Rmu_sc0000383.1_g000009

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000383.1
OG0009048
Physical Location & Seq
Reverse (-)
42049 .. 43634
1586 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
atggccttcgtttcctccgctgggaggctccttttcgcctccgtcttccttctctccgcttggcaagagttcaatgaatttggttcggatgggggacctgcagctaagtatttgagaccaaagctcgatgttttctcgagccatctgttagctcataccggtgtgcaagtgccgaagcaagttgagcagattaagcatttagtggcggcggcgattgtcttgaagggtgtcgggggtgttcttttcatctttggaagtactctcggagctgttctgctgcttctgcatcaggccattgcaactcctatattgtatgacttctacaactatgatgcggagaaaaaagaattcaatcaacttttcctcaagtttacccagaatttggcattgtttggggctctgctctttttcataagcatgaagatatcaatgccaaagagactcaggaaaagagctcccaaagctaaaactacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

158

Amino Acids

17.48

Weight (kDa)

9.94

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 140 4.5e-59 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 106
AccB7I CCANNNNNTGG 1 cut(s) 382
AciI CCGC 5 cut(s) 18, 57, 206, 209, 335
AcsI RAATTY 3 cut(s) 77, 347, 379
AfaI GTAC 1 cut(s) 259
AfiI CCNNNNNNNGG 3 cut(s) 21, 24, 382
AgeI ACCGGT 1 cut(s) 158
AgsI TTSAA 3 cut(s) 73, 223, 352
AluBI AGCT 6 cut(s) 104, 124, 152, 269, 455, 464
AluI AGCT 6 cut(s) 104, 124, 152, 269, 455, 464
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 2 cut(s) 109, 433
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 2 cut(s) 3, 291
ApeKI GCWGC 2 cut(s) 101, 277
ApoI RAATTY 3 cut(s) 77, 347, 379
AsiGI ACCGGT 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 1 cut(s) 95
BanII GRGCYC 2 cut(s) 400, 457
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 2 cut(s) 113, 264
BccI CCATC 2 cut(s) 83, 150
BcoDI GTCTC 2 cut(s) 109, 433
BfmI CTRYAG 1 cut(s) 99
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 4 cut(s) 102, 207, 210, 278
BlsI GCNGC 4 cut(s) 103, 208, 211, 279
BmcAI AGTACT 1 cut(s) 259
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 2 cut(s) 29, 96
BmsI GCATC 2 cut(s) 295, 322
BpiI GAAGAC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 109
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 24, 382
Bse118I RCCGGY 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 294
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 3 cut(s) 21, 24, 382
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 457
BseXI GCAGC 2 cut(s) 113, 264
BseYI CCCAGC 1 cut(s) 20
BshFI GGCC 2 cut(s) 5, 293
BshTI ACCGGT 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 108
BslI CCNNNNNNNGG 3 cut(s) 21, 24, 382
BsmAI GTCTC 2 cut(s) 109, 433
BsmFI GGGAC 1 cut(s) 108
BsnI GGCC 2 cut(s) 5, 293
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 400, 457
BspACI CCGC 5 cut(s) 18, 57, 206, 209, 335
BspANI GGCC 2 cut(s) 5, 293
BspCNI CTCAG 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 29, 96
BspMAI CTGCAG 1 cut(s) 103
BspMI ACCTGC 1 cut(s) 106
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BstDEI CTNAG 2 cut(s) 105, 443
BstF5I GGATG 1 cut(s) 94
BstMAI GTCTC 2 cut(s) 109, 433
BstMWI GCNNNNNNNGC 4 cut(s) 184, 193, 283, 461
BstSFI CTRYAG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 113, 264
BstV2I GAAGAC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 5, 293
BtsCI GGATG 1 cut(s) 94
BveI ACCTGC 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 258
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 2 cut(s) 418, 474
