Rmu_sc0000117.1_g000033

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000117.1
OG0009713
Physical Location & Seq
Reverse (-)
165723 .. 170835
5113 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3015 bp
atgcaacctaggaggcacgagagtttcaatagacagcagcctccggcttctaaattacgtcccacctaccgattcgaatccggtgccaacaaccacccttatagtagtggtagtaggaggagacctgaggaggtacatcggaatattaggaggagtttgagcccgcaacaagatcatctgggtgtcagtcaaaggaatgttgcttctattcagagggagtatggctggcatttgggtggcggtaggactgatggcgtccgccgcaggtcaacatccgggtcacccctactgccttttggtgaaatgcgaaaaatgtcacaatttgaggaggaggaggatggggatttgcggaggagttattcaccaccaccacaaccaccggtacctgttccggcatctgttcctttggagttaaaacacagacgtgatagcgttgaggctggaaattatagtgtcaatgatgatttgaatagtaggagagtttatggctccgagcataatgattttggaatcagtaaagaagatttgaatgaaagcagattgacagctggtggtaaacatgggacattgggtcaaaaaccaatccatatggaagatagtgctgtaagaggatccaaaaccatgtttatcaaaggtgacaccgtgcatggagcatatcattcttctccaagccttggtactgttataactcaaggagcaaggggtggccacctaccatcatcaacgcgcagcatgagcatacgccattctgaccaagaaaggcttcagtatgcagaccctatatctttggatagattaccagtgacagaagcctacaaagaaggaaataaacccgtttttcctacaagagatggcttttattccatgatgtctggatctcattccaagaattattttgctagctcctcaacagggttgaggaatgaattccaagattcctatcagcatgagcagcatatgccttctttggaagaattttcaagaagtagtagaaaacttaaagattctgtgagcattaatgcatatagggaaaggccggttgtagagttttctcgagaccctgattctgggcagaagaatctgacattttaccagagagatagtcctactagagttgagtatgatgatgattattatttctacccaaactcaagaggaatgatatttgacgatcgtgagcttccatctgatcacttacacaaagtgatgcatccacgagcaccacttgactatgaccattctcggatgggttatgatcatagaactgtgtcaagatctagtaccatgctccctgttgtggaaagaattgataatactgaggattattctggaaattcaaggaagcgcattatgctgaataattctactttagaaaggcatacactttcagactaccctgatataagcagaatatcaagtgcatcaaagcagagtggagaatatatgggttctggacgcatgcatgacaagtttggaaggagaatttcacaagattatgaaacatcgcatttgggtgcagcgcaagtttgtcaaatctcacatctgaaagaagattatggttatgaaagagatggaaacctgaagtttcaggacagacagtgtcctgtgtctaaatacgacccagaaatgcatagaaatacgattagaatgcagagtatgagagacaagcttggggtatatgaaccatcagatagggtggtgaaaagaaaatatgttaatgaagagcatggaagtatccagaatcctagagaaattatgtcaggtaaatggaatacctctagagagtttcaagatttgtatgatagtggtgaagagtggaatggtgaagatgtaggatctatgtatacatctagatcagcaagattgggccataatgaacactataaggcccagaggaaatatgctggaatagaccagtatgatgaatttgtatctgatgatcgcctgccgtctcaagactcgttggcagatgcacaaaggcactcagttagatattataaacatggtgatcggtatgccaagggtcaccaaaactatggttcattaagtaggcataggtcacagcaagttgacataaaaagtggctttcacaaacagcacaaggtttggaagagaaatgataattatctagaggatgtccacagaggtgatgataatgatgctgatacatcagaaaatgggttgagttctgcagctcctgagccatcagaggattctgaggagtttatgcaattggtacaagaagccttcctaacatactctaaacaattaaatatgaaccctggtgttcggagaagatacatggaacaaggaaaggctgggactttattctgcatcgtatgtggccgaagctactcgaaggagttcatggacacccaacgattggtaacacatgcatttatgtctaaaaaagctggattgaggacacaacacttgggacttctcaaagcagtttgtgctctgttggggtggagtactgttatccccactgacatagtactatgggttccccaggtcttgcccaaggcagaagctttggctcagaaggaagatttgattctttggcctccagtgattattatccacaatatttctatgtcagacaacaatcctgaaaattggaaggttgtaagcattgaagctctggaggcttttctaagaggtaaaggtttaattaggggaagaatcaagatgtgcctggggaagcctgctgatcaaagtgttgtgttggtgaagttcttgggaactttcactgggcttgggaatgcagagaggattcataagtattttgcagagcagaaccgtggcagagcagactttgagcgagcaacgtccaacaacggaaaaattgtggaagcaggaatgcagggagacagtgtggaggagcgatttttacatggatatatggggattgcggaggacttagataaagtagattttaataccaggaactggagtttgataaagagcaagaaggagattcaggacctggccaatgctcctgttaaaccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1004

Amino Acids

114.75

Weight (kDa)

8.47

Isoelectric Point (pI)

61.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 850 - 976 1.9e-18 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 686, 1998
Acc36I ACCTGC 1 cut(s) 255
Acc65I GGTACC 1 cut(s) 382
AccB1I GGYRCC 2 cut(s) 83, 382
AccB7I CCANNNNNTGG 3 cut(s) 674, 2383, 2952
AccI GTMKAC 1 cut(s) 1844
AccII CGCG 1 cut(s) 727
AciI CCGC 6 cut(s) 164, 240, 259, 262, 349, 2915
AclWI GGATC 4 cut(s) 606, 619, 883, 1843
AcoI YGGCCR 3 cut(s) 706, 2344, 2991
AcsI RAATTY 5 cut(s) 926, 974, 1333, 1482, 1925
AcuI CTGAAG 2 cut(s) 749, 1601
AcyI GRCGYC 1 cut(s) 255
AdeI CACNNNGTG 1 cut(s) 1204
AfaI GTAC 7 cut(s) 135, 384, 679, 1282, 2238, 2476, 2499
AfiI CCNNNNNNNGG 6 cut(s) 674, 912, 1067, 1297, 2383, 2952
AgeI ACCGGT 1 cut(s) 379
AgsI TTSAA 7 cut(s) 28, 469, 529, 981, 1338, 1790, 2639
AhdI GACNNNNNGTC 1 cut(s) 570
AjiI CACGTC 1 cut(s) 425
AjnI CCWGG 5 cut(s) 2281, 2511, 2697, 2945, 2988
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
AleI CACNNNNGTG 2 cut(s) 423, 2145
AluBI AGCT 9 cut(s) 548, 903, 1180, 1669, 2195, 2352, 2414, 2534, 2642
AluI AGCT 9 cut(s) 548, 903, 1180, 1669, 2195, 2352, 2414, 2534, 2642
Alw21I GWGCWC 2 cut(s) 1222, 2461
Alw26I GTCTC 5 cut(s) 115, 1050, 1656, 1956, 2865
AlwI GGATC 4 cut(s) 606, 619, 883, 1843
AlwNI CAGNNNCTG 3 cut(s) 2198, 2952, 2989
