Rmu_sc0000077.1_g000045

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000077.1
OG0002668
Physical Location & Seq
Reverse (-)
176228 .. 182288
6061 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2286 bp
atgcaaggtcaagggagtggaattgatactttttcagaaatcactgatgttggtcttggacctgtttccaacagcactggtgaacctagctctagattgaatttgcaaaacacggtcaatgatggtgggacaagaatggaacatggttggtcggctccatatagtgcttgtccagtgattgctcaaagatcggaagatagacgaattgagccaaatatcctttttcctgggagatctcttattggccttggtggcaatcagataccaggtgggccttctttttcagaaggttctagctctagttatatccctcagcatgtaaatttaaatgcaggatatgttgaaaatagtggtaactatgggcatggtatggaagctgatgcagaccctaaattctacaagtcaagtggattagaaggagaacagacatctcgtggcagtgcttcacatgatattgttgggacttcatctggaagctctgattatatggttggggaagatgatggtaatccaggctcttcctttggcaattggggtttatcctgcaagcgaaaggctcttgagggtacgtcagggcagtcttatcccggtggaagttctagctcattttctcaacctgaaaatcgagcattgaatactggtcctgcatgttataatccttctagtggtttaagtttatctacgccccgtaattctcctagtggcattccttcagagcagcagaactcaagaaccaggattggtttgagagttggagcatctgatacctttccttcattaggtgttacgggaaatgcagagagcccactgagacaatatgttaggggtttctgtagtactgggtctcagcaagaatctgcaccatcaaacttatcatcagctggaagatcaacaacagtagctgcaacaaattcgggtggtcctcaaagccagtctcctgctatgcatattaacagttcgtcaggaaacatgcttcctttcccttggaatggtgcttccagttcacgagtgggcagcttatcaggctctgttatgtccggatatagaggtgctgagttaagagaagaagcaagctcacgaagcatttctagaaacaatgtggagcaccccatgtttgtagctgttactgatatgagaaattcagcacaggatccaacaggttggagtttagccccgggaaacttaagcacttctgtaggaggtccttcctctgctagaattggctccagttccagcattcattctttgcctaatccctcatggatccctaacatccctccaacagaaaatcaaaggataactgaatttgcaccttggtctttgtttccatctatagattctcaacccggaggtcataatcattataatccaataccatcaggaccttctgcttcctctcaggattctggaacttcttctggatcaaataatcagggtcgtcaacaatcacatcctaggtcagcattcttgttggacagacaaggtgatgatgttcttaatatgccccattcactacgggcgttagcagctgacattgatgggaggcgccgacttatatctgagattcgtcaagttttgaatgctatgcgcaggggtgagaacttacgagtcgaggattatatgctctttgacccattcatatatcatggcatggccgagatgcatgataggcacagagacatgcgccttgatgttgataacatgtcttacgaggaactgttagcattggaggaacgtataggagatgttagcactggactgagtgaggagaccatattgaaattgatgaaacagagaaaatgtctgtctgttacaacagaattcccagcagatatggaaccctgctgtatctgtcaggagcaatatgctgatggggatgacattggcagactggattgtgggcacgacttccacaccaactgcattaaacagtggctaatgcagaagaatctgtgcccgatttatcgagcagagcgagaaccactaggaagtgaggagaacttgctaaagcgtgactttagcttggctgggttgctagggcgttacccttgcttggctggttctgtaaccgttgtggtcgcggcactaccgtcggctcccgaggagactaggaccgaaccattgacattggctcaatttctccaagacgtgccttatcaggccaaaatgctcattttgggtccaaacaatgagctcattctagttgacgtccatagttgtgaaaattcttctacccacctaggtgggtggtcacctaatgcgttgtcaggagatctagctttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

