT 1 cut(s) 979
BsaBI GATNNNNATC 1 cut(s) 1290
BsaJI CCNNGG 5 cut(s) 313, 486, 578, 666, 814
BsaWI WCCGGW 1 cut(s) 396
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 487, 672, 724, 1112
Bse118I RCCGGY 1 cut(s) 916
Bse1I ACTGG 3 cut(s) 663, 1074, 1456
Bse8I GATNNNNATC 1 cut(s) 1290
BseAI TCCGGA 1 cut(s) 396
BseCI ATCGAT 1 cut(s) 979
BseDI CCNNGG 5 cut(s) 313, 486, 578, 666, 814
BseGI GGATG 2 cut(s) 1183, 1483
BseJI GATNNNNATC 1 cut(s) 1290
BseLI CCNNNNNNNGG 5 cut(s) 185, 487, 672, 724, 1112
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 3 cut(s) 663, 1074, 1456
BseRI GAGGAG 5 cut(s) 61, 1001, 1191, 1227, 1230
BseXI GCAGC 5 cut(s) 90, 403, 460, 715, 1286
BsgI GTGCAG 1 cut(s) 353
BshFI GGCC 2 cut(s) 68, 761
BshVI ATCGAT 1 cut(s) 979
BsiSI CCGG 2 cut(s) 397, 917
BslFI GGGAC 3 cut(s) 100, 605, 1062
BslI CCNNNNNNNGG 5 cut(s) 185, 487, 672, 724, 1112
BsmFI GGGAC 3 cut(s) 100, 605, 1062
BsnI GGCC 2 cut(s) 68, 761
Bsp119I TTCGAA 1 cut(s) 892
Bsp1286I GDGCHC 2 cut(s) 179, 665
Bsp13I TCCGGA 1 cut(s) 396
Bsp143I GATC 7 cut(s) 195, 275, 622, 763, 976, 1255, 1307
BspACI CCGC 2 cut(s) 481, 904
BspANI GGCC 2 cut(s) 68, 761
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 979
BspEI TCCGGA 1 cut(s) 396
BspHI TCATGA 1 cut(s) 1437
BspLI GGNNCC 4 cut(s) 237, 664, 716, 1464
BspPI GGATC 3 cut(s) 203, 630, 1315
BspQI GCTCTTC 2 cut(s) 567, 1500
BspT104I TTCGAA 1 cut(s) 892
BsrBI CCGCTC 1 cut(s) 904
BsrFI RCCGGY 1 cut(s) 916
BsrI ACTGG 3 cut(s) 663, 1074, 1456
BssAI RCCGGY 1 cut(s) 916
BssECI CCNNGG 5 cut(s) 313, 486, 578, 666, 814
BssMI GATC 7 cut(s) 195, 275, 622, 763, 976, 1255, 1307
BssNAI GTATAC 1 cut(s) 354
BssT1I CCWWGG 3 cut(s) 578, 666, 814
Bst1107I GTATAC 1 cut(s) 354
Bst4CI ACNGT 3 cut(s) 91, 1253, 1418
Bst6I CTCTTC 7 cut(s) 284, 567, 688, 843, 950, 1377, 1500
BstBI TTCGAA 1 cut(s) 892
BstDEI CTNAG 3 cut(s) 228, 299, 800
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 2 cut(s) 1183, 1483
BstKTI GATC 7 cut(s) 198, 278, 625, 766, 979, 1258, 1310
BstMBI GATC 7 cut(s) 195, 275, 622, 763, 976, 1255, 1307
BstMWI GCNNNNNNNGC 2 cut(s) 103, 244
BstNSI RCATGY 1 cut(s) 332
BstV1I GCAGC 5 cut(s) 90, 403, 460, 715, 1286
BstV2I GAAGAC 2 cut(s) 69, 444
BstX2I RGATCY 2 cut(s) 622, 1307
BstYI RGATCY 2 cut(s) 622, 1307
BstZ17I GTATAC 1 cut(s) 354
Bsu15I ATCGAT 1 cut(s) 979
BsuI GTATCC 1 cut(s) 1441
BsuRI GGCC 2 cut(s) 68, 761
BsuTUI ATCGAT 1 cut(s) 979
BtgI CCRYGG 1 cut(s) 486
BtrI CACGTC 1 cut(s) 42
BtsCI GGATG 2 cut(s) 1183, 1483
BtsI GCAGTG 3 cut(s) 220, 341, 1082