Ama87I CYCGRG 1 cut(s) 1053
AoxI GGCC 7 cut(s) 706, 1034, 1867, 1887, 2344, 2564, 2991
ApeKI GCWGC 5 cut(s) 37, 729, 952, 1517, 2192
ApoI RAATTY 5 cut(s) 926, 974, 1333, 1482, 1925
AseI ATTAAT 1 cut(s) 1017
AsiGI ACCGGT 1 cut(s) 379
Asp700I GAANNNNTTC 3 cut(s) 762, 926, 2363
Asp718I GGTACC 1 cut(s) 382
AspA2I CCTAGG 1 cut(s) 8
AspLEI GCGC 3 cut(s) 729, 1347, 1522
AspS9I GGNCC 3 cut(s) 1867, 1888, 2986
AsuC2I CCSGG 1 cut(s) 277
AsuII TTCGAA 1 cut(s) 75
AsuNHI GCTAGC 1 cut(s) 899
AvaI CYCGRG 1 cut(s) 1053
AvaII GGWCC 1 cut(s) 2986
AvrII CCTAGG 1 cut(s) 8
AxyI CCTNAGG 1 cut(s) 126
BalI TGGCCA 2 cut(s) 708, 2993
BamHI GGATCC 1 cut(s) 611
BanI GGYRCC 2 cut(s) 83, 382
BanII GRGCYC 1 cut(s) 164
BauI CACGAG 2 cut(s) 17, 1215
Bbv12I GWGCWC 2 cut(s) 1222, 2461
BbvI GCAGC 5 cut(s) 49, 741, 964, 1529, 2204
BccI CCATC 9 cut(s) 245, 332, 724, 845, 1192, 1240, 1565, 1693, 2212
BceAI ACGGC 1 cut(s) 1933
BcgI CGANNNNNNTGC 4 cut(s) 65, 99, 1630, 1664
BciT130I CCWGG 5 cut(s) 2283, 2513, 2699, 2947, 2990
BciVI GTATCC 1 cut(s) 1745
BclI TGATCA 3 cut(s) 1189, 1255, 2713
BcnI CCSGG 1 cut(s) 277
BcoDI GTCTC 5 cut(s) 115, 1050, 1656, 1956, 2865
BfaI CTAG 8 cut(s) 9, 900, 1110, 1278, 1746, 1779, 1851, 2129
BfmI CTRYAG 1 cut(s) 2190
BfuAI ACCTGC 1 cut(s) 255
BfuI GTATCC 1 cut(s) 1745
BglII AGATCT 1 cut(s) 1274
BisI GCNGC 6 cut(s) 38, 262, 730, 953, 1518, 2193
BlnI CCTAGG 1 cut(s) 8
BlsI GCNGC 6 cut(s) 39, 263, 731, 954, 1519, 2194
BmcAI AGTACT 2 cut(s) 2476, 2499
Bme1390I CCNGG 6 cut(s) 277, 2283, 2513, 2699, 2947, 2990
Bme18I GGWCC 1 cut(s) 2986
BmeRI GACNNNNNGTC 1 cut(s) 570
BmeT110I CYCGRG 1 cut(s) 1053
BmgBI CACGTC 1 cut(s) 425
BmgT120I GGNCC 3 cut(s) 1867, 1888, 2986
BmiI GGNNCC 5 cut(s) 85, 384, 490, 613, 2508
BmrFI CCNGG 6 cut(s) 277, 2283, 2513, 2699, 2947, 2990
BmrI ACTGGG 1 cut(s) 2763
BmsI GCATC 7 cut(s) 404, 1197, 1219, 1430, 1960, 2149, 2343
BmtI GCTAGC 1 cut(s) 903
BmuI ACTGGG 1 cut(s) 2763
BpmI CTGGAG 3 cut(s) 2553, 2666, 2974
Bpu10I CCTNAGC 1 cut(s) 2199
Bpu14I TTCGAA 1 cut(s) 75
BpuEI CTTGAG 3 cut(s) 675, 1135, 1938
BpuMI CCSGG 1 cut(s) 277
BsaBI GATNNNNATC 4 cut(s) 76, 939, 2124, 2579
BsaHI GRCGYC 1 cut(s) 255
BsaI GGTCTC 2 cut(s) 115, 1050
BsaJI CCNNGG 8 cut(s) 8, 673, 2019, 2281, 2511, 2523, 2698, 2803
BsaWI WCCGGW 2 cut(s) 80, 379
BsaXI ACNNNNNCTCC 2 cut(s) 2862, 2892
