RLG00000035838

Scarecrow-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013778
Physical Location & Seq
Reverse (-)
72490469 .. 72491093
625 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035838

Sequence Viewer

Length: 534 bp
ATGATTTTCATGATCGCGAATTCAGAAACCAATTTAGAGATTAAGAGGGAGAGACCTGTTGGTAATCTTAGGAAGGAACAACCAAGGCGTCAAGAGGAGGAGAGTGAGACTCCATTAGAGACCTCTGTTCTTCATATGAGAAGAAGCCCGAGCCCACCCACTCTCTCCTCCTCCTTCAACGGCGGCGGCAACGACAACACCACCACGCCGCTTCTGCAGGACACTTCGTCTGTGGGACCCGAGCCCGACACGGCGGCGGCGCGCAGCAGCAGCAAGGATGAGTGGACCTGCGAGCTTCAGTGGATTCCCAGCGGCGGTCTGGAGGCCGTGACCGGATCGGGCGGCGGGAGATGCGGCGGGCTTGGATTGGAGGACTGGGAGACCATGCTGTCCGAAACGGCGGCGTCTCCGGGTCAAGACCAGTCGCTGCTCCAGTGGATCTCAGGCGACGTGGACGACGCGGGTCCGGTTTCGACCACATCAGAAGCAGCGTTTCCGGTCCCAATTTGGGGTTCTCCAGCGGTTCTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

178

Amino Acids

18.83

Weight (kDa)

4.32

Isoelectric Point (pI)