BtsIMutI CAGTG 6 cut(s) 161, 220, 341, 609, 1082, 1423
CciI TCATGA 1 cut(s) 1437
Cfr10I RCCGGY 1 cut(s) 916
Cfr13I GGNCC 1 cut(s) 1463
ClaI ATCGAT 1 cut(s) 979
Csp6I GTAC 5 cut(s) 508, 631, 708, 853, 907
CviQI GTAC 5 cut(s) 508, 631, 708, 853, 907
DdeI CTNAG 3 cut(s) 228, 299, 800
DpnI GATC 7 cut(s) 197, 277, 624, 765, 978, 1257, 1309
DpnII GATC 7 cut(s) 195, 275, 622, 763, 976, 1255, 1307
DraIII CACNNNGTG 1 cut(s) 422
Eam1104I CTCTTC 7 cut(s) 284, 567, 688, 843, 950, 1377, 1500
EarI CTCTTC 7 cut(s) 284, 567, 688, 843, 950, 1377, 1500
EciI GGCGGA 1 cut(s) 470
Eco130I CCWWGG 3 cut(s) 578, 666, 814
Eco24I GRGCYC 2 cut(s) 179, 665
Eco32I GATATC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 1463
Eco57I CTGAAG 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 1463
EcoRI GAATTC 1 cut(s) 939
EcoRV GATATC 1 cut(s) 305
EcoT14I CCWWGG 3 cut(s) 578, 666, 814
EcoT38I GRGCYC 2 cut(s) 179, 665
ErhI CCWWGG 3 cut(s) 578, 666, 814
FaqI GGGAC 3 cut(s) 100, 605, 1062
FbaI TGATCA 1 cut(s) 1255
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 5 cut(s) 104, 392, 449, 729, 1275
FokI GGATG 2 cut(s) 1190, 1490
FriOI GRGCYC 2 cut(s) 179, 665
Fsp4HI GCNGC 5 cut(s) 104, 392, 449, 729, 1275
FspBI CTAG 3 cut(s) 357, 999, 1025
GluI GCNGC 5 cut(s) 104, 392, 449, 729, 1275
GsuI CTGGAG 1 cut(s) 1473
HaeIII GGCC 2 cut(s) 68, 761
HapII CCGG 2 cut(s) 397, 917
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 2 cut(s) 245, 296
HinfI GANTC 3 cut(s) 226, 491, 1037
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 2 cut(s) 397, 917
HphI GGTGA 3 cut(s) 298, 447, 1127
Hpy166II GTNNAC 4 cut(s) 354, 811, 855, 1414
Hpy188I TCNGA 3 cut(s) 231, 622, 1042
Hpy188III TCNNGA 9 cut(s) 397, 569, 767, 936, 1017, 1175, 1244, 1438, 1486
Hpy8I GTNNAC 4 cut(s) 354, 811, 855, 1414
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 5 cut(s) 168, 226, 462, 746, 1242
HpyCH4III ACNGT 3 cut(s) 91, 1253, 1418
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 8 cut(s) 334, 346, 383, 391, 448, 826, 1033, 1331
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 244
HpyF3I CTNAG 3 cut(s) 228, 299, 800
HpySE526I ACGT 1 cut(s) 41
Kpn2I TCCGGA 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 1255
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 7 cut(s) 195, 275, 622, 763, 976, 1255, 1307
LguI GCTCTTC 2 cut(s) 567, 1500
LmnI GCTCC 4 cut(s) 235, 399, 668, 1454
Lsp1109I GCAGC 5 cut(s) 90, 403, 460, 715, 1286
LweI GCATC 2 cut(s) 84, 835
MaeI CTAG 3 cut(s) 357, 999, 1025