Bsc4I CCNNNNNNNGG 6 cut(s) 674, 912, 1067, 1297, 2383, 2952
Bse118I RCCGGY 2 cut(s) 379, 1036
Bse1I ACTGG 5 cut(s) 800, 1915, 2570, 2758, 2957
Bse21I CCTNAGG 1 cut(s) 126
Bse8I GATNNNNATC 4 cut(s) 76, 939, 2124, 2579
BseBI CCWGG 5 cut(s) 2283, 2513, 2699, 2947, 2990
BseDI CCNNGG 8 cut(s) 8, 673, 2019, 2281, 2511, 2523, 2698, 2803
BseGI GGATG 5 cut(s) 272, 343, 1210, 1251, 2140
BseJI GATNNNNATC 4 cut(s) 76, 939, 2124, 2579
BseLI CCNNNNNNNGG 6 cut(s) 674, 912, 1067, 1297, 2383, 2952
BseMII CTCAG 6 cut(s) 117, 1308, 1998, 2190, 2208, 2555
BseNI ACTGG 5 cut(s) 800, 1915, 2570, 2758, 2957
BseXI GCAGC 5 cut(s) 49, 741, 964, 1529, 2204
BseYI CCCAGC 1 cut(s) 2318
BsgI GTGCAG 1 cut(s) 1536
Bsh1236I CGCG 1 cut(s) 727
Bsh1285I CGRYCG 1 cut(s) 1174
BshFI GGCC 7 cut(s) 708, 1036, 1869, 1889, 2346, 2566, 2993
BshNI GGYRCC 2 cut(s) 83, 382
BshTI ACCGGT 1 cut(s) 379
BsiEI CGRYCG 1 cut(s) 1174
BsiHKAI GWGCWC 2 cut(s) 1222, 2461
BsiHKCI CYCGRG 1 cut(s) 1053
BsiSI CCGG 6 cut(s) 44, 81, 276, 380, 392, 1037
BslFI GGGAC 4 cut(s) 45, 577, 2335, 2451
BslI CCNNNNNNNGG 6 cut(s) 674, 912, 1067, 1297, 2383, 2952
BsmAI GTCTC 5 cut(s) 115, 1050, 1656, 1956, 2865
BsmBI CGTCTC 1 cut(s) 1956
BsmFI GGGAC 4 cut(s) 45, 577, 2335, 2451
BsmI GAATGC 3 cut(s) 1653, 2770, 2868
BsnI GGCC 7 cut(s) 708, 1036, 1869, 1889, 2346, 2566, 2993
Bso31I GGTCTC 2 cut(s) 115, 1050
BsoBI CYCGRG 1 cut(s) 1053
Bsp119I TTCGAA 1 cut(s) 75
Bsp1286I GDGCHC 3 cut(s) 164, 1222, 2461
BspACI CCGC 6 cut(s) 164, 240, 259, 262, 349, 2915
BspANI GGCC 7 cut(s) 708, 1036, 1869, 1889, 2346, 2566, 2993
BspCNI CTCAG 6 cut(s) 118, 1309, 1997, 2191, 2209, 2554
BspFNI CGCG 1 cut(s) 727
BspLI GGNNCC 5 cut(s) 85, 384, 490, 613, 2508
BspMAI CTGCAG 1 cut(s) 2194
BspMI ACCTGC 1 cut(s) 255
BspOI GCTAGC 1 cut(s) 903
BspPI GGATC 4 cut(s) 606, 619, 883, 1843
BspQI GCTCTTC 1 cut(s) 1716
BspT104I TTCGAA 1 cut(s) 75
BspT107I GGYRCC 2 cut(s) 83, 382
BspTNI GGTCTC 2 cut(s) 115, 1050
BsrFI RCCGGY 2 cut(s) 379, 1036
BsrI ACTGG 5 cut(s) 800, 1915, 2570, 2758, 2957
BssAI RCCGGY 2 cut(s) 379, 1036
BssECI CCNNGG 8 cut(s) 8, 673, 2019, 2281, 2511, 2523, 2698, 2803
BssNAI GTATAC 1 cut(s) 1845
BssNI GRCGYC 1 cut(s) 255
BssSI CACGAG 2 cut(s) 17, 1215
BssT1I CCWWGG 4 cut(s) 8, 673, 2019, 2523
Bst1107I GTATAC 1 cut(s) 1845
Bst2BI CACGAG 2 cut(s) 17, 1215
