Rroxscaffold_4G00304380
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012646
Physical Location & Seq
Reverse (-)
25064676 .. 25066390
1715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304380.1

Sequence Viewer

Length: 1653 bp
ATGGTGGCCTCCTCGTCTTCTTCTGGGTCTGTGATGCCAACGCTGCCGTTAGGTATGAAGATGTCATTCATCCCCACCGATTCACTGCTCATCAGGTTTATTCTCCACGATTCTGCCCCGAATTTTATTATTACTGATTGCGATCTATTTGGCCAGGAAGAGCCCTGGGAAATCTGGGACAGATATCTAGGGTTTCTAAACCAATACAACCCAGATGAACTTTACTTCTTCATGCCTTCGTTAAAGAAACTGAGCCCTAATGGTGGTTCAAGGATCAACCGAACAATTGGCGGTGGTAGTTGGACTCAGAAGGAGCCAATCAAGTTTGTGTGCGACGATGAGGACGAAGGCAGATCAATTGGGGTGAAGAGAAAGCTTAGATATCACAACCAAGGGTCAGATCAACATGTGCAGTGGTGTTTGGATGAGTATACTAGTGTTGAGGTTGCTAATTCATGTGCAATTTTTCGGCTCCGGAAAAACGAGCGACAATACAAATCACTATGTGGTATTTGTGTGGAAGACTTCATTCAATTAGGTATGAAGATTTTATTCCGCCCCACGGACTCACAATTGATTAGGTACTTTCTTGGTTTTGATATTGCCCCAGATTTTATTGTTTATGATTGTGATTTGTTTGGTCAGGAAGAGCCTTGGGAAATTTGGGACAGGTATTCCGTGGTTTTTAACAACTCAGACCTTTTGTACTTTTTCACATCGTCTATGAAGAAACTGGGCTCCAAGGGGAGGGTTTGTAATTTTGGTCGAAGAGTCGGGTCTGGTACATGGAACCAAAAGGAGGCTGCCAAATTGGTGTATGATGATGAAGGCGAGGCCGATCAAGAAAGAAAGCCAATTGGGAGAAAGAGGAAACTTAGATATGTTAACCAAGGCTCTGTGCATCATGGGGAGTGGTATTTGGAAGAGTACACAAGCCTTGTTGATGAGGGAATTCCAAGTGAAAAGAAGAGGAAACACAGAGAAATTGATCGATTGGAGGAGAAGAAGCCTAGAAAGAAAAAAGCGACAAGACAACTAGTAATGCAGGACTCGGATTTACTTAATAGGCAAAGCCAAACTACTACCGGTCCCATAGTGCCTATTCAACAACGAGAACCTGGCAATGGTGATGATAATTTCGTGTTCTTCAAGGGTATTGATATTGGAACATGGGAGTTTCCTTCTTTGGATGATTTTGTCATGGCTTGCCCTCCTCCACTTACGGAATGTGATCAAGAAGCATTAGCAACCCTTGAGTTTCCTTCTCTGGATGAATTTGCTTGTTCTCCTCCACTTACAGATTGCGATCAAGAAGCATTAGCAACCCTTGAGTTTCCTTCTCCAGATGACTTGGCTTCTCCTTCTCCTCCTCTTGAGGACTGTGACCAACAAGTAATAGCAGCAGTCAATATTGATGATAATCATGATCTCACGGGATCCTGTAATGATGATGATATTGCAACATGGAGTTTTCCTATTGTGGACTATTCATTGACTTCTCTTCTTCAAGGAAAATGTGATGAACAAGCATTAGCATTAGTCAACAGCGATGATGATCTACATTTCGTGAAAGAGGATACTGGAGCATGGTGTCCAGCTTTTGTGCATGATTTCTTGATGGCTCTTCTTGTGGACTGTGATGAACAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

550

Amino Acids

62.59

Weight (kDa)

4.61

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 21 - 145 9.5e-15 No apical meristem (NAM) protein
NAM PF02365 184 - 311 4.7e-13 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 431
AccIII TCCGGA 1 cut(s) 474
AciI CCGC 2 cut(s) 291, 556