761

Amino Acids

81.44

Weight (kDa)

5.22

Isoelectric Point (pI)

61.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 612 - 654 1.7e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 615 - 654 3.9e-11 Ring finger domain
zf-C3HC4_2 PF13923 615 - 654 5.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 615 - 642 8.8e-06 RING-like zinc finger
zf-C3HC4 PF00097 615 - 653 1e-05 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 654, 1365
AatII GACGTC 1 cut(s) 2211
Acc16I TGCGCA 1 cut(s) 1589
AccB1I GGYRCC 1 cut(s) 1545
AccII CGCG 1 cut(s) 2081
AccIII TCCGGA 1 cut(s) 1037
AciI CCGC 1 cut(s) 2081
AclWI GGATC 5 cut(s) 1145, 1158, 1258, 1271, 1429
AcoI YGGCCR 1 cut(s) 1653
AcsI RAATTY 8 cut(s) 100, 322, 392, 910, 1138, 1304, 1820, 2224
AcuI CTGAAG 1 cut(s) 696
AcyI GRCGYC 2 cut(s) 1546, 2208
AfaI GTAC 2 cut(s) 568, 838
AfiI CCNNNNNNNGG 4 cut(s) 665, 779, 989, 2053
AflII CTTAAG 1 cut(s) 1183
AflIII ACRYGT 1 cut(s) 1701
AgsI TTSAA 5 cut(s) 100, 344, 634, 1579, 1780
AjiI CACGTC 1 cut(s) 2149
AjnI CCWGG 4 cut(s) 226, 265, 511, 734
AleI CACNNNNGTG 1 cut(s) 2241
Alw21I GWGCWC 2 cut(s) 1107, 2196
Alw26I GTCTC 6 cut(s) 805, 849, 939, 1671, 1763, 2099
AlwI GGATC 5 cut(s) 1145, 1158, 1258, 1271, 1429
AlwNI CAGNNNCTG 2 cut(s) 902, 1028
Ama87I CYCGRG 2 cut(s) 1174, 2099
Aor13HI TCCGGA 1 cut(s) 1037
AoxI GGCC 4 cut(s) 244, 272, 1653, 2160
ApeKI GCWGC 4 cut(s) 718, 902, 1014, 1526
ApoI RAATTY 8 cut(s) 100, 322, 392, 910, 1138, 1304, 1820, 2224
Asp700I GAANNNNTTC 2 cut(s) 1084, 1414
AspA2I CCTAGG 2 cut(s) 1454, 2239
AspLEI GCGC 3 cut(s) 1548, 1590, 1686
AspS9I GGNCC 8 cut(s) 59, 272, 641, 920, 1202, 1382, 2112, 2180
AsuC2I CCSGG 4 cut(s) 588, 1175, 1176, 1347
AsuHPI GGTGA 4 cut(s) 92, 1496, 1607, 2244
AvaI CYCGRG 2 cut(s) 1174, 2099
AvaII GGWCC 7 cut(s) 59, 641, 920, 1202, 1382, 2112, 2180
AvrII CCTAGG 2 cut(s) 1454, 2239
BaeGI GKGCMC 2 cut(s) 1905, 1958
BamHI GGATCC 2 cut(s) 1150, 1263
BanI GGYRCC 1 cut(s) 1545
BanII GRGCYC 2 cut(s) 806, 2196
BauI CACGAG 2 cut(s) 432, 1005
Bbv12I GWGCWC 2 cut(s) 1107, 2196
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 4 cut(s) 730, 889, 1026, 1538
BccI CCATC 7 cut(s) 116, 497, 871, 1336, 1384, 1532, 1865
BcgI CGANNNNNNTGC 4 cut(s) 894, 928, 2073, 2107
BciT130I CCWGG 4 cut(s) 228, 267, 513, 736
BcnI CCSGG 4 cut(s) 588, 1175, 1176, 1347
BcoDI GTCTC 6 cut(s) 805, 849, 939, 1671, 1763, 2099
BfmI CTRYAG 3 cut(s) 832, 1194, 1332