Bst2UI CCWGG 5 cut(s) 2283, 2513, 2699, 2947, 2990
Bst4CI ACNGT 7 cut(s) 643, 682, 1267, 1599, 2479, 2804, 2876
Bst6I CTCTTC 3 cut(s) 1716, 1806, 2105
BstACI GRCGYC 1 cut(s) 255
BstAPI GCANNNNNTGC 2 cut(s) 958, 2456
BstBI TTCGAA 1 cut(s) 75
BstC8I GCNNGC 7 cut(s) 164, 227, 901, 1460, 1946, 2709, 2826
BstDEI CTNAG 8 cut(s) 126, 1317, 1984, 2199, 2217, 2541, 2657, 2923
BstDSI CCRYGG 1 cut(s) 2803
BstEII GGTNACC 2 cut(s) 279, 2024
BstF5I GGATG 5 cut(s) 272, 343, 1210, 1251, 2140
BstFNI CGCG 1 cut(s) 727
BstHHI GCGC 3 cut(s) 729, 1347, 1522
BstMAI GTCTC 5 cut(s) 115, 1050, 1656, 1956, 2865
BstMCI CGRYCG 1 cut(s) 1174
BstMWI GCNNNNNNNGC 7 cut(s) 261, 735, 952, 958, 1351, 2456, 2648
BstNI CCWGG 5 cut(s) 2283, 2513, 2699, 2947, 2990
BstNSI RCATGY 2 cut(s) 1462, 2396
BstPI GGTNACC 2 cut(s) 279, 2024
BstSCI CCNGG 6 cut(s) 275, 2281, 2511, 2697, 2945, 2988
BstSFI CTRYAG 1 cut(s) 2190
BstUI CGCG 1 cut(s) 727
BstV1I GCAGC 5 cut(s) 49, 741, 964, 1529, 2204
BstX2I RGATCY 4 cut(s) 611, 875, 1274, 1835
BstXI CCANNNNNNTGG 1 cut(s) 2036
BstYI RGATCY 4 cut(s) 611, 875, 1274, 1835
BstZ17I GTATAC 1 cut(s) 1845
Bsu36I CCTNAGG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 1745
BsuRI GGCC 7 cut(s) 708, 1036, 1869, 1889, 2346, 2566, 2993
BtgI CCRYGG 1 cut(s) 2803
BtgZI GCGATG 1 cut(s) 1488
BtrI CACGTC 1 cut(s) 425
BtsCI GGATG 5 cut(s) 272, 343, 1210, 1251, 2140
BtsIMutI CAGTG 6 cut(s) 807, 1604, 2487, 2577, 2751, 2881
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 7 cut(s) 164, 227, 901, 1460, 1946, 2709, 2826
CaiI CAGNNNCTG 3 cut(s) 2198, 2952, 2989
CfoI GCGC 3 cut(s) 729, 1347, 1522
Cfr10I RCCGGY 2 cut(s) 379, 1036
Cfr13I GGNCC 3 cut(s) 1867, 1888, 2986
CseI GACGC 2 cut(s) 244, 1464
Csp6I GTAC 7 cut(s) 134, 383, 678, 1281, 2237, 2475, 2498
CspAI ACCGGT 1 cut(s) 379
CviQI GTAC 7 cut(s) 134, 383, 678, 1281, 2237, 2475, 2498
DdeI CTNAG 8 cut(s) 126, 1317, 1984, 2199, 2217, 2541, 2657, 2923
DraIII CACNNNGTG 1 cut(s) 1204
DriI GACNNNNNGTC 1 cut(s) 570
EaeI YGGCCR 3 cut(s) 706, 2344, 2991
Eam1104I CTCTTC 3 cut(s) 1716, 1806, 2105
Eam1105I GACNNNNNGTC 1 cut(s) 570
EarI CTCTTC 3 cut(s) 1716, 1806, 2105
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 4 cut(s) 8, 673, 2019, 2523
Eco24I GRGCYC 1 cut(s) 164
Eco31I GGTCTC 2 cut(s) 115, 1050
Eco47I GGWCC 1 cut(s) 2986
Eco57I CTGAAG 2 cut(s) 749, 1601