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 2 cut(s) 105, 443
Ecl136II GAGCTC 1 cut(s) 455
Eco24I GRGCYC 2 cut(s) 400, 457
Eco31I GGTCTC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 426
Eco47I GGWCC 1 cut(s) 95
Eco53kI GAGCTC 1 cut(s) 455
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 347
EcoRV GATATC 1 cut(s) 426
EcoT38I GRGCYC 2 cut(s) 400, 457
FaeI CATG 2 cut(s) 421, 477
FaiI YATR 7 cut(s) 156, 308, 315, 330, 413, 419, 475
FaqI GGGAC 1 cut(s) 108
FatI CATG 2 cut(s) 417, 473
Fnu4HI GCNGC 4 cut(s) 102, 207, 210, 278
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 2 cut(s) 400, 457
Fsp4HI GCNGC 4 cut(s) 102, 207, 210, 278
GluI GCNGC 4 cut(s) 102, 207, 210, 278
GsaI CCCAGC 1 cut(s) 24
HaeIII GGCC 2 cut(s) 5, 293
HapII CCGG 1 cut(s) 159
Hin1II CATG 2 cut(s) 421, 477
HinfI GANTC 1 cut(s) 441
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 372
Hpy188I TCNGA 2 cut(s) 88, 266
Hpy188III TCNNGA 3 cut(s) 136, 220, 445
Hpy8I GTNNAC 1 cut(s) 372
HpyAV CCTTC 3 cut(s) 16, 59, 217
HpyCH4V TGCA 4 cut(s) 101, 166, 286, 299
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 193, 283, 461
HpyF3I CTNAG 2 cut(s) 105, 443
Hsp92II CATG 2 cut(s) 421, 477
LmnI GCTCC 3 cut(s) 33, 266, 460
LpnPI CCDG 6 cut(s) 6, 111, 172, 275, 389, 430
Lsp1109I GCAGC 2 cut(s) 113, 264
LweI GCATC 2 cut(s) 295, 322
MboII GAAGA 2 cut(s) 37, 433
MhlI GDGCHC 2 cut(s) 400, 457
MluCI AATT 3 cut(s) 77, 347, 379
MlyI GAGTC 1 cut(s) 435
MnlI CCTC 4 cut(s) 18, 25, 49, 374
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 2 cut(s) 159, 416
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 1 cut(s) 159
MwoI GCNNNNNNNGC 4 cut(s) 184, 193, 283, 461
NlaIII CATG 2 cut(s) 421, 477
NlaIV GGNNCC 2 cut(s) 29, 96
PaeR7I CTCGAG 1 cut(s) 136
PflMI CCANNNNNTGG 1 cut(s) 382
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 4 cut(s) 103, 208, 211, 279
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
PpuMI RGGWCCY 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 457
Psp5II RGGWCCY 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 20
PspN4I GGNNCC 2 cut(s) 29, 96
PspPI GGNCC 1 cut(s) 95
PspPPI RGGWCCY 1 cut(s) 95
PstI CTGCAG 1 cut(s) 103
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
RseI CAYNNNNRTG 2 cut(s) 159, 416
SacI GAGCTC 1 cut(s) 457
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 4 cut(s) 102, 207, 210, 278
Sau96I GGNCC 1 cut(s) 95
ScaI AGTACT 1 cut(s) 259
SchI GAGTC 1 cut(s) 435
SduI GDGCHC 2 cut(s) 400, 457
SetI ASST 7 cut(s) 100, 106, 126, 154, 271, 457, 466
SfaNI GCATC 2 cut(s) 295, 322
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 136
SinI GGWCC 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 136
SmiMI CAYNNNNRTG 2 cut(s) 159, 416
SmlI CTYRAG 2 cut(s) 136, 365
SmoI CTYRAG 2 cut(s) 136, 365
Sse9I AATT 3 cut(s) 77, 347, 379
SsiI CCGC 5 cut(s) 18, 57, 206, 209, 335
SstI GAGCTC 1 cut(s) 457
TaqI TCGA 2 cut(s) 126, 137
TasI AATT 3 cut(s) 77, 347, 379
TatI WGTACW 1 cut(s) 257
TauI GCSGC 2 cut(s) 209, 212
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 2 cut(s) 101, 277
TspDTI ATGAA 4 cut(s) 90, 235, 400, 434
TspGWI ACGGA 1 cut(s) 31
Van91I CCANNNNNTGG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 3 cut(s) 77, 347, 379
XhoI CTCGAG 1 cut(s) 136
ZrmI AGTACT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.