AclWI GGATC 3 cut(s) 281, 1431, 1444
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 4 cut(s) 121, 660, 951, 1274
AdeI CACNNNGTG 1 cut(s) 506
AfaI GTAC 4 cut(s) 584, 707, 784, 929
AfiI CCNNNNNNNGG 5 cut(s) 263, 562, 747, 799, 1124
AflIII ACRYGT 1 cut(s) 406
AgeI ACCGGT 1 cut(s) 1085
AgsI TTSAA 5 cut(s) 270, 533, 1106, 1150, 1508
AhlI ACTAGT 2 cut(s) 434, 1036
AjnI CCWGG 3 cut(s) 153, 164, 1117
AluBI AGCT 2 cut(s) 376, 1598
AluI AGCT 2 cut(s) 376, 1598
AlwI GGATC 3 cut(s) 281, 1431, 1444
Aor13HI TCCGGA 1 cut(s) 474
AoxI GGCC 3 cut(s) 6, 151, 834
ApeKI GCWGC 3 cut(s) 43, 803, 1400
ApoI RAATTY 4 cut(s) 121, 660, 951, 1274
AsiGI ACCGGT 1 cut(s) 1085
AspS9I GGNCC 1 cut(s) 1088
AsuHPI GGTGA 2 cut(s) 376, 1139
AvaII GGWCC 1 cut(s) 1088
BalI TGGCCA 1 cut(s) 153
BamHI GGATCC 1 cut(s) 1436
BanII GRGCYC 3 cut(s) 165, 257, 740
BbsI GAAGAC 2 cut(s) 9, 528
BbvI GCAGC 3 cut(s) 30, 790, 1412
BccI CCATC 1 cut(s) 1612
BceAI ACGGC 1 cut(s) 31
BciT130I CCWGG 3 cut(s) 155, 166, 1119
BciVI GTATCC 1 cut(s) 1570
BclI TGATCA 1 cut(s) 1231
BcuI ACTAGT 2 cut(s) 434, 1036
BfaI CTAG 4 cut(s) 188, 435, 1011, 1037
BfuI GTATCC 1 cut(s) 1570
BisI GCNGC 3 cut(s) 44, 804, 1401
BlsI GCNGC 3 cut(s) 45, 805, 1402
Bme1390I CCNGG 3 cut(s) 155, 166, 1119
Bme18I GGWCC 1 cut(s) 1088
BmgT120I GGNCC 1 cut(s) 1088
BmiI GGNNCC 6 cut(s) 315, 473, 739, 791, 1090, 1438
BmrFI CCNGG 3 cut(s) 155, 166, 1119
BmrI ACTGGG 1 cut(s) 743
BmsI GCATC 2 cut(s) 24, 910
BmuI ACTGGG 1 cut(s) 743
BpiI GAAGAC 2 cut(s) 9, 528
BpmI CTGGAG 2 cut(s) 1326, 1602
BpuEI CTTGAG 3 cut(s) 1274, 1349, 1394
Bsa29I ATCGAT 1 cut(s) 991
BsaBI GATNNNNATC 4 cut(s) 141, 1305, 1419, 1554
BsaJI CCNNGG 8 cut(s) 164, 165, 391, 561, 653, 678, 741, 889
BsaWI WCCGGW 2 cut(s) 474, 1085
BsaXI ACNNNNNCTCC 2 cut(s) 739, 769
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 562, 747, 799, 1124
Bse118I RCCGGY 1 cut(s) 1085
Bse1I ACTGG 2 cut(s) 738, 1585
Bse3DI GCAATG 1 cut(s) 1129
Bse8I GATNNNNATC 4 cut(s) 141, 1305, 1419, 1554
BseAI TCCGGA 1 cut(s) 474
BseBI CCWGG 3 cut(s) 155, 166, 1119
BseCI ATCGAT 1 cut(s) 991
BseDI CCNNGG 8 cut(s) 164, 165, 391, 561, 653, 678, 741, 889
BseGI GGATG 4 cut(s) 69, 430, 1195, 1276
BseJI GATNNNNATC 4 cut(s) 141, 1305, 1419, 1554
