Rroxscaffold_1G00017100

Scarecrow-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013778
Physical Location & Seq
Forward (+)
21292465 .. 21293259
795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00017100.1

Sequence Viewer

Length: 795 bp
ATGAGAAGAAGCCCGAGCCCACCCACTTCGGCATCCACTCTCTCCTCCTCCTTCAACAGCGGCGGCAACGGCAACACCACCACGCCGCTTCTGCAGGACACTTCGCAGGTGGGACCTGAGCCCGACACGACGGCAGCGCGTAGCAGTAGCAAGGATGAGTGGACCTGCGAGCTTCAGTCGATTCCCAGAGGCGGTCTGGAGGCAGTGACCGGATCAGGCAGCGAGAGATGCGGCTGGCTTGGGTTGGAGGACTGGGAGACCATGCTGTCCGAAACGGCGGCGTCTTCGGGTCAAGACCAGTCTCTGCTCCGGTGGATCTCGGGCGATGTGGACGACGCGTCGTTTGGGCTAAAGCAGCTGTTGCAGAGTGGGAACAACAGTAACTCTCTTGAGTTTGACGGCAATGCGGGTCTGGGAATGGTTGATCAGGGTTCCAATTTCGACCACATCGGAAGCAGCGTTTTCAGTCCCAATTTGGGGTTCTCCTGCGGTTCTAGTTTCAATGGGAAAATGGGTTTTGTGCCCAGCTCTTCTGGGCTGCTGAATTACAAGGTTTCAAATGTTGGGTTGATGAGCAATAATGGTGGCAACAACAACAACTCCAATTTCTCCAATTCAGCACTTCCAGTTTCGCTGCCTCATGGAATGGTCTATCAGAATCACCAGCAGTTTGAGAACCCAGATGAAAAGCCTCAGATTCTGAACCCACAAGTGTTCATGAACCAACACCAGTCTCAGCTCCCTCATCAAAATCCAAACTTTTTCATGCCAGTGACTTCTGCTCAACAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

264

Amino Acids

28.13

Weight (kDa)