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 254
MalI GATC 7 cut(s) 197, 277, 624, 765, 978, 1257, 1309
MbiI CCGCTC 1 cut(s) 904
MboI GATC 7 cut(s) 195, 275, 622, 763, 976, 1255, 1307
MfeI CAATTG 3 cut(s) 207, 279, 497
MflI RGATCY 2 cut(s) 622, 1307
MhlI GDGCHC 2 cut(s) 179, 665
MlyI GAGTC 3 cut(s) 220, 485, 1031
MmeI TCCRAC 1 cut(s) 203
MroI TCCGGA 1 cut(s) 396
MseI TTAA 3 cut(s) 609, 810, 1050
MslI CAYNNNNRTG 1 cut(s) 1419
MspI CCGG 2 cut(s) 397, 917
MunI CAATTG 3 cut(s) 207, 279, 497
MwoI GCNNNNNNNGC 2 cut(s) 103, 244
NdeII GATC 7 cut(s) 195, 275, 622, 763, 976, 1255, 1307
NlaIV GGNNCC 4 cut(s) 237, 664, 716, 1464
NmuCI GTSAC 1 cut(s) 254
NspI RCATGY 1 cut(s) 332
NspV TTCGAA 1 cut(s) 892
PagI TCATGA 1 cut(s) 1437
PciI ACATGT 1 cut(s) 328
PciSI GCTCTTC 2 cut(s) 567, 1500
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PkrI GCNGC 5 cut(s) 105, 393, 450, 730, 1276
PleI GAGTC 3 cut(s) 220, 485, 1031
PpsI GAGTC 3 cut(s) 220, 485, 1031
PpuMI RGGWCCY 1 cut(s) 1463
PscI ACATGT 1 cut(s) 328
Psp5II RGGWCCY 1 cut(s) 1463
PspN4I GGNNCC 4 cut(s) 237, 664, 716, 1464
PspPI GGNCC 1 cut(s) 1463
PspPPI RGGWCCY 1 cut(s) 1463
PsuI RGATCY 2 cut(s) 622, 1307
RsaI GTAC 5 cut(s) 509, 632, 709, 854, 908
RsaNI GTAC 5 cut(s) 508, 631, 708, 853, 907
RseI CAYNNNNRTG 1 cut(s) 1419
SapI GCTCTTC 2 cut(s) 567, 1500
SaqAI TTAA 3 cut(s) 609, 810, 1050
SatI GCNGC 5 cut(s) 104, 392, 449, 729, 1275
Sau3AI GATC 7 cut(s) 195, 275, 622, 763, 976, 1255, 1307
Sau96I GGNCC 1 cut(s) 1463
SchI GAGTC 3 cut(s) 220, 485, 1031
SduI GDGCHC 2 cut(s) 179, 665
SfaNI GCATC 2 cut(s) 84, 835
SfuI TTCGAA 1 cut(s) 892
SinI GGWCC 1 cut(s) 1463
SmiMI CAYNNNNRTG 1 cut(s) 1419
SmlI CTYRAG 1 cut(s) 1244
SmoI CTYRAG 1 cut(s) 1244
SpeI ACTAGT 2 cut(s) 356, 1024
SsiI CCGC 2 cut(s) 481, 904
SspMI CTAG 3 cut(s) 357, 999, 1025
StyI CCWWGG 3 cut(s) 578, 666, 814
TaaI ACNGT 3 cut(s) 91, 1253, 1418
TaiI ACGT 1 cut(s) 44
TaqI TCGA 5 cut(s) 5, 59, 691, 892, 979
TatI WGTACW 2 cut(s) 630, 852
Tru1I TTAA 3 cut(s) 609, 810, 1050
Tru9I TTAA 3 cut(s) 609, 810, 1050
TscAI CASTG 6 cut(s) 168, 220, 341, 609, 1089, 1423
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 5 cut(s) 103, 391, 448, 728, 1274
Tsp45I GTSAC 1 cut(s) 254
TspGWI ACGGA 2 cut(s) 503, 1226
TspRI CASTG 6 cut(s) 168, 220, 341, 609, 1089, 1423
VpaK11BI GGWCC 1 cut(s) 1463
XapI RAATTY 3 cut(s) 55, 585, 939
XceI RCATGY 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 353
XspI CTAG 3 cut(s) 357, 999, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.