Rroxscaffold_1G00016470

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012676
Physical Location & Seq
Reverse (-)
20472080 .. 20487098
15019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00016470.1

Sequence Viewer

Length: 690 bp
ATGACCGCCTCCAACTCCAATTCTCTCCCTCCATCTCGGCCGTGGCCGCCGTGCACGAATCACCCACCGGTCCAAGTCGTTCCTCAAGTACCTAATACGGAGCCCTACCCCAAATCCAAGACTCTAAATCAACAACTCCAAATCTCACATAATCAACTGGATCTCCCCGATTCTTTCGATTTCACCGAGGACCGCGACAATGATAAAGGTGCACGGCGAGGCAAGACAGCCAGGGCATCGACGACGTCGTCTAGGGCTCGGTTGGTGTCGACTCCTTCAAGCCTCCCGATTTTCAAGCCACCCGATTCTCATGAAGGTCCCTTCGAGCGTCAAATTAGGAAAACCAAGGTTTCGGTCACAAGTGATTTGGAAATCGAGTATCTCCGAGCTCAAAACCTTCACCGGCACCATCCACCGTCCAAGACCTGCCGGGACCGCATGCTCACCGGCCCACCCGGCCGCAACAACTTCGGATCCGCCGACGTCGCCCTCGGCCTCCTCGTATTCCCCGCCGGCGGCTCCATCTCCGTCATCAACACCCGTTCAATGCGGCTCGTCGTCTCCCTCCCCACCCCACTCCCTCTCGCCGCAACCTCCTCTCCACTAAGCCTTCCGGCTCCCACCTCCTCCTTGCGCCGTGGCCGCATCGCCCTCCTCGACCTCCGGCTCATGTCGCCGGTCCTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

25.09

Weight (kDa)

10.36

Isoelectric Point (pI)