4.6

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 97
AasI GACNNNNNNGTC 1 cut(s) 265
Acc36I ACCTGC 2 cut(s) 97, 173
AccII CGCG 2 cut(s) 139, 338
AciI CCGC 8 cut(s) 60, 63, 86, 192, 231, 278, 407, 489
AclWI GGATC 2 cut(s) 220, 323
AcuI CTGAAG 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 281
AfiI CCNNNNNNNGG 3 cut(s) 191, 476, 477
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 3 cut(s) 55, 502, 558
AhdI GACNNNNNGTC 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AluBI AGCT 4 cut(s) 172, 358, 528, 739
AluI AGCT 4 cut(s) 172, 358, 528, 739
Alw26I GTCTC 3 cut(s) 251, 306, 738
AlwI GGATC 2 cut(s) 220, 323
AlwNI CAGNNNCTG 2 cut(s) 304, 700
Ama87I CYCGRG 2 cut(s) 13, 319
ApeKI GCWGC 6 cut(s) 134, 219, 355, 456, 538, 634
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 2 cut(s) 113, 162
AsuHPI GGTGA 1 cut(s) 653
AvaI CYCGRG 2 cut(s) 13, 319
AvaII GGWCC 2 cut(s) 113, 162
BaeGI GKGCMC 1 cut(s) 525
BanII GRGCYC 2 cut(s) 20, 123
BbsI GAAGAC 1 cut(s) 276
BbvI GCAGC 6 cut(s) 146, 231, 367, 468, 525, 621
BceAI ACGGC 4 cut(s) 85, 147, 291, 415
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 3 cut(s) 251, 306, 738
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 1 cut(s) 92
BfuAI ACCTGC 2 cut(s) 97, 173
Bme18I GGWCC 2 cut(s) 113, 162
BmeRI GACNNNNNGTC 1 cut(s) 337
BmeT110I CYCGRG 2 cut(s) 13, 319
BmgT120I GGNCC 2 cut(s) 113, 162
BmiI GGNNCC 2 cut(s) 114, 433
BmrI ACTGGG 1 cut(s) 262
BmsI GCATC 2 cut(s) 41, 218
BmuI ACTGGG 1 cut(s) 262
BpiI GAAGAC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 218
Bpu10I CCTNAGC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 410
BsaHI GRCGYC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 251
BsaWI WCCGGW 2 cut(s) 209, 309
BsaXI ACNNNNNCTCC 2 cut(s) 584, 614
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 476, 477
Bse1I ACTGG 5 cut(s) 257, 298, 626, 730, 770
Bse3DI GCAATG 1 cut(s) 409
BseGI GGATG 2 cut(s) 32, 160
BseLI CCNNNNNNNGG 3 cut(s) 191, 476, 477
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 3 cut(s) 108, 707, 749
BseNI ACTGG 5 cut(s) 257, 298, 626, 730, 770
BseRI GAGGAG 2 cut(s) 34, 37
BseSI GKGCMC 1 cut(s) 525
BseXI GCAGC 6 cut(s) 146, 231, 367, 468, 525, 621
BseYI CCCAGC 1 cut(s) 524
Bsh1236I CGCG 2 cut(s) 139, 338
BsiHKCI CYCGRG 2 cut(s) 13, 319
BsiSI CCGG 2 cut(s) 210, 310
BslFI GGGAC 2 cut(s) 126, 453
BslI CCNNNNNNNGG 3 cut(s) 191, 476, 477
BsmAI GTCTC 3 cut(s) 251, 306, 738
BsmFI GGGAC 2 cut(s) 126, 453
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 2 cut(s) 13, 319
Bsp1286I GDGCHC 3 cut(s) 20, 123, 525
Bsp143I GATC 3 cut(s) 212, 315, 424
BspACI CCGC 8 cut(s) 60, 63, 86, 192, 231, 278, 407, 489
BspCNI CTCAG 3 cut(s) 109, 706, 748
BspFNI CGCG 2 cut(s) 139, 338
BspHI TCATGA 1 cut(s) 717
BspLI GGNNCC 2 cut(s) 114, 433
BspMAI CTGCAG 1 cut(s) 96
BspMI ACCTGC 2 cut(s) 97, 173
BspPI GGATC 2 cut(s) 220, 323
BspQI GCTCTTC 1 cut(s) 535
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 409
BsrI ACTGG 5 cut(s) 257, 298, 626, 730, 770
BssMI GATC 3 cut(s) 212, 315, 424
BssNI GRCGYC 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 1 cut(s) 535
BstACI GRCGYC 1 cut(s) 281
BstAPI GCANNNNNTGC 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 170, 236
BstDEI CTNAG 3 cut(s) 117, 693, 735
BstF5I GGATG 2 cut(s) 32, 160
BstFNI CGCG 2 cut(s) 139, 338
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 3 cut(s) 215, 318, 427
BstMAI GTCTC 3 cut(s) 251, 306, 738
BstMBI GATC 3 cut(s) 212, 315, 424
BstMWI GCNNNNNNNGC 5 cut(s) 69, 91, 228, 355, 361
BstSFI CTRYAG 1 cut(s) 92
BstSLI GKGCMC 1 cut(s) 525
BstUI CGCG 2 cut(s) 139, 338
BstV1I GCAGC 6 cut(s) 146, 231, 367, 468, 525, 621
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 32, 160
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 210, 777
BveI ACCTGC 2 cut(s) 97, 173
Cac8I GCNNGC 2 cut(s) 170, 236
CaiI CAGNNNCTG 2 cut(s) 304, 700
CciI TCATGA 1 cut(s) 717
CfoI GCGC 1 cut(s) 139
Cfr13I GGNCC 2 cut(s) 113, 162
CseI GACGC 3 cut(s) 270, 327, 344