BfoI RGCGCY 1 cut(s) 1549
BfrI CTTAAG 1 cut(s) 1183
BglI GCCNNNNNGGC 1 cut(s) 252
BglII AGATCT 2 cut(s) 233, 2272
BisI GCNGC 5 cut(s) 719, 903, 1015, 1527, 2082
BlnI CCTAGG 2 cut(s) 1454, 2239
BlsI GCNGC 5 cut(s) 720, 904, 1016, 1528, 2083
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 8 cut(s) 228, 267, 513, 588, 736, 1175, 1176, 1347
Bme18I GGWCC 7 cut(s) 59, 641, 920, 1202, 1382, 2112, 2180
BmeT110I CYCGRG 2 cut(s) 1174, 2099
BmgBI CACGTC 1 cut(s) 2149
BmgT120I GGNCC 8 cut(s) 59, 272, 641, 920, 1202, 1382, 2112, 2180
BmiI GGNNCC 8 cut(s) 156, 1152, 1225, 1265, 1547, 1839, 2097, 2181
BmrFI CCNGG 8 cut(s) 228, 267, 513, 588, 736, 1175, 1176, 1347
BmrI ACTGGG 1 cut(s) 849
BmsI GCATC 3 cut(s) 370, 767, 1650
BmuI ACTGGG 1 cut(s) 849
BpmI CTGGAG 1 cut(s) 1210
Bpu10I CCTNAGC 1 cut(s) 312
BpuEI CTTGAG 2 cut(s) 581, 712
BpuMI CCSGG 4 cut(s) 588, 1175, 1176, 1347
BsaBI GATNNNNATC 1 cut(s) 507
BsaHI GRCGYC 2 cut(s) 1546, 2208
BsaI GGTCTC 2 cut(s) 849, 1763
BsaJI CCNNGG 9 cut(s) 227, 247, 983, 1173, 1174, 1313, 1454, 2100, 2239
BsaWI WCCGGW 1 cut(s) 1037
Bsc4I CCNNNNNNNGG 4 cut(s) 665, 779, 989, 2053
Bse1I ACTGG 9 cut(s) 82, 173, 643, 844, 931, 999, 1227, 1759, 1896
Bse8I GATNNNNATC 1 cut(s) 507
BseAI TCCGGA 1 cut(s) 1037
BseBI CCWGG 4 cut(s) 228, 267, 513, 736
BseDI CCNNGG 9 cut(s) 227, 247, 983, 1173, 1174, 1313, 1454, 2100, 2239
BseGI GGATG 3 cut(s) 1272, 1450, 1882
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 4 cut(s) 665, 779, 989, 2053
BseMII CTCAG 7 cut(s) 326, 800, 860, 1044, 1412, 1551, 1751
BseNI ACTGG 9 cut(s) 82, 173, 643, 844, 931, 999, 1227, 1759, 1896
BseRI GAGGAG 3 cut(s) 1781, 2009, 2117
BseSI GKGCMC 2 cut(s) 1905, 1958
BseXI GCAGC 4 cut(s) 730, 889, 1026, 1538
BseYI CCCAGC 2 cut(s) 1825, 2027
BsgI GTGCAG 1 cut(s) 843
Bsh1236I CGCG 1 cut(s) 2081
BshFI GGCC 4 cut(s) 246, 274, 1655, 2162
BshNI GGYRCC 1 cut(s) 1545
BsiHKAI GWGCWC 2 cut(s) 1107, 2196
BsiHKCI CYCGRG 2 cut(s) 1174, 2099
BsiSI CCGG 4 cut(s) 588, 1038, 1175, 1347
BslFI GGGAC 2 cut(s) 142, 475
BslI CCNNNNNNNGG 4 cut(s) 665, 779, 989, 2053
BsmAI GTCTC 6 cut(s) 805, 849, 939, 1671, 1763, 2099
BsmFI GGGAC 2 cut(s) 142, 475
BsmI GAATGC 4 cut(s) 705, 1236, 1463, 1585
BsnI GGCC 4 cut(s) 246, 274, 1655, 2162
Bso31I GGTCTC 2 cut(s) 849, 1763
BsoBI CYCGRG 2 cut(s) 1174, 2099
Bsp1286I GDGCHC 5 cut(s) 806, 1107, 1905, 1958, 2196
Bsp13I TCCGGA 1 cut(s) 1037
Bsp143I GATC 7 cut(s) 188, 233, 887, 1150, 1263, 1421, 2272
BspACI CCGC 1 cut(s) 2081
