Rroxscaffold_2G00118670

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012676
Physical Location & Seq
Forward (+)
50331810 .. 50345953
14144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118670.1

Sequence Viewer

Length: 813 bp
ATGGCCAACCGAAAATTGGGGGCGATTTCCGAGCTCTTTGAGTATTACATATTAAAGCAATCTAAGTATCAGCTCCAAATCTCACATATTCAATGGATCTCCCGGATTATTTCTACTTCGCCGTTGTCTCTCTCCGCCGACTCCGATCTCTCGCAGATTACTATCACCGCCGACGAATTCCATTGCTTCTCTCACAGATTGATAGCGACGACTTCGTCTCCGGCTTCGAAGGCAAGTCCAACATCTTCTAGCGGCTTGATACTAGATTTCGGACTCTCTCCTCTTTCTCTCAATTCCATCGACCACATCTCCGTCTCGGGCTCCTCGACGACTCCCTCCCTCCACGGTTCCGTCCTCCAAGGCAAGCACAATCGAACGCCGGCGAGTCCAAACCATAGCGCCGCGGCAGCTGAGTGTATGAAGCTTACAATATTTGAGATGAAGCAAAAAGGAAATCATGTTATGGTTGGAGCATTCAACTTTGTAGCAGATAAAAACAAAAAGAAGCGGAACCGTGCGACTTCGAATCAAAAGAGTTGTTACCACTATAGCAGCCCACCGGCTCGGCCCGGCGGTAGTATGGACATAATCAATCAAGTACTGCAAGCCCTTAACCGGCAAGTAGAAATCGCCGCCGCAATCGCCACCTCCTTCACCACCGGCGGCGCGAAGTCACGGCGTGCACCGCCGTGTCCGACGAGAATCTCGGCACATCACGGAATACCCCAATCTTATCCGCCCTCCCCCGCCCGCCGTCGACATATTCCGAGCAAACTCCGAATCCGGCCGGCGGCGATGAAAGCGAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

29.24

Weight (kDa)

10.24

Isoelectric Point (pI)