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 1053
Eco91I GGTNACC 2 cut(s) 279, 2024
EcoO109I RGGNCCY 1 cut(s) 2986
EcoO65I GGTNACC 2 cut(s) 279, 2024
EcoRI GAATTC 1 cut(s) 926
EcoRII CCWGG 5 cut(s) 2281, 2511, 2697, 2945, 2988
EcoT14I CCWWGG 4 cut(s) 8, 673, 2019, 2523
EcoT22I ATGCAT 5 cut(s) 1024, 1212, 1464, 1632, 2398
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 4 cut(s) 8, 673, 2019, 2523
Esp3I CGTCTC 1 cut(s) 1956
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FaqI GGGAC 4 cut(s) 45, 577, 2335, 2451
FauI CCCGC 1 cut(s) 171
FauNDI CATATG 2 cut(s) 588, 957
FbaI TGATCA 3 cut(s) 1189, 1255, 2713
FblI GTMKAC 1 cut(s) 1844
Fnu4HI GCNGC 6 cut(s) 38, 262, 730, 953, 1518, 2193
FokI GGATG 5 cut(s) 259, 350, 1197, 1258, 2147
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 6 cut(s) 38, 262, 730, 953, 1518, 2193
FspBI CTAG 8 cut(s) 9, 900, 1110, 1278, 1746, 1779, 1851, 2129
GlaI GCGC 3 cut(s) 728, 1346, 1521
GluI GCNGC 6 cut(s) 38, 262, 730, 953, 1518, 2193
GsaI CCCAGC 1 cut(s) 2322
GsuI CTGGAG 3 cut(s) 2553, 2666, 2974
HaeIII GGCC 7 cut(s) 708, 1036, 1869, 1889, 2346, 2566, 2993
HapII CCGG 6 cut(s) 44, 81, 276, 380, 392, 1037
HgaI GACGC 2 cut(s) 244, 1464
HhaI GCGC 3 cut(s) 729, 1347, 1522
Hin1I GRCGYC 1 cut(s) 255
Hin6I GCGC 3 cut(s) 727, 1345, 1520
HinP1I GCGC 3 cut(s) 727, 1345, 1520
HincII GTYRAC 2 cut(s) 270, 2071
HindII GTYRAC 2 cut(s) 270, 2071
HindIII AAGCTT 2 cut(s) 1667, 2532
HpaII CCGG 6 cut(s) 44, 81, 276, 380, 392, 1037
Hpy166II GTNNAC 5 cut(s) 270, 557, 1845, 2071, 2140
Hpy8I GTNNAC 5 cut(s) 270, 557, 1845, 2071, 2140
HpyAV CCTTC 8 cut(s) 815, 972, 1470, 2257, 2353, 2539, 2617, 2968
HpyCH4III ACNGT 7 cut(s) 643, 682, 1267, 1599, 2479, 2804, 2876
HpyCH4IV ACGT 3 cut(s) 58, 424, 2831
HpyF10VI GCNNNNNNNGC 7 cut(s) 261, 735, 952, 958, 1351, 2456, 2648
HpyF3I CTNAG 8 cut(s) 126, 1317, 1984, 2199, 2217, 2541, 2657, 2923
HpySE526I ACGT 3 cut(s) 58, 424, 2831
Hsp92I GRCGYC 1 cut(s) 255
HspAI GCGC 3 cut(s) 727, 1345, 1520
KpnI GGTACC 1 cut(s) 386
Ksp22I TGATCA 3 cut(s) 1189, 1255, 2713
LguI GCTCTTC 1 cut(s) 1716
LmnI GCTCC 8 cut(s) 494, 650, 695, 908, 1293, 2200, 2884, 3004
Lsp1109I GCAGC 5 cut(s) 49, 741, 964, 1529, 2204
LweI GCATC 7 cut(s) 404, 1197, 1219, 1430, 1960, 2149, 2343
MaeI CTAG 8 cut(s) 9, 900, 1110, 1278, 1746, 1779, 1851, 2129