BspANI GGCC 4 cut(s) 246, 274, 1655, 2162
BspCNI CTCAG 7 cut(s) 325, 801, 859, 1045, 1411, 1552, 1752
BspEI TCCGGA 1 cut(s) 1037
BspFNI CGCG 1 cut(s) 2081
BspLI GGNNCC 8 cut(s) 156, 1152, 1225, 1265, 1547, 1839, 2097, 2181
BspPI GGATC 5 cut(s) 1145, 1158, 1258, 1271, 1429
BspQI GCTCTTC 1 cut(s) 523
BspT107I GGYRCC 1 cut(s) 1545
BspTI CTTAAG 1 cut(s) 1183
BspTNI GGTCTC 2 cut(s) 849, 1763
BsrI ACTGG 9 cut(s) 82, 173, 643, 844, 931, 999, 1227, 1759, 1896
BssECI CCNNGG 9 cut(s) 227, 247, 983, 1173, 1174, 1313, 1454, 2100, 2239
BssMI GATC 7 cut(s) 188, 233, 887, 1150, 1263, 1421, 2272
BssNI GRCGYC 2 cut(s) 1546, 2208
BssSI CACGAG 2 cut(s) 432, 1005
BssT1I CCWWGG 5 cut(s) 247, 983, 1313, 1454, 2239
Bst2BI CACGAG 2 cut(s) 432, 1005
Bst2UI CCWGG 4 cut(s) 228, 267, 513, 736
Bst4CI ACNGT 7 cut(s) 115, 898, 956, 1719, 1932, 2071, 2091
Bst6I CTCTTC 1 cut(s) 523
BstACI GRCGYC 2 cut(s) 1546, 2208
BstAFI CTTAAG 1 cut(s) 1183
BstC8I GCNNGC 2 cut(s) 548, 1072
BstDEI CTNAG 7 cut(s) 312, 809, 846, 1053, 1398, 1560, 1760
BstEII GGTNACC 1 cut(s) 2250
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 3 cut(s) 1272, 1450, 1882
BstFNI CGCG 1 cut(s) 2081
BstH2I RGCGCY 1 cut(s) 1549
BstHHI GCGC 3 cut(s) 1548, 1590, 1686
BstKTI GATC 7 cut(s) 191, 236, 890, 1153, 1266, 1424, 2275
BstMAI GTCTC 6 cut(s) 805, 849, 939, 1671, 1763, 2099
BstMBI GATC 7 cut(s) 188, 233, 887, 1150, 1263, 1421, 2272
BstMWI GCNNNNNNNGC 5 cut(s) 252, 1023, 1080, 1526, 1669
BstNI CCWGG 4 cut(s) 228, 267, 513, 736
BstNSI RCATGY 5 cut(s) 320, 651, 973, 1684, 1705
BstPI GGTNACC 1 cut(s) 2250
BstSCI CCNGG 8 cut(s) 226, 265, 511, 586, 734, 1173, 1174, 1345
BstSFI CTRYAG 3 cut(s) 832, 1194, 1332
BstSLI GKGCMC 2 cut(s) 1905, 1958
BstUI CGCG 1 cut(s) 2081
BstV1I GCAGC 4 cut(s) 730, 889, 1026, 1538
BstX2I RGATCY 4 cut(s) 233, 1150, 1263, 2272
BstXI CCANNNNNNTGG 2 cut(s) 1161, 2243
BstYI RGATCY 4 cut(s) 233, 1150, 1263, 2272
BsuRI GGCC 4 cut(s) 246, 274, 1655, 2162
BtrI CACGTC 1 cut(s) 2149
BtsCI GGATG 3 cut(s) 1272, 1450, 1882
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 7 cut(s) 42, 75, 180, 445, 806, 1752, 1937
Cac8I GCNNGC 2 cut(s) 548, 1072
CaiI CAGNNNCTG 2 cut(s) 902, 1028
CfoI GCGC 3 cut(s) 1548, 1590, 1686
Cfr13I GGNCC 8 cut(s) 59, 272, 641, 920, 1202, 1382, 2112, 2180
Cfr9I CCCGGG 1 cut(s) 1174
CsiI ACCWGGT 1 cut(s) 265
Csp6I GTAC 2 cut(s) 567, 837
CspCI CAANNNNNGTGG 4 cut(s) 106, 141, 388, 423
CviQI GTAC 2 cut(s) 567, 837
DdeI CTNAG 7 cut(s) 312, 809, 846, 1053, 1398, 1560, 1760