67.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 757
AccII CGCG 2 cut(s) 404, 668
AclWI GGATC 1 cut(s) 104
AcoI YGGCCR 2 cut(s) 3, 785
AcsI RAATTY 1 cut(s) 176
AdeI CACNNNGTG 1 cut(s) 680
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 3 cut(s) 16, 615, 790
AgsI TTSAA 2 cut(s) 92, 478
AleI CACNNNNGTG 1 cut(s) 688
AluBI AGCT 4 cut(s) 34, 73, 410, 424
AluI AGCT 4 cut(s) 34, 73, 410, 424
Alw21I GWGCWC 2 cut(s) 36, 685
Alw26I GTCTC 3 cut(s) 132, 222, 319
Alw44I GTGCAC 1 cut(s) 681
AlwI GGATC 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 316
AoxI GGCC 3 cut(s) 3, 566, 785
ApaLI GTGCAC 1 cut(s) 681
ApeKI GCWGC 2 cut(s) 407, 552
ApoI RAATTY 1 cut(s) 176
AspLEI GCGC 2 cut(s) 401, 668
AspS9I GGNCC 1 cut(s) 567
AsuC2I CCSGG 2 cut(s) 103, 570
AsuHPI GGTGA 2 cut(s) 157, 646
AsuII TTCGAA 2 cut(s) 227, 524
AvaI CYCGRG 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 685
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 36, 323
Bbv12I GWGCWC 2 cut(s) 36, 685
BbvI GCAGC 2 cut(s) 419, 564
BccI CCATC 1 cut(s) 305
BceAI ACGGC 4 cut(s) 106, 673, 692, 738
BcnI CCSGG 2 cut(s) 103, 570
BcoDI GTCTC 3 cut(s) 132, 222, 319
BfaI CTAG 2 cut(s) 249, 263
BfmI CTRYAG 1 cut(s) 547
BfoI RGCGCY 1 cut(s) 402
BisI GCNGC 9 cut(s) 253, 402, 405, 408, 553, 633, 636, 664, 792
BlsI GCNGC 9 cut(s) 254, 403, 406, 409, 554, 634, 637, 665, 793
BmcAI AGTACT 1 cut(s) 600
Bme1390I CCNGG 2 cut(s) 103, 570
BmeT110I CYCGRG 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 567
BmiI GGNNCC 3 cut(s) 322, 349, 512
BmrFI CCNGG 2 cut(s) 103, 570
Bpu14I TTCGAA 2 cut(s) 227, 524
BpuMI CCSGG 2 cut(s) 103, 570
BsaBI GATNNNNATC 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 343, 358, 402
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 293, 323
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 615, 790
Bse118I RCCGGY 5 cut(s) 379, 559, 615, 659, 787
Bse3DI GCAATG 1 cut(s) 181
Bse8I GATNNNNATC 1 cut(s) 161
BseDI CCNNGG 3 cut(s) 343, 358, 402
BseJI GATNNNNATC 1 cut(s) 161
BseLI CCNNNNNNNGG 3 cut(s) 16, 615, 790
BseMI GCAATG 1 cut(s) 181
BseMII CTCAG 1 cut(s) 402
BseRI GAGGAG 2 cut(s) 270, 313
BseSI GKGCMC 1 cut(s) 685
BseX3I CGGCCG 1 cut(s) 785
BseXI GCAGC 2 cut(s) 419, 564
Bsh1236I CGCG 2 cut(s) 404, 668
Bsh1285I CGRYCG 1 cut(s) 788
BshFI GGCC 3 cut(s) 5, 568, 787
BsiEI CGRYCG 1 cut(s) 788
BsiHKAI GWGCWC 2 cut(s) 36, 685
BsiHKCI CYCGRG 1 cut(s) 316
BsiSI CCGG 9 cut(s) 103, 221, 380, 560, 570, 616, 660, 784, 788
BslI CCNNNNNNNGG 3 cut(s) 16, 615, 790
BsmAI GTCTC 3 cut(s) 132, 222, 319
BsmBI CGTCTC 2 cut(s) 222, 319
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 3 cut(s) 5, 568, 787
BsoBI CYCGRG 1 cut(s) 316
Bsp119I TTCGAA 2 cut(s) 227, 524
Bsp1286I GDGCHC 3 cut(s) 36, 323, 685
Bsp143I GATC 2 cut(s) 96, 145
BspANI GGCC 3 cut(s) 5, 568, 787
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 2 cut(s) 404, 668
BspLI GGNNCC 3 cut(s) 322, 349, 512
BspPI GGATC 1 cut(s) 104
BspT104I TTCGAA 2 cut(s) 227, 524
BsrDI GCAATG 1 cut(s) 181
BsrFI RCCGGY 5 cut(s) 379, 559, 615, 659, 787
BssAI RCCGGY 5 cut(s) 379, 559, 615, 659, 787
BssECI CCNNGG 3 cut(s) 343, 358, 402
BssMI GATC 2 cut(s) 96, 145
BssT1I CCWWGG 1 cut(s) 358
Bst4CI ACNGT 2 cut(s) 347, 515