70.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 388
Acc36I ACCTGC 1 cut(s) 296
AccII CGCG 3 cut(s) 17, 262, 461
AclWI GGATC 2 cut(s) 343, 446
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 281
AcyI GRCGYC 2 cut(s) 88, 404
AfiI CCNNNNNNNGG 3 cut(s) 314, 508, 509
AgsI TTSAA 1 cut(s) 178
AhdI GACNNNNNGTC 1 cut(s) 226
AjiI CACGTC 1 cut(s) 451
AjuI GAANNNNNNNTTGG 2 cut(s) 496, 528
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
Alw26I GTCTC 5 cut(s) 46, 101, 113, 374, 411
AlwI GGATC 2 cut(s) 343, 446
AlwNI CAGNNNCTG 1 cut(s) 427
Ama87I CYCGRG 2 cut(s) 148, 239
AoxI GGCC 1 cut(s) 324
ApeKI GCWGC 5 cut(s) 264, 267, 270, 427, 488
ApoI RAATTY 1 cut(s) 19
AspLEI GCGC 2 cut(s) 262, 264
AspS9I GGNCC 4 cut(s) 236, 285, 464, 499
AsuC2I CCSGG 1 cut(s) 411
AvaI CYCGRG 2 cut(s) 148, 239
AvaII GGWCC 4 cut(s) 236, 285, 464, 499
BanII GRGCYC 2 cut(s) 155, 246
BbvI GCAGC 5 cut(s) 276, 279, 282, 414, 500
BceAI ACGGC 4 cut(s) 196, 267, 311, 414
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 5 cut(s) 46, 101, 113, 374, 411
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 296
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 4 cut(s) 236, 285, 464, 499
BmeRI GACNNNNNGTC 1 cut(s) 226
BmeT110I CYCGRG 2 cut(s) 148, 239
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 4 cut(s) 236, 285, 464, 499
BmiI GGNNCC 4 cut(s) 237, 238, 465, 501
BmrFI CCNGG 1 cut(s) 411
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 1 cut(s) 341
BmuI ACTGGG 1 cut(s) 385
BoxI GACNNNNGTC 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 94, 126
BpmI CTGGAG 3 cut(s) 341, 416, 501
BpuMI CCSGG 1 cut(s) 411
BsaHI GRCGYC 2 cut(s) 88, 404
BsaI GGTCTC 3 cut(s) 46, 113, 374
BsaJI CCNNGG 1 cut(s) 83
BsaWI WCCGGW 3 cut(s) 332, 466, 496
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 508, 509
Bse1I ACTGG 3 cut(s) 380, 421, 433
BseDI CCNNGG 1 cut(s) 83
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 3 cut(s) 314, 508, 509
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 3 cut(s) 380, 421, 433
BsePI GCGCGC 1 cut(s) 260
BseRI GAGGAG 4 cut(s) 110, 113, 157, 160
BseXI GCAGC 5 cut(s) 276, 279, 282, 414, 500
BseYI CCCAGC 1 cut(s) 308
Bsh1236I CGCG 3 cut(s) 17, 262, 461
BshFI GGCC 1 cut(s) 326
BsiHKCI CYCGRG 2 cut(s) 148, 239
BsiSI CCGG 4 cut(s) 333, 410, 467, 497
BslFI GGGAC 2 cut(s) 249, 485
BslI CCNNNNNNNGG 3 cut(s) 314, 508, 509
BsmAI GTCTC 5 cut(s) 46, 101, 113, 374, 411
BsmBI CGTCTC 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 249, 485
BsnI GGCC 1 cut(s) 326
Bso31I GGTCTC 3 cut(s) 46, 113, 374
BsoBI CYCGRG 2 cut(s) 148, 239
Bsp1286I GDGCHC 2 cut(s) 155, 246
Bsp143I GATC 3 cut(s) 12, 335, 438
Bsp68I TCGCGA 1 cut(s) 17
BspANI GGCC 1 cut(s) 326
BspCNI CTCAG 1 cut(s) 455
BspFNI CGCG 3 cut(s) 17, 262, 461
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 4 cut(s) 237, 238, 465, 501
BspMAI CTGCAG 1 cut(s) 219
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 2 cut(s) 343, 446
BspTNI GGTCTC 3 cut(s) 46, 113, 374
BsrI ACTGG 3 cut(s) 380, 421, 433
BssECI CCNNGG 1 cut(s) 83
BssHII GCGCGC 1 cut(s) 260
BssMI GATC 3 cut(s) 12, 335, 438
BssNI GRCGYC 2 cut(s) 88, 404
BssT1I CCWWGG 1 cut(s) 83
BstACI GRCGYC 2 cut(s) 88, 404
BstC8I GCNNGC 3 cut(s) 262, 293, 359
BstDEI CTNAG 2 cut(s) 68, 442
BstF5I GGATG 1 cut(s) 283
BstFNI CGCG 3 cut(s) 17, 262, 461
BstHHI GCGC 2 cut(s) 262, 264
BstKTI GATC 3 cut(s) 15, 338, 441
BstMAI GTCTC 5 cut(s) 46, 101, 113, 374, 411
BstMBI GATC 3 cut(s) 12, 335, 438
BstMWI GCNNNNNNNGC 3 cut(s) 214, 270, 351
BstPAI GACNNNNGTC 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 409
BstSFI CTRYAG 1 cut(s) 215
BstUI CGCG 3 cut(s) 17, 262, 461
BstV1I GCAGC 5 cut(s) 276, 279, 282, 414, 500
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuRI GGCC 1 cut(s) 326
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 305, 440
BtuMI TCGCGA 1 cut(s) 17
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 3 cut(s) 262, 293, 359
CaiI CAGNNNCTG 1 cut(s) 427
CciI TCATGA 1 cut(s) 9
CfoI GCGC 2 cut(s) 262, 264
Cfr13I GGNCC 4 cut(s) 236, 285, 464, 499
CseI GACGC 3 cut(s) 77, 393, 467
CviAII CATG 2 cut(s) 10, 385
CviJI RGCY 6 cut(s) 147, 153, 244, 295, 326, 361
CviKI_1 RGCY 6 cut(s) 147, 153, 244, 295, 326, 361
