Rmu_sc0004485.1_g000010

Scarecrow-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004485.1
OG0013778
Physical Location & Seq
Forward (+)
65916 .. 66371
456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004485.1_g000010.1.cds

Sequence Viewer

Length: 363 bp
atgatcgcgaattcagaaatcaatttagagattaagagggaaagacctggtggtaatcttcggaaggaacaaccaaggcgtcaagaggaggagagtgagacgccattagagacctatgatacttcgtcggtgggacccgagcccgacacggcggcggcgcgtagcagcagcaaggatgagtggacctgcgagcttcagtcgattcccagcggcggtctggaggccgtgaccggatcgggcggaaggagatacggcgggcttggattggaggacttggagaccatgctgtccgaaacggcggcgtctccgggtcaagaccagtcgctgctctggtggatcttaggcgacgtggacgacgcgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

120

Amino Acids

13.02

Weight (kDa)

4.26

Isoelectric Point (pI)

73.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 194
AccII CGCG 3 cut(s) 8, 160, 359
AciI CCGC 7 cut(s) 152, 155, 210, 213, 240, 255, 299
AclWI GGATC 2 cut(s) 241, 344
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 1 cut(s) 179
AcyI GRCGYC 3 cut(s) 79, 101, 302
AfiI CCNNNNNNNGG 1 cut(s) 212
AflIII ACRYGT 1 cut(s) 357
AjiI CACGTC 1 cut(s) 349
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 1 cut(s) 193
AluI AGCT 1 cut(s) 193
Alw26I GTCTC 4 cut(s) 92, 104, 272, 309
AlwI GGATC 2 cut(s) 241, 344
AlwNI CAGNNNCTG 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 1 cut(s) 222
ApeKI GCWGC 3 cut(s) 165, 168, 325
ApoI RAATTY 1 cut(s) 10
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 2 cut(s) 134, 183
AsuC2I CCSGG 1 cut(s) 309
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 2 cut(s) 134, 183
BanII GRGCYC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 177, 180, 312
BceAI ACGGC 4 cut(s) 165, 209, 268, 312
BciT130I CCWGG 1 cut(s) 48
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 4 cut(s) 92, 104, 272, 309
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 7 cut(s) 153, 156, 166, 169, 211, 300, 326
BlsI GCNGC 7 cut(s) 154, 157, 167, 170, 212, 301, 327
Bme1390I CCNGG 2 cut(s) 48, 309
Bme18I GGWCC 2 cut(s) 134, 183
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 2 cut(s) 134, 183
BmiI GGNNCC 2 cut(s) 135, 136
BmrFI CCNGG 2 cut(s) 48, 309
BpmI CTGGAG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 309
BsaHI GRCGYC 3 cut(s) 79, 101, 302
BsaI GGTCTC 2 cut(s) 104, 272
BsaJI CCNNGG 1 cut(s) 74
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 319
BseRI GAGGAG 2 cut(s) 101, 104
BseXI GCAGC 3 cut(s) 177, 180, 312
BseYI CCCAGC 1 cut(s) 206
Bsh1236I CGCG 3 cut(s) 8, 160, 359
BshFI GGCC 1 cut(s) 224
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 2 cut(s) 231, 308
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 4 cut(s) 92, 104, 272, 309
BsmBI CGTCTC 2 cut(s) 92, 309
BsmFI GGGAC 1 cut(s) 147
BsnI GGCC 1 cut(s) 224
Bso31I GGTCTC 2 cut(s) 104, 272
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 3, 233, 336
Bsp68I TCGCGA 1 cut(s) 8
BspACI CCGC 7 cut(s) 152, 155, 210, 213, 240, 255, 299
BspANI GGCC 1 cut(s) 224
BspFNI CGCG 3 cut(s) 8, 160, 359
BspLI GGNNCC 2 cut(s) 135, 136
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 2 cut(s) 241, 344
BspTNI GGTCTC 2 cut(s) 104, 272
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 74
BssMI GATC 3 cut(s) 3, 233, 336
BssNI GRCGYC 3 cut(s) 79, 101, 302
BssT1I CCWWGG 1 cut(s) 74
Bst2UI CCWGG 1 cut(s) 48
BstACI GRCGYC 3 cut(s) 79, 101, 302
BstC8I GCNNGC 2 cut(s) 191, 257
BstDEI CTNAG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 181
BstFNI CGCG 3 cut(s) 8, 160, 359
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 6, 236, 339
BstMAI GTCTC 4 cut(s) 92, 104, 272, 309
BstMBI GATC 3 cut(s) 3, 233, 336
BstNI CCWGG 1 cut(s) 48
BstSCI CCNGG 2 cut(s) 46, 307
BstUI CGCG 3 cut(s) 8, 160, 359
BstV1I GCAGC 3 cut(s) 177, 180, 312
BstX2I RGATCY 1 cut(s) 336
BstYI RGATCY 1 cut(s) 336
BsuRI GGCC 1 cut(s) 224
