Rmu_sc0000654.1_g000028

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000654.1
OG0009691
Physical Location & Seq
Forward (+)
125796 .. 129840
4045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000654.1_g000028.1.cds

Sequence Viewer

Length: 1059 bp
atggtggaggttcttgatcttgaatcagaccttgatggggaggaggtggttgtgcctgagcttaatcagaacgatgctaaaagctatgaacatgtccaaaagatgattcaagacgaacagattggtaaactgaaagtggagcaatgtaagctctacttgaggaaaaatgggttgagactgactggcaataaggacacgctcattcagcggattaaggagcatctagagattttgaatggtggaggtgagaagaagtaccctgcatctagctttgttttgaattgcaaagttcggactgctactgacgtgaaaatagaaaaccactgcacagaccagacgctcaacaggctgaagaagataaaaagggaaggcgttgaaacttgtacctgctcaagggttagaaacatgatcacattccaagatcctagccgtccaaaatttagagagaagtcaccttccaccttgctgtgctccggtgaccagtggccggcgcggtctccggcaaccggactccggcgaacggtgaccggattccggcaaccaatgaccggattccgggaaccggtaaccgaaatccagcgaccgatgactggaatccggaatccggctattattgccccccaatcgaaaatttccggtcgtccactgattcaactcttggtttgctcctccaaaccagcacacctgagaatcggcggggcagaaggcacagcagagttcgatgagggcgacagggtaggctctacaggtggaatcgaattgatccatgacggagtcgagatctggggaggaagggcagtggcatattccgatgatggcgagggttttgcggttggattggaagtcctcccaggggctgggaggggcgctgccgaggacgaactagaagttgaggctgccggagtcccagggctggtgagcgaagggtggcttctggtgctcggtgtagatggagctgacggcggagagtggatcgaccggggggacgtggccgatggggaggacctccatgcggatcatggagctggtttcgatgctcacgtccgactccatttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAP zf-CCCH

Protein Analysis

352

Amino Acids

38.38

Weight (kDa)

