Rmu_sc0000429.1_g000082

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000429.1
OG0012676
Physical Location & Seq
Forward (+)
397130 .. 400376
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000429.1_g000082.1.cds

Sequence Viewer

Length: 861 bp
atggacaccatcgccaaccaacactttgctgatggctggaaaacaagaaggaagtcgaggtatttccccttgctaagaaacatcgaaaacctaggtggtggggaggaagttgccatcgtgttcgaggacaaaagagtcacttatcctactaggaacgcacatgggctaatttatgaagcgggattaggttgctttgaaacactgtactgctacacaacattttccagaggtggtcgatgttcggcgtcgactctagactacgagtttttgattcaacatgtaggcaaatcaaagccgacagctgcggtggtaccagtgattggttctaggaaggataatgtgcagtttgaatattctcaacttgccaaactgattccatctgatggtggggaattagttaagaagaggaagggaatactgaaaggtcgaaagaacaaaatcaaggtgattgagaacgggcctaaaccaaaaaagaagaagtttctcaagaagctcaagctaattgatgaggtagaggtggaagaggctatgggtaatccgcaacaacttcagatccgccacgtcagcccctccggcctcctcgcattccccgccggcagctccatctccgtcatcgacacccgctcaatgaagctcgtcgtctccctccccatgcctcccccctccccctcccaatcctccttcgccaccgtcacgtcatcactatcccctttcgtcacctccgtcctgtggaccccactccctctcggccacgacctcctctccactgagccttccagctcccatctcctcgccgccgccgaccggcagggcggcatcaccctccttgacctccgcctcaagtcaccggtcctatattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

31.46

Weight (kDa)