BstBI TTCGAA 2 cut(s) 227, 524
BstC8I GCNNGC 7 cut(s) 365, 381, 606, 681, 751, 789, 805
BstDEI CTNAG 2 cut(s) 63, 411
BstDSI CCRYGG 2 cut(s) 343, 402
BstFNI CGCG 2 cut(s) 404, 668
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 2 cut(s) 401, 668
BstKTI GATC 2 cut(s) 99, 148
BstMAI GTCTC 3 cut(s) 132, 222, 319
BstMBI GATC 2 cut(s) 96, 145
BstMCI CGRYCG 1 cut(s) 788
BstMWI GCNNNNNNNGC 5 cut(s) 230, 407, 641, 685, 800
BstSCI CCNGG 2 cut(s) 101, 568
BstSFI CTRYAG 1 cut(s) 547
BstSLI GKGCMC 1 cut(s) 685
BstUI CGCG 2 cut(s) 404, 668
BstV1I GCAGC 2 cut(s) 419, 564
BstX2I RGATCY 1 cut(s) 96
BstYI RGATCY 1 cut(s) 96
BstZI CGGCCG 1 cut(s) 785
BsuRI GGCC 3 cut(s) 5, 568, 787
BtgI CCRYGG 2 cut(s) 343, 402
BtgZI GCGATG 1 cut(s) 809
Cac8I GCNNGC 7 cut(s) 365, 381, 606, 681, 751, 789, 805
CfoI GCGC 2 cut(s) 401, 668
Cfr10I RCCGGY 5 cut(s) 379, 559, 615, 659, 787
Cfr13I GGNCC 1 cut(s) 567
Cfr42I CCGCGG 1 cut(s) 405
Csp6I GTAC 1 cut(s) 599
CviAII CATG 1 cut(s) 458
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 2 cut(s) 63, 411
DpnI GATC 2 cut(s) 98, 147
DpnII GATC 2 cut(s) 96, 145
DraIII CACNNNGTG 1 cut(s) 680
DrdI GACNNNNNNGTC 1 cut(s) 214
DseDI GACNNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 3, 785
EagI CGGCCG 1 cut(s) 785
EciI GGCGGA 2 cut(s) 124, 726
Ecl136II GAGCTC 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 785
Eco130I CCWWGG 1 cut(s) 358
Eco24I GRGCYC 2 cut(s) 36, 323
Eco52I CGGCCG 1 cut(s) 785
Eco53kI GAGCTC 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 316
EcoICRI GAGCTC 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 358
EcoT38I GRGCYC 2 cut(s) 36, 323
ErhI CCWWGG 1 cut(s) 358
Esp3I CGTCTC 2 cut(s) 222, 319
FaeI CATG 1 cut(s) 461
FatI CATG 1 cut(s) 457
FauI CCCGC 2 cut(s) 754, 758
FblI GTMKAC 1 cut(s) 757
Fnu4HI GCNGC 9 cut(s) 253, 402, 405, 408, 553, 633, 636, 664, 792
FriOI GRGCYC 2 cut(s) 36, 323
Fsp4HI GCNGC 9 cut(s) 253, 402, 405, 408, 553, 633, 636, 664, 792
FspBI CTAG 2 cut(s) 249, 263
GlaI GCGC 2 cut(s) 400, 667
GluI GCNGC 9 cut(s) 253, 402, 405, 408, 553, 633, 636, 664, 792
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 3 cut(s) 5, 568, 787
HapII CCGG 9 cut(s) 103, 221, 380, 560, 570, 616, 660, 784, 788
HhaI GCGC 2 cut(s) 401, 668
Hin1II CATG 1 cut(s) 461
Hin6I GCGC 2 cut(s) 399, 666
HinP1I GCGC 2 cut(s) 399, 666
HincII GTYRAC 1 cut(s) 758
HindII GTYRAC 1 cut(s) 758
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 7 cut(s) 140, 273, 331, 385, 526, 702, 780
HpaII CCGG 9 cut(s) 103, 221, 380, 560, 570, 616, 660, 784, 788
HphI GGTGA 2 cut(s) 157, 646
Hpy166II GTNNAC 2 cut(s) 683, 758
Hpy188I TCNGA 6 cut(s) 31, 145, 272, 696, 768, 779
Hpy8I GTNNAC 2 cut(s) 683, 758
Hpy99I CGWCG 5 cut(s) 176, 211, 331, 700, 759
HpyAV CCTTC 2 cut(s) 223, 661
HpyCH4III ACNGT 2 cut(s) 347, 515
HpyCH4V TGCA 2 cut(s) 604, 683
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 407, 641, 685, 800
HpyF3I CTNAG 2 cut(s) 63, 411
Hsp92II CATG 1 cut(s) 461
HspAI GCGC 2 cut(s) 399, 666
KroI GCCGGC 2 cut(s) 379, 787
KroNI GCCGGC 2 cut(s) 381, 789
KspI CCGCGG 1 cut(s) 405
Kzo9I GATC 2 cut(s) 96, 145
LmnI GCTCC 3 cut(s) 78, 326, 470
LpnPI CCDG 9 cut(s) 116, 234, 393, 573, 583, 629, 673, 797, 801
Lsp1109I GCAGC 2 cut(s) 419, 564