5.34

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 857
AccII CGCG 1 cut(s) 493
AccIII TCCGGA 1 cut(s) 597
AciI CCGC 6 cut(s) 208, 493, 696, 830, 963, 1013
AclWI GGATC 4 cut(s) 416, 757, 980, 1023
AcoI YGGCCR 2 cut(s) 485, 990
AcsI RAATTY 2 cut(s) 437, 630
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 2 cut(s) 257, 385
AflIII ACRYGT 1 cut(s) 91
AgeI ACCGGT 1 cut(s) 562
AgsI TTSAA 6 cut(s) 23, 110, 235, 280, 377, 653
AjiI CACGTC 3 cut(s) 307, 988, 1042
AjnI CCWGG 2 cut(s) 850, 907
AjuI GAANNNNNNNTTGG 2 cut(s) 90, 122
AluBI AGCT 6 cut(s) 61, 84, 151, 270, 956, 1025
AluI AGCT 6 cut(s) 61, 84, 151, 270, 956, 1025
Alw21I GWGCWC 2 cut(s) 473, 942
Alw26I GTCTC 2 cut(s) 169, 501
AlwI GGATC 4 cut(s) 416, 757, 980, 1023
AlwNI CAGNNNCTG 1 cut(s) 857
Aor13HI TCCGGA 1 cut(s) 597
AoxI GGCC 2 cut(s) 485, 990
ApeKI GCWGC 2 cut(s) 869, 896
ApoI RAATTY 2 cut(s) 437, 630
AsiGI ACCGGT 1 cut(s) 562
AspLEI GCGC 2 cut(s) 493, 869
AspS9I GGNCC 1 cut(s) 1003
AsuC2I CCSGG 2 cut(s) 557, 980
AsuHPI GGTGA 5 cut(s) 257, 444, 488, 535, 928
AvaII GGWCC 1 cut(s) 1003
Bbv12I GWGCWC 2 cut(s) 473, 942
BbvI GCAGC 2 cut(s) 856, 883
BccI CCATC 4 cut(s) 29, 809, 944, 989
BceAI ACGGC 2 cut(s) 414, 976
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 2 cut(s) 852, 909
BclI TGATCA 1 cut(s) 408
BcnI CCSGG 2 cut(s) 557, 980
BcoDI GTCTC 2 cut(s) 169, 501
BfaI CTAG 4 cut(s) 224, 267, 426, 884
BfmI CTRYAG 1 cut(s) 744
BfoI RGCGCY 1 cut(s) 870
BfuAI ACCTGC 1 cut(s) 395
BglII AGATCT 1 cut(s) 780
BisI GCNGC 2 cut(s) 870, 897
BlsI GCNGC 2 cut(s) 871, 898
Bme1390I CCNGG 4 cut(s) 557, 852, 909, 980
Bme18I GGWCC 1 cut(s) 1003
BmgBI CACGTC 3 cut(s) 307, 988, 1042
BmgT120I GGNCC 1 cut(s) 1003
BmiI GGNNCC 1 cut(s) 561
BmrFI CCNGG 4 cut(s) 557, 852, 909, 980
BmsI GCATC 4 cut(s) 64, 229, 272, 1024
Bpu10I CCTNAGC 1 cut(s) 57
BpuEI CTTGAG 2 cut(s) 178, 376
BpuMI CCSGG 2 cut(s) 557, 980
BsaI GGTCTC 1 cut(s) 501
BsaJI CCNNGG 6 cut(s) 850, 851, 873, 907, 908, 979
BsaWI WCCGGW 7 cut(s) 473, 506, 527, 548, 562, 597, 635
Bse118I RCCGGY 2 cut(s) 487, 562
Bse1I ACTGG 3 cut(s) 187, 481, 595
Bse3DI GCAATG 1 cut(s) 149
BseAI TCCGGA 1 cut(s) 597
BseBI CCWGG 2 cut(s) 852, 909
BseDI CCNNGG 6 cut(s) 850, 851, 873, 907, 908, 979
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 2 cut(s) 48, 677
BseNI ACTGG 3 cut(s) 187, 481, 595
BseRI GAGGAG 2 cut(s) 56, 658
BseXI GCAGC 2 cut(s) 856, 883
BseYI CCCAGC 1 cut(s) 857
BsgI GTGCAG 1 cut(s) 310
Bsh1236I CGCG 1 cut(s) 493
Bsh1285I CGRYCG 3 cut(s) 584, 640, 979
BshFI GGCC 2 cut(s) 487, 992
BshTI ACCGGT 1 cut(s) 562
BsiEI CGRYCG 3 cut(s) 584, 640, 979
BsiHKAI GWGCWC 2 cut(s) 473, 942
BslFI GGGAC 2 cut(s) 890, 998
BsmAI GTCTC 2 cut(s) 169, 501
BsmFI GGGAC 2 cut(s) 890, 998
BsnI GGCC 2 cut(s) 487, 992
Bso31I GGTCTC 1 cut(s) 501
Bsp1286I GDGCHC 2 cut(s) 473, 942
Bsp13I TCCGGA 1 cut(s) 597
Bsp143I GATC 7 cut(s) 16, 408, 421, 762, 780, 972, 1015
BspACI CCGC 6 cut(s) 208, 493, 696, 830, 963, 1013
BspANI GGCC 2 cut(s) 487, 992
BspCNI CTCAG 2 cut(s) 49, 678
BspEI TCCGGA 1 cut(s) 597
BspFNI CGCG 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 561
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 4 cut(s) 416, 757, 980, 1023
BspTNI GGTCTC 1 cut(s) 501
BsrDI GCAATG 1 cut(s) 149
BsrFI RCCGGY 2 cut(s) 487, 562
BsrI ACTGG 3 cut(s) 187, 481, 595
BssAI RCCGGY 2 cut(s) 487, 562
