Rmu_sc0000393.1_g000004

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000393.1
OG0003921
Physical Location & Seq
Reverse (-)
12758 .. 13392
635 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
atggaaactacaaagagttctggtacagagaaaatagacaccaggaccagtactttagatgaatttgaagatggaagccaactgattgtggtgaactgctaccctgcatgccctgaacctgatttaaccctaggcattccacctcattctgattatggcatcctcactcttctccttcaagaccgagtccagggtcttcaaatacagcaccaaggaaaatgggtgaccgtggaaccacttccgaattcattcatcgtcaacgttggtgatcatcttgagatatttagcaatgggagatacaaaagcgtgctgcatagggttgttgtcaactcatccaagtctcgaatctctgttgcttcattgcacagcctgccattcgacaccgttgttcgcccttcacctaaactcgtcgacgaagccaaccctgttcgttacagggacacagactttgccaattttctcgagtatattgcttcgcgtgaccaccagacgaacagtaagagtttcttgcaatcgaggaaattgacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

176

Amino Acids

19.77

Weight (kDa)

6.05

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 27 - 121 3.2e-36 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 411
AccII CGCG 1 cut(s) 478
AclI AACGTT 1 cut(s) 261
AcsI RAATTY 2 cut(s) 62, 244
AfaI GTAC 2 cut(s) 25, 52
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 3 cut(s) 68, 179, 200
AjnI CCWGG 2 cut(s) 41, 189
Alw26I GTCTC 1 cut(s) 345
Ama87I CYCGRG 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 2 cut(s) 62, 244
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
Asp700I GAANNNNTTC 2 cut(s) 237, 248
AspA2I CCTAGG 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 45
AsuHPI GGTGA 4 cut(s) 103, 235, 278, 390
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 1 cut(s) 45
AvrII CCTAGG 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 1 cut(s) 297
BccI CCATC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 2 cut(s) 43, 191
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 1 cut(s) 131
BisI GCNGC 1 cut(s) 311
BlnI CCTAGG 1 cut(s) 130
BlsI GCNGC 1 cut(s) 312
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 2 cut(s) 43, 191
Bme18I GGWCC 1 cut(s) 45
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 2 cut(s) 43, 191
BmsI GCATC 1 cut(s) 168
BpiI GAAGAC 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 296
BsaJI CCNNGG 4 cut(s) 130, 190, 211, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 48
Bse3DI GCAATG 2 cut(s) 295, 359
BseBI CCWGG 2 cut(s) 43, 191
BseDI CCNNGG 4 cut(s) 130, 190, 211, 228
BseGI GGATG 2 cut(s) 159, 332
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMI GCAATG 2 cut(s) 295, 359
BseNI ACTGG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 478
BsiHKCI CYCGRG 1 cut(s) 461
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 190
BsmAI GTCTC 1 cut(s) 345
BsmFI GGGAC 1 cut(s) 452
BsmI GAATGC 1 cut(s) 135
BsoBI CYCGRG 1 cut(s) 461
Bsp143I GATC 1 cut(s) 268
BspFNI CGCG 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 234
BsrDI GCAATG 2 cut(s) 295, 359
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 4 cut(s) 130, 190, 211, 228
BssMI GATC 1 cut(s) 268
BssT1I CCWWGG 2 cut(s) 130, 211
Bst2UI CCWGG 2 cut(s) 43, 191
Bst4CI ACNGT 3 cut(s) 229, 385, 497
Bst6I CTCTTC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 370
BstC8I GCNNGC 3 cut(s) 109, 308, 371
BstDSI CCRYGG 1 cut(s) 228
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 2 cut(s) 159, 332
BstFNI CGCG 1 cut(s) 478
BstKTI GATC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 1 cut(s) 268
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNI CCWGG 2 cut(s) 43, 191
BstNSI RCATGY 1 cut(s) 111
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 2 cut(s) 41, 189
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 1 cut(s) 297
BstV2I GAAGAC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 2 cut(s) 159, 332
Cac8I GCNNGC 3 cut(s) 109, 308, 371
Cfr13I GGNCC 1 cut(s) 45
Csp6I GTAC 2 cut(s) 24, 51