BseLI CCNNNNNNNGG 5 cut(s) 263, 562, 747, 799, 1124
BseMI GCAATG 1 cut(s) 1129
BseMII CTCAG 3 cut(s) 242, 320, 708
BseNI ACTGG 2 cut(s) 738, 1585
BseRI GAGGAG 5 cut(s) 1013, 1203, 1278, 1356, 1359
BseXI GCAGC 3 cut(s) 30, 790, 1412
BsgI GTGCAG 1 cut(s) 431
BshFI GGCC 3 cut(s) 8, 153, 836
BshTI ACCGGT 1 cut(s) 1085
BshVI ATCGAT 1 cut(s) 991
BsiSI CCGG 2 cut(s) 475, 1086
BslFI GGGAC 3 cut(s) 191, 680, 1074
BslI CCNNNNNNNGG 5 cut(s) 263, 562, 747, 799, 1124
BsmFI GGGAC 3 cut(s) 191, 680, 1074
BsnI GGCC 3 cut(s) 8, 153, 836
Bsp1286I GDGCHC 3 cut(s) 165, 257, 740
Bsp13I TCCGGA 1 cut(s) 474
BspACI CCGC 2 cut(s) 291, 556
BspANI GGCC 3 cut(s) 8, 153, 836
BspCNI CTCAG 3 cut(s) 243, 319, 707
BspDI ATCGAT 1 cut(s) 991
BspEI TCCGGA 1 cut(s) 474
BspHI TCATGA 1 cut(s) 1423
BspLI GGNNCC 6 cut(s) 315, 473, 739, 791, 1090, 1438
BspPI GGATC 3 cut(s) 281, 1431, 1444
BspQI GCTCTTC 3 cut(s) 153, 642, 1629
BsrDI GCAATG 1 cut(s) 1129
BsrFI RCCGGY 1 cut(s) 1085
BsrI ACTGG 2 cut(s) 738, 1585
BssAI RCCGGY 1 cut(s) 1085
BssECI CCNNGG 8 cut(s) 164, 165, 391, 561, 653, 678, 741, 889
BssNAI GTATAC 1 cut(s) 432
BssT1I CCWWGG 4 cut(s) 391, 653, 741, 889
Bst1107I GTATAC 1 cut(s) 432
Bst2UI CCWGG 3 cut(s) 155, 166, 1119
Bst4CI ACNGT 2 cut(s) 1382, 1637
Bst6I CTCTTC 8 cut(s) 153, 362, 642, 763, 918, 962, 1506, 1629
BstC8I GCNNGC 1 cut(s) 1207
BstDEI CTNAG 5 cut(s) 251, 306, 377, 694, 875
BstDSI CCRYGG 2 cut(s) 561, 678
BstF5I GGATG 4 cut(s) 69, 430, 1195, 1276
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstNI CCWGG 3 cut(s) 155, 166, 1119
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 3 cut(s) 153, 164, 1117
BstV1I GCAGC 3 cut(s) 30, 790, 1412
BstV2I GAAGAC 2 cut(s) 9, 528
BstX2I RGATCY 1 cut(s) 1436
BstYI RGATCY 1 cut(s) 1436
BstZ17I GTATAC 1 cut(s) 432
Bsu15I ATCGAT 1 cut(s) 991
BsuI GTATCC 1 cut(s) 1570
BsuRI GGCC 3 cut(s) 8, 153, 836
BsuTUI ATCGAT 1 cut(s) 991
BtgI CCRYGG 2 cut(s) 561, 678
BtgZI GCGATG 1 cut(s) 1563
BtsCI GGATG 4 cut(s) 69, 430, 1195, 1276
BtsI GCAGTG 2 cut(s) 83, 419
BtsIMutI CAGTG 2 cut(s) 83, 419
Cac8I GCNNGC 1 cut(s) 1207
CciI TCATGA 1 cut(s) 1423
Cfr10I RCCGGY 1 cut(s) 1085
Cfr13I GGNCC 1 cut(s) 1088
ClaI ATCGAT 1 cut(s) 991
Csp6I GTAC 4 cut(s) 583, 706, 783, 928
CspAI ACCGGT 1 cut(s) 1085
CviQI GTAC 4 cut(s) 583, 706, 783, 928