DinI GGCGCC 1 cut(s) 1547
DpnI GATC 7 cut(s) 190, 235, 889, 1152, 1265, 1423, 2274
DpnII GATC 7 cut(s) 188, 233, 887, 1150, 1263, 1421, 2272
DraI TTTAAA 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 1653
Eam1104I CTCTTC 1 cut(s) 523
EarI CTCTTC 1 cut(s) 523
Ecl136II GAGCTC 1 cut(s) 2194
Eco130I CCWWGG 5 cut(s) 247, 983, 1313, 1454, 2239
Eco24I GRGCYC 2 cut(s) 806, 2196
Eco31I GGTCTC 2 cut(s) 849, 1763
Eco47I GGWCC 7 cut(s) 59, 641, 920, 1202, 1382, 2112, 2180
Eco53kI GAGCTC 1 cut(s) 2194
Eco57I CTGAAG 1 cut(s) 696
Eco88I CYCGRG 2 cut(s) 1174, 2099
Eco91I GGTNACC 1 cut(s) 2250
EcoICRI GAGCTC 1 cut(s) 2194
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoO109I RGGNCCY 2 cut(s) 1202, 1382
EcoO65I GGTNACC 1 cut(s) 2250
EcoRI GAATTC 1 cut(s) 1820
EcoRII CCWGG 4 cut(s) 226, 265, 511, 734
EcoT14I CCWWGG 5 cut(s) 247, 983, 1313, 1454, 2239
EcoT22I ATGCAT 2 cut(s) 948, 1665
EcoT38I GRGCYC 2 cut(s) 806, 2196
EgeI GGCGCC 1 cut(s) 1547
EheI GGCGCC 1 cut(s) 1547
ErhI CCWWGG 5 cut(s) 247, 983, 1313, 1454, 2239
FalI AAGNNNNNCTT 2 cut(s) 2000, 2032
FaqI GGGAC 2 cut(s) 142, 475
Fnu4HI GCNGC 5 cut(s) 719, 903, 1015, 1527, 2082
FokI GGATG 3 cut(s) 1259, 1437, 1889
FriOI GRGCYC 2 cut(s) 806, 2196
Fsp4HI GCNGC 5 cut(s) 719, 903, 1015, 1527, 2082
FspI TGCGCA 1 cut(s) 1589
GlaI GCGC 3 cut(s) 1547, 1589, 1685
GluI GCNGC 5 cut(s) 719, 903, 1015, 1527, 2082
GsaI CCCAGC 2 cut(s) 1829, 2031
GsuI CTGGAG 1 cut(s) 1210
HaeII RGCGCY 1 cut(s) 1549
HaeIII GGCC 4 cut(s) 246, 274, 1655, 2162
HapII CCGG 4 cut(s) 588, 1038, 1175, 1347
HhaI GCGC 3 cut(s) 1548, 1590, 1686
Hin1I GRCGYC 2 cut(s) 1546, 2208
Hin6I GCGC 3 cut(s) 1546, 1588, 1684
HinP1I GCGC 3 cut(s) 1546, 1588, 1684
HincII GTYRAC 2 cut(s) 1442, 2206
HindII GTYRAC 2 cut(s) 1442, 2206
HinfI GANTC 6 cut(s) 854, 1337, 1403, 1564, 1608, 1948
HpaII CCGG 4 cut(s) 588, 1038, 1175, 1347
HphI GGTGA 4 cut(s) 92, 1496, 1607, 2244
Hpy166II GTNNAC 4 cut(s) 83, 1004, 1442, 2206
Hpy188I TCNGA 9 cut(s) 37, 193, 261, 286, 481, 715, 763, 1561, 2285
Hpy8I GTNNAC 4 cut(s) 83, 1004, 1442, 2206
Hpy99I CGWCG 1 cut(s) 2095
HpyAV CCTTC 8 cut(s) 281, 285, 410, 669, 720, 783, 1215, 1395
HpyCH4III ACNGT 7 cut(s) 115, 898, 956, 1719, 1932, 2071, 2091
HpyCH4IV ACGT 4 cut(s) 569, 1735, 2148, 2208
HpyF10VI GCNNNNNNNGC 5 cut(s) 252, 1023, 1080, 1526, 1669
HpyF3I CTNAG 7 cut(s) 312, 809, 846, 1053, 1398, 1560, 1760
HpySE526I ACGT 4 cut(s) 569, 1735, 2148, 2208
Hsp92I GRCGYC 2 cut(s) 1546, 2208
HspAI GCGC 3 cut(s) 1546, 1588, 1684