CviAII CATG 1 cut(s) 108
CviJI RGCY 3 cut(s) 78, 369, 419
CviKI_1 RGCY 3 cut(s) 78, 369, 419
CviQI GTAC 2 cut(s) 24, 51
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 130, 211
Eco47I GGWCC 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 461
Eco91I GGTNACC 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 41, 189
EcoT14I CCWWGG 2 cut(s) 130, 211
ErhI CCWWGG 2 cut(s) 130, 211
FaeI CATG 1 cut(s) 111
FaiI YATR 4 cut(s) 109, 156, 315, 468
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 1 cut(s) 452
FatI CATG 1 cut(s) 107
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 2 cut(s) 146, 319
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 1 cut(s) 131
GluI GCNGC 1 cut(s) 311
Hin1II CATG 1 cut(s) 111
HincII GTYRAC 3 cut(s) 259, 328, 412
HindII GTYRAC 3 cut(s) 259, 328, 412
HinfI GANTC 2 cut(s) 186, 345
HphI GGTGA 4 cut(s) 103, 235, 278, 390
Hpy166II GTNNAC 4 cut(s) 94, 259, 328, 412
Hpy188I TCNGA 2 cut(s) 151, 243
Hpy188III TCNNGA 4 cut(s) 179, 275, 342, 461
Hpy8I GTNNAC 4 cut(s) 94, 259, 328, 412
Hpy99I CGWCG 2 cut(s) 413, 416
HpyAV CCTTC 2 cut(s) 185, 405
HpyCH4III ACNGT 3 cut(s) 229, 385, 497
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 107, 313, 364, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 1 cut(s) 268
Lsp1109I GCAGC 1 cut(s) 297
LweI GCATC 1 cut(s) 168
MaeI CTAG 1 cut(s) 131
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 223, 431, 479
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MboII GAAGA 3 cut(s) 80, 161, 188
MluCI AATT 4 cut(s) 62, 244, 454, 521
MlyI GAGTC 1 cut(s) 195
MnlI CCTC 3 cut(s) 153, 173, 510
MroXI GAANNNNTTC 2 cut(s) 237, 248
MseI TTAA 1 cut(s) 125
MspR9I CCNGG 2 cut(s) 43, 191
Mva1269I GAATGC 1 cut(s) 135
MvaI CCWGG 2 cut(s) 43, 191
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 1 cut(s) 370
NdeII GATC 1 cut(s) 268
NlaIII CATG 1 cut(s) 111
NlaIV GGNNCC 1 cut(s) 234
NmuCI GTSAC 2 cut(s) 223, 479
NspI RCATGY 1 cut(s) 111
PaeI GCATGC 1 cut(s) 111
PaeR7I CTCGAG 1 cut(s) 461
PctI GAATGC 1 cut(s) 135
PdmI GAANNNNTTC 2 cut(s) 237, 248
PfeI GAWTC 1 cut(s) 345
PflFI GACNNNGTC 1 cut(s) 185
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
Psp1406I AACGTT 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 41, 189
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 2 cut(s) 41, 189
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 45
PsyI GACNNNGTC 1 cut(s) 185
RsaI GTAC 2 cut(s) 25, 52
RsaNI GTAC 2 cut(s) 24, 51
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 1 cut(s) 45
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 2 cut(s) 43, 191
SetI ASST 4 cut(s) 121, 145, 264, 403
SfaNI GCATC 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 461
SgrDI CGTCGACG 1 cut(s) 410
SinI GGWCC 1 cut(s) 45
SlaI CTCGAG 1 cut(s) 461
SmlI CTYRAG 2 cut(s) 275, 461
SmoI CTYRAG 2 cut(s) 275, 461
SphI GCATGC 1 cut(s) 111
Sse9I AATT 4 cut(s) 62, 244, 454, 521
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 41, 189
StyI CCWWGG 2 cut(s) 130, 211
TaaI ACNGT 3 cut(s) 229, 385, 497
TaiI ACGT 1 cut(s) 264
TaqI TCGA 5 cut(s) 343, 378, 411, 462, 515
TaqII GACCGA 1 cut(s) 198
TasI AATT 4 cut(s) 62, 244, 454, 521
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 1 cut(s) 345
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TseFI GTSAC 2 cut(s) 223, 479
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 2 cut(s) 223, 479
TspDTI ATGAA 4 cut(s) 75, 237, 241, 348
Tth111I GACNNNGTC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 45
XapI RAATTY 2 cut(s) 62, 244
XceI RCATGY 1 cut(s) 111
XhoI CTCGAG 1 cut(s) 461
XmaJI CCTAGG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 411
XmnI GAANNNNTTC 2 cut(s) 237, 248
XspI CTAG 1 cut(s) 131
ZrmI AGTACT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.