MaeII ACGT 3 cut(s) 58, 424, 2831
MaeIII GTNAC 7 cut(s) 279, 315, 635, 802, 2024, 2058, 2386
MfeI CAATTG 1 cut(s) 2231
MflI RGATCY 4 cut(s) 611, 875, 1274, 1835
MhlI GDGCHC 3 cut(s) 164, 1222, 2461
MlsI TGGCCA 2 cut(s) 708, 2993
MluNI TGGCCA 2 cut(s) 708, 2993
MlyI GAGTC 1 cut(s) 1952
MmeI TCCRAC 1 cut(s) 2859
Mox20I TGGCCA 2 cut(s) 708, 2993
Mph1103I ATGCAT 5 cut(s) 1024, 1212, 1464, 1632, 2398
MroXI GAANNNNTTC 3 cut(s) 762, 926, 2363
MscI TGGCCA 2 cut(s) 708, 2993
MseI TTAA 9 cut(s) 413, 999, 1017, 1716, 2045, 2270, 2672, 2940, 3006
MslI CAYNNNNRTG 5 cut(s) 180, 234, 423, 1512, 2145
Msp20I TGGCCA 2 cut(s) 708, 2993
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 6 cut(s) 44, 81, 276, 380, 392, 1037
MspR9I CCNGG 6 cut(s) 277, 2283, 2513, 2699, 2947, 2990
MunI CAATTG 1 cut(s) 2231
Mva1269I GAATGC 3 cut(s) 1653, 2770, 2868
MvaI CCWGG 5 cut(s) 2283, 2513, 2699, 2947, 2990
MvnI CGCG 1 cut(s) 727
MwoI GCNNNNNNNGC 7 cut(s) 261, 735, 952, 958, 1351, 2456, 2648
NciI CCSGG 1 cut(s) 277
NdeI CATATG 2 cut(s) 588, 957
NheI GCTAGC 1 cut(s) 899
NlaIV GGNNCC 5 cut(s) 85, 384, 490, 613, 2508
NmuCI GTSAC 6 cut(s) 279, 315, 635, 802, 2024, 2058
NsiI ATGCAT 5 cut(s) 1024, 1212, 1464, 1632, 2398
NspI RCATGY 2 cut(s) 1462, 2396
NspV TTCGAA 1 cut(s) 75
OliI CACNNNNGTG 2 cut(s) 423, 2145
PaeI GCATGC 1 cut(s) 1462
PaeR7I CTCGAG 1 cut(s) 1053
PciSI GCTCTTC 1 cut(s) 1716
PctI GAATGC 3 cut(s) 1653, 2770, 2868
PdmI GAANNNNTTC 3 cut(s) 762, 926, 2363
PflFI GACNNNGTC 1 cut(s) 1599
PflMI CCANNNNNTGG 3 cut(s) 674, 2383, 2952
PinAI ACCGGT 1 cut(s) 379
PkrI GCNGC 6 cut(s) 39, 263, 731, 954, 1519, 2194
Ple19I CGATCG 1 cut(s) 1174
PleI GAGTC 1 cut(s) 1952
PpsI GAGTC 1 cut(s) 1952
PpuMI RGGWCCY 1 cut(s) 2986
PshBI ATTAAT 1 cut(s) 1017
PsiI TTATAA 2 cut(s) 686, 1998
Psp5II RGGWCCY 1 cut(s) 2986
Psp6I CCWGG 5 cut(s) 2281, 2511, 2697, 2945, 2988
PspEI GGTNACC 2 cut(s) 279, 2024
PspFI CCCAGC 1 cut(s) 2318
PspGI CCWGG 5 cut(s) 2281, 2511, 2697, 2945, 2988
PspN4I GGNNCC 5 cut(s) 85, 384, 490, 613, 2508
PspPI GGNCC 3 cut(s) 1867, 1888, 2986
PspPPI RGGWCCY 1 cut(s) 2986
PsrI GAACNNNNNNTAC 2 cut(s) 2490, 2522
PstI CTGCAG 1 cut(s) 2194
PstNI CAGNNNCTG 3 cut(s) 2198, 2952, 2989
PsuI RGATCY 4 cut(s) 611, 875, 1274, 1835
PsyI GACNNNGTC 1 cut(s) 1599
PvuI CGATCG 1 cut(s) 1174