MaeI CTAG 2 cut(s) 249, 263
MaeIII GTNAC 2 cut(s) 539, 672
MalI GATC 2 cut(s) 98, 147
MboI GATC 2 cut(s) 96, 145
MboII GAAGA 1 cut(s) 237
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 3 cut(s) 36, 323, 685
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 14, 176, 292
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 134, 267, 325, 394
MmeI TCCRAC 3 cut(s) 263, 448, 719
MnlI CCTC 7 cut(s) 291, 334, 346, 350, 365, 658, 751
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 379
MroNI GCCGGC 2 cut(s) 379, 787
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 53, 612
MslI CAYNNNNRTG 1 cut(s) 688
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 404, 410
MspI CCGG 9 cut(s) 103, 221, 380, 560, 570, 616, 660, 784, 788
MspR9I CCNGG 2 cut(s) 103, 570
Mva1269I GAATGC 1 cut(s) 473
MvnI CGCG 2 cut(s) 404, 668
MwoI GCNNNNNNNGC 5 cut(s) 230, 407, 641, 685, 800
NaeI GCCGGC 2 cut(s) 381, 789
NciI CCSGG 2 cut(s) 103, 570
NdeII GATC 2 cut(s) 96, 145
NgoMIV GCCGGC 2 cut(s) 379, 787
NlaIII CATG 1 cut(s) 461
NlaIV GGNNCC 3 cut(s) 322, 349, 512
NmeAIII GCCGAG 2 cut(s) 544, 686
NmuCI GTSAC 1 cut(s) 672
NspV TTCGAA 2 cut(s) 227, 524
OliI CACNNNNGTG 1 cut(s) 688
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PctI GAATGC 1 cut(s) 473
PdiI GCCGGC 2 cut(s) 381, 789
PfeI GAWTC 3 cut(s) 526, 702, 780
PflFI GACNNNGTC 1 cut(s) 214
PfoI TCCNGGA 1 cut(s) 101
PkrI GCNGC 9 cut(s) 254, 403, 406, 409, 554, 634, 637, 665, 793
PleI GAGTC 4 cut(s) 134, 267, 325, 393
PpsI GAGTC 4 cut(s) 134, 267, 325, 393
Psp124BI GAGCTC 1 cut(s) 36
PspN4I GGNNCC 3 cut(s) 322, 349, 512
PspPI GGNCC 1 cut(s) 567
PsuI RGATCY 1 cut(s) 96
PsyI GACNNNGTC 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
RseI CAYNNNNRTG 1 cut(s) 688
SacI GAGCTC 1 cut(s) 36
SacII CCGCGG 1 cut(s) 405
SalI GTCGAC 1 cut(s) 756
SaqAI TTAA 2 cut(s) 53, 612
SatI GCNGC 9 cut(s) 253, 402, 405, 408, 553, 633, 636, 664, 792
Sau3AI GATC 2 cut(s) 96, 145
Sau96I GGNCC 1 cut(s) 567
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 4 cut(s) 134, 267, 325, 394
ScrFI CCNGG 2 cut(s) 103, 570
SduI GDGCHC 3 cut(s) 36, 323, 685
SetI ASST 5 cut(s) 36, 75, 412, 426, 650
SfcI CTRYAG 1 cut(s) 547
Sfr303I CCGCGG 1 cut(s) 405
SfuI TTCGAA 2 cut(s) 227, 524
SgrAI CRCCGGYG 2 cut(s) 379, 659
SgrBI CCGCGG 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 688
Sse9I AATT 3 cut(s) 14, 176, 292
SspI AATATT 1 cut(s) 432
SspMI CTAG 2 cut(s) 249, 263
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 2 cut(s) 101, 568
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 2 cut(s) 347, 515
TaqI TCGA 6 cut(s) 227, 300, 326, 373, 524, 757
TasI AATT 3 cut(s) 14, 176, 292
TatI WGTACW 1 cut(s) 598
TauI GCSGC 7 cut(s) 255, 404, 407, 635, 638, 666, 794
TfiI GAWTC 3 cut(s) 526, 702, 780
Tru1I TTAA 2 cut(s) 53, 612
Tru9I TTAA 2 cut(s) 53, 612
TseFI GTSAC 1 cut(s) 672
TseI GCWGC 2 cut(s) 407, 552
Tsp45I GTSAC 1 cut(s) 672
TspDTI ATGAA 3 cut(s) 434, 455, 812
TspGWI ACGGA 3 cut(s) 301, 340, 732
Tth111I GACNNNGTC 1 cut(s) 214
VneI GTGCAC 1 cut(s) 681
XapI RAATTY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 757
XspI CTAG 2 cut(s) 249, 263
ZrmI AGTACT 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.