57.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR11_1st PF23751 150 - 201 3.1e-12 WDR11 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 247
AatII GACGTC 2 cut(s) 248, 486
Acc36I ACCTGC 1 cut(s) 434
AccB1I GGYRCC 1 cut(s) 405
AccI GTMKAC 1 cut(s) 269
AccII CGCG 1 cut(s) 195
AclWI GGATC 3 cut(s) 168, 468, 481
AcoI YGGCCR 4 cut(s) 38, 44, 457, 640
AcyI GRCGYC 2 cut(s) 245, 483
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 515
AgeI ACCGGT 1 cut(s) 67
AgsI TTSAA 3 cut(s) 279, 295, 546
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 1 cut(s) 389
AluI AGCT 1 cut(s) 389
Alw21I GWGCWC 3 cut(s) 56, 214, 391
Alw26I GTCTC 1 cut(s) 565
Alw44I GTGCAC 2 cut(s) 52, 210
AlwI GGATC 3 cut(s) 168, 468, 481
AoxI GGCC 6 cut(s) 38, 44, 448, 457, 493, 640
ApaLI GTGCAC 2 cut(s) 52, 210
AsiGI ACCGGT 1 cut(s) 67
AspLEI GCGC 1 cut(s) 636
AspS9I GGNCC 6 cut(s) 70, 190, 317, 433, 449, 679
AsuC2I CCSGG 2 cut(s) 431, 456
AsuHPI GGTGA 4 cut(s) 53, 175, 392, 436
AvaII GGWCC 5 cut(s) 70, 190, 317, 433, 679
BaeGI GKGCMC 2 cut(s) 56, 214
BamHI GGATCC 1 cut(s) 473
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 3 cut(s) 105, 259, 391
Bbv12I GWGCWC 3 cut(s) 56, 214, 391
BccI CCATC 3 cut(s) 40, 417, 530
BceAI ACGGC 4 cut(s) 25, 34, 230, 621
BcgI CGANNNNNNTGC 2 cut(s) 451, 485
BciT130I CCWGG 1 cut(s) 232
BcnI CCSGG 2 cut(s) 431, 456
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 434
BglI GCCNNNNNGGC 1 cut(s) 456
BisI GCNGC 6 cut(s) 47, 460, 517, 551, 588, 643
BlsI GCNGC 6 cut(s) 48, 461, 518, 552, 589, 644
Bme1390I CCNGG 3 cut(s) 232, 431, 456
Bme18I GGWCC 5 cut(s) 70, 190, 317, 433, 679
BmgT120I GGNCC 6 cut(s) 70, 190, 317, 433, 449, 679
BmiI GGNNCC 7 cut(s) 102, 319, 407, 434, 475, 520, 618
BmrFI CCNGG 3 cut(s) 232, 431, 456
BmsI GCATC 2 cut(s) 245, 654
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 2 cut(s) 431, 456
BsaHI GRCGYC 2 cut(s) 245, 483
BsaJI CCNNGG 6 cut(s) 41, 186, 231, 345, 490, 637
BsaWI WCCGGW 1 cut(s) 67
BsaXI ACNNNNNCTCC 4 cut(s) 147, 177, 583, 613
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse118I RCCGGY 5 cut(s) 67, 402, 446, 512, 676
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 6 cut(s) 41, 186, 231, 345, 490, 637
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 1 cut(s) 515
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 4 cut(s) 488, 586, 616, 644
BseSI GKGCMC 2 cut(s) 56, 214
BseX3I CGGCCG 2 cut(s) 38, 457
Bsh1236I CGCG 1 cut(s) 195
Bsh1285I CGRYCG 2 cut(s) 41, 460
BshFI GGCC 6 cut(s) 40, 46, 450, 459, 495, 642
BshNI GGYRCC 1 cut(s) 405
BshTI ACCGGT 1 cut(s) 67
BsiEI CGRYCG 2 cut(s) 41, 460
BsiHKAI GWGCWC 3 cut(s) 56, 214, 391
BsiSI CCGG 9 cut(s) 68, 403, 430, 447, 456, 513, 614, 664, 677
BslFI GGGAC 2 cut(s) 303, 446
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 1 cut(s) 565
BsmBI CGTCTC 1 cut(s) 565
BsmFI GGGAC 2 cut(s) 303, 446
BsnI GGCC 6 cut(s) 40, 46, 450, 459, 495, 642
Bsp1286I GDGCHC 5 cut(s) 56, 105, 214, 259, 391
Bsp143I GATC 2 cut(s) 160, 473
BspANI GGCC 6 cut(s) 40, 46, 450, 459, 495, 642
BspFNI CGCG 1 cut(s) 195
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 7 cut(s) 102, 319, 407, 434, 475, 520, 618
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 3 cut(s) 168, 468, 481
BspT107I GGYRCC 1 cut(s) 405