DdeI CTNAG 5 cut(s) 251, 306, 377, 694, 875
DraIII CACNNNGTG 1 cut(s) 506
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 8 cut(s) 153, 362, 642, 763, 918, 962, 1506, 1629
EarI CTCTTC 8 cut(s) 153, 362, 642, 763, 918, 962, 1506, 1629
EciI GGCGGA 1 cut(s) 545
Eco130I CCWWGG 4 cut(s) 391, 653, 741, 889
Eco24I GRGCYC 3 cut(s) 165, 257, 740
Eco32I GATATC 2 cut(s) 185, 383
Eco47I GGWCC 1 cut(s) 1088
EcoRI GAATTC 1 cut(s) 951
EcoRII CCWGG 3 cut(s) 153, 164, 1117
EcoRV GATATC 2 cut(s) 185, 383
EcoT14I CCWWGG 4 cut(s) 391, 653, 741, 889
EcoT38I GRGCYC 3 cut(s) 165, 257, 740
ErhI CCWWGG 4 cut(s) 391, 653, 741, 889
FaqI GGGAC 3 cut(s) 191, 680, 1074
FbaI TGATCA 1 cut(s) 1231
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 3 cut(s) 44, 804, 1401
FokI GGATG 4 cut(s) 56, 437, 1202, 1283
FriOI GRGCYC 3 cut(s) 165, 257, 740
Fsp4HI GCNGC 3 cut(s) 44, 804, 1401
FspBI CTAG 4 cut(s) 188, 435, 1011, 1037
GluI GCNGC 3 cut(s) 44, 804, 1401
GsuI CTGGAG 2 cut(s) 1326, 1602
HaeIII GGCC 3 cut(s) 8, 153, 836
HapII CCGG 2 cut(s) 475, 1086
HincII GTYRAC 2 cut(s) 886, 1543
HindII GTYRAC 2 cut(s) 886, 1543
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 6 cut(s) 80, 110, 304, 566, 771, 1049
HpaI GTTAAC 1 cut(s) 886
HpaII CCGG 2 cut(s) 475, 1086
HphI GGTGA 2 cut(s) 376, 1139
Hpy166II GTNNAC 6 cut(s) 432, 886, 930, 1483, 1543, 1633
Hpy188I TCNGA 4 cut(s) 309, 400, 697, 1054
Hpy8I GTNNAC 6 cut(s) 432, 886, 930, 1483, 1543, 1633
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 8 cut(s) 246, 304, 341, 821, 1191, 1272, 1347, 1371
HpyCH4III ACNGT 2 cut(s) 1382, 1637
HpyCH4V TGCA 6 cut(s) 412, 461, 901, 1045, 1460, 1606
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpyF3I CTNAG 5 cut(s) 251, 306, 377, 694, 875
Kpn2I TCCGGA 1 cut(s) 474
Ksp22I TGATCA 1 cut(s) 1231
KspAI GTTAAC 1 cut(s) 886
LguI GCTCTTC 3 cut(s) 153, 642, 1629
LmnI GCTCC 4 cut(s) 313, 477, 743, 1583
Lsp1109I GCAGC 3 cut(s) 30, 790, 1412
LweI GCATC 2 cut(s) 24, 910
MaeI CTAG 4 cut(s) 188, 435, 1011, 1037
MaeIII GTNAC 1 cut(s) 1382
MfeI CAATTG 4 cut(s) 285, 357, 572, 855
MflI RGATCY 1 cut(s) 1436
MhlI GDGCHC 3 cut(s) 165, 257, 740
MlsI TGGCCA 1 cut(s) 153
MluNI TGGCCA 1 cut(s) 153
MlyI GAGTC 4 cut(s) 298, 560, 780, 1043