9.63

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
Acc65I GGTACC 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 310
AccB7I CCANNNNNTGG 2 cut(s) 320, 383
AccBSI CCGCTC 1 cut(s) 624
AccI GTMKAC 1 cut(s) 248
AclWI GGATC 1 cut(s) 547
AcoI YGGCCR 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 533
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 2 cut(s) 206, 312
AfiI CCNNNNNNNGG 4 cut(s) 320, 383, 729, 804
AflIII ACRYGT 1 cut(s) 277
AgeI ACCGGT 1 cut(s) 847
AgsI TTSAA 3 cut(s) 197, 275, 350
AjiI CACGTC 2 cut(s) 562, 696
AluBI AGCT 6 cut(s) 302, 493, 499, 600, 634, 780
AluI AGCT 6 cut(s) 302, 493, 499, 600, 634, 780
Alw26I GTCTC 1 cut(s) 646
AlwI GGATC 1 cut(s) 547
AoxI GGCC 3 cut(s) 458, 574, 748
ApeKI GCWGC 2 cut(s) 302, 597
AsiGI ACCGGT 1 cut(s) 847
Asp718I GGTACC 1 cut(s) 310
AspA2I CCTAGG 1 cut(s) 91
AspS9I GGNCC 3 cut(s) 458, 732, 850
AsuHPI GGTGA 4 cut(s) 457, 709, 811, 837
AvaII GGWCC 2 cut(s) 732, 850
AvrII CCTAGG 1 cut(s) 91
BanI GGYRCC 1 cut(s) 310
BbvI GCAGC 2 cut(s) 289, 609
BccI CCATC 7 cut(s) 17, 26, 122, 377, 385, 611, 792
BcoDI GTCTC 1 cut(s) 646
BfaI CTAG 4 cut(s) 92, 150, 254, 327
BglI GCCNNNNNGGC 1 cut(s) 573
BisI GCNGC 5 cut(s) 303, 598, 795, 798, 814
BlnI CCTAGG 1 cut(s) 91
BlsI GCNGC 5 cut(s) 304, 599, 796, 799, 815
Bme18I GGWCC 2 cut(s) 732, 850
BmgBI CACGTC 2 cut(s) 562, 696
BmgT120I GGNCC 3 cut(s) 458, 732, 850
BmiI GGNNCC 2 cut(s) 312, 734
BmsI GCATC 1 cut(s) 825
BpuEI CTTGAG 3 cut(s) 470, 479, 824
BsaHI GRCGYC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 91
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bsc4I CCNNNNNNNGG 4 cut(s) 320, 383, 729, 804
Bse118I RCCGGY 3 cut(s) 593, 804, 847
Bse1I ACTGG 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 91
BseLI CCNNNNNNNGG 4 cut(s) 320, 383, 729, 804
BseMII CTCAG 1 cut(s) 759
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 3 cut(s) 569, 749, 779
BseXI GCAGC 2 cut(s) 289, 609
BsgI GTGCAG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 805
BshFI GGCC 3 cut(s) 460, 576, 750
BshNI GGYRCC 1 cut(s) 310
BshTI ACCGGT 1 cut(s) 847
BsiEI CGRYCG 1 cut(s) 805
BsiSI CCGG 4 cut(s) 573, 594, 805, 848
BslI CCNNNNNNNGG 4 cut(s) 320, 383, 729, 804
BsmAI GTCTC 1 cut(s) 646
BsmBI CGTCTC 1 cut(s) 646
BsmI GAATGC 1 cut(s) 584
BsnI GGCC 3 cut(s) 460, 576, 750
Bsp143I GATC 1 cut(s) 552
BspANI GGCC 3 cut(s) 460, 576, 750
BspCNI CTCAG 1 cut(s) 760
BspLI GGNNCC 2 cut(s) 312, 734
BspPI GGATC 1 cut(s) 547
BspT107I GGYRCC 1 cut(s) 310
BsrBI CCGCTC 1 cut(s) 624
BsrFI RCCGGY 3 cut(s) 593, 804, 847
BsrI ACTGG 1 cut(s) 314
BssAI RCCGGY 3 cut(s) 593, 804, 847
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 1 cut(s) 552
BssNI GRCGYC 1 cut(s) 245
BssT1I CCWWGG 1 cut(s) 91
Bst4CI ACNGT 2 cut(s) 204, 691
Bst6I CTCTTC 2 cut(s) 398, 516
BstACI GRCGYC 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 595
BstDEI CTNAG 2 cut(s) 74, 768
BstKTI GATC 1 cut(s) 555
BstMAI GTCTC 1 cut(s) 646
BstMBI GATC 1 cut(s) 552
BstMCI CGRYCG 1 cut(s) 805
BstMWI GCNNNNNNNGC 3 cut(s) 564, 573, 590
BstNSI RCATGY 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 289, 609
BstX2I RGATCY 1 cut(s) 552
BstYI RGATCY 1 cut(s) 552
BsuRI GGCC 3 cut(s) 460, 576, 750
BtrI CACGTC 2 cut(s) 562, 696
BtsIMutI CAGTG 3 cut(s) 200, 321, 765
Cac8I GCNNGC 1 cut(s) 595
Cfr10I RCCGGY 3 cut(s) 593, 804, 847
Cfr13I GGNCC 3 cut(s) 458, 732, 850
CseI GACGC 1 cut(s) 234
Csp6I GTAC 2 cut(s) 205, 311
CspAI ACCGGT 1 cut(s) 847
CviAII CATG 3 cut(s) 161, 278, 652
CviQI GTAC 2 cut(s) 205, 311
DdeI CTNAG 2 cut(s) 74, 768
DpnI GATC 1 cut(s) 554
DpnII GATC 1 cut(s) 552
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 2 cut(s) 398, 516
EarI CTCTTC 2 cut(s) 398, 516
EciI GGCGGA 2 cut(s) 545, 824
Eco130I CCWWGG 1 cut(s) 91
Eco47I GGWCC 2 cut(s) 732, 850
Eco57I CTGAAG 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
Esp3I CGTCTC 1 cut(s) 646
FaeI CATG 3 cut(s) 164, 281, 655
FaiI YATR 6 cut(s) 162, 174, 279, 530, 653, 856