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 1 cut(s) 181
BtuMI TCGCGA 1 cut(s) 8
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 191, 257
CaiI CAGNNNCTG 1 cut(s) 325
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 134, 183
CseI GACGC 3 cut(s) 68, 109, 291
CsiI ACCWGGT 1 cut(s) 46
CviAII CATG 1 cut(s) 283
CviJI RGCY 4 cut(s) 142, 193, 224, 259
CviKI_1 RGCY 4 cut(s) 142, 193, 224, 259
DdeI CTNAG 1 cut(s) 340
DpnI GATC 3 cut(s) 5, 235, 338
DpnII GATC 3 cut(s) 3, 233, 336
DrdI GACNNNNNNGTC 1 cut(s) 286
DseDI GACNNNNNNGTC 1 cut(s) 286
EciI GGCGGA 1 cut(s) 255
Eco130I CCWWGG 1 cut(s) 74
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 2 cut(s) 104, 272
Eco47I GGWCC 2 cut(s) 134, 183
Eco57I CTGAAG 1 cut(s) 179
Eco88I CYCGRG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 74
Esp3I CGTCTC 2 cut(s) 92, 309
FaeI CATG 1 cut(s) 286
FaiI YATR 2 cut(s) 117, 284
FaqI GGGAC 1 cut(s) 147
FatI CATG 1 cut(s) 282
FauI CCCGC 1 cut(s) 248
Fnu4HI GCNGC 7 cut(s) 153, 156, 166, 169, 211, 300, 326
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 7 cut(s) 153, 156, 166, 169, 211, 300, 326
GlaI GCGC 1 cut(s) 159
GluI GCNGC 7 cut(s) 153, 156, 166, 169, 211, 300, 326
GsaI CCCAGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 1 cut(s) 224
HapII CCGG 2 cut(s) 231, 308
HgaI GACGC 3 cut(s) 68, 109, 291
HhaI GCGC 1 cut(s) 160
Hin1I GRCGYC 3 cut(s) 79, 101, 302
Hin1II CATG 1 cut(s) 286
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 1 cut(s) 202
HpaII CCGG 2 cut(s) 231, 308
Hpy166II GTNNAC 2 cut(s) 183, 352
Hpy188I TCNGA 3 cut(s) 16, 63, 292
Hpy188III TCNNGA 4 cut(s) 7, 83, 218, 314
Hpy8I GTNNAC 2 cut(s) 183, 352
Hpy99I CGWCG 3 cut(s) 130, 350, 359
HpyAV CCTTC 2 cut(s) 58, 237
HpyCH4IV ACGT 1 cut(s) 348
HpyF3I CTNAG 1 cut(s) 340
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 3 cut(s) 79, 101, 302
Hsp92II CATG 1 cut(s) 286
HspAI GCGC 1 cut(s) 158
KflI GGGWCCC 1 cut(s) 134
Kzo9I GATC 3 cut(s) 3, 233, 336
LpnPI CCDG 9 cut(s) 33, 60, 199, 203, 220, 244, 316, 321, 332
Lsp1109I GCAGC 3 cut(s) 177, 180, 312
MabI ACCWGGT 1 cut(s) 46
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 1 cut(s) 226
MalI GATC 3 cut(s) 5, 235, 338
MboI GATC 3 cut(s) 3, 233, 336
MboII GAAGA 1 cut(s) 50
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 2 cut(s) 10, 22
MluI ACGCGT 1 cut(s) 357
MnlI CCTC 5 cut(s) 30, 79, 82, 214, 262
MseI TTAA 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 2 cut(s) 231, 308
MspR9I CCNGG 2 cut(s) 48, 309
MvaI CCWGG 1 cut(s) 48
MvnI CGCG 3 cut(s) 8, 160, 359
NciI CCSGG 1 cut(s) 309
NdeII GATC 3 cut(s) 3, 233, 336
NlaIII CATG 1 cut(s) 286
NlaIV GGNNCC 2 cut(s) 135, 136
NmuCI GTSAC 1 cut(s) 226
NruI TCGCGA 1 cut(s) 8
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 7 cut(s) 154, 157, 167, 170, 212, 301, 327
PpuMI RGGWCCY 1 cut(s) 134
Psp5II RGGWCCY 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 2 cut(s) 135, 136
PspPI GGNCC 2 cut(s) 134, 183
PspPPI RGGWCCY 1 cut(s) 134
PstNI CAGNNNCTG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 336
RruI TCGCGA 1 cut(s) 8
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 7 cut(s) 153, 156, 166, 169, 211, 300, 326
Sau3AI GATC 3 cut(s) 3, 233, 336
Sau96I GGNCC 2 cut(s) 134, 183
ScrFI CCNGG 2 cut(s) 48, 309
SduI GDGCHC 1 cut(s) 144
SetI ASST 5 cut(s) 49, 116, 188, 195, 351
SexAI ACCWGGT 1 cut(s) 46
SinI GGWCC 2 cut(s) 134, 183
Sse9I AATT 2 cut(s) 10, 22
SsiI CCGC 7 cut(s) 152, 155, 210, 213, 240, 255, 299
StyD4I CCNGG 2 cut(s) 46, 307
StyI CCWWGG 1 cut(s) 74
TaiI ACGT 1 cut(s) 351
TaqI TCGA 1 cut(s) 200
TasI AATT 2 cut(s) 10, 22
TauI GCSGC 4 cut(s) 155, 158, 213, 302
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 3 cut(s) 165, 168, 325
Tsp45I GTSAC 1 cut(s) 226
VpaK11BI GGWCC 2 cut(s) 134, 183
XapI RAATTY 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.