MmeI TCCRAC 1 cut(s) 281
Mox20I TGGCCA 1 cut(s) 153
MroI TCCGGA 1 cut(s) 474
MscI TGGCCA 1 cut(s) 153
MseI TTAA 4 cut(s) 242, 687, 885, 1062
Msp20I TGGCCA 1 cut(s) 153
MspI CCGG 2 cut(s) 475, 1086
MspR9I CCNGG 3 cut(s) 155, 166, 1119
MunI CAATTG 4 cut(s) 285, 357, 572, 855
MvaI CCWGG 3 cut(s) 155, 166, 1119
MwoI GCNNNNNNNGC 1 cut(s) 43
NlaIV GGNNCC 6 cut(s) 315, 473, 739, 791, 1090, 1438
NmuCI GTSAC 1 cut(s) 1382
NspI RCATGY 1 cut(s) 410
PagI TCATGA 1 cut(s) 1423
PasI CCCWGGG 1 cut(s) 165
PciI ACATGT 1 cut(s) 406
PciSI GCTCTTC 3 cut(s) 153, 642, 1629
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 2 cut(s) 80, 110
PinAI ACCGGT 1 cut(s) 1085
PkrI GCNGC 3 cut(s) 45, 805, 1402
PleI GAGTC 4 cut(s) 298, 560, 779, 1043
PpsI GAGTC 4 cut(s) 298, 560, 779, 1043
PscI ACATGT 1 cut(s) 406
Psp6I CCWGG 3 cut(s) 153, 164, 1117
PspGI CCWGG 3 cut(s) 153, 164, 1117
PspN4I GGNNCC 6 cut(s) 315, 473, 739, 791, 1090, 1438
PspPI GGNCC 1 cut(s) 1088
PsuI RGATCY 1 cut(s) 1436
RsaI GTAC 4 cut(s) 584, 707, 784, 929
RsaNI GTAC 4 cut(s) 583, 706, 783, 928
SapI GCTCTTC 3 cut(s) 153, 642, 1629
SaqAI TTAA 4 cut(s) 242, 687, 885, 1062
SatI GCNGC 3 cut(s) 44, 804, 1401
Sau96I GGNCC 1 cut(s) 1088
SchI GAGTC 4 cut(s) 298, 560, 780, 1043
ScrFI CCNGG 3 cut(s) 155, 166, 1119
SduI GDGCHC 3 cut(s) 165, 257, 740
SfaNI GCATC 2 cut(s) 24, 910
SinI GGWCC 1 cut(s) 1088
SmlI CTYRAG 3 cut(s) 1253, 1328, 1373
SmoI CTYRAG 3 cut(s) 1253, 1328, 1373
SpeI ACTAGT 2 cut(s) 434, 1036
SsiI CCGC 2 cut(s) 291, 556
SspI AATATT 1 cut(s) 1411
SspMI CTAG 4 cut(s) 188, 435, 1011, 1037
StyD4I CCNGG 3 cut(s) 153, 164, 1117
StyI CCWWGG 4 cut(s) 391, 653, 741, 889
TaaI ACNGT 2 cut(s) 1382, 1637
TaqI TCGA 2 cut(s) 766, 991
TatI WGTACW 2 cut(s) 705, 927
TfiI GAWTC 2 cut(s) 80, 110
Tru1I TTAA 4 cut(s) 242, 687, 885, 1062
Tru9I TTAA 4 cut(s) 242, 687, 885, 1062
TscAI CASTG 2 cut(s) 90, 419
TseFI GTSAC 1 cut(s) 1382
TseI GCWGC 3 cut(s) 43, 803, 1400
Tsp45I GTSAC 1 cut(s) 1382
TspGWI ACGGA 3 cut(s) 578, 667, 1238
TspRI CASTG 2 cut(s) 90, 419
VpaK11BI GGWCC 1 cut(s) 1088
XapI RAATTY 4 cut(s) 121, 660, 951, 1274
XceI RCATGY 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 431
XspI CTAG 4 cut(s) 188, 435, 1011, 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.