BssECI CCNNGG 6 cut(s) 850, 851, 873, 907, 908, 979
BssMI GATC 7 cut(s) 16, 408, 421, 762, 780, 972, 1015
Bst2UI CCWGG 2 cut(s) 852, 909
Bst4CI ACNGT 1 cut(s) 523
BstC8I GCNNGC 1 cut(s) 489
BstDEI CTNAG 2 cut(s) 57, 686
BstEII GGTNACC 3 cut(s) 476, 523, 565
BstENI CCTNNNNNAGG 1 cut(s) 391
BstFNI CGCG 1 cut(s) 493
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 2 cut(s) 493, 869
BstKTI GATC 7 cut(s) 19, 411, 424, 765, 783, 975, 1018
BstMAI GTCTC 2 cut(s) 169, 501
BstMBI GATC 7 cut(s) 16, 408, 421, 762, 780, 972, 1015
BstMCI CGRYCG 3 cut(s) 584, 640, 979
BstMWI GCNNNNNNNGC 5 cut(s) 148, 205, 346, 614, 937
BstNI CCWGG 2 cut(s) 852, 909
BstNSI RCATGY 1 cut(s) 95
BstPI GGTNACC 3 cut(s) 476, 523, 565
BstSCI CCNGG 4 cut(s) 555, 850, 907, 978
BstSFI CTRYAG 1 cut(s) 744
BstUI CGCG 1 cut(s) 493
BstV1I GCAGC 2 cut(s) 856, 883
BstX2I RGATCY 2 cut(s) 421, 780
BstYI RGATCY 2 cut(s) 421, 780
BsuRI GGCC 2 cut(s) 487, 992
BtrI CACGTC 3 cut(s) 307, 988, 1042
BtsI GCAGTG 2 cut(s) 322, 804
BtsIMutI CAGTG 4 cut(s) 322, 488, 644, 804
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 1 cut(s) 489
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 2 cut(s) 493, 869
Cfr10I RCCGGY 2 cut(s) 487, 562
Cfr13I GGNCC 1 cut(s) 1003
CseI GACGC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 256, 384
CspAI ACCGGT 1 cut(s) 562
CviAII CATG 5 cut(s) 92, 406, 767, 1010, 1019
CviQI GTAC 2 cut(s) 256, 384
DdeI CTNAG 2 cut(s) 57, 686
DpnI GATC 7 cut(s) 18, 410, 423, 764, 782, 974, 1017
DpnII GATC 7 cut(s) 16, 408, 421, 762, 780, 972, 1015
EaeI YGGCCR 2 cut(s) 485, 990
EciI GGCGGA 1 cut(s) 978
Eco31I GGTCTC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 1003
Eco57I CTGAAG 1 cut(s) 371
Eco91I GGTNACC 3 cut(s) 476, 523, 565
EcoNI CCTNNNNNAGG 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 1003
EcoO65I GGTNACC 3 cut(s) 476, 523, 565
EcoRII CCWGG 2 cut(s) 850, 907
FaeI CATG 5 cut(s) 95, 409, 770, 1013, 1022
FaiI YATR 7 cut(s) 87, 93, 407, 768, 805, 1011, 1020
FalI AAGNNNNNCTT 4 cut(s) 140, 172, 915, 947
FaqI GGGAC 2 cut(s) 890, 998
FatI CATG 5 cut(s) 91, 405, 766, 1009, 1018
FauI CCCGC 1 cut(s) 689
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 870, 897
Fsp4HI GCNGC 2 cut(s) 870, 897
FspBI CTAG 4 cut(s) 224, 267, 426, 884
GlaI GCGC 2 cut(s) 492, 868
GluI GCNGC 2 cut(s) 870, 897
GsaI CCCAGC 1 cut(s) 861
HaeII RGCGCY 1 cut(s) 870
HaeIII GGCC 2 cut(s) 487, 992
HgaI GACGC 1 cut(s) 346
HhaI GCGC 2 cut(s) 493, 869
Hin1II CATG 5 cut(s) 95, 409, 770, 1013, 1022
Hin6I GCGC 2 cut(s) 491, 867
HinP1I GCGC 2 cut(s) 491, 867
HphI GGTGA 5 cut(s) 257, 444, 488, 535, 928
Hpy166II GTNNAC 2 cut(s) 128, 644
Hpy188I TCNGA 6 cut(s) 28, 69, 294, 811, 1046, 1058
Hpy188III TCNNGA 6 cut(s) 14, 20, 110, 224, 598, 778
Hpy8I GTNNAC 2 cut(s) 128, 644
HpyAV CCTTC 5 cut(s) 362, 465, 698, 786, 917
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4IV ACGT 3 cut(s) 306, 987, 1041
HpyCH4V TGCA 3 cut(s) 263, 285, 327
HpyF10VI GCNNNNNNNGC 5 cut(s) 148, 205, 346, 614, 937
HpyF3I CTNAG 2 cut(s) 57, 686
HpySE526I ACGT 3 cut(s) 306, 987, 1041
Hsp92II CATG 5 cut(s) 95, 409, 770, 1013, 1022
HspAI GCGC 2 cut(s) 491, 867
Kpn2I TCCGGA 1 cut(s) 597
KroI GCCGGC 1 cut(s) 487
KroNI GCCGGC 1 cut(s) 489
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 7 cut(s) 16, 408, 421, 762, 780, 972, 1015
LmnI GCTCC 6 cut(s) 139, 217, 476, 671, 953, 1022