BsrFI RCCGGY 5 cut(s) 67, 402, 446, 512, 676
BsrI ACTGG 1 cut(s) 162
BssAI RCCGGY 5 cut(s) 67, 402, 446, 512, 676
BssECI CCNNGG 6 cut(s) 41, 186, 231, 345, 490, 637
BssMI GATC 2 cut(s) 160, 473
BssNI GRCGYC 2 cut(s) 245, 483
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 1 cut(s) 417
BstACI GRCGYC 2 cut(s) 245, 483
BstC8I GCNNGC 2 cut(s) 440, 514
BstDEI CTNAG 1 cut(s) 605
BstDSI CCRYGG 2 cut(s) 41, 637
BstF5I GGATG 1 cut(s) 409
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 636
BstKTI GATC 2 cut(s) 163, 476
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 2 cut(s) 160, 473
BstMCI CGRYCG 2 cut(s) 41, 460
BstMWI GCNNNNNNNGC 6 cut(s) 46, 435, 456, 485, 642, 673
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 3 cut(s) 230, 429, 454
BstSLI GKGCMC 2 cut(s) 56, 214
BstUI CGCG 1 cut(s) 195
BstX2I RGATCY 2 cut(s) 160, 473
BstYI RGATCY 2 cut(s) 160, 473
BstZI CGGCCG 2 cut(s) 38, 457
BsuRI GGCC 6 cut(s) 40, 46, 450, 459, 495, 642
BtgI CCRYGG 2 cut(s) 41, 637
BtgZI GCGATG 1 cut(s) 631
BtsCI GGATG 1 cut(s) 409
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 2 cut(s) 440, 514
CciI TCATGA 1 cut(s) 310
CfoI GCGC 1 cut(s) 636
Cfr10I RCCGGY 5 cut(s) 67, 402, 446, 512, 676
Cfr13I GGNCC 6 cut(s) 70, 190, 317, 433, 449, 679
CseI GACGC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 89
CspAI ACCGGT 1 cut(s) 67
CviAII CATG 3 cut(s) 311, 439, 670
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 1 cut(s) 605
DpnI GATC 2 cut(s) 162, 475
DpnII GATC 2 cut(s) 160, 473
DrdI GACNNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 247
EaeI YGGCCR 4 cut(s) 38, 44, 457, 640
EagI CGGCCG 2 cut(s) 38, 457
EciI GGCGGA 1 cut(s) 466
Ecl136II GAGCTC 1 cut(s) 389
EclXI CGGCCG 2 cut(s) 38, 457
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 3 cut(s) 105, 259, 391
Eco47I GGWCC 5 cut(s) 70, 190, 317, 433, 679
Eco52I CGGCCG 2 cut(s) 38, 457
Eco53kI GAGCTC 1 cut(s) 389
EcoICRI GAGCTC 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 3 cut(s) 105, 259, 391
ErhI CCWWGG 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 565
FaeI CATG 3 cut(s) 314, 442, 673
FaiI YATR 5 cut(s) 150, 312, 440, 671, 685
FaqI GGGAC 2 cut(s) 303, 446
FatI CATG 3 cut(s) 310, 438, 669
FauI CCCGC 1 cut(s) 517
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 6 cut(s) 47, 460, 517, 551, 588, 643
FokI GGATG 1 cut(s) 396
FriOI GRGCYC 3 cut(s) 105, 259, 391
Fsp4HI GCNGC 6 cut(s) 47, 460, 517, 551, 588, 643
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 635
GluI GCNGC 6 cut(s) 47, 460, 517, 551, 588, 643
HaeIII GGCC 6 cut(s) 40, 46, 450, 459, 495, 642
HapII CCGG 9 cut(s) 68, 403, 430, 447, 456, 513, 614, 664, 677
HgaI GACGC 1 cut(s) 317
HhaI GCGC 1 cut(s) 636
Hin1I GRCGYC 2 cut(s) 245, 483
Hin1II CATG 3 cut(s) 314, 442, 673
Hin6I GCGC 1 cut(s) 634
HinP1I GCGC 1 cut(s) 634
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HinfI GANTC 5 cut(s) 58, 121, 170, 271, 305
HpaII CCGG 9 cut(s) 68, 403, 430, 447, 456, 513, 614, 664, 677
HphI GGTGA 4 cut(s) 53, 175, 392, 436
Hpy166II GTNNAC 3 cut(s) 54, 212, 270
Hpy188I TCNGA 2 cut(s) 386, 473
Hpy188III TCNNGA 2 cut(s) 286, 311
Hpy8I GTNNAC 3 cut(s) 54, 212, 270
Hpy99I CGWCG 6 cut(s) 244, 247, 250, 485, 488, 560
HpyAV CCTTC 5 cut(s) 285, 308, 331, 407, 620
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 2 cut(s) 245, 483