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviAII CATG 4 cut(s) 262, 641, 718, 766
DdeI CTNAG 3 cut(s) 117, 693, 735
DpnI GATC 3 cut(s) 214, 317, 426
DpnII GATC 3 cut(s) 212, 315, 424
DrdI GACNNNNNNGTC 1 cut(s) 265
DriI GACNNNNNGTC 1 cut(s) 337
DseDI GACNNNNNNGTC 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 535
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 535
Eco24I GRGCYC 2 cut(s) 20, 123
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 2 cut(s) 113, 162
Eco57I CTGAAG 1 cut(s) 158
Eco88I CYCGRG 2 cut(s) 13, 319
EcoO109I RGGNCCY 1 cut(s) 113
EcoT38I GRGCYC 2 cut(s) 20, 123
FaeI CATG 4 cut(s) 265, 644, 721, 769
FaiI YATR 4 cut(s) 263, 642, 719, 767
FaqI GGGAC 2 cut(s) 126, 453
FatI CATG 4 cut(s) 261, 640, 717, 765
FauI CCCGC 1 cut(s) 400
FbaI TGATCA 1 cut(s) 424
FokI GGATG 2 cut(s) 19, 167
FriOI GRGCYC 2 cut(s) 20, 123
FspBI CTAG 1 cut(s) 495
GlaI GCGC 1 cut(s) 138
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 1 cut(s) 218
HapII CCGG 2 cut(s) 210, 310
HgaI GACGC 3 cut(s) 270, 327, 344
HhaI GCGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 4 cut(s) 265, 644, 721, 769
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HinfI GANTC 3 cut(s) 181, 658, 697
HpaII CCGG 2 cut(s) 210, 310
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 2 cut(s) 162, 331
Hpy188I TCNGA 5 cut(s) 271, 452, 657, 696, 702
Hpy188III TCNNGA 4 cut(s) 197, 293, 389, 718
Hpy8I GTNNAC 2 cut(s) 162, 331
Hpy99I CGWCG 3 cut(s) 133, 338, 343
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4V TGCA 2 cut(s) 94, 364
HpyF10VI GCNNNNNNNGC 5 cut(s) 69, 91, 228, 355, 361
HpyF3I CTNAG 3 cut(s) 117, 693, 735
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 4 cut(s) 265, 644, 721, 769
HspAI GCGC 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 3 cut(s) 212, 315, 424
LguI GCTCTTC 1 cut(s) 535
LmnI GCTCC 2 cut(s) 312, 744
Lsp1109I GCAGC 6 cut(s) 146, 231, 367, 468, 525, 621
LweI GCATC 2 cut(s) 41, 218
MaeI CTAG 1 cut(s) 495
MaeIII GTNAC 3 cut(s) 205, 380, 772
MalI GATC 3 cut(s) 214, 317, 426
MboI GATC 3 cut(s) 212, 315, 424
MboII GAAGA 3 cut(s) 18, 276, 522
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 3 cut(s) 20, 123, 525
MluCI AATT 5 cut(s) 436, 472, 544, 604, 613
MluI ACGCGT 1 cut(s) 336
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 8 cut(s) 55, 58, 182, 193, 241, 648, 702, 753
MslI CAYNNNNRTG 1 cut(s) 770
MspA1I CMGCKG 2 cut(s) 60, 358
MspI CCGG 2 cut(s) 210, 310
MvnI CGCG 2 cut(s) 139, 338
MwoI GCNNNNNNNGC 5 cut(s) 69, 91, 228, 355, 361
NdeII GATC 3 cut(s) 212, 315, 424
NlaIII CATG 4 cut(s) 265, 644, 721, 769
NlaIV GGNNCC 2 cut(s) 114, 433
NmuCI GTSAC 2 cut(s) 205, 772
PagI TCATGA 1 cut(s) 717
PaqCI CACCTGC 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 535
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 3 cut(s) 181, 658, 697
PpuMI RGGWCCY 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 524
PspN4I GGNNCC 2 cut(s) 114, 433
PspPI GGNCC 2 cut(s) 113, 162
PspPPI RGGWCCY 1 cut(s) 113
PstI CTGCAG 1 cut(s) 96
PstNI CAGNNNCTG 2 cut(s) 304, 700
PsuI RGATCY 1 cut(s) 315
PvuII CAGCTG 1 cut(s) 358
RseI CAYNNNNRTG 1 cut(s) 770
SapI GCTCTTC 1 cut(s) 535
Sau3AI GATC 3 cut(s) 212, 315, 424
Sau96I GGNCC 2 cut(s) 113, 162
SduI GDGCHC 3 cut(s) 20, 123, 525
SetI ASST 8 cut(s) 111, 118, 167, 174, 360, 530, 555, 741
SfaNI GCATC 2 cut(s) 41, 218
SfcI CTRYAG 1 cut(s) 92
SinI GGWCC 2 cut(s) 113, 162
SmiMI CAYNNNNRTG 1 cut(s) 770
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 5 cut(s) 436, 472, 544, 604, 613
SsiI CCGC 8 cut(s) 60, 63, 86, 192, 231, 278, 407, 489
SspMI CTAG 1 cut(s) 495
TaaI ACNGT 1 cut(s) 380
TaqI TCGA 2 cut(s) 179, 441
TasI AATT 5 cut(s) 436, 472, 544, 604, 613
TauI GCSGC 5 cut(s) 63, 66, 88, 234, 281
TfiI GAWTC 3 cut(s) 181, 658, 697
TscAI CASTG 2 cut(s) 210, 777
TseFI GTSAC 2 cut(s) 205, 772
TseI GCWGC 6 cut(s) 134, 219, 355, 456, 538, 634
Tsp45I GTSAC 2 cut(s) 205, 772
TspDTI ATGAA 4 cut(s) 699, 706, 734, 754
TspRI CASTG 2 cut(s) 210, 777
VpaK11BI GGWCC 2 cut(s) 113, 162
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.