DdeI CTNAG 2 cut(s) 68, 442
DpnI GATC 3 cut(s) 14, 337, 440
DpnII GATC 3 cut(s) 12, 335, 438
DrdI GACNNNNNNGTC 1 cut(s) 388
DriI GACNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 388
Eam1105I GACNNNNNGTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 83
Eco24I GRGCYC 2 cut(s) 155, 246
Eco31I GGTCTC 3 cut(s) 46, 113, 374
Eco47I GGWCC 4 cut(s) 236, 285, 464, 499
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 2 cut(s) 148, 239
EcoO109I RGGNCCY 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 83
EcoT38I GRGCYC 2 cut(s) 155, 246
ErhI CCWWGG 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 411
FaeI CATG 2 cut(s) 13, 388
FaiI YATR 4 cut(s) 11, 135, 137, 386
FaqI GGGAC 2 cut(s) 249, 485
FatI CATG 2 cut(s) 9, 384
FauI CCCGC 3 cut(s) 338, 350, 454
FauNDI CATATG 1 cut(s) 135
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 2 cut(s) 155, 246
FspBI CTAG 1 cut(s) 527
GlaI GCGC 2 cut(s) 261, 263
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 3 cut(s) 341, 416, 501
HaeIII GGCC 1 cut(s) 326
HapII CCGG 4 cut(s) 333, 410, 467, 497
HgaI GACGC 3 cut(s) 77, 393, 467
HhaI GCGC 2 cut(s) 262, 264
Hin1I GRCGYC 2 cut(s) 88, 404
Hin1II CATG 2 cut(s) 13, 388
Hin6I GCGC 2 cut(s) 260, 262
HinP1I GCGC 2 cut(s) 260, 262
HinfI GANTC 2 cut(s) 109, 304
HpaII CCGG 4 cut(s) 333, 410, 467, 497
Hpy166II GTNNAC 2 cut(s) 285, 454
Hpy188I TCNGA 3 cut(s) 25, 394, 484
Hpy188III TCNNGA 5 cut(s) 10, 16, 92, 320, 416
Hpy8I GTNNAC 2 cut(s) 285, 454
Hpy99I CGWCG 2 cut(s) 452, 461
HpyAV CCTTC 2 cut(s) 67, 184
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 1 cut(s) 217
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 270, 351
HpyF3I CTNAG 2 cut(s) 68, 442
HpySE526I ACGT 1 cut(s) 450
Hsp92I GRCGYC 2 cut(s) 88, 404
Hsp92II CATG 2 cut(s) 13, 388
HspAI GCGC 2 cut(s) 260, 262
KflI GGGWCCC 1 cut(s) 236
Kzo9I GATC 3 cut(s) 12, 335, 438
LmnI GCTCC 1 cut(s) 435
Lsp1109I GCAGC 5 cut(s) 276, 279, 282, 414, 500
LweI GCATC 1 cut(s) 341
MaeI CTAG 1 cut(s) 527
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 1 cut(s) 328
MalI GATC 3 cut(s) 14, 337, 440
MboI GATC 3 cut(s) 12, 335, 438
MboII GAAGA 2 cut(s) 122, 153
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 2 cut(s) 155, 246
MluCI AATT 3 cut(s) 19, 31, 504
MlyI GAGTC 1 cut(s) 103
MnlI CCTC 8 cut(s) 39, 88, 91, 133, 178, 181, 316, 364
MseI TTAA 1 cut(s) 42
MspA1I CMGCKG 2 cut(s) 312, 521
MspI CCGG 4 cut(s) 333, 410, 467, 497
MspR9I CCNGG 1 cut(s) 411
MvnI CGCG 3 cut(s) 17, 262, 461
MwoI GCNNNNNNNGC 3 cut(s) 214, 270, 351
NciI CCSGG 1 cut(s) 411
NdeI CATATG 1 cut(s) 135
NdeII GATC 3 cut(s) 12, 335, 438
NlaIII CATG 2 cut(s) 13, 388
NlaIV GGNNCC 4 cut(s) 237, 238, 465, 501
NmuCI GTSAC 1 cut(s) 328
NruI TCGCGA 1 cut(s) 17
PagI TCATGA 1 cut(s) 9
PauI GCGCGC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 304
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 236
PshAI GACNNNNGTC 1 cut(s) 462
Psp5II RGGWCCY 1 cut(s) 236
PspFI CCCAGC 1 cut(s) 308
PspN4I GGNNCC 4 cut(s) 237, 238, 465, 501
PspPI GGNCC 4 cut(s) 236, 285, 464, 499
PspPPI RGGWCCY 1 cut(s) 236
PstI CTGCAG 1 cut(s) 219
PstNI CAGNNNCTG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 438
PteI GCGCGC 1 cut(s) 260
RruI TCGCGA 1 cut(s) 17
SaqAI TTAA 1 cut(s) 42
Sau3AI GATC 3 cut(s) 12, 335, 438
Sau96I GGNCC 4 cut(s) 236, 285, 464, 499
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 2 cut(s) 155, 246
SetI ASST 5 cut(s) 58, 125, 290, 297, 453
SfaNI GCATC 1 cut(s) 341
SfcI CTRYAG 1 cut(s) 215
SinI GGWCC 4 cut(s) 236, 285, 464, 499
Sse9I AATT 3 cut(s) 19, 31, 504
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 1 cut(s) 409
StyI CCWWGG 1 cut(s) 83
TaiI ACGT 1 cut(s) 453
TaqI TCGA 1 cut(s) 473
TasI AATT 3 cut(s) 19, 31, 504
TauI GCSGC 9 cut(s) 186, 189, 211, 257, 260, 315, 345, 357, 404
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TscAI CASTG 2 cut(s) 305, 440
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 5 cut(s) 264, 267, 270, 427, 488
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 1 cut(s) 122
TspRI CASTG 2 cut(s) 305, 440
VpaK11BI GGWCC 4 cut(s) 236, 285, 464, 499
XapI RAATTY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 316
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.