KasI GGCGCC 1 cut(s) 1545
Kpn2I TCCGGA 1 cut(s) 1037
Kzo9I GATC 7 cut(s) 188, 233, 887, 1150, 1263, 1421, 2272
LguI GCTCTTC 1 cut(s) 523
LmnI GCTCC 6 cut(s) 160, 755, 1102, 1229, 1858, 2101
Lsp1109I GCAGC 4 cut(s) 730, 889, 1026, 1538
LweI GCATC 3 cut(s) 370, 767, 1650
MabI ACCWGGT 1 cut(s) 265
MaeII ACGT 4 cut(s) 569, 1735, 2148, 2208
MaeIII GTNAC 8 cut(s) 353, 784, 1123, 1810, 2012, 2042, 2065, 2250
MalI GATC 7 cut(s) 190, 235, 889, 1152, 1265, 1423, 2274
MboI GATC 7 cut(s) 188, 233, 887, 1150, 1263, 1421, 2272
MboII GAAGA 8 cut(s) 206, 509, 510, 897, 1076, 1407, 1957, 2220
MfeI CAATTG 1 cut(s) 529
MflI RGATCY 4 cut(s) 233, 1150, 1263, 2272
MhlI GDGCHC 5 cut(s) 806, 1107, 1905, 1958, 2196
Mly113I GGCGCC 1 cut(s) 1546
MlyI GAGTC 1 cut(s) 1617
MmeI TCCRAC 6 cut(s) 93, 733, 1142, 1178, 1304, 1452
Mph1103I ATGCAT 2 cut(s) 948, 1665
MroI TCCGGA 1 cut(s) 1037
MroXI GAANNNNTTC 2 cut(s) 1084, 1414
MseI TTAA 7 cut(s) 326, 671, 951, 1058, 1184, 1497, 1926
MslI CAYNNNNRTG 2 cut(s) 2217, 2241
MspA1I CMGCKG 2 cut(s) 881, 1529
MspCI CTTAAG 1 cut(s) 1183
MspI CCGG 4 cut(s) 588, 1038, 1175, 1347
MspR9I CCNGG 8 cut(s) 228, 267, 513, 588, 736, 1175, 1176, 1347
MunI CAATTG 1 cut(s) 529
Mva1269I GAATGC 4 cut(s) 705, 1236, 1463, 1585
MvaI CCWGG 4 cut(s) 228, 267, 513, 736
MvnI CGCG 1 cut(s) 2081
MwoI GCNNNNNNNGC 5 cut(s) 252, 1023, 1080, 1526, 1669
NarI GGCGCC 1 cut(s) 1546
NciI CCSGG 4 cut(s) 588, 1175, 1176, 1347
NdeII GATC 7 cut(s) 188, 233, 887, 1150, 1263, 1421, 2272
NlaIV GGNNCC 8 cut(s) 156, 1152, 1225, 1265, 1547, 1839, 2097, 2181
NmeAIII GCCGAG 1 cut(s) 1681
NmuCI GTSAC 2 cut(s) 2012, 2250
NsbI TGCGCA 1 cut(s) 1589
NsiI ATGCAT 2 cut(s) 948, 1665
NspI RCATGY 5 cut(s) 320, 651, 973, 1684, 1705
OliI CACNNNNGTG 1 cut(s) 2241
PciI ACATGT 1 cut(s) 1701
PciSI GCTCTTC 1 cut(s) 523
PcsI WCGNNNNNNNCGW 1 cut(s) 1972
PctI GAATGC 4 cut(s) 705, 1236, 1463, 1585
PdmI GAANNNNTTC 2 cut(s) 1084, 1414
PfeI GAWTC 5 cut(s) 854, 1337, 1403, 1564, 1948
PkrI GCNGC 5 cut(s) 720, 904, 1016, 1528, 2083
PleI GAGTC 1 cut(s) 1616
PluTI GGCGCC 1 cut(s) 1549
PpsI GAGTC 1 cut(s) 1616
PpuMI RGGWCCY 2 cut(s) 1202, 1382
PscI ACATGT 1 cut(s) 1701
PsiI TTATAA 2 cut(s) 654, 1365
Psp124BI GAGCTC 1 cut(s) 2196
Psp5II RGGWCCY 2 cut(s) 1202, 1382
Psp6I CCWGG 4 cut(s) 226, 265, 511, 734
PspEI GGTNACC 1 cut(s) 2250
PspFI CCCAGC 2 cut(s) 1825, 2027
PspGI CCWGG 4 cut(s) 226, 265, 511, 734
PspN4I GGNNCC 8 cut(s) 156, 1152, 1225, 1265, 1547, 1839, 2097, 2181
PspPI GGNCC 8 cut(s) 59, 272, 641, 920, 1202, 1382, 2112, 2180