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 7 cut(s) 135, 384, 679, 1282, 2238, 2476, 2499
RsaNI GTAC 7 cut(s) 134, 383, 678, 1281, 2237, 2475, 2498
RseI CAYNNNNRTG 5 cut(s) 180, 234, 423, 1512, 2145
SapI GCTCTTC 1 cut(s) 1716
SaqAI TTAA 9 cut(s) 413, 999, 1017, 1716, 2045, 2270, 2672, 2940, 3006
SatI GCNGC 6 cut(s) 38, 262, 730, 953, 1518, 2193
Sau96I GGNCC 3 cut(s) 1867, 1888, 2986
ScaI AGTACT 2 cut(s) 2476, 2499
SchI GAGTC 1 cut(s) 1952
ScrFI CCNGG 6 cut(s) 277, 2283, 2513, 2699, 2947, 2990
SduI GDGCHC 3 cut(s) 164, 1222, 2461
SfaNI GCATC 7 cut(s) 404, 1197, 1219, 1430, 1960, 2149, 2343
SfcI CTRYAG 1 cut(s) 2190
Sfr274I CTCGAG 1 cut(s) 1053
SfuI TTCGAA 1 cut(s) 75
SinI GGWCC 1 cut(s) 2986
SlaI CTCGAG 1 cut(s) 1053
SmiMI CAYNNNNRTG 5 cut(s) 180, 234, 423, 1512, 2145
SmlI CTYRAG 4 cut(s) 690, 1053, 1150, 1953
SmoI CTYRAG 4 cut(s) 690, 1053, 1150, 1953
SphI GCATGC 1 cut(s) 1462
SsiI CCGC 6 cut(s) 164, 240, 259, 262, 349, 2915
SspI AATATT 2 cut(s) 145, 2590
SspMI CTAG 8 cut(s) 9, 900, 1110, 1278, 1746, 1779, 1851, 2129
StyD4I CCNGG 6 cut(s) 275, 2281, 2511, 2697, 2945, 2988
StyI CCWWGG 4 cut(s) 8, 673, 2019, 2523
TaaI ACNGT 7 cut(s) 643, 682, 1267, 1599, 2479, 2804, 2876
TaiI ACGT 3 cut(s) 61, 427, 2834
TaqI TCGA 3 cut(s) 75, 1054, 2357
TatI WGTACW 2 cut(s) 2474, 2497
TauI GCSGC 1 cut(s) 264
Tru1I TTAA 9 cut(s) 413, 999, 1017, 1716, 2045, 2270, 2672, 2940, 3006
Tru9I TTAA 9 cut(s) 413, 999, 1017, 1716, 2045, 2270, 2672, 2940, 3006
TscAI CASTG 6 cut(s) 807, 1604, 2494, 2577, 2758, 2881
TseFI GTSAC 6 cut(s) 279, 315, 635, 802, 2024, 2058
TseI GCWGC 5 cut(s) 37, 729, 952, 1517, 2192
Tsp45I GTSAC 6 cut(s) 279, 315, 635, 802, 2024, 2058
TspGWI ACGGA 1 cut(s) 2856
TspRI CASTG 6 cut(s) 807, 1604, 2494, 2577, 2758, 2881
Tth111I GACNNNGTC 1 cut(s) 1599
Van91I CCANNNNNTGG 3 cut(s) 674, 2383, 2952
VpaK11BI GGWCC 1 cut(s) 2986
VspI ATTAAT 1 cut(s) 1017
XapI RAATTY 5 cut(s) 926, 974, 1333, 1482, 1925
XbaI TCTAGA 3 cut(s) 1778, 1850, 2128
XceI RCATGY 2 cut(s) 1462, 2396
XhoI CTCGAG 1 cut(s) 1053
XmaJI CCTAGG 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 1844
XmnI GAANNNNTTC 3 cut(s) 762, 926, 2363
XspI CTAG 8 cut(s) 9, 900, 1110, 1278, 1746, 1779, 1851, 2129
ZrmI AGTACT 2 cut(s) 2476, 2499
Zsp2I ATGCAT 5 cut(s) 1024, 1212, 1464, 1632, 2398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.