HpyCH4V TGCA 2 cut(s) 54, 212
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 435, 456, 485, 642, 673
HpyF3I CTNAG 1 cut(s) 605
HpySE526I ACGT 2 cut(s) 245, 483
Hsp92I GRCGYC 2 cut(s) 245, 483
Hsp92II CATG 3 cut(s) 314, 442, 673
HspAI GCGC 1 cut(s) 634
KroI GCCGGC 1 cut(s) 512
KroNI GCCGGC 1 cut(s) 514
Kzo9I GATC 2 cut(s) 160, 473
LmnI GCTCC 3 cut(s) 100, 524, 622
LweI GCATC 2 cut(s) 245, 654
MaeI CTAG 1 cut(s) 252
MaeII ACGT 2 cut(s) 245, 483
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 162, 475
MboI GATC 2 cut(s) 160, 473
MflI RGATCY 2 cut(s) 160, 473
MhlI GDGCHC 5 cut(s) 56, 105, 214, 259, 391
MluCI AATT 2 cut(s) 19, 333
MlyI GAGTC 2 cut(s) 115, 265
MmeI TCCRAC 1 cut(s) 36
MreI CGCCGGCG 1 cut(s) 512
MroNI GCCGGC 1 cut(s) 512
MspI CCGG 9 cut(s) 68, 403, 430, 447, 456, 513, 614, 664, 677
MspR9I CCNGG 3 cut(s) 232, 431, 456
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 6 cut(s) 46, 435, 456, 485, 642, 673
NaeI GCCGGC 1 cut(s) 514
NciI CCSGG 2 cut(s) 431, 456
NdeII GATC 2 cut(s) 160, 473
NgoMIV GCCGGC 1 cut(s) 512
NlaIII CATG 3 cut(s) 314, 442, 673
NlaIV GGNNCC 7 cut(s) 102, 319, 407, 434, 475, 520, 618
NmeAIII GCCGAG 2 cut(s) 16, 471
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 1 cut(s) 442
PaeI GCATGC 1 cut(s) 442
PagI TCATGA 1 cut(s) 310
PcsI WCGNNNNNNNCGW 5 cut(s) 183, 245, 477, 498, 654
PdiI GCCGGC 1 cut(s) 514
PfeI GAWTC 3 cut(s) 58, 170, 305
PflFI GACNNNGTC 2 cut(s) 244, 247
PinAI ACCGGT 1 cut(s) 67
PkrI GCNGC 6 cut(s) 48, 461, 518, 552, 589, 644
PleI GAGTC 2 cut(s) 115, 265
PpsI GAGTC 2 cut(s) 115, 265
PpuMI RGGWCCY 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 391
Psp5II RGGWCCY 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 7 cut(s) 102, 319, 407, 434, 475, 520, 618
PspPI GGNCC 6 cut(s) 70, 190, 317, 433, 449, 679
PspPPI RGGWCCY 1 cut(s) 317
PsuI RGATCY 2 cut(s) 160, 473
PsyI GACNNNGTC 2 cut(s) 244, 247
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
SacI GAGCTC 1 cut(s) 391
SalI GTCGAC 1 cut(s) 268
SatI GCNGC 6 cut(s) 47, 460, 517, 551, 588, 643
Sau3AI GATC 2 cut(s) 160, 473
Sau96I GGNCC 6 cut(s) 70, 190, 317, 433, 449, 679
SchI GAGTC 2 cut(s) 115, 265
ScrFI CCNGG 3 cut(s) 232, 431, 456
SduI GDGCHC 5 cut(s) 56, 105, 214, 259, 391
SfaNI GCATC 2 cut(s) 245, 654
SfiI GGCCNNNNNGGCC 1 cut(s) 456
SgrAI CRCCGGYG 1 cut(s) 512
SinI GGWCC 5 cut(s) 70, 190, 317, 433, 679
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SphI GCATGC 1 cut(s) 442
Sse9I AATT 2 cut(s) 19, 333
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 391
StyD4I CCNGG 3 cut(s) 230, 429, 454
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 2 cut(s) 248, 486
TaqI TCGA 6 cut(s) 177, 239, 269, 324, 375, 657
TaqII GACCGA 1 cut(s) 343
TasI AATT 2 cut(s) 19, 333
TauI GCSGC 6 cut(s) 49, 462, 519, 553, 590, 645
TfiI GAWTC 3 cut(s) 58, 170, 305
TseFI GTSAC 1 cut(s) 355
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 1 cut(s) 327
TspGWI ACGGA 2 cut(s) 113, 517
Tth111I GACNNNGTC 2 cut(s) 244, 247
VneI GTGCAC 2 cut(s) 52, 210
VpaK11BI GGWCC 5 cut(s) 70, 190, 317, 433, 679
XceI RCATGY 1 cut(s) 442
XcmI CCANNNNNNNNNTGG 1 cut(s) 39
XmiI GTMKAC 1 cut(s) 269
XspI CTAG 1 cut(s) 252
ZraI GACGTC 2 cut(s) 246, 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.