PspPPI RGGWCCY 2 cut(s) 1202, 1382
PstNI CAGNNNCTG 2 cut(s) 902, 1028
PsuI RGATCY 4 cut(s) 233, 1150, 1263, 2272
PvuII CAGCTG 2 cut(s) 881, 1529
RsaI GTAC 2 cut(s) 568, 838
RsaNI GTAC 2 cut(s) 567, 837
RseI CAYNNNNRTG 2 cut(s) 2217, 2241
SacI GAGCTC 1 cut(s) 2196
SapI GCTCTTC 1 cut(s) 523
SaqAI TTAA 7 cut(s) 326, 671, 951, 1058, 1184, 1497, 1926
SatI GCNGC 5 cut(s) 719, 903, 1015, 1527, 2082
Sau3AI GATC 7 cut(s) 188, 233, 887, 1150, 1263, 1421, 2272
Sau96I GGNCC 8 cut(s) 59, 272, 641, 920, 1202, 1382, 2112, 2180
ScaI AGTACT 1 cut(s) 838
SchI GAGTC 1 cut(s) 1617
ScrFI CCNGG 8 cut(s) 228, 267, 513, 588, 736, 1175, 1176, 1347
SduI GDGCHC 5 cut(s) 806, 1107, 1905, 1958, 2196
SexAI ACCWGGT 1 cut(s) 265
SfaNI GCATC 3 cut(s) 370, 767, 1650
SfcI CTRYAG 3 cut(s) 832, 1194, 1332
SfoI GGCGCC 1 cut(s) 1547
SinI GGWCC 7 cut(s) 59, 641, 920, 1202, 1382, 2112, 2180
SmaI CCCGGG 1 cut(s) 1176
SmiI ATTTAAAT 1 cut(s) 327
SmiMI CAYNNNNRTG 2 cut(s) 2217, 2241
SmlI CTYRAG 3 cut(s) 560, 727, 1183
SmoI CTYRAG 3 cut(s) 560, 727, 1183
SsiI CCGC 1 cut(s) 2081
SspDI GGCGCC 1 cut(s) 1545
SstI GAGCTC 1 cut(s) 2196
StyD4I CCNGG 8 cut(s) 226, 265, 511, 586, 734, 1173, 1174, 1345
StyI CCWWGG 5 cut(s) 247, 983, 1313, 1454, 2239
SwaI ATTTAAAT 1 cut(s) 327
TaaI ACNGT 7 cut(s) 115, 898, 956, 1719, 1932, 2071, 2091
TaiI ACGT 4 cut(s) 572, 1738, 2151, 2211
TaqI TCGA 3 cut(s) 625, 1611, 1966
TaqII GACCGA 1 cut(s) 2129
TatI WGTACW 1 cut(s) 836
TauI GCSGC 1 cut(s) 2084
TfiI GAWTC 5 cut(s) 854, 1337, 1403, 1564, 1948
Tru1I TTAA 7 cut(s) 326, 671, 951, 1058, 1184, 1497, 1926
Tru9I TTAA 7 cut(s) 326, 671, 951, 1058, 1184, 1497, 1926
TscAI CASTG 7 cut(s) 49, 82, 180, 445, 813, 1759, 1937
TseFI GTSAC 2 cut(s) 2012, 2250
TseI GCWGC 4 cut(s) 718, 902, 1014, 1526
Tsp45I GTSAC 2 cut(s) 2012, 2250
TspDTI ATGAA 5 cut(s) 456, 765, 1229, 1627, 1802
TspMI CCCGGG 1 cut(s) 1174
TspRI CASTG 7 cut(s) 49, 82, 180, 445, 813, 1759, 1937
Vha464I CTTAAG 1 cut(s) 1183
VpaK11BI GGWCC 7 cut(s) 59, 641, 920, 1202, 1382, 2112, 2180
XagI CCTNNNNNAGG 1 cut(s) 777
XapI RAATTY 8 cut(s) 100, 322, 392, 910, 1138, 1304, 1820, 2224
XbaI TCTAGA 2 cut(s) 92, 1088
XceI RCATGY 5 cut(s) 320, 651, 973, 1684, 1705
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XmaI CCCGGG 1 cut(s) 1174
XmaJI CCTAGG 2 cut(s) 1454, 2239
XmnI GAANNNNTTC 2 cut(s) 1084, 1414
ZraI GACGTC 1 cut(s) 2209
ZrmI AGTACT 1 cut(s) 838
Zsp2I ATGCAT 2 cut(s) 948, 1665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.