Lsp1109I GCAGC 2 cut(s) 856, 883
LweI GCATC 4 cut(s) 64, 229, 272, 1024
MaeI CTAG 4 cut(s) 224, 267, 426, 884
MaeII ACGT 3 cut(s) 306, 987, 1041
MaeIII GTNAC 4 cut(s) 450, 476, 523, 565
MalI GATC 7 cut(s) 18, 410, 423, 764, 782, 974, 1017
MboI GATC 7 cut(s) 16, 408, 421, 762, 780, 972, 1015
MboII GAAGA 3 cut(s) 262, 364, 367
MflI RGATCY 2 cut(s) 421, 780
MhlI GDGCHC 2 cut(s) 473, 942
MluCI AATT 4 cut(s) 280, 437, 630, 758
MlyI GAGTC 4 cut(s) 504, 783, 912, 1041
MmeI TCCRAC 1 cut(s) 814
MroI TCCGGA 1 cut(s) 597
MroNI GCCGGC 1 cut(s) 487
MseI TTAA 2 cut(s) 63, 213
MspA1I CMGCKG 1 cut(s) 208
MspR9I CCNGG 4 cut(s) 557, 852, 909, 980
MvaI CCWGG 2 cut(s) 852, 909
MvnI CGCG 1 cut(s) 493
MwoI GCNNNNNNNGC 5 cut(s) 148, 205, 346, 614, 937
NaeI GCCGGC 1 cut(s) 489
NciI CCSGG 2 cut(s) 557, 980
NdeII GATC 7 cut(s) 16, 408, 421, 762, 780, 972, 1015
NgoMIV GCCGGC 1 cut(s) 487
NlaIII CATG 5 cut(s) 95, 409, 770, 1013, 1022
NlaIV GGNNCC 1 cut(s) 561
NmeAIII GCCGAG 1 cut(s) 898
NmuCI GTSAC 3 cut(s) 450, 476, 523
NspI RCATGY 1 cut(s) 95
PasI CCCWGGG 2 cut(s) 851, 908
PciI ACATGT 1 cut(s) 91
PcsI WCGNNNNNNNCGW 2 cut(s) 726, 1038
PdiI GCCGGC 1 cut(s) 489
PfeI GAWTC 9 cut(s) 23, 106, 531, 552, 594, 601, 649, 690, 753
PflFI GACNNNGTC 1 cut(s) 773
PflMI CCANNNNNTGG 1 cut(s) 857
PfoI TCCNGGA 1 cut(s) 555
PinAI ACCGGT 1 cut(s) 562
PkrI GCNGC 2 cut(s) 871, 898
PleI GAGTC 4 cut(s) 504, 782, 911, 1041
PpsI GAGTC 4 cut(s) 504, 782, 911, 1041
PpuMI RGGWCCY 1 cut(s) 1003
PscI ACATGT 1 cut(s) 91
Psp5II RGGWCCY 1 cut(s) 1003
Psp6I CCWGG 2 cut(s) 850, 907
PspEI GGTNACC 3 cut(s) 476, 523, 565
PspFI CCCAGC 1 cut(s) 857
PspGI CCWGG 2 cut(s) 850, 907
PspN4I GGNNCC 1 cut(s) 561
PspPI GGNCC 1 cut(s) 1003
PspPPI RGGWCCY 1 cut(s) 1003
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 2 cut(s) 421, 780
PsyI GACNNNGTC 1 cut(s) 773
RsaI GTAC 2 cut(s) 257, 385
RsaNI GTAC 2 cut(s) 256, 384
SaqAI TTAA 2 cut(s) 63, 213
SatI GCNGC 2 cut(s) 870, 897
Sau3AI GATC 7 cut(s) 16, 408, 421, 762, 780, 972, 1015
Sau96I GGNCC 1 cut(s) 1003
SchI GAGTC 4 cut(s) 504, 783, 912, 1041
ScrFI CCNGG 4 cut(s) 557, 852, 909, 980
SduI GDGCHC 2 cut(s) 473, 942
SfaNI GCATC 4 cut(s) 64, 229, 272, 1024
SfcI CTRYAG 1 cut(s) 744
SinI GGWCC 1 cut(s) 1003
SmlI CTYRAG 2 cut(s) 157, 391
SmoI CTYRAG 2 cut(s) 157, 391
Sse9I AATT 4 cut(s) 280, 437, 630, 758
SsiI CCGC 6 cut(s) 208, 493, 696, 830, 963, 1013
SspMI CTAG 4 cut(s) 224, 267, 426, 884
StyD4I CCNGG 4 cut(s) 555, 850, 907, 978
TaaI ACNGT 1 cut(s) 523
TaiI ACGT 3 cut(s) 309, 990, 1044
TaqI TCGA 6 cut(s) 626, 720, 756, 777, 975, 1032
TaqII GACCGA 1 cut(s) 598
TasI AATT 4 cut(s) 280, 437, 630, 758
TfiI GAWTC 9 cut(s) 23, 106, 531, 552, 594, 601, 649, 690, 753
Tru1I TTAA 2 cut(s) 63, 213
Tru9I TTAA 2 cut(s) 63, 213
TscAI CASTG 4 cut(s) 329, 488, 651, 804
TseFI GTSAC 3 cut(s) 450, 476, 523
TseI GCWGC 2 cut(s) 869, 896
Tsp45I GTSAC 3 cut(s) 450, 476, 523
TspDTI ATGAA 1 cut(s) 102
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 4 cut(s) 329, 488, 651, 804
Tth111I GACNNNGTC 1 cut(s) 773
Van91I CCANNNNNTGG 1 cut(s) 857
VpaK11BI GGWCC 1 cut(s) 1003
XagI CCTNNNNNAGG 1 cut(s) 391
XapI RAATTY 2 cut(s) 437, 630
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 95
XcmI CCANNNNNNNNNTGG 1 cut(s) 1016
XspI CTAG 4 cut(s) 224, 267, 426, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.