FalI AAGNNNNNCTT 2 cut(s) 124, 156
FatI CATG 3 cut(s) 160, 277, 651
FauI CCCGC 3 cut(s) 172, 598, 629
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 5 cut(s) 303, 598, 795, 798, 814
Fsp4HI GCNGC 5 cut(s) 303, 598, 795, 798, 814
FspBI CTAG 4 cut(s) 92, 150, 254, 327
GluI GCNGC 5 cut(s) 303, 598, 795, 798, 814
HaeIII GGCC 3 cut(s) 460, 576, 750
HapII CCGG 4 cut(s) 573, 594, 805, 848
HgaI GACGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 3 cut(s) 164, 281, 655
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HinfI GANTC 4 cut(s) 135, 250, 271, 373
HpaII CCGG 4 cut(s) 573, 594, 805, 848
HphI GGTGA 4 cut(s) 457, 709, 811, 837
Hpy166II GTNNAC 2 cut(s) 249, 732
Hpy188I TCNGA 2 cut(s) 382, 552
Hpy188III TCNNGA 3 cut(s) 225, 254, 487
Hpy8I GTNNAC 2 cut(s) 249, 732
Hpy99I CGWCG 2 cut(s) 250, 641
HpyAV CCTTC 5 cut(s) 42, 325, 403, 691, 783
HpyCH4III ACNGT 2 cut(s) 204, 691
HpyCH4IV ACGT 2 cut(s) 561, 695
HpyCH4V TGCA 1 cut(s) 343
HpyF10VI GCNNNNNNNGC 3 cut(s) 564, 573, 590
HpyF3I CTNAG 2 cut(s) 74, 768
HpySE526I ACGT 2 cut(s) 561, 695
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 3 cut(s) 164, 281, 655
KpnI GGTACC 1 cut(s) 314
KroI GCCGGC 1 cut(s) 593
KroNI GCCGGC 1 cut(s) 595
Kzo9I GATC 1 cut(s) 552
LmnI GCTCC 2 cut(s) 605, 785
LpnPI CCDG 9 cut(s) 22, 238, 327, 586, 607, 740, 790, 794, 818
Lsp1109I GCAGC 2 cut(s) 289, 609
LweI GCATC 1 cut(s) 825
MaeI CTAG 4 cut(s) 92, 150, 254, 327
MaeII ACGT 2 cut(s) 561, 695
MaeIII GTNAC 4 cut(s) 136, 691, 715, 843
MalI GATC 1 cut(s) 554
MbiI CCGCTC 1 cut(s) 624
MboI GATC 1 cut(s) 552
MboII GAAGA 3 cut(s) 415, 487, 533
MflI RGATCY 1 cut(s) 552
MluCI AATT 3 cut(s) 168, 392, 501
MlyI GAGTC 2 cut(s) 144, 244
MroNI GCCGGC 1 cut(s) 593
MseI TTAA 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 4 cut(s) 573, 594, 805, 848
Mva1269I GAATGC 1 cut(s) 584
MwoI GCNNNNNNNGC 3 cut(s) 564, 573, 590
NaeI GCCGGC 1 cut(s) 595
NdeII GATC 1 cut(s) 552
NgoMIV GCCGGC 1 cut(s) 593
NlaIII CATG 3 cut(s) 164, 281, 655
NlaIV GGNNCC 2 cut(s) 312, 734
NmeAIII GCCGAG 1 cut(s) 726
NmuCI GTSAC 4 cut(s) 136, 691, 715, 843
NspI RCATGY 1 cut(s) 281
PciI ACATGT 1 cut(s) 277
PcsI WCGNNNNNNNCGW 2 cut(s) 720, 798
PctI GAATGC 1 cut(s) 584
PdiI GCCGGC 1 cut(s) 595
PfeI GAWTC 2 cut(s) 271, 373
PflMI CCANNNNNTGG 2 cut(s) 320, 383
PinAI ACCGGT 1 cut(s) 847
PkrI GCNGC 5 cut(s) 304, 599, 796, 799, 815
PleI GAGTC 2 cut(s) 143, 244
PpsI GAGTC 2 cut(s) 143, 244
PscI ACATGT 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 312, 734
PspPI GGNCC 3 cut(s) 458, 732, 850
PsuI RGATCY 1 cut(s) 552
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 206, 312
RsaNI GTAC 2 cut(s) 205, 311
SalI GTCGAC 1 cut(s) 247
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 5 cut(s) 303, 598, 795, 798, 814
Sau3AI GATC 1 cut(s) 552
Sau96I GGNCC 3 cut(s) 458, 732, 850
SchI GAGTC 2 cut(s) 144, 244
SfaNI GCATC 1 cut(s) 825
SinI GGWCC 2 cut(s) 732, 850
SmlI CTYRAG 3 cut(s) 485, 494, 839
SmoI CTYRAG 3 cut(s) 485, 494, 839
Sse9I AATT 3 cut(s) 168, 392, 501
SspI AATATT 1 cut(s) 353
SspMI CTAG 4 cut(s) 92, 150, 254, 327
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 2 cut(s) 204, 691
TaiI ACGT 2 cut(s) 564, 698
TaqI TCGA 7 cut(s) 56, 84, 123, 235, 248, 427, 615
TasI AATT 3 cut(s) 168, 392, 501
TatI WGTACW 1 cut(s) 204
TauI GCSGC 3 cut(s) 797, 800, 816
TfiI GAWTC 2 cut(s) 271, 373
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 3 cut(s) 207, 321, 772
TseFI GTSAC 4 cut(s) 136, 691, 715, 843
TseI GCWGC 2 cut(s) 302, 597
Tsp45I GTSAC 4 cut(s) 136, 691, 715, 843
TspDTI ATGAA 2 cut(s) 189, 644
TspGWI ACGGA 2 cut(s) 598, 712
TspRI CASTG 3 cut(s) 207, 321, 772
Van91I CCANNNNNTGG 2 cut(s) 320, 383
VpaK11BI GGWCC 2 cut(s) 732, 850
XbaI TCTAGA 1 cut(s) 253
XceI RCATGY 1 cut(s) 281
XmaJI CCTAGG 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 248
XspI CTAG 4 cut(s) 92, 150, 254, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.